DNA Topoisomerase I (eukaryota)
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT5G55300 AT5G55300 AT5G55300 |
| fragaria_vesca | FvH4_2g39170 |
| rosa_chinensis | RchiOBHm_Chr6g0301891 |
| rosa_laevigata | RLG00000011212 |
| rosa_multiflora | Rmu_sc0000407.1_g000003 |
| rosa_roxburghii | Rroxscaffold_7G00166300 |
| rosa_rugosa | Rorug06G0310500 |
| rosa_samantha | Rh6AG422700 Rh6BG429400 Rh6CG435900 Rh6DG422900 |
| rosa_wichuraiana | Rw6G036610 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 497 |
| Acc16I | TGCGCA | 1 cut(s) | 2361 |
| Acc36I | ACCTGC | 1 cut(s) | 418 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 1643 |
| AccII | CGCG | 3 cut(s) | 424, 2504, 2743 |
| AciI | CCGC | 6 cut(s) | 283, 424, 440, 1148, 1520, 2105 |
| AclWI | GGATC | 4 cut(s) | 1700, 1818, 2615, 2628 |
| AcsI | RAATTY | 4 cut(s) | 958, 1285, 1724, 1846 |
| AcuI | CTGAAG | 1 cut(s) | 732 |
| AdeI | CACNNNGTG | 1 cut(s) | 2357 |
| AfaI | GTAC | 7 cut(s) | 1181, 1787, 1969, 2209, 2338, 2661, 2732 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 423, 1091, 1559, 1643, 2462 |
| AflIII | ACRYGT | 2 cut(s) | 1324, 2502 |
| AjiI | CACGTC | 2 cut(s) | 1068, 2601 |
| AjnI | CCWGG | 5 cut(s) | 1522, 1685, 2100, 2182, 2616 |
| AleI | CACNNNNGTG | 1 cut(s) | 1131 |
| Alw21I | GWGCWC | 4 cut(s) | 1128, 1412, 1621, 2418 |
| Alw26I | GTCTC | 6 cut(s) | 42, 670, 1244, 2192, 2550, 2691 |
| Alw44I | GTGCAC | 1 cut(s) | 1124 |
| AlwI | GGATC | 4 cut(s) | 1700, 1818, 2615, 2628 |
| Ama87I | CYCGRG | 1 cut(s) | 2726 |
| AoxI | GGCC | 6 cut(s) | 410, 723, 1525, 1537, 1947, 2085 |
| ApaLI | GTGCAC | 1 cut(s) | 1124 |
| ApeKI | GCWGC | 2 cut(s) | 442, 974 |
| ApoI | RAATTY | 4 cut(s) | 958, 1285, 1724, 1846 |
| AspA2I | CCTAGG | 1 cut(s) | 1007 |
| AspLEI | GCGC | 3 cut(s) | 1915, 2362, 2745 |
| AspS9I | GGNCC | 2 cut(s) | 410, 1011 |
| AsuC2I | CCSGG | 1 cut(s) | 2435 |
| AsuII | TTCGAA | 2 cut(s) | 107, 209 |
| AsuNHI | GCTAGC | 1 cut(s) | 1690 |
| AvaI | CYCGRG | 1 cut(s) | 2726 |
| AvaII | GGWCC | 1 cut(s) | 1011 |
| AvrII | CCTAGG | 1 cut(s) | 1007 |
| BaeGI | GKGCMC | 3 cut(s) | 8, 1128, 2600 |
| BaeI | ACNNNNGTAYC | 4 cut(s) | 144, 177, 1322, 1355 |
| BamHI | GGATCC | 1 cut(s) | 2620 |
| BanII | GRGCYC | 1 cut(s) | 2418 |
| BbsI | GAAGAC | 2 cut(s) | 1062, 1312 |
| Bbv12I | GWGCWC | 4 cut(s) | 1128, 1412, 1621, 2418 |
| BbvI | GCAGC | 2 cut(s) | 454, 961 |
| BceAI | ACGGC | 2 cut(s) | 1524, 2730 |
| BciT130I | CCWGG | 5 cut(s) | 1524, 1687, 2102, 2184, 2618 |
| BciVI | GTATCC | 1 cut(s) | 1589 |
| BcnI | CCSGG | 1 cut(s) | 2435 |
| BcoDI | GTCTC | 6 cut(s) | 42, 670, 1244, 2192, 2550, 2691 |
| BfaI | CTAG | 5 cut(s) | 186, 1008, 1691, 1797, 2625 |
| BfmI | CTRYAG | 2 cut(s) | 222, 699 |
| BfoI | RGCGCY | 1 cut(s) | 1916 |
| BfuAI | ACCTGC | 1 cut(s) | 418 |
| BfuI | GTATCC | 1 cut(s) | 1589 |
| BisI | GCNGC | 3 cut(s) | 425, 443, 975 |
| BlnI | CCTAGG | 1 cut(s) | 1007 |
| BlpI | GCTNAGC | 1 cut(s) | 1769 |
| BlsI | GCNGC | 3 cut(s) | 426, 444, 976 |
| Bme1390I | CCNGG | 6 cut(s) | 1524, 1687, 2102, 2184, 2435, 2618 |
| Bme18I | GGWCC | 1 cut(s) | 1011 |
| BmeT110I | CYCGRG | 1 cut(s) | 2726 |
| BmgBI | CACGTC | 2 cut(s) | 1068, 2601 |
| BmgT120I | GGNCC | 2 cut(s) | 410, 1011 |
| BmiI | GGNNCC | 5 cut(s) | 199, 317, 631, 1518, 2622 |
| BmrFI | CCNGG | 6 cut(s) | 1524, 1687, 2102, 2184, 2435, 2618 |
| BmrI | ACTGGG | 1 cut(s) | 2603 |
| BmsI | GCATC | 1 cut(s) | 628 |
| BmtI | GCTAGC | 1 cut(s) | 1694 |
| BmuI | ACTGGG | 1 cut(s) | 2603 |
| BpiI | GAAGAC | 2 cut(s) | 1062, 1312 |
| BpmI | CTGGAG | 3 cut(s) | 66, 704, 1113 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1916 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 1769 |
| Bpu14I | TTCGAA | 2 cut(s) | 107, 209 |
| BpuEI | CTTGAG | 1 cut(s) | 2381 |
| BpuMI | CCSGG | 1 cut(s) | 2435 |
| BsaBI | GATNNNNATC | 1 cut(s) | 901 |
| BsaI | GGTCTC | 2 cut(s) | 670, 2192 |
| BsaJI | CCNNGG | 4 cut(s) | 156, 1007, 1540, 1857 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 566, 596 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 423, 1091, 1559, 1643, 2462 |
| Bse1I | ACTGG | 4 cut(s) | 319, 1304, 2044, 2598 |
| Bse3DI | GCAATG | 3 cut(s) | 278, 1026, 2718 |
| Bse8I | GATNNNNATC | 1 cut(s) | 901 |
| BseBI | CCWGG | 5 cut(s) | 1524, 1687, 2102, 2184, 2618 |
| BseDI | CCNNGG | 4 cut(s) | 156, 1007, 1540, 1857 |
| BseGI | GGATG | 9 cut(s) | 376, 567, 1348, 1549, 1582, 1867, 1942, 2239, 2722 |
| BseJI | GATNNNNATC | 1 cut(s) | 901 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 423, 1091, 1559, 1643, 2462 |
| BseMI | GCAATG | 3 cut(s) | 278, 1026, 2718 |
| BseMII | CTCAG | 3 cut(s) | 357, 1760, 2234 |
| BseNI | ACTGG | 4 cut(s) | 319, 1304, 2044, 2598 |
| BseRI | GAGGAG | 3 cut(s) | 1430, 1451, 1469 |
| BseSI | GKGCMC | 3 cut(s) | 8, 1128, 2600 |
| BseXI | GCAGC | 2 cut(s) | 454, 961 |
| Bsh1236I | CGCG | 3 cut(s) | 424, 2504, 2743 |
| BshFI | GGCC | 6 cut(s) | 412, 725, 1527, 1539, 1949, 2087 |
| BsiHKAI | GWGCWC | 4 cut(s) | 1128, 1412, 1621, 2418 |
| BsiHKCI | CYCGRG | 1 cut(s) | 2726 |
| BsiSI | CCGG | 1 cut(s) | 2434 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 423, 1091, 1559, 1643, 2462 |
| BsmAI | GTCTC | 6 cut(s) | 42, 670, 1244, 2192, 2550, 2691 |
| BsmI | GAATGC | 1 cut(s) | 2164 |
| BsnI | GGCC | 6 cut(s) | 412, 725, 1527, 1539, 1949, 2087 |
| Bso31I | GGTCTC | 2 cut(s) | 670, 2192 |
| BsoBI | CYCGRG | 1 cut(s) | 2726 |
| Bsp119I | TTCGAA | 2 cut(s) | 107, 209 |
| Bsp1286I | GDGCHC | 6 cut(s) | 8, 1128, 1412, 1621, 2418, 2600 |
| Bsp1407I | TGTACA | 1 cut(s) | 1179 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 1769 |
| BspACI | CCGC | 6 cut(s) | 283, 424, 440, 1148, 1520, 2105 |
| BspANI | GGCC | 6 cut(s) | 412, 725, 1527, 1539, 1949, 2087 |
| BspCNI | CTCAG | 3 cut(s) | 358, 1761, 2235 |
| BspFNI | CGCG | 3 cut(s) | 424, 2504, 2743 |
| BspHI | TCATGA | 1 cut(s) | 2653 |
| BspLI | GGNNCC | 5 cut(s) | 199, 317, 631, 1518, 2622 |
| BspMAI | CTGCAG | 1 cut(s) | 703 |
| BspMI | ACCTGC | 1 cut(s) | 418 |
| BspOI | GCTAGC | 1 cut(s) | 1694 |
| BspPI | GGATC | 4 cut(s) | 1700, 1818, 2615, 2628 |
| BspT104I | TTCGAA | 2 cut(s) | 107, 209 |
| BspTNI | GGTCTC | 2 cut(s) | 670, 2192 |
| BsrDI | GCAATG | 3 cut(s) | 278, 1026, 2718 |
| BsrGI | TGTACA | 1 cut(s) | 1179 |
| BsrI | ACTGG | 4 cut(s) | 319, 1304, 2044, 2598 |
| BssECI | CCNNGG | 4 cut(s) | 156, 1007, 1540, 1857 |
| BssT1I | CCWWGG | 3 cut(s) | 156, 1007, 1857 |
| Bst2UI | CCWGG | 5 cut(s) | 1524, 1687, 2102, 2184, 2618 |
| Bst6I | CTCTTC | 3 cut(s) | 424, 1212, 1377 |
| BstAUI | TGTACA | 1 cut(s) | 1179 |
| BstBI | TTCGAA | 2 cut(s) | 107, 209 |
| BstC8I | GCNNGC | 3 cut(s) | 487, 1692, 1750 |
| BstDEI | CTNAG | 9 cut(s) | 255, 366, 1035, 1769, 1916, 2243, 2491, 2535, 2604 |
| BstDSI | CCRYGG | 1 cut(s) | 1540 |
| BstF5I | GGATG | 9 cut(s) | 376, 567, 1348, 1549, 1582, 1867, 1942, 2239, 2722 |
| BstFNI | CGCG | 3 cut(s) | 424, 2504, 2743 |
| BstH2I | RGCGCY | 1 cut(s) | 1916 |
| BstHHI | GCGC | 3 cut(s) | 1915, 2362, 2745 |
| BstMAI | GTCTC | 6 cut(s) | 42, 670, 1244, 2192, 2550, 2691 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 266, 1156, 1309, 1700, 1919 |
| BstNI | CCWGG | 5 cut(s) | 1524, 1687, 2102, 2184, 2618 |
| BstNSI | RCATGY | 2 cut(s) | 1328, 2563 |
| BstSCI | CCNGG | 6 cut(s) | 1522, 1685, 2100, 2182, 2433, 2616 |
| BstSFI | CTRYAG | 2 cut(s) | 222, 699 |
| BstSLI | GKGCMC | 3 cut(s) | 8, 1128, 2600 |
| BstUI | CGCG | 3 cut(s) | 424, 2504, 2743 |
| BstV1I | GCAGC | 2 cut(s) | 454, 961 |
| BstV2I | GAAGAC | 2 cut(s) | 1062, 1312 |
| BstX2I | RGATCY | 1 cut(s) | 2620 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 2230, 2505 |
| BstYI | RGATCY | 1 cut(s) | 2620 |
| BsuI | GTATCC | 1 cut(s) | 1589 |
| BsuRI | GGCC | 6 cut(s) | 412, 725, 1527, 1539, 1949, 2087 |
| BtgI | CCRYGG | 1 cut(s) | 1540 |
| BtgZI | GCGATG | 1 cut(s) | 2280 |
| BtrI | CACGTC | 2 cut(s) | 1068, 2601 |
| BtsCI | GGATG | 9 cut(s) | 376, 567, 1348, 1549, 1582, 1867, 1942, 2239, 2722 |
| BtsI | GCAGTG | 1 cut(s) | 1245 |
| BtsIMutI | CAGTG | 8 cut(s) | 6, 58, 99, 756, 1245, 1953, 2591, 2592 |
| BveI | ACCTGC | 1 cut(s) | 418 |
| Cac8I | GCNNGC | 3 cut(s) | 487, 1692, 1750 |
| CciI | TCATGA | 1 cut(s) | 2653 |
| CfoI | GCGC | 3 cut(s) | 1915, 2362, 2745 |
| Cfr13I | GGNCC | 2 cut(s) | 410, 1011 |
| Csp6I | GTAC | 7 cut(s) | 1180, 1786, 1968, 2208, 2337, 2660, 2731 |
| CviAII | CATG | 9 cut(s) | 356, 1163, 1325, 1587, 1670, 2354, 2560, 2640, 2654 |
| CviQI | GTAC | 7 cut(s) | 1180, 1786, 1968, 2208, 2337, 2660, 2731 |
| DdeI | CTNAG | 9 cut(s) | 255, 366, 1035, 1769, 1916, 2243, 2491, 2535, 2604 |
| DraI | TTTAAA | 1 cut(s) | 1974 |
| DraIII | CACNNNGTG | 1 cut(s) | 2357 |
| Eam1104I | CTCTTC | 3 cut(s) | 424, 1212, 1377 |
| EarI | CTCTTC | 3 cut(s) | 424, 1212, 1377 |
| Ecl136II | GAGCTC | 1 cut(s) | 2416 |
| Eco130I | CCWWGG | 3 cut(s) | 156, 1007, 1857 |
| Eco24I | GRGCYC | 1 cut(s) | 2418 |
| Eco31I | GGTCTC | 2 cut(s) | 670, 2192 |
| Eco32I | GATATC | 1 cut(s) | 888 |
| Eco47I | GGWCC | 1 cut(s) | 1011 |
| Eco53kI | GAGCTC | 1 cut(s) | 2416 |
| Eco57I | CTGAAG | 1 cut(s) | 732 |
| Eco88I | CYCGRG | 1 cut(s) | 2726 |
| EcoICRI | GAGCTC | 1 cut(s) | 2416 |
| EcoRI | GAATTC | 1 cut(s) | 1724 |
| EcoRII | CCWGG | 5 cut(s) | 1522, 1685, 2100, 2182, 2616 |
| EcoRV | GATATC | 1 cut(s) | 888 |
| EcoT14I | CCWWGG | 3 cut(s) | 156, 1007, 1857 |
| EcoT22I | ATGCAT | 1 cut(s) | 2565 |
| EcoT38I | GRGCYC | 1 cut(s) | 2418 |
| ErhI | CCWWGG | 3 cut(s) | 156, 1007, 1857 |
| FaeI | CATG | 9 cut(s) | 359, 1166, 1328, 1590, 1673, 2357, 2563, 2643, 2657 |
| FatI | CATG | 9 cut(s) | 355, 1162, 1324, 1586, 1669, 2353, 2559, 2639, 2653 |
| FauI | CCCGC | 1 cut(s) | 2098 |
| Fnu4HI | GCNGC | 3 cut(s) | 425, 443, 975 |
| FokI | GGATG | 9 cut(s) | 383, 574, 1355, 1536, 1569, 1874, 1949, 2246, 2729 |
| FriOI | GRGCYC | 1 cut(s) | 2418 |
| Fsp4HI | GCNGC | 3 cut(s) | 425, 443, 975 |
| FspBI | CTAG | 5 cut(s) | 186, 1008, 1691, 1797, 2625 |
| FspI | TGCGCA | 1 cut(s) | 2361 |
| GlaI | GCGC | 3 cut(s) | 1914, 2361, 2744 |
| GluI | GCNGC | 3 cut(s) | 425, 443, 975 |
| GsuI | CTGGAG | 3 cut(s) | 66, 704, 1113 |
| HaeII | RGCGCY | 1 cut(s) | 1916 |
| HaeIII | GGCC | 6 cut(s) | 412, 725, 1527, 1539, 1949, 2087 |
| HapII | CCGG | 1 cut(s) | 2434 |
| HhaI | GCGC | 3 cut(s) | 1915, 2362, 2745 |
| Hin1II | CATG | 9 cut(s) | 359, 1166, 1328, 1590, 1673, 2357, 2563, 2643, 2657 |
| Hin6I | GCGC | 3 cut(s) | 1913, 2360, 2743 |
| HinP1I | GCGC | 3 cut(s) | 1913, 2360, 2743 |
| HincII | GTYRAC | 1 cut(s) | 1198 |
| HindII | GTYRAC | 1 cut(s) | 1198 |
| HindIII | AAGCTT | 2 cut(s) | 1425, 2488 |
| HpaII | CCGG | 1 cut(s) | 2434 |
| Hpy166II | GTNNAC | 5 cut(s) | 1126, 1198, 2053, 2080, 2153 |
| Hpy8I | GTNNAC | 5 cut(s) | 1126, 1198, 2053, 2080, 2153 |
| HpyAV | CCTTC | 8 cut(s) | 216, 715, 792, 1042, 1095, 1927, 2164, 2456 |
| HpyCH4IV | ACGT | 3 cut(s) | 1067, 2600, 2650 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 266, 1156, 1309, 1700, 1919 |
| HpyF3I | CTNAG | 9 cut(s) | 255, 366, 1035, 1769, 1916, 2243, 2491, 2535, 2604 |
| HpySE526I | ACGT | 3 cut(s) | 1067, 2600, 2650 |
| Hsp92II | CATG | 9 cut(s) | 359, 1166, 1328, 1590, 1673, 2357, 2563, 2643, 2657 |
| HspAI | GCGC | 3 cut(s) | 1913, 2360, 2743 |
| LmnI | GCTCC | 3 cut(s) | 315, 1624, 1745 |
| Lsp1109I | GCAGC | 2 cut(s) | 454, 961 |
| LweI | GCATC | 1 cut(s) | 628 |
| MaeI | CTAG | 5 cut(s) | 186, 1008, 1691, 1797, 2625 |
| MaeII | ACGT | 3 cut(s) | 1067, 2600, 2650 |
| MaeIII | GTNAC | 8 cut(s) | 181, 273, 321, 920, 1042, 1349, 1681, 2269 |
| MfeI | CAATTG | 1 cut(s) | 1638 |
| MflI | RGATCY | 1 cut(s) | 2620 |
| MhlI | GDGCHC | 6 cut(s) | 8, 1128, 1412, 1621, 2418, 2600 |
| MluI | ACGCGT | 1 cut(s) | 2502 |
| MlyI | GAGTC | 3 cut(s) | 515, 1046, 2254 |
| MmeI | TCCRAC | 3 cut(s) | 939, 1320, 1960 |
| Mph1103I | ATGCAT | 1 cut(s) | 2565 |
| MseI | TTAA | 9 cut(s) | 306, 383, 639, 1170, 1601, 1713, 1866, 1973, 2409 |
| MslI | CAYNNNNRTG | 2 cut(s) | 624, 1131 |
| MspA1I | CMGCKG | 1 cut(s) | 974 |
| MspI | CCGG | 1 cut(s) | 2434 |
| MspR9I | CCNGG | 6 cut(s) | 1524, 1687, 2102, 2184, 2435, 2618 |
| MunI | CAATTG | 1 cut(s) | 1638 |
| Mva1269I | GAATGC | 1 cut(s) | 2164 |
| MvaI | CCWGG | 5 cut(s) | 1524, 1687, 2102, 2184, 2618 |
| MvnI | CGCG | 3 cut(s) | 424, 2504, 2743 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 266, 1156, 1309, 1700, 1919 |
| NciI | CCSGG | 1 cut(s) | 2435 |
| NheI | GCTAGC | 1 cut(s) | 1690 |
| NlaIII | CATG | 9 cut(s) | 359, 1166, 1328, 1590, 1673, 2357, 2563, 2643, 2657 |
| NlaIV | GGNNCC | 5 cut(s) | 199, 317, 631, 1518, 2622 |
| NmuCI | GTSAC | 4 cut(s) | 181, 273, 1349, 1681 |
| NsbI | TGCGCA | 1 cut(s) | 2361 |
| NsiI | ATGCAT | 1 cut(s) | 2565 |
| NspI | RCATGY | 2 cut(s) | 1328, 2563 |
| NspV | TTCGAA | 2 cut(s) | 107, 209 |
| OliI | CACNNNNGTG | 1 cut(s) | 1131 |
| PagI | TCATGA | 1 cut(s) | 2653 |
| PciI | ACATGT | 1 cut(s) | 1324 |
| PctI | GAATGC | 1 cut(s) | 2164 |
| PfeI | GAWTC | 9 cut(s) | 109, 362, 767, 897, 908, 1801, 2036, 2628, 2736 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 1643 |
| PkrI | GCNGC | 3 cut(s) | 426, 444, 976 |
| PleI | GAGTC | 3 cut(s) | 514, 1046, 2253 |
| PpsI | GAGTC | 3 cut(s) | 514, 1046, 2253 |
| PscI | ACATGT | 1 cut(s) | 1324 |
| PsiI | TTATAA | 1 cut(s) | 497 |
| Psp124BI | GAGCTC | 1 cut(s) | 2418 |
| Psp6I | CCWGG | 5 cut(s) | 1522, 1685, 2100, 2182, 2616 |
| PspGI | CCWGG | 5 cut(s) | 1522, 1685, 2100, 2182, 2616 |
| PspN4I | GGNNCC | 5 cut(s) | 199, 317, 631, 1518, 2622 |
| PspPI | GGNCC | 2 cut(s) | 410, 1011 |
| PstI | CTGCAG | 1 cut(s) | 703 |
| PsuI | RGATCY | 1 cut(s) | 2620 |
| PvuII | CAGCTG | 1 cut(s) | 974 |
| RsaI | GTAC | 7 cut(s) | 1181, 1787, 1969, 2209, 2338, 2661, 2732 |
| RsaNI | GTAC | 7 cut(s) | 1180, 1786, 1968, 2208, 2337, 2660, 2731 |
| RseI | CAYNNNNRTG | 2 cut(s) | 624, 1131 |
| SacI | GAGCTC | 1 cut(s) | 2418 |
| SaqAI | TTAA | 9 cut(s) | 306, 383, 639, 1170, 1601, 1713, 1866, 1973, 2409 |
| SatI | GCNGC | 3 cut(s) | 425, 443, 975 |
| Sau96I | GGNCC | 2 cut(s) | 410, 1011 |
| SchI | GAGTC | 3 cut(s) | 515, 1046, 2254 |
| ScrFI | CCNGG | 6 cut(s) | 1524, 1687, 2102, 2184, 2435, 2618 |
| SduI | GDGCHC | 6 cut(s) | 8, 1128, 1412, 1621, 2418, 2600 |
| SfaNI | GCATC | 1 cut(s) | 628 |
| SfcI | CTRYAG | 2 cut(s) | 222, 699 |
| SfuI | TTCGAA | 2 cut(s) | 107, 209 |
| SinI | GGWCC | 1 cut(s) | 1011 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 624, 1131 |
| SmlI | CTYRAG | 1 cut(s) | 2396 |
| SmoI | CTYRAG | 1 cut(s) | 2396 |
| SsiI | CCGC | 6 cut(s) | 283, 424, 440, 1148, 1520, 2105 |
| SspI | AATATT | 2 cut(s) | 1061, 1140 |
| SspMI | CTAG | 5 cut(s) | 186, 1008, 1691, 1797, 2625 |
| SstI | GAGCTC | 1 cut(s) | 2418 |
| StyD4I | CCNGG | 6 cut(s) | 1522, 1685, 2100, 2182, 2433, 2616 |
| StyI | CCWWGG | 3 cut(s) | 156, 1007, 1857 |
| TaiI | ACGT | 3 cut(s) | 1070, 2603, 2653 |
| TaqI | TCGA | 6 cut(s) | 107, 209, 469, 2061, 2541, 2667 |
| TatI | WGTACW | 3 cut(s) | 1179, 1967, 2730 |
| TauI | GCSGC | 1 cut(s) | 427 |
| TfiI | GAWTC | 9 cut(s) | 109, 362, 767, 897, 908, 1801, 2036, 2628, 2736 |
| Tru1I | TTAA | 9 cut(s) | 306, 383, 639, 1170, 1601, 1713, 1866, 1973, 2409 |
| Tru9I | TTAA | 9 cut(s) | 306, 383, 639, 1170, 1601, 1713, 1866, 1973, 2409 |
| TscAI | CASTG | 8 cut(s) | 13, 58, 99, 756, 1245, 1960, 2592, 2598 |
| TseFI | GTSAC | 4 cut(s) | 181, 273, 1349, 1681 |
| TseI | GCWGC | 2 cut(s) | 442, 974 |
| Tsp45I | GTSAC | 4 cut(s) | 181, 273, 1349, 1681 |
| TspDTI | ATGAA | 9 cut(s) | 531, 606, 660, 773, 836, 957, 1460, 2250, 2670 |
| TspGWI | ACGGA | 2 cut(s) | 1823, 2195 |
| TspRI | CASTG | 8 cut(s) | 13, 58, 99, 756, 1245, 1960, 2592, 2598 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 1643 |
| VneI | GTGCAC | 1 cut(s) | 1124 |
| VpaK11BI | GGWCC | 1 cut(s) | 1011 |
| XapI | RAATTY | 4 cut(s) | 958, 1285, 1724, 1846 |
| XceI | RCATGY | 2 cut(s) | 1328, 2563 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 2689 |
| XmaJI | CCTAGG | 1 cut(s) | 1007 |
| XspI | CTAG | 5 cut(s) | 186, 1008, 1691, 1797, 2625 |
| Zsp2I | ATGCAT | 1 cut(s) | 2565 |