Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000002

Orthogroup ID

OG0002518

Physical Location & Seq

Genomic Coordinates Forward (+)

3199047 .. 3205463

Gene Structure 6417 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug02G0040700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2187 bp

ATGCTCCGTCTTTGGCGTCTTAGAAGAGTTAGTGCTCTATTTTCCAGATTGGAGAAAGATAGGAACTATAATTACTTTTGGGTTCGTTGTGCAAAACTTATTTGTGTCACCCTGTTTGCGGTTCACTGTGCTGGATGCTTCTATTACCTTCTTGCAGCCCGTTACCGTGACCCCAAGAACACATGGATTGGAAAAACAATGGAAGATTTTCTTCATCAAAGTCTGTGGATTCGATATGTGACTACAGTTTATTGGTCTATCACTACACTCACAACCGTTGGCTACGGAGATTTGCATCCTGTCAATACAAGGGAGATGATCTTTGACATATTCTACATGCTTTTCAACCTTGGCTTGACATCATACTTGATTGGAAATATGACCAACTTGGTTGTCCATGGGACCAGTCGAACACGAAAATTTAGGGATACCATACAAGCTGCCTCCAGTTTTGCACAAAGGAACCAACTGCCTGTTCGCCTGCAGGATCAGATGCTTGCCCACTTGTGTCTGAAGTTCAGGACAGATGCAGAGGGACTCCAGCAACAAGAGACTCTTGATTCACTTCCGAAAGCCATACGCTCAAGTATTTCACATTATCTGTTTTACTCTCTTGTCGATAAGGTGTACTTGTTTCATGGTGTTTCAAATGACTTGCTTTTTCAGTTGGTCTCAGAGATGAAAGCTGAGTATTTTCCTCCCAAAGAAGATGTAATATTGCAGAATGAAGCACCTACAGATTTCTACATACTTGTCACTGGTGCTGCGGATTTAGTGGTCCTCAAGAATGGAGTTGAACAGGTCATTGGTGAGGCAAAAACTGGTGATCTTATTGGTGAAATTGGGGTACTCTGTTATAGGCCACAGCTTTTTACCGTTAGGACCAAAAGATTGAGTCAGCTACTCCGGCTGAACCGAACTACATTCTTAAGCATAGTTCAAGCTAATGTTGGGGATGGAACTATAATAATGAATAACCTCCTCCAGCATTTGAAAGAGGTCAAGGATCCATATATGGAGGGAGTTTTGCTGGAGACTGAGAACATGCTAGCACGTGGTAGACTGGATCTACCTCTCAGTCTATGCTTTGCAGCAATTAGAGGAGATGACTTGCTGTTGCATCAGTTGTTGAGACGGGGTTTGGATCCAAATGAATCAGACAATAATGGCAGAACAGCTCTGCATATAGCTGCATCCCAAGGAAGTGAGAACTGTGTGCTTCTACTATTGGACTATGGAGCACTTCCTAACAGTAGAGACTCGGAGGGAAATGTACCACTTTGGGAGGCAATACAGAACGATCATGAATCGATTGCCAAACTGCTGGTGGACAACGGTGCAACTTTGAATGCTGGGGATGTTGGTCACTTTGCTTGCACTGCTGCTGAGCAAAACAGGTTGGACTTGCTCAATGAAATAGTTCAACATGGAGGAGATGTCACAGCTCCTAGAAGCAATGGAACCACAGCTCTCCATGTTGCAGTGTCCGAAGACAACATTGAAATTGTGAAATTCCTTTTGGACAAAGGGGCAGATATTGACAAACCAGACCTCCATGGCTGGACCGCGAGAGCTCTAGCCGAGCAACAAGGACATGAAGAGATAAAGAACCTTTTTGAATCTAGTAAACAGCCTAAAGTTCAATCTGTTATCACCATTCCTACTGAGCGAAAGACTGGAATTCGCTTCCTTGGGAGATTTACAAGCGAGCCAAATATCCATCCTCCCTCCCAGGGAGGCGCATTTCTAGTAGCAGATGGGGGATCATGGGGCCGAAGCCGCCCAAGGCGTAGGACTAACAACTTCCACAACTCACTTTTTGGGATGATGTCAGCTGCACACACAGGGGAGAAGGATTTGTTCTTCTCCTCAGTTAGGGAGACCGTCGAAACCACTCCTAAGGGTATTGGGAGTAATCCTAACCCTGCTAGAGTGACAATTAGTTTCCCTGAAAAGGGTGAGGTTTCAGGGAAACTTGTATTACTTCCTGCCAGTTTTGAGGAGTTACTCGAGGTTGGTGCTAAGAAATTCGGTTTCACTCCGGCTAAAGTTCTGAGCAAAGATGGAGGAGCTGAAATTGACGATATAGAGGTGATTAGAGATGGTGATCATCTTATATTTGTCAGTGGCCAAGATGAACGTCAAGAATGTAATAGCGAAGATGCTCCGACTAAAAAAGATGTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2

Protein Analysis

728

Amino Acids

81.44

Weight (kDa)

6.09

Isoelectric Point (pI)

38.1

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ion_trans	PF00520	1 - 138	3.1e-26	Ion transport protein
Ion_trans_2	PF07885	41 - 131	1.2e-12	Ion channel
cNMP_binding	PF00027	231 - 316	1.9e-14	Cyclic nucleotide-binding domain
Ank_2	PF12796	361 - 452	4.6e-18	Ankyrin repeats (3 copies)
Ank_4	PF13637	361 - 410	5.7e-09	Ankyrin repeats (many copies)
Ank_5	PF13857	376 - 427	2.1e-08	Ankyrin repeats (many copies)
Ank	PF00023	389 - 420	3.2e-07	Ankyrin repeat
Ank_2	PF12796	460 - 539	3.8e-12	Ankyrin repeats (3 copies)
Ank_4	PF13637	460 - 507	9e-08	Ankyrin repeats (many copies)
Ank_5	PF13857	474 - 527	7.5e-08	Ankyrin repeats (many copies)
Ank	PF00023	486 - 517	3e-08	Ankyrin repeat
KHA	PF11834	644 - 707	1.8e-23	KHA, dimerisation domain of potassium ion channel

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0002518)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G09190
fragaria_vesca	FvH4_1g08040
malus_domestica	MD02G1085000.v1.1
prunus_persica	Prupe.7G204600_v2.0.a1
pyrus_communis	pycom02g06780 pycom02g20400
rosa_chinensis	RchiOBHm_Chr1g0349371 RchiOBHm_Chr1g0365421 RchiOBHm_Chr2g0094071 RchiOBHm_Chr2g0100501 RchiOBHm_Chr2g0145241 RchiOBHm_Chr5g0027681
rosa_laevigata	RLG00000016453 RLG00000016978 RLG00000028565
rosa_multiflora	Rmu_sc0000528.1_g000021 Rmu_sc0001942.1_g000078 Rmu_sc0001989.1_g000005 Rmu_sc0001989.1_g000006 Rmu_sc0002834.1_g000001 Rmu_sc0010460.1_g000009 Rmu_sc0015757.1_g000001 Rmu_sc0023774.1_g000001 Rmu_ssc0000469.1_g000004
rosa_roxburghii	Rroxscaffold_1G00065660 Rroxscaffold_2G00147500
rosa_rugosa	Rorug01G0191200 Rorug01G0245800 Rorug01G0404500 Rorug02G0040700 Rorug02G0042900 Rorug02G0088700 Rorug02G0306900 Rorug02G0425200 RorugPtG0000800
rosa_samantha	Rh1BG121500 Rh2BG141500 Rh2BG455200 Rh2CG090800 Rh2CG143700 Rh2DG465200

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	1 cut(s)	1060
AccII	CGCG	1 cut(s)	1568
AciI	CCGC	4 cut(s)	119, 767, 1566, 1780
AclWI	GGATC	7 cut(s)	495, 1001, 1014, 1074, 1139, 1152, 1771
AcoI	YGGCCR	1 cut(s)	2128
AcsI	RAATTY	4 cut(s)	419, 1511, 1680, 2027
AcuI	CTGAAG	1 cut(s)	533
AcvI	CACGTG	1 cut(s)	1055
AcyI	GRCGYC	1 cut(s)	16
AfaI	GTAC	3 cut(s)	629, 849, 1275
AfiI	CCNNNNNNNGG	5 cut(s)	118, 1733, 1875, 1954, 1955
AflII	CTTAAG	1 cut(s)	928
AjnI	CCWGG	1 cut(s)	1731
AjuI	GAANNNNNNNTTGG	6 cut(s)	459, 491, 789, 821, 1502, 1534
Alw21I	GWGCWC	3 cut(s)	37, 1243, 1576
Alw26I	GTCTC	6 cut(s)	545, 676, 1028, 1126, 1251, 1874
AlwI	GGATC	7 cut(s)	495, 1001, 1014, 1074, 1139, 1152, 1771
Ama87I	CYCGRG	1 cut(s)	2009
AoxI	GGCC	3 cut(s)	860, 1771, 2128
ApeKI	GCWGC	7 cut(s)	155, 440, 764, 1091, 1190, 1382, 1835
ApoI	RAATTY	4 cut(s)	419, 1511, 1680, 2027
Asp700I	GAANNNNTTC	2 cut(s)	207, 1419
AspLEI	GCGC	1 cut(s)	1742
AspS9I	GGNCC	5 cut(s)	402, 778, 882, 1563, 1771
AsuHPI	GGTGA	8 cut(s)	100, 821, 836, 848, 1645, 1970, 2104, 2117
AsuNHI	GCTAGC	1 cut(s)	1048
AvaI	CYCGRG	1 cut(s)	2009
AvaII	GGWCC	4 cut(s)	402, 778, 882, 1563
AxyI	CCTNAGG	1 cut(s)	1899
BalI	TGGCCA	1 cut(s)	2130
BamHI	GGATCC	2 cut(s)	1006, 1144
BanII	GRGCYC	1 cut(s)	1576
BbrPI	CACGTG	1 cut(s)	1055
BbsI	GAAGAC	1 cut(s)	1497
Bbv12I	GWGCWC	3 cut(s)	37, 1243, 1576
BbvI	GCAGC	7 cut(s)	167, 427, 751, 1103, 1177, 1369, 1822
BccI	CCATC	5 cut(s)	950, 1728, 1751, 2057, 2096
BcgI	CGANNNNNNTGC	2 cut(s)	2000, 2034
BciT130I	CCWGG	1 cut(s)	1733
BciVI	GTATCC	1 cut(s)	421
BclI	TGATCA	1 cut(s)	2107
BcoDI	GTCTC	6 cut(s)	545, 676, 1028, 1126, 1251, 1874
BfaI	CTAG	6 cut(s)	1049, 1449, 1577, 1623, 1748, 1929
BfmI	CTRYAG	3 cut(s)	243, 482, 735
BfrI	CTTAAG	1 cut(s)	928
BfuI	GTATCC	1 cut(s)	421
BisI	GCNGC	8 cut(s)	156, 441, 765, 1092, 1191, 1383, 1780, 1836
BlpI	GCTNAGC	1 cut(s)	1386
BlsI	GCNGC	8 cut(s)	157, 442, 766, 1093, 1192, 1384, 1781, 1837
Bme1390I	CCNGG	1 cut(s)	1733
Bme18I	GGWCC	4 cut(s)	402, 778, 882, 1563
BmeT110I	CYCGRG	1 cut(s)	2009
BmgT120I	GGNCC	5 cut(s)	402, 778, 882, 1563, 1771
BmiI	GGNNCC	6 cut(s)	403, 464, 1008, 1146, 1462, 1772
BmrFI	CCNGG	1 cut(s)	1733
BmsI	GCATC	7 cut(s)	125, 304, 483, 517, 1129, 1202, 2152
BmtI	GCTAGC	1 cut(s)	1052
BpiI	GAAGAC	1 cut(s)	1497
BpmI	CTGGAG	4 cut(s)	430, 524, 968, 1052
Bpu1102I	GCTNAGC	1 cut(s)	1386
BpuEI	CTTGAG	2 cut(s)	568, 767
Bsa29I	ATCGAT	1 cut(s)	1310
BsaAI	YACGTR	1 cut(s)	1055
BsaBI	GATNNNNATC	2 cut(s)	294, 2106
BsaHI	GRCGYC	1 cut(s)	16
BsaI	GGTCTC	2 cut(s)	676, 1874
BsaJI	CCNNGG	8 cut(s)	349, 397, 1198, 1555, 1690, 1731, 1732, 1784
BsaXI	ACNNNNNCTCC	4 cut(s)	1422, 1452, 1536, 1566
Bsc4I	CCNNNNNNNGG	5 cut(s)	118, 1733, 1875, 1954, 1955
Bse1I	ACTGG	7 cut(s)	405, 447, 763, 826, 1068, 1681, 1992
Bse21I	CCTNAGG	1 cut(s)	1899
Bse3DI	GCAATG	1 cut(s)	1462
Bse8I	GATNNNNATC	2 cut(s)	294, 2106
BseBI	CCWGG	1 cut(s)	1733
BseCI	ATCGAT	1 cut(s)	1310
BseDI	CCNNGG	8 cut(s)	349, 397, 1198, 1555, 1690, 1731, 1732, 1784
BseGI	GGATG	7 cut(s)	140, 295, 961, 1193, 1363, 1720, 1830
BseJI	GATNNNNATC	2 cut(s)	294, 2106
BseLI	CCNNNNNNNGG	5 cut(s)	118, 1733, 1875, 1954, 1955
BseMI	GCAATG	1 cut(s)	1462
BseMII	CTCAG	8 cut(s)	678, 687, 1029, 1090, 1377, 1656, 1884, 2045
BseNI	ACTGG	7 cut(s)	405, 447, 763, 826, 1068, 1681, 1992
BseRI	GAGGAG	6 cut(s)	971, 1116, 1446, 1858, 2015, 2082
BseXI	GCAGC	7 cut(s)	167, 427, 751, 1103, 1177, 1369, 1822
BseYI	CCCAGC	1 cut(s)	1352
BsgI	GTGCAG	1 cut(s)	1821
Bsh1236I	CGCG	1 cut(s)	1568
BshFI	GGCC	3 cut(s)	862, 1773, 2130
BshVI	ATCGAT	1 cut(s)	1310
BsiHKAI	GWGCWC	3 cut(s)	37, 1243, 1576
BsiHKCI	CYCGRG	1 cut(s)	2009
BsiSI	CCGG	2 cut(s)	907, 2042
BslFI	GGGAC	2 cut(s)	415, 549
BslI	CCNNNNNNNGG	5 cut(s)	118, 1733, 1875, 1954, 1955
BsmAI	GTCTC	6 cut(s)	545, 676, 1028, 1126, 1251, 1874
BsmBI	CGTCTC	1 cut(s)	1126
BsmFI	GGGAC	2 cut(s)	415, 549
BsmI	GAATGC	1 cut(s)	1354
BsnI	GGCC	3 cut(s)	862, 1773, 2130
Bso31I	GGTCTC	2 cut(s)	676, 1874
BsoBI	CYCGRG	1 cut(s)	2009
Bsp1286I	GDGCHC	3 cut(s)	37, 1243, 1576
Bsp143I	GATC	9 cut(s)	318, 487, 826, 1006, 1066, 1144, 1300, 1763, 2107
Bsp1720I	GCTNAGC	1 cut(s)	1386
Bsp19I	CCATGG	2 cut(s)	397, 1555
BspACI	CCGC	4 cut(s)	119, 767, 1566, 1780
BspANI	GGCC	3 cut(s)	862, 1773, 2130
BspCNI	CTCAG	8 cut(s)	679, 686, 1030, 1089, 1378, 1657, 1883, 2046
BspDI	ATCGAT	1 cut(s)	1310
BspFNI	CGCG	1 cut(s)	1568
BspHI	TCATGA	1 cut(s)	1303
BspLI	GGNNCC	6 cut(s)	403, 464, 1008, 1146, 1462, 1772
BspMAI	CTGCAG	1 cut(s)	486
BspOI	GCTAGC	1 cut(s)	1052
BspPI	GGATC	7 cut(s)	495, 1001, 1014, 1074, 1139, 1152, 1771
BspTI	CTTAAG	1 cut(s)	928
BspTNI	GGTCTC	2 cut(s)	676, 1874
BsrDI	GCAATG	1 cut(s)	1462
BsrI	ACTGG	7 cut(s)	405, 447, 763, 826, 1068, 1681, 1992
BssECI	CCNNGG	8 cut(s)	349, 397, 1198, 1555, 1690, 1731, 1732, 1784
BssMI	GATC	9 cut(s)	318, 487, 826, 1006, 1066, 1144, 1300, 1763, 2107
BssNI	GRCGYC	1 cut(s)	16
BssT1I	CCWWGG	6 cut(s)	349, 397, 1198, 1555, 1690, 1784
Bst2UI	CCWGG	1 cut(s)	1733
Bst4CI	ACNGT	9 cut(s)	128, 167, 247, 277, 877, 1214, 1253, 1337, 1885
Bst6I	CTCTTC	2 cut(s)	19, 1593
BstACI	GRCGYC	1 cut(s)	16
BstAFI	CTTAAG	1 cut(s)	928
BstBAI	YACGTR	1 cut(s)	1055
BstC8I	GCNNGC	5 cut(s)	482, 498, 1050, 1375, 1709
BstDSI	CCRYGG	2 cut(s)	397, 1555
BstENI	CCTNNNNNAGG	1 cut(s)	1873
BstF5I	GGATG	7 cut(s)	140, 295, 961, 1193, 1363, 1720, 1830
BstFNI	CGCG	1 cut(s)	1568
BstHHI	GCGC	1 cut(s)	1742
BstKTI	GATC	9 cut(s)	321, 490, 829, 1009, 1069, 1147, 1303, 1766, 2110
BstMAI	GTCTC	6 cut(s)	545, 676, 1028, 1126, 1251, 1874
BstMBI	GATC	9 cut(s)	318, 487, 826, 1006, 1066, 1144, 1300, 1763, 2107
BstMWI	GCNNNNNNNGC	3 cut(s)	907, 1379, 1779
BstNI	CCWGG	1 cut(s)	1733
BstNSI	RCATGY	2 cut(s)	340, 1048
BstSCI	CCNGG	1 cut(s)	1731
BstSFI	CTRYAG	3 cut(s)	243, 482, 735
BstUI	CGCG	1 cut(s)	1568
BstV1I	GCAGC	7 cut(s)	167, 427, 751, 1103, 1177, 1369, 1822
BstV2I	GAAGAC	1 cut(s)	1497
BstX2I	RGATCY	3 cut(s)	1006, 1066, 1144
BstXI	CCANNNNNNTGG	1 cut(s)	1324
BstYI	RGATCY	3 cut(s)	1006, 1066, 1144
Bsu15I	ATCGAT	1 cut(s)	1310
Bsu36I	CCTNAGG	1 cut(s)	1899
BsuI	GTATCC	1 cut(s)	421
BsuRI	GGCC	3 cut(s)	862, 1773, 2130
BsuTUI	ATCGAT	1 cut(s)	1310
BtgI	CCRYGG	2 cut(s)	397, 1555
BtsCI	GGATG	7 cut(s)	140, 295, 961, 1193, 1363, 1720, 1830
BtsI	GCAGTG	2 cut(s)	1377, 1488
BtsIMutI	CAGTG	5 cut(s)	124, 756, 1377, 1488, 2131
Cac8I	GCNNGC	5 cut(s)	482, 498, 1050, 1375, 1709
CciI	TCATGA	1 cut(s)	1303
CfoI	GCGC	1 cut(s)	1742
Cfr13I	GGNCC	5 cut(s)	402, 778, 882, 1563, 1771
ClaI	ATCGAT	1 cut(s)	1310
CseI	GACGC	1 cut(s)	5
Csp6I	GTAC	3 cut(s)	628, 848, 1274
CspCI	CAANNNNNGTGG	2 cut(s)	206, 241
CviQI	GTAC	3 cut(s)	628, 848, 1274
DpnI	GATC	9 cut(s)	320, 489, 828, 1008, 1068, 1146, 1302, 1765, 2109
DpnII	GATC	9 cut(s)	318, 487, 826, 1006, 1066, 1144, 1300, 1763, 2107
EaeI	YGGCCR	1 cut(s)	2128
Eam1104I	CTCTTC	2 cut(s)	19, 1593
EarI	CTCTTC	2 cut(s)	19, 1593
Ecl136II	GAGCTC	1 cut(s)	1574
Eco130I	CCWWGG	6 cut(s)	349, 397, 1198, 1555, 1690, 1784
Eco24I	GRGCYC	1 cut(s)	1576
Eco31I	GGTCTC	2 cut(s)	676, 1874
Eco47I	GGWCC	4 cut(s)	402, 778, 882, 1563
Eco53kI	GAGCTC	1 cut(s)	1574
Eco57I	CTGAAG	1 cut(s)	533
Eco72I	CACGTG	1 cut(s)	1055
Eco81I	CCTNAGG	1 cut(s)	1899
Eco88I	CYCGRG	1 cut(s)	2009
EcoICRI	GAGCTC	1 cut(s)	1574
EcoNI	CCTNNNNNAGG	1 cut(s)	1873
EcoRI	GAATTC	1 cut(s)	1680
EcoRII	CCWGG	1 cut(s)	1731
EcoT14I	CCWWGG	6 cut(s)	349, 397, 1198, 1555, 1690, 1784
EcoT38I	GRGCYC	1 cut(s)	1576
ErhI	CCWWGG	6 cut(s)	349, 397, 1198, 1555, 1690, 1784
Esp3I	CGTCTC	1 cut(s)	1126
FalI	AAGNNNNNCTT	6 cut(s)	195, 227, 540, 572, 614, 646
FaqI	GGGAC	2 cut(s)	415, 549
FbaI	TGATCA	1 cut(s)	2107
FblI	GTMKAC	1 cut(s)	1060
Fnu4HI	GCNGC	8 cut(s)	156, 441, 765, 1092, 1191, 1383, 1780, 1836
FokI	GGATG	7 cut(s)	147, 282, 968, 1180, 1370, 1707, 1837
FriOI	GRGCYC	1 cut(s)	1576
Fsp4HI	GCNGC	8 cut(s)	156, 441, 765, 1092, 1191, 1383, 1780, 1836
FspBI	CTAG	6 cut(s)	1049, 1449, 1577, 1623, 1748, 1929
GlaI	GCGC	1 cut(s)	1741
GluI	GCNGC	8 cut(s)	156, 441, 765, 1092, 1191, 1383, 1780, 1836
GsaI	CCCAGC	1 cut(s)	1356
GsuI	CTGGAG	4 cut(s)	430, 524, 968, 1052
HaeIII	GGCC	3 cut(s)	862, 1773, 2130
HapII	CCGG	2 cut(s)	907, 2042
HgaI	GACGC	1 cut(s)	5
HhaI	GCGC	1 cut(s)	1742
Hin1I	GRCGYC	1 cut(s)	16
Hin6I	GCGC	1 cut(s)	1740
HinP1I	GCGC	1 cut(s)	1740
HinfI	GANTC	9 cut(s)	229, 537, 553, 560, 895, 1154, 1259, 1307, 1619
HpaII	CCGG	2 cut(s)	907, 2042
HphI	GGTGA	8 cut(s)	100, 821, 836, 848, 1645, 1970, 2104, 2117
Hpy166II	GTNNAC	5 cut(s)	124, 628, 1061, 1330, 1628
Hpy188I	TCNGA	9 cut(s)	492, 513, 570, 676, 1159, 1264, 1489, 2055, 2169
Hpy188III	TCNNGA	6 cut(s)	45, 520, 557, 784, 1304, 2144
Hpy8I	GTNNAC	5 cut(s)	124, 628, 1061, 1330, 1628
Hpy99I	CGWCG	1 cut(s)	1889
HpyAV	CCTTC	2 cut(s)	158, 1846
HpyCH4III	ACNGT	9 cut(s)	128, 167, 247, 277, 877, 1214, 1253, 1337, 1885
HpyCH4IV	ACGT	2 cut(s)	1054, 2140
HpyF10VI	GCNNNNNNNGC	3 cut(s)	907, 1379, 1779
HpySE526I	ACGT	2 cut(s)	1054, 2140
Hsp92I	GRCGYC	1 cut(s)	16
HspAI	GCGC	1 cut(s)	1740
Ksp22I	TGATCA	1 cut(s)	2107
Kzo9I	GATC	9 cut(s)	318, 487, 826, 1006, 1066, 1144, 1300, 1763, 2107
LmnI	GCTCC	5 cut(s)	9, 1238, 1450, 2069, 2170
Lsp1109I	GCAGC	7 cut(s)	167, 427, 751, 1103, 1177, 1369, 1822
LweI	GCATC	7 cut(s)	125, 304, 483, 517, 1129, 1202, 2152
MaeI	CTAG	6 cut(s)	1049, 1449, 1577, 1623, 1748, 1929
MaeII	ACGT	2 cut(s)	1054, 2140
MaeIII	GTNAC	9 cut(s)	106, 161, 167, 238, 754, 1364, 1438, 1933, 2004
MalI	GATC	9 cut(s)	320, 489, 828, 1008, 1068, 1146, 1302, 1765, 2109
MboI	GATC	9 cut(s)	318, 487, 826, 1006, 1066, 1144, 1300, 1763, 2107
MboII	GAAGA	8 cut(s)	36, 203, 215, 719, 1502, 1610, 1855, 2171
MflI	RGATCY	3 cut(s)	1006, 1066, 1144
MhlI	GDGCHC	3 cut(s)	37, 1243, 1576
MlsI	TGGCCA	1 cut(s)	2130
MluNI	TGGCCA	1 cut(s)	2130
MlyI	GAGTC	4 cut(s)	531, 547, 904, 1253
MmeI	TCCRAC	1 cut(s)	1380
Mox20I	TGGCCA	1 cut(s)	2130
MroXI	GAANNNNTTC	2 cut(s)	207, 1419
MscI	TGGCCA	1 cut(s)	2130
MseI	TTAA	1 cut(s)	929
Msp20I	TGGCCA	1 cut(s)	2130
MspA1I	CMGCKG	1 cut(s)	1835
MspCI	CTTAAG	1 cut(s)	928
MspI	CCGG	2 cut(s)	907, 2042
MspR9I	CCNGG	1 cut(s)	1733
Mva1269I	GAATGC	1 cut(s)	1354
MvaI	CCWGG	1 cut(s)	1733
MvnI	CGCG	1 cut(s)	1568
MwoI	GCNNNNNNNGC	3 cut(s)	907, 1379, 1779
NcoI	CCATGG	2 cut(s)	397, 1555
NdeII	GATC	9 cut(s)	318, 487, 826, 1006, 1066, 1144, 1300, 1763, 2107
NheI	GCTAGC	1 cut(s)	1048
NlaIV	GGNNCC	6 cut(s)	403, 464, 1008, 1146, 1462, 1772
NmeAIII	GCCGAG	1 cut(s)	1606
NmuCI	GTSAC	7 cut(s)	106, 167, 238, 754, 1364, 1438, 1933
NspI	RCATGY	2 cut(s)	340, 1048
PaeR7I	CTCGAG	1 cut(s)	2009
PagI	TCATGA	1 cut(s)	1303
PasI	CCCWGGG	1 cut(s)	1732
PctI	GAATGC	1 cut(s)	1354
PdmI	GAANNNNTTC	2 cut(s)	207, 1419
PfeI	GAWTC	5 cut(s)	229, 560, 1154, 1307, 1619
PkrI	GCNGC	8 cut(s)	157, 442, 766, 1093, 1192, 1384, 1781, 1837
PleI	GAGTC	4 cut(s)	531, 547, 903, 1253
PmaCI	CACGTG	1 cut(s)	1055
PmlI	CACGTG	1 cut(s)	1055
PpsI	GAGTC	4 cut(s)	531, 547, 903, 1253
Ppu21I	YACGTR	1 cut(s)	1055
Psp124BI	GAGCTC	1 cut(s)	1576
Psp6I	CCWGG	1 cut(s)	1731
PspCI	CACGTG	1 cut(s)	1055
PspFI	CCCAGC	1 cut(s)	1352
PspGI	CCWGG	1 cut(s)	1731
PspN4I	GGNNCC	6 cut(s)	403, 464, 1008, 1146, 1462, 1772
PspPI	GGNCC	5 cut(s)	402, 778, 882, 1563, 1771
PspXI	VCTCGAGB	1 cut(s)	2009
PsrI	GAACNNNNNNTAC	2 cut(s)	56, 88
PstI	CTGCAG	1 cut(s)	486
PsuI	RGATCY	3 cut(s)	1006, 1066, 1144
PvuII	CAGCTG	1 cut(s)	1835
RsaI	GTAC	3 cut(s)	629, 849, 1275
RsaNI	GTAC	3 cut(s)	628, 848, 1274
SacI	GAGCTC	1 cut(s)	1576
SaqAI	TTAA	1 cut(s)	929
SatI	GCNGC	8 cut(s)	156, 441, 765, 1092, 1191, 1383, 1780, 1836
Sau3AI	GATC	9 cut(s)	318, 487, 826, 1006, 1066, 1144, 1300, 1763, 2107
Sau96I	GGNCC	5 cut(s)	402, 778, 882, 1563, 1771
SbfI	CCTGCAGG	1 cut(s)	486
SchI	GAGTC	4 cut(s)	531, 547, 904, 1253
ScrFI	CCNGG	1 cut(s)	1733
SdaI	CCTGCAGG	1 cut(s)	486
SduI	GDGCHC	3 cut(s)	37, 1243, 1576
SfaNI	GCATC	7 cut(s)	125, 304, 483, 517, 1129, 1202, 2152
SfcI	CTRYAG	3 cut(s)	243, 482, 735
Sfr274I	CTCGAG	1 cut(s)	2009
SinI	GGWCC	4 cut(s)	402, 778, 882, 1563
SlaI	CTCGAG	1 cut(s)	2009
SmlI	CTYRAG	4 cut(s)	583, 782, 928, 2009
SmoI	CTYRAG	4 cut(s)	583, 782, 928, 2009
Sse8387I	CCTGCAGG	1 cut(s)	486
SsiI	CCGC	4 cut(s)	119, 767, 1566, 1780
SspI	AATATT	1 cut(s)	717
SspMI	CTAG	6 cut(s)	1049, 1449, 1577, 1623, 1748, 1929
SstI	GAGCTC	1 cut(s)	1576
StyD4I	CCNGG	1 cut(s)	1731
StyI	CCWWGG	6 cut(s)	349, 397, 1198, 1555, 1690, 1784
TaaI	ACNGT	9 cut(s)	128, 167, 247, 277, 877, 1214, 1253, 1337, 1885
TaiI	ACGT	2 cut(s)	1057, 2143
TaqI	TCGA	6 cut(s)	232, 409, 618, 1310, 1887, 2010
TatI	WGTACW	1 cut(s)	627
TauI	GCSGC	1 cut(s)	1782
TfiI	GAWTC	5 cut(s)	229, 560, 1154, 1307, 1619
Tru1I	TTAA	1 cut(s)	929
Tru9I	TTAA	1 cut(s)	929
TscAI	CASTG	5 cut(s)	131, 763, 1384, 1488, 2131
TseFI	GTSAC	7 cut(s)	106, 167, 238, 754, 1364, 1438, 1933
TseI	GCWGC	7 cut(s)	155, 440, 764, 1091, 1190, 1382, 1835
Tsp45I	GTSAC	7 cut(s)	106, 167, 238, 754, 1364, 1438, 1933
TspGWI	ACGGA	1 cut(s)	300
TspRI	CASTG	5 cut(s)	131, 763, 1384, 1488, 2131
Vha464I	CTTAAG	1 cut(s)	928
VpaK11BI	GGWCC	4 cut(s)	402, 778, 882, 1563
XagI	CCTNNNNNAGG	1 cut(s)	1873
XapI	RAATTY	4 cut(s)	419, 1511, 1680, 2027
XceI	RCATGY	2 cut(s)	340, 1048
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1324
XhoI	CTCGAG	1 cut(s)	2009
XmiI	GTMKAC	1 cut(s)	1060
XmnI	GAANNNNTTC	2 cut(s)	207, 1419
XspI	CTAG	6 cut(s)	1049, 1449, 1577, 1623, 1748, 1929

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.