AT5G57050

phosphatase 2C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0010053
Physical Location & Seq
Forward (+)
23087122 .. 23089631
2510 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G57050.2

Sequence Viewer

Length: 1272 bp
ATGGACGAAGTTTCTCCTGCAGTCGCTGTTCCATTCAGACCATTCACTGACCCTCACGCCGGACTTAGAGGCTATTGCAACGGTGAATCTAGGGTTACTTTACCGGAAAGTTCTTGTTCTGGCGACGGAGCTATGAAAGATTCTTCCTTTGAGATCAATACAAGACAAGATTCATTGACATCATCATCATCTGCTATGGCAGGTGTGGATATCTCCGCCGGAGATGAAATCAACGGTTCAGATGAGTTTGATCCGAGATCGATGAATCAGAGTGAGAAGAAAGTACTTAGTAGAACAGAGAGTAGAAGTCTGTTTGAGTTCAAGTGTGTTCCTTTATATGGAGTGACTTCGATTTGTGGTAGACGACCAGAGATGGAAGATTCTGTCTCAACGATTCCTAGATTCCTTCAAGTTTCTTCTAGTTCGTTGCTTGATGGTCGAGTCACTAATGGATTTAATCCTCACTTGAGTGCTCATTTCTTTGGTGTTTACGATGGCCATGGCGGTTCTCAGGTAGCGAATTATTGTCGTGAGAGGATGCATCTGGCTTTGACGGAGGAGATAGTGAAGGAGAAACCGGAGTTTTGTGACGGTGACACGTGGCAAGAGAAGTGGAAGAAGGCTTTGTTCAACTCTTTTATGAGAGTTGACTCGGAGATTGAAACTGTGGCTCATGCTCCGGAAACTGTTGGGTCTACCTCGGTGGTTGCGGTTGTCTTTCCGACTCACATCTTTGTCGCGAATTGCGGCGACTCTAGGGCGGTTTTGTGTCGCGGCAAAACGCCACTCGCGTTGTCGGTTGATCACAAACCGGATAGGGATGATGAAGCGGCGAGGATAGAAGCTGCCGGTGGGAAAGTAATCCGGTGGAACGGGGCTCGTGTATTTGGTGTTCTCGCAATGTCAAGATCCATTGGCGATAGATACCTTAAACCGTCAGTAATTCCGGATCCAGAAGTGACTTCAGTGCGGCGAGTAAAAGAAGATGATTGTCTCATCTTAGCAAGTGATGGTCTTTGGGATGTAATGACAAACGAAGAAGTGTGCGATTTGGCTCGGAAACGGATTTTACTATGGCATAAGAAGAACGCGATGGCCGGAGAGGCTTTGCTTCCGGCGGAGAAAAGAGGAGAAGGAAAAGATCCTGCAGCAATGTCCGCGGCAGAGTATTTGTCGAAGATGGCTTTGCAAAAAGGAAGCAAAGACAATATAAGTGTGGTAGTGGTTGATTTGAAGGGAATAAGGAAATTCAAGAGCAAATCCTTGAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0001932 GO:0001933 GO:0003674 GO:0003824 GO:0004721 GO:0004722 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005886 GO:0006464 GO:0006469 GO:0006470 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007275 GO:0008150 GO:0008152 GO:0009266 GO:0009314 GO:0009408 GO:0009409 GO:0009414 GO:0009415 GO:0009416 GO:0009628 GO:0009642 GO:0009644 GO:0009719 GO:0009725 GO:0009737 GO:0009787 GO:0009788 GO:0009892 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010035 GO:0010109 GO:0010119 GO:0010205 GO:0010563 GO:0010605 GO:0010646 GO:0010648 GO:0016020 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0019220 GO:0019222 GO:0019538 GO:0019899 GO:0019900 GO:0019901 GO:0022622 GO:0023051 GO:0023057 GO:0031323 GO:0031324 GO:0031399 GO:0031400 GO:0032268 GO:0032269 GO:0032501 GO:0032502 GO:0033673 GO:0033993 GO:0036211 GO:0042221 GO:0042325 GO:0042326 GO:0042548 GO:0042578 GO:0043086 GO:0043155 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043467 GO:0043549 GO:0044092 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045859 GO:0045936 GO:0048364 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0048731 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051174 GO:0051246 GO:0051248 GO:0051338 GO:0051348 GO:0060255 GO:0065007 GO:0065009 GO:0071704 GO:0071944 GO:0080090 GO:0097305 GO:0099402 GO:0140096 GO:1901419 GO:1901420 GO:1901564 GO:1901700 GO:1902456 GO:1905156 GO:1905957 GO:1905958
KEGG Pathways
Metabolic & Signaling
ko04016 ko04075 ko04931 map04016 map04075 map04931
Pfam Domains
Protein Families
PP2C

Protein Analysis

423

Amino Acids

46.31

Weight (kDa)

5.93

Isoelectric Point (pI)

50.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 90 - 364 1.2e-70 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 191
Acc36I ACCTGC 1 cut(s) 191
AccI GTMKAC 2 cut(s) 361, 695
AccII CGCG 5 cut(s) 740, 774, 791, 1091, 1160
AccIII TCCGGA 2 cut(s) 679, 946
AclWI GGATC 5 cut(s) 245, 903, 944, 957, 1136
AcoI YGGCCR 2 cut(s) 496, 1095
AcsI RAATTY 1 cut(s) 1247
AcuI CTGAAG 1 cut(s) 948
AcvI CACGTG 1 cut(s) 600
AfaI GTAC 1 cut(s) 285
AfiI CCNNNNNNNGG 2 cut(s) 59, 338
AflIII ACRYGT 1 cut(s) 597
AgsI TTSAA 7 cut(s) 322, 410, 631, 662, 1234, 1252, 1267
AleI CACNNNNGTG 1 cut(s) 468
AluBI AGCT 2 cut(s) 131, 845
AluI AGCT 2 cut(s) 131, 845
Alw21I GWGCWC 1 cut(s) 475
Alw26I GTCTC 2 cut(s) 391, 998
AlwI GGATC 5 cut(s) 245, 903, 944, 957, 1136
AlwNI CAGNNNCTG 1 cut(s) 26
Aor13HI TCCGGA 2 cut(s) 679, 946
AoxI GGCC 2 cut(s) 496, 1095
ApeKI GCWGC 2 cut(s) 845, 1148
ApoI RAATTY 1 cut(s) 1247
AsuHPI GGTGA 2 cut(s) 95, 605
BalI TGGCCA 1 cut(s) 498
BamHI GGATCC 1 cut(s) 949
BanII GRGCYC 1 cut(s) 880
BauI CACGAG 1 cut(s) 879
BbrPI CACGTG 1 cut(s) 600
Bbv12I GWGCWC 1 cut(s) 475
BbvI GCAGC 2 cut(s) 832, 1160
BccI CCATC 6 cut(s) 367, 428, 488, 1004, 1087, 1174
BclI TGATCA 1 cut(s) 802
BcoDI GTCTC 2 cut(s) 391, 998
BfaI CTAG 4 cut(s) 90, 399, 420, 756
BfmI CTRYAG 2 cut(s) 18, 1146
BfuAI ACCTGC 1 cut(s) 191
BglI GCCNNNNNGGC 1 cut(s) 1103
BisI GCNGC 7 cut(s) 748, 775, 831, 846, 971, 1149, 1161
BlsI GCNGC 7 cut(s) 749, 776, 832, 847, 972, 1150, 1162
BmcAI AGTACT 1 cut(s) 285
BmiI GGNNCC 1 cut(s) 951
BmsI GCATC 2 cut(s) 528, 550
BpuEI CTTGAG 1 cut(s) 487
Bsa29I ATCGAT 1 cut(s) 260
BsaAI YACGTR 1 cut(s) 600
BsaJI CCNNGG 3 cut(s) 499, 699, 1158
BsaWI WCCGGW 6 cut(s) 103, 577, 679, 811, 864, 946
Bsc4I CCNNNNNNNGG 2 cut(s) 59, 338
Bse118I RCCGGY 1 cut(s) 848
Bse3DI GCAATG 2 cut(s) 906, 1158
BseAI TCCGGA 2 cut(s) 679, 946
BseCI ATCGAT 1 cut(s) 260
BseDI CCNNGG 3 cut(s) 499, 699, 1158
BseGI GGATG 3 cut(s) 543, 826, 1027
BseLI CCNNNNNNNGG 2 cut(s) 59, 338
BseMI GCAATG 2 cut(s) 906, 1158
BseMII CTCAG 1 cut(s) 524
BseRI GAGGAG 2 cut(s) 572, 1143
BseXI GCAGC 2 cut(s) 832, 1160
Bsh1236I CGCG 5 cut(s) 740, 774, 791, 1091, 1160
BshFI GGCC 2 cut(s) 498, 1097
BshVI ATCGAT 1 cut(s) 260
BsiHKAI GWGCWC 1 cut(s) 475
BslI CCNNNNNNNGG 2 cut(s) 59, 338
BsmAI GTCTC 2 cut(s) 391, 998
BsnI GGCC 2 cut(s) 498, 1097
Bsp1286I GDGCHC 2 cut(s) 475, 880
Bsp13I TCCGGA 2 cut(s) 679, 946
Bsp143I GATC 7 cut(s) 153, 250, 257, 802, 908, 949, 1141
Bsp19I CCATGG 1 cut(s) 499
Bsp68I TCGCGA 1 cut(s) 740
BspANI GGCC 2 cut(s) 498, 1097
BspCNI CTCAG 1 cut(s) 523
BspDI ATCGAT 1 cut(s) 260
BspEI TCCGGA 2 cut(s) 679, 946
BspFNI CGCG 5 cut(s) 740, 774, 791, 1091, 1160
BspLI GGNNCC 1 cut(s) 951
BspMAI CTGCAG 2 cut(s) 22, 1150
BspMI ACCTGC 1 cut(s) 191
BspPI GGATC 5 cut(s) 245, 903, 944, 957, 1136
BsrDI GCAATG 2 cut(s) 906, 1158
BsrFI RCCGGY 1 cut(s) 848
BssAI RCCGGY 1 cut(s) 848
BssECI CCNNGG 3 cut(s) 499, 699, 1158
BssMI GATC 7 cut(s) 153, 250, 257, 802, 908, 949, 1141
BssSI CACGAG 1 cut(s) 879
BssT1I CCWWGG 1 cut(s) 499
Bst2BI CACGAG 1 cut(s) 879
Bst4CI ACNGT 6 cut(s) 83, 236, 593, 667, 688, 936
BstBAI YACGTR 1 cut(s) 600
BstDEI CTNAG 4 cut(s) 65, 287, 510, 1000
BstDSI CCRYGG 2 cut(s) 499, 1158
BstF5I GGATG 3 cut(s) 543, 826, 1027
BstFNI CGCG 5 cut(s) 740, 774, 791, 1091, 1160
BstKTI GATC 7 cut(s) 156, 253, 260, 805, 911, 952, 1144
BstMAI GTCTC 2 cut(s) 391, 998
BstMBI GATC 7 cut(s) 153, 250, 257, 802, 908, 949, 1141
BstMWI GCNNNNNNNGC 2 cut(s) 1103, 1157
BstSFI CTRYAG 2 cut(s) 18, 1146
BstUI CGCG 5 cut(s) 740, 774, 791, 1091, 1160
BstV1I GCAGC 2 cut(s) 832, 1160
BstX2I RGATCY 3 cut(s) 908, 949, 1141
BstYI RGATCY 3 cut(s) 908, 949, 1141
Bsu15I ATCGAT 1 cut(s) 260
BsuRI GGCC 2 cut(s) 498, 1097
BsuTUI ATCGAT 1 cut(s) 260
BtgI CCRYGG 2 cut(s) 499, 1158
BtgZI GCGATG 1 cut(s) 1106
BtsCI GGATG 3 cut(s) 543, 826, 1027
BtsIMutI CAGTG 2 cut(s) 45, 972
BtuMI TCGCGA 1 cut(s) 740
BveI ACCTGC 1 cut(s) 191
CaiI CAGNNNCTG 1 cut(s) 26
Cfr10I RCCGGY 1 cut(s) 848
Cfr42I CCGCGG 1 cut(s) 1161
ClaI ATCGAT 1 cut(s) 260
Csp6I GTAC 1 cut(s) 284
CspCI CAANNNNNGTGG 4 cut(s) 593, 628, 774, 809
CviAII CATG 2 cut(s) 500, 674
CviQI GTAC 1 cut(s) 284
DdeI CTNAG 4 cut(s) 65, 287, 510, 1000
DpnI GATC 7 cut(s) 155, 252, 259, 804, 910, 951, 1143
DpnII GATC 7 cut(s) 153, 250, 257, 802, 908, 949, 1141
EaeI YGGCCR 2 cut(s) 496, 1095
EciI GGCGGA 2 cut(s) 205, 1133
Eco130I CCWWGG 1 cut(s) 499
Eco24I GRGCYC 1 cut(s) 880
Eco32I GATATC 1 cut(s) 211
Eco57I CTGAAG 1 cut(s) 948
Eco72I CACGTG 1 cut(s) 600
EcoRV GATATC 1 cut(s) 211
EcoT14I CCWWGG 1 cut(s) 499
EcoT22I ATGCAT 1 cut(s) 543
EcoT38I GRGCYC 1 cut(s) 880
ErhI CCWWGG 1 cut(s) 499
FaeI CATG 2 cut(s) 503, 677
FatI CATG 2 cut(s) 499, 673
FbaI TGATCA 1 cut(s) 802
FblI GTMKAC 2 cut(s) 361, 695
Fnu4HI GCNGC 7 cut(s) 748, 775, 831, 846, 971, 1149, 1161
FokI GGATG 3 cut(s) 550, 833, 1034
FriOI GRGCYC 1 cut(s) 880
Fsp4HI GCNGC 7 cut(s) 748, 775, 831, 846, 971, 1149, 1161
FspBI CTAG 4 cut(s) 90, 399, 420, 756
GluI GCNGC 7 cut(s) 748, 775, 831, 846, 971, 1149, 1161
HaeIII GGCC 2 cut(s) 498, 1097
Hin1II CATG 2 cut(s) 503, 677
HincII GTYRAC 1 cut(s) 649
HindII GTYRAC 1 cut(s) 649
HphI GGTGA 2 cut(s) 95, 605
Hpy166II GTNNAC 4 cut(s) 362, 490, 649, 696
Hpy188I TCNGA 7 cut(s) 38, 241, 255, 270, 655, 723, 1059
Hpy188III TCNNGA 7 cut(s) 530, 680, 739, 906, 947, 953, 1252
Hpy8I GTNNAC 4 cut(s) 362, 490, 649, 696
Hpy99I CGWCG 1 cut(s) 128
HpyAV CCTTC 5 cut(s) 416, 562, 613, 1127, 1228
HpyCH4III ACNGT 6 cut(s) 83, 236, 593, 667, 688, 936
HpyCH4IV ACGT 1 cut(s) 599
HpyCH4V TGCA 5 cut(s) 20, 78, 541, 1148, 1189
HpyF10VI GCNNNNNNNGC 2 cut(s) 1103, 1157
HpyF3I CTNAG 4 cut(s) 65, 287, 510, 1000
HpySE526I ACGT 1 cut(s) 599
Hsp92II CATG 2 cut(s) 503, 677
Kpn2I TCCGGA 2 cut(s) 679, 946
Ksp22I TGATCA 1 cut(s) 802
KspI CCGCGG 1 cut(s) 1161
Kzo9I GATC 7 cut(s) 153, 250, 257, 802, 908, 949, 1141
LmnI GCTCC 2 cut(s) 128, 682
Lsp1109I GCAGC 2 cut(s) 832, 1160
LweI GCATC 2 cut(s) 528, 550
MaeI CTAG 4 cut(s) 90, 399, 420, 756
MaeII ACGT 1 cut(s) 599
MaeIII GTNAC 6 cut(s) 94, 343, 442, 587, 593, 958
MalI GATC 7 cut(s) 155, 252, 259, 804, 910, 951, 1143
MboI GATC 7 cut(s) 153, 250, 257, 802, 908, 949, 1141
MboII GAAGA 9 cut(s) 135, 289, 389, 408, 628, 995, 1049, 1096, 1189
MflI RGATCY 3 cut(s) 908, 949, 1141
MhlI GDGCHC 2 cut(s) 475, 880
MlsI TGGCCA 1 cut(s) 498
MluCI AATT 5 cut(s) 520, 742, 942, 1247, 1267
MluNI TGGCCA 1 cut(s) 498
MlyI GAGTC 4 cut(s) 450, 644, 718, 746
MmeI TCCRAC 1 cut(s) 746
MnlI CCTC 9 cut(s) 62, 63, 471, 528, 550, 709, 828, 1096, 1121
Mox20I TGGCCA 1 cut(s) 498
Mph1103I ATGCAT 1 cut(s) 543
MroI TCCGGA 2 cut(s) 679, 946
MscI TGGCCA 1 cut(s) 498
MseI TTAA 2 cut(s) 456, 930
MslI CAYNNNNRTG 1 cut(s) 468
Msp20I TGGCCA 1 cut(s) 498
MspA1I CMGCKG 1 cut(s) 1160
MvnI CGCG 5 cut(s) 740, 774, 791, 1091, 1160
MwoI GCNNNNNNNGC 2 cut(s) 1103, 1157
NcoI CCATGG 1 cut(s) 499
NdeII GATC 7 cut(s) 153, 250, 257, 802, 908, 949, 1141
NlaIII CATG 2 cut(s) 503, 677
NlaIV GGNNCC 1 cut(s) 951
NmuCI GTSAC 5 cut(s) 343, 442, 587, 593, 958
NruI TCGCGA 1 cut(s) 740
NsiI ATGCAT 1 cut(s) 543
OliI CACNNNNGTG 1 cut(s) 468
PaqCI CACCTGC 1 cut(s) 191
PcsI WCGNNNNNNNCGW 1 cut(s) 788
PfeI GAWTC 7 cut(s) 86, 140, 170, 265, 380, 394, 402
PkrI GCNGC 7 cut(s) 749, 776, 832, 847, 972, 1150, 1162
PleI GAGTC 4 cut(s) 449, 644, 718, 746
PmaCI CACGTG 1 cut(s) 600
PmlI CACGTG 1 cut(s) 600
PpsI GAGTC 4 cut(s) 449, 644, 718, 746
Ppu21I YACGTR 1 cut(s) 600
PspCI CACGTG 1 cut(s) 600
PspN4I GGNNCC 1 cut(s) 951
PsrI GAACNNNNNNTAC 2 cut(s) 876, 908
PstI CTGCAG 2 cut(s) 22, 1150
PstNI CAGNNNCTG 1 cut(s) 26
PsuI RGATCY 3 cut(s) 908, 949, 1141
RruI TCGCGA 1 cut(s) 740
RsaI GTAC 1 cut(s) 285
RsaNI GTAC 1 cut(s) 284
RseI CAYNNNNRTG 1 cut(s) 468
SacII CCGCGG 1 cut(s) 1161
SaqAI TTAA 2 cut(s) 456, 930
SatI GCNGC 7 cut(s) 748, 775, 831, 846, 971, 1149, 1161
Sau3AI GATC 7 cut(s) 153, 250, 257, 802, 908, 949, 1141
ScaI AGTACT 1 cut(s) 285
SchI GAGTC 4 cut(s) 450, 644, 718, 746
SduI GDGCHC 2 cut(s) 475, 880
SetI ASST 7 cut(s) 133, 205, 516, 602, 701, 847, 930
SfaNI GCATC 2 cut(s) 528, 550
SfcI CTRYAG 2 cut(s) 18, 1146
Sfr303I CCGCGG 1 cut(s) 1161
SgrBI CCGCGG 1 cut(s) 1161
SmiMI CAYNNNNRTG 1 cut(s) 468
SmlI CTYRAG 1 cut(s) 466
SmoI CTYRAG 1 cut(s) 466
Sse9I AATT 5 cut(s) 520, 742, 942, 1247, 1267
SspMI CTAG 4 cut(s) 90, 399, 420, 756
StyI CCWWGG 1 cut(s) 499
TaaI ACNGT 6 cut(s) 83, 236, 593, 667, 688, 936
TaiI ACGT 1 cut(s) 602
TaqI TCGA 4 cut(s) 260, 350, 439, 1175
TasI AATT 5 cut(s) 520, 742, 942, 1247, 1267
TatI WGTACW 1 cut(s) 283
TauI GCSGC 5 cut(s) 750, 777, 833, 973, 1163
TfiI GAWTC 7 cut(s) 86, 140, 170, 265, 380, 394, 402
Tru1I TTAA 2 cut(s) 456, 930
Tru9I TTAA 2 cut(s) 456, 930
TscAI CASTG 2 cut(s) 52, 972
TseFI GTSAC 5 cut(s) 343, 442, 587, 593, 958
TseI GCWGC 2 cut(s) 845, 1148
Tsp45I GTSAC 5 cut(s) 343, 442, 587, 593, 958
TspDTI ATGAA 5 cut(s) 149, 162, 240, 278, 840
TspGWI ACGGA 3 cut(s) 141, 569, 1078
TspRI CASTG 2 cut(s) 52, 972
XapI RAATTY 1 cut(s) 1247
XmiI GTMKAC 2 cut(s) 361, 695
XspI CTAG 4 cut(s) 90, 399, 420, 756
ZrmI AGTACT 1 cut(s) 285
Zsp2I ATGCAT 1 cut(s) 543
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.