Rroxscaffold_3G00270920

Activating signal cointegrator 1 complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0008552
Physical Location & Seq
Forward (+)
63352405 .. 63372000
19596 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00270920.1

Sequence Viewer

Length: 6252 bp
ATGCTGGTTCAGCTTCCGCGATTGACTAGCTCTCTGAGGGAGCCCTTCGACGTCGACCAGGCCTATTTGCAGCGCAAGCTCATCCTTCAAAACCACACCAAGCCTCGCCAATCATCGAGCTCAGTTGATGAGTCGGAGCTTGCGAGAAAGATAGTTCATAGATGGGAGGAAGCAGCTTCGTATGAGCTGCGGCAAGCTTACAAGCAGTTTATTGGAGCTGTGGTGGAGTTGATAGATGGAGAAGTTCAATCCGAGGAGTTTCGTGAGGTGGCACTCGTTGTTTACCGGCTTTTTGGCAGACCGGAGGAGGAAGGCAGTGTTGAAACAAATTTTGCCGGAAAAAAGCAGGAAGTGCAAATGCTTTTGGGGCACGCAGTTTCAGATGTTAACATGCGGAAAGTTGCGTCTCTAGCTCAGAGACTTTGTGGGATGCAGTCTAGTGATCATGGGATTACACTTGTTTCAGAAAGGCCTGTAAATGGATCTCATGACGATGCAGAATTTGGTGCCGACCTTGTTTTTCATCCTCCGGCTCGGTTTTTGGTAGATGTTTCCTTGGATGATGCGGAGTCATTTTGTGAGGAAACCGCTGGACCTTCCTCTCTTTATGAAGGATCATATGGTCACAGTGGCTTGAGTGGCGGTCATTCTGCTACTGATCGGCGCAGCATCGATCTGACTTGGCTACAAGATGCATGTGATCAGATAACAAAAAGTACTACACAGCTTTCTCAAGATGAACTGGCTATGGCCATTTGTCGGGTGCTTGATTCTGACAAACCTGGTGACGAGATAGCAGGTGACTTGTTGGACCTTGTGGGGGATAGTGCATTTGAAACTGTTCAGGACCTCATATCACATCGAAAGGAACTTGTTGATGCAATCCATCATGGGTTACTAGTAATGAAATCTGACAAATCGTCTTTGACCTCTCAACCTCGCATGCCTAGTTATGGCACACAGGTTACTGTTCAAACAGAATCTGAACGGCAGATTGATAAGCTTCGGCGCAAAGAGGAAAAGCGAAATAGACGAGGAACAGAATATGGCACTGATAGTGATCTGGCTGCTGTAAATTTTTCTTCTCTACTACAAGCAAGTGAGAAAAAAAATATTTTTGATGATCTATCAGGCCTTGGTGAAGGGATAGCGGTTAATGCTCTTCCGCAAGGAACTCAGAGGAAGCACCACAAGGGGTATGAGGAGGTCCTAATTCCTCCAACACCAGGAGCACAAATGAAACCTGGAGAAAAGCTGATTGAGATCTCGGAGTTAGATGAGTTTGCTCAAGCTGCTTTTCGTGGATACAAATCTTTGAACCGCATTCAGAGCCGCATATATCATACTGTGTACTACACAAATGAGAATATACTAGTTTGTGCTCCAACAGGAGCTGGCAAAACAAACATAGCTATGATTTCCATTCTACATGAGATTGGACAGCACTTCAAGGATGGTTACCTGCACAAAGACGAGTTCAAAATAGTTTATGTTGCACCTATGAAGGCATTAGCTGCAGAAGTCACATCCACTTTTAGCCATCGGTTATCTCCATTGAATATGACTGTGAGAGAACTTACTGGAGACATGCAACTTTCTAAGAATGAACTTGAAGAAACTCAGATGATAGTTACAACACCTGAGAAATGGGATGTCATTACTCGCAAGAGCAGTGACATGTCACTTTCAATGCTGGTGAAACTCCTAATAATTGATGAAGTTCATCTTTTGAATGATGATAGAGGCCCGGTAATTGAGGCACTAGTTGCTCGGACTTTACGCCAGGTGGAGTCTTCACAGTCAATGATACGCATTGTGGGACTTTCCGCCACTCTACCCAACTATTTGGAGGTTGCACAGTTTCTGAGAGTGAATCCAGAGGCAGGCCTCTTCTACTTTGATTCTAGCTATCGTCCAGTGCCCCTTGCACAACAATATATTGGTATTAGTGAGACAAACTTTTCAGCCAAAACTGAATTGTTGAATGAGATATGCTACAAGAAGGTTGTGGAGTCACTAAGGCAAGGTCATCAGGCAATGGTATTTGTCCATTCTCGAAAGGACACAGCAAAAACGGCTCAGAAGCTGAGGGAAGTTGTAAAATCCAGAAACAAAGATCTTGTTGAACTTTTTGAATTCGGAATTGGAATTCATAATGCTGGGATGTTACGTTCTGATAGAGGTCTGACTGAGCGCCTTTTCTCTGATGGGCTATTGAAGGTACTTGTTTGTACAGCAACTTTGGCGTGGGGCGTAAATCTACCAGCTCACACTGTTGTGATAAAGGGGACCCAATTATATGATCCAAAGGCTGGTGGGTGGCGAGACCTGGGAATGCTTGATGTTATGCAGATATTTGGACGGGCTGGGAGACCTCAATTCGATAAAAGTGGTGAAGGTATTATAATCACGTCTCATGACAAGCTGGCCTACTATCTGCGGTTGCTGACAAGTCAACTTCCTATTGAAAGTCAGATTCATGTTCTGGTTCTACCTGTGGCTCTTATAAAATATGAGTATAGTGGTGATATTGATAATATTCATGTGTTCTCTAATGGAAAGCTATTCTTGTTTGCTCTAAATTCTGAATTCTGCTTGTTTTTCATTAGTTCCTTGAAGGACAATTTAAATGCAGAGGTGGCACTTGGAACTGTCACCAATGTCAAGGAAGCTTGTGCATGGCTTGGATATACATACCTTTTTATAAGGATGAGGCTCAACCCTTTGGCATATGGTATCGCGTGGGAGGAGGTTATGGCAGATCCTTCCTTGAGTTTAAAGCAGAGATCTCTCGTAGCAGATGCTGCGCGTGCCCTTGACAAAGCAAAAATGATGCGGTTTGACGAGAAAAGTGGCAATTTTTATTGTACAGAGCTTGGTCGCATAGCAAGCCACTTTTATATTCAATACTCTAGTGTTGAAACCTACAATGAAATGTTGAAACGTCACATGAATGAGACTGAGGTAATTGACATGGTAGCCCACTCCTCGGAATTTGAAAATATTGTTATTCGAGAAGAGGAACAAAATGAGCTTGAGATGTTGGTGCGAAAATTGTGTCCAATAGAAGTTAGGGGTGGTCCTTCAAATAAACATGGAAAGATTTCCGTTCTCATTCAGGTGTACATATCTAGAGGCTCAATTGATACCTTTTCTTTGGTTTCTGATGCTCAATATATAAGTGCAAGCTTGGCACGTATTATGCGGGCCTTATTTGAGATTTGTTTGCGAAAAGGCTGGAGTGAGATGTCATTGTTCATGTTGGAATATTGCAAGGCGGTGGACCGCCAAGTTTGGCCCCATCAACATCCTTTCAGACAATTTGACAGGGAGATTTCAGCACAAATACTGCGAAATCTTGAGGAACGAGGTGCTGATCTAGATCGCCTATATGATATGGAGGAAAAAGAGATTGGGAAACTGGTCAATTATGGACCTGGAGGAAGGTTAGCAGCAACTGTAAGTCCAATTACCAGAACTGTTTTAAAGGTGGATTTGCATATTAAACCAGACTTTATATGGAAAGATCGTTTTCACGGCACTGCCCAGCGTTGGTGGATTCTAGTTGAGGATTCTGAGAATGATCATATCTACCACTCGGAGCTATTCACTCTAACAAAGCGGATGGCTAAAGGGGAACCTCAGAAGCTCTCGTTCACTGTGCCAATATTTGAGCCACATCCGCCACAGTATTATATTCGTGCTGTTTCTGATTCTTGGTTGCATGCAGAGGCATTCTACACCATTTCTTTCCATAATCTAACACTACCAGAGGCCCATACTTCACATACAGAACTGCTAGATTTAAAGCCTCTCCCTGTGACATCTCTTGGAAACACTGCTTATGAAGCATTGTACAAATTCTCACACTTTAATCCAATACAAACACAGACTTTCCATGTGCTGTATCACACAGACAATAATGTATTACTGGGAGCTCCTACTGGAAGTGGAAAAACTATTTCTGCTGAGCTTGCTATGCTTCGTCTCTTCAACACCCAGCCTGATATGAAGGTCATTTACATAGCACCTTTGAAGGCTATTGTTCGAGAAAGAATGAACGATTGGAGGAAGCGCCTTGTCTCTCAGCTAGGGAAGAAAATGGTTGAAATGACAGGAGATTATACTCCAGATTTGATGGCCATCTTGTCAGCAGATATCATAATATCAACTCCTGAAAAATGGGATGGTATCAGTCGTAATTGGCATAGCCGAACCTATGTTAAAAAGGTTGGGCTTATGATTTTGGATGAGATTCACTTGTTGGGAGCTGACCGTGGGCCTATCCTCGAGGTTATTGTGTCAAGGATGAGATACATATCCTCACAGACAGAGCGTGAAGTACGGTTTGTAGGCCTTTCTACAGCTCTGGCAAATGCAGGAGATTTAGCTGATTGGTTGGGTGTTGGAGAAACTGGACTCTTCAATTTCAAACCAAGTGTGAGACCTGTACCTCTTGAAGTTCATATCCAGGGATATCCAGGAAAGTTCTATTGTCCAAGGATGAATAGCATGAACAAACCATCATATGCTGCTATATGCACCCATTCACCTACAAAACCGGTTCTAATTTTTGTTTCCTCACGTCGCCAGACAAGGCTTACTGCATTGGACCTCATTCAGTATGCAGCTTCAGATGAGCATCCAAGGCAGTTTCTTTGTATACCAGAAGAAGAACTGCAGATGGTTCTTTATCAGGTTGCTGATAGTAACCTGAGGCACACCTTGCAATTTGGCATTGGATTACACCATGCTGGTCTGAATGACAAAGATAGATCCCTGGTATTGGTTTGTACTAGCACGTTGGCGTGGGGTGTAAACCTTCCAGCGCATCTGGTTATTATTAAGGGAACAGAATTTTATGATGGGAAATCAAAAAGATATGTCGACTTCCCAATCACAGATATCTTGCAAATGATGGGTCGTGCAGGTCGCCCACAATTTGATCAACACGGGAAAGCAGTCATTCTTGTTCATGAACCCAAAAAGAGCTTCTATAAAAAGTTTTTGTATGAACCATTTCCTGTCGAGAGTAGTCTGAGGGAACAGCTGCATAATCACATTAATGCGGAGATAGTTTCTGGCACAATTTGCCATAAAGAGGATGCATTGCATTATCTTACGTGGACATATTTATTCCGCAGACTGATGTTTAATCCGGCTTACTATGGCTTGGACAATACTGATGCCGAAGTTCTCAGTTCTTATTTATCTAGGTTAGTGCAGAACACATTGGAGGATCTGGAAGACAGTGGATGCATCAAGATGAGTGAAGACAGTGTGGAGCCAATGATGCTGGGTTCAATAGCATCCCAGTATTACCTTAGCTACTTGACTGTATCGATGTTTGGTTCAAACATTGGCTCAGATACCTCTCTTGAAGTATTCTTGCATATCCTATCTGCTGCTTCTGAATATGATGAGCTTCCTGTTCGCCATAACGAGGAAAATTACAATGAGGCATTGTCCGAGAGAGTTCGATATAAAGTGGATAAGGATCGCCTAGATGATCCACATGTCAAAGCAAATCTGCTATTTCAGGCACATTTCTCGCAGTTAGAATTACCAATTACTGACTATGTCACAGACTTGAAGTCAGTTTTGGATCAAAGTATACGGATTATCCAGGCCATGATAGATATATGTGCCAACAGTGGGTGGCTTTCAAGTTCAGTTACTTGCATGCATCTTTTGCAGATGGTCATGCAGGGCTTATGGTTTGATGAGGATTCTTCCTTATGGATGTTGCCTTGCATGAATGTTGAACTTGCGGAATCACTCGGCAAACGAGGAATCTTCCATATACAGCAGCTATTGGAACTTCCCAAGGCAACTTTGCAGAACATGATTGGAAACTTCCCTGCTTCAAAATTCTTTCAGGATCTGCAGCTCTTTCCACGCATCGAAGTCAAGTTAAAAATTCTACGGAAAGATGGTGGAAAATCCAGCTCTTTAAACATCAGATTGGTGAAGACCAATTTCAGGAAATATAAATCAAGAGCATTCACTCCACGTTTTCCTAAGGTGAAAAATGAAGCATGGTGGTTGGTCCTTGGGAACACCTCTACGTTGGAACTATATGCTCTGAAGAGAGTATCTTTCTCTGATCACTTGGTTACTAACATGGAGTTACCATCCGATTCAACCACTCTCCAGTTTGTGGAATTGTCTTGCTTTATCAGGGATGAAACTGATTTTAATATCAGATTGTTATCTGGGGTTCGAACAAGAGCATTCAATTTCAGAACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DEAD Helicase_C Sec63

Protein Analysis

2083

Amino Acids

236.19

Weight (kDa)

6.11

Isoelectric Point (pI)

41.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DExH14_plug PF24557 45 - 145 1.3e-41 DExH14 plug domain
DEAD PF00270 440 - 614 1.5e-30 DEAD/DEAH box helicase
ResIII PF04851 451 - 613 3.2e-12 Type III restriction enzyme, res subunit
Helicase_C PF00271 665 - 792 1.3e-09 Helicase conserved C-terminal domain
WHD_SNRNP200 PF23445 868 - 934 5.9e-13 Winged Helix-turn-helix domain
Sec63 PF02889 956 - 1250 1.2e-85 Sec63 Brl domain
DEAD PF00270 1292 - 1458 2.6e-28 DEAD/DEAH box helicase
ResIII PF04851 1300 - 1425 6.6e-11 Type III restriction enzyme, res subunit
WHD_SNRNP200 PF23445 1677 - 1757 8.1e-32 Winged Helix-turn-helix domain
Sec63 PF02889 1763 - 2046 7e-60 Sec63 Brl domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 2405, 2507, 2705
AarI CACCTGC 1 cut(s) 788
AatII GACGTC 1 cut(s) 54
AbsI CCTCGAGG 1 cut(s) 4289
Acc36I ACCTGC 3 cut(s) 788, 1470, 4919
AccB1I GGYRCC 1 cut(s) 506
AccB7I CCANNNNNTGG 2 cut(s) 2312, 6160
AccI GTMKAC 4 cut(s) 54, 4663, 4887, 5614
AccII CGCG 3 cut(s) 19, 2741, 2809
AcoI YGGCCR 2 cut(s) 750, 4140
AcuI CTGAAG 2 cut(s) 4617, 6107
AcyI GRCGYC 1 cut(s) 51
AflIII ACRYGT 2 cut(s) 1677, 5515
AgeI ACCGGT 1 cut(s) 4561
AhdI GACNNNNNGTC 2 cut(s) 919, 5593
AhlI ACTAGT 3 cut(s) 898, 1372, 1762
AjiI CACGTC 2 cut(s) 2412, 4586
AleI CACNNNNGTG 1 cut(s) 2276
AloI GAACNNNNNNTCC 2 cut(s) 2155, 2187
Alw21I GWGCWC 4 cut(s) 122, 1234, 1384, 3940
AlwNI CAGNNNCTG 7 cut(s) 983, 1394, 1864, 2086, 2804, 3458, 5884
Ama87I CYCGRG 1 cut(s) 4289
ArsI GACNNNNNNTTYG 4 cut(s) 3043, 3075, 4906, 4938
AseI ATTAAT 1 cut(s) 5064
AsiGI ACCGGT 1 cut(s) 4561
Asp700I GAANNNNTTC 3 cut(s) 840, 3103, 5019
AspLEI GCGC 7 cut(s) 75, 666, 1011, 2196, 2809, 4077, 4831
AsuC2I CCSGG 1 cut(s) 1748
AsuII TTCGAA 1 cut(s) 6223
AvaI CYCGRG 1 cut(s) 4289
AxyI CCTNAGG 2 cut(s) 4715, 6021
BaeGI GKGCMC 3 cut(s) 372, 1923, 2815
BalI TGGCCA 2 cut(s) 752, 4142
BanI GGYRCC 1 cut(s) 506
BanII GRGCYC 3 cut(s) 45, 122, 3940
BarI GAAGNNNNNNTAC 2 cut(s) 4004, 4036
BbsI GAAGAC 4 cut(s) 1785, 5253, 5280, 5978
Bbv12I GWGCWC 4 cut(s) 122, 1234, 1384, 3940
BbvCI CCTCAGC 1 cut(s) 2087
BceAI ACGGC 3 cut(s) 1004, 2091, 3553
BcgI CGANNNNNNTGC 2 cut(s) 5532, 5566
BciVI GTATCC 1 cut(s) 1298
BclI TGATCA 5 cut(s) 442, 700, 3583, 4945, 6106
BcnI CCSGG 1 cut(s) 1748
BcuI ACTAGT 3 cut(s) 898, 1372, 1762
BfmI CTRYAG 4 cut(s) 1515, 4362, 4679, 5885
BfoI RGCGCY 2 cut(s) 2197, 4078
BfuAI ACCTGC 3 cut(s) 788, 1470, 4919
BfuI GTATCC 1 cut(s) 1298
BglII AGATCT 3 cut(s) 1263, 2116, 2786
BlpI GCTNAGC 1 cut(s) 3969
BmcAI AGTACT 1 cut(s) 718
BmeRI GACNNNNNGTC 2 cut(s) 919, 5593
BmeT110I CYCGRG 1 cut(s) 4289
BmgBI CACGTC 2 cut(s) 2412, 4586
BmiI GGNNCC 7 cut(s) 42, 508, 2290, 2291, 3299, 3639, 5286
BmrI ACTGGG 2 cut(s) 3941, 5308
BmuI ACTGGG 2 cut(s) 3941, 5308
BpiI GAAGAC 4 cut(s) 1785, 5253, 5280, 5978
BpmI CTGGAG 6 cut(s) 1266, 1602, 3259, 3459, 4113, 6137
Bpu10I CCTNAGC 2 cut(s) 2087, 5324
Bpu1102I GCTNAGC 1 cut(s) 3969
Bpu14I TTCGAA 1 cut(s) 6223
BpuEI CTTGAG 6 cut(s) 655, 717, 1272, 2791, 3056, 3380
BpuMI CCSGG 1 cut(s) 1748
Bsa29I ATCGAT 2 cut(s) 672, 5342
BsaAI YACGTR 2 cut(s) 3197, 5124
BsaBI GATNNNNATC 9 cut(s) 1059, 1262, 1309, 3381, 4142, 4318, 4641, 5496, 6211
BsaHI GRCGYC 1 cut(s) 51
BsaI GGTCTC 3 cut(s) 2319, 2365, 4438
BsaWI WCCGGW 2 cut(s) 301, 4561
BsaXI ACNNNNNCTCC 2 cut(s) 207, 237
Bse118I RCCGGY 2 cut(s) 285, 4561
Bse1I ACTGG 9 cut(s) 747, 1585, 1916, 3426, 3936, 3949, 4420, 5314, 6154
Bse21I CCTNAGG 2 cut(s) 4715, 6021
Bse3DI GCAATG 2 cut(s) 2043, 5108
Bse8I GATNNNNATC 9 cut(s) 1059, 1262, 1309, 3381, 4142, 4318, 4641, 5496, 6211
BseCI ATCGAT 2 cut(s) 672, 5342
BseJI GATNNNNATC 9 cut(s) 1059, 1262, 1309, 3381, 4142, 4318, 4641, 5496, 6211
BseMI GCAATG 2 cut(s) 2043, 5108
BseNI ACTGG 9 cut(s) 747, 1585, 1916, 3426, 3936, 3949, 4420, 5314, 6154
BseRI GAGGAG 5 cut(s) 269, 320, 1217, 2762, 2977
BseSI GKGCMC 3 cut(s) 372, 1923, 2815
BseYI CCCAGC 5 cut(s) 2159, 2366, 3546, 3999, 5296
BsgI GTGCAG 3 cut(s) 1448, 4947, 5243
Bsh1236I CGCG 3 cut(s) 19, 2741, 2809
BshNI GGYRCC 1 cut(s) 506
BshTI ACCGGT 1 cut(s) 4561
BshVI ATCGAT 2 cut(s) 672, 5342
BsiHKAI GWGCWC 4 cut(s) 122, 1234, 1384, 3940
BsiHKCI CYCGRG 1 cut(s) 4289
BsiSI CCGG 7 cut(s) 286, 302, 336, 530, 1748, 4562, 5159
BslFI GGGAC 2 cut(s) 1833, 2302
BsmBI CGTCTC 3 cut(s) 411, 2418, 3992
BsmFI GGGAC 2 cut(s) 1833, 2302
BsmI GAATGC 5 cut(s) 1323, 2340, 3734, 6002, 6233
Bso31I GGTCTC 3 cut(s) 2319, 2365, 4438
BsoBI CYCGRG 1 cut(s) 4289
Bsp119I TTCGAA 1 cut(s) 6223
Bsp1286I GDGCHC 8 cut(s) 45, 122, 372, 1234, 1384, 1923, 2815, 3940
Bsp1407I TGTACA 4 cut(s) 2231, 2867, 3123, 3855
Bsp1720I GCTNAGC 1 cut(s) 3969
BspDI ATCGAT 2 cut(s) 672, 5342
BspFNI CGCG 3 cut(s) 19, 2741, 2809
BspHI TCATGA 3 cut(s) 487, 2416, 4975
BspLI GGNNCC 7 cut(s) 42, 508, 2290, 2291, 3299, 3639, 5286
BspMAI CTGCAG 3 cut(s) 1519, 4683, 5889
BspMI ACCTGC 3 cut(s) 788, 1470, 4919
BspQI GCTCTTC 1 cut(s) 1167
BspT104I TTCGAA 1 cut(s) 6223
BspT107I GGYRCC 1 cut(s) 506
BspTNI GGTCTC 3 cut(s) 2319, 2365, 4438
BsrDI GCAATG 2 cut(s) 2043, 5108
BsrFI RCCGGY 2 cut(s) 285, 4561
BsrGI TGTACA 4 cut(s) 2231, 2867, 3123, 3855
BsrI ACTGG 9 cut(s) 747, 1585, 1916, 3426, 3936, 3949, 4420, 5314, 6154
BssAI RCCGGY 2 cut(s) 285, 4561
BssNAI GTATAC 2 cut(s) 4664, 5615
BssNI GRCGYC 1 cut(s) 51
BssT1I CCWWGG 6 cut(s) 555, 1135, 4499, 4646, 5826, 6052
Bst1107I GTATAC 2 cut(s) 4664, 5615
Bst6I CTCTTC 6 cut(s) 1167, 1895, 3012, 3995, 4427, 6083
BstACI GRCGYC 1 cut(s) 51
BstAPI GCANNNNNTGC 7 cut(s) 352, 1514, 1766, 2804, 4726, 5091, 5692
BstAUI TGTACA 4 cut(s) 2231, 2867, 3123, 3855
BstBAI YACGTR 2 cut(s) 3197, 5124
BstBI TTCGAA 1 cut(s) 6223
BstDSI CCRYGG 1 cut(s) 4276
BstEII GGTNACC 1 cut(s) 1457
BstFNI CGCG 3 cut(s) 19, 2741, 2809
BstH2I RGCGCY 2 cut(s) 2197, 4078
BstHHI GCGC 7 cut(s) 75, 666, 1011, 2196, 2809, 4077, 4831
BstNSI RCATGY 8 cut(s) 394, 699, 946, 1591, 1681, 3728, 5519, 5686
BstPI GGTNACC 1 cut(s) 1457
BstSFI CTRYAG 4 cut(s) 1515, 4362, 4679, 5885
BstSLI GKGCMC 3 cut(s) 372, 1923, 2815
BstUI CGCG 3 cut(s) 19, 2741, 2809
BstV2I GAAGAC 4 cut(s) 1785, 5253, 5280, 5978
BstX2I RGATCY 8 cut(s) 482, 1263, 2116, 2761, 2786, 4775, 5239, 5881
BstXI CCANNNNNNTGG 1 cut(s) 1846
BstYI RGATCY 8 cut(s) 482, 1263, 2116, 2761, 2786, 4775, 5239, 5881
BstZ17I GTATAC 2 cut(s) 4664, 5615
Bsu15I ATCGAT 2 cut(s) 672, 5342
Bsu36I CCTNAGG 2 cut(s) 4715, 6021
BsuI GTATCC 1 cut(s) 1298
BsuTUI ATCGAT 2 cut(s) 672, 5342
BtgI CCRYGG 1 cut(s) 4276
BtrI CACGTC 2 cut(s) 2412, 4586
BtsI GCAGTG 4 cut(s) 322, 1678, 3540, 3837
BveI ACCTGC 3 cut(s) 788, 1470, 4919
CaiI CAGNNNCTG 7 cut(s) 983, 1394, 1864, 2086, 2804, 3458, 5884
CciI TCATGA 3 cut(s) 487, 2416, 4975
CfoI GCGC 7 cut(s) 75, 666, 1011, 2196, 2809, 4077, 4831
Cfr10I RCCGGY 2 cut(s) 285, 4561
ClaI ATCGAT 2 cut(s) 672, 5342
CseI GACGC 1 cut(s) 393
CsiI ACCWGGT 1 cut(s) 781
CspAI ACCGGT 1 cut(s) 4561
DraI TTTAAA 5 cut(s) 2628, 2778, 3486, 3807, 5955
DriI GACNNNNNGTC 2 cut(s) 919, 5593
EaeI YGGCCR 2 cut(s) 750, 4140
Eam1104I CTCTTC 6 cut(s) 1167, 1895, 3012, 3995, 4427, 6083
Eam1105I GACNNNNNGTC 2 cut(s) 919, 5593
EarI CTCTTC 6 cut(s) 1167, 1895, 3012, 3995, 4427, 6083
EciI GGCGGA 2 cut(s) 1816, 3672
Ecl136II GAGCTC 2 cut(s) 120, 3938
Eco130I CCWWGG 6 cut(s) 555, 1135, 4499, 4646, 5826, 6052
Eco147I AGGCCT 5 cut(s) 62, 472, 1134, 1887, 4356
Eco24I GRGCYC 3 cut(s) 45, 122, 3940
Eco31I GGTCTC 3 cut(s) 2319, 2365, 4438
Eco32I GATATC 3 cut(s) 4159, 4478, 4906
Eco53kI GAGCTC 2 cut(s) 120, 3938
Eco57I CTGAAG 2 cut(s) 4617, 6107
Eco81I CCTNAGG 2 cut(s) 4715, 6021
Eco88I CYCGRG 1 cut(s) 4289
Eco91I GGTNACC 1 cut(s) 1457
EcoICRI GAGCTC 2 cut(s) 120, 3938
EcoO109I RGGNCCY 3 cut(s) 847, 1207, 2289
EcoO65I GGTNACC 1 cut(s) 1457
EcoRI GAATTC 3 cut(s) 2135, 2148, 2588
EcoRV GATATC 3 cut(s) 4159, 4478, 4906
EcoT14I CCWWGG 6 cut(s) 555, 1135, 4499, 4646, 5826, 6052
EcoT22I ATGCAT 4 cut(s) 697, 5110, 5261, 5688
EcoT38I GRGCYC 3 cut(s) 45, 122, 3940
ErhI CCWWGG 6 cut(s) 555, 1135, 4499, 4646, 5826, 6052
Esp3I CGTCTC 3 cut(s) 411, 2418, 3992
FalI AAGNNNNNCTT 4 cut(s) 1710, 1742, 2552, 2584
FaqI GGGAC 2 cut(s) 1833, 2302
FauI CCCGC 1 cut(s) 3198
FauNDI CATATG 3 cut(s) 619, 2731, 4528
FbaI TGATCA 5 cut(s) 442, 700, 3583, 4945, 6106
FblI GTMKAC 4 cut(s) 54, 4663, 4887, 5614
FriOI GRGCYC 3 cut(s) 45, 122, 3940
GlaI GCGC 7 cut(s) 74, 665, 1010, 2195, 2808, 4076, 4830
GsaI CCCAGC 5 cut(s) 2163, 2370, 3550, 4003, 5300
GsuI CTGGAG 6 cut(s) 1266, 1602, 3259, 3459, 4113, 6137
HaeII RGCGCY 2 cut(s) 2197, 4078
HapII CCGG 7 cut(s) 286, 302, 336, 530, 1748, 4562, 5159
HgaI GACGC 1 cut(s) 393
HhaI GCGC 7 cut(s) 75, 666, 1011, 2196, 2809, 4077, 4831
Hin1I GRCGYC 1 cut(s) 51
Hin6I GCGC 7 cut(s) 73, 664, 1009, 2194, 2807, 4075, 4829
HinP1I GCGC 7 cut(s) 73, 664, 1009, 2194, 2807, 4075, 4829
HincII GTYRAC 4 cut(s) 55, 388, 2456, 4888
HindII GTYRAC 4 cut(s) 55, 388, 2456, 4888
HindIII AAGCTT 4 cut(s) 195, 1001, 2670, 3187
HpaI GTTAAC 1 cut(s) 388
HpaII CCGG 7 cut(s) 286, 302, 336, 530, 1748, 4562, 5159
Hpy99I CGWCG 3 cut(s) 53, 56, 4590
Hsp92I GRCGYC 1 cut(s) 51
HspAI GCGC 7 cut(s) 73, 664, 1009, 2194, 2807, 4075, 4829
KflI GGGWCCC 1 cut(s) 2289
Ksp22I TGATCA 5 cut(s) 442, 700, 3583, 4945, 6106
KspAI GTTAAC 1 cut(s) 388
LguI GCTCTTC 1 cut(s) 1167
MabI ACCWGGT 1 cut(s) 781
MfeI CAATTG 1 cut(s) 3141
MflI RGATCY 8 cut(s) 482, 1263, 2116, 2761, 2786, 4775, 5239, 5881
MhlI GDGCHC 8 cut(s) 45, 122, 372, 1234, 1384, 1923, 2815, 3940
MlsI TGGCCA 2 cut(s) 752, 4142
MluNI TGGCCA 2 cut(s) 752, 4142
MlyI GAGTC 5 cut(s) 140, 578, 1799, 2021, 4413
MmeI TCCRAC 7 cut(s) 114, 789, 1244, 1409, 3243, 4387, 6051
Mox20I TGGCCA 2 cut(s) 752, 4142
Mph1103I ATGCAT 4 cut(s) 697, 5110, 5261, 5688
MroXI GAANNNNTTC 3 cut(s) 840, 3103, 5019
MscI TGGCCA 2 cut(s) 752, 4142
MslI CAYNNNNRTG 7 cut(s) 492, 1412, 2276, 2952, 3119, 5064, 5264
Msp20I TGGCCA 2 cut(s) 752, 4142
MspA1I CMGCKG 2 cut(s) 590, 5050
MspI CCGG 7 cut(s) 286, 302, 336, 530, 1748, 4562, 5159
MunI CAATTG 1 cut(s) 3141
Mva1269I GAATGC 5 cut(s) 1323, 2340, 3734, 6002, 6233
MvnI CGCG 3 cut(s) 19, 2741, 2809
NciI CCSGG 1 cut(s) 1748
NdeI CATATG 3 cut(s) 619, 2731, 4528
NlaIV GGNNCC 7 cut(s) 42, 508, 2290, 2291, 3299, 3639, 5286
NmeAIII GCCGAG 1 cut(s) 5760
NsiI ATGCAT 4 cut(s) 697, 5110, 5261, 5688
NspI RCATGY 8 cut(s) 394, 699, 946, 1591, 1681, 3728, 5519, 5686
NspV TTCGAA 1 cut(s) 6223
OliI CACNNNNGTG 1 cut(s) 2276
PaeI GCATGC 3 cut(s) 946, 3728, 5686
PaeR7I CTCGAG 1 cut(s) 4289
PagI TCATGA 3 cut(s) 487, 2416, 4975
PaqCI CACCTGC 1 cut(s) 788
PceI AGGCCT 5 cut(s) 62, 472, 1134, 1887, 4356
PciI ACATGT 2 cut(s) 1677, 5515
PciSI GCTCTTC 1 cut(s) 1167
PcsI WCGNNNNNNNCGW 1 cut(s) 140
PctI GAATGC 5 cut(s) 1323, 2340, 3734, 6002, 6233
PdmI GAANNNNTTC 3 cut(s) 840, 3103, 5019
PflFI GACNNNGTC 1 cut(s) 5579
PflMI CCANNNNNTGG 2 cut(s) 2312, 6160
PfoI TCCNGGA 1 cut(s) 4480
PinAI ACCGGT 1 cut(s) 4561
PleI GAGTC 5 cut(s) 139, 577, 1798, 2020, 4413
PpsI GAGTC 5 cut(s) 139, 577, 1798, 2020, 4413
Ppu21I YACGTR 2 cut(s) 3197, 5124
PpuMI RGGWCCY 3 cut(s) 847, 1207, 2289
PscI ACATGT 2 cut(s) 1677, 5515
PshBI ATTAAT 1 cut(s) 5064
PsiI TTATAA 3 cut(s) 2405, 2507, 2705
Psp124BI GAGCTC 2 cut(s) 122, 3940
Psp5II RGGWCCY 3 cut(s) 847, 1207, 2289
PspEI GGTNACC 1 cut(s) 1457
PspFI CCCAGC 5 cut(s) 2159, 2366, 3546, 3999, 5296
PspN4I GGNNCC 7 cut(s) 42, 508, 2290, 2291, 3299, 3639, 5286
PspPPI RGGWCCY 3 cut(s) 847, 1207, 2289
PspXI VCTCGAGB 1 cut(s) 4289
PstI CTGCAG 3 cut(s) 1519, 4683, 5889
PstNI CAGNNNCTG 7 cut(s) 983, 1394, 1864, 2086, 2804, 3458, 5884
PsuI RGATCY 8 cut(s) 482, 1263, 2116, 2761, 2786, 4775, 5239, 5881
PsyI GACNNNGTC 1 cut(s) 5579
PvuII CAGCTG 1 cut(s) 5050
RseI CAYNNNNRTG 7 cut(s) 492, 1412, 2276, 2952, 3119, 5064, 5264
SacI GAGCTC 2 cut(s) 122, 3940
SalI GTCGAC 2 cut(s) 53, 4886
SapI GCTCTTC 1 cut(s) 1167
ScaI AGTACT 1 cut(s) 718
SchI GAGTC 5 cut(s) 140, 578, 1799, 2021, 4413
SduI GDGCHC 8 cut(s) 45, 122, 372, 1234, 1384, 1923, 2815, 3940
SexAI ACCWGGT 1 cut(s) 781
SfcI CTRYAG 4 cut(s) 1515, 4362, 4679, 5885
Sfr274I CTCGAG 1 cut(s) 4289
SfuI TTCGAA 1 cut(s) 6223
SlaI CTCGAG 1 cut(s) 4289
SmiI ATTTAAAT 1 cut(s) 2628
SmiMI CAYNNNNRTG 7 cut(s) 492, 1412, 2276, 2952, 3119, 5064, 5264
SmlI CTYRAG 7 cut(s) 634, 732, 1287, 2770, 3035, 3359, 4289
SmoI CTYRAG 7 cut(s) 634, 732, 1287, 2770, 3035, 3359, 4289
SpeI ACTAGT 3 cut(s) 898, 1372, 1762
SphI GCATGC 3 cut(s) 946, 3728, 5686
SseBI AGGCCT 5 cut(s) 62, 472, 1134, 1887, 4356
SspI AATATT 5 cut(s) 1114, 2539, 3004, 3269, 3669
SstI GAGCTC 2 cut(s) 122, 3940
StuI AGGCCT 5 cut(s) 62, 472, 1134, 1887, 4356
StyI CCWWGG 6 cut(s) 555, 1135, 4499, 4646, 5826, 6052
SwaI ATTTAAAT 1 cut(s) 2628
TatI WGTACW 7 cut(s) 716, 1350, 2231, 2867, 3123, 3855, 4793
TauI GCSGC 2 cut(s) 193, 1335
TspGWI ACGGA 3 cut(s) 3097, 5632, 5941
Tth111I GACNNNGTC 1 cut(s) 5579
Van91I CCANNNNNTGG 2 cut(s) 2312, 6160
VspI ATTAAT 1 cut(s) 5064
XbaI TCTAGA 2 cut(s) 3131, 3379
XceI RCATGY 8 cut(s) 394, 699, 946, 1591, 1681, 3728, 5519, 5686
XcmI CCANNNNNNNNNTGG 1 cut(s) 3516
XhoI CTCGAG 1 cut(s) 4289
XmiI GTMKAC 4 cut(s) 54, 4663, 4887, 5614
XmnI GAANNNNTTC 3 cut(s) 840, 3103, 5019
ZraI GACGTC 1 cut(s) 52
ZrmI AGTACT 1 cut(s) 718
Zsp2I ATGCAT 4 cut(s) 697, 5110, 5261, 5688
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.