AT5G61140

Sec63 Brl domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0008552
Physical Location & Seq
Forward (+)
24589906 .. 24603522
13617 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G61140.1

Sequence Viewer

Length: 6441 bp
ATGGTCCACAACTCCCCATGTAATAAACATAAAACGACGTCGATTTCGTGGACCCGTATCTTAGCGGGTCGGTGTACGGGCCTATTTGTTCAAGTATCTAACCGACGCCGTTTTAGAACGCAAGCGATCGCCTTCTTCCTCGGAGAGCCGACCAGAGGTGAAAACACCACAGAGGAGAAGAATCCTAAACGACACAAGTACAGGGATTTTGTCATGTTAGTTCAGCTTCCTCGCTTGACAAGCTCTCTTAGAGAGCCTTTCGACATCGATCAGGCTTACCTTCGCCGCAAAACTATACTCCAAACTCTTAATAAGCCTCGCAGCTCCGGTAATCGCCTTGATGAATCTGACCTTGCTAAGAGGATAGTTCATCAATGGGAAGGAGCTTCTCTCGAAGTGCGACAAGCATACAAGCAGTTTATTGGAGCTGTAGTGGAATTGATTGATCGGGAAGTTCCATCAGATGAGTTTCGCGAGGTTGCTTTTTCCGCTTATCGTCTGTTTAACAATCCAGTGGAGGAAGATGATAGTGATATCAATGATAATATAAGCATTTCCGGGAAGAAGTTAGAGTTGCAAAATCTTGTCGGGCATGCTGTCTCAGATGCTAATGTAAAAAACGTGGCCTCCTTTGCTCAGGCACTTTACTCAATACAGCCTACTCATCAGTCTGAGACATATGCTGATGAAGTTAATGGGGGAGCTGAGTTTGGTGCCGACCTTGTTTTCAATCTACCAGCTCGTTTTTTGGTGGAAGCATCTCTTGACGAAACTGGGTTTGTGGATGTAGAAAGCAATGATGCTCATACATCGTTTTCTGAAGGATGGAGTGGTGTTAGCGACACGAAAAATAATCTCAGTGCAGGAAAATTCAATTTAAGTTGGCTAAGAGATGCATGTGGTCGGATGGTTAGAGAAACCAATTCACAACTTTCCCGTGAAGAATTGGCTATGGCTATATGCAGATTTCTGGATTCGGATAAACCCGGTGAGGAGATAGCTGGGGATCTGCTGGATCTTGTTGGGGATGGTGCATTTGAGACTGTTCAAGACCTTATTATGCATCGGAAAGAAATTGTTGATGCTATCCATCATGGCCAAATGATTCTCAAGTCTGACAAAGCAGCTTCAAACACCCAATCTCGGATGCCTACCTATGGAACACAGGTTACTGTTCAAACAGAGTCTGCAAAGCAAATTGAAAAACTTCGGCGTAAGGAGGAAAAGAAAAATAAACGAAATGCTGATCTTGGTTTAGAGAGTGAAATATCAGAGGCCAATTTTTCTTCTTTACTTGAGGCAAGTGAAAAGAAGACTGCATTTGAGGATCTGATTGGGTCTGGAGAAGCGAATTCTCTTGCACTTGCCCTTCCCCAAGGTACTGTAAGGAAGCACTTAAAAGGATATGAGGAAGTGTTTATCCCGCCAACCCCTACTGCACAAATGAAACCTGGCGAGAAGCTGATTGAGATAAAAGAGTTAGATGACTTTGCTCAAGCTGCTTTTCATGGTTACAAGTCTTTAAACCGTATTCAGAGTCGCATATTTCAGACAGTTTACCACACCAATGAGAATATATTGGTCTGTGCTCCAACAGGAGCTGGTAAAACAAATATAGCTATGATTTCTGTTCTACATGAGATCAAACAACACTTCAGGGATGGCTACTTGCACAAAAATGAGTTTAAGATTGTTTATGTAGCTCCTATGAAGGCGTTAGCAGCAGAGGTCACGTCAGCATTTAGCCGTCGTCTAGCTCCATTAAATATGGTTGTGAAGGAGCTTACAGGGGATATGCAACTCACGAAGACTGAACTTGAAGAAACTCAGATGATAGTGACAACACCAGAGAAATGGGATGTCATTACTCGCAAAAGCAGTGACATGTCAATGTCAATGCTAGTAAAACTATTGATCATTGATGAAGTGCATCTTCTGAATGATGATAGAGGTGCAGTGATAGAGGCGCTAGTTGCTCGGACCCTCCGTCAGGTGGAGTCAACTCAGACAATGATAAGAATTGTAGGCCTGTCAGCCACTTTACCTAGCTACCTACAGGTTGCTCAGTTTCTACGAGTGAATACAGACACTGGCCTATTCTATTTTGATTCAAGTTATCGCCCAGTTCCCTTAGCTCAACAATACATAGGCATCACTGAGCATAACTTTGCTGCTAGAAACGAATTATTAAATGAGATATGCTACAAAAAGGTTGTTGATTCAATTAAACAAGGTCATCAAGCAATGATATTTGTTCATTCCCGAAAAGACACTTCAAAAACAGCTGAGAAACTGGTTGATCTTGCCCGGCAATATGAAACTCTGGATTTGTTCACGAATGAGACTCATCCTCAATTTCAACTGATGAAGAAAGACGTTATGAAGTCCAGAAATAAGGATCTTGTCAAATTCTTTGAAGCTGGCTTCGGAATTCATCATGCTGGGATGTTGAGATCTGATAGAACACTTACGGAGAGGCTGTTTTCTGATGGTCTCTTGAAGGTGCTTGTTTGCACTGCAACTCTTGCGTGGGGTGTAAATCTGCCTGCACACACAGTCGTAATTAAGGGAACACAATTGTATGATGCCAAGGCTGGTGGGTGGAAAGACTTGGGAATGCTTGATGTCATGCAGATCTTTGGAAGGGCAGGGAGACCACAATTTGATAAGAGTGGTGAGGGAATTATCATCACATCCCATGACAAGCTGGCATACTATCTCCGGCTGCTGACTAGTCAGCTTCCCATAGAAAGTCAGTTCATAAGCTCCTTGAAAGATAACTTAAACGCAGAAGTGGTTTTGGGAACTGTAACTAATGTAAAGGAAGCCTGTGCTTGGCTTGGATATACATATTTGTCAATTCGGATGAAGTTAAATCCTTTGGCTTATGGCATTGGATGGGAAGAGATAATTGCCGATCCTTCTTTATCCTTGAAGCAACGAGCCCTTGTTGCTGATGCTGCCCGTTCACTGGACAAAGCTAAGATGATGAGGTTCGATGAGAAAAGTGGAAACTTTTACTGTACCGAGCTTGGACGGGTTGCAAGTCACTTCTATATTCAATATTCTAGTGTTGAAACATACAACGAAATGTTGAAGCGGCACATGAATGAAAGCGAGATAATTAATATGGTTGCCCATTCTTCTGAATTTGAAAATATTGTGGTAAGAGAGGAAGAGCAGCATGAACTTGAGACTCTTGCACGCTCTTGCTGTCCCTTAGAAGTTAAGGGAGGCCCTTCTAACAAACATGGAAAAATTTCGATTCTTATTCAGTTGTACATATCCCGTGGATCAATTGATGCATTTTCTCTGGTCTCTGATGCTTCATACATTAGTGCTAGTTTAGCACGTATAATGCGTGCTCTGTTTGAAATATGCTTGCGAAAAGGCTGGTGTGAGATGACTTTATTTATGTTGGAATACTGCAAGGCAGTTGATCGTCAACTATGGCCTCATCAGCATCCTCTTCGGCAATTCGAAAGGGATCTGCCTTCTGATCGAAGGGATGACTTAGACCATTTGTATGAGATGGAGGAAAAGGAAATTGGAGCGCTGATTCGTTATAATCCTGGGGGAAGGCATCTAGGATATTTTCCTTCAATTCAGTTGGCAGCTACTGTTAGTCCAATCACCCGCACTGTATTGAAGGTGGATTTGCTTATAACTCCAAATTTCATATGGAAGGATCGTTTTCATGGTACTGCTCTTCGTTGGTGGATTCTCATTGAGGACACAGAGAATGATTATATCTATCACTCAGATCTTTTCACACTAACGAAGCGGATGGCTAGAGGGGAACCACAGAAGCTTTCTTTTACCGTACCGATATTTGAACCACACCCACCACAATACTATGTTCATGCGGTTTCTGATTCTTGGTTGCATGCAGAGACTTACTTCACAATCTCTTTTCACAATCTTGCACTACCAGAGGCGCGGACGTCTCACACAGAGCTTTTAGACTTAAAACCTCTTCCTGTGACTTCTCTTGGGAATAAGTTGTATGAATCGCTTTACAAATTTTCACATTTCAATCCAATACAGACTCAGATTTTTCATGTGTTATATCACACAGACAACAATGTTCTTGTAGGAGCTCCAACTGGAAGTGGGAAAACTATATCTGCTGAACTTGCAATGTTGCGTCTCTTCAGTACCCAGCCTGATATGAAGGTTGTTTACATAGCACCACTGAAGGCTATTGTTCGAGAAAGGATGAATGATTGGAAAAAGCATCTTGTTGCTCCACTTGGAAAAGAAATGGTTGAAATGACTGGAGATTACACTCCAGATTTAGTAGCTCTCTTGTCCGCGGACATCATCATATCTACCCCTGAAAAATGGGATGGCATTAGTCGTAATTGGCATACTCGAAGTTATGTCAAAAAGGTCGGCCTTGTTATTTTGGATGAGATTCACTTGCTTGGAGCCGACAGAGGACCGATTCTTGAGGTTATTGTATCTAGGATGAGATATATATCATCACAGACAGAGCGCTCCGTCCGGTTTGTTGGGCTTTCTACAGCTCTGGCAAATGCAGGTGATTTGGCTGACTGGTTGGGTGTTGGGGAGATCGGACTTTTCAATTTTAAACCAAGCGTCAGGCCTGTACCAATTGAAGTTCATATTCAGGGTTATCCTGGAAAGTATTACTGCCCAAGGATGAATAGCATGAACAAGCCAGCATATGCAGCCATATGTACTCATTCACCTACGAAACCTGTTCTTATCTTTGTTTCATCCCGTAGGCAGACAAGGCTTACTGCATTGGATCTTATTCAGTTTGCTGCCTCCGACGAACATCCGCGGCAATTTCTGAGTGTGTCAGAGGAAGACTTGCAGATGGTCCTCTCACAGATAACTGATCAAAACCTTCGACATACGTTGCAATTCGGCATCGGACTGCATCATGCAGGTTTAAACGACCACGATAGATCTGCAGTCGAAGAGCTTTTCACGAACAACAAGATTCAGGTTTTGGTCTCAACAAGTACCTTGGCATGGGGAGTGAACCTACCAGCTCACTTAGTTATTATAAAGGGAACAGAATATTTCGACGGGAAAACCAAAAGATATGTTGACTTCCCTCTTACAGAGATCTTGCAAATGATGGGACGTGCAGGGCGACCACAGTTTGACCAACATGGGAAAGCGGTTATTCTTGTACATGAACCTAAAAAAAGCTTTTACAAAAAGTTCTTGTACGAACCATTCCCCGTAGAAAGTAGTTTGAAAGAGAAGCTGCACGATCACTTCAATGCGGAAATCGTCTCTGGAACAATAGGCAACAAAGAAGATGCAGTGCATTATCTTACATGGACGTATTTGTTTCGTAGACTGATGGCTAATCCTGCTTACTATGGGCTAGAAGGTACTCAAGACGAAACAATCTGTTCCTACTTATCAAGATTGGTGCAAACCACATTTGAGGATCTTGAAGACAGTGGTTGCCTCAAAGTAAATGAAGATAGTGTGGAGCCTACGATGCTGGGTACAATAGCGTCACAGTATTACCTTTGCTACATGACCGTGTCTATGTTTGGCTCTAATATAGGTCCTGACACATCGCTTGAAGCATTTTTGCATATCTTGGCTGGTGCTTCTGAGTATGATGAACTTCCTGTGCGGCACAATGAGGAAAATTACAATAAAACACTGTCAGATAGAGTTCGGTATCCCGTGGATAATAACCATCTAGATGATCCTCATGTGAAAGCGAATCTGCTCTTCCAGGCACATTTTTCTCAATTGGCACTTCCAATCAGTGATTATAACACGGATTTAAAGTCAGTACTGGATCAAAGCATTCGGATCCTCCAGGCCATGATTGATATCTGCGCAAACAGTGGATGGCTCTCAAGCTCACTGACGTGTATGCGCCTTTTGCAAATGGTCATGCAGGGCATGTGGTCTGATCAAGATTCGTCATTATGGATGATCCCATGCATGAATGACCTTCTTCTAGGATCTCTGACCGCAAGAGGGATTCACACGCTACATCAGCTATTAAATCTTCCAAGGGAAACTCTACAAAGTGTAACCGAAAACTTCCCTGCATCAAGATTATCTCAGGATCTCCAACGATTCCCACGGATCCAAATGAATGTGAGACTCCAAAAGAAGGATTCCGATGGCAAGAAAAAGCCTTCAACGCTGGAAATCAGATTGGAAAAGACATCAAAGAGGAATAGCTCGAGGGCATTGGCCCCTCGATTCCCAAAGGTGAAAGACGAAGCGTGGTGGTTGGTTCTTGGTGATACTTCAACTTCTGAGCTCTTTGCTGTGAAGAGAGTTTCGTTCACTGGTCGTCTGATCACACGCATGGAGTTACCTCCCAATATAACCTCTTTTCAGGATACGAAACTCATCTTAGTTTCGGATTGTTATTTGGGGTTTGAACAAGAACACTCCATTGAACAACTTGCACGACGCGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0005488 GO:0097159 GO:1901363
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DEAD Helicase_C Sec63

Protein Analysis

2146

Amino Acids

243.17

Weight (kDa)

6.39

Isoelectric Point (pI)

40.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DExH14_plug PF24557 116 - 220 5e-44 DExH14 plug domain
DEAD PF00270 509 - 683 5.7e-30 DEAD/DEAH box helicase
ResIII PF04851 514 - 682 3.2e-12 Type III restriction enzyme, res subunit
Helicase_C PF00271 798 - 886 4.3e-07 Helicase conserved C-terminal domain
WHD_SNRNP200 PF23445 917 - 988 1.4e-14 Winged Helix-turn-helix domain
Sec63 PF02889 1008 - 1304 2.6e-88 Sec63 Brl domain
DEAD PF00270 1346 - 1512 1.2e-27 DEAD/DEAH box helicase
ResIII PF04851 1358 - 1479 1.4e-10 Type III restriction enzyme, res subunit
Helicase_C PF00271 1624 - 1711 1.9e-09 Helicase conserved C-terminal domain
WHD_SNRNP200 PF23445 1742 - 1823 2.6e-40 Winged Helix-turn-helix domain
Sec63 PF02889 1828 - 2140 7.8e-69 Sec63 Brl domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 4 cut(s) 3567, 3665, 5039, 5775
AarI CACCTGC 1 cut(s) 4532
AatII GACGTC 2 cut(s) 41, 3947
Acc16I TGCGCA 1 cut(s) 5842
Acc36I ACCTGC 2 cut(s) 4532, 4907
AccB1I GGYRCC 1 cut(s) 713
AccI GTMKAC 1 cut(s) 5338
AccII CGCG 5 cut(s) 474, 3940, 4316, 4810, 6435
AcoI YGGCCR 1 cut(s) 1096
AcsI RAATTY 8 cut(s) 869, 1351, 2408, 2430, 3149, 3258, 3673, 4022
AcuI CTGAAG 4 cut(s) 840, 1639, 4138, 4217
AcyI GRCGYC 3 cut(s) 38, 106, 3944
AfeI AGCGCT 2 cut(s) 3555, 4499
AflIII ACRYGT 2 cut(s) 1884, 5873
AhdI GACNNNNNGTC 1 cut(s) 1986
AhlI ACTAGT 1 cut(s) 2732
AjiI CACGTC 3 cut(s) 1734, 5120, 5874
AjnI CCWGG 5 cut(s) 1450, 3571, 4642, 5733, 5820
AjuI GAANNNNNNNTTGG 2 cut(s) 3531, 3563
AleI CACNNNNGTG 1 cut(s) 5872
Alw21I GWGCWC 4 cut(s) 1591, 3367, 4102, 6279
AlwNI CAGNNNCTG 4 cut(s) 1187, 1601, 2090, 3620
Ama87I CYCGRG 1 cut(s) 6196
Aor51HI AGCGCT 2 cut(s) 3555, 4499
ApoI RAATTY 8 cut(s) 869, 1351, 2408, 2430, 3149, 3258, 3673, 4022
ArsI GACNNNNNNTTYG 2 cut(s) 28, 60
AseI ATTAAT 1 cut(s) 3126
AsiSI GCGATCGC 1 cut(s) 129
Asp700I GAANNNNTTC 2 cut(s) 1206, 3259
AspLEI GCGC 6 cut(s) 1969, 3556, 3940, 4500, 5843, 5883
AspS9I GGNCC 9 cut(s) 4, 51, 79, 1980, 3236, 4442, 4849, 5558, 6208
AsuC2I CCSGG 3 cut(s) 559, 987, 2308
AsuHPI GGTGA 8 cut(s) 170, 1001, 2687, 3625, 4556, 4704, 6238, 6269
AsuII TTCGAA 1 cut(s) 3480
AvaI CYCGRG 1 cut(s) 6196
AvaII GGWCC 6 cut(s) 4, 51, 1980, 4442, 4849, 5558
BalI TGGCCA 1 cut(s) 1098
BamHI GGATCC 2 cut(s) 5814, 6096
BanI GGYRCC 1 cut(s) 713
BanII GRGCYC 3 cut(s) 2947, 4102, 6279
BarI GAAGNNNNNNTAC 2 cut(s) 4166, 4198
BbsI GAAGAC 4 cut(s) 1319, 1814, 4842, 5448
Bbv12I GWGCWC 4 cut(s) 1591, 3367, 4102, 6279
BceAI ACGGC 2 cut(s) 93, 1731
BcgI CGANNNNNNTGC 4 cut(s) 3452, 3486, 4922, 4956
BciT130I CCWGG 5 cut(s) 1452, 3573, 4644, 5735, 5822
BciVI GTATCC 2 cut(s) 5688, 6352
BclI TGATCA 4 cut(s) 1914, 4867, 5917, 6315
BcnI CCSGG 3 cut(s) 559, 987, 2308
BcuI ACTAGT 1 cut(s) 2732
BfmI CTRYAG 4 cut(s) 429, 2054, 4524, 4941
BfoI RGCGCY 3 cut(s) 1970, 3557, 4501
BfuAI ACCTGC 2 cut(s) 4532, 4907
BfuI GTATCC 2 cut(s) 5688, 6352
BglII AGATCT 5 cut(s) 2453, 2634, 3763, 4937, 5100
BmcAI AGTACT 1 cut(s) 5796
Bme1390I CCNGG 8 cut(s) 559, 987, 1452, 2308, 3573, 4644, 5735, 5822
Bme18I GGWCC 6 cut(s) 4, 51, 1980, 4442, 4849, 5558
BmeRI GACNNNNNGTC 1 cut(s) 1986
BmeT110I CYCGRG 1 cut(s) 6196
BmgBI CACGTC 3 cut(s) 1734, 5120, 5874
BmgT120I GGNCC 9 cut(s) 4, 51, 79, 1980, 3236, 4442, 4849, 5558, 6208
BmiI GGNNCC 9 cut(s) 53, 715, 1982, 3801, 4432, 5481, 5816, 6098, 6210
BmrFI CCNGG 8 cut(s) 559, 987, 1452, 2308, 3573, 4644, 5735, 5822
BmrI ACTGGG 2 cut(s) 783, 2117
BmuI ACTGGG 2 cut(s) 783, 2117
BpiI GAAGAC 4 cut(s) 1319, 1814, 4842, 5448
BplI GAGNNNNNCTC 2 cut(s) 375, 407
BpmI CTGGAG 4 cut(s) 1362, 4275, 4299, 5804
Bpu10I CCTNAGC 2 cut(s) 636, 2131
Bpu14I TTCGAA 1 cut(s) 3480
BpuEI CTTGAG 7 cut(s) 1094, 1316, 1479, 3212, 4472, 5364, 5845
BpuMI CCSGG 3 cut(s) 559, 987, 2308
Bsa29I ATCGAT 1 cut(s) 267
BsaAI YACGTR 1 cut(s) 3353
BsaBI GATNNNNATC 2 cut(s) 4480, 5834
BsaHI GRCGYC 3 cut(s) 38, 106, 3944
BsaI GGTCTC 4 cut(s) 2498, 2647, 3322, 4990
BsaWI WCCGGW 2 cut(s) 326, 4506
BsaXI ACNNNNNCTCC 6 cut(s) 417, 447, 611, 641, 2465, 2495
Bse3DI GCAATG 3 cut(s) 802, 2250, 4146
Bse8I GATNNNNATC 2 cut(s) 4480, 5834
BseBI CCWGG 5 cut(s) 1452, 3573, 4644, 5735, 5822
BseCI ATCGAT 1 cut(s) 267
BseJI GATNNNNATC 2 cut(s) 4480, 5834
BseMI GCAATG 3 cut(s) 802, 2250, 4146
BseRI GAGGAG 2 cut(s) 188, 1007
BseYI CCCAGC 4 cut(s) 1001, 2441, 4161, 5491
BsgI GTGCAG 6 cut(s) 882, 1422, 1974, 2532, 5142, 5231
Bsh1236I CGCG 5 cut(s) 474, 3940, 4316, 4810, 6435
Bsh1285I CGRYCG 1 cut(s) 129
BshNI GGYRCC 1 cut(s) 713
BshVI ATCGAT 1 cut(s) 267
BsiEI CGRYCG 1 cut(s) 129
BsiHKAI GWGCWC 4 cut(s) 1591, 3367, 4102, 6279
BsiHKCI CYCGRG 1 cut(s) 6196
BsiSI CCGG 6 cut(s) 327, 558, 987, 2308, 2722, 4507
BslFI GGGAC 2 cut(s) 3201, 5130
BsmBI CGTCTC 3 cut(s) 3951, 4154, 5278
BsmFI GGGAC 2 cut(s) 3201, 5130
BsmI GAATGC 2 cut(s) 2622, 5808
Bso31I GGTCTC 4 cut(s) 2498, 2647, 3322, 4990
BsoBI CYCGRG 1 cut(s) 6196
Bsp119I TTCGAA 1 cut(s) 3480
Bsp1286I GDGCHC 5 cut(s) 1591, 2947, 3367, 4102, 6279
Bsp1407I TGTACA 2 cut(s) 3279, 5167
Bsp68I TCGCGA 1 cut(s) 474
BspDI ATCGAT 1 cut(s) 267
BspFNI CGCG 5 cut(s) 474, 3940, 4316, 4810, 6435
BspLI GGNNCC 9 cut(s) 53, 715, 1982, 3801, 4432, 5481, 5816, 6098, 6210
BspMAI CTGCAG 1 cut(s) 4945
BspMI ACCTGC 2 cut(s) 4532, 4907
BspQI GCTCTTC 4 cut(s) 3171, 3714, 4944, 5735
BspT104I TTCGAA 1 cut(s) 3480
BspT107I GGYRCC 1 cut(s) 713
BspTNI GGTCTC 4 cut(s) 2498, 2647, 3322, 4990
BsrDI GCAATG 3 cut(s) 802, 2250, 4146
BsrGI TGTACA 2 cut(s) 3279, 5167
BssNI GRCGYC 3 cut(s) 38, 106, 3944
BssT1I CCWWGG 5 cut(s) 1375, 2589, 4661, 4998, 6020
Bst2UI CCWGG 5 cut(s) 1452, 3573, 4644, 5735, 5822
Bst6I CTCTTC 9 cut(s) 2898, 3171, 3474, 3714, 3981, 4157, 4944, 5735, 6284
BstACI GRCGYC 3 cut(s) 38, 106, 3944
BstAPI GCANNNNNTGC 1 cut(s) 2525
BstAUI TGTACA 2 cut(s) 3279, 5167
BstBAI YACGTR 1 cut(s) 3353
BstBI TTCGAA 1 cut(s) 3480
BstDSI CCRYGG 5 cut(s) 3289, 4314, 4808, 5682, 6092
BstENI CCTNNNNNAGG 1 cut(s) 1988
BstFNI CGCG 5 cut(s) 474, 3940, 4316, 4810, 6435
BstH2I RGCGCY 3 cut(s) 1970, 3557, 4501
BstHHI GCGC 6 cut(s) 1969, 3556, 3940, 4500, 5843, 5883
BstMCI CGRYCG 1 cut(s) 129
BstNI CCWGG 5 cut(s) 1452, 3573, 4644, 5735, 5822
BstNSI RCATGY 5 cut(s) 596, 900, 1888, 3890, 5911
BstSCI CCNGG 8 cut(s) 557, 985, 1450, 2306, 3571, 4642, 5733, 5820
BstSFI CTRYAG 4 cut(s) 429, 2054, 4524, 4941
BstUI CGCG 5 cut(s) 474, 3940, 4316, 4810, 6435
BstV2I GAAGAC 4 cut(s) 1319, 1814, 4842, 5448
BstXI CCANNNNNNTGG 1 cut(s) 1854
Bsu15I ATCGAT 1 cut(s) 267
BsuI GTATCC 2 cut(s) 5688, 6352
BsuTUI ATCGAT 1 cut(s) 267
BtgI CCRYGG 5 cut(s) 3289, 4314, 4808, 5682, 6092
BtgZI GCGATG 1 cut(s) 5553
BtrI CACGTC 3 cut(s) 1734, 5120, 5874
BtsI GCAGTG 4 cut(s) 1885, 1962, 2514, 5310
BtuMI TCGCGA 1 cut(s) 474
BveI ACCTGC 2 cut(s) 4532, 4907
CaiI CAGNNNCTG 4 cut(s) 1187, 1601, 2090, 3620
CfoI GCGC 6 cut(s) 1969, 3556, 3940, 4500, 5843, 5883
Cfr13I GGNCC 9 cut(s) 4, 51, 79, 1980, 3236, 4442, 4849, 5558, 6208
Cfr42I CCGCGG 2 cut(s) 4317, 4811
ClaI ATCGAT 1 cut(s) 267
CseI GACGC 4 cut(s) 114, 4136, 4591, 5493
CspCI CAANNNNNGTGG 2 cut(s) 2578, 2613
DraI TTTAAA 4 cut(s) 1524, 4594, 4923, 5787
DriI GACNNNNNGTC 1 cut(s) 1986
EaeI YGGCCR 1 cut(s) 1096
Eam1104I CTCTTC 9 cut(s) 2898, 3171, 3474, 3714, 3981, 4157, 4944, 5735, 6284
Eam1105I GACNNNNNGTC 1 cut(s) 1986
EarI CTCTTC 9 cut(s) 2898, 3171, 3474, 3714, 3981, 4157, 4944, 5735, 6284
Ecl136II GAGCTC 2 cut(s) 4100, 6277
Eco130I CCWWGG 5 cut(s) 1375, 2589, 4661, 4998, 6020
Eco147I AGGCCT 2 cut(s) 2028, 4609
Eco24I GRGCYC 3 cut(s) 2947, 4102, 6279
Eco31I GGTCTC 4 cut(s) 2498, 2647, 3322, 4990
Eco32I GATATC 2 cut(s) 535, 5836
Eco47I GGWCC 6 cut(s) 4, 51, 1980, 4442, 4849, 5558
Eco47III AGCGCT 2 cut(s) 3555, 4499
Eco53kI GAGCTC 2 cut(s) 4100, 6277
Eco57I CTGAAG 4 cut(s) 840, 1639, 4138, 4217
Eco88I CYCGRG 1 cut(s) 6196
EcoICRI GAGCTC 2 cut(s) 4100, 6277
EcoNI CCTNNNNNAGG 1 cut(s) 1988
EcoO109I RGGNCCY 2 cut(s) 3236, 5558
EcoRI GAATTC 2 cut(s) 1351, 2430
EcoRII CCWGG 5 cut(s) 1450, 3571, 4642, 5733, 5820
EcoRV GATATC 2 cut(s) 535, 5836
EcoT14I CCWWGG 5 cut(s) 1375, 2589, 4661, 4998, 6020
EcoT22I ATGCAT 4 cut(s) 898, 1065, 3307, 5951
EcoT38I GRGCYC 3 cut(s) 2947, 4102, 6279
ErhI CCWWGG 5 cut(s) 1375, 2589, 4661, 4998, 6020
Esp3I CGTCTC 3 cut(s) 3951, 4154, 5278
FalI AAGNNNNNCTT 4 cut(s) 747, 779, 1917, 1949
FaqI GGGAC 2 cut(s) 3201, 5130
FauI CCCGC 3 cut(s) 58, 1431, 3644
FauNDI CATATG 4 cut(s) 679, 3680, 4690, 4700
FbaI TGATCA 4 cut(s) 1914, 4867, 5917, 6315
FblI GTMKAC 1 cut(s) 5338
FriOI GRGCYC 3 cut(s) 2947, 4102, 6279
FspI TGCGCA 1 cut(s) 5842
GlaI GCGC 6 cut(s) 1968, 3555, 3939, 4499, 5842, 5882
GsaI CCCAGC 4 cut(s) 1005, 2445, 4165, 5495
GsuI CTGGAG 4 cut(s) 1362, 4275, 4299, 5804
HaeII RGCGCY 3 cut(s) 1970, 3557, 4501
HapII CCGG 6 cut(s) 327, 558, 987, 2308, 2722, 4507
HgaI GACGC 4 cut(s) 114, 4136, 4591, 5493
HhaI GCGC 6 cut(s) 1969, 3556, 3940, 4500, 5843, 5883
Hin1I GRCGYC 3 cut(s) 38, 106, 3944
Hin6I GCGC 6 cut(s) 1967, 3554, 3938, 4498, 5841, 5881
HinP1I GCGC 6 cut(s) 1967, 3554, 3938, 4498, 5841, 5881
HincII GTYRAC 3 cut(s) 2001, 3446, 5083
HindII GTYRAC 3 cut(s) 2001, 3446, 5083
HindIII AAGCTT 2 cut(s) 3809, 5185
HpaII CCGG 6 cut(s) 327, 558, 987, 2308, 2722, 4507
HphI GGTGA 8 cut(s) 170, 1001, 2687, 3625, 4556, 4704, 6238, 6269
Hpy99I CGWCG 7 cut(s) 40, 43, 108, 1752, 4802, 5063, 6435
Hsp92I GRCGYC 3 cut(s) 38, 106, 3944
HspAI GCGC 6 cut(s) 1967, 3554, 3938, 4498, 5841, 5881
Ksp22I TGATCA 4 cut(s) 1914, 4867, 5917, 6315
KspI CCGCGG 2 cut(s) 4317, 4811
LguI GCTCTTC 4 cut(s) 3171, 3714, 4944, 5735
MfeI CAATTG 4 cut(s) 2576, 3297, 4617, 5750
MhlI GDGCHC 5 cut(s) 1591, 2947, 3367, 4102, 6279
MlsI TGGCCA 1 cut(s) 1098
MluNI TGGCCA 1 cut(s) 1098
MlyI GAGTC 7 cut(s) 1193, 1546, 2006, 2338, 3190, 4042, 6108
MmeI TCCRAC 6 cut(s) 884, 1616, 3399, 4127, 4821, 6106
Mox20I TGGCCA 1 cut(s) 1098
Mph1103I ATGCAT 4 cut(s) 898, 1065, 3307, 5951
MroXI GAANNNNTTC 2 cut(s) 1206, 3259
MscI TGGCCA 1 cut(s) 1098
Msp20I TGGCCA 1 cut(s) 1098
MspA1I CMGCKG 3 cut(s) 2285, 4316, 4810
MspI CCGG 6 cut(s) 327, 558, 987, 2308, 2722, 4507
MspR9I CCNGG 8 cut(s) 559, 987, 1452, 2308, 3573, 4644, 5735, 5822
MssI GTTTAAAC 1 cut(s) 4923
MunI CAATTG 4 cut(s) 2576, 3297, 4617, 5750
Mva1269I GAATGC 2 cut(s) 2622, 5808
MvaI CCWGG 5 cut(s) 1452, 3573, 4644, 5735, 5822
MvnI CGCG 5 cut(s) 474, 3940, 4316, 4810, 6435
NciI CCSGG 3 cut(s) 559, 987, 2308
NdeI CATATG 4 cut(s) 679, 3680, 4690, 4700
NlaIV GGNNCC 9 cut(s) 53, 715, 1982, 3801, 4432, 5481, 5816, 6098, 6210
NmuCI GTSAC 6 cut(s) 1729, 1837, 1880, 3047, 3982, 5505
NruI TCGCGA 1 cut(s) 474
NsbI TGCGCA 1 cut(s) 5842
NsiI ATGCAT 4 cut(s) 898, 1065, 3307, 5951
NspI RCATGY 5 cut(s) 596, 900, 1888, 3890, 5911
NspV TTCGAA 1 cut(s) 3480
OliI CACNNNNGTG 1 cut(s) 5872
PaeI GCATGC 2 cut(s) 596, 3890
PaeR7I CTCGAG 1 cut(s) 6196
PaqCI CACCTGC 1 cut(s) 4532
PceI AGGCCT 2 cut(s) 2028, 4609
PciI ACATGT 1 cut(s) 1884
PciSI GCTCTTC 4 cut(s) 3171, 3714, 4944, 5735
PcsI WCGNNNNNNNCGW 4 cut(s) 44, 1984, 3358, 5125
PctI GAATGC 2 cut(s) 2622, 5808
PdmI GAANNNNTTC 2 cut(s) 1206, 3259
PflFI GACNNNGTC 1 cut(s) 5533
PfoI TCCNGGA 2 cut(s) 557, 4642
Ple19I CGATCG 1 cut(s) 129
PleI GAGTC 7 cut(s) 1192, 1545, 2005, 2338, 3190, 4042, 6108
PmeI GTTTAAAC 1 cut(s) 4923
PpsI GAGTC 7 cut(s) 1192, 1545, 2005, 2338, 3190, 4042, 6108
Ppu21I YACGTR 1 cut(s) 3353
PpuMI RGGWCCY 1 cut(s) 5558
PscI ACATGT 1 cut(s) 1884
PshBI ATTAAT 1 cut(s) 3126
PsiI TTATAA 4 cut(s) 3567, 3665, 5039, 5775
Psp124BI GAGCTC 2 cut(s) 4102, 6279
Psp5II RGGWCCY 1 cut(s) 5558
Psp6I CCWGG 5 cut(s) 1450, 3571, 4642, 5733, 5820
PspFI CCCAGC 4 cut(s) 1001, 2441, 4161, 5491
PspGI CCWGG 5 cut(s) 1450, 3571, 4642, 5733, 5820
PspN4I GGNNCC 9 cut(s) 53, 715, 1982, 3801, 4432, 5481, 5816, 6098, 6210
PspPI GGNCC 9 cut(s) 4, 51, 79, 1980, 3236, 4442, 4849, 5558, 6208
PspPPI RGGWCCY 1 cut(s) 5558
PspXI VCTCGAGB 1 cut(s) 6196
PsrI GAACNNNNNNTAC 2 cut(s) 1153, 1185
PstI CTGCAG 1 cut(s) 4945
PstNI CAGNNNCTG 4 cut(s) 1187, 1601, 2090, 3620
PsyI GACNNNGTC 1 cut(s) 5533
PvuI CGATCG 1 cut(s) 129
PvuII CAGCTG 1 cut(s) 2285
RgaI GCGATCGC 1 cut(s) 129
RruI TCGCGA 1 cut(s) 474
SacI GAGCTC 2 cut(s) 4102, 6279
SacII CCGCGG 2 cut(s) 4317, 4811
SapI GCTCTTC 4 cut(s) 3171, 3714, 4944, 5735
Sau96I GGNCC 9 cut(s) 4, 51, 79, 1980, 3236, 4442, 4849, 5558, 6208
ScaI AGTACT 1 cut(s) 5796
SchI GAGTC 7 cut(s) 1193, 1546, 2006, 2338, 3190, 4042, 6108
ScrFI CCNGG 8 cut(s) 559, 987, 1452, 2308, 3573, 4644, 5735, 5822
SduI GDGCHC 5 cut(s) 1591, 2947, 3367, 4102, 6279
SfaAI GCGATCGC 1 cut(s) 129
SfcI CTRYAG 4 cut(s) 429, 2054, 4524, 4941
Sfr274I CTCGAG 1 cut(s) 6196
Sfr303I CCGCGG 2 cut(s) 4317, 4811
SfuI TTCGAA 1 cut(s) 3480
SgfI GCGATCGC 1 cut(s) 129
SgrBI CCGCGG 2 cut(s) 4317, 4811
SinI GGWCC 6 cut(s) 4, 51, 1980, 4442, 4849, 5558
SlaI CTCGAG 1 cut(s) 6196
SmlI CTYRAG 8 cut(s) 1109, 1295, 1494, 3191, 4451, 5379, 5860, 6196
SmoI CTYRAG 8 cut(s) 1109, 1295, 1494, 3191, 4451, 5379, 5860, 6196
SpeI ACTAGT 1 cut(s) 2732
SphI GCATGC 2 cut(s) 596, 3890
SseBI AGGCCT 2 cut(s) 2028, 4609
SspI AATATT 3 cut(s) 3065, 3160, 5054
SstI GAGCTC 2 cut(s) 4102, 6279
StuI AGGCCT 2 cut(s) 2028, 4609
StyD4I CCNGG 8 cut(s) 557, 985, 1450, 2306, 3571, 4642, 5733, 5820
StyI CCWWGG 5 cut(s) 1375, 2589, 4661, 4998, 6020
TaqII GACCGA 1 cut(s) 4459
TatI WGTACW 5 cut(s) 198, 3279, 4703, 5167, 5794
TauI GCSGC 4 cut(s) 288, 3103, 4813, 5632
TseFI GTSAC 6 cut(s) 1729, 1837, 1880, 3047, 3982, 5505
Tsp45I GTSAC 6 cut(s) 1729, 1837, 1880, 3047, 3982, 5505
TspGWI ACGGA 5 cut(s) 1976, 2486, 4492, 5795, 6109
Tth111I GACNNNGTC 1 cut(s) 5533
VpaK11BI GGWCC 6 cut(s) 4, 51, 1980, 4442, 4849, 5558
VspI ATTAAT 1 cut(s) 3126
XagI CCTNNNNNAGG 1 cut(s) 1988
XapI RAATTY 8 cut(s) 869, 1351, 2408, 2430, 3149, 3258, 3673, 4022
XbaI TCTAGA 1 cut(s) 5698
XceI RCATGY 5 cut(s) 596, 900, 1888, 3890, 5911
XcmI CCANNNNNNNNNTGG 1 cut(s) 3678
XhoI CTCGAG 1 cut(s) 6196
XmiI GTMKAC 1 cut(s) 5338
XmnI GAANNNNTTC 2 cut(s) 1206, 3259
ZraI GACGTC 2 cut(s) 39, 3945
ZrmI AGTACT 1 cut(s) 5796
Zsp2I ATGCAT 4 cut(s) 898, 1065, 3307, 5951
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.