MD06G1201900.v1.1

Activating signal cointegrator 1 complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0008552
Physical Location & Seq
Reverse (-)
33508489 .. 33525535
17047 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1201900.v1.1.491

Sequence Viewer

Length: 6096 bp
ATGTTGGTTCAACTTCCGCGGCTGACCAGCTCTCTCAGAGAGCCCTTTGACGTCGATCAGGCCTATTTGCAGCGGAAGATCCTCCTCCAGAGCCGCCACAAACCGCCCCAGTCTTCAAGCTCAGTTGATGAGTCGGAACTTGCTAGGAAGATAGTTCATAGATGGGAAGAAGCTTCGGTTGAAGTGCGGCAAGCGTACAAGCAGTTTGTCGGAGCAGTGGTTGAATTGATAGATGGAGAAGTTTCCTCTGAAGAGTTTCGTGAGGTGGCACTGGCTATGTATCGCCTTTTCAGCTCGCCAGCGGAGGAAGCCAATGTTGAAACAATTATTGCTAGGGAAAAGCTGGAAGTGCAAAACTTACTTGGTCAAGCCGTTTCGGATGCTAACATGCGGAAAGTTGTCTCCCTTGCCCAGAGACTTTGTGGGATGCAGTCTAGTGATGGGGGGACTGCGCTTCCAGAAAACCCTGTAAGTGGGACTGATGACAATGCAGAATTTGGCGCTGACCTTGTTTTCCATGCACCAGCTCGGTTTCTGGTGGATGTTTCTTTGGAGGATGGGGAGCTATTGGGTGAGGAAAGCACTTCTTCTACATCATATTATGAAGGATTATATAGTCGCAATGACTTGAATGATCATCAACCTACTTCCAATGGACAAAGCTTCAACTTGAGTTGGTTACAAGATGCATGTGATCGGATAATAACAAAAAGTACTTCACAGCTTTCTGGAGATGAATTGGCTATGGCCATCTGTCGGGTTCTTGACTCTGACAAATCTGGTGATGAGATAGCAGGTGACTTGTTAGATCTTGTTGGGGATAGTGCGTTTGAAACTGTCCAAGACCTCATATCACATCGAAAGGAACTTGTTGATGCCATCCATCAAGGGCTACTGGGACTGAAATCTGATAAATTATCTTCAAGCTCTCAACCACGTATGCCTAGTTATGGCACACAGGTTACTGTTCAAACAGAATCTGAACGGCAAATTGATAAACTTCGGCGCAAAGAGGAAAAGCGACAAAGACGAGGAACAGAATATGGCGCTGATAGTGATCTGGCTGGTGTAAATTTTTCTTCTCTAGTACAAGCAAGTGAAAGAAAAAAACCTGTTGATGGTCTCTTAGGGTACGGTGAAGCTCACTCTTTAGCAGTTAGTGCTCTTCCTCAAGGAACAGTGAGGAAGCATCATAAGGGGTATGAGGAAGTCATTATTCCCCCAACACCAACAGCACAAATGAAACCAGGAGAAAAGCTGATTGACATAACAGAGTTAGATGAATTTGCTCAAGCTGCTTTTCGGGGATACAAATCTTTGAACCGTATTCAGAGCATCATATTTCATACTGTTTATAACACTAATGAAAATATACTAGTTTGTGCTCCAACAGGAGCTGGTAAGACGAACATAGCTATGATTTCTATTCTACATGAGATTGGACAGCACTTCAAAGATGGTTACTTGCATAAAGATGAATTTAAAATAGTTTATGTTGCACCTATGAAGGCATTAGCTTCAGAAGTCACATCAACCTTTAGCCATCGCTTATCTCCATTGAATATGACTGTGAAAGAACTCACCGGCGACATGCAACTATCTAAGAATGAACTTGAAGAAACTCAGATGATAGTTACTACACCTGAAAAATGGGATGTCATTACTCGCAAGAGCAGTGACATGGCACTTTCAATGCTCGTGAAGCTTTTAATTATTGATGAAGTGCATCTATTGAATGATGACAGGGGCCCTGTAATTGAGGCACTAGTTGCTCGGACTTTGCGCCAGGTGGAATCTACACAGACGATGATACGCATTGTTGGCCTTTCAGCCACTCTACCCAACTATTTGGAGGTTGCACAGTTTCTGAGAGTGAATCCAGATGCAGGACTCTTCTTTTTTGATGCTAGTTATCGTCCTGTGCCCCTTGCACAGCAGTATATTGGTATTAGTGAGCAGAACTTTGCAGCCCGAAATGAATTAATGAATGAGATATGCTACAAGAAGGTTGTTGAATCACTCAGGCAAGGGTATCAGGCAATGGTATTCGTTCATTCTCGAAAGGACACAGCAAAAACAGCTCAGAAATTGGTTGAACTTGCTCGCAAGTTTGAAGGACTGGATCTTTTCAAGAACGATGAACACCCACAGTTTTCATTGGTTCAGAGGGATGTTAAGAAGTCCAGAAACAAAGATCTTGTTGCACTTTTTGAATTTGGAGTTGGAGTTCATCATGCTGGGATGTTACGGTCTGATAGAGGTCTGACTGAGCGCCTTTTCTCTGATGGACTATTGAAGGTGCTTGTTTGTACAGCAACTTTGGCATGGGGTGTAAATCTACCAGCTCACACTGTTGTGATAAAGGGGACTCAATTGTATGATCCAAAGGCTGGTGGGTGGAAAGACCTGGGCATGCTAGATGTTATGCAGATCTTTGGGCGTGCTGGGAGACCTCAATTCGACAAAAGTGGTGAAGGCATTATAATTACGTCTCACGACAAACTGGCCTACTATCTTCGGTTGCTGACAAGTCAACTTCCTATAGAAAGTCAGTTTATTAGTTCCTTGAAAGACAATTTAAATGCAGAGGTGGCACTTGGTACTGTCACCAATGTCAAGGAAGCTTGTGCATGGCTTGGATATACATACCTTTTTATAAGAATGAGGCTGAACCCTTTGGTGTACGGTATCGGGTGGGATGAGGTTGTTGCAGATCCTTCCTTGGGCTTGAAGCAAAGATCTCTGATTGCAGATGCTGCACGTTCCCTGGACAAAGCAAAAATGATGCGATTTGATGAAAAAAGTGGCAACTTTTATTGTACAGAACTTGGTCGAATTGCAAGCCACTTTTATATTCAATACTCAAGTGTTGAAACGTACAATGAATGTTTGAGACGCCACATGAATGAGACTGAGGTAATTGATATGGTAGCCCACTCCTCAGAATTTGAAAATATTGTTGTTCGAGATGAGGAACAGCATGAACTTGAGGCGTTGGTGCGATCATCATGCCCTTTAGAAGTTAAGGGGGGTCCTTCAAATAAACATGGAAAGATTTCCATTCTCATTCAGTTGTACATATCTCGTGGCTCAATCGATACCTTTTCCTTGGTTTCTGATGCTGCATATATAAGTGCAAGCTTGGCACGCATTATGCGGGCTTTATTTGAGATTTGCTTACGAAAAGGCTGGTCTGAGATGTCGTTGTTCATGTTGGACTATTGTAAGGCTGTGGATCGCCAAGTTTGGCCCCATCAGCATCCTCTCAGACAATTTGACAAGGATCTTTCAGGAGAGATACTGCGAAAGCTTGAAGAACAAGAGGCTGACTTAGATCGCCTATATGAGATGGAGGAAAAAGACATTGGAGCATTGATTCGCTATGGGCCTGGAGGAAGGTTGGTCAAGCAATATTTGGGATATTTTCCATGGATCCAGTTGTCAGCAACTGTGAGTCCAATCACCAGAACTGTTTTAAAGGTGGATTTACTTATTACACCAGACTTCATATGGAAAGATCGTTTTCATGGCACATCCCAACGTTGGTGGATTCTCGTTGAGGATTCGGAGAATGACCACATCTACCATTCAGAGCTATTTACTCTGACAAAGCGGATGGCTAAGGGGGAACCTCAGAAGCTTTCTTTTACTGTGCCAATATTTGAGCCACATCCACCACAGTATTATATCCGTGCTGTTTCGGATTCTTGGTTGCGTTCAGAGGCTTTCTACACCATATCTTTCCAGAATCTAGCACTGCCAGAGACTTTCCATGTCTTGTATCACACAGACAATAATGTTTTGTTAGGAGCTCCCACTGGAAGTGGGAAAACTATATCTGCCGAGCTTGCTATGCTTCGTCTCTTCAATACCCAGCCTGATATGAAGGTCATTTACATAGCACCTTTGAAGGCTATTGTTCGGGAAAGAATGAATGATTGGAAAAGACGCCTTGTCTCTCAGCTAGGGAAAGAGATGGTTGAAATGACTGGTGATTATACTCCAGATATGATGGCCATTATGTCAGCGGATATCATAATATCTACTCCTGAGAAGTGGGATGGTATCAGCCGTAATTGGCATAGTCGAGACTATGTTAAGAAGGTTGGGCTTATGATCATGGATGAAATTCACTTGTTGGGAGCTGACCGTGGACCCATCCTTGAGGTTATTGTGTCAAGGATGAGATACATATCATCACAGACAGAGCGTGAAGTACGATTTGTAGGCCTTTCTACAGCTTTAGCAAATGCAGGGGATTTGGCTGATTGGTTGGGTGTTGGGGAAATTGGGCTCTTCAATTTTAAACCAAGCGTGAGACCTGTACCTCTTGAAGTTCATATCCAGGGATACCCTGGAAAGTTCTATTGCCCAAGGATGAATAGCATGAACAAACCAGCATACGCTGCGATATGCACCCACTCACCTACAAAACCAGTTTTAATATTTGTTTCTTCTCGTCGACAGACAAGGCTTACTGCATTGGACCTCATTCAGTTTGCAGCTTCAGATGAACATCCAAGGCAGTTTCTTAGTATGCCAGAAGACGACCTGCAGATGGTGCTTTCTCAGGTATTGGTTTGTACGAGCACATTGGCATGGGGTGTAAACCTTCCAGCTCATCTAGTCATCATTAAGGTATCTGTTGCATCAGCTGTTCTTCCTAGATCTTTCATTTACATAATGCCATTAATTCTCTTGCAGGGAACAGAATATTATGATGGGAAAGCAAAAAGATATGTTGATTTTCCAATCACAGATATCTTGCAAATGATGGGTCGTGCTGGCCGGCCACAATTTGATCAGCATGGGAAAGCAGTCATTCTTGTTCATGAACCCAAAAAGAGCTTCTATAAAAAGTTCTTATATGAACCATTTCCCGTCGAGAGTAGTCTGAGGGAACAGTTGCATAATCACATCAACGCTGAGATAGTTTCTGGCACAATTTGCCATAAAGAGGATGCGCTGCATTATCTTACCTGGACATATTTATACCGTAGACTGATGTTTAATCCAGCTTACTATGGCTTGGACAATGCTGAAGCTGAAGTTGTAAATTCTTACTTGTCTAGGCTAGTGCAGAACACATTTGAGGATCTCGAAGACAGTGGATGCATCGAGATGAATGAAGACAGTGTGGAGCCAACGATGCTGGGTTCAATAGCATCCCAGTATTACCTTAGCTACATGACTGTATCAATGTTTGGTTCAAACATAAGTTCAGATACGTCGCTTGAAGTGTTCTTGCATATCCTATCGGCTGCTTCTGAATATAACGAACTTCCTGTGCGGCACAATGAGGAGAATTACAATGGAGCGTTGGCTGAGAGAGTTCGATATAGAGTGGACAAGGACCGGTTAGATGATCCACATGTCAAAGCCAATCTTCTGTTTCAGGCACATTTCTCGCAGTTAGAATTACCAATCAGTGATTACGTCACAGACTTGAAGTCGGTACTGGATCAAAGTATACGGGTCATCCAGGCCATGATAGATATATGTGCCAACAGCGGGTGGCTTTCAAGCTCAGTTACTTGCATGCATCTTCTGCAAATGGTCATGCAGGGTTTATGGTTTGATAAGGATTCTTCCTTGTGGATGATGCCATGCATGAATGTTGAACTCGCCGACTCACTAAGCAAAAGAGGAATATTCAGCGTGCAGCAACTACTGAATCTCCCCAAGGCAACTTTGCAGACCATGATCGGAAACTTCCCGGCTTCAAAGTTTTTTCAGGATCTGCAGCATTTTCCCCGCATCGAAATGAAGTTAAGAATTCAGGAAAAGGACAGCGGAAAGTCTCACTCTTTAAACATCAGATTGGTTAAGACCAATTACAGGCAAAACAAATCAAGAGCGTTTACCCCGCGATTTCCTAAGGTGAAAAACGAAGCGTGGTGGTTGGTCCTTGGGAACACCTCAACATCTGAATTATATGCTCTGAAGAGGGTATCCTTCTCAGATCACTTGGTTACTCACATGGAGTTACCAGCAGCTCCAAATAATATCCAGGGGATGAAATTGATTTTAGTTTCGGATTGTTATTTGGGCTTCGAGCAAGAGCACTCTATCGCGGAACTTATTCAACGGCGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DEAD Helicase_C Sec63

Protein Analysis

2032

Amino Acids

229.8

Weight (kDa)

5.94

Isoelectric Point (pI)

42.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DExH14_plug PF24557 45 - 144 1.4e-42 DExH14 plug domain
DEAD PF00270 441 - 615 1.4e-30 DEAD/DEAH box helicase
ResIII PF04851 447 - 614 5.8e-13 Type III restriction enzyme, res subunit
Helicase_C PF00271 734 - 818 3e-07 Helicase conserved C-terminal domain
WHD_SNRNP200 PF23445 849 - 908 1e-14 Winged Helix-turn-helix domain
Sec63 PF02889 940 - 1247 6.1e-87 Sec63 Brl domain
ResIII PF04851 1249 - 1383 7.1e-12 Type III restriction enzyme, res subunit
DEAD PF00270 1257 - 1416 1.9e-25 DEAD/DEAH box helicase
WHD_SNRNP200 PF23445 1629 - 1710 1.6e-31 Winged Helix-turn-helix domain
Sec63 PF02889 1715 - 2024 7.6e-66 Sec63 Brl domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 1356, 2483, 2657
AarI CACCTGC 1 cut(s) 785
AatII GACGTC 1 cut(s) 54
Acc36I ACCTGC 2 cut(s) 785, 4560
AccB7I CCANNNNNTGG 1 cut(s) 2390
AccI GTMKAC 3 cut(s) 4462, 4999, 5470
AccII CGCG 3 cut(s) 19, 5869, 6074
AclI AACGTT 1 cut(s) 3541
AcoI YGGCCR 4 cut(s) 747, 4014, 4786, 4790
AcsI RAATTY 9 cut(s) 494, 1072, 1283, 1478, 2213, 2945, 4128, 5056, 5775
AcuI CTGAAG 6 cut(s) 270, 1503, 4491, 5061, 5067, 5963
AcyI GRCGYC 3 cut(s) 51, 2896, 3949
AflIII ACRYGT 1 cut(s) 5371
AgeI ACCGGT 1 cut(s) 5355
AhdI GACNNNNNGTC 2 cut(s) 3953, 5449
AhlI ACTAGT 2 cut(s) 1375, 1765
AjuI GAANNNNNNNTTGG 8 cut(s) 2205, 2237, 3348, 3380, 3922, 3954, 4832, 4864
AleI CACNNNNGTG 1 cut(s) 2354
AloI GAACNNNNNNTCC 2 cut(s) 2211, 2243
Alw21I GWGCWC 5 cut(s) 1165, 1387, 3814, 4592, 6066
AlwNI CAGNNNCTG 6 cut(s) 980, 1397, 1867, 2756, 3449, 5740
ApaI GGGCCC 1 cut(s) 1751
ApoI RAATTY 9 cut(s) 494, 1072, 1283, 1478, 2213, 2945, 4128, 5056, 5775
ArsI GACNNNNNNTTYG 2 cut(s) 4762, 4794
AseI ATTAAT 2 cut(s) 1982, 4691
AsiGI ACCGGT 1 cut(s) 5355
Asp700I GAANNNNTTC 4 cut(s) 255, 3055, 4875, 6081
AspLEI GCGC 7 cut(s) 454, 503, 1008, 1049, 1785, 2274, 4966
AspS9I GGNCC 9 cut(s) 1747, 1748, 3032, 3249, 3386, 4154, 4486, 5353, 5905
AsuC2I CCSGG 1 cut(s) 5717
AvaII GGWCC 5 cut(s) 3032, 4154, 4486, 5353, 5905
AxyI CCTNAGG 1 cut(s) 5877
BaeGI GKGCMC 2 cut(s) 1751, 1926
BalI TGGCCA 2 cut(s) 749, 4016
BamHI GGATCC 1 cut(s) 3432
BanII GRGCYC 4 cut(s) 45, 1751, 3814, 4296
BarI GAAGNNNNNNTAC 2 cut(s) 3878, 3910
BauI CACGAG 2 cut(s) 1697, 3084
BbsI GAAGAC 4 cut(s) 105, 4551, 5109, 5136
Bbv12I GWGCWC 5 cut(s) 1165, 1387, 3814, 4592, 6066
BceAI ACGGC 3 cut(s) 356, 1001, 4056
BcgI CGANNNNNNTGC 4 cut(s) 2991, 3025, 5388, 5422
BciVI GTATCC 3 cut(s) 1301, 4343, 5962
BclI TGATCA 3 cut(s) 634, 4116, 4801
BcnI CCSGG 1 cut(s) 5717
BcuI ACTAGT 2 cut(s) 1375, 1765
BfmI CTRYAG 4 cut(s) 2541, 4236, 4553, 5741
BfoI RGCGCY 3 cut(s) 504, 1050, 2275
BfuAI ACCTGC 2 cut(s) 785, 4560
BfuI GTATCC 3 cut(s) 1301, 4343, 5962
BglII AGATCT 5 cut(s) 808, 2194, 2430, 2738, 4667
BmcAI AGTACT 1 cut(s) 715
Bme18I GGWCC 5 cut(s) 3032, 4154, 4486, 5353, 5905
BmeRI GACNNNNNGTC 2 cut(s) 3953, 5449
BmgT120I GGNCC 9 cut(s) 1747, 1748, 3032, 3249, 3386, 4154, 4486, 5353, 5905
BmiI GGNNCC 8 cut(s) 1748, 1749, 3033, 3251, 3434, 3630, 4156, 5142
BmrI ACTGGG 3 cut(s) 103, 905, 5164
BmuI ACTGGG 3 cut(s) 103, 905, 5164
BpiI GAAGAC 4 cut(s) 105, 4551, 5109, 5136
BpmI CTGGAG 4 cut(s) 71, 750, 3411, 3987
Bpu10I CCTNAGC 2 cut(s) 3621, 5180
BpuEI CTTGAG 6 cut(s) 691, 1155, 1275, 2848, 3008, 4184
BpuMI CCSGG 1 cut(s) 5717
Bsa29I ATCGAT 1 cut(s) 3096
BsaAI YACGTR 1 cut(s) 938
BsaBI GATNNNNATC 4 cut(s) 1056, 1312, 4192, 4515
BsaHI GRCGYC 3 cut(s) 51, 2896, 3949
BsaI GGTCTC 3 cut(s) 1127, 2443, 4312
BsaWI WCCGGW 1 cut(s) 5355
BsaXI ACNNNNNCTCC 6 cut(s) 204, 234, 2211, 2241, 5661, 5691
Bse118I RCCGGY 3 cut(s) 1583, 4788, 5355
Bse21I CCTNAGG 1 cut(s) 5877
Bse3DI GCAATG 2 cut(s) 628, 2046
Bse8I GATNNNNATC 4 cut(s) 1056, 1312, 4192, 4515
BseCI ATCGAT 1 cut(s) 3096
BseJI GATNNNNATC 4 cut(s) 1056, 1312, 4192, 4515
BseMI GCAATG 2 cut(s) 628, 2046
BseRI GAGGAG 3 cut(s) 74, 2929, 5315
BseSI GKGCMC 2 cut(s) 1751, 1926
BseYI CCCAGC 4 cut(s) 2237, 2444, 3873, 5152
BsgI GTGCAG 3 cut(s) 2742, 5099, 5681
Bsh1236I CGCG 3 cut(s) 19, 5869, 6074
BshTI ACCGGT 1 cut(s) 5355
BshVI ATCGAT 1 cut(s) 3096
BsiHKAI GWGCWC 5 cut(s) 1165, 1387, 3814, 4592, 6066
BsiSI CCGG 4 cut(s) 1584, 4789, 5356, 5717
BslFI GGGAC 4 cut(s) 460, 490, 912, 2380
BsmBI CGTCTC 3 cut(s) 2496, 2887, 3866
BsmFI GGGAC 4 cut(s) 460, 490, 912, 2380
Bso31I GGTCTC 3 cut(s) 1127, 2443, 4312
Bsp120I GGGCCC 1 cut(s) 1747
Bsp1286I GDGCHC 9 cut(s) 45, 1165, 1387, 1751, 1926, 3814, 4296, 4592, 6066
Bsp1407I TGTACA 3 cut(s) 2309, 2819, 3075
Bsp19I CCATGG 1 cut(s) 3428
BspDI ATCGAT 1 cut(s) 3096
BspFNI CGCG 3 cut(s) 19, 5869, 6074
BspHI TCATGA 1 cut(s) 4831
BspLI GGNNCC 8 cut(s) 1748, 1749, 3033, 3251, 3434, 3630, 4156, 5142
BspMAI CTGCAG 2 cut(s) 4557, 5745
BspMI ACCTGC 2 cut(s) 785, 4560
BspQI GCTCTTC 2 cut(s) 1170, 4301
BspTNI GGTCTC 3 cut(s) 1127, 2443, 4312
BsrDI GCAATG 2 cut(s) 628, 2046
BsrFI RCCGGY 3 cut(s) 1583, 4788, 5355
BsrGI TGTACA 3 cut(s) 2309, 2819, 3075
BssAI RCCGGY 3 cut(s) 1583, 4788, 5355
BssNAI GTATAC 1 cut(s) 5471
BssNI GRCGYC 3 cut(s) 51, 2896, 3949
BssSI CACGAG 2 cut(s) 1697, 3084
BssT1I CCWWGG 7 cut(s) 2721, 3108, 3428, 4373, 4520, 5682, 5908
Bst1107I GTATAC 1 cut(s) 5471
Bst2BI CACGAG 2 cut(s) 1697, 3084
Bst6I CTCTTC 6 cut(s) 246, 1170, 1898, 3869, 4301, 5939
BstACI GRCGYC 3 cut(s) 51, 2896, 3949
BstAPI GCANNNNNTGC 7 cut(s) 1160, 1769, 2756, 4406, 4561, 4947, 5548
BstAUI TGTACA 3 cut(s) 2309, 2819, 3075
BstBAI YACGTR 1 cut(s) 938
BstDSI CCRYGG 3 cut(s) 17, 3428, 4150
BstFNI CGCG 3 cut(s) 19, 5869, 6074
BstH2I RGCGCY 3 cut(s) 504, 1050, 2275
BstHHI GCGC 7 cut(s) 454, 503, 1008, 1049, 1785, 2274, 4966
BstNSI RCATGY 6 cut(s) 391, 693, 1594, 2416, 5375, 5542
BstSFI CTRYAG 4 cut(s) 2541, 4236, 4553, 5741
BstSLI GKGCMC 2 cut(s) 1751, 1926
BstUI CGCG 3 cut(s) 19, 5869, 6074
BstV2I GAAGAC 4 cut(s) 105, 4551, 5109, 5136
BstXI CCANNNNNNTGG 1 cut(s) 1849
BstZ17I GTATAC 1 cut(s) 5471
Bsu15I ATCGAT 1 cut(s) 3096
Bsu36I CCTNAGG 1 cut(s) 5877
BsuI GTATCC 3 cut(s) 1301, 4343, 5962
BsuTUI ATCGAT 1 cut(s) 3096
BtgI CCRYGG 3 cut(s) 17, 3428, 4150
BtgZI GCGATG 1 cut(s) 1529
BtsI GCAGTG 3 cut(s) 222, 1681, 3755
BveI ACCTGC 2 cut(s) 785, 4560
CaiI CAGNNNCTG 6 cut(s) 980, 1397, 1867, 2756, 3449, 5740
CciI TCATGA 1 cut(s) 4831
CfoI GCGC 7 cut(s) 454, 503, 1008, 1049, 1785, 2274, 4966
Cfr10I RCCGGY 3 cut(s) 1583, 4788, 5355
Cfr13I GGNCC 9 cut(s) 1747, 1748, 3032, 3249, 3386, 4154, 4486, 5353, 5905
Cfr42I CCGCGG 1 cut(s) 20
ClaI ATCGAT 1 cut(s) 3096
CseI GACGC 2 cut(s) 2904, 3957
CspAI ACCGGT 1 cut(s) 5355
CspCI CAANNNNNGTGG 2 cut(s) 3527, 3562
DraI TTTAAA 5 cut(s) 1483, 2580, 3477, 4306, 5811
DriI GACNNNNNGTC 2 cut(s) 3953, 5449
EaeI YGGCCR 4 cut(s) 747, 4014, 4786, 4790
Eam1104I CTCTTC 6 cut(s) 246, 1170, 1898, 3869, 4301, 5939
Eam1105I GACNNNNNGTC 2 cut(s) 3953, 5449
EarI CTCTTC 6 cut(s) 246, 1170, 1898, 3869, 4301, 5939
Ecl136II GAGCTC 1 cut(s) 3812
Eco130I CCWWGG 7 cut(s) 2721, 3108, 3428, 4373, 4520, 5682, 5908
Eco147I AGGCCT 2 cut(s) 62, 4230
Eco24I GRGCYC 4 cut(s) 45, 1751, 3814, 4296
Eco31I GGTCTC 3 cut(s) 1127, 2443, 4312
Eco32I GATATC 2 cut(s) 4033, 4762
Eco47I GGWCC 5 cut(s) 3032, 4154, 4486, 5353, 5905
Eco53kI GAGCTC 1 cut(s) 3812
Eco57I CTGAAG 6 cut(s) 270, 1503, 4491, 5061, 5067, 5963
Eco81I CCTNAGG 1 cut(s) 5877
EcoICRI GAGCTC 1 cut(s) 3812
EcoO109I RGGNCCY 3 cut(s) 1747, 1748, 3032
EcoRI GAATTC 1 cut(s) 5775
EcoRV GATATC 2 cut(s) 4033, 4762
EcoT14I CCWWGG 7 cut(s) 2721, 3108, 3428, 4373, 4520, 5682, 5908
EcoT22I ATGCAT 4 cut(s) 691, 5117, 5544, 5612
EcoT38I GRGCYC 4 cut(s) 45, 1751, 3814, 4296
ErhI CCWWGG 7 cut(s) 2721, 3108, 3428, 4373, 4520, 5682, 5908
Esp3I CGTCTC 3 cut(s) 2496, 2887, 3866
FalI AAGNNNNNCTT 4 cut(s) 571, 603, 5370, 5402
FaqI GGGAC 4 cut(s) 460, 490, 912, 2380
FauI CCCGC 4 cut(s) 3150, 5504, 5762, 5874
FauNDI CATATG 1 cut(s) 3509
FbaI TGATCA 3 cut(s) 634, 4116, 4801
FblI GTMKAC 3 cut(s) 4462, 4999, 5470
FriOI GRGCYC 4 cut(s) 45, 1751, 3814, 4296
FseI GGCCGGCC 1 cut(s) 4792
GlaI GCGC 7 cut(s) 453, 502, 1007, 1048, 1784, 2273, 4965
GsaI CCCAGC 4 cut(s) 2241, 2448, 3877, 5156
GsuI CTGGAG 4 cut(s) 71, 750, 3411, 3987
HaeII RGCGCY 3 cut(s) 504, 1050, 2275
HapII CCGG 4 cut(s) 1584, 4789, 5356, 5717
HgaI GACGC 2 cut(s) 2904, 3957
HhaI GCGC 7 cut(s) 454, 503, 1008, 1049, 1785, 2274, 4966
Hin1I GRCGYC 3 cut(s) 51, 2896, 3949
Hin6I GCGC 7 cut(s) 452, 501, 1006, 1047, 1783, 2272, 4964
HinP1I GCGC 7 cut(s) 452, 501, 1006, 1047, 1783, 2272, 4964
HincII GTYRAC 2 cut(s) 2534, 4463
HindII GTYRAC 2 cut(s) 2534, 4463
HindIII AAGCTT 7 cut(s) 171, 661, 1703, 2622, 3139, 3308, 3638
HpaII CCGG 4 cut(s) 1584, 4789, 5356, 5717
Hpy166II GTNNAC 9 cut(s) 2534, 2683, 4154, 4463, 4609, 5000, 5347, 5471, 5862
Hpy8I GTNNAC 9 cut(s) 2534, 2683, 4154, 4463, 4609, 5000, 5347, 5471, 5862
Hpy99I CGWCG 4 cut(s) 56, 4464, 4886, 5233
HpyCH4IV ACGT 8 cut(s) 51, 937, 2489, 2761, 2876, 3541, 5228, 5436
HpySE526I ACGT 8 cut(s) 51, 937, 2489, 2761, 2876, 3541, 5228, 5436
Hsp92I GRCGYC 3 cut(s) 51, 2896, 3949
HspAI GCGC 7 cut(s) 452, 501, 1006, 1047, 1783, 2272, 4964
KroI GCCGGC 1 cut(s) 4788
KroNI GCCGGC 1 cut(s) 4790
Ksp22I TGATCA 3 cut(s) 634, 4116, 4801
KspI CCGCGG 1 cut(s) 20
LguI GCTCTTC 2 cut(s) 1170, 4301
MaeII ACGT 8 cut(s) 51, 937, 2489, 2761, 2876, 3541, 5228, 5436
MfeI CAATTG 1 cut(s) 2372
MhlI GDGCHC 9 cut(s) 45, 1165, 1387, 1751, 1926, 3814, 4296, 4592, 6066
MlsI TGGCCA 2 cut(s) 749, 4016
MluNI TGGCCA 2 cut(s) 749, 4016
MlyI GAGTC 6 cut(s) 140, 761, 1884, 2362, 3463, 5624
MmeI TCCRAC 5 cut(s) 114, 190, 1412, 2203, 3195
Mox20I TGGCCA 2 cut(s) 749, 4016
Mph1103I ATGCAT 4 cut(s) 691, 5117, 5544, 5612
MroNI GCCGGC 1 cut(s) 4788
MroXI GAANNNNTTC 4 cut(s) 255, 3055, 4875, 6081
MscI TGGCCA 2 cut(s) 749, 4016
MslI CAYNNNNRTG 7 cut(s) 1415, 1473, 2354, 2904, 4597, 5120, 5762
Msp20I TGGCCA 2 cut(s) 749, 4016
MspA1I CMGCKG 7 cut(s) 19, 73, 302, 4028, 4655, 5511, 5793
MspI CCGG 4 cut(s) 1584, 4789, 5356, 5717
MunI CAATTG 1 cut(s) 2372
MvnI CGCG 3 cut(s) 19, 5869, 6074
NaeI GCCGGC 1 cut(s) 4790
NciI CCSGG 1 cut(s) 5717
NcoI CCATGG 1 cut(s) 3428
NdeI CATATG 1 cut(s) 3509
NgoMIV GCCGGC 1 cut(s) 4788
NlaIV GGNNCC 8 cut(s) 1748, 1749, 3033, 3251, 3434, 3630, 4156, 5142
NmeAIII GCCGAG 1 cut(s) 3868
NmuCI GTSAC 5 cut(s) 797, 1525, 1676, 2605, 5437
NsiI ATGCAT 4 cut(s) 691, 5117, 5544, 5612
NspI RCATGY 6 cut(s) 391, 693, 1594, 2416, 5375, 5542
OliI CACNNNNGTG 1 cut(s) 2354
PaeI GCATGC 2 cut(s) 2416, 5542
PagI TCATGA 1 cut(s) 4831
PaqCI CACCTGC 1 cut(s) 785
PceI AGGCCT 2 cut(s) 62, 4230
PciI ACATGT 1 cut(s) 5371
PciSI GCTCTTC 2 cut(s) 1170, 4301
PdiI GCCGGC 1 cut(s) 4790
PdmI GAANNNNTTC 4 cut(s) 255, 3055, 4875, 6081
PflMI CCANNNNNTGG 1 cut(s) 2390
PinAI ACCGGT 1 cut(s) 5355
PleI GAGTC 6 cut(s) 139, 761, 1884, 2362, 3462, 5624
PpsI GAGTC 6 cut(s) 139, 761, 1884, 2362, 3462, 5624
Ppu21I YACGTR 1 cut(s) 938
PpuMI RGGWCCY 1 cut(s) 3032
PscI ACATGT 1 cut(s) 5371
PshBI ATTAAT 2 cut(s) 1982, 4691
PsiI TTATAA 3 cut(s) 1356, 2483, 2657
Psp124BI GAGCTC 1 cut(s) 3814
Psp1406I AACGTT 1 cut(s) 3541
Psp5II RGGWCCY 1 cut(s) 3032
PspFI CCCAGC 4 cut(s) 2237, 2444, 3873, 5152
PspN4I GGNNCC 8 cut(s) 1748, 1749, 3033, 3251, 3434, 3630, 4156, 5142
PspOMI GGGCCC 1 cut(s) 1747
PspPI GGNCC 9 cut(s) 1747, 1748, 3032, 3249, 3386, 4154, 4486, 5353, 5905
PspPPI RGGWCCY 1 cut(s) 3032
PstI CTGCAG 2 cut(s) 4557, 5745
PstNI CAGNNNCTG 6 cut(s) 980, 1397, 1867, 2756, 3449, 5740
PvuII CAGCTG 1 cut(s) 4655
RigI GGCCGGCC 1 cut(s) 4792
RseI CAYNNNNRTG 7 cut(s) 1415, 1473, 2354, 2904, 4597, 5120, 5762
SacI GAGCTC 1 cut(s) 3814
SacII CCGCGG 1 cut(s) 20
SalI GTCGAC 1 cut(s) 4461
SapI GCTCTTC 2 cut(s) 1170, 4301
Sau96I GGNCC 9 cut(s) 1747, 1748, 3032, 3249, 3386, 4154, 4486, 5353, 5905
ScaI AGTACT 1 cut(s) 715
SchI GAGTC 6 cut(s) 140, 761, 1884, 2362, 3463, 5624
SduI GDGCHC 9 cut(s) 45, 1165, 1387, 1751, 1926, 3814, 4296, 4592, 6066
SfcI CTRYAG 4 cut(s) 2541, 4236, 4553, 5741
Sfr303I CCGCGG 1 cut(s) 20
SgrAI CRCCGGYG 1 cut(s) 1583
SgrBI CCGCGG 1 cut(s) 20
SinI GGWCC 5 cut(s) 3032, 4154, 4486, 5353, 5905
SmiI ATTTAAAT 1 cut(s) 2580
SmiMI CAYNNNNRTG 7 cut(s) 1415, 1473, 2354, 2904, 4597, 5120, 5762
SmlI CTYRAG 6 cut(s) 670, 1170, 1290, 2863, 2987, 4163
SmoI CTYRAG 6 cut(s) 670, 1170, 1290, 2863, 2987, 4163
SpeI ACTAGT 2 cut(s) 1375, 1765
SphI GCATGC 2 cut(s) 2416, 5542
SseBI AGGCCT 2 cut(s) 62, 4230
SspI AATATT 6 cut(s) 2956, 3413, 3660, 4446, 4715, 5652
SstI GAGCTC 1 cut(s) 3814
StuI AGGCCT 2 cut(s) 62, 4230
StyI CCWWGG 7 cut(s) 2721, 3108, 3428, 4373, 4520, 5682, 5908
SwaI ATTTAAAT 1 cut(s) 2580
TaiI ACGT 8 cut(s) 54, 940, 2492, 2764, 2879, 3544, 5231, 5439
TatI WGTACW 5 cut(s) 713, 1087, 2309, 2819, 3075
TauI GCSGC 4 cut(s) 22, 96, 190, 5293
TseFI GTSAC 5 cut(s) 797, 1525, 1676, 2605, 5437
Tsp45I GTSAC 5 cut(s) 797, 1525, 1676, 2605, 5437
TspGWI ACGGA 1 cut(s) 3680
Van91I CCANNNNNTGG 1 cut(s) 2390
VpaK11BI GGWCC 5 cut(s) 3032, 4154, 4486, 5353, 5905
VspI ATTAAT 2 cut(s) 1982, 4691
XapI RAATTY 9 cut(s) 494, 1072, 1283, 1478, 2213, 2945, 4128, 5056, 5775
XceI RCATGY 6 cut(s) 391, 693, 1594, 2416, 5375, 5542
XcmI CCANNNNNNNNNTGG 3 cut(s) 419, 3507, 4352
XmiI GTMKAC 3 cut(s) 4462, 4999, 5470
XmnI GAANNNNTTC 4 cut(s) 255, 3055, 4875, 6081
ZraI GACGTC 1 cut(s) 52
ZrmI AGTACT 1 cut(s) 715
Zsp2I ATGCAT 4 cut(s) 691, 5117, 5544, 5612
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.