FvH4_5g13990

Activating signal cointegrator 1 complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0008552
Physical Location & Seq
Forward (+)
7907946 .. 7927539
19594 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g13990.t11

Sequence Viewer

Length: 6246 bp
ATGTTGGTTCAGCTTCCGCGATTGACAAGCTCTCTGAGGGAGCCCTTCGACGTCGACCAGGCCTATCTGCAGCGCAAGCTCATCCTTCAAAACCAGACCAAGCCTCGCCAATCATCGAGCTCAGTCGATGAGTCGGAGCTTGCGCGAAAGATAGTTCATAGATGGGAGGAAGCTTCGTATGAGCTGCGGCAAGCTTACAAGCAGTTTATTGGAGCTGTGGTGGAGCTGATAGATGGAGAAGTTCAATCCGAAGAGTTTCGCGAGGTGGCACTCGCCGTGTACCGGCTTTTTGGCAGGCCAGAGGAGGAAGGCAGTGTTGAAACTAATTTTTCTGGAAAAAAGCAGGAAGTGCAAATGCTTTTGGGTCACACAGTCTCAGATGCCAACATGCGGAAAGTTGCGTCTCTAGCTCAGGAACTTTCTGGGATGCAGTCTAGTGATCATGGGATTACACTTGTTTCAGAAACGCCCGTGAATGGAACTCATGACAGTGCAGAATTTGGTGCCGACCTGGTTTTTCATCCACCAGCTCGGTTTTTTGTAGATGTTTCTTTGGATGATGGGGAGTCATTTTGTGAGGAAACAGCTGGACCCTCCTCTTACTATGAAGGATCATATGGTGATGGTGGCTTGATCGGTCTTCATTCTGCTACTGATGGGCGCGGCATCAATCTGAGTTGGCTACAAGATGCATGTGATCAGATAACAAAAAGTTCTACACAGCTTTCTCGAGATGAACTGGCGATGGCCATATGTCGGGTGCTTGATTCTGACAAAGCTGGTGACGAGATAGCAGGTGACTTGTTGGATCTTGTGGGGGATAGTGCGTTTGAAACCGTTCAAGACCTCATATCACATCGAAAGGAACTTGTTGATGCGATCCATCATGGGTTACTAGTAATGAAATCTGACAAGTCTTCTTTGACCTCACAACCTCGCATGCCTAGTTATGGCACACAGGTGACTGTTCAAACAGAATCTGAACGGCAGATTGATAAGCTTCGGCGCAAAGAGGAAAAGCGAAATAGACGAGGCACAGAATATGGCACTGATAATGATCTGGCTGGTGTTAATTTTTCTTCTCTACTACAAGCAAGTGAAAGAAAAAATCTTTTTGATGATCTATCGGGGCTCGGTGAAGGACTAGCTGTTAATGCTCTTCCTCAAGGAACTCAGAGGAAGCACCATAAGGGGTATGAGGAGGTTCTAATCCCTCCAACACCAGGAGCACAAATGAAACCTGGAGAAAAGCTGATTGATATAGCGGAGTTAGATGACTTTGCTCAAGCTGCTTTTCGTGGATACAAATCTTTGAACCGCATTCAGAGCCGCATATATCATACTGTTTATTATACAAATGAGAATATACTAGTTTGTGCTCCAACAGGAGCTGGCAAGACAAACATAGCTATGATTTCCATTCTACACGAGATTGGACAGCACTTCAAAGATGGTTTCTTGCACAAAGATGAGTTTAAGATAGTTTATGTTGCACCTATGAAGGCGTTAGCAGCAGAAGTCACATCAACTTTTAGCCAACGATTATCTCCATTGAATATGACTGTGAGAGAACTTACTGGAGATATGCAACTTTCTAAGAATGAGCTTGAAGAAACTCAGATGATAGTTACAACACCTGAGAAATGGGATGTAATTACACGCAAGAGCAGTGACATGTCACTTTCAATGCTGGTGAAGCTCCTAATAATTGATGAAGTTCATCTATTGAATGATGATAGAGGCCCTGTTATTGAGGCACTAGTTGCTCGGACTTTACGCCAGGTGGAGTCTTCACAGTCAATGATACGCATTGTTGGACTATCCGCCACTCTACCCAACTATTTGGAGGTTGCACAGTTTCTGAGAGTGAATCCAGAGGCAGGACTCTTCTACTTTGATTCTAGCTACCGTCCAGTGCCCCTTGCACAGCAGTACATTGGCATTACTGAGACAAACTATCCAGCCAAACTTGAATTGTTGAATGAGATATGCTACAAGAAGGTTGTTGAGTCACTAAGGCAAGGTCATCAGGCAATGGTGTTTGTCCATTCTCGAAAGGACACGGCAAAAACAGCTCAGAAGCTGGTTGAACTTGCTCGCAAATTTGAAGGACTGGAGCTCTTTAAGAATGATCAGCACCCACTGTTTTCATTGAAACAGAGGGATGTTGTCAAATCCAGAAACAAAGATCTTGTTGAACTTTTTGAATTCGGACTTGGGATTCATAATGCTGGGATGTTACGTTCTGATAGAGTTCTGACTGAGCGCCTTTTCTCTGATGGACTATTGAAGGTACTTGTTTGTACAGCAACTTTGGCATGGGGAGTAAATCTACCAGCTCACACTGTGGTGATAAAGGGGACCCAATTATATGATCCAAAGGCTGGTGGGTGGCGAGACCTTGGAATGCTTGATGTTATGCAGATATTTGGACGGGCTGGGAGACCTCAATTCGATAAAAGCGGTGAAGGCATTATAATCACGTCTCATGACAAACTGGCCTACTATCTGCGGTTACTGACGAGTCAACTTCCTATTGAAAGTCAGTTCATTAGTTCCTTGAAGGACAATTTAAATGCTGAGGTGGCACTTGGTACTGTCACCAATGTCAAGGAAGCTTGTGCATGGCTTGGATATACATATCTTTTTATAAGAATGAGGCTTAACCCTTTGGCGTATGGTATCGCGTGGGAGGAGGTAATGGCAGATCCTTCCTTGAGTTTAAAGCAGAGATCACTCATAGCAGATGCTGCGCGGGATCTTGACAAAGCAAAAATGATGCGGTTTGACGAGAAAAGCGGAAATTTTTATTGTACAGAGCTTGGTCGCATAGCAAGCCACTTTTATATTCAATATTCTAGTGTTGAAACTTACAATGAAATGTTGAAGCGTCACATGAATGAGACTGAGGTAATTGACATGGTAGCCCACTCCTCGGAATTTCAGAATATTGTTGTTCGAGAAGAGGAACAAAATGAGCTTGAGATGTTGGTGCGGAAATTATGTCCACTAGAAGTTAAGGGTGGTCCTTCAAATAAACATGGAAAGATTTCCATTCTCATTCAGGTGTACATATCTAGAGGCTCAATTGATACCTTTTCTTTGGTTTCTGATGCTCAATATATAAGTGCAAGCTTGGCACGTATTATGCGGGCCTTATTTGAGATTTGTTTGCGAAAAGGCTGGAGTGAGATGACATTGTTCATGTTGGAATATTGCAAAGCAGTGGACCGCCAAGTTTGGCCCCATCAACATCCTTTCAGACAATTTGACAGGGATATCTCACCACAAATAATCCGAAATCTTGAGGAACGAGGGGCTGATCTTGATCGCCTATATGACATGGAGGAAAAAGAGATTGGAAAACTGGTCAATTATGGGCCTGGAGGAAGGAAGGTTAAGCAACATTTGGGATACTTTCCATGGATCCAGTTAGCTGCAACCGTAAGTCCAATTACCAGAACTGTTTTAAAGGTGGACTTGCTTATTACGCCAGACTTCATATGGAAAGATCAATTTCACGGCACTGCCCAGCGTTGGTGGATTCTAGTTGAGGATTCTGAGAATGATCATATCTACCACTCAGAGCTGTTCACTCTGACAAAGCGGATGGCTAAAGGGGAACCTCAGAAGCTCTCGTTCACTGTACCAATATTTGAGCCACATCCACCACAGTATTATATTCGTGCTGTTTCTGATTCTTGGTTGCAAGCAGAGGCATTCTACACCATTTCTTTCCATAATTTAGCACTCCCAGAGGCCCATACTTCACATACAGAACTGCTAGATCTGAAGCCTCTCCCTGTGACATCTCTTGGAAACAGTACTTATGAAGCATTGTACAAATTCTCACATTTTAATCCGATACAAACACAGACTTTCCATGTGCTGTATCACACAGACAATAATGTATTACTGGGAGCTCCAACTGGAAGTGGAAAAACTATTTCTGCCGAGCTTGCTATGCTTCATCTCTTCAACACCCAGCCTGATATGAAGGTCATTTACATAGCACCCTTGAAAGCTATTGTTCGAGAAAGAATGAATGATTGGAGGAAGCGCCTTGTCTCTCAGCTTGGGAAGAAAATGGTTGAAATGACAGGAGATTATACTCCAGATTTGATGGCCATCTTGTCAGCAGATATCATAATATCAACTCCTGAGAAATGGGATGGTATCAGTCGTAATTGGCATAGCCGAACCTATGTTAAAAAGGTTGGGCTTATGATTTTGGATGAGATTCACTTGTTGGGAGCTGACCGTGGGCCTATCCTCGAGGTTATTGTGTCAAGGATGCGATACATATCCTCACAGACAGAGCGTGAAGTACGGTTTGTAGGGCTTTCTACGGCTCTGGCAAATGCAGGAGATTTGGCTGATTGGTTGGGTGTCGGAGAAACTGGACTCTTCAATTTTAAACCAAGTGTGAGACCTGTACCCCTTGAAGTTCATATCCAGGGATATCCAGGAAAGTTCTATTGTCCAAGGATGAATAGCATGAACAAACCATCGTATGCTGCTATATGCACCCATTCACCAACAAAACCGGTTCTAATTTTTGTTTCATCACGTCGCCAGACAAGACTTACTGCATTGGACATCATTCAGTATGCAGCTTCGGATGAACATCCAAGGCAGTTTCTTAGTATACCAGAAGAAGAGCTCCAGATGGTTCTTTATCAGGTTGCTGATAGTAACCTGAGGCACACCTTGCAATTTGGCATTGGATTACACCATGCTGGTCTGAATGACAAAGATAGATCCCTGGTTGAGGAGCTTTTTGCAAACAACAAGATTCAGGTATTGGTTTGTACTAGCACATTGGCATGGGGTGTAAACCTTCCAGCGCATCTGGTTATTATAAAGGGAACAGAATTTTTTGATGGGAAAACAAAGAGATATGTTGACTTCCCAATCACAGATATCTTGCAAATGATGGGTCGTGCAGGTCGCCCACAATTTGATCAACACGGGAAAGCTGTCATTCTTGTTCATGAACCCAAAAAGAGCTTCTATAAAAAGTTTTTATATGAACCATTTCCCGTCGAGAGTAGTCTGAGGGAACAGCTGCATAATCACATCAATGCAGAGATAGTTTCTGGTACAATTTGCCATAAAGAGGATGCATTGCATTATCTGACATGGACATATTTATTCCGCAGACTGATGTTTAATCCCGCTTACTATGGCTTGGAAAATACTGACGCCGAAGTTTTAAGTTCTTATTTGTCTAGCTTAGTGCAGAACACATTGGAGGATCTGGAAGACAGTGGATGCATCAAGATGAGTGAGGACAGTGTGGAGCCAATGATGCTGGGTTCGATAGCATCCCAGTATTACCTTAGCTACTTGACCGTATCAATGTTTGGTTCAAACATTGGCTCTGATACCTCGCTTGAAGTATTCTTGCATATCCTATCTGCTGCTTCTGAATATGATGAACTTCCTGTTCGCCATAACGAGGAAAATTACAATGCGGTGCTGTCAGAGAGAGTTCGATGTAAAGTGGATAAGGATCGACTAGATGATCCACATGTCAAAGCAAATCTGCTGTTTCAGGCACATTTCTCTCAGCTAGAATTACCAATTAGTGACTATGTCACAGACTTGAAGTCAGTTTTGGATCAAAGTATACGGATTATCCAGGCTATGATAGATATATGTGCCAACAGCGGTTGGCTTTCAAGTTCAGTTACATGCATGCATCTTCTGCAGATGGTCATGCAGGGCTTATGGTTTGATGAGGATTCTTCTTTATGGATGTTGCCCTGCATGAATGCTGAACTTGCTGACTCACTCTGCAAACGAGGAATATTCCGTGTACAGCAGCTATTGGAACTTCCCAAGGCAACTTTGCAGAACATGATTGGAAACTTCCCTGCTTCAAAATTCTTTCAGGATCTGCAGCTGTTTCCACGAATCGAAGTAAAGTTGAAAATTTTATGGAAAGAGGGTGGAGAATCTTGCTCTTTAAACATCAGATTGATGAAGACCAATTTCAGAAAACATAAATCAAGAGCCTTTACTCCACGCTTCCCTAAGGTGAAAAATGAAGCATGGTGGTTGGTGCTTGGGAACACCGCAACGTCGGAACTATATGCTCTGAAGAGAGTATCTTTCTCTGATCACTTGGTTACTAACATGGAGTTACCATCAGATTCAACCACTCTCCAGGGGATGAAACTGATGGTAGTATCAGATTCTTATCTGGGGTTCGAACAAGAGCATTCAATATCAGAACTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DEAD Helicase_C Sec63

Protein Analysis

2082

Amino Acids

235.75

Weight (kDa)

5.92

Isoelectric Point (pI)

40.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DExH14_plug PF24557 45 - 144 1.8e-43 DExH14 plug domain
DEAD PF00270 439 - 613 4.9e-31 DEAD/DEAH box helicase
ResIII PF04851 450 - 612 9.9e-13 Type III restriction enzyme, res subunit
Helicase_C PF00271 733 - 816 3.6e-06 Helicase conserved C-terminal domain
WHD_SNRNP200 PF23445 847 - 926 2.8e-15 Winged Helix-turn-helix domain
Sec63 PF02889 938 - 1245 2.7e-88 Sec63 Brl domain
DEAD PF00270 1287 - 1361 2.8e-13 DEAD/DEAH box helicase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 2477, 2651, 4862
AarI CACCTGC 1 cut(s) 785
AatII GACGTC 1 cut(s) 54
AbsI CCTCGAGG 1 cut(s) 4274
Acc36I ACCTGC 2 cut(s) 785, 4937
AccB1I GGYRCC 1 cut(s) 503
AccB7I CCANNNNNTGG 1 cut(s) 2384
AccI GTMKAC 3 cut(s) 54, 4648, 5632
AccII CGCG 6 cut(s) 19, 145, 261, 663, 2687, 2755
AcoI YGGCCR 2 cut(s) 747, 4125
AcsI RAATTY 9 cut(s) 497, 2102, 2207, 2803, 2939, 3845, 4874, 5888, 5937
AcuI CTGAAG 2 cut(s) 3812, 6125
AcyI GRCGYC 2 cut(s) 51, 5205
AdeI CACNNNGTG 1 cut(s) 2347
AflIII ACRYGT 2 cut(s) 1674, 5533
AgeI ACCGGT 1 cut(s) 4546
AhdI GACNNNNNGTC 1 cut(s) 5611
AhlI ACTAGT 3 cut(s) 895, 1369, 1759
AjiI CACGTC 2 cut(s) 2484, 4571
AleI CACNNNNGTG 2 cut(s) 959, 2348
AloI GAACNNNNNNTCC 2 cut(s) 2227, 2259
Alw21I GWGCWC 6 cut(s) 122, 1231, 1381, 2121, 3925, 4665
Alw26I GTCTC 9 cut(s) 379, 408, 1943, 2391, 2437, 2490, 2897, 4072, 4423
AlwNI CAGNNNCTG 6 cut(s) 980, 1391, 1861, 2083, 2750, 5902
Ama87I CYCGRG 2 cut(s) 729, 4274
ApoI RAATTY 9 cut(s) 497, 2102, 2207, 2803, 2939, 3845, 4874, 5888, 5937
ArsI GACNNNNNNTTYG 2 cut(s) 4924, 4956
AsiGI ACCGGT 1 cut(s) 4546
Asp700I GAANNNNTTC 4 cut(s) 255, 837, 3049, 5037
AspLEI GCGC 8 cut(s) 75, 145, 663, 1008, 2268, 2755, 4062, 4849
AsuII TTCGAA 1 cut(s) 6216
AvaI CYCGRG 2 cut(s) 729, 4274
AvaII GGWCC 4 cut(s) 590, 2361, 3026, 3229
AxyI CCTNAGG 2 cut(s) 4700, 6039
BaeGI GKGCMC 1 cut(s) 1920
BalI TGGCCA 2 cut(s) 749, 4127
BamHI GGATCC 1 cut(s) 3426
BanI GGYRCC 1 cut(s) 503
BanII GRGCYC 6 cut(s) 45, 122, 1134, 2121, 3925, 4665
BarI GAAGNNNNNNTAC 2 cut(s) 3989, 4021
BauI CACGAG 1 cut(s) 1427
BbsI GAAGAC 5 cut(s) 632, 909, 1782, 5271, 5996
Bbv12I GWGCWC 6 cut(s) 122, 1231, 1381, 2121, 3925, 4665
BbvCI CCTCAGC 1 cut(s) 2580
BceAI ACGGC 5 cut(s) 260, 1001, 2079, 3538, 4365
BciVI GTATCC 2 cut(s) 1295, 3407
BclI TGATCA 6 cut(s) 439, 697, 2131, 3568, 4963, 6124
BcoDI GTCTC 9 cut(s) 379, 408, 1943, 2391, 2437, 2490, 2897, 4072, 4423
BcuI ACTAGT 3 cut(s) 895, 1369, 1759
BfmI CTRYAG 3 cut(s) 68, 5711, 5903
BfoI RGCGCY 2 cut(s) 2269, 4063
BfuAI ACCTGC 2 cut(s) 785, 4937
BfuI GTATCC 2 cut(s) 1295, 3407
BglII AGATCT 2 cut(s) 2188, 3787
BmcAI AGTACT 1 cut(s) 3826
Bme18I GGWCC 4 cut(s) 590, 2361, 3026, 3229
BmeRI GACNNNNNGTC 1 cut(s) 5611
BmeT110I CYCGRG 2 cut(s) 729, 4274
BmgBI CACGTC 2 cut(s) 2484, 4571
BmiI GGNNCC 9 cut(s) 42, 505, 592, 2362, 2363, 3245, 3428, 3624, 5304
BmrI ACTGGG 2 cut(s) 3926, 5326
BmuI ACTGGG 2 cut(s) 3926, 5326
BpiI GAAGAC 5 cut(s) 632, 909, 1782, 5271, 5996
BpmI CTGGAG 8 cut(s) 1263, 1599, 2135, 3205, 3405, 4098, 4649, 6155
Bpu10I CCTNAGC 3 cut(s) 411, 2580, 5342
Bpu14I TTCGAA 1 cut(s) 6216
BpuEI CTTGAG 5 cut(s) 1149, 1269, 2737, 3002, 3326
BsaAI YACGTR 1 cut(s) 3143
BsaBI GATNNNNATC 8 cut(s) 1056, 1306, 3327, 4127, 4303, 4626, 5514, 6204
BsaHI GRCGYC 2 cut(s) 51, 5205
BsaI GGTCTC 3 cut(s) 2391, 2437, 4423
BsaWI WCCGGW 1 cut(s) 4546
BsaXI ACNNNNNCTCC 2 cut(s) 204, 234
Bse118I RCCGGY 2 cut(s) 282, 4546
Bse21I CCTNAGG 2 cut(s) 4700, 6039
Bse3DI GCAATG 2 cut(s) 2040, 5126
Bse8I GATNNNNATC 8 cut(s) 1056, 1306, 3327, 4127, 4303, 4626, 5514, 6204
BseJI GATNNNNATC 8 cut(s) 1056, 1306, 3327, 4127, 4303, 4626, 5514, 6204
BseMI GCAATG 2 cut(s) 2040, 5126
BseRI GAGGAG 6 cut(s) 317, 586, 1214, 2708, 2923, 4787
BseSI GKGCMC 1 cut(s) 1920
BseYI CCCAGC 5 cut(s) 2231, 2438, 3531, 3984, 5314
BsgI GTGCAG 3 cut(s) 513, 4965, 5261
Bsh1236I CGCG 6 cut(s) 19, 145, 261, 663, 2687, 2755
BshNI GGYRCC 1 cut(s) 503
BshTI ACCGGT 1 cut(s) 4546
BsiHKAI GWGCWC 6 cut(s) 122, 1231, 1381, 2121, 3925, 4665
BsiHKCI CYCGRG 2 cut(s) 729, 4274
BsiSI CCGG 2 cut(s) 283, 4547
BslFI GGGAC 1 cut(s) 2374
BsmAI GTCTC 9 cut(s) 379, 408, 1943, 2391, 2437, 2490, 2897, 4072, 4423
BsmBI CGTCTC 2 cut(s) 408, 2490
BsmFI GGGAC 1 cut(s) 2374
BsmI GAATGC 5 cut(s) 1320, 2412, 3719, 5782, 6226
Bso31I GGTCTC 3 cut(s) 2391, 2437, 4423
BsoBI CYCGRG 2 cut(s) 729, 4274
Bsp119I TTCGAA 1 cut(s) 6216
Bsp1286I GDGCHC 9 cut(s) 45, 122, 1134, 1231, 1381, 1920, 2121, 3925, 4665
Bsp1407I TGTACA 5 cut(s) 2303, 2813, 3069, 3840, 5821
Bsp19I CCATGG 1 cut(s) 3422
Bsp68I TCGCGA 1 cut(s) 261
BspFNI CGCG 6 cut(s) 19, 145, 261, 663, 2687, 2755
BspHI TCATGA 3 cut(s) 484, 2488, 4993
BspLI GGNNCC 9 cut(s) 42, 505, 592, 2362, 2363, 3245, 3428, 3624, 5304
BspMAI CTGCAG 3 cut(s) 72, 5715, 5907
BspMI ACCTGC 2 cut(s) 785, 4937
BspQI GCTCTTC 2 cut(s) 1164, 4653
BspT104I TTCGAA 1 cut(s) 6216
BspT107I GGYRCC 1 cut(s) 503
BspTNI GGTCTC 3 cut(s) 2391, 2437, 4423
BsrDI GCAATG 2 cut(s) 2040, 5126
BsrFI RCCGGY 2 cut(s) 282, 4546
BsrGI TGTACA 5 cut(s) 2303, 2813, 3069, 3840, 5821
BssAI RCCGGY 2 cut(s) 282, 4546
BssNAI GTATAC 2 cut(s) 4649, 5633
BssNI GRCGYC 2 cut(s) 51, 5205
BssSI CACGAG 1 cut(s) 1427
BssT1I CCWWGG 5 cut(s) 2401, 3422, 4484, 4631, 5844
Bst1107I GTATAC 2 cut(s) 4649, 5633
Bst2BI CACGAG 1 cut(s) 1427
Bst6I CTCTTC 8 cut(s) 246, 1164, 1892, 2958, 3980, 4412, 4653, 6101
BstACI GRCGYC 2 cut(s) 51, 5205
BstAPI GCANNNNNTGC 5 cut(s) 349, 1763, 2750, 4711, 5710
BstAUI TGTACA 5 cut(s) 2303, 2813, 3069, 3840, 5821
BstBAI YACGTR 1 cut(s) 3143
BstBI TTCGAA 1 cut(s) 6216
BstDSI CCRYGG 2 cut(s) 3422, 4261
BstENI CCTNNNNNAGG 1 cut(s) 4769
BstFNI CGCG 6 cut(s) 19, 145, 261, 663, 2687, 2755
BstH2I RGCGCY 2 cut(s) 2269, 4063
BstHHI GCGC 8 cut(s) 75, 145, 663, 1008, 2268, 2755, 4062, 4849
BstMAI GTCTC 9 cut(s) 379, 408, 1943, 2391, 2437, 2490, 2897, 4072, 4423
BstNSI RCATGY 7 cut(s) 391, 696, 943, 1678, 5537, 5700, 5704
BstSFI CTRYAG 3 cut(s) 68, 5711, 5903
BstSLI GKGCMC 1 cut(s) 1920
BstUI CGCG 6 cut(s) 19, 145, 261, 663, 2687, 2755
BstV2I GAAGAC 5 cut(s) 632, 909, 1782, 5271, 5996
BstX2I RGATCY 9 cut(s) 808, 2188, 2707, 2758, 3426, 3787, 4760, 5257, 5899
BstXI CCANNNNNNTGG 1 cut(s) 1843
BstYI RGATCY 9 cut(s) 808, 2188, 2707, 2758, 3426, 3787, 4760, 5257, 5899
BstZ17I GTATAC 2 cut(s) 4649, 5633
Bsu36I CCTNAGG 2 cut(s) 4700, 6039
BsuI GTATCC 2 cut(s) 1295, 3407
BtgI CCRYGG 2 cut(s) 3422, 4261
BtgZI GCGATG 1 cut(s) 758
BtrI CACGTC 2 cut(s) 2484, 4571
BtsI GCAGTG 4 cut(s) 319, 1675, 3231, 3525
BtuMI TCGCGA 1 cut(s) 261
BveI ACCTGC 2 cut(s) 785, 4937
CaiI CAGNNNCTG 6 cut(s) 980, 1391, 1861, 2083, 2750, 5902
CciI TCATGA 3 cut(s) 484, 2488, 4993
CfoI GCGC 8 cut(s) 75, 145, 663, 1008, 2268, 2755, 4062, 4849
Cfr10I RCCGGY 2 cut(s) 282, 4546
CseI GACGC 3 cut(s) 390, 2879, 5213
CsiI ACCWGGT 1 cut(s) 510
CspAI ACCGGT 1 cut(s) 4546
DraI TTTAAA 5 cut(s) 2574, 2724, 3471, 4417, 5973
DraIII CACNNNGTG 1 cut(s) 2347
DriI GACNNNNNGTC 1 cut(s) 5611
EaeI YGGCCR 2 cut(s) 747, 4125
Eam1104I CTCTTC 8 cut(s) 246, 1164, 1892, 2958, 3980, 4412, 4653, 6101
Eam1105I GACNNNNNGTC 1 cut(s) 5611
EarI CTCTTC 8 cut(s) 246, 1164, 1892, 2958, 3980, 4412, 4653, 6101
EciI GGCGGA 1 cut(s) 1813
Ecl136II GAGCTC 4 cut(s) 120, 2119, 3923, 4663
Eco130I CCWWGG 5 cut(s) 2401, 3422, 4484, 4631, 5844
Eco147I AGGCCT 1 cut(s) 62
Eco24I GRGCYC 6 cut(s) 45, 122, 1134, 2121, 3925, 4665
Eco31I GGTCTC 3 cut(s) 2391, 2437, 4423
Eco32I GATATC 4 cut(s) 3280, 4144, 4463, 4924
Eco47I GGWCC 4 cut(s) 590, 2361, 3026, 3229
Eco53kI GAGCTC 4 cut(s) 120, 2119, 3923, 4663
Eco57I CTGAAG 2 cut(s) 3812, 6125
Eco81I CCTNAGG 2 cut(s) 4700, 6039
Eco88I CYCGRG 2 cut(s) 729, 4274
EcoICRI GAGCTC 4 cut(s) 120, 2119, 3923, 4663
EcoNI CCTNNNNNAGG 1 cut(s) 4769
EcoO109I RGGNCCY 2 cut(s) 1742, 2361
EcoRI GAATTC 1 cut(s) 2207
EcoRV GATATC 4 cut(s) 3280, 4144, 4463, 4924
EcoT14I CCWWGG 5 cut(s) 2401, 3422, 4484, 4631, 5844
EcoT22I ATGCAT 5 cut(s) 694, 5128, 5279, 5702, 5706
EcoT38I GRGCYC 6 cut(s) 45, 122, 1134, 2121, 3925, 4665
ErhI CCWWGG 5 cut(s) 2401, 3422, 4484, 4631, 5844
Esp3I CGTCTC 2 cut(s) 408, 2490
FalI AAGNNNNNCTT 2 cut(s) 3464, 3496
FaqI GGGAC 1 cut(s) 2374
FauI CCCGC 3 cut(s) 2748, 3144, 5185
FauNDI CATATG 3 cut(s) 616, 752, 3503
FbaI TGATCA 6 cut(s) 439, 697, 2131, 3568, 4963, 6124
FblI GTMKAC 3 cut(s) 54, 4648, 5632
FriOI GRGCYC 6 cut(s) 45, 122, 1134, 2121, 3925, 4665
GlaI GCGC 8 cut(s) 74, 144, 662, 1007, 2267, 2754, 4061, 4848
GsaI CCCAGC 5 cut(s) 2235, 2442, 3535, 3988, 5318
GsuI CTGGAG 8 cut(s) 1263, 1599, 2135, 3205, 3405, 4098, 4649, 6155
HaeII RGCGCY 2 cut(s) 2269, 4063
HapII CCGG 2 cut(s) 283, 4547
HgaI GACGC 3 cut(s) 390, 2879, 5213
HhaI GCGC 8 cut(s) 75, 145, 663, 1008, 2268, 2755, 4062, 4849
Hin1I GRCGYC 2 cut(s) 51, 5205
Hin6I GCGC 8 cut(s) 73, 143, 661, 1006, 2266, 2753, 4060, 4847
HinP1I GCGC 8 cut(s) 73, 143, 661, 1006, 2266, 2753, 4060, 4847
HincII GTYRAC 3 cut(s) 55, 2528, 4906
HindII GTYRAC 3 cut(s) 55, 2528, 4906
HindIII AAGCTT 5 cut(s) 171, 192, 998, 2616, 3133
HpaII CCGG 2 cut(s) 283, 4547
Hpy99I CGWCG 5 cut(s) 53, 56, 4575, 5048, 6091
HpyCH4IV ACGT 6 cut(s) 51, 2242, 2483, 3142, 4570, 6086
HpySE526I ACGT 6 cut(s) 51, 2242, 2483, 3142, 4570, 6086
Hsp92I GRCGYC 2 cut(s) 51, 5205
HspAI GCGC 8 cut(s) 73, 143, 661, 1006, 2266, 2753, 4060, 4847
KflI GGGWCCC 1 cut(s) 2361
Ksp22I TGATCA 6 cut(s) 439, 697, 2131, 3568, 4963, 6124
LguI GCTCTTC 2 cut(s) 1164, 4653
MabI ACCWGGT 1 cut(s) 510
MaeII ACGT 6 cut(s) 51, 2242, 2483, 3142, 4570, 6086
MfeI CAATTG 1 cut(s) 3087
MflI RGATCY 9 cut(s) 808, 2188, 2707, 2758, 3426, 3787, 4760, 5257, 5899
MhlI GDGCHC 9 cut(s) 45, 122, 1134, 1231, 1381, 1920, 2121, 3925, 4665
MlsI TGGCCA 2 cut(s) 749, 4127
MluNI TGGCCA 2 cut(s) 749, 4127
MlyI GAGTC 8 cut(s) 140, 575, 1796, 1878, 2018, 2533, 4398, 5786
MmeI TCCRAC 9 cut(s) 114, 786, 1241, 1406, 1795, 3189, 3950, 4372, 6069
Mox20I TGGCCA 2 cut(s) 749, 4127
Mph1103I ATGCAT 5 cut(s) 694, 5128, 5279, 5702, 5706
MroXI GAANNNNTTC 4 cut(s) 255, 837, 3049, 5037
MscI TGGCCA 2 cut(s) 749, 4127
Msp20I TGGCCA 2 cut(s) 749, 4127
MspA1I CMGCKG 4 cut(s) 587, 5068, 5673, 5908
MspI CCGG 2 cut(s) 283, 4547
MunI CAATTG 1 cut(s) 3087
Mva1269I GAATGC 5 cut(s) 1320, 2412, 3719, 5782, 6226
MvnI CGCG 6 cut(s) 19, 145, 261, 663, 2687, 2755
NcoI CCATGG 1 cut(s) 3422
NdeI CATATG 3 cut(s) 616, 752, 3503
NlaIV GGNNCC 9 cut(s) 42, 505, 592, 2362, 2363, 3245, 3428, 3624, 5304
NmeAIII GCCGAG 1 cut(s) 3979
NruI TCGCGA 1 cut(s) 261
NsiI ATGCAT 5 cut(s) 694, 5128, 5279, 5702, 5706
NspI RCATGY 7 cut(s) 391, 696, 943, 1678, 5537, 5700, 5704
NspV TTCGAA 1 cut(s) 6216
OliI CACNNNNGTG 2 cut(s) 959, 2348
PaeI GCATGC 2 cut(s) 943, 5704
PaeR7I CTCGAG 2 cut(s) 729, 4274
PagI TCATGA 3 cut(s) 484, 2488, 4993
PaqCI CACCTGC 1 cut(s) 785
PceI AGGCCT 1 cut(s) 62
PciI ACATGT 2 cut(s) 1674, 5533
PciSI GCTCTTC 2 cut(s) 1164, 4653
PctI GAATGC 5 cut(s) 1320, 2412, 3719, 5782, 6226
PdmI GAANNNNTTC 4 cut(s) 255, 837, 3049, 5037
PflFI GACNNNGTC 1 cut(s) 5597
PflMI CCANNNNNTGG 1 cut(s) 2384
PfoI TCCNGGA 1 cut(s) 4465
PinAI ACCGGT 1 cut(s) 4546
PleI GAGTC 8 cut(s) 139, 574, 1795, 1878, 2017, 2532, 4398, 5786
PpsI GAGTC 8 cut(s) 139, 574, 1795, 1878, 2017, 2532, 4398, 5786
Ppu21I YACGTR 1 cut(s) 3143
PpuMI RGGWCCY 1 cut(s) 2361
PscI ACATGT 2 cut(s) 1674, 5533
PsiI TTATAA 3 cut(s) 2477, 2651, 4862
Psp124BI GAGCTC 4 cut(s) 122, 2121, 3925, 4665
Psp5II RGGWCCY 1 cut(s) 2361
PspFI CCCAGC 5 cut(s) 2231, 2438, 3531, 3984, 5314
PspN4I GGNNCC 9 cut(s) 42, 505, 592, 2362, 2363, 3245, 3428, 3624, 5304
PspPPI RGGWCCY 1 cut(s) 2361
PspXI VCTCGAGB 1 cut(s) 4274
PstI CTGCAG 3 cut(s) 72, 5715, 5907
PstNI CAGNNNCTG 6 cut(s) 980, 1391, 1861, 2083, 2750, 5902
PsuI RGATCY 9 cut(s) 808, 2188, 2707, 2758, 3426, 3787, 4760, 5257, 5899
PsyI GACNNNGTC 1 cut(s) 5597
PvuII CAGCTG 3 cut(s) 587, 5068, 5908
RruI TCGCGA 1 cut(s) 261
SacI GAGCTC 4 cut(s) 122, 2121, 3925, 4665
SalI GTCGAC 1 cut(s) 53
SapI GCTCTTC 2 cut(s) 1164, 4653
ScaI AGTACT 1 cut(s) 3826
SchI GAGTC 8 cut(s) 140, 575, 1796, 1878, 2018, 2533, 4398, 5786
SduI GDGCHC 9 cut(s) 45, 122, 1134, 1231, 1381, 1920, 2121, 3925, 4665
SexAI ACCWGGT 1 cut(s) 510
SfcI CTRYAG 3 cut(s) 68, 5711, 5903
Sfr274I CTCGAG 2 cut(s) 729, 4274
SfuI TTCGAA 1 cut(s) 6216
SinI GGWCC 4 cut(s) 590, 2361, 3026, 3229
SlaI CTCGAG 2 cut(s) 729, 4274
SmiI ATTTAAAT 1 cut(s) 2574
SmlI CTYRAG 7 cut(s) 729, 1164, 1284, 2716, 2981, 3305, 4274
SmoI CTYRAG 7 cut(s) 729, 1164, 1284, 2716, 2981, 3305, 4274
SpeI ACTAGT 3 cut(s) 895, 1369, 1759
SphI GCATGC 2 cut(s) 943, 5704
SseBI AGGCCT 1 cut(s) 62
SspI AATATT 5 cut(s) 2855, 2950, 3215, 3654, 5814
SstI GAGCTC 4 cut(s) 122, 2121, 3925, 4665
StuI AGGCCT 1 cut(s) 62
StyI CCWWGG 5 cut(s) 2401, 3422, 4484, 4631, 5844
SwaI ATTTAAAT 1 cut(s) 2574
TaiI ACGT 6 cut(s) 54, 2245, 2486, 3145, 4573, 6089
TaqII GACCGA 1 cut(s) 626
TatI WGTACW 8 cut(s) 1932, 2303, 2813, 3069, 3824, 3840, 4811, 5821
TauI GCSGC 3 cut(s) 190, 666, 1332
TspGWI ACGGA 2 cut(s) 5650, 5807
Tth111I GACNNNGTC 1 cut(s) 5597
Van91I CCANNNNNTGG 1 cut(s) 2384
VpaK11BI GGWCC 4 cut(s) 590, 2361, 3026, 3229
XagI CCTNNNNNAGG 1 cut(s) 4769
XapI RAATTY 9 cut(s) 497, 2102, 2207, 2803, 2939, 3845, 4874, 5888, 5937
XbaI TCTAGA 1 cut(s) 3077
XceI RCATGY 7 cut(s) 391, 696, 943, 1678, 5537, 5700, 5704
XcmI CCANNNNNNNNNTGG 1 cut(s) 3501
XhoI CTCGAG 2 cut(s) 729, 4274
XmiI GTMKAC 3 cut(s) 54, 4648, 5632
XmnI GAANNNNTTC 4 cut(s) 255, 837, 3049, 5037
ZraI GACGTC 1 cut(s) 52
ZrmI AGTACT 1 cut(s) 3826
Zsp2I ATGCAT 5 cut(s) 694, 5128, 5279, 5702, 5706
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.