MD15G1212000.v1.1

phosphatase 2C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0010053
Physical Location & Seq
Forward (+)
17029685 .. 17032017
2333 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1212000.v1.1.491

Sequence Viewer

Length: 1341 bp
ATGGACGATTTAGCTCAGGCAATTGCTGTTCCATACAGGCTAGGTAATTTGATCCATGATGAGTCAGCAGTAAAATCCCAGATGGAAATTACTGGCCTCAAACTAATAGCAAATGCTTCACCCTTGTTATTTAATAGTCCTCATGATGAAAAACGGGGAGTGGAAGTCGTGAATCGGGGAAGTCAGGAATATAATAAGTTCAGGCCAACGTGTGATCGCGTTGGCGATGGATCGAGTACTTGTGCTGCATCCACCTCGCTTTTTTCGGCCACTGGGGATGAGAAAATGTGCAGAACAAGCTGCAGTCGGAGAGTTTTGGATTTGGATCGTGTACCCCTTTGGGGCTACACGTCTATCATTGGAAGGAGACCGGAGATGGAAGATGACGTTGCAGTTGTACCACGATTTTCCCAGGTTCCGGTTCAAATGCTGATGGATGACAGTGTCTTGAATGGCATGAATCAAAACACAAGTGACTTAACTGCTCATTTTTTTGGAGTATATGATGGACATGGAGGTTCTCAGGTTGCTAATTATTGTCGCGAACGCATGCATTCTGCATTGGCCGAAGAGCTAGAAGTCGCGAAAACAGGCTTAGATGACCGAAGCAATGCGGAGGAGGGTTGGCAGGAGAGGTGGAAGGAGGCCTTCTCCAATAGTTTCTTGAAAGTGGATGCTGAGATTGGAGGAGCTCCAAGAGGCACAGATGTGAGCAAAACTGATGCTTCTTCTGGGGGAGCTCCAGGAGGCCTTCTCCAACCTATAGCTCCTGAAACCGTCGGATCAACTGCTGTAGTCGCAGTGATTTGTCCAACGCATATTATCGTGTCAAACTGTGGCGATTCGAGAGCAGTATTGTGCAGAGGAAAAGTTCCTGTGCCTTTGTCAGTAGACCACAAGCCAAATAGAGAAGATGAATATGCAAGGATAGAGGCTGCAGGAGGCAAGGTCATACAGTGGAATGGTTCTCGTGTTTGCGGTGTTCTTGCGATGTCAAGGTCAATTGGTGACAGATACATGAAACCATGGATTATACCTGATCCAGAAGTGGTGTTTGTTTCTCGAGAAAAAGAAGACGAGTGCCTAATTCTAGCCAGCGACGGGCTGTGGGATTTCATCACAAACCAGGAGGCCTGCGACATTGCACGAAGGCGGATCCTTCTGTGGCACAAGAAGTACAGTGATACCATGTCAACGGAACGGGGTGAGGGAGCTGATCCTGCTGCCCAATCTGCAGCAGAGTACCTCTCAAAGCTTGCCATGCAAAAGGGGAGCAAGGACAACATCACTGTTGTAGTAGTAGACTTGAAATCCACAAGGAAGTTTAAGAAAAAACCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0001932 GO:0001933 GO:0003674 GO:0003824 GO:0004721 GO:0004722 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005886 GO:0006464 GO:0006469 GO:0006470 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007275 GO:0008150 GO:0008152 GO:0009266 GO:0009314 GO:0009408 GO:0009409 GO:0009414 GO:0009415 GO:0009416 GO:0009628 GO:0009642 GO:0009644 GO:0009719 GO:0009725 GO:0009737 GO:0009787 GO:0009788 GO:0009892 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010035 GO:0010109 GO:0010119 GO:0010205 GO:0010563 GO:0010605 GO:0010646 GO:0010648 GO:0016020 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0019220 GO:0019222 GO:0019538 GO:0019899 GO:0019900 GO:0019901 GO:0022622 GO:0023051 GO:0023057 GO:0031323 GO:0031324 GO:0031399 GO:0031400 GO:0032268 GO:0032269 GO:0032501 GO:0032502 GO:0033673 GO:0033993 GO:0036211 GO:0042221 GO:0042325 GO:0042326 GO:0042548 GO:0042578 GO:0043086 GO:0043155 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043467 GO:0043549 GO:0044092 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045859 GO:0045936 GO:0048364 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0048731 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051174 GO:0051246 GO:0051248 GO:0051338 GO:0051348 GO:0060255 GO:0065007 GO:0065009 GO:0071704 GO:0071944 GO:0080090 GO:0097305 GO:0099402 GO:0140096 GO:1901419 GO:1901420 GO:1901564 GO:1901700 GO:1902456 GO:1905156 GO:1905957 GO:1905958
KEGG Pathways
Metabolic & Signaling
ko04016 ko04075 ko04931 map04016 map04075 map04931
Pfam Domains
Protein Families
PP2C

Protein Analysis

447

Amino Acids

48.82

Weight (kDa)

5.64

Isoelectric Point (pI)

48.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 134 - 429 4.6e-62 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 891, 1302
AccII CGCG 3 cut(s) 219, 543, 584
AciI CCGC 3 cut(s) 614, 978, 1153
AclWI GGATC 8 cut(s) 46, 238, 333, 790, 1034, 1150, 1163, 1211
AcoI YGGCCR 2 cut(s) 267, 564
AfaI GTAC 5 cut(s) 238, 333, 399, 1178, 1244
AfiI CCNNNNNNNGG 3 cut(s) 341, 418, 1101
AflIII ACRYGT 2 cut(s) 209, 348
AgsI TTSAA 4 cut(s) 425, 451, 667, 1309
AjiI CACGTC 1 cut(s) 351
AjnI CCWGG 3 cut(s) 411, 742, 1125
AleI CACNNNNGTG 1 cut(s) 707
AluBI AGCT 8 cut(s) 14, 300, 574, 692, 740, 767, 1214, 1255
AluI AGCT 8 cut(s) 14, 300, 574, 692, 740, 767, 1214, 1255
Alw21I GWGCWC 2 cut(s) 694, 742
Alw26I GTCTC 1 cut(s) 361
AlwI GGATC 8 cut(s) 46, 238, 333, 790, 1034, 1150, 1163, 1211
Ama87I CYCGRG 1 cut(s) 1062
AoxI GGCC 7 cut(s) 94, 203, 267, 564, 645, 748, 1131
ApeKI GCWGC 5 cut(s) 245, 300, 935, 1223, 1235
AsuHPI GGTGA 3 cut(s) 111, 1019, 1217
AvaI CYCGRG 1 cut(s) 1062
BamHI GGATCC 1 cut(s) 1155
BanII GRGCYC 2 cut(s) 694, 742
BauI CACGAG 1 cut(s) 969
BbsI GAAGAC 1 cut(s) 1080
Bbv12I GWGCWC 2 cut(s) 694, 742
BbvI GCAGC 5 cut(s) 232, 287, 922, 1210, 1247
BccI CCATC 5 cut(s) 76, 221, 370, 427, 500
BcgI CGANNNNNNTGC 2 cut(s) 237, 271
BciT130I CCWGG 3 cut(s) 413, 744, 1127
BcoDI GTCTC 1 cut(s) 361
BfaI CTAG 3 cut(s) 41, 575, 1091
BfmI CTRYAG 5 cut(s) 301, 762, 792, 936, 1233
BisI GCNGC 5 cut(s) 246, 301, 936, 1224, 1236
BlsI GCNGC 5 cut(s) 247, 302, 937, 1225, 1237
BmcAI AGTACT 1 cut(s) 238
Bme1390I CCNGG 3 cut(s) 413, 744, 1127
BmeT110I CYCGRG 1 cut(s) 1062
BmgBI CACGTC 1 cut(s) 351
BmiI GGNNCC 2 cut(s) 417, 1157
BmrFI CCNGG 3 cut(s) 413, 744, 1127
BmrI ACTGGG 1 cut(s) 282
BmsI GCATC 3 cut(s) 257, 664, 712
BmuI ACTGGG 1 cut(s) 282
BpiI GAAGAC 1 cut(s) 1080
BplI GAGNNNNNCTC 6 cut(s) 635, 667, 738, 770, 1232, 1264
BpmI CTGGAG 1 cut(s) 726
Bpu10I CCTNAGC 1 cut(s) 15
BsaBI GATNNNNATC 1 cut(s) 324
BsaI GGTCTC 1 cut(s) 361
BsaJI CCNNGG 2 cut(s) 411, 1025
BsaWI WCCGGW 2 cut(s) 370, 418
BsaXI ACNNNNNCTCC 4 cut(s) 150, 180, 933, 963
Bsc4I CCNNNNNNNGG 3 cut(s) 341, 418, 1101
Bse1I ACTGG 2 cut(s) 97, 277
Bse3DI GCAATG 2 cut(s) 616, 1140
Bse8I GATNNNNATC 1 cut(s) 324
BseBI CCWGG 3 cut(s) 413, 744, 1127
BseDI CCNNGG 2 cut(s) 411, 1025
BseGI GGATG 4 cut(s) 248, 283, 442, 679
BseJI GATNNNNATC 1 cut(s) 324
BseLI CCNNNNNNNGG 3 cut(s) 341, 418, 1101
BseMI GCAATG 2 cut(s) 616, 1140
BseMII CTCAG 3 cut(s) 29, 536, 669
BseNI ACTGG 2 cut(s) 97, 277
BseRI GAGGAG 2 cut(s) 632, 702
BseXI GCAGC 5 cut(s) 232, 287, 922, 1210, 1247
BsgI GTGCAG 2 cut(s) 310, 880
Bsh1236I CGCG 3 cut(s) 219, 543, 584
BshFI GGCC 7 cut(s) 96, 205, 269, 566, 647, 750, 1133
BsiHKAI GWGCWC 2 cut(s) 694, 742
BsiHKCI CYCGRG 1 cut(s) 1062
BsiSI CCGG 2 cut(s) 371, 419
BslI CCNNNNNNNGG 3 cut(s) 341, 418, 1101
BsmAI GTCTC 1 cut(s) 361
BsmI GAATGC 1 cut(s) 553
BsnI GGCC 7 cut(s) 96, 205, 269, 566, 647, 750, 1133
Bso31I GGTCTC 1 cut(s) 361
BsoBI CYCGRG 1 cut(s) 1062
Bsp1286I GDGCHC 2 cut(s) 694, 742
Bsp143I GATC 8 cut(s) 51, 214, 230, 325, 782, 1039, 1155, 1216
Bsp19I CCATGG 1 cut(s) 1025
Bsp68I TCGCGA 2 cut(s) 543, 584
BspACI CCGC 3 cut(s) 614, 978, 1153
BspANI GGCC 7 cut(s) 96, 205, 269, 566, 647, 750, 1133
BspCNI CTCAG 3 cut(s) 28, 535, 670
BspFNI CGCG 3 cut(s) 219, 543, 584
BspHI TCATGA 1 cut(s) 142
BspLI GGNNCC 2 cut(s) 417, 1157
BspMAI CTGCAG 3 cut(s) 305, 940, 1237
BspPI GGATC 8 cut(s) 46, 238, 333, 790, 1034, 1150, 1163, 1211
BspQI GCTCTTC 1 cut(s) 564
BspTNI GGTCTC 1 cut(s) 361
BsrDI GCAATG 2 cut(s) 616, 1140
BsrI ACTGG 2 cut(s) 97, 277
BssECI CCNNGG 2 cut(s) 411, 1025
BssMI GATC 8 cut(s) 51, 214, 230, 325, 782, 1039, 1155, 1216
BssSI CACGAG 1 cut(s) 969
BssT1I CCWWGG 1 cut(s) 1025
Bst2BI CACGAG 1 cut(s) 969
Bst2UI CCWGG 3 cut(s) 413, 744, 1127
Bst4CI ACNGT 6 cut(s) 443, 778, 836, 957, 1181, 1291
Bst6I CTCTTC 1 cut(s) 564
BstC8I GCNNGC 4 cut(s) 551, 1096, 1135, 1257
BstDEI CTNAG 4 cut(s) 15, 522, 595, 678
BstDSI CCRYGG 1 cut(s) 1025
BstF5I GGATG 4 cut(s) 248, 283, 442, 679
BstFNI CGCG 3 cut(s) 219, 543, 584
BstKTI GATC 8 cut(s) 54, 217, 233, 328, 785, 1042, 1158, 1219
BstMAI GTCTC 1 cut(s) 361
BstMBI GATC 8 cut(s) 51, 214, 230, 325, 782, 1039, 1155, 1216
BstMWI GCNNNNNNNGC 5 cut(s) 297, 797, 1220, 1232, 1261
BstNI CCWGG 3 cut(s) 413, 744, 1127
BstNSI RCATGY 1 cut(s) 553
BstSCI CCNGG 3 cut(s) 411, 742, 1125
BstSFI CTRYAG 5 cut(s) 301, 762, 792, 936, 1233
BstUI CGCG 3 cut(s) 219, 543, 584
BstV1I GCAGC 5 cut(s) 232, 287, 922, 1210, 1247
BstV2I GAAGAC 1 cut(s) 1080
BstX2I RGATCY 1 cut(s) 1155
BstYI RGATCY 1 cut(s) 1155
BsuRI GGCC 7 cut(s) 96, 205, 269, 566, 647, 750, 1133
BtgI CCRYGG 1 cut(s) 1025
BtgZI GCGATG 2 cut(s) 240, 1004
BtrI CACGTC 1 cut(s) 351
BtsCI GGATG 4 cut(s) 248, 283, 442, 679
BtsI GCAGTG 1 cut(s) 807
BtsIMutI CAGTG 6 cut(s) 270, 448, 807, 962, 1186, 1287
BtuMI TCGCGA 2 cut(s) 543, 584
Cac8I GCNNGC 4 cut(s) 551, 1096, 1135, 1257
CciI TCATGA 1 cut(s) 142
Csp6I GTAC 5 cut(s) 237, 332, 398, 1177, 1243
CviAII CATG 9 cut(s) 56, 143, 457, 512, 550, 1018, 1026, 1189, 1261
CviQI GTAC 5 cut(s) 237, 332, 398, 1177, 1243
DdeI CTNAG 4 cut(s) 15, 522, 595, 678
DpnI GATC 8 cut(s) 53, 216, 232, 327, 784, 1041, 1157, 1218
DpnII GATC 8 cut(s) 51, 214, 230, 325, 782, 1039, 1155, 1216
EaeI YGGCCR 2 cut(s) 267, 564
Eam1104I CTCTTC 1 cut(s) 564
EarI CTCTTC 1 cut(s) 564
EciI GGCGGA 1 cut(s) 1168
Ecl136II GAGCTC 2 cut(s) 692, 740
Eco130I CCWWGG 1 cut(s) 1025
Eco147I AGGCCT 3 cut(s) 647, 750, 1133
Eco24I GRGCYC 2 cut(s) 694, 742
Eco31I GGTCTC 1 cut(s) 361
Eco53kI GAGCTC 2 cut(s) 692, 740
Eco88I CYCGRG 1 cut(s) 1062
EcoICRI GAGCTC 2 cut(s) 692, 740
EcoRII CCWGG 3 cut(s) 411, 742, 1125
EcoT14I CCWWGG 1 cut(s) 1025
EcoT22I ATGCAT 1 cut(s) 555
EcoT38I GRGCYC 2 cut(s) 694, 742
ErhI CCWWGG 1 cut(s) 1025
FaeI CATG 9 cut(s) 59, 146, 460, 515, 553, 1021, 1029, 1192, 1264
FalI AAGNNNNNCTT 2 cut(s) 632, 664
FatI CATG 9 cut(s) 55, 142, 456, 511, 549, 1017, 1025, 1188, 1260
FblI GTMKAC 2 cut(s) 891, 1302
Fnu4HI GCNGC 5 cut(s) 246, 301, 936, 1224, 1236
FokI GGATG 4 cut(s) 235, 290, 449, 686
FriOI GRGCYC 2 cut(s) 694, 742
Fsp4HI GCNGC 5 cut(s) 246, 301, 936, 1224, 1236
FspBI CTAG 3 cut(s) 41, 575, 1091
GluI GCNGC 5 cut(s) 246, 301, 936, 1224, 1236
GsuI CTGGAG 1 cut(s) 726
HaeIII GGCC 7 cut(s) 96, 205, 269, 566, 647, 750, 1133
HapII CCGG 2 cut(s) 371, 419
Hin1II CATG 9 cut(s) 59, 146, 460, 515, 553, 1021, 1029, 1192, 1264
HincII GTYRAC 1 cut(s) 1194
HindII GTYRAC 1 cut(s) 1194
HindIII AAGCTT 1 cut(s) 1253
HinfI GANTC 4 cut(s) 62, 172, 460, 842
HpaII CCGG 2 cut(s) 371, 419
HphI GGTGA 3 cut(s) 111, 1019, 1217
Hpy166II GTNNAC 4 cut(s) 332, 892, 1194, 1303
Hpy188I TCNGA 2 cut(s) 309, 782
Hpy8I GTNNAC 4 cut(s) 332, 892, 1194, 1303
Hpy99I CGWCG 2 cut(s) 782, 1103
HpyAV CCTTC 6 cut(s) 357, 634, 658, 761, 1143, 1169
HpyCH4III ACNGT 6 cut(s) 443, 778, 836, 957, 1181, 1291
HpyCH4IV ACGT 3 cut(s) 209, 350, 387
HpyF10VI GCNNNNNNNGC 5 cut(s) 297, 797, 1220, 1232, 1261
HpyF3I CTNAG 4 cut(s) 15, 522, 595, 678
HpySE526I ACGT 3 cut(s) 209, 350, 387
Hsp92II CATG 9 cut(s) 59, 146, 460, 515, 553, 1021, 1029, 1192, 1264
Kzo9I GATC 8 cut(s) 51, 214, 230, 325, 782, 1039, 1155, 1216
LguI GCTCTTC 1 cut(s) 564
LmnI GCTCC 7 cut(s) 689, 697, 737, 745, 772, 1211, 1272
Lsp1109I GCAGC 5 cut(s) 232, 287, 922, 1210, 1247
LweI GCATC 3 cut(s) 257, 664, 712
MaeI CTAG 3 cut(s) 41, 575, 1091
MaeII ACGT 3 cut(s) 209, 350, 387
MaeIII GTNAC 2 cut(s) 473, 1007
MalI GATC 8 cut(s) 53, 216, 232, 327, 784, 1041, 1157, 1218
MboI GATC 8 cut(s) 51, 214, 230, 325, 782, 1039, 1155, 1216
MboII GAAGA 5 cut(s) 392, 581, 720, 923, 1085
MfeI CAATTG 2 cut(s) 21, 1002
MflI RGATCY 1 cut(s) 1155
MhlI GDGCHC 2 cut(s) 694, 742
MluCI AATT 6 cut(s) 21, 46, 87, 532, 1002, 1086
MlyI GAGTC 1 cut(s) 71
MmeI TCCRAC 4 cut(s) 287, 760, 781, 836
Mph1103I ATGCAT 1 cut(s) 555
MseI TTAA 3 cut(s) 132, 479, 1326
MslI CAYNNNNRTG 1 cut(s) 707
MspI CCGG 2 cut(s) 371, 419
MspR9I CCNGG 3 cut(s) 413, 744, 1127
MunI CAATTG 2 cut(s) 21, 1002
Mva1269I GAATGC 1 cut(s) 553
MvaI CCWGG 3 cut(s) 413, 744, 1127
MvnI CGCG 3 cut(s) 219, 543, 584
MwoI GCNNNNNNNGC 5 cut(s) 297, 797, 1220, 1232, 1261
NcoI CCATGG 1 cut(s) 1025
NdeII GATC 8 cut(s) 51, 214, 230, 325, 782, 1039, 1155, 1216
NlaIII CATG 9 cut(s) 59, 146, 460, 515, 553, 1021, 1029, 1192, 1264
NlaIV GGNNCC 2 cut(s) 417, 1157
NmuCI GTSAC 2 cut(s) 473, 1007
NruI TCGCGA 2 cut(s) 543, 584
NsiI ATGCAT 1 cut(s) 555
NspI RCATGY 1 cut(s) 553
OliI CACNNNNGTG 1 cut(s) 707
PaeI GCATGC 1 cut(s) 553
PaeR7I CTCGAG 1 cut(s) 1062
PagI TCATGA 1 cut(s) 142
PceI AGGCCT 3 cut(s) 647, 750, 1133
PciSI GCTCTTC 1 cut(s) 564
PctI GAATGC 1 cut(s) 553
PfeI GAWTC 3 cut(s) 172, 460, 842
PflFI GACNNNGTC 1 cut(s) 443
PfoI TCCNGGA 1 cut(s) 742
PkrI GCNGC 5 cut(s) 247, 302, 937, 1225, 1237
PleI GAGTC 1 cut(s) 70
PpsI GAGTC 1 cut(s) 70
Psp124BI GAGCTC 2 cut(s) 694, 742
Psp6I CCWGG 3 cut(s) 411, 742, 1125
PspGI CCWGG 3 cut(s) 411, 742, 1125
PspN4I GGNNCC 2 cut(s) 417, 1157
PstI CTGCAG 3 cut(s) 305, 940, 1237
PsuI RGATCY 1 cut(s) 1155
PsyI GACNNNGTC 1 cut(s) 443
RruI TCGCGA 2 cut(s) 543, 584
RsaI GTAC 5 cut(s) 238, 333, 399, 1178, 1244
RsaNI GTAC 5 cut(s) 237, 332, 398, 1177, 1243
RseI CAYNNNNRTG 1 cut(s) 707
SacI GAGCTC 2 cut(s) 694, 742
SapI GCTCTTC 1 cut(s) 564
SaqAI TTAA 3 cut(s) 132, 479, 1326
SatI GCNGC 5 cut(s) 246, 301, 936, 1224, 1236
Sau3AI GATC 8 cut(s) 51, 214, 230, 325, 782, 1039, 1155, 1216
ScaI AGTACT 1 cut(s) 238
SchI GAGTC 1 cut(s) 71
ScrFI CCNGG 3 cut(s) 413, 744, 1127
SduI GDGCHC 2 cut(s) 694, 742
SfaNI GCATC 3 cut(s) 257, 664, 712
SfcI CTRYAG 5 cut(s) 301, 762, 792, 936, 1233
Sfr274I CTCGAG 1 cut(s) 1062
SlaI CTCGAG 1 cut(s) 1062
SmiMI CAYNNNNRTG 1 cut(s) 707
SmlI CTYRAG 1 cut(s) 1062
SmoI CTYRAG 1 cut(s) 1062
SphI GCATGC 1 cut(s) 553
Sse9I AATT 6 cut(s) 21, 46, 87, 532, 1002, 1086
SseBI AGGCCT 3 cut(s) 647, 750, 1133
SsiI CCGC 3 cut(s) 614, 978, 1153
SspMI CTAG 3 cut(s) 41, 575, 1091
SstI GAGCTC 2 cut(s) 694, 742
StuI AGGCCT 3 cut(s) 647, 750, 1133
StyD4I CCNGG 3 cut(s) 411, 742, 1125
StyI CCWWGG 1 cut(s) 1025
TaaI ACNGT 6 cut(s) 443, 778, 836, 957, 1181, 1291
TaiI ACGT 3 cut(s) 212, 353, 390
TaqI TCGA 3 cut(s) 233, 845, 1063
TaqII GACCGA 1 cut(s) 618
TasI AATT 6 cut(s) 21, 46, 87, 532, 1002, 1086
TatI WGTACW 2 cut(s) 236, 1176
TfiI GAWTC 3 cut(s) 172, 460, 842
Tru1I TTAA 3 cut(s) 132, 479, 1326
Tru9I TTAA 3 cut(s) 132, 479, 1326
TscAI CASTG 6 cut(s) 277, 448, 807, 962, 1186, 1294
TseFI GTSAC 2 cut(s) 473, 1007
TseI GCWGC 5 cut(s) 245, 300, 935, 1223, 1235
Tsp45I GTSAC 2 cut(s) 473, 1007
TspDTI ATGAA 5 cut(s) 162, 473, 930, 1034, 1105
TspGWI ACGGA 1 cut(s) 1211
TspRI CASTG 6 cut(s) 277, 448, 807, 962, 1186, 1294
Tth111I GACNNNGTC 1 cut(s) 443
XceI RCATGY 1 cut(s) 553
XhoI CTCGAG 1 cut(s) 1062
XmiI GTMKAC 2 cut(s) 891, 1302
XspI CTAG 3 cut(s) 41, 575, 1091
ZrmI AGTACT 1 cut(s) 238
Zsp2I ATGCAT 1 cut(s) 555
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.