Rroxscaffold_2G00147520

phosphatase 2C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0010053
Physical Location & Seq
Reverse (-)
85315733 .. 85318650
2918 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00147520.1

Sequence Viewer

Length: 1203 bp
ATGGAAGACGTAGCTCAAGCAGTTGCAGTTCCATATAGGCTAGGTAATTTGATTCATGAGGAATCAGCAGTGAAAACCCAAATGGAAATCACTGGTCTCAAGCTAATATCAAATACATCAACCTTGCTATCCAATACTTCTTCAAAACCAAGTCTACCATGTGAGTCCATTTCTTGTGGAAATCAAAGTTACAGAAGCAAAAATCCACAGGACGAAAATAGAGATTTGATTTCTGGTGAAACCATTAATTGGGGAAGTAAGGAGTGCAAGTTCATGCCTTTATGCAATCGGGTTCTAGGTAGTTCATGCTTCAAATCTTTGGTTCATGATGAGGGTGAGATTTGCAATGGATCTAACACTTGTGATGCATCCACCACTTCGGTGAATCGGGCAAAGAAAATTTGTAGGACAAGCTCCAATGGGAAGGTTTTCGATTTGGACCGTGCACCCTTTTGGGGATACACCTCAATCCGTGGAGGGAGACCAGAGATGGAAGATGATGTTGCAGTTATTCCTAGACTTTTGCAGATTCCTATTCAAATGTTAGTGGATGATTCTGACATGAGCCAAAACCCAAGTCAATTAACTGCCCATTTCTTTGGAGTTTATGATGGACATGGGGGTTGTCAGGTTGCTAATTACTGTCGTGAACGAGTGCATTCAGCTTTGGTTGAAGAGATTGAAATTGCTAAAGCAGGCTTGAATGAAAGCACTGGTCAGTCTTTGCAGGAGCAGTGGAAGGAGGCCTTCACCAATTGCTTCATAAAACCAGATAGAGAAGATGAATATGAAAGGATTGAGGCTGCTGGAGGCAAGGTCATACAGTGGGATGGTTCTCGTGTATTTGGTGTTCTTGCCATGTCAAGGTCCATAGGTGACAGATACTTGAAACCATCAATCATACCGGATCCAGAAATCATGTTTGTTTCTCGAGAGAAGGAAGACGAGTGCCTAATCCTAGCCAGTGACGGCTTGTGGGATGTAATAACAAATGAGGAGGCCTGTGACATTGCGCGGAGGCGGATCCTTGTATGGCATAAGAAGTATGGTGATACAGTAGAACGGGGCGAGGGAGCTGATCCTGCAGCCGAAGCTGCAGCAGTGTACCTCTCAAGGCTTGCAATCCAAAAGGGGAGCAAAGACAACATAACTGTTGTAGTGGTAGACCTGAAAGCTTCAAGGAAGTTTAAGAAAAAAATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0001932 GO:0001933 GO:0003674 GO:0003824 GO:0004721 GO:0004722 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005886 GO:0006464 GO:0006469 GO:0006470 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007275 GO:0008150 GO:0008152 GO:0009266 GO:0009314 GO:0009408 GO:0009409 GO:0009414 GO:0009415 GO:0009416 GO:0009628 GO:0009642 GO:0009644 GO:0009719 GO:0009725 GO:0009737 GO:0009787 GO:0009788 GO:0009892 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010035 GO:0010109 GO:0010119 GO:0010205 GO:0010563 GO:0010605 GO:0010646 GO:0010648 GO:0016020 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0019220 GO:0019222 GO:0019538 GO:0019899 GO:0019900 GO:0019901 GO:0022622 GO:0023051 GO:0023057 GO:0031323 GO:0031324 GO:0031399 GO:0031400 GO:0032268 GO:0032269 GO:0032501 GO:0032502 GO:0033673 GO:0033993 GO:0036211 GO:0042221 GO:0042325 GO:0042326 GO:0042548 GO:0042578 GO:0043086 GO:0043155 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043467 GO:0043549 GO:0044092 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045859 GO:0045936 GO:0048364 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0048731 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051174 GO:0051246 GO:0051248 GO:0051338 GO:0051348 GO:0060255 GO:0065007 GO:0065009 GO:0071704 GO:0071944 GO:0080090 GO:0097305 GO:0099402 GO:0140096 GO:1901419 GO:1901420 GO:1901564 GO:1901700 GO:1902456 GO:1905156 GO:1905957 GO:1905958
KEGG Pathways
Metabolic & Signaling
ko04016 ko04075 ko04931 map04016 map04075 map04931
Pfam Domains
Protein Families
PP2C

Protein Analysis

400

Amino Acids

44.36

Weight (kDa)

5.41

Isoelectric Point (pI)

41.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 256 - 383 6.4e-33 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 249
AccI GTMKAC 2 cut(s) 154, 1164
AccII CGCG 1 cut(s) 1015
AciI CCGC 2 cut(s) 1015, 1021
AclWI GGATC 6 cut(s) 358, 902, 915, 1018, 1031, 1073
AcsI RAATTY 2 cut(s) 399, 1197
AfaI GTAC 1 cut(s) 1106
AfiI CCNNNNNNNGG 3 cut(s) 249, 455, 864
AgsI TTSAA 8 cut(s) 144, 313, 539, 674, 683, 703, 889, 1179
AleI CACNNNNGTG 1 cut(s) 380
AloI GAACNNNNNNTCC 2 cut(s) 254, 286
AluBI AGCT 7 cut(s) 14, 103, 414, 665, 1076, 1094, 1175
AluI AGCT 7 cut(s) 14, 103, 414, 665, 1076, 1094, 1175
Alw21I GWGCWC 1 cut(s) 448
Alw26I GTCTC 2 cut(s) 101, 475
Alw44I GTGCAC 1 cut(s) 444
AlwI GGATC 6 cut(s) 358, 902, 915, 1018, 1031, 1073
Ama87I CYCGRG 1 cut(s) 930
AoxI GGCC 2 cut(s) 744, 999
ApaLI GTGCAC 1 cut(s) 444
ApeKI GCWGC 4 cut(s) 803, 1085, 1094, 1097
ApoI RAATTY 2 cut(s) 399, 1197
ArsI GACNNNNNNTTYG 2 cut(s) 562, 594
AseI ATTAAT 1 cut(s) 246
Asp700I GAANNNNTTC 1 cut(s) 428
AspLEI GCGC 1 cut(s) 1015
AspS9I GGNCC 2 cut(s) 439, 867
AsuHPI GGTGA 6 cut(s) 248, 347, 394, 742, 887, 1061
AvaI CYCGRG 1 cut(s) 930
AvaII GGWCC 2 cut(s) 439, 867
BaeGI GKGCMC 1 cut(s) 448
BamHI GGATCC 2 cut(s) 907, 1023
BauI CACGAG 1 cut(s) 837
BbsI GAAGAC 2 cut(s) 12, 948
Bbv12I GWGCWC 1 cut(s) 448
BbvI GCAGC 4 cut(s) 790, 1081, 1097, 1109
BccI CCATC 4 cut(s) 484, 605, 824, 901
BceAI ACGGC 1 cut(s) 985
BciVI GTATCC 1 cut(s) 452
BcoDI GTCTC 2 cut(s) 101, 475
BfaI CTAG 4 cut(s) 41, 296, 516, 959
BfmI CTRYAG 2 cut(s) 1083, 1095
BfuI GTATCC 1 cut(s) 452
BisI GCNGC 4 cut(s) 804, 1086, 1095, 1098
BlsI GCNGC 4 cut(s) 805, 1087, 1096, 1099
Bme18I GGWCC 2 cut(s) 439, 867
BmeT110I CYCGRG 1 cut(s) 930
BmgT120I GGNCC 2 cut(s) 439, 867
BmiI GGNNCC 2 cut(s) 909, 1025
BmsI GCATC 2 cut(s) 355, 377
BpiI GAAGAC 2 cut(s) 12, 948
BpmI CTGGAG 1 cut(s) 828
BpuEI CTTGAG 2 cut(s) 83, 1096
BsaI GGTCTC 2 cut(s) 101, 475
BsaJI CCNNGG 1 cut(s) 472
BsaWI WCCGGW 1 cut(s) 904
BsaXI ACNNNNNCTCC 4 cut(s) 254, 284, 801, 831
Bsc4I CCNNNNNNNGG 3 cut(s) 249, 455, 864
Bse1I ACTGG 3 cut(s) 97, 718, 963
Bse3DI GCAATG 2 cut(s) 352, 1008
BseDI CCNNGG 1 cut(s) 472
BseGI GGATG 4 cut(s) 368, 556, 835, 985
BseLI CCNNNNNNNGG 3 cut(s) 249, 455, 864
BseMI GCAATG 2 cut(s) 352, 1008
BseNI ACTGG 3 cut(s) 97, 718, 963
BseRI GAGGAG 1 cut(s) 1010
BseSI GKGCMC 1 cut(s) 448
BseXI GCAGC 4 cut(s) 790, 1081, 1097, 1109
Bsh1236I CGCG 1 cut(s) 1015
BshFI GGCC 2 cut(s) 746, 1001
BsiHKAI GWGCWC 1 cut(s) 448
BsiHKCI CYCGRG 1 cut(s) 930
BsiSI CCGG 1 cut(s) 905
BslI CCNNNNNNNGG 3 cut(s) 249, 455, 864
BsmAI GTCTC 2 cut(s) 101, 475
BsmI GAATGC 1 cut(s) 658
BsnI GGCC 2 cut(s) 746, 1001
Bso31I GGTCTC 2 cut(s) 101, 475
BsoBI CYCGRG 1 cut(s) 930
Bsp1286I GDGCHC 1 cut(s) 448
Bsp143I GATC 4 cut(s) 350, 907, 1023, 1078
BspACI CCGC 2 cut(s) 1015, 1021
BspANI GGCC 2 cut(s) 746, 1001
BspFNI CGCG 1 cut(s) 1015
BspHI TCATGA 2 cut(s) 55, 325
BspLI GGNNCC 2 cut(s) 909, 1025
BspMAI CTGCAG 2 cut(s) 1087, 1099
BspPI GGATC 6 cut(s) 358, 902, 915, 1018, 1031, 1073
BspTNI GGTCTC 2 cut(s) 101, 475
BsrDI GCAATG 2 cut(s) 352, 1008
BsrI ACTGG 3 cut(s) 97, 718, 963
BssECI CCNNGG 1 cut(s) 472
BssMI GATC 4 cut(s) 350, 907, 1023, 1078
BssSI CACGAG 1 cut(s) 837
Bst2BI CACGAG 1 cut(s) 837
Bst4CI ACNGT 5 cut(s) 443, 644, 825, 1057, 1153
Bst6I CTCTTC 1 cut(s) 669
BstC8I GCNNGC 2 cut(s) 697, 1119
BstDSI CCRYGG 1 cut(s) 472
BstF5I GGATG 4 cut(s) 368, 556, 835, 985
BstFNI CGCG 1 cut(s) 1015
BstHHI GCGC 1 cut(s) 1015
BstKTI GATC 4 cut(s) 353, 910, 1026, 1081
BstMAI GTCTC 2 cut(s) 101, 475
BstMBI GATC 4 cut(s) 350, 907, 1023, 1078
BstMWI GCNNNNNNNGC 3 cut(s) 1082, 1091, 1094
BstSFI CTRYAG 2 cut(s) 1083, 1095
BstSLI GKGCMC 1 cut(s) 448
BstUI CGCG 1 cut(s) 1015
BstV1I GCAGC 4 cut(s) 790, 1081, 1097, 1109
BstV2I GAAGAC 2 cut(s) 12, 948
BstX2I RGATCY 3 cut(s) 350, 907, 1023
BstXI CCANNNNNNTGG 1 cut(s) 599
BstYI RGATCY 3 cut(s) 350, 907, 1023
BsuI GTATCC 1 cut(s) 452
BsuRI GGCC 2 cut(s) 746, 1001
BtgI CCRYGG 1 cut(s) 472
BtsCI GGATG 4 cut(s) 368, 556, 835, 985
BtsI GCAGTG 3 cut(s) 75, 740, 1107
BtsIMutI CAGTG 7 cut(s) 75, 90, 711, 740, 830, 970, 1107
Cac8I GCNNGC 2 cut(s) 697, 1119
CciI TCATGA 2 cut(s) 55, 325
CfoI GCGC 1 cut(s) 1015
Cfr13I GGNCC 2 cut(s) 439, 867
Csp6I GTAC 1 cut(s) 1105
CviAII CATG 9 cut(s) 56, 159, 274, 306, 326, 562, 617, 859, 919
CviQI GTAC 1 cut(s) 1105
DpnI GATC 4 cut(s) 352, 909, 1025, 1080
DpnII GATC 4 cut(s) 350, 907, 1023, 1078
Eam1104I CTCTTC 1 cut(s) 669
EarI CTCTTC 1 cut(s) 669
EciI GGCGGA 1 cut(s) 1036
Eco147I AGGCCT 2 cut(s) 746, 1001
Eco31I GGTCTC 2 cut(s) 101, 475
Eco47I GGWCC 2 cut(s) 439, 867
Eco88I CYCGRG 1 cut(s) 930
EcoT22I ATGCAT 1 cut(s) 370
FaeI CATG 9 cut(s) 59, 162, 277, 309, 329, 565, 620, 862, 922
FalI AAGNNNNNCTT 2 cut(s) 731, 763
FatI CATG 9 cut(s) 55, 158, 273, 305, 325, 561, 616, 858, 918
FblI GTMKAC 2 cut(s) 154, 1164
Fnu4HI GCNGC 4 cut(s) 804, 1086, 1095, 1098
FokI GGATG 4 cut(s) 355, 563, 842, 992
Fsp4HI GCNGC 4 cut(s) 804, 1086, 1095, 1098
FspBI CTAG 4 cut(s) 41, 296, 516, 959
GlaI GCGC 1 cut(s) 1014
GluI GCNGC 4 cut(s) 804, 1086, 1095, 1098
GsuI CTGGAG 1 cut(s) 828
HaeIII GGCC 2 cut(s) 746, 1001
HapII CCGG 1 cut(s) 905
HhaI GCGC 1 cut(s) 1015
Hin1II CATG 9 cut(s) 59, 162, 277, 309, 329, 565, 620, 862, 922
Hin6I GCGC 1 cut(s) 1013
HinP1I GCGC 1 cut(s) 1013
HindIII AAGCTT 1 cut(s) 1173
HinfI GANTC 6 cut(s) 52, 62, 164, 385, 529, 554
HpaII CCGG 1 cut(s) 905
HphI GGTGA 6 cut(s) 248, 347, 394, 742, 887, 1061
Hpy166II GTNNAC 5 cut(s) 155, 446, 650, 1105, 1165
Hpy188I TCNGA 1 cut(s) 559
Hpy188III TCNNGA 6 cut(s) 56, 326, 647, 911, 930, 932
Hpy8I GTNNAC 5 cut(s) 155, 446, 650, 1105, 1165
HpyAV CCTTC 4 cut(s) 418, 733, 757, 931
HpyCH4III ACNGT 5 cut(s) 443, 644, 825, 1057, 1153
HpyCH4IV ACGT 1 cut(s) 9
HpyF10VI GCNNNNNNNGC 3 cut(s) 1082, 1091, 1094
HpySE526I ACGT 1 cut(s) 9
Hsp92II CATG 9 cut(s) 59, 162, 277, 309, 329, 565, 620, 862, 922
HspAI GCGC 1 cut(s) 1013
Kzo9I GATC 4 cut(s) 350, 907, 1023, 1078
LmnI GCTCC 4 cut(s) 419, 730, 1073, 1134
Lsp1109I GCAGC 4 cut(s) 790, 1081, 1097, 1109
LweI GCATC 2 cut(s) 355, 377
MaeI CTAG 4 cut(s) 41, 296, 516, 959
MaeII ACGT 1 cut(s) 9
MaeIII GTNAC 4 cut(s) 188, 875, 965, 1004
MalI GATC 4 cut(s) 352, 909, 1025, 1080
MboI GATC 4 cut(s) 350, 907, 1023, 1078
MboII GAAGA 6 cut(s) 17, 132, 506, 686, 791, 953
MfeI CAATTG 1 cut(s) 754
MflI RGATCY 3 cut(s) 350, 907, 1023
MhlI GDGCHC 1 cut(s) 448
MluCI AATT 8 cut(s) 46, 247, 399, 581, 637, 684, 754, 1197
MlyI GAGTC 1 cut(s) 173
Mph1103I ATGCAT 1 cut(s) 370
MroXI GAANNNNTTC 1 cut(s) 428
MseI TTAA 4 cut(s) 246, 584, 1188, 1201
MslI CAYNNNNRTG 1 cut(s) 380
MspI CCGG 1 cut(s) 905
MunI CAATTG 1 cut(s) 754
Mva1269I GAATGC 1 cut(s) 658
MvnI CGCG 1 cut(s) 1015
MwoI GCNNNNNNNGC 3 cut(s) 1082, 1091, 1094
NdeII GATC 4 cut(s) 350, 907, 1023, 1078
NlaIII CATG 9 cut(s) 59, 162, 277, 309, 329, 565, 620, 862, 922
NlaIV GGNNCC 2 cut(s) 909, 1025
NmuCI GTSAC 3 cut(s) 875, 965, 1004
NsiI ATGCAT 1 cut(s) 370
OliI CACNNNNGTG 1 cut(s) 380
PaeR7I CTCGAG 1 cut(s) 930
PagI TCATGA 2 cut(s) 55, 325
PceI AGGCCT 2 cut(s) 746, 1001
PctI GAATGC 1 cut(s) 658
PdmI GAANNNNTTC 1 cut(s) 428
PfeI GAWTC 5 cut(s) 52, 62, 385, 529, 554
PflMI CCANNNNNTGG 1 cut(s) 249
PkrI GCNGC 4 cut(s) 805, 1087, 1096, 1099
PleI GAGTC 1 cut(s) 172
PpsI GAGTC 1 cut(s) 172
PshBI ATTAAT 1 cut(s) 246
PspN4I GGNNCC 2 cut(s) 909, 1025
PspPI GGNCC 2 cut(s) 439, 867
PsrI GAACNNNNNNTAC 2 cut(s) 834, 866
PstI CTGCAG 2 cut(s) 1087, 1099
PsuI RGATCY 3 cut(s) 350, 907, 1023
RsaI GTAC 1 cut(s) 1106
RsaNI GTAC 1 cut(s) 1105
RseI CAYNNNNRTG 1 cut(s) 380
SaqAI TTAA 4 cut(s) 246, 584, 1188, 1201
SatI GCNGC 4 cut(s) 804, 1086, 1095, 1098
Sau3AI GATC 4 cut(s) 350, 907, 1023, 1078
Sau96I GGNCC 2 cut(s) 439, 867
SchI GAGTC 1 cut(s) 173
SduI GDGCHC 1 cut(s) 448
SfaNI GCATC 2 cut(s) 355, 377
SfcI CTRYAG 2 cut(s) 1083, 1095
Sfr274I CTCGAG 1 cut(s) 930
SinI GGWCC 2 cut(s) 439, 867
SlaI CTCGAG 1 cut(s) 930
SmiMI CAYNNNNRTG 1 cut(s) 380
SmlI CTYRAG 4 cut(s) 15, 98, 930, 1111
SmoI CTYRAG 4 cut(s) 15, 98, 930, 1111
Sse9I AATT 8 cut(s) 46, 247, 399, 581, 637, 684, 754, 1197
SseBI AGGCCT 2 cut(s) 746, 1001
SsiI CCGC 2 cut(s) 1015, 1021
SspMI CTAG 4 cut(s) 41, 296, 516, 959
StuI AGGCCT 2 cut(s) 746, 1001
TaaI ACNGT 5 cut(s) 443, 644, 825, 1057, 1153
TaiI ACGT 1 cut(s) 12
TaqI TCGA 2 cut(s) 432, 931
TasI AATT 8 cut(s) 46, 247, 399, 581, 637, 684, 754, 1197
TfiI GAWTC 5 cut(s) 52, 62, 385, 529, 554
Tru1I TTAA 4 cut(s) 246, 584, 1188, 1201
Tru9I TTAA 4 cut(s) 246, 584, 1188, 1201
TscAI CASTG 7 cut(s) 75, 97, 718, 740, 830, 970, 1107
TseFI GTSAC 3 cut(s) 875, 965, 1004
TseI GCWGC 4 cut(s) 803, 1085, 1094, 1097
Tsp45I GTSAC 3 cut(s) 875, 965, 1004
TspDTI ATGAA 8 cut(s) 44, 262, 294, 314, 720, 751, 798, 804
TspGWI ACGGA 1 cut(s) 461
TspRI CASTG 7 cut(s) 75, 97, 718, 740, 830, 970, 1107
Van91I CCANNNNNTGG 1 cut(s) 249
VneI GTGCAC 1 cut(s) 444
VpaK11BI GGWCC 2 cut(s) 439, 867
VspI ATTAAT 1 cut(s) 246
XapI RAATTY 2 cut(s) 399, 1197
XhoI CTCGAG 1 cut(s) 930
XmiI GTMKAC 2 cut(s) 154, 1164
XmnI GAANNNNTTC 1 cut(s) 428
XspI CTAG 4 cut(s) 41, 296, 516, 959
Zsp2I ATGCAT 1 cut(s) 370
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.