Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000008

Orthogroup ID

OG0002518

Physical Location & Seq

Genomic Coordinates Reverse (-)

16244 .. 21613

Gene Structure 5370 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RorugPtG0000800.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4494 bp

ATGAGGGACGGTCATAATAAGGTTTACAAGTCGTTTTCAGATGGATTTAACGCAGACTTTGATGGGGATCAAATGGCTGTTCATGTACCTTTATCTTTGGAGGCTCAAGCAGAGGCCCGTTTACTTATGTTTTCTCATATGAATCTCTTGTCTCCAGCTATTGGGGATCCTATTTCCGTACCAACCCAAGATATGCTTATCGGGCTCTATGTATTAACGAAACCTTTTTTTTGTAATTCCTATGATGCAATTGGAGCTTATCGGCAGAAAAGAGTCAATTTAGATAGCCCCTTGTGGCTCCGTATAAAAAAAGAAGTTCTTTGTATATATGTTCGAACCACAGTTGGTCATATTTCTCTTTATCGAGAAATCGAAGAAGCTATCCAAGGGTTTTGCCGAGCCTACTCATCGAGAAAAGGAAGTTTTCCAATCACTGACTCAAACCCATTGTCGAACCCCACTCAGCGGAATAGGGAGGTACTTATGGCAGAACGGGCCGGTCTGGTTTTTCACAATAAAGTGATCGATGGAACCGCTATTAAACGACTTATTAGTAGATTAATAGATCACTTCGGAATGGCATATACATCACACATCCTAGATCAAGTAAAGACTCTGGGGTTTCGACAAGCCACTGCTACATCCATTTCATTAGGAATTGATGATCTTTTAACAATACCTTCTAAGGGGTGGCTAGTCCAAGATGCCGAACAACAAACTAGAGGAAATGCATCTCAAGTACACCAATTAGTAGGTATGAGAGGGTTAATGTCGGATCCACAAGGACAAATGATTGATTTACCTATTCAAAGCAATTTACGTGAAGGGCTGTCTTTAACAGAATATATCATTTCTTGTTACGGAGCCCGCAAGGGCGTTGTGGATACTGCTGTACGAACGTCGGATGCTGGATATCTTACACGCAGACTTGTTGAAGTAGTTCAACACATTGTTGTACGTAGAACAGATTGTGGCACCGTCCGAGGTATTTCTGTGAGTCCTCGAAATGGGATGATGCCGGAAAGAATTTTTATCCAAACATTGATTGGCCGTGTATTAGCAGACGATATATATATAGGACCGCGATGCATTGCCGTTAGAAATCAAGATATTGGAATTGGACTTGTCAATCGATTCATAACCTTTCAAACACAACCAATACCTATTCGAACCCCCTTTACTTGTAAGAGTACATCTTGGATCTGCCGATTATGTTATGGCCGGAGCCCTACTCATGGCGACCTGGTCGAGTTGGGGGAAGCTGTAGGCATTATTGCGGGTCAATCTATTGGAGAACCGGGCACTCAATTAACATTAAGAACTTTTCATACCGGTGGAGTATTCACAGGGGGTACTGCAGAACATGTACGAGCCCCTTCTAATGGAAAAATAAAATTCAATGAGGATTTGGTTCATCCCACACGTACACGTCATGGGCATCCTGCTTTTCTATGTTATATAGACTTGTATGTAACTATTGAAAGTGAAAATATTATACATAACGTGACTATTCCACCCAAAAGTTTGATTTTAGTGCAAAATGACCAATACGTAGAATCAGAGCAAGTGGTTGCTGAGATTCGCGCGGGAGCATACACTTTTAATTTTAAAGAGAGGGTTCGAAAACATATTTATTCTGACTCAGAGGGAGAAATGCACTGGAGTACCGATGTATATCATGCGCCAGAATTTACATATAGTAATGTACATCTTTTACCCAAAACAAGTCATTTGTGGATATTATCAGGAGGTTCGTGCAGATTCAGTGCAGTCCCCCCTTCACTCCACAAGGATCAAGATCAAACGAACGTTCATTCTCTTTCGGCTGAAGGACGATATTTTTCTAGTCTTTCAGTAAATAATGATCAAGTGAAACACAAATTCGTTGGTTTGAATCTTTCTGGTAAAAAAGAAAGCTGTATTCCTGATTATTCAGAATTGAATCGAATCATATATACGAGTCATTGTAATCTCATATTTCCTCCTATTCGCCATGATAATTTTTTATTGACAAAGAGGCGAAGAAATAGATTCATCATTCCGTTCCAATCGATTCAAGAACAAGAGAAAGAACGGATGCCCCGTCCCGATATTTCGATTGAAATACCGATAAATGGTATTTTTCGTAGAAATAGTATTCTTGCTTATTTCGACGATCCTCAATACAGAAGAAAGAGTTCAGGAATTACTAAATATGGAACTGTAGGGTTGCATTCAATCCTCAAAAAAGAGGATTTAATTGAGTACCGAGGAGTCAAAGAATTTAAGCCAAAATACCAAACGAAAGTAGATCGATTTTTTTTCATTCCTGAGGAAGTGCATATTTTACCCGAGTCTTCTTCCATAATGGTACGGAACAATAGTATCATTGGGATAGATACACGAATCACTTTAAATACAAGAAGCAGAGTAGGTGGCTTGGTCCGAATAGAGAGAAAAAAAAAAAGGATTGAACTTAAAATCTTTTCTGGAGATATCCATTTTCCTGGAGAGATGGATAAGATATTCCGACACAACGGCATCTTGATACCGCCAGGAACAAATTCTAAGGAATCAAAAAAGCGGAACAATTGGATTTATGTCCAATGGATCACACCCACCAAGAAAAAGTATTTTGTTTTGGTTCGACCCGTAATCATATATGAAATTGCGGACGGTATAAATTTAGCAACACTTTTCCCCCAGGATCCATTGCGGGAAAGGGATAATCTAGAACTTCGAGTTGTAAATTATATACTTTATGGAAATGGCAAACCAATTCGGGGAATTTCTGGCACAAGTATTCAATTAGTTCGGACCTGTTTAATCTTGAACTGGGACAAAAACAAAAAAAGTTCTTCTATCGAAGAGGCCCACGCTTCCTTTGTTGAAGTAAGTGCGAATGGTCTGATTCAAGATTTCCTCAGAATCAACTTAGTGAAATCCCATACTTCGTATATCCGAAAAAGGAATGATCCATTAGGTTCAGGATTGATCTCTGATAATAGGTCGGATCGTACCAATATCAATCCATTTTATTCTATTTATTCCAAGGAAAGAATTCAACAATCGCTTAGACAAAATCAAGGAACTTTTCGTACGTTGTTGAATAGAAATAAGGAATCCCAATCTTTGATAATTTTGTCATCATATAATTGTTTTCAAATGAGTCCATTCAACGATGTAAAATATTACGATGGATTCAACGATGTAAAATATTACGATGGGATAAAAGAATCAATTAAAAGAGATAGGGATTCCTTAATTCAAATTACAAATTTGTTAGGCCCCTTAGGAACAGCCTCTCAAATTGATCTTTTTTATTCGTTTTACCATTTACTAACTCATAATCATATTTCGGTAACTAAATATTTCTATTTGCAACTCGACAATTTAAAACAGACTTTTCAAGTATTTAAGTATTATTTAATGGATGAAAACGGGAGAATTTCGAATTCCGATCCGTGCAGTAGCATCCTTTTGAATCCGCTTAACTTGAATTGGCATTTTCTCGACCATAATTATTGTGAGGAAACATCCACAATAATTAGTCTCGGGCAGTTTATTTGTGAAAATCTATGTATAGCCAAAAAAGGATCGCCCCTAAAATCGGGTCAAGTTATAGTCGTTCAACTTGACTCTTTAGTAATAAGATCGGCGAAGCCTTATTTAGCTACTCCAGGAGCAACTGTTCATGGACATTATGGAGAAACCCTTTCCGAAGGAGATACATTAGTTACATTTATATATGAAAAATCTCGATCTGGTGATATAACGCAAGGTCTTCCAAAAGTAGAACAGGTTTTAGAGGTACGTTCGATTGATTCAATATCGATGAACCTAGAAAAGAGAGTTGAGGGTTGGAATGAGTGTATAACAAGAATTCTTGGAATTCCTTGGGGATTCTTGATTGGCGCTGAGCTAACTATAGCGCAAAGTCGTATCTCTTTGGTTAATAAGATCCAAAAAGTTTATAGATCCCAGGGGGTACAGATCCATAATAGGCATATCGAAATCATTGTACGTCAAATAACATCAAAAGTGTTGGTTTCAGAAGATGGAATGTCTAATGTTTTTTCACCCGGAGAACTAATTGGATTGCTGCGAGCTGAACGAACGGGGCGCGCTTTAGAAGAAGCGATTTGTTACCGAGCCATATTATTAGGAATAACGAAAGCATCTCTAAATACTCAAAGTTTCATATCCGAAGCAAGTTTTCAAGAAACTGCTCGAGTTTTAGCAAAAGCAGCCCTCCGGGGTCGTATCGATTGGTTGAAAGGTCTGAAAGAGAACGTTGTTCTAGGAGGGATGATACCCGTTGGTACCGGATTCAAAGGATTCGTGCACCGTTCAAGGCAACATAACAACATTTCTTTGGAAACCAAAAATAAGAGTTTATTCGAGGGGGAAATGAGAGATATTTTGGTCCACCACAGAGAATTATTTGATTTTTGCATTTCAAAAAATTTACAGGATACATCAGAACAATCTTTTTTCGGATTTAATGATTCCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2

Protein Analysis

1497

Amino Acids

169.79

Weight (kDa)

9.15

Isoelectric Point (pI)

43.87

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RNA_pol_Rpb1_2	PF00623	10 - 43	4.9e-09	RNA polymerase Rpb1, domain 2
RNA_pol_Rpb1_3	PF04983	48 - 223	1.8e-16	RNA polymerase Rpb1, domain 3
RNA_pol_Rpb1_5	PF04998	276 - 469	1.5e-39	RNA polymerase Rpb1, domain 5
RNA_pol_Rpb1_5	PF04998	1301 - 1388	1.9e-07	RNA polymerase Rpb1, domain 5

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002518)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G09190
fragaria_vesca	FvH4_1g08040
malus_domestica	MD02G1085000.v1.1
prunus_persica	Prupe.7G204600_v2.0.a1
pyrus_communis	pycom02g06780 pycom02g20400
rosa_chinensis	RchiOBHm_Chr1g0349371 RchiOBHm_Chr1g0365421 RchiOBHm_Chr2g0094071 RchiOBHm_Chr2g0100501 RchiOBHm_Chr2g0145241 RchiOBHm_Chr5g0027681
rosa_laevigata	RLG00000016453 RLG00000016978 RLG00000028565
rosa_multiflora	Rmu_sc0000528.1_g000021 Rmu_sc0001942.1_g000078 Rmu_sc0001989.1_g000005 Rmu_sc0001989.1_g000006 Rmu_sc0002834.1_g000001 Rmu_sc0010460.1_g000009 Rmu_sc0015757.1_g000001 Rmu_sc0023774.1_g000001 Rmu_ssc0000469.1_g000004
rosa_roxburghii	Rroxscaffold_1G00065660 Rroxscaffold_2G00147500
rosa_rugosa	Rorug01G0191200 Rorug01G0245800 Rorug01G0404500 Rorug02G0040700 Rorug02G0042900 Rorug02G0088700 Rorug02G0306900 Rorug02G0425200 RorugPtG0000800
rosa_samantha	Rh1BG121500 Rh2BG141500 Rh2BG455200 Rh2CG090800 Rh2CG143700 Rh2DG465200

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	4301
AccB1I	GGYRCC	2 cut(s)	974, 4301
AccB7I	CCANNNNNTGG	1 cut(s)	161
AccII	CGCG	4 cut(s)	1084, 1584, 1586, 4104
AclI	AACGTT	2 cut(s)	1809, 4272
AcoI	YGGCCR	2 cut(s)	1048, 1219
AcuI	CTGAAG	1 cut(s)	1848
AfiI	CCNNNNNNNGG	9 cut(s)	161, 465, 686, 1007, 1235, 1382, 2114, 2762, 3589
AflIII	ACRYGT	3 cut(s)	1363, 1421, 1427
AgeI	ACCGGT	1 cut(s)	1331
AjiI	CACGTC	1 cut(s)	1430
AjnI	CCWGG	6 cut(s)	1242, 2485, 2533, 2682, 3658, 3960
AloI	GAACNNNNNNTCC	2 cut(s)	3654, 3686
AluBI	AGCT	8 cut(s)	158, 257, 380, 1262, 1917, 3653, 3901, 4088
AluI	AGCT	8 cut(s)	158, 257, 380, 1262, 1917, 3653, 3901, 4088
Alw21I	GWGCWC	1 cut(s)	4326
Alw26I	GTCTC	2 cut(s)	156, 3536
Alw44I	GTGCAC	1 cut(s)	4322
Ama87I	CYCGRG	3 cut(s)	2330, 3533, 4209
AoxI	GGCC	6 cut(s)	114, 495, 1048, 1219, 2850, 3265
ApaLI	GTGCAC	1 cut(s)	4322
ApeKI	GCWGC	2 cut(s)	4081, 4226
ArsI	GACNNNNNNTTYG	2 cut(s)	434, 466
AseI	ATTAAT	1 cut(s)	560
AsiGI	ACCGGT	1 cut(s)	1331
Asp700I	GAANNNNTTC	4 cut(s)	939, 2176, 2542, 3039
Asp718I	GGTACC	1 cut(s)	4301
AspLEI	GCGC	6 cut(s)	1586, 1684, 3896, 3913, 4104, 4106
AspS9I	GGNCC	8 cut(s)	115, 495, 1079, 2422, 2796, 2851, 3266, 4405
AsuC2I	CCSGG	3 cut(s)	1299, 4062, 4235
AsuHPI	GGTGA	2 cut(s)	3758, 4050
AsuII	TTCGAA	4 cut(s)	334, 1168, 1621, 3431
AvaI	CYCGRG	3 cut(s)	2330, 3533, 4209
AvaII	GGWCC	4 cut(s)	1079, 2422, 2796, 4405
AxyI	CCTNAGG	2 cut(s)	2310, 3271
BaeGI	GKGCMC	2 cut(s)	1304, 4326
BaeI	ACNNNNGTAYC	8 cut(s)	876, 909, 1648, 1681, 2347, 2380, 3699, 3732
BamHI	GGATCC	3 cut(s)	166, 775, 2686
BanI	GGYRCC	2 cut(s)	974, 4301
BanII	GRGCYC	4 cut(s)	207, 868, 1229, 1375
BbsI	GAAGAC	2 cut(s)	2328, 3755
Bbv12I	GWGCWC	1 cut(s)	4326
BbvI	GCAGC	2 cut(s)	4068, 4238
BccI	CCATC	7 cut(s)	35, 56, 521, 2488, 3170, 3197, 4031
BceAI	ACGGC	3 cut(s)	1035, 1079, 2533
BciT130I	CCWGG	6 cut(s)	1244, 2487, 2535, 2684, 3660, 3962
BciVI	GTATCC	2 cut(s)	877, 4447
BclI	TGATCA	1 cut(s)	1864
BcnI	CCSGG	3 cut(s)	1299, 4062, 4235
BcoDI	GTCTC	2 cut(s)	156, 3536
BfaI	CTAG	7 cut(s)	599, 695, 720, 1845, 2711, 3821, 4280
BfmI	CTRYAG	4 cut(s)	1263, 1356, 2202, 3906
BfoI	RGCGCY	1 cut(s)	3897
BfuI	GTATCC	2 cut(s)	877, 4447
BisI	GCNGC	2 cut(s)	4082, 4227
BlpI	GCTNAGC	1 cut(s)	3897
BlsI	GCNGC	2 cut(s)	4083, 4228
Bme1390I	CCNGG	9 cut(s)	1244, 1299, 2487, 2535, 2684, 3660, 3962, 4062, 4235
Bme18I	GGWCC	4 cut(s)	1079, 2422, 2796, 4405
BmeT110I	CYCGRG	3 cut(s)	2330, 3533, 4209
BmgBI	CACGTC	1 cut(s)	1430
BmgT120I	GGNCC	8 cut(s)	115, 495, 1079, 2422, 2796, 2851, 3266, 4405
BmrFI	CCNGG	9 cut(s)	1244, 1299, 2487, 2535, 2684, 3660, 3962, 4062, 4235
BmrI	ACTGGG	1 cut(s)	2824
BmuI	ACTGGG	1 cut(s)	2824
BpiI	GAAGAC	2 cut(s)	2328, 3755
BplI	GAGNNNNNCTC	2 cut(s)	1216, 1248
BpmI	CTGGAG	5 cut(s)	138, 1681, 2490, 2508, 3642
Bpu1102I	GCTNAGC	1 cut(s)	3897
Bpu14I	TTCGAA	4 cut(s)	334, 1168, 1621, 3431
BpuEI	CTTGAG	2 cut(s)	90, 720
BpuMI	CCSGG	3 cut(s)	1299, 4062, 4235
Bsa29I	ATCGAT	6 cut(s)	525, 1132, 2051, 2293, 3812, 4245
BsaAI	YACGTR	4 cut(s)	821, 959, 1424, 1552
BsaBI	GATNNNNATC	3 cut(s)	66, 1674, 1797
BsaJI	CCNNGG	9 cut(s)	385, 982, 2248, 2682, 3030, 3875, 3960, 3961, 4234
BsaWI	WCCGGW	2 cut(s)	1331, 4304
BsaXI	ACNNNNNCTCC	2 cut(s)	3654, 3684
Bsc4I	CCNNNNNNNGG	9 cut(s)	161, 465, 686, 1007, 1235, 1382, 2114, 2762, 3589
Bse118I	RCCGGY	2 cut(s)	497, 1331
Bse1I	ACTGG	2 cut(s)	1664, 2819
Bse21I	CCTNAGG	2 cut(s)	2310, 3271
Bse3DI	GCAATG	2 cut(s)	1089, 2690
Bse8I	GATNNNNATC	3 cut(s)	66, 1674, 1797
BseBI	CCWGG	6 cut(s)	1244, 2487, 2535, 2684, 3660, 3962
BseCI	ATCGAT	6 cut(s)	525, 1132, 2051, 2293, 3812, 4245
BseDI	CCNNGG	9 cut(s)	385, 982, 2248, 2682, 3030, 3875, 3960, 3961, 4234
BseJI	GATNNNNATC	3 cut(s)	66, 1674, 1797
BseLI	CCNNNNNNNGG	9 cut(s)	161, 465, 686, 1007, 1235, 1382, 2114, 2762, 3589
BseMI	GCAATG	2 cut(s)	1089, 2690
BseMII	CTCAG	6 cut(s)	476, 1566, 1656, 2301, 2917, 3888
BseNI	ACTGG	2 cut(s)	1664, 2819
BsePI	GCGCGC	1 cut(s)	4102
BseRI	GAGGAG	1 cut(s)	2265
BseSI	GKGCMC	2 cut(s)	1304, 4326
BseXI	GCAGC	2 cut(s)	4068, 4238
BsgI	GTGCAG	3 cut(s)	1777, 1788, 3466
Bsh1236I	CGCG	4 cut(s)	1084, 1584, 1586, 4104
BshFI	GGCC	6 cut(s)	116, 497, 1050, 1221, 2852, 3267
BshNI	GGYRCC	2 cut(s)	974, 4301
BshTI	ACCGGT	1 cut(s)	1331
BshVI	ATCGAT	6 cut(s)	525, 1132, 2051, 2293, 3812, 4245
BsiHKAI	GWGCWC	1 cut(s)	4326
BsiHKCI	CYCGRG	3 cut(s)	2330, 3533, 4209
BsiSI	CCGG	8 cut(s)	498, 1019, 1222, 1298, 1332, 4062, 4234, 4305
BsiWI	CGTACG	1 cut(s)	3077
BslFI	GGGAC	4 cut(s)	20, 1757, 2070, 2831
BslI	CCNNNNNNNGG	9 cut(s)	161, 465, 686, 1007, 1235, 1382, 2114, 2762, 3589
BsmAI	GTCTC	2 cut(s)	156, 3536
BsmFI	GGGAC	4 cut(s)	20, 1757, 2070, 2831
BsmI	GAATGC	1 cut(s)	2212
BsnI	GGCC	6 cut(s)	116, 497, 1050, 1221, 2852, 3267
BsoBI	CYCGRG	3 cut(s)	2330, 3533, 4209
Bsp119I	TTCGAA	4 cut(s)	334, 1168, 1621, 3431
Bsp1286I	GDGCHC	6 cut(s)	207, 868, 1229, 1304, 1375, 4326
Bsp1407I	TGTACA	1 cut(s)	1705
Bsp1720I	GCTNAGC	1 cut(s)	3897
BspANI	GGCC	6 cut(s)	116, 497, 1050, 1221, 2852, 3267
BspCNI	CTCAG	6 cut(s)	475, 1567, 1655, 2302, 2916, 3889
BspDI	ATCGAT	6 cut(s)	525, 1132, 2051, 2293, 3812, 4245
BspFNI	CGCG	4 cut(s)	1084, 1584, 1586, 4104
BspMAI	CTGCAG	1 cut(s)	1360
BspT104I	TTCGAA	4 cut(s)	334, 1168, 1621, 3431
BspT107I	GGYRCC	2 cut(s)	974, 4301
BsrDI	GCAATG	2 cut(s)	1089, 2690
BsrFI	RCCGGY	2 cut(s)	497, 1331
BsrGI	TGTACA	1 cut(s)	1705
BsrI	ACTGG	2 cut(s)	1664, 2819
BssAI	RCCGGY	2 cut(s)	497, 1331
BssECI	CCNNGG	9 cut(s)	385, 982, 2248, 2682, 3030, 3875, 3960, 3961, 4234
BssHII	GCGCGC	1 cut(s)	4102
BssT1I	CCWWGG	3 cut(s)	385, 3030, 3875
Bst2UI	CCWGG	6 cut(s)	1244, 2487, 2535, 2684, 3660, 3962
Bst4CI	ACNGT	7 cut(s)	11, 343, 979, 2203, 2657, 3670, 4328
Bst6I	CTCTTC	1 cut(s)	2841
BstAUI	TGTACA	1 cut(s)	1705
BstBAI	YACGTR	4 cut(s)	821, 959, 1424, 1552
BstBI	TTCGAA	4 cut(s)	334, 1168, 1621, 3431
BstC8I	GCNNGC	3 cut(s)	868, 4086, 4104
BstFNI	CGCG	4 cut(s)	1084, 1584, 1586, 4104
BstH2I	RGCGCY	1 cut(s)	3897
BstHHI	GCGC	6 cut(s)	1586, 1684, 3896, 3913, 4104, 4106
BstMAI	GTCTC	2 cut(s)	156, 3536
BstMWI	GCNNNNNNNGC	4 cut(s)	202, 254, 494, 4226
BstNI	CCWGG	6 cut(s)	1244, 2487, 2535, 2684, 3660, 3962
BstNSI	RCATGY	1 cut(s)	1367
BstSCI	CCNGG	9 cut(s)	1242, 1297, 2485, 2533, 2682, 3658, 3960, 4060, 4233
BstSFI	CTRYAG	4 cut(s)	1263, 1356, 2202, 3906
BstSLI	GKGCMC	2 cut(s)	1304, 4326
BstSNI	TACGTA	2 cut(s)	959, 1552
BstUI	CGCG	4 cut(s)	1084, 1584, 1586, 4104
BstV1I	GCAGC	2 cut(s)	4068, 4238
BstV2I	GAAGAC	2 cut(s)	2328, 3755
BstX2I	RGATCY	7 cut(s)	166, 775, 1200, 2686, 3939, 3956, 3972
BstXI	CCANNNNNNTGG	1 cut(s)	2486
BstYI	RGATCY	7 cut(s)	166, 775, 1200, 2686, 3939, 3956, 3972
Bsu15I	ATCGAT	6 cut(s)	525, 1132, 2051, 2293, 3812, 4245
Bsu36I	CCTNAGG	2 cut(s)	2310, 3271
BsuI	GTATCC	2 cut(s)	877, 4447
BsuRI	GGCC	6 cut(s)	116, 497, 1050, 1221, 2852, 3267
BsuTUI	ATCGAT	6 cut(s)	525, 1132, 2051, 2293, 3812, 4245
BtgZI	GCGATG	1 cut(s)	1099
BtrI	CACGTC	1 cut(s)	1430
BtsI	GCAGTG	1 cut(s)	633
BtsIMutI	CAGTG	4 cut(s)	432, 633, 1657, 1771
Cac8I	GCNNGC	3 cut(s)	868, 4086, 4104
CfoI	GCGC	6 cut(s)	1586, 1684, 3896, 3913, 4104, 4106
Cfr10I	RCCGGY	2 cut(s)	497, 1331
Cfr13I	GGNCC	8 cut(s)	115, 495, 1079, 2422, 2796, 2851, 3266, 4405
ClaI	ATCGAT	6 cut(s)	525, 1132, 2051, 2293, 3812, 4245
CsiI	ACCWGGT	1 cut(s)	1242
CspAI	ACCGGT	1 cut(s)	1331
CviAII	CATG	7 cut(s)	83, 1235, 1364, 1433, 1679, 1994, 3674
DraI	TTTAAA	3 cut(s)	1609, 2394, 3375
EaeI	YGGCCR	2 cut(s)	1048, 1219
Eam1104I	CTCTTC	1 cut(s)	2841
EarI	CTCTTC	1 cut(s)	2841
Eco105I	TACGTA	2 cut(s)	959, 1552
Eco130I	CCWWGG	3 cut(s)	385, 3030, 3875
Eco24I	GRGCYC	4 cut(s)	207, 868, 1229, 1375
Eco32I	GATATC	2 cut(s)	914, 2476
Eco47I	GGWCC	4 cut(s)	1079, 2422, 2796, 4405
Eco57I	CTGAAG	1 cut(s)	1848
Eco81I	CCTNAGG	2 cut(s)	2310, 3271
Eco88I	CYCGRG	3 cut(s)	2330, 3533, 4209
EcoO109I	RGGNCCY	1 cut(s)	3266
EcoRI	GAATTC	4 cut(s)	3039, 3433, 3861, 3870
EcoRII	CCWGG	6 cut(s)	1242, 2485, 2533, 2682, 3658, 3960
EcoRV	GATATC	2 cut(s)	914, 2476
EcoT14I	CCWWGG	3 cut(s)	385, 3030, 3875
EcoT22I	ATGCAT	2 cut(s)	733, 1091
EcoT38I	GRGCYC	4 cut(s)	207, 868, 1229, 1375
ErhI	CCWWGG	3 cut(s)	385, 3030, 3875
FaeI	CATG	7 cut(s)	86, 1238, 1367, 1436, 1682, 1997, 3677
FalI	AAGNNNNNCTT	4 cut(s)	180, 212, 303, 335
FaqI	GGGAC	4 cut(s)	20, 1757, 2070, 2831
FatI	CATG	7 cut(s)	82, 1234, 1363, 1432, 1678, 1993, 3673
FauI	CCCGC	4 cut(s)	875, 1270, 1579, 2688
FauNDI	CATATG	1 cut(s)	138
FbaI	TGATCA	1 cut(s)	1864
Fnu4HI	GCNGC	2 cut(s)	4082, 4227
FriOI	GRGCYC	4 cut(s)	207, 868, 1229, 1375
Fsp4HI	GCNGC	2 cut(s)	4082, 4227
FspBI	CTAG	7 cut(s)	599, 695, 720, 1845, 2711, 3821, 4280
GlaI	GCGC	6 cut(s)	1585, 1683, 3895, 3912, 4103, 4105
GluI	GCNGC	2 cut(s)	4082, 4227
GsuI	CTGGAG	5 cut(s)	138, 1681, 2490, 2508, 3642
HaeII	RGCGCY	1 cut(s)	3897
HaeIII	GGCC	6 cut(s)	116, 497, 1050, 1221, 2852, 3267
HapII	CCGG	8 cut(s)	498, 1019, 1222, 1298, 1332, 4062, 4234, 4305
HhaI	GCGC	6 cut(s)	1586, 1684, 3896, 3913, 4104, 4106
Hin1II	CATG	7 cut(s)	86, 1238, 1367, 1436, 1682, 1997, 3677
Hin6I	GCGC	6 cut(s)	1584, 1682, 3894, 3911, 4102, 4104
HinP1I	GCGC	6 cut(s)	1584, 1682, 3894, 3911, 4102, 4104
HpaII	CCGG	8 cut(s)	498, 1019, 1222, 1298, 1332, 4062, 4234, 4305
HphI	GGTGA	2 cut(s)	3758, 4050
Hpy166II	GTNNAC	6 cut(s)	25, 122, 742, 1427, 4324, 4408
Hpy8I	GTNNAC	6 cut(s)	25, 122, 742, 1427, 4324, 4408
Hpy99I	CGWCG	2 cut(s)	904, 2156
HpyAV	CCTTC	6 cut(s)	690, 818, 1386, 1788, 1823, 3695
HpyCH4III	ACNGT	7 cut(s)	11, 343, 979, 2203, 2657, 3670, 4328
HpyF10VI	GCNNNNNNNGC	4 cut(s)	202, 254, 494, 4226
Hsp92II	CATG	7 cut(s)	86, 1238, 1367, 1436, 1682, 1997, 3677
HspAI	GCGC	6 cut(s)	1584, 1682, 3894, 3911, 4102, 4104
KpnI	GGTACC	1 cut(s)	4305
Ksp22I	TGATCA	1 cut(s)	1864
LmnI	GCTCC	6 cut(s)	254, 303, 863, 1224, 1589, 3662
Lsp1109I	GCAGC	2 cut(s)	4068, 4238
MabI	ACCWGGT	1 cut(s)	1242
MaeI	CTAG	7 cut(s)	599, 695, 720, 1845, 2711, 3821, 4280
MaeIII	GTNAC	6 cut(s)	857, 1471, 1504, 3340, 3715, 4124
MfeI	CAATTG	2 cut(s)	249, 2569
MflI	RGATCY	7 cut(s)	166, 775, 1200, 2686, 3939, 3956, 3972
MhlI	GDGCHC	6 cut(s)	207, 868, 1229, 1304, 1375, 4326
MmeI	TCCRAC	5 cut(s)	753, 882, 2533, 2970, 3821
Mph1103I	ATGCAT	2 cut(s)	733, 1091
MroXI	GAANNNNTTC	4 cut(s)	939, 2176, 2542, 3039
MslI	CAYNNNNRTG	1 cut(s)	1332
MspA1I	CMGCKG	1 cut(s)	466
MspI	CCGG	8 cut(s)	498, 1019, 1222, 1298, 1332, 4062, 4234, 4305
MspR9I	CCNGG	9 cut(s)	1244, 1299, 2487, 2535, 2684, 3660, 3962, 4062, 4235
MunI	CAATTG	2 cut(s)	249, 2569
Mva1269I	GAATGC	1 cut(s)	2212
MvaI	CCWGG	6 cut(s)	1244, 2487, 2535, 2684, 3660, 3962
MvnI	CGCG	4 cut(s)	1084, 1584, 1586, 4104
MwoI	GCNNNNNNNGC	4 cut(s)	202, 254, 494, 4226
NciI	CCSGG	3 cut(s)	1299, 4062, 4235
NdeI	CATATG	1 cut(s)	138
NlaIII	CATG	7 cut(s)	86, 1238, 1367, 1436, 1682, 1997, 3677
NmeAIII	GCCGAG	1 cut(s)	422
NmuCI	GTSAC	1 cut(s)	1504
NsiI	ATGCAT	2 cut(s)	733, 1091
NspI	RCATGY	1 cut(s)	1367
NspV	TTCGAA	4 cut(s)	334, 1168, 1621, 3431
PaeR7I	CTCGAG	1 cut(s)	4209
PasI	CCCWGGG	1 cut(s)	3961
PauI	GCGCGC	1 cut(s)	4102
PciI	ACATGT	1 cut(s)	1363
PcsI	WCGNNNNNNNCGW	1 cut(s)	2080
PctI	GAATGC	1 cut(s)	2212
PdmI	GAANNNNTTC	4 cut(s)	939, 2176, 2542, 3039
Pfl23II	CGTACG	1 cut(s)	3077
PflFI	GACNNNGTC	1 cut(s)	1244
PflMI	CCANNNNNTGG	1 cut(s)	161
PfoI	TCCNGGA	2 cut(s)	2485, 3658
PinAI	ACCGGT	1 cut(s)	1331
PkrI	GCNGC	2 cut(s)	4083, 4228
Ppu21I	YACGTR	4 cut(s)	821, 959, 1424, 1552
PscI	ACATGT	1 cut(s)	1363
PshBI	ATTAAT	1 cut(s)	560
Psp1406I	AACGTT	2 cut(s)	1809, 4272
Psp6I	CCWGG	6 cut(s)	1242, 2485, 2533, 2682, 3658, 3960
PspGI	CCWGG	6 cut(s)	1242, 2485, 2533, 2682, 3658, 3960
PspLI	CGTACG	1 cut(s)	3077
PspPI	GGNCC	8 cut(s)	115, 495, 1079, 2422, 2796, 2851, 3266, 4405
PspXI	VCTCGAGB	1 cut(s)	4209
PsrI	GAACNNNNNNTAC	6 cut(s)	1162, 1194, 1312, 1344, 3061, 3093
PstI	CTGCAG	1 cut(s)	1360
PsuI	RGATCY	7 cut(s)	166, 775, 1200, 2686, 3939, 3956, 3972
PsyI	GACNNNGTC	1 cut(s)	1244
PteI	GCGCGC	1 cut(s)	4102
RseI	CAYNNNNRTG	1 cut(s)	1332
SatI	GCNGC	2 cut(s)	4082, 4227
Sau96I	GGNCC	8 cut(s)	115, 495, 1079, 2422, 2796, 2851, 3266, 4405
ScrFI	CCNGG	9 cut(s)	1244, 1299, 2487, 2535, 2684, 3660, 3962, 4062, 4235
SduI	GDGCHC	6 cut(s)	207, 868, 1229, 1304, 1375, 4326
SexAI	ACCWGGT	1 cut(s)	1242
SfcI	CTRYAG	4 cut(s)	1263, 1356, 2202, 3906
Sfr274I	CTCGAG	1 cut(s)	4209
SfuI	TTCGAA	4 cut(s)	334, 1168, 1621, 3431
SinI	GGWCC	4 cut(s)	1079, 2422, 2796, 4405
SlaI	CTCGAG	1 cut(s)	4209
SmiMI	CAYNNNNRTG	1 cut(s)	1332
SmlI	CTYRAG	3 cut(s)	105, 735, 4209
SmoI	CTYRAG	3 cut(s)	105, 735, 4209
SnaBI	TACGTA	2 cut(s)	959, 1552
SspI	AATATT	4 cut(s)	1492, 3170, 3197, 3350
SspMI	CTAG	7 cut(s)	599, 695, 720, 1845, 2711, 3821, 4280
StyD4I	CCNGG	9 cut(s)	1242, 1297, 2485, 2533, 2682, 3658, 3960, 4060, 4233
StyI	CCWWGG	3 cut(s)	385, 3030, 3875
TaaI	ACNGT	7 cut(s)	11, 343, 979, 2203, 2657, 3670, 4328
TatI	WGTACW	3 cut(s)	739, 1190, 1705
TscAI	CASTG	4 cut(s)	439, 640, 1664, 1771
TseFI	GTSAC	1 cut(s)	1504
TseI	GCWGC	2 cut(s)	4081, 4226
Tsp45I	GTSAC	1 cut(s)	1504
TspGWI	ACGGA	7 cut(s)	166, 290, 876, 2031, 2089, 2368, 3432
TspRI	CASTG	4 cut(s)	439, 640, 1664, 1771
Tth111I	GACNNNGTC	1 cut(s)	1244
Van91I	CCANNNNNTGG	1 cut(s)	161
VneI	GTGCAC	1 cut(s)	4322
VpaK11BI	GGWCC	4 cut(s)	1079, 2422, 2796, 4405
VspI	ATTAAT	1 cut(s)	560
XbaI	TCTAGA	1 cut(s)	2710
XceI	RCATGY	1 cut(s)	1367
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1043
XhoI	CTCGAG	1 cut(s)	4209
XmnI	GAANNNNTTC	4 cut(s)	939, 2176, 2542, 3039
XspI	CTAG	7 cut(s)	599, 695, 720, 1845, 2711, 3821, 4280
Zsp2I	ATGCAT	2 cut(s)	733, 1091

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.