FvH4_1g08010

phosphatase 2C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0010053
Physical Location & Seq
Forward (+)
4237675 .. 4240811
3137 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g08010.t1

Sequence Viewer

Length: 1416 bp
ATGGAAGACATAGCTCAAGCAGTTGCAGTTCCATATAGGTTAGGCAATCTGATTCATGAGGAATCAGCAGTGAAAACCCAAATGGAAATCACTGGTCTGAAGCTAATATCAAATACATCAGCCTTGTTACCCAATACAAAGCCAAGTTTACCATGTGAGTCCATTTCTTGTGGAAATCAGCAGAGTTACAGAAGCAAAGTTCCACAGGATGAAAACAGAGATCTGATTTCTGGTGAAACCATTAATTGGGGAAGTAAGGAGTGCAAGTTCATGCCTTTGTGCAATCGGGTACTTGGTAGTTCATGCTTTAAGTCTTTATCTCATGATGAGGGTGAGATTTGCAGTGGATCCAATACTTCTGATGCATCCACCATGGTGAATCGGGCGAAGAAAATATGCAGAACAAGCCCCAATGGGAAGGTTTTCGATTTGGATCGTGCACCCTTTTGGGGATACACATCCATCTGTGGAGGAAGACCAGAGATGGAAGATGATGTTGCAGTTGTTCCCAAACTTTTGCAGATTCCTAGTCAAATGTTCATGGATGAATCTGATGACTTGGGCCACAACCCAAGTCAGTATACTGCCCATTTCTTTGGAGTTTATGATGGACATGGGGGTTGTCAGGTTGCTAATTATTGTCGCGAACGTGTGCATTCGGCTTTGGTTGAAGAGATTGAAATTGCCAAATCAGGCTTGAATGAAAGCACTGGTGAATCTTGGCAGGAGCATTGGAAGGAGGCCTTCACCAATTGCTTTATGAAAGTTGATGCCGAGGTTGCAGGAGCTCCCATAAGCACACATTCAAGCAGCACTGGTGCTTCTGAGGCTTCTGTTGATCATATTGCTCCTGAAACTGTCGGTTCTACTGCTGTAGTTGCTGTTGTTTGTCCATCACATATTATAGTAGCAAACTGTGGTGATTCAAGAGCAGTATTGTACCGTGGAAGAGTGGCGATGCCATTGTCAGTAGATCACAAACCAGATAGAGAAGATGAGTATGAAAGGATTGAGGCTGCTGGAGGCAAGGTCATACAGTGGGATGGTTCTCGTGTATTTGGTGTTCTTGCAATGTCACGCTCCATAGGCGACAGATACTTGAAACCATCAATCATACCGGATCCAGAAGTCATGTTTGTTTCTCGAGAAAAGGAAGATGAGTGCCTAATCCTAGCCAGCGACGGGTTGTGGGATGTAATAACAAATGAGGAGGCTTGTGACATTGCGCGGAGGCGGATCCTTGTATGGCATAAGAAATATGGTGATACAGTGGAAAGGGGTGAGGGAGCTGATCCTGCAGCTGAAGCTGCAGCAGTGTACCTCACAAGGCTTGCAATCCAAAAGGGAAGCAAAGACAACATAACTGTTGTAGTGGTAGACCTGAAAGCTTCAAGGAAGTTTAAGAAAAAAACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0001932 GO:0001933 GO:0003674 GO:0003824 GO:0004721 GO:0004722 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005886 GO:0006464 GO:0006469 GO:0006470 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007275 GO:0008150 GO:0008152 GO:0009266 GO:0009314 GO:0009408 GO:0009409 GO:0009414 GO:0009415 GO:0009416 GO:0009628 GO:0009642 GO:0009644 GO:0009719 GO:0009725 GO:0009737 GO:0009787 GO:0009788 GO:0009892 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010035 GO:0010109 GO:0010119 GO:0010205 GO:0010563 GO:0010605 GO:0010646 GO:0010648 GO:0016020 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0019220 GO:0019222 GO:0019538 GO:0019899 GO:0019900 GO:0019901 GO:0022622 GO:0023051 GO:0023057 GO:0031323 GO:0031324 GO:0031399 GO:0031400 GO:0032268 GO:0032269 GO:0032501 GO:0032502 GO:0033673 GO:0033993 GO:0036211 GO:0042221 GO:0042325 GO:0042326 GO:0042548 GO:0042578 GO:0043086 GO:0043155 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043467 GO:0043549 GO:0044092 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045859 GO:0045936 GO:0048364 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0048731 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051174 GO:0051246 GO:0051248 GO:0051338 GO:0051348 GO:0060255 GO:0065007 GO:0065009 GO:0071704 GO:0071944 GO:0080090 GO:0097305 GO:0099402 GO:0140096 GO:1901419 GO:1901420 GO:1901564 GO:1901700 GO:1902456 GO:1905156 GO:1905957 GO:1905958
KEGG Pathways
Metabolic & Signaling
ko04016 ko04075 ko04931 map04016 map04075 map04931
Pfam Domains
Protein Families
PP2C

Protein Analysis

472

Amino Acids

51.59

Weight (kDa)

5.37

Isoelectric Point (pI)

40.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 188 - 454 1.2e-60 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 246
AccI GTMKAC 2 cut(s) 581, 1377
AccII CGCG 2 cut(s) 645, 1228
AciI CCGC 2 cut(s) 1228, 1234
AclWI GGATC 8 cut(s) 342, 355, 441, 1115, 1128, 1231, 1244, 1286
AcuI CTGAAG 2 cut(s) 119, 1323
AfaI GTAC 3 cut(s) 291, 941, 1319
AfiI CCNNNNNNNGG 3 cut(s) 246, 449, 1182
AflIII ACRYGT 1 cut(s) 649
AgsI TTSAA 7 cut(s) 671, 680, 700, 807, 927, 1102, 1392
AleI CACNNNNGTG 1 cut(s) 374
AloI GAACNNNNNNTCC 2 cut(s) 251, 283
AluBI AGCT 7 cut(s) 14, 103, 788, 1289, 1301, 1307, 1388
AluI AGCT 7 cut(s) 14, 103, 788, 1289, 1301, 1307, 1388
Alw21I GWGCWC 2 cut(s) 442, 790
Alw44I GTGCAC 1 cut(s) 438
AlwI GGATC 8 cut(s) 342, 355, 441, 1115, 1128, 1231, 1244, 1286
Ama87I CYCGRG 1 cut(s) 1143
AoxI GGCC 2 cut(s) 562, 741
ApaLI GTGCAC 1 cut(s) 438
ApeKI GCWGC 5 cut(s) 810, 1016, 1298, 1307, 1310
AseI ATTAAT 1 cut(s) 243
Asp700I GAANNNNTTC 1 cut(s) 422
AspLEI GCGC 1 cut(s) 1228
AspS9I GGNCC 1 cut(s) 562
AsuHPI GGTGA 8 cut(s) 245, 344, 388, 725, 739, 932, 1274, 1292
AvaI CYCGRG 1 cut(s) 1143
BaeGI GKGCMC 1 cut(s) 442
BamHI GGATCC 3 cut(s) 347, 1120, 1236
BanII GRGCYC 1 cut(s) 790
BauI CACGAG 1 cut(s) 1050
BbsI GAAGAC 2 cut(s) 12, 481
Bbv12I GWGCWC 2 cut(s) 442, 790
BbvI GCAGC 5 cut(s) 822, 1003, 1294, 1310, 1322
BccI CCATC 6 cut(s) 470, 478, 602, 901, 1037, 1114
BciVI GTATCC 1 cut(s) 446
BclI TGATCA 1 cut(s) 838
BfaI CTAG 2 cut(s) 528, 1172
BfmI CTRYAG 3 cut(s) 873, 1296, 1308
BfuI GTATCC 1 cut(s) 446
BglII AGATCT 1 cut(s) 220
BisI GCNGC 5 cut(s) 811, 1017, 1299, 1308, 1311
BlsI GCNGC 5 cut(s) 812, 1018, 1300, 1309, 1312
BmeT110I CYCGRG 1 cut(s) 1143
BmgT120I GGNCC 1 cut(s) 562
BmiI GGNNCC 3 cut(s) 349, 1122, 1238
BmsI GCATC 4 cut(s) 352, 374, 760, 948
BpiI GAAGAC 2 cut(s) 12, 481
BpmI CTGGAG 1 cut(s) 1041
BsaBI GATNNNNATC 2 cut(s) 432, 457
BsaJI CCNNGG 3 cut(s) 372, 774, 943
BsaWI WCCGGW 1 cut(s) 1117
BsaXI ACNNNNNCTCC 4 cut(s) 251, 281, 1014, 1044
Bsc4I CCNNNNNNNGG 3 cut(s) 246, 449, 1182
Bse1I ACTGG 3 cut(s) 97, 715, 820
Bse3DI GCAATG 2 cut(s) 1077, 1221
Bse8I GATNNNNATC 2 cut(s) 432, 457
BseDI CCNNGG 3 cut(s) 372, 774, 943
BseGI GGATG 6 cut(s) 214, 365, 458, 550, 1048, 1198
BseJI GATNNNNATC 2 cut(s) 432, 457
BseLI CCNNNNNNNGG 3 cut(s) 246, 449, 1182
BseMI GCAATG 2 cut(s) 1077, 1221
BseMII CTCAG 1 cut(s) 816
BseNI ACTGG 3 cut(s) 97, 715, 820
BseRI GAGGAG 1 cut(s) 1223
BseSI GKGCMC 1 cut(s) 442
BseXI GCAGC 5 cut(s) 822, 1003, 1294, 1310, 1322
Bsh1236I CGCG 2 cut(s) 645, 1228
BshFI GGCC 2 cut(s) 564, 743
BsiHKAI GWGCWC 2 cut(s) 442, 790
BsiHKCI CYCGRG 1 cut(s) 1143
BsiSI CCGG 1 cut(s) 1118
BslI CCNNNNNNNGG 3 cut(s) 246, 449, 1182
BsmI GAATGC 1 cut(s) 655
BsnI GGCC 2 cut(s) 564, 743
BsoBI CYCGRG 1 cut(s) 1143
Bsp1286I GDGCHC 2 cut(s) 442, 790
Bsp143I GATC 8 cut(s) 220, 347, 433, 838, 973, 1120, 1236, 1291
Bsp19I CCATGG 1 cut(s) 372
Bsp68I TCGCGA 1 cut(s) 645
BspACI CCGC 2 cut(s) 1228, 1234
BspANI GGCC 2 cut(s) 564, 743
BspCNI CTCAG 1 cut(s) 817
BspFNI CGCG 2 cut(s) 645, 1228
BspHI TCATGA 2 cut(s) 55, 322
BspLI GGNNCC 3 cut(s) 349, 1122, 1238
BspMAI CTGCAG 2 cut(s) 1300, 1312
BspPI GGATC 8 cut(s) 342, 355, 441, 1115, 1128, 1231, 1244, 1286
BsrDI GCAATG 2 cut(s) 1077, 1221
BsrI ACTGG 3 cut(s) 97, 715, 820
BssECI CCNNGG 3 cut(s) 372, 774, 943
BssMI GATC 8 cut(s) 220, 347, 433, 838, 973, 1120, 1236, 1291
BssNAI GTATAC 1 cut(s) 582
BssSI CACGAG 1 cut(s) 1050
BssT1I CCWWGG 1 cut(s) 372
Bst1107I GTATAC 1 cut(s) 582
Bst2BI CACGAG 1 cut(s) 1050
Bst4CI ACNGT 6 cut(s) 859, 917, 944, 1038, 1270, 1366
Bst6I CTCTTC 2 cut(s) 666, 943
BstC8I GCNNGC 2 cut(s) 1177, 1332
BstDEI CTNAG 1 cut(s) 825
BstDSI CCRYGG 2 cut(s) 372, 943
BstF5I GGATG 6 cut(s) 214, 365, 458, 550, 1048, 1198
BstFNI CGCG 2 cut(s) 645, 1228
BstHHI GCGC 1 cut(s) 1228
BstKTI GATC 8 cut(s) 223, 350, 436, 841, 976, 1123, 1239, 1294
BstMBI GATC 8 cut(s) 220, 347, 433, 838, 973, 1120, 1236, 1291
BstMWI GCNNNNNNNGC 8 cut(s) 405, 779, 827, 878, 1086, 1295, 1304, 1307
BstSFI CTRYAG 3 cut(s) 873, 1296, 1308
BstSLI GKGCMC 1 cut(s) 442
BstUI CGCG 2 cut(s) 645, 1228
BstV1I GCAGC 5 cut(s) 822, 1003, 1294, 1310, 1322
BstV2I GAAGAC 2 cut(s) 12, 481
BstX2I RGATCY 4 cut(s) 220, 347, 1120, 1236
BstXI CCANNNNNNTGG 1 cut(s) 596
BstYI RGATCY 4 cut(s) 220, 347, 1120, 1236
BstZ17I GTATAC 1 cut(s) 582
BsuI GTATCC 1 cut(s) 446
BsuRI GGCC 2 cut(s) 564, 743
BtgI CCRYGG 2 cut(s) 372, 943
BtgZI GCGATG 1 cut(s) 971
BtsCI GGATG 6 cut(s) 214, 365, 458, 550, 1048, 1198
BtsI GCAGTG 3 cut(s) 75, 349, 1320
BtsIMutI CAGTG 8 cut(s) 75, 90, 349, 708, 813, 1043, 1275, 1320
BtuMI TCGCGA 1 cut(s) 645
Cac8I GCNNGC 2 cut(s) 1177, 1332
CciI TCATGA 2 cut(s) 55, 322
CfoI GCGC 1 cut(s) 1228
Cfr13I GGNCC 1 cut(s) 562
Csp6I GTAC 3 cut(s) 290, 940, 1318
CviAII CATG 9 cut(s) 56, 153, 271, 303, 323, 373, 541, 614, 1132
CviQI GTAC 3 cut(s) 290, 940, 1318
DdeI CTNAG 1 cut(s) 825
DpnI GATC 8 cut(s) 222, 349, 435, 840, 975, 1122, 1238, 1293
DpnII GATC 8 cut(s) 220, 347, 433, 838, 973, 1120, 1236, 1291
Eam1104I CTCTTC 2 cut(s) 666, 943
EarI CTCTTC 2 cut(s) 666, 943
EciI GGCGGA 1 cut(s) 1249
Ecl136II GAGCTC 1 cut(s) 788
Eco130I CCWWGG 1 cut(s) 372
Eco147I AGGCCT 1 cut(s) 743
Eco24I GRGCYC 1 cut(s) 790
Eco53kI GAGCTC 1 cut(s) 788
Eco57I CTGAAG 2 cut(s) 119, 1323
Eco88I CYCGRG 1 cut(s) 1143
EcoICRI GAGCTC 1 cut(s) 788
EcoT14I CCWWGG 1 cut(s) 372
EcoT22I ATGCAT 1 cut(s) 367
EcoT38I GRGCYC 1 cut(s) 790
ErhI CCWWGG 1 cut(s) 372
FaeI CATG 9 cut(s) 59, 156, 274, 306, 326, 376, 544, 617, 1135
FalI AAGNNNNNCTT 2 cut(s) 728, 760
FatI CATG 9 cut(s) 55, 152, 270, 302, 322, 372, 540, 613, 1131
FbaI TGATCA 1 cut(s) 838
FblI GTMKAC 2 cut(s) 581, 1377
Fnu4HI GCNGC 5 cut(s) 811, 1017, 1299, 1308, 1311
FokI GGATG 6 cut(s) 221, 352, 445, 557, 1055, 1205
FriOI GRGCYC 1 cut(s) 790
Fsp4HI GCNGC 5 cut(s) 811, 1017, 1299, 1308, 1311
FspBI CTAG 2 cut(s) 528, 1172
GlaI GCGC 1 cut(s) 1227
GluI GCNGC 5 cut(s) 811, 1017, 1299, 1308, 1311
GsuI CTGGAG 1 cut(s) 1041
HaeIII GGCC 2 cut(s) 564, 743
HapII CCGG 1 cut(s) 1118
HhaI GCGC 1 cut(s) 1228
Hin1II CATG 9 cut(s) 59, 156, 274, 306, 326, 376, 544, 617, 1135
Hin6I GCGC 1 cut(s) 1226
HinP1I GCGC 1 cut(s) 1226
HindIII AAGCTT 1 cut(s) 1386
HinfI GANTC 8 cut(s) 52, 62, 158, 379, 523, 548, 716, 923
HpaII CCGG 1 cut(s) 1118
HphI GGTGA 8 cut(s) 245, 344, 388, 725, 739, 932, 1274, 1292
Hpy166II GTNNAC 5 cut(s) 149, 440, 582, 1318, 1378
Hpy188I TCNGA 6 cut(s) 51, 99, 225, 361, 553, 826
Hpy188III TCNNGA 8 cut(s) 56, 323, 644, 851, 927, 1124, 1143, 1145
Hpy8I GTNNAC 5 cut(s) 149, 440, 582, 1318, 1378
Hpy99I CGWCG 1 cut(s) 1184
HpyAV CCTTC 3 cut(s) 412, 730, 754
HpyCH4III ACNGT 6 cut(s) 859, 917, 944, 1038, 1270, 1366
HpyCH4IV ACGT 1 cut(s) 649
HpyF10VI GCNNNNNNNGC 8 cut(s) 405, 779, 827, 878, 1086, 1295, 1304, 1307
HpyF3I CTNAG 1 cut(s) 825
HpySE526I ACGT 1 cut(s) 649
Hsp92II CATG 9 cut(s) 59, 156, 274, 306, 326, 376, 544, 617, 1135
HspAI GCGC 1 cut(s) 1226
Ksp22I TGATCA 1 cut(s) 838
Kzo9I GATC 8 cut(s) 220, 347, 433, 838, 973, 1120, 1236, 1291
LmnI GCTCC 6 cut(s) 727, 785, 793, 853, 1085, 1286
Lsp1109I GCAGC 5 cut(s) 822, 1003, 1294, 1310, 1322
LweI GCATC 4 cut(s) 352, 374, 760, 948
MaeI CTAG 2 cut(s) 528, 1172
MaeII ACGT 1 cut(s) 649
MaeIII GTNAC 4 cut(s) 126, 185, 1074, 1217
MalI GATC 8 cut(s) 222, 349, 435, 840, 975, 1122, 1238, 1293
MboI GATC 8 cut(s) 220, 347, 433, 838, 973, 1120, 1236, 1291
MboII GAAGA 8 cut(s) 17, 400, 486, 500, 683, 960, 1004, 1166
MfeI CAATTG 1 cut(s) 751
MflI RGATCY 4 cut(s) 220, 347, 1120, 1236
MhlI GDGCHC 2 cut(s) 442, 790
MluCI AATT 4 cut(s) 244, 634, 681, 751
MlyI GAGTC 1 cut(s) 167
Mph1103I ATGCAT 1 cut(s) 367
MroXI GAANNNNTTC 1 cut(s) 422
MseI TTAA 4 cut(s) 243, 309, 1401, 1414
MslI CAYNNNNRTG 1 cut(s) 374
MspA1I CMGCKG 1 cut(s) 1301
MspI CCGG 1 cut(s) 1118
MunI CAATTG 1 cut(s) 751
Mva1269I GAATGC 1 cut(s) 655
MvnI CGCG 2 cut(s) 645, 1228
MwoI GCNNNNNNNGC 8 cut(s) 405, 779, 827, 878, 1086, 1295, 1304, 1307
NcoI CCATGG 1 cut(s) 372
NdeII GATC 8 cut(s) 220, 347, 433, 838, 973, 1120, 1236, 1291
NlaIII CATG 9 cut(s) 59, 156, 274, 306, 326, 376, 544, 617, 1135
NlaIV GGNNCC 3 cut(s) 349, 1122, 1238
NmeAIII GCCGAG 1 cut(s) 799
NmuCI GTSAC 2 cut(s) 1074, 1217
NruI TCGCGA 1 cut(s) 645
NsiI ATGCAT 1 cut(s) 367
OliI CACNNNNGTG 1 cut(s) 374
PaeR7I CTCGAG 1 cut(s) 1143
PagI TCATGA 2 cut(s) 55, 322
PceI AGGCCT 1 cut(s) 743
PctI GAATGC 1 cut(s) 655
PdmI GAANNNNTTC 1 cut(s) 422
PfeI GAWTC 7 cut(s) 52, 62, 379, 523, 548, 716, 923
PflMI CCANNNNNTGG 1 cut(s) 246
PkrI GCNGC 5 cut(s) 812, 1018, 1300, 1309, 1312
PleI GAGTC 1 cut(s) 166
PpsI GAGTC 1 cut(s) 166
PshBI ATTAAT 1 cut(s) 243
Psp124BI GAGCTC 1 cut(s) 790
PspN4I GGNNCC 3 cut(s) 349, 1122, 1238
PspPI GGNCC 1 cut(s) 562
PsrI GAACNNNNNNTAC 2 cut(s) 1047, 1079
PstI CTGCAG 2 cut(s) 1300, 1312
PsuI RGATCY 4 cut(s) 220, 347, 1120, 1236
PvuII CAGCTG 1 cut(s) 1301
RruI TCGCGA 1 cut(s) 645
RsaI GTAC 3 cut(s) 291, 941, 1319
RsaNI GTAC 3 cut(s) 290, 940, 1318
RseI CAYNNNNRTG 1 cut(s) 374
SacI GAGCTC 1 cut(s) 790
SaqAI TTAA 4 cut(s) 243, 309, 1401, 1414
SatI GCNGC 5 cut(s) 811, 1017, 1299, 1308, 1311
Sau3AI GATC 8 cut(s) 220, 347, 433, 838, 973, 1120, 1236, 1291
Sau96I GGNCC 1 cut(s) 562
SchI GAGTC 1 cut(s) 167
SduI GDGCHC 2 cut(s) 442, 790
SfaNI GCATC 4 cut(s) 352, 374, 760, 948
SfcI CTRYAG 3 cut(s) 873, 1296, 1308
Sfr274I CTCGAG 1 cut(s) 1143
SlaI CTCGAG 1 cut(s) 1143
SmiMI CAYNNNNRTG 1 cut(s) 374
SmlI CTYRAG 2 cut(s) 15, 1143
SmoI CTYRAG 2 cut(s) 15, 1143
Sse9I AATT 4 cut(s) 244, 634, 681, 751
SseBI AGGCCT 1 cut(s) 743
SsiI CCGC 2 cut(s) 1228, 1234
SspMI CTAG 2 cut(s) 528, 1172
SstI GAGCTC 1 cut(s) 790
StuI AGGCCT 1 cut(s) 743
StyI CCWWGG 1 cut(s) 372
TaaI ACNGT 6 cut(s) 859, 917, 944, 1038, 1270, 1366
TaiI ACGT 1 cut(s) 652
TaqI TCGA 2 cut(s) 426, 1144
TasI AATT 4 cut(s) 244, 634, 681, 751
TfiI GAWTC 7 cut(s) 52, 62, 379, 523, 548, 716, 923
Tru1I TTAA 4 cut(s) 243, 309, 1401, 1414
Tru9I TTAA 4 cut(s) 243, 309, 1401, 1414
TscAI CASTG 8 cut(s) 75, 97, 349, 715, 820, 1043, 1275, 1320
TseFI GTSAC 2 cut(s) 1074, 1217
TseI GCWGC 5 cut(s) 810, 1016, 1298, 1307, 1310
Tsp45I GTSAC 2 cut(s) 1074, 1217
TspDTI ATGAA 9 cut(s) 44, 225, 259, 291, 529, 561, 717, 776, 1017
TspRI CASTG 8 cut(s) 75, 97, 349, 715, 820, 1043, 1275, 1320
Van91I CCANNNNNTGG 1 cut(s) 246
VneI GTGCAC 1 cut(s) 438
VspI ATTAAT 1 cut(s) 243
XhoI CTCGAG 1 cut(s) 1143
XmiI GTMKAC 2 cut(s) 581, 1377
XmnI GAANNNNTTC 1 cut(s) 422
XspI CTAG 2 cut(s) 528, 1172
Zsp2I ATGCAT 1 cut(s) 367
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.