RchiOBHm_Chr7g0182981

Activating signal cointegrator 1 complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0008552
Physical Location & Seq
Reverse (-)
4021413 .. 4039448
18036 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ16322

Sequence Viewer

Length: 6312 bp
ATGCTGGTTCAGCTTCCGCGATTGACTAGCTCTCTGAGGGAGCCCTTCGACGTCGACCAGGCCTATTTGCAGCGCAAGCTCATCCTTCAAAACCACACCAAGCCTCGCCAATCATCGAGCTCAGTTGATGAGTCGGAGCTTGCGAGAAAGATAGTTCATAGATGGGAGGAAGCTTCGTATGAGCTGCGGCAAGCTTACAAGCAGTTTATTGGAGCTGTGGTGGAGTTGATAGATGGAGAAGTTCAATCCGAGGAGTTTCGTGAGGTGGCACTCGTTGTTTACCGGCTTTTTGGCAGACCGGAGGAGGAAGGCAGTGTTGAAACAAATTTTACCGGAAAAAAGCAGGAAGTGCAAATGCTTTTGGGGCACACAGTTTCAGATGTTAACATGCGGAAAGTTGTGTCTCTAGCTCAGAGACTTTGTGGGATGCAGTCTAGTGATCATGGGATTACACTTGTTTCAGAAAGGCCTGTAAGTGGATCTCATGACGATGCAGAATTTGGTGCCAACCTTGTTTTTCATCCTCCGGCTCGGTTCTTGGTAGATGTTTCTTTGGATGATGCGGAGTCATTTTGTGAGGAAACCGCTGGACCTTCTTCTTATTATGAAGGATCATATGGTCACAGTGGCTTGAGTGGCGGTCATTCTGCTACTGATGGGCGCAGCATCGATCTGACTTGGCTACAAGATGCATGTGATCAGATAACAAAAAGTACTACACAGCTTTCTCGAGATGAACTGGCTATGGCCATTTGTCGGGTGCTTGATTCTGACAAACCTGGTGACGAGATAGCAGGTGACTTGTTGGACCTTGTGGGGGATGGTGCATTTGAAACTGTTCAAGACCTCATATCACATCGAAAGGAACTTGTTGATGCGATCCATCATGGGTTACTAGTAATGAAATCTGACAAATCGTCTTTGACCTCTCAACCTCGCATGCCGAGTTATGGCACACAGGTTACTGTTCAAACAGAATCTGAACGGCAGATTGATAAGCTTCGGCGCAAAGAGGAAAAGCGAAATAGACGAGGAACAGAATATGGCACTGATAGTGATCTGGCTGCTGTAAATTTTTCTTCTCTACTACAAGCAAGTGAGAAAAAAAATATTTTTGATGATCTATCGGGCCTTGGTGAAGGGATAGCAGTTAATGCTCTTCCGCAAGGAACCCAGAGGAAGCACCACAAGGGGTATGAGGAGGTCCTAATTCCTCCAACACCAGGAGCACAAATGAAACCTGGAGAAAAGCTGATTGAGATCTCGGAGTTAGATGAGTTTGCTCAAGCTGCTTTTCGTGGATACAAATCTTTGAACCGCATTCAGAGCCGCATATATCATACTGTGTACTACACAAATGAGAATATACTAGTTTGTGCTCCAACAGGAGCTGGCAAAACAAACATAGCTATGATTTCCATTCTACATGAGATTGGACAGCACTTCAAAGATGGTTACCTGCACAAAGACGAGTTCAAAATAGTTTATGTTGCACCTATGAAGGCGTTAGCTGCAGAAGTCACATCAACTTTTAGCCATCGGTTATCTCCATTGAATATGACTGTGAGAGAACTTACTGGAGACATGCAACTTTCTAAGAATGAACTTGAAGAAACTCAGATGATAGTAACAACACCTGAGAAATGGGATGTCATTACTCGCAAGAGCAGTGACATGTCACTTTCAATGCTGGTGAAGCTTCTAATAATTGATGAAGTTCATCTTTTGAATGATGATAGAGGCCCGGTAATTGAGGCACTAGTTGCTCGGACTTTACGCCAGGTGGAGTCTTCACAGTCAATGATACGCATTGTGGGACTTTCCGCCACTCTACCCAACTATTTGGAGGTTGCACAGTTTCTGAGAGTGAATCCAGAGGCAGGACTCTTCTACTTTGATTCTAGCTATCGTCCAGTGCCCCTTGCACAACAGTATATTGGTATTAGTGAGACAAACTTTTCAGCCAAAACTGAATTGTTGAATGAGATATGCTACAAGAAGGTTGTGGAGTCACTAAGGCAAGGTCATCAGGCAATGGTATTTGTCCATTCTCGAAAGGACACAGCAAAAACGGCTCAGAAGCTGGTTGAACTTGCTCGCAAATTTGAAGGACTGGAGCTCTTTAAGAATGATCAGCACCCACAATTTTCATTGATCCAGAGGGAAGTTGTAAAATCCAGAAACAAAGATCTTGTTGAACTTTTTGAATTTGGAATTGGAATTCATAATGCTGGGATGTTACGTTCTGATAGAGGTCTGACTGAGCGCCTTTTCTCTGATGGACTATTGAAGGTACTTGTTTGTACAGCAACTTTGGCGTGGGGTGTAAATCTACCAGCTCACACTGTTGTGATAAAGGGGACCCAATTATATGATCCAAAGGCTGGTGGGTGGCGAGACCTGGGAATGCTTGATGTTATGCAGATATTTGGACGAGCTGGGAGACCTCAATTCGATAAAAGTGGTGAAGGTATTATAATCACGTCTCATGACAAGCTGGCCTACTATCTGCGGTTGCTGACGAGTCAACTTCCTATTGAAAGTCAGTTCATCAGTTCCTTGAAGGACAATTTAAATGCAGAGGTGGCACTTGGAACTGTCACCAATGTCAAGGAAGCTTGTGCATGGCTTGGATATACATACCTTTTTATAAGGATGAGGCTCAACCCTTTGGCATATGGTATCGCGTGGGAGGAGGTTATGGCAGATCCTTCCTTGAGTTTAAAGCAGAGATCTCTCATAGCAGATGCTGCGCGTGCCCTTGACAAAGCAAAAATGATGCGGTTTGACGAGAAAAGTGGCAATTTTTATTGTACAGAGCTTGGTCGCATAGCAAGCCACTTTTATATTCAATACTCTAGTGTTGAAACCTACAATGAAATGTTGAAACGTCACATGAATGAGACTGAGGTAATTGACATGGTAGCCCACTCCTCAGAATTTGAAAATATTGTTATTCGAGAAGAGGAACAAAATGAGCTTGAGATGTTGGTGCGAAAATTGTGTCCAGTAGAAGTTAGGGGTGGTCCTTCAAATAAACATGGAAAGATTTCCGTTCTCATTCAGGTGTACATATCTAGAGGCTCAATTGATACCTTTTCTTTGGTTTCTGATGCTCAATATATAAGTGCAAGCTTGGCACGTATTATGCGGGCCTTATTTGAGATTTGTTTGCGAAAAGGCTGGAGTGAGATGTCATTGTTCATGTTGGAATATTGCAAGGCAGTGGACCGCCAAGTTTGGCCCTATCAACATCCTTTCAGACAATTTGACAGGGAGATTTCAGCACAAATACTGCGAAATCTTGAGGAACGAGGTGCTGATCTTGATCGCCTATATGATATGGAGGAAAAAGAGATTGGGAAACTGGTCAATTATGGACCTGGAGGAAGGTTGGTTAAGCAATATTTGGGATATTTTCCATGGATCCAGTTAGCAGCAACTGTAAGTCCAATTACCAGAACTGTTTTAAAGGTGGATTTGCATATTAAACCAGACTTTATATGGAAAGATCGTTTTCACGGCACCGCCCAGCGTTGGTGGATTCTAGTTGAGGATTCTGAGAATGATCATATCTACCACTCGGAGCTATTCACTCTAACAAAACGGATGGCTAAAGGGGAACCCCAGAAGCTCTCGTTCACTGTGCCAATATTTGAGCCACATCCGCCACAGTATTATATACGTGCTGTTTCTGATTCTTGGTTGCATGCAGAGGCATTCTACACCATTTCTTTCCATAATCTAACACTACCAGAGGCCCATACTTCACATACAGAACTGCTAGATTTAAAGCCTCTCCCTGTGACATCTCTTGGAAACACTGCTTATGAAGCATTGTACAAATTCTCGCACTTTAATCCAATACAAACACAGACTTTCCATGTGCTGTATCACACAGACAATAATGTATTACTGGGAGCTCCTACTGGAAGTGGAAAAACTATTTCTGCCGAGCTTGCTATGCTTCGTCTCTTCAACACCCAGCCTGATATGAAGGTCATTTACATAGCACCTTTGAAGGCTATTGTTCGAGAAAGAATGAATGATTGGAGGAAGCGCCTTGTCTCTCAGCTAGGGAAGAAAATGGTTGAAATGACAGGAGATTATACTCCAGATTTGATGGCCATCTTGTCAGCAGATATCATAATATCAACTCCTGAAAAATGGGATGGTATCAGTCGTAATTGGCATAGCCGAACCTATGTTAAAAAGGTTGGGCTTATGATTTTGGATGAGATTCACTTGTTGGGAGCTGACCGTGGGCCTATACTCGAGGTTATTGTGTCAAGGATGAGATACATATCCTCACAGACAGAGCGTGAAGTACGGTTTGTAGGCCTTTCTACAGCTCTGGCAAATGCAGGAGATTTAGCTGATTGGTTGGGTGTTGGAGAAACTGGACTCTTCAATTTCAAACCAAGTGTCAGACCTGTACCTCTTGAAGTTCATATCCAGGGATATCCAGGAAAGTTCTATTGTCCAAGGATGAATAGCATGAACAAACCATCATATGCTGCTATATGCACCCATTCACCTACAAAACCGGTTCTAATTTTTGTTTCCTCACGTCGCCAGACAAGGCTTACTGCATTGGACCTCATTCAGTATGCAGCTTCAGATGAGCATCCAAGGCAGTTTCTTTGTATACTGGAAGAAGAACTGCAGATGGTTCTTTATCAGGTTGCTGATAGTAACCTGAGGCACACCTTGCAATTTGGCATTGGATTACACCATGCTGGTCTGAATGACAAAGATAGATCCCTGGTTGAGGAACTTTTTGCAAACAACAAGATTCAGGCATTGCTCCTTTACCCAAATACAGTATTGGTTTGTACTAGCACGTTGGCATGGGGTGTAAACCTTCCAGCGCATCTGGTTATTATTAAGGGAACAGAATTTTATGATGGGAAAACAAAAAGATACGTCGACTTCCCAATCACAGATATCTTGCAAATGATGGGTCGTGCAGGTCGCCCACAATTTGATCAACACGGGAAAGCAGTCATTCTTGTTCATGAACCCAAAAAGAGCTTCTATAAAAAGTTTTTATATGAACCATTTCCTGTCGAGAGTAGTCTGAGGGAACAGCTGCATAATCACATTAATGCGGAGATAGTTTCTGGCACAATTTGCCATAAAGAGGATGCATTGCATTATCTTACGTGGACATATTTATTCCGCAGACTGATGTTTAATCCGGCTTACTATGGCTTGGACAATACTGACGCCGAAAGTCTCAGTTCTTATTTGTCTAGGTTAGTGCAGAACACATTGGAGGATCTGGAAGACAGTGGATGCATCAAGATGAGTGAAGACAGTGTGGAGCCAATGATGCTGGGTTCGATAGCATCCCAGTATTACCTTAGCTACTTGACTGTATCGATGTTTGGTTCAAACATTGGCTCAGATACCTCTCTTGAAGTATTCTTGCATATCCTATCTGCTGCTTCTGAATATGATGAGCTTCCTGTTCGCCATAACGAGGAAAATTACAATGAGGCATTGTCTGAGAGAGTTCGATATAAAGTGGATAAGGATCGCCTAGATGATCCACATGTCAAAGCAAATCTGCTATTTCAGGCACATTTCTCGCAGTTAGAATTACCAATTACTGACTATGTCACAGACTTGAAGTCAGTTTTGGATCAAAGTATACGGATTATCCAGGCCATGATAGATATATGTGCCAACAGTGGGTGGCTTTCAAGTTCAGTTACTTGCATGCATCTTTTGCAGATGGTCATGCAGGGCTTATGGTTTGATGAGGATTCTTCCTTATGGATGTTGCCTTGCATGAATGTTGAACTTGCGGAATCACTCGGCAAACGAGGAATCTTCCATATACAGCAGCTATTGGAACTTCCCAAGGCAACTTTGCAGAACATGATTGGAAACTTCCCTGCTTCAAAATTCTTTCAGGATCTGCAGCTCTTTCCACGCATCGAAGTCAAGTTAAAAATTCTACCGAAAGATGGTGGAAAATCCTGCTCTTTAAACATCAGATTGGTGAAGACCAATTTCAGGAAATATAAATCAAGAGCATTCACTCCACGTTTTCCTAAGGTGAAAAATGAAGCATGGTGGTTGGTCCTTGGGAACACCTCTACGTTGGAACTATATGCTCTGAAGAGAGTATCTTTCTCTGATCACTTGGTTACTAACATGGAGTTGCCATCCGATTCAACCACTCTCCAGGGGATGAAACTGATTTTAATATCAGATTGTTATCTGGGGTTCGAACAAGAGCATTCAATTTCAGAACTTGAAGCCTTGAAGGATGGCAGTAACCAGAGTAGGATTATCAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DEAD Helicase_C Sec63

Protein Analysis

2103

Amino Acids

238.53

Weight (kDa)

6.04

Isoelectric Point (pI)

41.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DExH14_plug PF24557 45 - 144 1.3e-42 DExH14 plug domain
DEAD PF00270 439 - 613 1.6e-30 DEAD/DEAH box helicase
ResIII PF04851 450 - 612 3.2e-12 Type III restriction enzyme, res subunit
Helicase_C PF00271 730 - 816 3.7e-06 Helicase conserved C-terminal domain
WHD_SNRNP200 PF23445 847 - 911 1.1e-14 Winged Helix-turn-helix domain
Sec63 PF02889 938 - 1245 1e-87 Sec63 Brl domain
DEAD PF00270 1287 - 1453 2.6e-28 DEAD/DEAH box helicase
ResIII PF04851 1295 - 1420 6.7e-11 Type III restriction enzyme, res subunit
WHD_SNRNP200 PF23445 1691 - 1771 4.2e-32 Winged Helix-turn-helix domain
Sec63 PF02889 1777 - 2087 4.1e-65 Sec63 Brl domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 2477, 2651
AarI CACCTGC 1 cut(s) 785
AatII GACGTC 1 cut(s) 54
Acc36I ACCTGC 3 cut(s) 785, 1467, 4961
AccB1I GGYRCC 2 cut(s) 503, 3524
AccB7I CCANNNNNTGG 1 cut(s) 2384
AccI GTMKAC 4 cut(s) 54, 4648, 4929, 5656
AccII CGCG 3 cut(s) 19, 2687, 2755
AcoI YGGCCR 2 cut(s) 747, 4125
AcuI CTGAAG 2 cut(s) 4602, 6149
AcyI GRCGYC 2 cut(s) 51, 5229
AflIII ACRYGT 2 cut(s) 1674, 5557
AgeI ACCGGT 1 cut(s) 4546
AhdI GACNNNNNGTC 2 cut(s) 916, 5635
AhlI ACTAGT 3 cut(s) 895, 1369, 1759
AjiI CACGTC 2 cut(s) 2484, 4571
AleI CACNNNNGTG 1 cut(s) 2348
AloI GAACNNNNNNTCC 2 cut(s) 2227, 2259
Alw21I GWGCWC 5 cut(s) 122, 1231, 1381, 2121, 3925
AlwNI CAGNNNCTG 7 cut(s) 980, 1391, 1861, 2083, 2750, 3443, 5926
Ama87I CYCGRG 2 cut(s) 729, 4274
ArsI GACNNNNNNTTYG 6 cut(s) 2989, 3021, 4410, 4442, 4948, 4980
AseI ATTAAT 1 cut(s) 5106
AsiGI ACCGGT 1 cut(s) 4546
Asp700I GAANNNNTTC 3 cut(s) 837, 3049, 5061
AspLEI GCGC 7 cut(s) 75, 663, 1008, 2268, 2755, 4062, 4873
AsuC2I CCSGG 1 cut(s) 1745
AsuHPI GGTGA 9 cut(s) 794, 809, 1148, 1705, 2477, 2593, 4527, 6022, 6079
AsuII TTCGAA 1 cut(s) 6240
AvaI CYCGRG 2 cut(s) 729, 4274
AvaII GGWCC 9 cut(s) 590, 808, 1204, 2361, 3026, 3229, 3380, 4597, 6091
AxyI CCTNAGG 2 cut(s) 4700, 6063
BaeGI GKGCMC 3 cut(s) 369, 1920, 2761
BalI TGGCCA 2 cut(s) 749, 4127
BamHI GGATCC 1 cut(s) 3426
BanI GGYRCC 2 cut(s) 503, 3524
BanII GRGCYC 4 cut(s) 45, 122, 2121, 3925
BarI GAAGNNNNNNTAC 2 cut(s) 3989, 4021
BbsI GAAGAC 4 cut(s) 1782, 5295, 5322, 6020
Bbv12I GWGCWC 5 cut(s) 122, 1231, 1381, 2121, 3925
BceAI ACGGC 3 cut(s) 1001, 2088, 3538
BcgI CGANNNNNNTGC 2 cut(s) 5574, 5608
BciVI GTATCC 1 cut(s) 1295
BclI TGATCA 6 cut(s) 439, 697, 2131, 3568, 4987, 6148
BcnI CCSGG 1 cut(s) 1745
BcuI ACTAGT 3 cut(s) 895, 1369, 1759
BfmI CTRYAG 4 cut(s) 1512, 4347, 4664, 5927
BfoI RGCGCY 2 cut(s) 2269, 4063
BfuAI ACCTGC 3 cut(s) 785, 1467, 4961
BfuI GTATCC 1 cut(s) 1295
BglII AGATCT 3 cut(s) 1260, 2188, 2732
BmcAI AGTACT 1 cut(s) 715
Bme18I GGWCC 9 cut(s) 590, 808, 1204, 2361, 3026, 3229, 3380, 4597, 6091
BmeRI GACNNNNNGTC 2 cut(s) 916, 5635
BmeT110I CYCGRG 2 cut(s) 729, 4274
BmgBI CACGTC 2 cut(s) 2484, 4571
BmiI GGNNCC 9 cut(s) 42, 505, 1171, 2362, 2363, 3428, 3526, 3624, 5328
BmrI ACTGGG 2 cut(s) 3926, 5350
BmuI ACTGGG 2 cut(s) 3926, 5350
BpiI GAAGAC 4 cut(s) 1782, 5295, 5322, 6020
BpmI CTGGAG 7 cut(s) 1263, 1599, 2135, 3205, 3405, 4098, 6179
Bpu10I CCTNAGC 1 cut(s) 5366
Bpu14I TTCGAA 1 cut(s) 6240
BpuEI CTTGAG 5 cut(s) 652, 1269, 2737, 3002, 3326
BpuMI CCSGG 1 cut(s) 1745
Bsa29I ATCGAT 2 cut(s) 669, 5384
BsaAI YACGTR 3 cut(s) 3143, 3686, 5166
BsaBI GATNNNNATC 9 cut(s) 1056, 1259, 1306, 3327, 4127, 4303, 4626, 5538, 6228
BsaHI GRCGYC 2 cut(s) 51, 5229
BsaI GGTCTC 2 cut(s) 2391, 2437
BsaWI WCCGGW 3 cut(s) 298, 332, 4546
BsaXI ACNNNNNCTCC 2 cut(s) 204, 234
Bse118I RCCGGY 2 cut(s) 282, 4546
Bse21I CCTNAGG 2 cut(s) 4700, 6063
Bse3DI GCAATG 3 cut(s) 2040, 4802, 5150
Bse8I GATNNNNATC 9 cut(s) 1056, 1259, 1306, 3327, 4127, 4303, 4626, 5538, 6228
BseCI ATCGAT 2 cut(s) 669, 5384
BseJI GATNNNNATC 9 cut(s) 1056, 1259, 1306, 3327, 4127, 4303, 4626, 5538, 6228
BseMI GCAATG 3 cut(s) 2040, 4802, 5150
BseRI GAGGAG 5 cut(s) 266, 317, 1214, 2708, 2923
BseSI GKGCMC 3 cut(s) 369, 1920, 2761
BseYI CCCAGC 5 cut(s) 2231, 2438, 3531, 3984, 5338
BsgI GTGCAG 3 cut(s) 1445, 4989, 5285
Bsh1236I CGCG 3 cut(s) 19, 2687, 2755
BshNI GGYRCC 2 cut(s) 503, 3524
BshTI ACCGGT 1 cut(s) 4546
BshVI ATCGAT 2 cut(s) 669, 5384
BsiHKAI GWGCWC 5 cut(s) 122, 1231, 1381, 2121, 3925
BsiHKCI CYCGRG 2 cut(s) 729, 4274
BsiSI CCGG 7 cut(s) 283, 299, 333, 527, 1745, 4547, 5201
BslFI GGGAC 2 cut(s) 1830, 2374
BsmBI CGTCTC 2 cut(s) 2490, 3977
BsmFI GGGAC 2 cut(s) 1830, 2374
BsmI GAATGC 5 cut(s) 1320, 2412, 3719, 6044, 6250
Bso31I GGTCTC 2 cut(s) 2391, 2437
BsoBI CYCGRG 2 cut(s) 729, 4274
Bsp119I TTCGAA 1 cut(s) 6240
Bsp1286I GDGCHC 9 cut(s) 45, 122, 369, 1231, 1381, 1920, 2121, 2761, 3925
Bsp1407I TGTACA 4 cut(s) 2303, 2813, 3069, 3840
Bsp19I CCATGG 1 cut(s) 3422
BspDI ATCGAT 2 cut(s) 669, 5384
BspFNI CGCG 3 cut(s) 19, 2687, 2755
BspHI TCATGA 3 cut(s) 484, 2488, 5017
BspLI GGNNCC 9 cut(s) 42, 505, 1171, 2362, 2363, 3428, 3526, 3624, 5328
BspMAI CTGCAG 3 cut(s) 1516, 4668, 5931
BspMI ACCTGC 3 cut(s) 785, 1467, 4961
BspQI GCTCTTC 1 cut(s) 1164
BspT104I TTCGAA 1 cut(s) 6240
BspT107I GGYRCC 2 cut(s) 503, 3524
BspTNI GGTCTC 2 cut(s) 2391, 2437
BsrDI GCAATG 3 cut(s) 2040, 4802, 5150
BsrFI RCCGGY 2 cut(s) 282, 4546
BsrGI TGTACA 4 cut(s) 2303, 2813, 3069, 3840
BssAI RCCGGY 2 cut(s) 282, 4546
BssNAI GTATAC 2 cut(s) 4649, 5657
BssNI GRCGYC 2 cut(s) 51, 5229
BssT1I CCWWGG 6 cut(s) 1132, 3422, 4484, 4631, 5868, 6094
Bst1107I GTATAC 2 cut(s) 4649, 5657
Bst6I CTCTTC 6 cut(s) 1164, 1892, 2958, 3980, 4412, 6125
BstACI GRCGYC 2 cut(s) 51, 5229
BstAPI GCANNNNNTGC 7 cut(s) 349, 1154, 1763, 2750, 4711, 5133, 5734
BstAUI TGTACA 4 cut(s) 2303, 2813, 3069, 3840
BstBAI YACGTR 3 cut(s) 3143, 3686, 5166
BstBI TTCGAA 1 cut(s) 6240
BstDSI CCRYGG 2 cut(s) 3422, 4261
BstEII GGTNACC 1 cut(s) 1454
BstENI CCTNNNNNAGG 1 cut(s) 4769
BstFNI CGCG 3 cut(s) 19, 2687, 2755
BstH2I RGCGCY 2 cut(s) 2269, 4063
BstHHI GCGC 7 cut(s) 75, 663, 1008, 2268, 2755, 4062, 4873
BstNSI RCATGY 8 cut(s) 391, 696, 943, 1588, 1678, 3713, 5561, 5728
BstPI GGTNACC 1 cut(s) 1454
BstSFI CTRYAG 4 cut(s) 1512, 4347, 4664, 5927
BstSLI GKGCMC 3 cut(s) 369, 1920, 2761
BstUI CGCG 3 cut(s) 19, 2687, 2755
BstV2I GAAGAC 4 cut(s) 1782, 5295, 5322, 6020
BstX2I RGATCY 9 cut(s) 479, 1260, 2188, 2707, 2732, 3426, 4760, 5281, 5923
BstXI CCANNNNNNTGG 1 cut(s) 1843
BstYI RGATCY 9 cut(s) 479, 1260, 2188, 2707, 2732, 3426, 4760, 5281, 5923
BstZ17I GTATAC 2 cut(s) 4649, 5657
Bsu15I ATCGAT 2 cut(s) 669, 5384
Bsu36I CCTNAGG 2 cut(s) 4700, 6063
BsuI GTATCC 1 cut(s) 1295
BsuTUI ATCGAT 2 cut(s) 669, 5384
BtgI CCRYGG 2 cut(s) 3422, 4261
BtrI CACGTC 2 cut(s) 2484, 4571
BtsI GCAGTG 4 cut(s) 319, 1675, 3231, 3822
BveI ACCTGC 3 cut(s) 785, 1467, 4961
CaiI CAGNNNCTG 7 cut(s) 980, 1391, 1861, 2083, 2750, 3443, 5926
CciI TCATGA 3 cut(s) 484, 2488, 5017
CfoI GCGC 7 cut(s) 75, 663, 1008, 2268, 2755, 4062, 4873
Cfr10I RCCGGY 2 cut(s) 282, 4546
ClaI ATCGAT 2 cut(s) 669, 5384
CseI GACGC 1 cut(s) 5237
CsiI ACCWGGT 1 cut(s) 778
CspAI ACCGGT 1 cut(s) 4546
DraI TTTAAA 5 cut(s) 2574, 2724, 3471, 3792, 5997
DriI GACNNNNNGTC 2 cut(s) 916, 5635
EaeI YGGCCR 2 cut(s) 747, 4125
Eam1104I CTCTTC 6 cut(s) 1164, 1892, 2958, 3980, 4412, 6125
Eam1105I GACNNNNNGTC 2 cut(s) 916, 5635
EarI CTCTTC 6 cut(s) 1164, 1892, 2958, 3980, 4412, 6125
EciI GGCGGA 2 cut(s) 1813, 3657
Ecl136II GAGCTC 3 cut(s) 120, 2119, 3923
Eco130I CCWWGG 6 cut(s) 1132, 3422, 4484, 4631, 5868, 6094
Eco147I AGGCCT 3 cut(s) 62, 469, 4341
Eco24I GRGCYC 4 cut(s) 45, 122, 2121, 3925
Eco31I GGTCTC 2 cut(s) 2391, 2437
Eco32I GATATC 3 cut(s) 4144, 4463, 4948
Eco47I GGWCC 9 cut(s) 590, 808, 1204, 2361, 3026, 3229, 3380, 4597, 6091
Eco53kI GAGCTC 3 cut(s) 120, 2119, 3923
Eco57I CTGAAG 2 cut(s) 4602, 6149
Eco81I CCTNAGG 2 cut(s) 4700, 6063
Eco88I CYCGRG 2 cut(s) 729, 4274
Eco91I GGTNACC 1 cut(s) 1454
EcoICRI GAGCTC 3 cut(s) 120, 2119, 3923
EcoNI CCTNNNNNAGG 1 cut(s) 4769
EcoO109I RGGNCCY 2 cut(s) 1204, 2361
EcoO65I GGTNACC 1 cut(s) 1454
EcoRI GAATTC 1 cut(s) 2220
EcoRV GATATC 3 cut(s) 4144, 4463, 4948
EcoT14I CCWWGG 6 cut(s) 1132, 3422, 4484, 4631, 5868, 6094
EcoT22I ATGCAT 4 cut(s) 694, 5152, 5303, 5730
EcoT38I GRGCYC 4 cut(s) 45, 122, 2121, 3925
ErhI CCWWGG 6 cut(s) 1132, 3422, 4484, 4631, 5868, 6094
Esp3I CGTCTC 2 cut(s) 2490, 3977
FalI AAGNNNNNCTT 2 cut(s) 1707, 1739
FaqI GGGAC 2 cut(s) 1830, 2374
FauI CCCGC 1 cut(s) 3144
FauNDI CATATG 3 cut(s) 616, 2677, 4513
FbaI TGATCA 6 cut(s) 439, 697, 2131, 3568, 4987, 6148
FblI GTMKAC 4 cut(s) 54, 4648, 4929, 5656
FriOI GRGCYC 4 cut(s) 45, 122, 2121, 3925
GlaI GCGC 7 cut(s) 74, 662, 1007, 2267, 2754, 4061, 4872
GsaI CCCAGC 5 cut(s) 2235, 2442, 3535, 3988, 5342
GsuI CTGGAG 7 cut(s) 1263, 1599, 2135, 3205, 3405, 4098, 6179
HaeII RGCGCY 2 cut(s) 2269, 4063
HapII CCGG 7 cut(s) 283, 299, 333, 527, 1745, 4547, 5201
HgaI GACGC 1 cut(s) 5237
HhaI GCGC 7 cut(s) 75, 663, 1008, 2268, 2755, 4062, 4873
Hin1I GRCGYC 2 cut(s) 51, 5229
Hin6I GCGC 7 cut(s) 73, 661, 1006, 2266, 2753, 4060, 4871
HinP1I GCGC 7 cut(s) 73, 661, 1006, 2266, 2753, 4060, 4871
HincII GTYRAC 4 cut(s) 55, 385, 2528, 4930
HindII GTYRAC 4 cut(s) 55, 385, 2528, 4930
HindIII AAGCTT 6 cut(s) 171, 192, 998, 1697, 2616, 3133
HpaI GTTAAC 1 cut(s) 385
HpaII CCGG 7 cut(s) 283, 299, 333, 527, 1745, 4547, 5201
HphI GGTGA 9 cut(s) 794, 809, 1148, 1705, 2477, 2593, 4527, 6022, 6079
Hpy99I CGWCG 4 cut(s) 53, 56, 4575, 4931
Hsp92I GRCGYC 2 cut(s) 51, 5229
HspAI GCGC 7 cut(s) 73, 661, 1006, 2266, 2753, 4060, 4871
KflI GGGWCCC 1 cut(s) 2361
Ksp22I TGATCA 6 cut(s) 439, 697, 2131, 3568, 4987, 6148
KspAI GTTAAC 1 cut(s) 385
LguI GCTCTTC 1 cut(s) 1164
MabI ACCWGGT 1 cut(s) 778
MfeI CAATTG 1 cut(s) 3087
MflI RGATCY 9 cut(s) 479, 1260, 2188, 2707, 2732, 3426, 4760, 5281, 5923
MhlI GDGCHC 9 cut(s) 45, 122, 369, 1231, 1381, 1920, 2121, 2761, 3925
MlsI TGGCCA 2 cut(s) 749, 4127
MluNI TGGCCA 2 cut(s) 749, 4127
MlyI GAGTC 7 cut(s) 140, 575, 1796, 1878, 2018, 2533, 4398
MmeI TCCRAC 7 cut(s) 114, 786, 1241, 1406, 3189, 4372, 6093
Mox20I TGGCCA 2 cut(s) 749, 4127
Mph1103I ATGCAT 4 cut(s) 694, 5152, 5303, 5730
MroXI GAANNNNTTC 3 cut(s) 837, 3049, 5061
MscI TGGCCA 2 cut(s) 749, 4127
MslI CAYNNNNRTG 7 cut(s) 489, 1409, 2348, 2898, 3065, 5106, 5306
Msp20I TGGCCA 2 cut(s) 749, 4127
MspA1I CMGCKG 2 cut(s) 587, 5092
MspI CCGG 7 cut(s) 283, 299, 333, 527, 1745, 4547, 5201
MunI CAATTG 1 cut(s) 3087
Mva1269I GAATGC 5 cut(s) 1320, 2412, 3719, 6044, 6250
MvnI CGCG 3 cut(s) 19, 2687, 2755
NciI CCSGG 1 cut(s) 1745
NcoI CCATGG 1 cut(s) 3422
NdeI CATATG 3 cut(s) 616, 2677, 4513
NlaIV GGNNCC 9 cut(s) 42, 505, 1171, 2362, 2363, 3428, 3526, 3624, 5328
NmeAIII GCCGAG 3 cut(s) 969, 3979, 5802
NsiI ATGCAT 4 cut(s) 694, 5152, 5303, 5730
NspI RCATGY 8 cut(s) 391, 696, 943, 1588, 1678, 3713, 5561, 5728
NspV TTCGAA 1 cut(s) 6240
OliI CACNNNNGTG 1 cut(s) 2348
PaeI GCATGC 3 cut(s) 943, 3713, 5728
PaeR7I CTCGAG 2 cut(s) 729, 4274
PagI TCATGA 3 cut(s) 484, 2488, 5017
PaqCI CACCTGC 1 cut(s) 785
PceI AGGCCT 3 cut(s) 62, 469, 4341
PciI ACATGT 2 cut(s) 1674, 5557
PciSI GCTCTTC 1 cut(s) 1164
PcsI WCGNNNNNNNCGW 1 cut(s) 140
PctI GAATGC 5 cut(s) 1320, 2412, 3719, 6044, 6250
PdmI GAANNNNTTC 3 cut(s) 837, 3049, 5061
PflFI GACNNNGTC 1 cut(s) 5621
PflMI CCANNNNNTGG 1 cut(s) 2384
PfoI TCCNGGA 1 cut(s) 4465
PinAI ACCGGT 1 cut(s) 4546
PleI GAGTC 7 cut(s) 139, 574, 1795, 1878, 2017, 2532, 4398
PpsI GAGTC 7 cut(s) 139, 574, 1795, 1878, 2017, 2532, 4398
Ppu21I YACGTR 3 cut(s) 3143, 3686, 5166
PpuMI RGGWCCY 2 cut(s) 1204, 2361
PscI ACATGT 2 cut(s) 1674, 5557
PshBI ATTAAT 1 cut(s) 5106
PsiI TTATAA 2 cut(s) 2477, 2651
Psp124BI GAGCTC 3 cut(s) 122, 2121, 3925
Psp5II RGGWCCY 2 cut(s) 1204, 2361
PspEI GGTNACC 1 cut(s) 1454
PspFI CCCAGC 5 cut(s) 2231, 2438, 3531, 3984, 5338
PspN4I GGNNCC 9 cut(s) 42, 505, 1171, 2362, 2363, 3428, 3526, 3624, 5328
PspPPI RGGWCCY 2 cut(s) 1204, 2361
PspXI VCTCGAGB 1 cut(s) 4274
PstI CTGCAG 3 cut(s) 1516, 4668, 5931
PstNI CAGNNNCTG 7 cut(s) 980, 1391, 1861, 2083, 2750, 3443, 5926
PsuI RGATCY 9 cut(s) 479, 1260, 2188, 2707, 2732, 3426, 4760, 5281, 5923
PsyI GACNNNGTC 1 cut(s) 5621
PvuII CAGCTG 1 cut(s) 5092
RseI CAYNNNNRTG 7 cut(s) 489, 1409, 2348, 2898, 3065, 5106, 5306
SacI GAGCTC 3 cut(s) 122, 2121, 3925
SalI GTCGAC 2 cut(s) 53, 4928
SapI GCTCTTC 1 cut(s) 1164
ScaI AGTACT 1 cut(s) 715
SchI GAGTC 7 cut(s) 140, 575, 1796, 1878, 2018, 2533, 4398
SduI GDGCHC 9 cut(s) 45, 122, 369, 1231, 1381, 1920, 2121, 2761, 3925
SexAI ACCWGGT 1 cut(s) 778
SfcI CTRYAG 4 cut(s) 1512, 4347, 4664, 5927
Sfr274I CTCGAG 2 cut(s) 729, 4274
SfuI TTCGAA 1 cut(s) 6240
SinI GGWCC 9 cut(s) 590, 808, 1204, 2361, 3026, 3229, 3380, 4597, 6091
SlaI CTCGAG 2 cut(s) 729, 4274
SmiI ATTTAAAT 1 cut(s) 2574
SmiMI CAYNNNNRTG 7 cut(s) 489, 1409, 2348, 2898, 3065, 5106, 5306
SmlI CTYRAG 7 cut(s) 631, 729, 1284, 2716, 2981, 3305, 4274
SmoI CTYRAG 7 cut(s) 631, 729, 1284, 2716, 2981, 3305, 4274
SpeI ACTAGT 3 cut(s) 895, 1369, 1759
SphI GCATGC 3 cut(s) 943, 3713, 5728
SseBI AGGCCT 3 cut(s) 62, 469, 4341
SspI AATATT 5 cut(s) 1111, 2950, 3215, 3407, 3654
SstI GAGCTC 3 cut(s) 122, 2121, 3925
StuI AGGCCT 3 cut(s) 62, 469, 4341
StyI CCWWGG 6 cut(s) 1132, 3422, 4484, 4631, 5868, 6094
SwaI ATTTAAAT 1 cut(s) 2574
TatI WGTACW 7 cut(s) 713, 1347, 2303, 2813, 3069, 3840, 4835
TauI GCSGC 2 cut(s) 190, 1332
TspGWI ACGGA 3 cut(s) 3043, 3622, 5674
Tth111I GACNNNGTC 1 cut(s) 5621
Van91I CCANNNNNTGG 1 cut(s) 2384
VpaK11BI GGWCC 9 cut(s) 590, 808, 1204, 2361, 3026, 3229, 3380, 4597, 6091
VspI ATTAAT 1 cut(s) 5106
XagI CCTNNNNNAGG 1 cut(s) 4769
XbaI TCTAGA 1 cut(s) 3077
XceI RCATGY 8 cut(s) 391, 696, 943, 1588, 1678, 3713, 5561, 5728
XcmI CCANNNNNNNNNTGG 1 cut(s) 3501
XhoI CTCGAG 2 cut(s) 729, 4274
XmiI GTMKAC 4 cut(s) 54, 4648, 4929, 5656
XmnI GAANNNNTTC 3 cut(s) 837, 3049, 5061
ZraI GACGTC 1 cut(s) 52
ZrmI AGTACT 1 cut(s) 715
Zsp2I ATGCAT 4 cut(s) 694, 5152, 5303, 5730
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.