Rw7G004460

Activating signal cointegrator 1 complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0008552
Physical Location & Seq
Reverse (-)
3731901 .. 3753148
21248 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G004460.1

Sequence Viewer

Length: 5751 bp
ATGCTGGTTCAGCTTCCGCGATTAACTAGCTCTCTGAGGGAGCCCTTCGACGTCGACCAGGCCTATTTGCAGCGCAAGCTCATCCTTCAAAACCACACCAAGCCTCGCCAATCATCGAGCTCAGTTGATGAGTCGGAGCTTGCGAGAAAGATAGTTCATAGATGGGAGGAAGCTTCGTATGAGCTGCGGCAAGCTTACAAGCAGTTTATTGGAGCTGTGGTGGAGTTGATAGATGGAGAAGTTCAATCCGAGGAGTTTCGTGAGGTGGCACTCGTTGTTTACCGGCTTTTTGGCAGACCGGAGGAGGAATGCAGTGTTGAAACAAATTTTGCCGGAAAAAAGCAGGAAGTGCAAATGCTTTTGGGGCACACAGTTTCAGATGTTAACATGCGGAAAGTTGCGTCTCTAGCTCAGAGACTTTGTGGGATGCAGTCTAGTGATCATGGGATTACACTTGTTTCAGAAAGGCCTGTAAGTGGATCTTATGACGATGCAGAATTTGGTGCCGACCTTGTTTTTCATCCTCCGGCTCGGTTCTTGGTAGATGTTTCTTTGGATGATGCGGAGTCATTTTGTGAGGAAACCGCTGGACCTTCCTCTTATTATGAAGGATCATATGGTCACAGTGGCTTGAGTGGCGGTCATTCTGCTACTGATGGGCGCAGCATCGATCTGACTTGGCTACAAGATGCATGTGATCAGATAACAAAAAGTACTACACAGCTTTCTCGAGATGAACTGGCTATGGCCATTTGTCGGGTGCTTGATTCTGACAAACCTGGTGACGAGATAGCAGGTGACTTGTTGGACCTTGTGGGGGATAGTGCATTTGAAACTGTTCAAGACCTCATATCACATCGAAAGGAACTTGTTGATGCGATCCATCATGGGTTACTAGTAATGAAATCTGACAAATCGTCTTTGACCTCTCAACCTCGCATGCCGAGTTATGGCACACAGGTTACTGTTCAAACAGAATCTGAACGGCAGATTGATAAGCTTCGGCGCAAAGAGGAAAAGCGAAATAGACGAGGAACAGAATATGGCACTGATAGTGATCTGGCTGCTGTAAATTTTTCTTCTCTACTACAAGCAAGTGAGAAAAAAAATATTTTTGATGATCTATCGGGCCTTGGTGAAGGGATAGCAGTTAATGCTCTTCCGCAAGGAACCCAGAGGAAGCACCACAAGGGGTATGAGGAGGTCCTAATTCCTCCAACACCAGGAGCACAAATGAAACCTGGAGAAAAGCTGATTGAGATCTCGGAGTTAGATGAGTTTGCTCAAGCTGCTTTTCGTGGATACAAATCTTTGAACCGCATTCAGAGCCGCATATATCATACTGTGTACTACACAAATGAGAATATACTAGTTTGTGCTCCAACAGGAGCTGGCAAAACAAACATAGCTATGATTTCCATTCTACATGAGATTGGACAGCACTTCAAAGATGGTTACCTGCACAAAGACGAGTTCAAAATAGTTTATGTTGCACCTATGAAGGCGTTAGCTGCAGAAGTCACATCAACTTTTAGCCATCGGTTATCTCCATTGAATATGACTGTGAGAGAACTTACTGGAGACATGCAACTTTCTAAGAATGAACTTGAAGAAACTCAGATGATAGTAACAACACCTGAGAAATGGGATGTCATTACTCGCAAGAGCAGTGACATGTCACTTTCAATGCTGGTGAAGCTCCTAATAATTGATGAAGTTCATCTTTTGAATGACGATAGAGGCCCGGTAATTGAGGCACTAGTTGCTCGGACTTTACGCCAGGTGGAGTCTTCACAGTCAATGATACGCATTGTGGGACTTTCCGCCACTCTACCCAACTATTTGGAGGTTGCACAGTTTCTGAGAGTGAATCCAGAGGCAGGACTCTTCTACTTTGATTCTAGCTATCGTCCAGTGCCCCTTGCACAACAGTATATTGGTATTAGTGAGACAAACTTTTCAGCCAAAACTGAATTGTTGAATGAGATATGCTACAAGAAGGTTGTGGAGTCACTAAGGCAAGGTCATCAGGCAATGGTATTTGTCCATTCTCGAAAGGACACAGCAAAAACGGCTCAGAAGCTGGTTGAACTTGCTCGCAAATTTGAAGGACTGGAGCTCTTTAAGAATGATCAGCACCCACAATTTTCATTGATCCAGAGGGAAGTTGTAAAATCCAGAAACAAAGATCTTGTTGAACTTTTTGAATTTGGAATTGGAATTCATAATGCTGGGATGTTACGTTCTGATAGAGGTCTGACCGAGCGCCTTTTCTCTGATGGACTATTGAAGGTACTTGTTTGTACAGCAACTTTGGCGTGGGGTGTAAATCTACCAGCTCACACTGTTGTGATAAAGGGGACCCAATTATATGATCCAAAGGCTGGTGGGTGGCGAGACCTGGGAATGCTTGATGTTATGCAGATATTTGGACGGGCTGGGAGACCTCAATTCGATAAAAGTGGTGAAGGTATTATAATCACTTCTCATGACAAGCTGGCCTACTATCTGCGGTTGCTGACGAGTCAACTTCCTATTGAAAGTCAGTTCATTAGTTCCTTGAAGGACAATTTAAATGCAGAGGTGGCACTTGGAACTGTCACCAATGTCAAGGAAGCTTGTGCATGGCTTGGATATACATACCTTTTTATAAGGATGAGGCTCAACCCTTTGGCATATGGTATCGCGTGGGAGGAGGTTATGGCAGATCCTTCCTTGAATTTAAAGCAGAGATCTCTCGTAGCAGATGCTGCACGTGCCCTTGACAAAGCAAAAATGATGCGGTTTGACGAGAAAAGTGGCAATTTTTATTGTACAGAGCTTGGTCGCATAGCAAGCCACTTTTATATTCAATACTCTAGTGTTGAAACCTACAATGAAATGTTGAAACGTCACATGAATGAGACTGAGGTAATTGACATGGTAGCCCACTCCTCAGAATTTGAAAATATTGTTATTCGAGAAGAGGAACAAAATGAGCTTGAGATGTTGGTGCGAAAATTGTGTCCAGTAGAAGTTAGGGGTGGTCCTTCAAATAAACATGGAAAGATTTCCGTTCTCATTCAGGTGTACATATCTAGAGGCTCAATTGATACCTTTTCTTTGGTTTCTGATGCTCAATATATAAGTGCAAGCTTGGCACGTATTATGCGGGCCTTATTTGAGATTTGTTTGCGAAAAGGCTGGAGTGAGATGTCATTGTTCATGTTGGAATATTGCAAGGCAGTGGACCGCCAAGTTTGGCCCCATCAACATCCTTTCAGACAATTTGACAGGGAGATTTCAGCACAAATACTGCGAAATCTTGAGGAACGAGGTGCTGATCTAGATCGCCTATATGATATGGAGGAAAAAGAGATTGGGAAACTGGTCAATTATGGACCTGGAGGAAGGTTGGTTAAGCAATATTTGGGATATTTTCCATGGATCCAGTTAGCAGCAACTGTAAGTCCAATTACCAGAACTGTTTTAAAGGTGGATTTGCATATTAAACCAGACTTTATATGGAAAGATCGTTTTCACGGCACCGCCCAGCGTTGGTGGATTCTAGTTGAGGATTCTGAGAATGATCATATCTACCACTCAGAGCTATTCACTCTAACAAAGCGGATGGCTAAAGGGGAACCTCAGAAGCTCTCGTTCACTGTGCCAATATTTGAGCCACATCCGCCACAGTATTATATACGTGCTGTTTCTGATTCTTGGTTGCATGCAGAGGCATTCTACACCATTTCTTTCCATAATCTAACACTGCCAGAGGCCCATACTTCACATACAGAACTGCTAGATTTAAAGCCTCTCCCTGTGACATCTCTTGGAAACACTGCTTATGAAGCATTGTACAAATTCTCGCACTTTAATCCAATACAAACACAGACTTTCCATGTGCTGTATCACACAGACAATAATGTATTACTGGGAGCTCCTACTGGAAGTGGAAAAACTATTTCTGCCGAGCTTGCTATGCTTCGTCTCTTCAACACCCAGCCTGATATGAAGGTCATTTACATAGCACCTTTGAAGGCTATTGTTCGAGAAAGAATGAACGATTGGAGGAAGCGCCTTGTCTCTCAGCTAGGGAAGAAAATGGTTGAAATGACAGGAGATTATACTCCAGATTTGATGGCCATCTTGTCAGCAGATATCATAATATCAACTCCTGAAAAATGGGATGGTATCAGTCGTAATTGGCATAGCCGAACCTATGTTAAAAAGGTTGGGCTTATGATTTTGGATGAGATTCACTTGTTGGGAGCTGACCGTGGGCCTATCCTCGAGGTTATTGTGTCAAGGATGAGATACATATCCTCACAGACAGAGCGTGAAGTACGGTTTGTAGGCCTTTCTACAGCTCTGGCAAATGCAGGAGATTTAGCTGATTGGTTGGGTGTTGGAGAAACTGGACTCTTCAATTTCAAACCAAGTGTGAGACCTGTACCTCTTGAAGTTCATATCCAGGGATATCCAGGAAAGTTCTATTGTCCAAGGATGAATAGCATGAACAAACCATCATATGCTGCTATATGCACCCATTCACCTACAAAACCGGTTCTAATTTTTGTTTCCTCACGTCGCCAGACAAGGCTTACTGCATTGGACCTCATTCAGTATGCAGCTTCAGATGAGCATCCAAGGCAGTTTCTTTGTATACCAGAAGAAGAACTGCAGATGGTTCTTTATCAGGTTGCTGATAGTAACCTGAGGCACACCTTGCAATTTGGCATTGGATTACACCATGCTGGTCTGAATGACAAAGATAGATCCCTGGTTGAGGAACTTTTTGCAAACAACAAGATTCAGGTATTGGTTTGTACTAGCACGTTGGCATGGGGTGTAAACCTTCCAGCGCATCTGGTTATTATTAAGGGAACAGAATTTTATGATGGGAAAACAAAAAGATATGTCGACTTCCCAATCACAGATATCTTGCAAATGATGGGTCGTGCAGGTCGCCCACAATTTGATCAACACGGGAAAGCAGTCATTCTTGTTCATGAACCCAAAAAGAGCTTCTATAAAAAGTTTTTATATGAACCATTTCCTGTCGAGAGTAGTCTGAGGGAACAGCTGCATAATCACATTAATGCGGAGATAGTTTCTGGCACAATTTGCCATAAAGAGGATGCATTGCATTATCTTACGTGGACATATTTATTCCGCAGACTGATGTTTAATCCGGCTTACTATGGCTTGGACAATACTGACGCCGAAGTTCTCAGTTCTTATTTGTCTAGGTTAGTGCAGAACACATTGGAGGATCTGGAAGACAGTGGATGCATCAAGATGAGTGAAGACAGTGTGGAGCCAATGATGCTGGGTTCGATAGCATCCCAGTATTACCTTAGCTACTTGACTGTATCGATGTTTGGTTCAAACATTGGCTCAGATACCTCTCTTGAAGTATTCTTGCATATCCTATCTGCTGCTTCTGAATATGATGAACTTCCAGTTCGCCATAACGAGGAAAATTACAATGAGGCATTGTCTGAGAGAGTTCGATATAAAGTGGATAAGGATCGCCTAGATGATCCACATGTCAAAGCAAATCTGCTATTTCAGGCACATTTCTCGCAGTTAGAATTACCAATTACTGACTATGTCACAGACTTGAAGTCAGTTTTGGATCAAAGTATACGGATTATCCAGGCCATGATAGATATATGTGCCAACAGTGGGTGGCTTTCAAGTTCAGTTACTTGCATGCATCTTTTGCAGATGGTCATGCAGGTGAAAGGCCTACAAACCTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DEAD Helicase_C Sec63

Protein Analysis

1916

Amino Acids

217.19

Weight (kDa)

5.94

Isoelectric Point (pI)

40.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DExH14_plug PF24557 45 - 144 3.1e-43 DExH14 plug domain
DEAD PF00270 439 - 613 1.4e-30 DEAD/DEAH box helicase
ResIII PF04851 446 - 612 2.8e-12 Type III restriction enzyme, res subunit
Helicase_C PF00271 730 - 816 3.3e-06 Helicase conserved C-terminal domain
WHD_SNRNP200 PF23445 847 - 918 5.7e-15 Winged Helix-turn-helix domain
Sec63 PF02889 938 - 1245 4.4e-88 Sec63 Brl domain
DEAD PF00270 1287 - 1453 2.3e-28 DEAD/DEAH box helicase
ResIII PF04851 1295 - 1420 6e-11 Type III restriction enzyme, res subunit
Helicase_C PF00271 1565 - 1652 2.1e-09 Helicase conserved C-terminal domain
WHD_SNRNP200 PF23445 1683 - 1763 7.3e-32 Winged Helix-turn-helix domain
Sec63 PF02889 1769 - 1909 1.8e-46 Sec63 Brl domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 2477, 2651
AarI CACCTGC 2 cut(s) 785, 5716
AatII GACGTC 1 cut(s) 54
AbsI CCTCGAGG 1 cut(s) 4274
Acc36I ACCTGC 4 cut(s) 785, 1467, 4937, 5716
AccB1I GGYRCC 2 cut(s) 503, 3524
AccB7I CCANNNNNTGG 1 cut(s) 2384
AccI GTMKAC 4 cut(s) 54, 4648, 4905, 5632
AccII CGCG 2 cut(s) 19, 2687
AcoI YGGCCR 2 cut(s) 747, 4125
AcuI CTGAAG 1 cut(s) 4602
AcvI CACGTG 1 cut(s) 2756
AcyI GRCGYC 2 cut(s) 51, 5205
AflIII ACRYGT 2 cut(s) 1674, 5533
AgeI ACCGGT 1 cut(s) 4546
AhdI GACNNNNNGTC 2 cut(s) 916, 5611
AhlI ACTAGT 3 cut(s) 895, 1369, 1759
AjiI CACGTC 1 cut(s) 4571
AleI CACNNNNGTG 1 cut(s) 2348
AloI GAACNNNNNNTCC 2 cut(s) 2227, 2259
Alw21I GWGCWC 5 cut(s) 122, 1231, 1381, 2121, 3925
AlwNI CAGNNNCTG 6 cut(s) 980, 1391, 1861, 2083, 2750, 3443
Ama87I CYCGRG 2 cut(s) 729, 4274
ArsI GACNNNNNNTTYG 4 cut(s) 2989, 3021, 4924, 4956
AseI ATTAAT 1 cut(s) 5082
AsiGI ACCGGT 1 cut(s) 4546
Asp700I GAANNNNTTC 3 cut(s) 837, 3049, 5037
AspLEI GCGC 6 cut(s) 75, 663, 1008, 2268, 4062, 4849
AsuC2I CCSGG 1 cut(s) 1745
AsuHPI GGTGA 8 cut(s) 794, 809, 1148, 1705, 2477, 2593, 4527, 5740
AvaI CYCGRG 2 cut(s) 729, 4274
AvaII GGWCC 8 cut(s) 590, 808, 1204, 2361, 3026, 3229, 3380, 4597
AxyI CCTNAGG 1 cut(s) 4700
BaeGI GKGCMC 3 cut(s) 369, 1920, 2761
BalI TGGCCA 2 cut(s) 749, 4127
BamHI GGATCC 1 cut(s) 3426
BanI GGYRCC 2 cut(s) 503, 3524
BanII GRGCYC 4 cut(s) 45, 122, 2121, 3925
BarI GAAGNNNNNNTAC 2 cut(s) 3989, 4021
BbrPI CACGTG 1 cut(s) 2756
BbsI GAAGAC 3 cut(s) 1782, 5271, 5298
Bbv12I GWGCWC 5 cut(s) 122, 1231, 1381, 2121, 3925
BceAI ACGGC 3 cut(s) 1001, 2088, 3538
BcgI CGANNNNNNTGC 2 cut(s) 5550, 5584
BciVI GTATCC 1 cut(s) 1295
BclI TGATCA 5 cut(s) 439, 697, 2131, 3568, 4963
BcnI CCSGG 1 cut(s) 1745
BcuI ACTAGT 3 cut(s) 895, 1369, 1759
BfmI CTRYAG 3 cut(s) 1512, 4347, 4664
BfoI RGCGCY 2 cut(s) 2269, 4063
BfuAI ACCTGC 4 cut(s) 785, 1467, 4937, 5716
BfuI GTATCC 1 cut(s) 1295
BglII AGATCT 3 cut(s) 1260, 2188, 2732
BmcAI AGTACT 1 cut(s) 715
Bme18I GGWCC 8 cut(s) 590, 808, 1204, 2361, 3026, 3229, 3380, 4597
BmeRI GACNNNNNGTC 2 cut(s) 916, 5611
BmeT110I CYCGRG 2 cut(s) 729, 4274
BmgBI CACGTC 1 cut(s) 4571
BmrI ACTGGG 2 cut(s) 3926, 5326
BmuI ACTGGG 2 cut(s) 3926, 5326
BpiI GAAGAC 3 cut(s) 1782, 5271, 5298
BpmI CTGGAG 6 cut(s) 1263, 1599, 2135, 3205, 3405, 4098
Bpu10I CCTNAGC 1 cut(s) 5342
BpuEI CTTGAG 4 cut(s) 652, 1269, 3002, 3326
BpuMI CCSGG 1 cut(s) 1745
Bsa29I ATCGAT 2 cut(s) 669, 5360
BsaAI YACGTR 4 cut(s) 2756, 3143, 3686, 5142
BsaBI GATNNNNATC 8 cut(s) 1056, 1259, 1306, 3327, 4127, 4303, 4626, 5514
BsaHI GRCGYC 2 cut(s) 51, 5205
BsaI GGTCTC 3 cut(s) 2391, 2437, 4423
BsaJI CCNNGG 9 cut(s) 249, 1132, 2401, 3422, 4261, 4456, 4484, 4631, 4764
BsaWI WCCGGW 2 cut(s) 298, 4546
BsaXI ACNNNNNCTCC 2 cut(s) 204, 234
Bse118I RCCGGY 2 cut(s) 282, 4546
Bse21I CCTNAGG 1 cut(s) 4700
Bse3DI GCAATG 2 cut(s) 2040, 5126
Bse8I GATNNNNATC 8 cut(s) 1056, 1259, 1306, 3327, 4127, 4303, 4626, 5514
BseCI ATCGAT 2 cut(s) 669, 5360
BseDI CCNNGG 9 cut(s) 249, 1132, 2401, 3422, 4261, 4456, 4484, 4631, 4764
BseJI GATNNNNATC 8 cut(s) 1056, 1259, 1306, 3327, 4127, 4303, 4626, 5514
BseMI GCAATG 2 cut(s) 2040, 5126
BseRI GAGGAG 5 cut(s) 266, 317, 1214, 2708, 2923
BseSI GKGCMC 3 cut(s) 369, 1920, 2761
BseYI CCCAGC 5 cut(s) 2231, 2438, 3531, 3984, 5314
BsgI GTGCAG 4 cut(s) 1445, 2736, 4965, 5261
Bsh1236I CGCG 2 cut(s) 19, 2687
BshNI GGYRCC 2 cut(s) 503, 3524
BshTI ACCGGT 1 cut(s) 4546
BshVI ATCGAT 2 cut(s) 669, 5360
BsiHKAI GWGCWC 5 cut(s) 122, 1231, 1381, 2121, 3925
BsiHKCI CYCGRG 2 cut(s) 729, 4274
BsiSI CCGG 7 cut(s) 283, 299, 333, 527, 1745, 4547, 5177
BslFI GGGAC 2 cut(s) 1830, 2374
BsmBI CGTCTC 2 cut(s) 408, 3977
BsmFI GGGAC 2 cut(s) 1830, 2374
BsmI GAATGC 4 cut(s) 314, 1320, 2412, 3719
Bso31I GGTCTC 3 cut(s) 2391, 2437, 4423
BsoBI CYCGRG 2 cut(s) 729, 4274
Bsp1286I GDGCHC 9 cut(s) 45, 122, 369, 1231, 1381, 1920, 2121, 2761, 3925
Bsp1407I TGTACA 4 cut(s) 2303, 2813, 3069, 3840
Bsp19I CCATGG 1 cut(s) 3422
BspDI ATCGAT 2 cut(s) 669, 5360
BspFNI CGCG 2 cut(s) 19, 2687
BspHI TCATGA 2 cut(s) 2488, 4993
BspMAI CTGCAG 2 cut(s) 1516, 4668
BspMI ACCTGC 4 cut(s) 785, 1467, 4937, 5716
BspQI GCTCTTC 1 cut(s) 1164
BspT107I GGYRCC 2 cut(s) 503, 3524
BspTNI GGTCTC 3 cut(s) 2391, 2437, 4423
BsrDI GCAATG 2 cut(s) 2040, 5126
BsrFI RCCGGY 2 cut(s) 282, 4546
BsrGI TGTACA 4 cut(s) 2303, 2813, 3069, 3840
BssAI RCCGGY 2 cut(s) 282, 4546
BssECI CCNNGG 9 cut(s) 249, 1132, 2401, 3422, 4261, 4456, 4484, 4631, 4764
BssNAI GTATAC 2 cut(s) 4649, 5633
BssNI GRCGYC 2 cut(s) 51, 5205
BssT1I CCWWGG 4 cut(s) 1132, 3422, 4484, 4631
Bst1107I GTATAC 2 cut(s) 4649, 5633
Bst6I CTCTTC 5 cut(s) 1164, 1892, 2958, 3980, 4412
BstACI GRCGYC 2 cut(s) 51, 5205
BstAPI GCANNNNNTGC 7 cut(s) 349, 1154, 1763, 2750, 4711, 5109, 5710
BstAUI TGTACA 4 cut(s) 2303, 2813, 3069, 3840
BstBAI YACGTR 4 cut(s) 2756, 3143, 3686, 5142
BstDSI CCRYGG 2 cut(s) 3422, 4261
BstEII GGTNACC 1 cut(s) 1454
BstENI CCTNNNNNAGG 1 cut(s) 4769
BstFNI CGCG 2 cut(s) 19, 2687
BstH2I RGCGCY 2 cut(s) 2269, 4063
BstHHI GCGC 6 cut(s) 75, 663, 1008, 2268, 4062, 4849
BstNSI RCATGY 8 cut(s) 391, 696, 943, 1588, 1678, 3713, 5537, 5704
BstPI GGTNACC 1 cut(s) 1454
BstSFI CTRYAG 3 cut(s) 1512, 4347, 4664
BstSLI GKGCMC 3 cut(s) 369, 1920, 2761
BstUI CGCG 2 cut(s) 19, 2687
BstV2I GAAGAC 3 cut(s) 1782, 5271, 5298
BstX2I RGATCY 8 cut(s) 479, 1260, 2188, 2707, 2732, 3426, 4760, 5257
BstXI CCANNNNNNTGG 1 cut(s) 1843
BstYI RGATCY 8 cut(s) 479, 1260, 2188, 2707, 2732, 3426, 4760, 5257
BstZ17I GTATAC 2 cut(s) 4649, 5633
Bsu15I ATCGAT 2 cut(s) 669, 5360
Bsu36I CCTNAGG 1 cut(s) 4700
BsuI GTATCC 1 cut(s) 1295
BsuTUI ATCGAT 2 cut(s) 669, 5360
BtgI CCRYGG 2 cut(s) 3422, 4261
BtrI CACGTC 1 cut(s) 4571
BtsI GCAGTG 5 cut(s) 319, 1675, 3231, 3749, 3822
BveI ACCTGC 4 cut(s) 785, 1467, 4937, 5716
CaiI CAGNNNCTG 6 cut(s) 980, 1391, 1861, 2083, 2750, 3443
CciI TCATGA 2 cut(s) 2488, 4993
CfoI GCGC 6 cut(s) 75, 663, 1008, 2268, 4062, 4849
Cfr10I RCCGGY 2 cut(s) 282, 4546
ClaI ATCGAT 2 cut(s) 669, 5360
CseI GACGC 2 cut(s) 390, 5213
CsiI ACCWGGT 1 cut(s) 778
CspAI ACCGGT 1 cut(s) 4546
DraI TTTAAA 4 cut(s) 2574, 2724, 3471, 3792
DriI GACNNNNNGTC 2 cut(s) 916, 5611
EaeI YGGCCR 2 cut(s) 747, 4125
Eam1104I CTCTTC 5 cut(s) 1164, 1892, 2958, 3980, 4412
Eam1105I GACNNNNNGTC 2 cut(s) 916, 5611
EarI CTCTTC 5 cut(s) 1164, 1892, 2958, 3980, 4412
EciI GGCGGA 2 cut(s) 1813, 3657
Ecl136II GAGCTC 3 cut(s) 120, 2119, 3923
Eco130I CCWWGG 4 cut(s) 1132, 3422, 4484, 4631
Eco147I AGGCCT 4 cut(s) 62, 469, 4341, 5736
Eco24I GRGCYC 4 cut(s) 45, 122, 2121, 3925
Eco31I GGTCTC 3 cut(s) 2391, 2437, 4423
Eco32I GATATC 3 cut(s) 4144, 4463, 4924
Eco47I GGWCC 8 cut(s) 590, 808, 1204, 2361, 3026, 3229, 3380, 4597
Eco53kI GAGCTC 3 cut(s) 120, 2119, 3923
Eco57I CTGAAG 1 cut(s) 4602
Eco72I CACGTG 1 cut(s) 2756
Eco81I CCTNAGG 1 cut(s) 4700
Eco88I CYCGRG 2 cut(s) 729, 4274
Eco91I GGTNACC 1 cut(s) 1454
EcoICRI GAGCTC 3 cut(s) 120, 2119, 3923
EcoNI CCTNNNNNAGG 1 cut(s) 4769
EcoO109I RGGNCCY 2 cut(s) 1204, 2361
EcoO65I GGTNACC 1 cut(s) 1454
EcoRI GAATTC 1 cut(s) 2220
EcoRV GATATC 3 cut(s) 4144, 4463, 4924
EcoT14I CCWWGG 4 cut(s) 1132, 3422, 4484, 4631
EcoT22I ATGCAT 4 cut(s) 694, 5128, 5279, 5706
EcoT38I GRGCYC 4 cut(s) 45, 122, 2121, 3925
ErhI CCWWGG 4 cut(s) 1132, 3422, 4484, 4631
Esp3I CGTCTC 2 cut(s) 408, 3977
FalI AAGNNNNNCTT 4 cut(s) 466, 498, 1707, 1739
FaqI GGGAC 2 cut(s) 1830, 2374
FauI CCCGC 1 cut(s) 3144
FauNDI CATATG 3 cut(s) 616, 2677, 4513
FbaI TGATCA 5 cut(s) 439, 697, 2131, 3568, 4963
FblI GTMKAC 4 cut(s) 54, 4648, 4905, 5632
FriOI GRGCYC 4 cut(s) 45, 122, 2121, 3925
GlaI GCGC 6 cut(s) 74, 662, 1007, 2267, 4061, 4848
GsaI CCCAGC 5 cut(s) 2235, 2442, 3535, 3988, 5318
GsuI CTGGAG 6 cut(s) 1263, 1599, 2135, 3205, 3405, 4098
HaeII RGCGCY 2 cut(s) 2269, 4063
HapII CCGG 7 cut(s) 283, 299, 333, 527, 1745, 4547, 5177
HgaI GACGC 2 cut(s) 390, 5213
HhaI GCGC 6 cut(s) 75, 663, 1008, 2268, 4062, 4849
Hin1I GRCGYC 2 cut(s) 51, 5205
Hin6I GCGC 6 cut(s) 73, 661, 1006, 2266, 4060, 4847
HinP1I GCGC 6 cut(s) 73, 661, 1006, 2266, 4060, 4847
HincII GTYRAC 4 cut(s) 55, 385, 2528, 4906
HindII GTYRAC 4 cut(s) 55, 385, 2528, 4906
HindIII AAGCTT 5 cut(s) 171, 192, 998, 2616, 3133
HpaI GTTAAC 1 cut(s) 385
HpaII CCGG 7 cut(s) 283, 299, 333, 527, 1745, 4547, 5177
HphI GGTGA 8 cut(s) 794, 809, 1148, 1705, 2477, 2593, 4527, 5740
Hpy99I CGWCG 3 cut(s) 53, 56, 4575
HpyCH4IV ACGT 9 cut(s) 51, 2242, 2755, 2890, 3142, 3685, 4570, 4820, 5141
HpySE526I ACGT 9 cut(s) 51, 2242, 2755, 2890, 3142, 3685, 4570, 4820, 5141
Hsp92I GRCGYC 2 cut(s) 51, 5205
HspAI GCGC 6 cut(s) 73, 661, 1006, 2266, 4060, 4847
KflI GGGWCCC 1 cut(s) 2361
Ksp22I TGATCA 5 cut(s) 439, 697, 2131, 3568, 4963
KspAI GTTAAC 1 cut(s) 385
LguI GCTCTTC 1 cut(s) 1164
MabI ACCWGGT 1 cut(s) 778
MaeII ACGT 9 cut(s) 51, 2242, 2755, 2890, 3142, 3685, 4570, 4820, 5141
MfeI CAATTG 1 cut(s) 3087
MflI RGATCY 8 cut(s) 479, 1260, 2188, 2707, 2732, 3426, 4760, 5257
MhlI GDGCHC 9 cut(s) 45, 122, 369, 1231, 1381, 1920, 2121, 2761, 3925
MlsI TGGCCA 2 cut(s) 749, 4127
MluNI TGGCCA 2 cut(s) 749, 4127
MlyI GAGTC 7 cut(s) 140, 575, 1796, 1878, 2018, 2533, 4398
MmeI TCCRAC 6 cut(s) 114, 786, 1241, 1406, 3189, 4372
Mox20I TGGCCA 2 cut(s) 749, 4127
Mph1103I ATGCAT 4 cut(s) 694, 5128, 5279, 5706
MroXI GAANNNNTTC 3 cut(s) 837, 3049, 5037
MscI TGGCCA 2 cut(s) 749, 4127
MslI CAYNNNNRTG 7 cut(s) 1409, 2348, 2898, 3065, 5082, 5282, 5726
Msp20I TGGCCA 2 cut(s) 749, 4127
MspA1I CMGCKG 2 cut(s) 587, 5068
MspI CCGG 7 cut(s) 283, 299, 333, 527, 1745, 4547, 5177
MunI CAATTG 1 cut(s) 3087
Mva1269I GAATGC 4 cut(s) 314, 1320, 2412, 3719
MvnI CGCG 2 cut(s) 19, 2687
NciI CCSGG 1 cut(s) 1745
NcoI CCATGG 1 cut(s) 3422
NdeI CATATG 3 cut(s) 616, 2677, 4513
NmeAIII GCCGAG 2 cut(s) 969, 3979
NsiI ATGCAT 4 cut(s) 694, 5128, 5279, 5706
NspI RCATGY 8 cut(s) 391, 696, 943, 1588, 1678, 3713, 5537, 5704
OliI CACNNNNGTG 1 cut(s) 2348
PaeI GCATGC 3 cut(s) 943, 3713, 5704
PaeR7I CTCGAG 2 cut(s) 729, 4274
PagI TCATGA 2 cut(s) 2488, 4993
PaqCI CACCTGC 2 cut(s) 785, 5716
PceI AGGCCT 4 cut(s) 62, 469, 4341, 5736
PciI ACATGT 2 cut(s) 1674, 5533
PciSI GCTCTTC 1 cut(s) 1164
PcsI WCGNNNNNNNCGW 1 cut(s) 140
PctI GAATGC 4 cut(s) 314, 1320, 2412, 3719
PdmI GAANNNNTTC 3 cut(s) 837, 3049, 5037
PfeI GAWTC 9 cut(s) 767, 977, 1870, 1898, 3544, 3557, 3698, 4240, 4795
PflFI GACNNNGTC 1 cut(s) 5597
PflMI CCANNNNNTGG 1 cut(s) 2384
PfoI TCCNGGA 1 cut(s) 4465
PinAI ACCGGT 1 cut(s) 4546
PleI GAGTC 7 cut(s) 139, 574, 1795, 1878, 2017, 2532, 4398
PmaCI CACGTG 1 cut(s) 2756
PmlI CACGTG 1 cut(s) 2756
PpsI GAGTC 7 cut(s) 139, 574, 1795, 1878, 2017, 2532, 4398
Ppu21I YACGTR 4 cut(s) 2756, 3143, 3686, 5142
PpuMI RGGWCCY 2 cut(s) 1204, 2361
PscI ACATGT 2 cut(s) 1674, 5533
PshBI ATTAAT 1 cut(s) 5082
PsiI TTATAA 2 cut(s) 2477, 2651
Psp124BI GAGCTC 3 cut(s) 122, 2121, 3925
Psp5II RGGWCCY 2 cut(s) 1204, 2361
PspCI CACGTG 1 cut(s) 2756
PspEI GGTNACC 1 cut(s) 1454
PspFI CCCAGC 5 cut(s) 2231, 2438, 3531, 3984, 5314
PspPPI RGGWCCY 2 cut(s) 1204, 2361
PspXI VCTCGAGB 1 cut(s) 4274
PstI CTGCAG 2 cut(s) 1516, 4668
PstNI CAGNNNCTG 6 cut(s) 980, 1391, 1861, 2083, 2750, 3443
PsuI RGATCY 8 cut(s) 479, 1260, 2188, 2707, 2732, 3426, 4760, 5257
PsyI GACNNNGTC 1 cut(s) 5597
PvuII CAGCTG 1 cut(s) 5068
RseI CAYNNNNRTG 7 cut(s) 1409, 2348, 2898, 3065, 5082, 5282, 5726
SacI GAGCTC 3 cut(s) 122, 2121, 3925
SalI GTCGAC 2 cut(s) 53, 4904
SapI GCTCTTC 1 cut(s) 1164
ScaI AGTACT 1 cut(s) 715
SchI GAGTC 7 cut(s) 140, 575, 1796, 1878, 2018, 2533, 4398
SduI GDGCHC 9 cut(s) 45, 122, 369, 1231, 1381, 1920, 2121, 2761, 3925
SexAI ACCWGGT 1 cut(s) 778
SfcI CTRYAG 3 cut(s) 1512, 4347, 4664
Sfr274I CTCGAG 2 cut(s) 729, 4274
SinI GGWCC 8 cut(s) 590, 808, 1204, 2361, 3026, 3229, 3380, 4597
SlaI CTCGAG 2 cut(s) 729, 4274
SmiI ATTTAAAT 1 cut(s) 2574
SmiMI CAYNNNNRTG 7 cut(s) 1409, 2348, 2898, 3065, 5082, 5282, 5726
SmlI CTYRAG 6 cut(s) 631, 729, 1284, 2981, 3305, 4274
SmoI CTYRAG 6 cut(s) 631, 729, 1284, 2981, 3305, 4274
SpeI ACTAGT 3 cut(s) 895, 1369, 1759
SphI GCATGC 3 cut(s) 943, 3713, 5704
SseBI AGGCCT 4 cut(s) 62, 469, 4341, 5736
SspI AATATT 5 cut(s) 1111, 2950, 3215, 3407, 3654
SstI GAGCTC 3 cut(s) 122, 2121, 3925
StuI AGGCCT 4 cut(s) 62, 469, 4341, 5736
StyI CCWWGG 4 cut(s) 1132, 3422, 4484, 4631
SwaI ATTTAAAT 1 cut(s) 2574
TaiI ACGT 9 cut(s) 54, 2245, 2758, 2893, 3145, 3688, 4573, 4823, 5144
TaqII GACCGA 1 cut(s) 2276
TatI WGTACW 7 cut(s) 713, 1347, 2303, 2813, 3069, 3840, 4811
TauI GCSGC 2 cut(s) 190, 1332
TfiI GAWTC 9 cut(s) 767, 977, 1870, 1898, 3544, 3557, 3698, 4240, 4795
TspGWI ACGGA 2 cut(s) 3043, 5650
Tth111I GACNNNGTC 1 cut(s) 5597
Van91I CCANNNNNTGG 1 cut(s) 2384
VpaK11BI GGWCC 8 cut(s) 590, 808, 1204, 2361, 3026, 3229, 3380, 4597
VspI ATTAAT 1 cut(s) 5082
XagI CCTNNNNNAGG 1 cut(s) 4769
XbaI TCTAGA 2 cut(s) 3077, 3325
XceI RCATGY 8 cut(s) 391, 696, 943, 1588, 1678, 3713, 5537, 5704
XcmI CCANNNNNNNNNTGG 1 cut(s) 3501
XhoI CTCGAG 2 cut(s) 729, 4274
XmiI GTMKAC 4 cut(s) 54, 4648, 4905, 5632
XmnI GAANNNNTTC 3 cut(s) 837, 3049, 5037
ZraI GACGTC 1 cut(s) 52
ZrmI AGTACT 1 cut(s) 715
Zsp2I ATGCAT 4 cut(s) 694, 5128, 5279, 5706
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.