Rh7BG054200

Activating signal cointegrator 1 complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0008552
Physical Location & Seq
Reverse (-)
3610645 .. 3629557
18913 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG054200.1

Sequence Viewer

Length: 6246 bp
ATGCTGGTTCAGCTTCCGCGATTGACTAGCTCTCTGAGGGAGCCCTTCGACGTCGACCAGGCCTATTTGCAGCGCAAGCTCATCCTTCAAAACCACACCAAGCCTGGCCAATCATCGAGCTCAGTTGATGAGTCGGAGCTTGCGAGAAAGATAGTTCATAGATGGGAGGAAGCTTCGTATGAGCTGCGGCAAGCTTACAAGCAGTTTATTGGAGCTGTGGTGGAGTTGATAGATGGAGAAGTTCAATCCGAGGAGTTTCGTGAGGTGGCACTCGTTGTTTACCGGCTTTTTGGCAGACCGGAGGAGGAAGGCAGTGTTGAAACAAATTTTGCCGGAAAAAAGCAGGAAGTGCAAATGCTTTTGGGGCACACAGTTTCAGATGCTAACATGCGGAAAGTTGCGTCTCTAGCTCAGAGACTTTGTGGGATGCAGTCTAGTGATCATGGGATTACACTTGTTTCAGAAAGGCCTGTAAATGGATCTCATGACGATGCAGAATTTGGTGCCGACCTTGTTTTTCATCCTCCGGCTCGGTTTTTGGTAGATGTTTCTTTGGATGATGCGGAGTCATTTTGTGAGGAAACCGCTGGACCTTCCTCTTATTATGAAGGATCATATGGTCACAGTGGCTTGAGTGGTGGTCATTCTGCTACTGATGGGCGCAGCATCGATCTGACTTGGCTACAAGATGCATGTGCTCAGATAACAAAAAGTACTACACAGCTTTCTCGAGATGAACTGGCTATGGCCATTTGTCGGGTGCTTGATTCTGACAAACCTGGTGACGAGATAGCAGGTGACTTGTTGGACCTTGTGGGGGATAGTGCATTTGAAACTGTTCAAGACCTCATATCACATCGAAAGGAACTTGTTGATGCTATCCATCATGGGTTACTAGTAATGAAATCTGACAAATCGTCTTTGACCTCTCAACCTCGCATGCCTAGTTATGGCACACAGGTTACTGTTCAAACAGAATCTGAACGGCAGATTGATAAGCTTCGGCGCAAAGAGGAAAAGCGAAATAGACGAGGAACAGAATATGGCACTGATAGTGATCTGGCTGCTGTAAATTTTTCTTCTCTACTACAAGCAAGTGAGAAAAAAAATATTTTTGATGATCTATCGGGCCTTGGTGAAGGGATAGCGGTTAATGCTCTTCCGCAAGGAACTCAGAGGAAGCACCACAAGGGGTATGAGGAGGTCCTAATTCCTCCTACACCAGGAGCACAAATGAAACCTGGAGAAAAGCTGATTGAGATCTCGGAGTTAGATGAGTTTGCTCAAGCTGCTTTTCGTGGATACAAATCTTTGAACCGCATTCAGAGCCGCATATATCATACTGTGTACTACACAAATGAGAATATACTAGTTTGTGCTCCAACAGGAGCTGGCAAAACAAACATAGCTATGATTTCCATTCTACATGAGATTGGACAGCACTTCAAAGATGGTTACCTGCACAAAGACGAGTTCAAAATAGTTTATGTTGCACCTATGAAGGCGTTAGCTGCAGAAGTCACATCAACTTTTAGCCATCGGTTATCTCCATTAAATATGACTGTGAGAGAACTTACTGGAGACATGCAACTTTCTAAGAATGAACTTGAAGAAACTCAGATGATAGTTACAACACCTGAGAAATGGGATGTCATTACTCGCAAGAGCAGCGACATGTCACTTTCAATGCTGGTGAAGCTCCTAATAATTGATGAAGTTCATCTTTTGAATGATGATAGAGGCCCGGTAATTGAGGCACTAGTTGCTCGGACTTTACGCCAGGTGGAGTCTTCACAGTCAATGATACGCATTGTGGGACTTTCCGCCACTCTACCCAACTATTTGGAGGTCGCACAGTTTCTGAGAGTGAATCCAGAGGCAGGACTCTTCTACTTTGATTCTAGCTATCGTCCAGTGCCCCTTGCACAACAGTATATTGGTATTAGTGAGACAAACTTTTCAGCCAAAACTGAATTGTTGAATGAGATATGCTACAAGAAGGTTGTGGAGTCACTAAGGCAAGGTCATCAGGCAATGGTATTTGTCCATTCTCGAAAGGACACAGCAAAAACGGCTCAGAAGCTGGTTGAACTTGCTCGCAAATTTGAAGGACTGGAGCTCTTTAAGAATGATCAGCACCCACAATTTTCATTGATCCAGAGGGAAGTTGTAAAATCCAGAAACAAAGATCTTGTTGAACTTTTTGAATTCGGAATTGGAATTCATAATGCTGGGATGTTACGTTCTGATAGAGGTCTGACTGAGCGCCTTTTCTCTGATGGACTATTGAAGGTACTTGTTTGTACAGCAACTTTGGCGTGGGGCGTAAATCTACCAGCTCACACTGTTGTGATAAAGGGGACCCAATTATATGATCCAAAGGCTGGTGGGTGGCGAGACCTGGGAATGCTTGATGTTATGCAGATATTTGGACGGGCTGGGAGACCTCAATTCGATAACAGTGGTGAAGGTATTATAATCACGTCTCATGACAAGCTGGCCTACTATCTGCGGTTGCTGACGAGTCAACTTCCTATTGAAAGTCAGTTCATTAGTTCCTTGAAGGACAATTTAAATGCAGAGGTGGCACTTGGAACTGTCACCAATGTCAAGGAAGCTTGTGCATGGCTTGGATATACATACCTTTTTATAAGGATGAGGCTCAACCCTTTGGCATATGGTATCGCGTGGGAGGAGGTTATGGCAGATCCTTCCTTGAGTTTAAAGCAGAGATCTCTCGTAGCAGATGCTGCGCGTGCCCTTGACAAAGCAAAAATGATGCGGTTTGACGAGAAAAGTGGCAATTTTTACTGTACAGAGCTTGGTCGCATAGCAAGCCACTTTTATATTCAGTACTCTAGTGTTGAAACCTACAATGAAATGTTGAAACGTCACATGAATGAGACTGAGGTAATTGACATGGTAGCCCACTCCTCGGAATTTGAAAATATTGTTATTCGAGAAGAGGAACAAAATGAGCTTGAGATGTTGGTGCGAAAATTGTGTCCAGTAGAAGTTAGGGGTGGTCCTTCAAATAAACATGGAAAGATTTCCGTTCTCATTCAGGTGTACATATCTAGAGGCTCAATTGATACCTTTTCTTTGGTTTCTGATGCTCAATATATAAGTGCAAGCTTGGCACGTATTATTCGGGCCTTATTTGAGATTTGTTTGCGAAAAGGCTGGAGTGAGATGTCATTGTTCATGTTGGAATATTGCAAGGCGGTGGACCGCCAAGTTTGGCCCCATCAACATCCTTTCAGACAATTTGACAGGGAGATTTCAGCACAAATACTGCGAAATCTTGAGGAACGAGGTGCTGATCTAGATCGCCTATATGATATGGAGGAAAAAGAGATTGGGAAACTGGTCAATTATGGACCTGGAGGAAGGTTGGTTAAGCAATATTTGGGATATTTTCCATGGATCCAGTTAGAAGCAACTGTAAGTCCAATTACCAGAACTGTTTTAAAGGTGGATTTGCATATTAAACCAGACTTTATATGGAAAGATCGTTTTCACGGCACCGCCCAGCGTTGGTGGATTCTAGTTGAGGATTCTGAGAATGATCATATCTACCACTCGGAGCTATTCACTCTAACAAAGCGGATGGCTAAAGGGGAACCTCAGAAGCTCTCGTTCACTGTGCCAATATTTGAGCCACATCCGCCACAGTATTATATTCGTGCTGTTTCTGATTCTTGGTTGCATGCAGAGGCATTCTACACCATTTCTTTCCATAATCTAACACTACCAGAGGCCCATACTTCACATACAGAACTGCTAGATTTAAAGCCTCTCCCTGTGACATCTCTTGGAAACACTGCTTATGAAGCATTGTACAAATTCTCGCACTTTAATCCAATACAAACACAGACTTTCCATGTGCTGTATCACACAGACAATAATGTATTACTGGGAGCTCCTACTGGAAGTGGAAAAACTATTTCTGCTGAGCTTGCTATGCTTCGTCTCTTCAACACCCAGCCTGATATGAAGGTCATTTACATAGCACCTTTGAAGGCTATTGTTCGAGAAAGAATGAACGATTGGAGGAAGCGCCTTGTCTCTCAGCTAGGGAAGAAAATGGTTGAAATGACAGGAGATTATACTCCAGATTTGATGGCCATCTTGTCAGCAGATATCATAATATCAACTCCTGAAAAATGGGATGGTATCAGTCGTAATTGGCATAGCCGAACCTATGTTAAAAAGGTTGGGCTTATGATTTTGGATGAGATTCACTTGTTGGGAGCTGACCGTGGGCCTATCCTCGAGGTTATTGTGTCAAGGATGAGATACATATCCTCACAGACAGAGCGTGAAGTACGGTTTGTAGGCCTTTCTACAGCTCTGGCAAATGCAGGAGATTTAGCTGATTGGTTGGGTGTTGGAGAAACTGGACTCTTCAATTTCAAACCAAGTGTGAGACCTGTACCTCTTGAAGTTCATATCCAGGGATATCCAGGAAAGTTCTATTGTCCAAGGATGAATAGCATGAACAAACCATCATATGCTGCTATATGCACCCATTCACCTACAAAACCGGTTCTAATTTTTGTTTCCTCACGTCGCCAGACAAGGCTTACTGCATTGGACCTTATTCAGTATGCAGCTTCAGATGAGCATCCAAGGCAGTTTCTTAGTATACCAGAAGAAGAACTGCAGATGGTTCTTTATCAGGTTGCTGATAGTAACCTGAGGCACACCTTGCAATTTGGCATTGGATTACACCATGCTGGTCTGAATGACAAAGATAGATCCCTGGTTGAGGAACTTTTTGCAAACAACAAGATTCAGGTATTGGTTTGTACTAGCACATTGGCATGGGGTGTAAACCTTCCAGCGCATCTGGTTATTATTAAGGGAACAGAATTTTATGATGGGAAAACAAAAAGATATGTCGACTTCCCAATCACAGATATCTTGCAAATGATGGGTCGTGCAGGTCGCCCACAATTTGATCAACACGGGAAAGCAGTCATTCTTGTTCATGAACCCAAAAAGAGCTTCTATAAAAAGTTTTTATATGAACCATTTCCTGTCGAGAGTAGTCTGAGGGAACAGCTGCATAATCACATTAATGCGGAGATAGTTTCTGGCACAATTTGCCATAAAGAGGATGCATTGCATTATCTTACGTGGACATATTTATTCCGCAGACTGATGTTTAATCCGGCTTACTATGGCTTGGACAATACTGACGCCGAAGTTCTCAGTTCTTATTTGTCTAGGTTAGTGCAGAACACATTGGAGGATCTGGAAGACAGTGGATGCATCAAGATGAGTGAAGACAGTGTGGAGCCAATGATGCTGGGTTCGATAGCATCCCAGTATTACCTTAGCTACTTGACTGTATCGATGTTTGGTTCAAACATTGGCTCAGATACCTCTCTTGAAGTATTCTTGCATATCCTATCTGCTGCTTCTGAATATGATGAGCTTCCTGTTCGCCATAACGAGGAAAATTACAATGAGGCATTGTCCGAGAGAGTTCGATATAAAGTGGATAAGGATCGCCTAGATGATCCACATGTCAAAGCAAATCTGCTATTTCAGGCACATTTCTCGCAGTTAGAATTACCAATTACTGACTATGTCACAGACTTGAAGTCAGTTTTGGATCAAAGTATACGGATTATCCAGGCCATGATAGATATATGTGCCAACAGTGGGTGGCTTTCAAGTTCAGTTACTTGCATGCATCTTTTGCAGATGGTCATGCAGGGCTTATGGTTTGATGAGGATTCTTCCTTATGGATGTTGCCTTGCATGAATGTTGAACTTGCGGAATCACTCGGCAAACGAGGAATCTTCCATATACAGCAGCTATTGGAACTTCCCAAGGCAACTTTGCAGAACATGATTGGAAACTTCCCTGCTTCAAAATTCTTTCAGGATCTGCAGCTCTTTCCACGCATCGAAGTCAAGTTAAAAATTCTACAGAAAGATGGTGGAAAATCCTGCTCTTTAAACATCAGATTGGTGAAGACCAATTTCAGGAAATATAAATCAAGAGCATTCACTCCACGTTTTCCTAAGGTGAAAAATGAAGCATGGTGGTTGGTCCTTGGGAACACCTCTACGTTGGAACTATATGCTCTGAAGAGAGTATCTTTCTCTGATCACTTGGTTACTAACATGGAGTTACCATCCGATTCAACCACTCTCCAGGGGATGAAACTGATTTTAATATCAGATTGTTATCTGGGGTTCGAACAAGAGCATTCAATTTCAGAACTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DEAD Helicase_C Sec63

Protein Analysis

2081

Amino Acids

235.97

Weight (kDa)

5.99

Isoelectric Point (pI)

41.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DExH14_plug PF24557 45 - 144 9.9e-44 DExH14 plug domain
DEAD PF00270 439 - 613 1.5e-30 DEAD/DEAH box helicase
ResIII PF04851 450 - 612 3.2e-12 Type III restriction enzyme, res subunit
Helicase_C PF00271 730 - 816 3.6e-06 Helicase conserved C-terminal domain
WHD_SNRNP200 PF23445 847 - 916 8.6e-15 Winged Helix-turn-helix domain
Sec63 PF02889 938 - 1245 2.5e-88 Sec63 Brl domain
DEAD PF00270 1287 - 1453 2.6e-28 DEAD/DEAH box helicase
ResIII PF04851 1295 - 1420 6.6e-11 Type III restriction enzyme, res subunit
Helicase_C PF00271 1565 - 1652 2.5e-09 Helicase conserved C-terminal domain
WHD_SNRNP200 PF23445 1683 - 1763 8.1e-32 Winged Helix-turn-helix domain
Sec63 PF02889 1769 - 2079 4.9e-65 Sec63 Brl domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 2477, 2651
AarI CACCTGC 1 cut(s) 785
AatII GACGTC 1 cut(s) 54
AbsI CCTCGAGG 1 cut(s) 4274
Acc36I ACCTGC 3 cut(s) 785, 1467, 4937
AccB1I GGYRCC 2 cut(s) 503, 3524
AccB7I CCANNNNNTGG 1 cut(s) 2384
AccI GTMKAC 4 cut(s) 54, 4648, 4905, 5632
AccII CGCG 3 cut(s) 19, 2687, 2755
AcoI YGGCCR 3 cut(s) 106, 747, 4125
AcuI CTGAAG 2 cut(s) 4602, 6125
AcyI GRCGYC 2 cut(s) 51, 5205
AflIII ACRYGT 2 cut(s) 1674, 5533
AgeI ACCGGT 1 cut(s) 4546
AhdI GACNNNNNGTC 2 cut(s) 916, 5611
AhlI ACTAGT 3 cut(s) 895, 1369, 1759
AjiI CACGTC 2 cut(s) 2484, 4571
AleI CACNNNNGTG 1 cut(s) 2348
AloI GAACNNNNNNTCC 2 cut(s) 2227, 2259
Alw21I GWGCWC 6 cut(s) 122, 700, 1231, 1381, 2121, 3925
AlwNI CAGNNNCTG 6 cut(s) 980, 1391, 1861, 2083, 2750, 5902
Ama87I CYCGRG 2 cut(s) 729, 4274
ArsI GACNNNNNNTTYG 4 cut(s) 2989, 3021, 4924, 4956
AseI ATTAAT 1 cut(s) 5082
AsiGI ACCGGT 1 cut(s) 4546
Asp700I GAANNNNTTC 3 cut(s) 837, 3049, 5037
AspLEI GCGC 7 cut(s) 75, 663, 1008, 2268, 2755, 4062, 4849
AsuC2I CCSGG 1 cut(s) 1745
AsuHPI GGTGA 9 cut(s) 794, 809, 1148, 1705, 2477, 2593, 4527, 5998, 6055
AsuII TTCGAA 1 cut(s) 6216
AvaI CYCGRG 2 cut(s) 729, 4274
AvaII GGWCC 9 cut(s) 590, 808, 1204, 2361, 3026, 3229, 3380, 4597, 6067
AxyI CCTNAGG 2 cut(s) 4700, 6039
BaeGI GKGCMC 3 cut(s) 369, 1920, 2761
BalI TGGCCA 3 cut(s) 108, 749, 4127
BamHI GGATCC 1 cut(s) 3426
BanI GGYRCC 2 cut(s) 503, 3524
BanII GRGCYC 4 cut(s) 45, 122, 2121, 3925
BarI GAAGNNNNNNTAC 2 cut(s) 3989, 4021
BbsI GAAGAC 4 cut(s) 1782, 5271, 5298, 5996
Bbv12I GWGCWC 6 cut(s) 122, 700, 1231, 1381, 2121, 3925
BceAI ACGGC 3 cut(s) 1001, 2088, 3538
BcgI CGANNNNNNTGC 2 cut(s) 5550, 5584
BciVI GTATCC 1 cut(s) 1295
BclI TGATCA 5 cut(s) 439, 2131, 3568, 4963, 6124
BcnI CCSGG 1 cut(s) 1745
BcuI ACTAGT 3 cut(s) 895, 1369, 1759
BfmI CTRYAG 5 cut(s) 1512, 4347, 4664, 5903, 5942
BfoI RGCGCY 2 cut(s) 2269, 4063
BfuAI ACCTGC 3 cut(s) 785, 1467, 4937
BfuI GTATCC 1 cut(s) 1295
BglII AGATCT 3 cut(s) 1260, 2188, 2732
BlpI GCTNAGC 1 cut(s) 3954
BmcAI AGTACT 2 cut(s) 715, 2855
Bme18I GGWCC 9 cut(s) 590, 808, 1204, 2361, 3026, 3229, 3380, 4597, 6067
BmeRI GACNNNNNGTC 2 cut(s) 916, 5611
BmeT110I CYCGRG 2 cut(s) 729, 4274
BmgBI CACGTC 2 cut(s) 2484, 4571
BmiI GGNNCC 9 cut(s) 42, 505, 2362, 2363, 3245, 3428, 3526, 3624, 5304
BmrI ACTGGG 2 cut(s) 3926, 5326
BmuI ACTGGG 2 cut(s) 3926, 5326
BpiI GAAGAC 4 cut(s) 1782, 5271, 5298, 5996
BpmI CTGGAG 7 cut(s) 1263, 1599, 2135, 3205, 3405, 4098, 6155
Bpu10I CCTNAGC 1 cut(s) 5342
Bpu1102I GCTNAGC 1 cut(s) 3954
Bpu14I TTCGAA 1 cut(s) 6216
BpuEI CTTGAG 5 cut(s) 652, 1269, 2737, 3002, 3326
BpuMI CCSGG 1 cut(s) 1745
Bsa29I ATCGAT 2 cut(s) 669, 5360
BsaAI YACGTR 2 cut(s) 3143, 5142
BsaBI GATNNNNATC 9 cut(s) 1056, 1259, 1306, 3327, 4127, 4303, 4626, 5514, 6204
BsaHI GRCGYC 2 cut(s) 51, 5205
BsaI GGTCTC 3 cut(s) 2391, 2437, 4423
BsaWI WCCGGW 2 cut(s) 298, 4546
BsaXI ACNNNNNCTCC 2 cut(s) 204, 234
Bse118I RCCGGY 2 cut(s) 282, 4546
Bse21I CCTNAGG 2 cut(s) 4700, 6039
Bse3DI GCAATG 2 cut(s) 2040, 5126
Bse8I GATNNNNATC 9 cut(s) 1056, 1259, 1306, 3327, 4127, 4303, 4626, 5514, 6204
BseCI ATCGAT 2 cut(s) 669, 5360
BseJI GATNNNNATC 9 cut(s) 1056, 1259, 1306, 3327, 4127, 4303, 4626, 5514, 6204
BseMI GCAATG 2 cut(s) 2040, 5126
BseRI GAGGAG 5 cut(s) 266, 317, 1214, 2708, 2923
BseSI GKGCMC 3 cut(s) 369, 1920, 2761
BseYI CCCAGC 5 cut(s) 2231, 2438, 3531, 3984, 5314
BsgI GTGCAG 3 cut(s) 1445, 4965, 5261
Bsh1236I CGCG 3 cut(s) 19, 2687, 2755
BshNI GGYRCC 2 cut(s) 503, 3524
BshTI ACCGGT 1 cut(s) 4546
BshVI ATCGAT 2 cut(s) 669, 5360
BsiHKAI GWGCWC 6 cut(s) 122, 700, 1231, 1381, 2121, 3925
BsiHKCI CYCGRG 2 cut(s) 729, 4274
BsiSI CCGG 7 cut(s) 283, 299, 333, 527, 1745, 4547, 5177
BslFI GGGAC 2 cut(s) 1830, 2374
BsmBI CGTCTC 3 cut(s) 408, 2490, 3977
BsmFI GGGAC 2 cut(s) 1830, 2374
BsmI GAATGC 5 cut(s) 1320, 2412, 3719, 6020, 6226
Bso31I GGTCTC 3 cut(s) 2391, 2437, 4423
BsoBI CYCGRG 2 cut(s) 729, 4274
Bsp119I TTCGAA 1 cut(s) 6216
Bsp1407I TGTACA 4 cut(s) 2303, 2813, 3069, 3840
Bsp1720I GCTNAGC 1 cut(s) 3954
Bsp19I CCATGG 1 cut(s) 3422
BspDI ATCGAT 2 cut(s) 669, 5360
BspFNI CGCG 3 cut(s) 19, 2687, 2755
BspHI TCATGA 3 cut(s) 484, 2488, 4993
BspLI GGNNCC 9 cut(s) 42, 505, 2362, 2363, 3245, 3428, 3526, 3624, 5304
BspMAI CTGCAG 3 cut(s) 1516, 4668, 5907
BspMI ACCTGC 3 cut(s) 785, 1467, 4937
BspQI GCTCTTC 1 cut(s) 1164
BspT104I TTCGAA 1 cut(s) 6216
BspT107I GGYRCC 2 cut(s) 503, 3524
BspTNI GGTCTC 3 cut(s) 2391, 2437, 4423
BsrDI GCAATG 2 cut(s) 2040, 5126
BsrFI RCCGGY 2 cut(s) 282, 4546
BsrGI TGTACA 4 cut(s) 2303, 2813, 3069, 3840
BssAI RCCGGY 2 cut(s) 282, 4546
BssNAI GTATAC 2 cut(s) 4649, 5633
BssNI GRCGYC 2 cut(s) 51, 5205
BssT1I CCWWGG 6 cut(s) 1132, 3422, 4484, 4631, 5844, 6070
Bst1107I GTATAC 2 cut(s) 4649, 5633
Bst6I CTCTTC 6 cut(s) 1164, 1892, 2958, 3980, 4412, 6101
BstACI GRCGYC 2 cut(s) 51, 5205
BstAPI GCANNNNNTGC 6 cut(s) 349, 1763, 2750, 4711, 5109, 5710
BstAUI TGTACA 4 cut(s) 2303, 2813, 3069, 3840
BstBAI YACGTR 2 cut(s) 3143, 5142
BstBI TTCGAA 1 cut(s) 6216
BstDSI CCRYGG 2 cut(s) 3422, 4261
BstEII GGTNACC 1 cut(s) 1454
BstENI CCTNNNNNAGG 2 cut(s) 1221, 4769
BstFNI CGCG 3 cut(s) 19, 2687, 2755
BstH2I RGCGCY 2 cut(s) 2269, 4063
BstHHI GCGC 7 cut(s) 75, 663, 1008, 2268, 2755, 4062, 4849
BstNSI RCATGY 8 cut(s) 391, 696, 943, 1588, 1678, 3713, 5537, 5704
BstPI GGTNACC 1 cut(s) 1454
BstSFI CTRYAG 5 cut(s) 1512, 4347, 4664, 5903, 5942
BstSLI GKGCMC 3 cut(s) 369, 1920, 2761
BstUI CGCG 3 cut(s) 19, 2687, 2755
BstV2I GAAGAC 4 cut(s) 1782, 5271, 5298, 5996
BstX2I RGATCY 9 cut(s) 479, 1260, 2188, 2707, 2732, 3426, 4760, 5257, 5899
BstXI CCANNNNNNTGG 1 cut(s) 1843
BstYI RGATCY 9 cut(s) 479, 1260, 2188, 2707, 2732, 3426, 4760, 5257, 5899
BstZ17I GTATAC 2 cut(s) 4649, 5633
Bsu15I ATCGAT 2 cut(s) 669, 5360
Bsu36I CCTNAGG 2 cut(s) 4700, 6039
BsuI GTATCC 1 cut(s) 1295
BsuTUI ATCGAT 2 cut(s) 669, 5360
BtgI CCRYGG 2 cut(s) 3422, 4261
BtrI CACGTC 2 cut(s) 2484, 4571
BtsI GCAGTG 2 cut(s) 319, 3822
BveI ACCTGC 3 cut(s) 785, 1467, 4937
CaiI CAGNNNCTG 6 cut(s) 980, 1391, 1861, 2083, 2750, 5902
CciI TCATGA 3 cut(s) 484, 2488, 4993
CfoI GCGC 7 cut(s) 75, 663, 1008, 2268, 2755, 4062, 4849
Cfr10I RCCGGY 2 cut(s) 282, 4546
ClaI ATCGAT 2 cut(s) 669, 5360
CseI GACGC 2 cut(s) 390, 5213
CsiI ACCWGGT 1 cut(s) 778
CspAI ACCGGT 1 cut(s) 4546
DraI TTTAAA 5 cut(s) 2574, 2724, 3471, 3792, 5973
DriI GACNNNNNGTC 2 cut(s) 916, 5611
EaeI YGGCCR 3 cut(s) 106, 747, 4125
Eam1104I CTCTTC 6 cut(s) 1164, 1892, 2958, 3980, 4412, 6101
Eam1105I GACNNNNNGTC 2 cut(s) 916, 5611
EarI CTCTTC 6 cut(s) 1164, 1892, 2958, 3980, 4412, 6101
EciI GGCGGA 2 cut(s) 1813, 3657
Ecl136II GAGCTC 3 cut(s) 120, 2119, 3923
Eco130I CCWWGG 6 cut(s) 1132, 3422, 4484, 4631, 5844, 6070
Eco147I AGGCCT 3 cut(s) 62, 469, 4341
Eco24I GRGCYC 4 cut(s) 45, 122, 2121, 3925
Eco31I GGTCTC 3 cut(s) 2391, 2437, 4423
Eco32I GATATC 3 cut(s) 4144, 4463, 4924
Eco47I GGWCC 9 cut(s) 590, 808, 1204, 2361, 3026, 3229, 3380, 4597, 6067
Eco53kI GAGCTC 3 cut(s) 120, 2119, 3923
Eco57I CTGAAG 2 cut(s) 4602, 6125
Eco81I CCTNAGG 2 cut(s) 4700, 6039
Eco88I CYCGRG 2 cut(s) 729, 4274
Eco91I GGTNACC 1 cut(s) 1454
EcoICRI GAGCTC 3 cut(s) 120, 2119, 3923
EcoNI CCTNNNNNAGG 2 cut(s) 1221, 4769
EcoO109I RGGNCCY 2 cut(s) 1204, 2361
EcoO65I GGTNACC 1 cut(s) 1454
EcoRI GAATTC 2 cut(s) 2207, 2220
EcoRV GATATC 3 cut(s) 4144, 4463, 4924
EcoT14I CCWWGG 6 cut(s) 1132, 3422, 4484, 4631, 5844, 6070
EcoT22I ATGCAT 4 cut(s) 694, 5128, 5279, 5706
EcoT38I GRGCYC 4 cut(s) 45, 122, 2121, 3925
ErhI CCWWGG 6 cut(s) 1132, 3422, 4484, 4631, 5844, 6070
Esp3I CGTCTC 3 cut(s) 408, 2490, 3977
FalI AAGNNNNNCTT 2 cut(s) 1707, 1739
FaqI GGGAC 2 cut(s) 1830, 2374
FauNDI CATATG 3 cut(s) 616, 2677, 4513
FbaI TGATCA 5 cut(s) 439, 2131, 3568, 4963, 6124
FblI GTMKAC 4 cut(s) 54, 4648, 4905, 5632
FriOI GRGCYC 4 cut(s) 45, 122, 2121, 3925
GlaI GCGC 7 cut(s) 74, 662, 1007, 2267, 2754, 4061, 4848
GsaI CCCAGC 5 cut(s) 2235, 2442, 3535, 3988, 5318
GsuI CTGGAG 7 cut(s) 1263, 1599, 2135, 3205, 3405, 4098, 6155
HaeII RGCGCY 2 cut(s) 2269, 4063
HapII CCGG 7 cut(s) 283, 299, 333, 527, 1745, 4547, 5177
HgaI GACGC 2 cut(s) 390, 5213
HhaI GCGC 7 cut(s) 75, 663, 1008, 2268, 2755, 4062, 4849
Hin1I GRCGYC 2 cut(s) 51, 5205
Hin6I GCGC 7 cut(s) 73, 661, 1006, 2266, 2753, 4060, 4847
HinP1I GCGC 7 cut(s) 73, 661, 1006, 2266, 2753, 4060, 4847
HincII GTYRAC 3 cut(s) 55, 2528, 4906
HindII GTYRAC 3 cut(s) 55, 2528, 4906
HindIII AAGCTT 5 cut(s) 171, 192, 998, 2616, 3133
HpaII CCGG 7 cut(s) 283, 299, 333, 527, 1745, 4547, 5177
HphI GGTGA 9 cut(s) 794, 809, 1148, 1705, 2477, 2593, 4527, 5998, 6055
Hpy99I CGWCG 3 cut(s) 53, 56, 4575
HpyCH4IV ACGT 9 cut(s) 51, 2242, 2483, 2890, 3142, 4570, 5141, 6031, 6086
HpySE526I ACGT 9 cut(s) 51, 2242, 2483, 2890, 3142, 4570, 5141, 6031, 6086
Hsp92I GRCGYC 2 cut(s) 51, 5205
HspAI GCGC 7 cut(s) 73, 661, 1006, 2266, 2753, 4060, 4847
KflI GGGWCCC 1 cut(s) 2361
Ksp22I TGATCA 5 cut(s) 439, 2131, 3568, 4963, 6124
LguI GCTCTTC 1 cut(s) 1164
MabI ACCWGGT 1 cut(s) 778
MaeII ACGT 9 cut(s) 51, 2242, 2483, 2890, 3142, 4570, 5141, 6031, 6086
MfeI CAATTG 1 cut(s) 3087
MflI RGATCY 9 cut(s) 479, 1260, 2188, 2707, 2732, 3426, 4760, 5257, 5899
MlsI TGGCCA 3 cut(s) 108, 749, 4127
MluNI TGGCCA 3 cut(s) 108, 749, 4127
MlyI GAGTC 7 cut(s) 140, 575, 1796, 1878, 2018, 2533, 4398
MmeI TCCRAC 6 cut(s) 114, 786, 1406, 3189, 4372, 6069
Mox20I TGGCCA 3 cut(s) 108, 749, 4127
Mph1103I ATGCAT 4 cut(s) 694, 5128, 5279, 5706
MroXI GAANNNNTTC 3 cut(s) 837, 3049, 5037
MscI TGGCCA 3 cut(s) 108, 749, 4127
MslI CAYNNNNRTG 8 cut(s) 489, 1409, 2348, 2898, 3065, 4825, 5082, 5282
Msp20I TGGCCA 3 cut(s) 108, 749, 4127
MspA1I CMGCKG 2 cut(s) 587, 5068
MspI CCGG 7 cut(s) 283, 299, 333, 527, 1745, 4547, 5177
MunI CAATTG 1 cut(s) 3087
Mva1269I GAATGC 5 cut(s) 1320, 2412, 3719, 6020, 6226
MvnI CGCG 3 cut(s) 19, 2687, 2755
NciI CCSGG 1 cut(s) 1745
NcoI CCATGG 1 cut(s) 3422
NdeI CATATG 3 cut(s) 616, 2677, 4513
NlaIV GGNNCC 9 cut(s) 42, 505, 2362, 2363, 3245, 3428, 3526, 3624, 5304
NmeAIII GCCGAG 1 cut(s) 5778
NsiI ATGCAT 4 cut(s) 694, 5128, 5279, 5706
NspI RCATGY 8 cut(s) 391, 696, 943, 1588, 1678, 3713, 5537, 5704
NspV TTCGAA 1 cut(s) 6216
OliI CACNNNNGTG 1 cut(s) 2348
PaeI GCATGC 3 cut(s) 943, 3713, 5704
PaeR7I CTCGAG 2 cut(s) 729, 4274
PagI TCATGA 3 cut(s) 484, 2488, 4993
PaqCI CACCTGC 1 cut(s) 785
PceI AGGCCT 3 cut(s) 62, 469, 4341
PciI ACATGT 2 cut(s) 1674, 5533
PciSI GCTCTTC 1 cut(s) 1164
PcsI WCGNNNNNNNCGW 1 cut(s) 140
PctI GAATGC 5 cut(s) 1320, 2412, 3719, 6020, 6226
PdmI GAANNNNTTC 3 cut(s) 837, 3049, 5037
PflFI GACNNNGTC 1 cut(s) 5597
PflMI CCANNNNNTGG 1 cut(s) 2384
PfoI TCCNGGA 1 cut(s) 4465
PinAI ACCGGT 1 cut(s) 4546
PleI GAGTC 7 cut(s) 139, 574, 1795, 1878, 2017, 2532, 4398
PpsI GAGTC 7 cut(s) 139, 574, 1795, 1878, 2017, 2532, 4398
Ppu21I YACGTR 2 cut(s) 3143, 5142
PpuMI RGGWCCY 2 cut(s) 1204, 2361
PscI ACATGT 2 cut(s) 1674, 5533
PshBI ATTAAT 1 cut(s) 5082
PsiI TTATAA 2 cut(s) 2477, 2651
Psp124BI GAGCTC 3 cut(s) 122, 2121, 3925
Psp5II RGGWCCY 2 cut(s) 1204, 2361
PspEI GGTNACC 1 cut(s) 1454
PspFI CCCAGC 5 cut(s) 2231, 2438, 3531, 3984, 5314
PspN4I GGNNCC 9 cut(s) 42, 505, 2362, 2363, 3245, 3428, 3526, 3624, 5304
PspPPI RGGWCCY 2 cut(s) 1204, 2361
PspXI VCTCGAGB 1 cut(s) 4274
PstI CTGCAG 3 cut(s) 1516, 4668, 5907
PstNI CAGNNNCTG 6 cut(s) 980, 1391, 1861, 2083, 2750, 5902
PsuI RGATCY 9 cut(s) 479, 1260, 2188, 2707, 2732, 3426, 4760, 5257, 5899
PsyI GACNNNGTC 1 cut(s) 5597
PvuII CAGCTG 1 cut(s) 5068
RseI CAYNNNNRTG 8 cut(s) 489, 1409, 2348, 2898, 3065, 4825, 5082, 5282
SacI GAGCTC 3 cut(s) 122, 2121, 3925
SalI GTCGAC 2 cut(s) 53, 4904
SapI GCTCTTC 1 cut(s) 1164
ScaI AGTACT 2 cut(s) 715, 2855
SchI GAGTC 7 cut(s) 140, 575, 1796, 1878, 2018, 2533, 4398
SexAI ACCWGGT 1 cut(s) 778
SfcI CTRYAG 5 cut(s) 1512, 4347, 4664, 5903, 5942
Sfr274I CTCGAG 2 cut(s) 729, 4274
SfuI TTCGAA 1 cut(s) 6216
SinI GGWCC 9 cut(s) 590, 808, 1204, 2361, 3026, 3229, 3380, 4597, 6067
SlaI CTCGAG 2 cut(s) 729, 4274
SmiI ATTTAAAT 1 cut(s) 2574
SmiMI CAYNNNNRTG 8 cut(s) 489, 1409, 2348, 2898, 3065, 4825, 5082, 5282
SmlI CTYRAG 7 cut(s) 631, 729, 1284, 2716, 2981, 3305, 4274
SmoI CTYRAG 7 cut(s) 631, 729, 1284, 2716, 2981, 3305, 4274
SpeI ACTAGT 3 cut(s) 895, 1369, 1759
SphI GCATGC 3 cut(s) 943, 3713, 5704
SseBI AGGCCT 3 cut(s) 62, 469, 4341
SspI AATATT 5 cut(s) 1111, 2950, 3215, 3407, 3654
SstI GAGCTC 3 cut(s) 122, 2121, 3925
StuI AGGCCT 3 cut(s) 62, 469, 4341
StyI CCWWGG 6 cut(s) 1132, 3422, 4484, 4631, 5844, 6070
SwaI ATTTAAAT 1 cut(s) 2574
TaiI ACGT 9 cut(s) 54, 2245, 2486, 2893, 3145, 4573, 5144, 6034, 6089
TatI WGTACW 8 cut(s) 713, 1347, 2303, 2813, 2853, 3069, 3840, 4811
TauI GCSGC 2 cut(s) 190, 1332
TspGWI ACGGA 2 cut(s) 3043, 5650
Tth111I GACNNNGTC 1 cut(s) 5597
Van91I CCANNNNNTGG 1 cut(s) 2384
VpaK11BI GGWCC 9 cut(s) 590, 808, 1204, 2361, 3026, 3229, 3380, 4597, 6067
VspI ATTAAT 1 cut(s) 5082
XagI CCTNNNNNAGG 2 cut(s) 1221, 4769
XbaI TCTAGA 2 cut(s) 3077, 3325
XceI RCATGY 8 cut(s) 391, 696, 943, 1588, 1678, 3713, 5537, 5704
XcmI CCANNNNNNNNNTGG 2 cut(s) 101, 3501
XhoI CTCGAG 2 cut(s) 729, 4274
XmiI GTMKAC 4 cut(s) 54, 4648, 4905, 5632
XmnI GAANNNNTTC 3 cut(s) 837, 3049, 5037
ZraI GACGTC 1 cut(s) 52
ZrmI AGTACT 2 cut(s) 715, 2855
Zsp2I ATGCAT 4 cut(s) 694, 5128, 5279, 5706
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.