pycom02g20400

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0002518
Physical Location & Seq
Forward (+)
18519369 .. 18519617
249 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g20400.1

Sequence Viewer

Length: 249 bp
ATGCCTGTGGAGCCTCATGCTGGTGCTTGGGGTGCACTACTTTCAGCCTGTAAGCTAAACTGCAATGCTGAGCTAGGAGAGTTAGTTGCTCATCAACTTTTTGAGCTTGAACCCCAAAATCCTGGTAATTATGTGCTCTTGTCCGATATCTATGCAGAAACAGGCCGCTGGTTTGATGTTTCTGCAGTGAGGACCAAAATGGAAGAGCAAGGGATTAAAAAGATACCCGGTATCAGTTATTTAGTTTAA

Protein Analysis

83

Amino Acids

9.03

Weight (kDa)

4.95

Isoelectric Point (pI)

38.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
E_motif PF20431 22 - 80 2.2e-23 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 166
AfiI CCNNNNNNNGG 1 cut(s) 20
AgsI TTSAA 1 cut(s) 110
AjnI CCWGG 1 cut(s) 121
AluBI AGCT 3 cut(s) 55, 73, 106
AluI AGCT 3 cut(s) 55, 73, 106
Alw21I GWGCWC 2 cut(s) 37, 138
Alw44I GTGCAC 1 cut(s) 33
AoxI GGCC 1 cut(s) 163
ApaLI GTGCAC 1 cut(s) 33
AspS9I GGNCC 1 cut(s) 192
AsuC2I CCSGG 1 cut(s) 228
AvaII GGWCC 1 cut(s) 192
BaeGI GKGCMC 1 cut(s) 37
Bbv12I GWGCWC 2 cut(s) 37, 138
BcgI CGANNNNNNTGC 2 cut(s) 134, 168
BciT130I CCWGG 1 cut(s) 123
BcnI CCSGG 1 cut(s) 228
BfaI CTAG 1 cut(s) 74
BfmI CTRYAG 1 cut(s) 183
BisI GCNGC 1 cut(s) 166
BlpI GCTNAGC 1 cut(s) 69
BlsI GCNGC 1 cut(s) 167
Bme1390I CCNGG 2 cut(s) 123, 228
Bme18I GGWCC 1 cut(s) 192
BmgT120I GGNCC 1 cut(s) 192
BmiI GGNNCC 1 cut(s) 12
BmrFI CCNGG 2 cut(s) 123, 228
Bpu1102I GCTNAGC 1 cut(s) 69
BpuMI CCSGG 1 cut(s) 228
BsaXI ACNNNNNCTCC 2 cut(s) 69, 99
Bsc4I CCNNNNNNNGG 1 cut(s) 20
Bse3DI GCAATG 1 cut(s) 70
BseBI CCWGG 1 cut(s) 123
BseLI CCNNNNNNNGG 1 cut(s) 20
BseMI GCAATG 1 cut(s) 70
BseMII CTCAG 1 cut(s) 60
BseSI GKGCMC 1 cut(s) 37
BshFI GGCC 1 cut(s) 165
BsiHKAI GWGCWC 2 cut(s) 37, 138
BsiSI CCGG 1 cut(s) 228
BslI CCNNNNNNNGG 1 cut(s) 20
BsnI GGCC 1 cut(s) 165
Bsp1286I GDGCHC 2 cut(s) 37, 138
Bsp1720I GCTNAGC 1 cut(s) 69
BspACI CCGC 1 cut(s) 166
BspANI GGCC 1 cut(s) 165
BspCNI CTCAG 1 cut(s) 61
BspLI GGNNCC 1 cut(s) 12
BspMAI CTGCAG 1 cut(s) 187
BspQI GCTCTTC 1 cut(s) 198
BsrDI GCAATG 1 cut(s) 70
Bst2UI CCWGG 1 cut(s) 123
Bst6I CTCTTC 1 cut(s) 198
BstDEI CTNAG 1 cut(s) 69
BstMWI GCNNNNNNNGC 2 cut(s) 10, 32
BstNI CCWGG 1 cut(s) 123
BstSCI CCNGG 2 cut(s) 121, 226
BstSFI CTRYAG 1 cut(s) 183
BstSLI GKGCMC 1 cut(s) 37
BstXI CCANNNNNNTGG 1 cut(s) 122
BsuRI GGCC 1 cut(s) 165
BtsI GCAGTG 1 cut(s) 192
BtsIMutI CAGTG 1 cut(s) 192
Cfr13I GGNCC 1 cut(s) 192
CviAII CATG 1 cut(s) 17
CviJI RGCY 6 cut(s) 13, 47, 55, 73, 106, 165
CviKI_1 RGCY 6 cut(s) 13, 47, 55, 73, 106, 165
DdeI CTNAG 1 cut(s) 69
Eam1104I CTCTTC 1 cut(s) 198
EarI CTCTTC 1 cut(s) 198
Eco32I GATATC 1 cut(s) 148
Eco47I GGWCC 1 cut(s) 192
EcoRII CCWGG 1 cut(s) 121
EcoRV GATATC 1 cut(s) 148
FaeI CATG 1 cut(s) 20
FaiI YATR 3 cut(s) 18, 132, 153
FatI CATG 1 cut(s) 16
Fnu4HI GCNGC 1 cut(s) 166
Fsp4HI GCNGC 1 cut(s) 166
FspBI CTAG 1 cut(s) 74
GluI GCNGC 1 cut(s) 166
HaeIII GGCC 1 cut(s) 165
HapII CCGG 1 cut(s) 228
Hin1II CATG 1 cut(s) 20
HpaII CCGG 1 cut(s) 228
Hpy166II GTNNAC 1 cut(s) 35
Hpy188I TCNGA 1 cut(s) 145
Hpy8I GTNNAC 1 cut(s) 35
HpyCH4V TGCA 4 cut(s) 35, 63, 155, 185
HpyF10VI GCNNNNNNNGC 2 cut(s) 10, 32
HpyF3I CTNAG 1 cut(s) 69
Hsp92II CATG 1 cut(s) 20
LguI GCTCTTC 1 cut(s) 198
LmnI GCTCC 1 cut(s) 10
LpnPI CCDG 8 cut(s) 6, 18, 61, 108, 135, 147, 154, 241
MaeI CTAG 1 cut(s) 74
MboII GAAGA 1 cut(s) 215
MhlI GDGCHC 2 cut(s) 37, 138
MluCI AATT 1 cut(s) 127
MnlI CCTC 2 cut(s) 24, 183
MseI TTAA 2 cut(s) 216, 247
MslI CAYNNNNRTG 1 cut(s) 21
MspA1I CMGCKG 1 cut(s) 168
MspI CCGG 1 cut(s) 228
MspR9I CCNGG 2 cut(s) 123, 228
MvaI CCWGG 1 cut(s) 123
MwoI GCNNNNNNNGC 2 cut(s) 10, 32
NciI CCSGG 1 cut(s) 228
NlaIII CATG 1 cut(s) 20
NlaIV GGNNCC 1 cut(s) 12
PciSI GCTCTTC 1 cut(s) 198
PkrI GCNGC 1 cut(s) 167
Psp6I CCWGG 1 cut(s) 121
PspGI CCWGG 1 cut(s) 121
PspN4I GGNNCC 1 cut(s) 12
PspPI GGNCC 1 cut(s) 192
PstI CTGCAG 1 cut(s) 187
RseI CAYNNNNRTG 1 cut(s) 21
SapI GCTCTTC 1 cut(s) 198
SaqAI TTAA 2 cut(s) 216, 247
SatI GCNGC 1 cut(s) 166
Sau96I GGNCC 1 cut(s) 192
ScrFI CCNGG 2 cut(s) 123, 228
SduI GDGCHC 2 cut(s) 37, 138
SetI ASST 3 cut(s) 57, 75, 108
SfcI CTRYAG 1 cut(s) 183
SinI GGWCC 1 cut(s) 192
SmiMI CAYNNNNRTG 1 cut(s) 21
Sse9I AATT 1 cut(s) 127
SsiI CCGC 1 cut(s) 166
SspMI CTAG 1 cut(s) 74
StyD4I CCNGG 2 cut(s) 121, 226
TasI AATT 1 cut(s) 127
TauI GCSGC 1 cut(s) 168
Tru1I TTAA 2 cut(s) 216, 247
Tru9I TTAA 2 cut(s) 216, 247
TscAI CASTG 1 cut(s) 192
TspRI CASTG 1 cut(s) 192
VneI GTGCAC 1 cut(s) 33
VpaK11BI GGWCC 1 cut(s) 192
XspI CTAG 1 cut(s) 74
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.