Rorug06G0310500

LON peptidase N-terminal domain and RING finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0004341
Physical Location & Seq
Reverse (-)
46173275 .. 46180993
7719 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0310500.1

Sequence Viewer

Length: 2937 bp
ATGGCGGACCCTCAGAACCAGAAGCCATGGAAGGTCGAGTACGCCAAGTCTTCACGATCGTCATGCAAAACCTGCAGAAACCCCATTGAAAAAGAGAACCTCCGCTTCGGCAAAATGGTCCAGGCCAAACAATTCGACGGCTTCATGCCCATGTGGAACCATGCTAGTTGCATAATGAAGAAGGCGAAGCAGATAAAATCAACTGATGATGTTGAAGGCCTCGAGCTCCTCCGGTGGGAAGATCAGAAGAAAATTAGAGATTATGTACAAAGTGGTGGATCTGGTGGACCCTCGGATACAACAACTACAAGCAAGGCCAGTGCTACAGAAAGTGGTATTGAAGTTTCGCAAACTTCTCGGGCTACTTGCAGGCTCTGCAGCCAAAAGATTTTAAAAGGGGAGGTCCGTATATCAACCAAGCCTGAAGGTCAAGGAGCTAGGGGCTTGGCATGGCACCATGCCAAGTGTTTCATGGAATCATCACCATCTACCCAAGTGGAGAAGCTGTCTGGATGGGAGACCATCCCAGTTTCTGATCAGGCAGCTGTGTCTGCCTTAGTTAAGGATGGCAAAGAAGTTAAAGAGATCCTGCCACAGTCAACTTCTAAAGCTGGCACTAAACGAAGAAAAGATGGGGATGGTGATCAGAAGTCAAAAGTTTCTAAGTCGGAAGGTGACGTGTCAACAAGCAGGGATGTGTCTGTGAGTAATGCTACTGATATGGAGAGCAAAATGGAGGCACAAACTAAAGAGCTGTGGGCTCTGAAAGATGATCTCAAAAAGCATGTGACAACTGTAGAGCTGCGTAAAATGCTTGAAGCCAATGCTCAAGATTCAACAGGATCAGAGCTTGATTTGCGTGAGCGCTGCGCTGATGGAATGATGTTTGGAGCACTTAGTACCTGCCCACTCTGTTCCGATCATCTTCACTATTCTGGAGCCATGTACAGGTGCCATGGGTTCCTGACTGCATGGAGCAAGTGTTCTTACTCTACTCAAGAAACTGAACGTCGTAAAGGGAAGTGGAAAATCCCAGAAGATACAGATAATCAATTTCTGCAGAAGTGGTTTAAATCACAGAAGGTTAGCAAACCTACACGAATACTGCCACCGCCATCATCTAACAATCCTGGAGGTCAAGCTTTCAATGGCCAATCTCAGTCTGGTGCAAGCTTGGGGGATTTGAAAGTTTCTTTTCGTGGATTACCAAAAGAATCCATGGAAAAGTGGAATAAAGATATAGAGGGTGTAGCTGGGTCCGTTCATGCTAAGATAAAAAAAGATACTAATTGCTTGGTTGTGGGTGGAGGACTGGATGATAAAGATGCTGAGATGAAAAAAGCAAGGAGGATGAAAATACCAATTGTTAGGGAGGATTATCTGGTTGATTGCTTTAAAAGAAAGAAGAAGCTTCCATTTGATCTTTACAAAGTTGAAGCTGTTGGTGAAGCCTCTAGCATGGTCACTGTCAAAGTGAAAGGGCGAAGTGCCGTTCATGAATCTTCTGGATTGCAAGATACAGGTCACATACTTGAGGATGGAAAAAGCATATATAATACAACTTTAAGCATGTCTGATTTGTCAACCGGTGTTAATAGTTATTACATCCTCCAAATTATACAAGATGATAAAAGTTCAGACTGCCATGTGTTCCGTAAATGGGGACGAGTAGGCAATGACAAAATTGGAGGAACCAAACTAGAAGAGATGTCAAAATATGATGCCATATCTGATTTTAAACGTTTGTTTCTTGAGAAGACTGGCAATTCATGGGAAGCATGGGAGAAAAAGCAAGATTTTCGGAAGCAACCTGGAAAATTCTTTCCATTGGATATTGATTATGGTGTTAACAAAGAGGTTTCAAAGAAAAATCAGAACAATGAACCCAGCAAATTGCCTCCTCAGTTAGCAGAGTTGATGAAGATGCTCTTCAACGTTGAAACATACAGAGCTGCCATGATGGAATTTGAGATAAATATGTCAGAGATGCCACTTGGAAAACTGAGTAAAAGCAATATCCAAAAGGGGTTTGAGGCATTAACGGAAATACAGAATCTATTAAAGAGTGATGGTGCCTCTTCCATAAAAGATAGCTTGATTGTAGATGCAAGCAATCGATTTTTCACAGTGATACCTTCCATACATCCGCATATTATTAGGGATGAGGATGACTTTAAGTCAAAGTTGAAAATGCTAGAAGCTCTCCAGGACATTGAAATTGCTTCCAGATTAGTCGGTTTTGATGCTGATAGTGATGACTCTTTGGATGAGAAGTATAAGAAGCTCCAGTGTTGTATGAATCCACTTCCTCACGATAGTGAAGATTATCGGTTGATTGAAAAGTATCTTCTTACTACTCATGCCCCTACACATACGGATTGGAGTCTTGAACTTGAAGAAGTTTTTTCACTTGAACGGGAAGGGGAATTCGATAAATACGCTCCTTACCGGAAAAATCTTAAAAACAGAATGCTGCTTTGGCATGGTTCTCGGTTTACAAACTTTGTGGGAATACTTAGCCAAGGACTAAGAATAGCTCCTCCTGAAGCTCCAGCTACTGGTTATATGTTTGGCAAGGGGATTTACTTTGCTGACCTTGTCAGTAAGAGTGCTCAGTATTGCTATACAGATAAGAAAAATCCTGTGGGCCTCATGCTTTTAAGTGAAGTTGCCCTGGGAGAGATCCATGAGCTCAAGAAGGCCACATATATGGATAAACCTCCTAAGGGAAAGCACTCTACGAAGGGACTTGGCAAGAAAAAACCTGAGGAGTCGGACTATGTTAAATGGAGAGATGATGTCACTGTGCCCTGTGGCAAACCAGTGGATTCACATGTCAGGGCCTCTGAGCTGATGTATAATGAGTATATTGTTTATGATACAGCTCAAGTTAAGATGCAATTTCTATTGAAGGTGAAGTTTCATCACAAGAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LON_substr_bdg

Protein Analysis

978

Amino Acids

110.23

Weight (kDa)

8.8

Isoelectric Point (pI)

42.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-PARP PF00645 13 - 88 6.7e-22 Poly(ADP-ribose) polymerase and DNA-Ligase Zn-finger region
zf-PARP PF00645 114 - 185 4.8e-12 Poly(ADP-ribose) polymerase and DNA-Ligase Zn-finger region
PADR1_N PF21728 239 - 297 1e-21 PADR1, N-terminal helical domain
Zn_ribbon_PADR1 PF08063 298 - 337 2.7e-12 PADR1 domain, zinc ribbon fold
BRCT PF00533 394 - 465 2.3e-08 BRCA1 C Terminus (BRCT) domain
WGR PF05406 518 - 597 8e-17 WGR domain
PARP_reg PF02877 630 - 759 4.8e-37 Poly(ADP-ribose) polymerase, regulatory domain
PARP PF00644 773 - 974 8.8e-82 Poly(ADP-ribose) polymerase catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 80, 911
AccB1I GGYRCC 3 cut(s) 453, 951, 2072
AccB7I CCANNNNNTGG 1 cut(s) 2558
AciI CCGC 4 cut(s) 5, 103, 1112, 2147
AclI AACGTT 2 cut(s) 1741, 1935
AclWI GGATC 4 cut(s) 286, 580, 850, 2677
AcoI YGGCCR 1 cut(s) 1150
AcsI RAATTY 3 cut(s) 1817, 1964, 2426
AcuI CTGAAG 2 cut(s) 444, 2565
AfaI GTAC 4 cut(s) 41, 267, 901, 947
AfeI AGCGCT 1 cut(s) 866
AfiI CCNNNNNNNGG 5 cut(s) 235, 948, 1660, 2558, 2726
AflIII ACRYGT 2 cut(s) 678, 2833
AgeI ACCGGT 1 cut(s) 1586
AhdI GACNNNNNGTC 1 cut(s) 2176
AjiI CACGTC 1 cut(s) 679
AjnI CCWGG 5 cut(s) 120, 1129, 1810, 2205, 2673
AjuI GAANNNNNNNTTGG 2 cut(s) 2461, 2493
AleI CACNNNNGTG 1 cut(s) 2316
AloI GAACNNNNNNTCC 2 cut(s) 967, 999
Alw21I GWGCWC 4 cut(s) 228, 895, 2614, 2694
Alw26I GTCTC 1 cut(s) 512
AlwI GGATC 4 cut(s) 286, 580, 850, 2677
AlwNI CAGNNNCTG 3 cut(s) 375, 533, 2558
Ama87I CYCGRG 2 cut(s) 221, 357
Aor51HI AGCGCT 1 cut(s) 866
AoxI GGCC 7 cut(s) 123, 217, 315, 1150, 2647, 2700, 2841
ApeKI GCWGC 6 cut(s) 378, 542, 802, 867, 1952, 2473
ApoI RAATTY 3 cut(s) 1817, 1964, 2426
AsiGI ACCGGT 1 cut(s) 1586
AspLEI GCGC 2 cut(s) 867, 872
AspS9I GGNCC 7 cut(s) 7, 118, 287, 403, 1257, 2647, 2841
AsuHPI GGTGA 5 cut(s) 474, 653, 686, 1457, 2926
AvaI CYCGRG 2 cut(s) 221, 357
AvaII GGWCC 5 cut(s) 7, 118, 287, 403, 1257
AxyI CCTNAGG 2 cut(s) 2724, 2766
BaeGI GKGCMC 1 cut(s) 2811
BalI TGGCCA 1 cut(s) 1152
BanI GGYRCC 3 cut(s) 453, 951, 2072
BanII GRGCYC 3 cut(s) 228, 763, 2694
BarI GAAGNNNNNNTAC 2 cut(s) 23, 55
BbsI GAAGAC 2 cut(s) 42, 1763
Bbv12I GWGCWC 4 cut(s) 228, 895, 2614, 2694
BbvI GCAGC 6 cut(s) 390, 554, 789, 854, 1939, 2460
BceAI ACGGC 2 cut(s) 154, 1475
BcgI CGANNNNNNTGC 8 cut(s) 45, 79, 338, 372, 1781, 1815, 2471, 2505
BciT130I CCWGG 5 cut(s) 122, 1131, 1812, 2207, 2675
BciVI GTATCC 1 cut(s) 289
BclI TGATCA 2 cut(s) 535, 643
BcoDI GTCTC 1 cut(s) 512
BfaI CTAG 5 cut(s) 165, 438, 1455, 1700, 2195
BfmI CTRYAG 5 cut(s) 73, 324, 376, 795, 1058
BfoI RGCGCY 1 cut(s) 868
BfuAI ACCTGC 2 cut(s) 80, 911
BfuI GTATCC 1 cut(s) 289
BisI GCNGC 6 cut(s) 379, 543, 803, 868, 1953, 2474
BlsI GCNGC 6 cut(s) 380, 544, 804, 869, 1954, 2475
Bme1390I CCNGG 5 cut(s) 122, 1131, 1812, 2207, 2675
Bme18I GGWCC 5 cut(s) 7, 118, 287, 403, 1257
BmeRI GACNNNNNGTC 1 cut(s) 2176
BmeT110I CYCGRG 2 cut(s) 221, 357
BmgBI CACGTC 1 cut(s) 679
BmgT120I GGNCC 7 cut(s) 7, 118, 287, 403, 1257, 2647, 2841
BmrFI CCNGG 5 cut(s) 122, 1131, 1812, 2207, 2675
BmrI ACTGGG 1 cut(s) 521
BmsI GCATC 7 cut(s) 1315, 1711, 1914, 1977, 2095, 2233, 2886
BmuI ACTGGG 1 cut(s) 521
BpiI GAAGAC 2 cut(s) 42, 1763
BpmI CTGGAG 5 cut(s) 957, 1152, 2189, 2270, 2535
BpuEI CTTGAG 6 cut(s) 813, 981, 1553, 1772, 2678, 2871
Bsa29I ATCGAT 1 cut(s) 2116
BsaBI GATNNNNATC 1 cut(s) 642
BsaI GGTCTC 1 cut(s) 512
BsaJI CCNNGG 7 cut(s) 26, 291, 955, 1218, 2521, 2673, 2674
BsaWI WCCGGW 3 cut(s) 231, 1586, 2448
BsaXI ACNNNNNCTCC 4 cut(s) 491, 521, 967, 997
Bsc4I CCNNNNNNNGG 5 cut(s) 235, 948, 1660, 2558, 2726
Bse118I RCCGGY 1 cut(s) 1586
Bse1I ACTGG 7 cut(s) 318, 527, 1317, 1765, 2287, 2563, 2822
Bse21I CCTNAGG 2 cut(s) 2724, 2766
Bse3DI GCAATG 1 cut(s) 1681
Bse8I GATNNNNATC 1 cut(s) 642
BseBI CCWGG 5 cut(s) 122, 1131, 1812, 2207, 2675
BseCI ATCGAT 1 cut(s) 2116
BseDI CCNNGG 7 cut(s) 26, 291, 955, 1218, 2521, 2673, 2674
BseJI GATNNNNATC 1 cut(s) 642
BseLI CCNNNNNNNGG 5 cut(s) 235, 948, 1660, 2558, 2726
BseMI GCAATG 1 cut(s) 1681
BseMII CTCAG 8 cut(s) 26, 1172, 1320, 1916, 1994, 2627, 2757, 2838
BseNI ACTGG 7 cut(s) 318, 527, 1317, 1765, 2287, 2563, 2822
BseRI GAGGAG 4 cut(s) 218, 1890, 2529, 2783
BseSI GKGCMC 1 cut(s) 2811
BseXI GCAGC 6 cut(s) 390, 554, 789, 854, 1939, 2460
BseYI CCCAGC 2 cut(s) 1253, 1886
Bsh1285I CGRYCG 1 cut(s) 59
BshFI GGCC 7 cut(s) 125, 219, 317, 1152, 2649, 2702, 2843
BshNI GGYRCC 3 cut(s) 453, 951, 2072
BshTI ACCGGT 1 cut(s) 1586
BshVI ATCGAT 1 cut(s) 2116
BsiEI CGRYCG 1 cut(s) 59
BsiHKAI GWGCWC 4 cut(s) 228, 895, 2614, 2694
BsiHKCI CYCGRG 2 cut(s) 221, 357
BsiSI CCGG 3 cut(s) 232, 1587, 2449
BslFI GGGAC 2 cut(s) 1677, 2760
BslI CCNNNNNNNGG 5 cut(s) 235, 948, 1660, 2558, 2726
BsmAI GTCTC 1 cut(s) 512
BsmFI GGGAC 2 cut(s) 1677, 2760
BsmI GAATGC 1 cut(s) 2475
BsnI GGCC 7 cut(s) 125, 219, 317, 1152, 2649, 2702, 2843
Bso31I GGTCTC 1 cut(s) 512
BsoBI CYCGRG 2 cut(s) 221, 357
Bsp1286I GDGCHC 6 cut(s) 228, 763, 895, 2614, 2694, 2811
Bsp1407I TGTACA 2 cut(s) 265, 945
Bsp19I CCATGG 3 cut(s) 26, 955, 1218
BspACI CCGC 4 cut(s) 5, 103, 1112, 2147
BspANI GGCC 7 cut(s) 125, 219, 317, 1152, 2649, 2702, 2843
BspCNI CTCAG 8 cut(s) 25, 1171, 1321, 1915, 1995, 2626, 2758, 2839
BspDI ATCGAT 1 cut(s) 2116
BspHI TCATGA 1 cut(s) 1495
BspMAI CTGCAG 3 cut(s) 77, 380, 1062
BspMI ACCTGC 2 cut(s) 80, 911
BspPI GGATC 4 cut(s) 286, 580, 850, 2677
BspQI GCTCTTC 1 cut(s) 1934
BspT107I GGYRCC 3 cut(s) 453, 951, 2072
BspTNI GGTCTC 1 cut(s) 512
BsrDI GCAATG 1 cut(s) 1681
BsrFI RCCGGY 1 cut(s) 1586
BsrGI TGTACA 2 cut(s) 265, 945
BsrI ACTGG 7 cut(s) 318, 527, 1317, 1765, 2287, 2563, 2822
BssAI RCCGGY 1 cut(s) 1586
BssECI CCNNGG 7 cut(s) 26, 291, 955, 1218, 2521, 2673, 2674
BssT1I CCWWGG 4 cut(s) 26, 955, 1218, 2521
Bst2UI CCWGG 5 cut(s) 122, 1131, 1812, 2207, 2675
Bst4CI ACNGT 5 cut(s) 597, 796, 1468, 2128, 2806
Bst6I CTCTTC 3 cut(s) 1698, 1934, 2083
BstAPI GCANNNNNTGC 2 cut(s) 72, 375
BstAUI TGTACA 2 cut(s) 265, 945
BstC8I GCNNGC 4 cut(s) 371, 613, 1171, 2110
BstDSI CCRYGG 3 cut(s) 26, 955, 1218
BstH2I RGCGCY 1 cut(s) 868
BstHHI GCGC 2 cut(s) 867, 872
BstMAI GTCTC 1 cut(s) 512
BstMCI CGRYCG 1 cut(s) 59
BstMWI GCNNNNNNNGC 6 cut(s) 72, 375, 551, 811, 856, 2479
BstNI CCWGG 5 cut(s) 122, 1131, 1812, 2207, 2675
BstNSI RCATGY 3 cut(s) 788, 1573, 2837
BstSCI CCNGG 5 cut(s) 120, 1129, 1810, 2205, 2673
BstSFI CTRYAG 5 cut(s) 73, 324, 376, 795, 1058
BstSLI GKGCMC 1 cut(s) 2811
BstV1I GCAGC 6 cut(s) 390, 554, 789, 854, 1939, 2460
BstV2I GAAGAC 2 cut(s) 42, 1763
BstX2I RGATCY 3 cut(s) 278, 585, 2682
BstXI CCANNNNNNTGG 1 cut(s) 2710
BstYI RGATCY 3 cut(s) 278, 585, 2682
Bsu15I ATCGAT 1 cut(s) 2116
Bsu36I CCTNAGG 2 cut(s) 2724, 2766
BsuI GTATCC 1 cut(s) 289
BsuRI GGCC 7 cut(s) 125, 219, 317, 1152, 2649, 2702, 2843
BsuTUI ATCGAT 1 cut(s) 2116
BtgI CCRYGG 3 cut(s) 26, 955, 1218
BtrI CACGTC 1 cut(s) 679
BtsIMutI CAGTG 6 cut(s) 325, 1464, 2133, 2294, 2802, 2829
BveI ACCTGC 2 cut(s) 80, 911
Cac8I GCNNGC 4 cut(s) 371, 613, 1171, 2110
CaiI CAGNNNCTG 3 cut(s) 375, 533, 2558
CciI TCATGA 1 cut(s) 1495
CfoI GCGC 2 cut(s) 867, 872
Cfr10I RCCGGY 1 cut(s) 1586
Cfr13I GGNCC 7 cut(s) 7, 118, 287, 403, 1257, 2647, 2841
ClaI ATCGAT 1 cut(s) 2116
Csp6I GTAC 4 cut(s) 40, 266, 900, 946
CspAI ACCGGT 1 cut(s) 1586
CspCI CAANNNNNGTGG 2 cut(s) 2487, 2522
CviQI GTAC 4 cut(s) 40, 266, 900, 946
DraI TTTAAA 4 cut(s) 393, 1072, 1396, 1738
DriI GACNNNNNGTC 1 cut(s) 2176
EaeI YGGCCR 1 cut(s) 1150
Eam1104I CTCTTC 3 cut(s) 1698, 1934, 2083
Eam1105I GACNNNNNGTC 1 cut(s) 2176
EarI CTCTTC 3 cut(s) 1698, 1934, 2083
EciI GGCGGA 1 cut(s) 20
Ecl136II GAGCTC 2 cut(s) 226, 2692
Eco130I CCWWGG 4 cut(s) 26, 955, 1218, 2521
Eco147I AGGCCT 1 cut(s) 219
Eco24I GRGCYC 3 cut(s) 228, 763, 2694
Eco31I GGTCTC 1 cut(s) 512
Eco47I GGWCC 5 cut(s) 7, 118, 287, 403, 1257
Eco47III AGCGCT 1 cut(s) 866
Eco53kI GAGCTC 2 cut(s) 226, 2692
Eco57I CTGAAG 2 cut(s) 444, 2565
Eco81I CCTNAGG 2 cut(s) 2724, 2766
Eco88I CYCGRG 2 cut(s) 221, 357
EcoICRI GAGCTC 2 cut(s) 226, 2692
EcoO109I RGGNCCY 1 cut(s) 2841
EcoRI GAATTC 1 cut(s) 2426
EcoRII CCWGG 5 cut(s) 120, 1129, 1810, 2205, 2673
EcoT14I CCWWGG 4 cut(s) 26, 955, 1218, 2521
EcoT38I GRGCYC 3 cut(s) 228, 763, 2694
ErhI CCWWGG 4 cut(s) 26, 955, 1218, 2521
FalI AAGNNNNNCTT 2 cut(s) 1913, 1945
FaqI GGGAC 2 cut(s) 1677, 2760
FbaI TGATCA 2 cut(s) 535, 643
Fnu4HI GCNGC 6 cut(s) 379, 543, 803, 868, 1953, 2474
FriOI GRGCYC 3 cut(s) 228, 763, 2694
Fsp4HI GCNGC 6 cut(s) 379, 543, 803, 868, 1953, 2474
FspBI CTAG 5 cut(s) 165, 438, 1455, 1700, 2195
GlaI GCGC 2 cut(s) 866, 871
GluI GCNGC 6 cut(s) 379, 543, 803, 868, 1953, 2474
GsaI CCCAGC 2 cut(s) 1257, 1890
GsuI CTGGAG 5 cut(s) 957, 1152, 2189, 2270, 2535
HaeII RGCGCY 1 cut(s) 868
HaeIII GGCC 7 cut(s) 125, 219, 317, 1152, 2649, 2702, 2843
HapII CCGG 3 cut(s) 232, 1587, 2449
HhaI GCGC 2 cut(s) 867, 872
Hin6I GCGC 2 cut(s) 865, 870
HinP1I GCGC 2 cut(s) 865, 870
HincII GTYRAC 4 cut(s) 600, 684, 1584, 1849
HindII GTYRAC 4 cut(s) 600, 684, 1584, 1849
HindIII AAGCTT 3 cut(s) 1140, 1171, 1409
HpaI GTTAAC 1 cut(s) 1849
HpaII CCGG 3 cut(s) 232, 1587, 2449
HphI GGTGA 5 cut(s) 474, 653, 686, 1457, 2926
Hpy166II GTNNAC 6 cut(s) 287, 600, 684, 1584, 1849, 2496
Hpy8I GTNNAC 6 cut(s) 287, 600, 684, 1584, 1849, 2496
Hpy99I CGWCG 2 cut(s) 140, 1014
HpyCH4III ACNGT 5 cut(s) 597, 796, 1468, 2128, 2806
HpyCH4IV ACGT 4 cut(s) 678, 1009, 1741, 1935
HpyF10VI GCNNNNNNNGC 6 cut(s) 72, 375, 551, 811, 856, 2479
HpySE526I ACGT 4 cut(s) 678, 1009, 1741, 1935
HspAI GCGC 2 cut(s) 865, 870
Ksp22I TGATCA 2 cut(s) 535, 643
KspAI GTTAAC 1 cut(s) 1849
LguI GCTCTTC 1 cut(s) 1934
LmnI GCTCC 9 cut(s) 231, 434, 890, 938, 975, 2289, 2446, 2542, 2554
Lsp1109I GCAGC 6 cut(s) 390, 554, 789, 854, 1939, 2460
LweI GCATC 7 cut(s) 1315, 1711, 1914, 1977, 2095, 2233, 2886
MaeI CTAG 5 cut(s) 165, 438, 1455, 1700, 2195
MaeII ACGT 4 cut(s) 678, 1009, 1741, 1935
MaeIII GTNAC 5 cut(s) 674, 787, 1462, 1523, 2800
MfeI CAATTG 1 cut(s) 1362
MflI RGATCY 3 cut(s) 278, 585, 2682
MhlI GDGCHC 6 cut(s) 228, 763, 895, 2614, 2694, 2811
MlsI TGGCCA 1 cut(s) 1152
MluNI TGGCCA 1 cut(s) 1152
MlyI GAGTC 3 cut(s) 2252, 2392, 2780
MmeI TCCRAC 2 cut(s) 648, 2754
Mox20I TGGCCA 1 cut(s) 1152
MscI TGGCCA 1 cut(s) 1152
MslI CAYNNNNRTG 3 cut(s) 149, 2316, 2708
Msp20I TGGCCA 1 cut(s) 1152
MspA1I CMGCKG 1 cut(s) 545
MspI CCGG 3 cut(s) 232, 1587, 2449
MspR9I CCNGG 5 cut(s) 122, 1131, 1812, 2207, 2675
MteI GCGCNGCGC 1 cut(s) 868
MunI CAATTG 1 cut(s) 1362
Mva1269I GAATGC 1 cut(s) 2475
MvaI CCWGG 5 cut(s) 122, 1131, 1812, 2207, 2675
MwoI GCNNNNNNNGC 6 cut(s) 72, 375, 551, 811, 856, 2479
NcoI CCATGG 3 cut(s) 26, 955, 1218
NmuCI GTSAC 5 cut(s) 674, 787, 1462, 1523, 2800
NspI RCATGY 3 cut(s) 788, 1573, 2837
OliI CACNNNNGTG 1 cut(s) 2316
PaeR7I CTCGAG 1 cut(s) 221
PagI TCATGA 1 cut(s) 1495
PasI CCCWGGG 1 cut(s) 2674
PceI AGGCCT 1 cut(s) 219
PciI ACATGT 1 cut(s) 2833
PciSI GCTCTTC 1 cut(s) 1934
PctI GAATGC 1 cut(s) 2475
PfeI GAWTC 7 cut(s) 476, 833, 1214, 1499, 2053, 2299, 2828
PflFI GACNNNGTC 1 cut(s) 2597
PflMI CCANNNNNTGG 1 cut(s) 2558
PfoI TCCNGGA 2 cut(s) 1129, 2205
PinAI ACCGGT 1 cut(s) 1586
PkrI GCNGC 6 cut(s) 380, 544, 804, 869, 1954, 2475
Ple19I CGATCG 1 cut(s) 59
PleI GAGTC 3 cut(s) 2252, 2391, 2779
PpsI GAGTC 3 cut(s) 2252, 2391, 2779
PscI ACATGT 1 cut(s) 2833
Psp124BI GAGCTC 2 cut(s) 228, 2694
Psp1406I AACGTT 2 cut(s) 1741, 1935
Psp6I CCWGG 5 cut(s) 120, 1129, 1810, 2205, 2673
PspFI CCCAGC 2 cut(s) 1253, 1886
PspGI CCWGG 5 cut(s) 120, 1129, 1810, 2205, 2673
PspPI GGNCC 7 cut(s) 7, 118, 287, 403, 1257, 2647, 2841
PspXI VCTCGAGB 1 cut(s) 221
PstI CTGCAG 3 cut(s) 77, 380, 1062
PstNI CAGNNNCTG 3 cut(s) 375, 533, 2558
PsuI RGATCY 3 cut(s) 278, 585, 2682
PsyI GACNNNGTC 1 cut(s) 2597
PvuI CGATCG 1 cut(s) 59
PvuII CAGCTG 1 cut(s) 545
RsaI GTAC 4 cut(s) 41, 267, 901, 947
RsaNI GTAC 4 cut(s) 40, 266, 900, 946
RseI CAYNNNNRTG 3 cut(s) 149, 2316, 2708
SacI GAGCTC 2 cut(s) 228, 2694
SapI GCTCTTC 1 cut(s) 1934
SatI GCNGC 6 cut(s) 379, 543, 803, 868, 1953, 2474
Sau96I GGNCC 7 cut(s) 7, 118, 287, 403, 1257, 2647, 2841
SchI GAGTC 3 cut(s) 2252, 2392, 2780
ScrFI CCNGG 5 cut(s) 122, 1131, 1812, 2207, 2675
SduI GDGCHC 6 cut(s) 228, 763, 895, 2614, 2694, 2811
SfaNI GCATC 7 cut(s) 1315, 1711, 1914, 1977, 2095, 2233, 2886
SfcI CTRYAG 5 cut(s) 73, 324, 376, 795, 1058
Sfr274I CTCGAG 1 cut(s) 221
SinI GGWCC 5 cut(s) 7, 118, 287, 403, 1257
SlaI CTCGAG 1 cut(s) 221
SmiMI CAYNNNNRTG 3 cut(s) 149, 2316, 2708
SmlI CTYRAG 7 cut(s) 221, 828, 996, 1532, 1751, 2693, 2886
SmoI CTYRAG 7 cut(s) 221, 828, 996, 1532, 1751, 2693, 2886
SseBI AGGCCT 1 cut(s) 219
SsiI CCGC 4 cut(s) 5, 103, 1112, 2147
SspMI CTAG 5 cut(s) 165, 438, 1455, 1700, 2195
SstI GAGCTC 2 cut(s) 228, 2694
StuI AGGCCT 1 cut(s) 219
StyD4I CCNGG 5 cut(s) 120, 1129, 1810, 2205, 2673
StyI CCWWGG 4 cut(s) 26, 955, 1218, 2521
TaaI ACNGT 5 cut(s) 597, 796, 1468, 2128, 2806
TaiI ACGT 4 cut(s) 681, 1012, 1744, 1938
TaqI TCGA 5 cut(s) 36, 135, 222, 2116, 2430
TatI WGTACW 2 cut(s) 265, 945
TfiI GAWTC 7 cut(s) 476, 833, 1214, 1499, 2053, 2299, 2828
TscAI CASTG 6 cut(s) 325, 1471, 2133, 2294, 2809, 2829
TseFI GTSAC 5 cut(s) 674, 787, 1462, 1523, 2800
TseI GCWGC 6 cut(s) 378, 542, 802, 867, 1952, 2473
Tsp45I GTSAC 5 cut(s) 674, 787, 1462, 1523, 2800
TspGWI ACGGA 5 cut(s) 395, 1249, 1643, 2057, 2390
TspRI CASTG 6 cut(s) 325, 1471, 2133, 2294, 2809, 2829
Tth111I GACNNNGTC 1 cut(s) 2597
Van91I CCANNNNNTGG 1 cut(s) 2558
VpaK11BI GGWCC 5 cut(s) 7, 118, 287, 403, 1257
XapI RAATTY 3 cut(s) 1817, 1964, 2426
XceI RCATGY 3 cut(s) 788, 1573, 2837
XcmI CCANNNNNNNNNTGG 2 cut(s) 469, 1160
XhoI CTCGAG 1 cut(s) 221
XspI CTAG 5 cut(s) 165, 438, 1455, 1700, 2195
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.