AT5G61800

endonuclease activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0015029
Physical Location & Seq
Reverse (-)
24829987 .. 24831615
1629 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G61800.1

Sequence Viewer

Length: 1500 bp
ATGATAAATTATAGTAGTTGTTCATATCTCTTGAAGCTGTGTAGAACCCTTAAACATCTGCATCAGTTTCATGCCCAATTCATCACTTCCGGCAGAATCTCTAATGATTTCAAACAAAACTCTGTTTTCGCCAATGTCCTCTTCGCCATCACCTCTATTTCACCGTCTGCGTCGGCGTCGAAGGAGGTCGTGAGCTACGCGACTTCAGTTTTCAGATTCATCACAAACCCTTCAACGTTTTGTTTCAATACGATCATTAGGATTTGTACGCTTCATGAGCCGTCGTCTCTGTCTTCTAAACGTTTCTTCGTCGAGATGCGACGTCGCTCTGTTCCTCCAGATTTCCACACTTTCCCTTTCGTATTCAAGGCTTGTGCAGCAAAGAAGAATGGTGATCTTACATTAGTCAAGACTCTGCATTGTCAAGCTTTGAGATTCGGATTGTTATCTGATTTGTTCACTTTGAACACTCTGATTCGTGTTTACTCATTGATTGCTCCAATCGATAGTGCCTTGCAACTGTTCGACGAAAATCCTCAAAGAGATGTTGTTACCTACAATGTGTTGATCGACGGGTTAGTGAAGGCACGTGAGATTGTTCGTGCAAGAGAGTTGTTTGATTCAATGCCCTTACGTGATTTGGTTTCGTGGAATAGTCTCATTTCTGGTTATGCGCAGATGAATCATTGCAGAGAAGCAATCAAACTCTTCGACGAGATGGTTGCTTTGGGTCTAAAACCCGATAATGTCGCCATTGTTTCAACTCTTTCAGCTTGTGCACAATCAGGGGATTGGCAAAAAGGGAAAGCTATTCATGATTACACGAAAAGGAAGAGACTTTTCATTGATTCCTTCTTAGCTACTGGATTGGTGGATTTCTATGCAAAATGTGGTTTCATCGATACAGCGATGGAGATCTTCGAATTATGTTCAGATAAAACTTTATTCACATGGAATGCAATGATCACTGGTCTTGCAATGCACGGTAACGGTGAGCTAACGGTTGATTACTTCCGTAAGATGGTTAGTTCTGGAATCAAACCAGACGGAGTAACCTTCATAAGTGTTTTAGTGGGTTGCAGCCATTCTGGTCTAGTTGATGAAGCCAGGAACCTTTTTGACCAAATGAGATCTTTGTATGATGTTAATAGAGAGATGAAGCATTACGGGTGTATGGCGGATTTGCTTGGAAGAGCAGGATTGATCGAAGAAGCAGCGGAAATGATCGAGCAAATGCCTAAAGATGGAGGAAACAGAGAGAAACTCTTAGCATGGAGCGGTTTGTTAGGCGGATGCAGAATCCACGGGAACATAGAGATCGCGGAGAAAGCCGCAAATCGAGTCAAGGCGTTGAGTCCCGAAGATGGAGGAGTGTATAAAGTTATGGTGGAAATGTATGCAAACGCAGAGAGATGGGAGGAAGTAGTTAAGGTTAGAGAAATAATAGACAGAGACAAGAAAGTGAAGAAGAATGTTGGATTTAGTAAAGTTTTGTCATAG

Protein Analysis

499

Amino Acids

56.05

Weight (kDa)

8.66

Isoelectric Point (pI)

39.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 181 - 210 4.9e-06 PPR repeat family
PPR_1 PF12854 181 - 209 2.7e-06 PPR repeat
PPR_2 PF13041 213 - 260 4.2e-11 PPR repeat family
PPR_1 PF12854 213 - 241 1.7e-06 PPR repeat
PPR PF01535 215 - 244 5.8e-09 PPR repeat
PPR_2 PF13041 315 - 361 8e-09 PPR repeat family
E_motif PF20431 435 - 497 6e-09 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015029)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G61800
fragaria_vesca FvH4_5g09900
malus_domestica MD00G1076000.v1.1
prunus_persica Prupe.5G139600_v2.0.a1
pyrus_communis pycom06g11970
rosa_chinensis RchiOBHm_Chr7g0188381
rosa_laevigata RLG00000004697
rosa_multiflora Rmu_co8347981.1_g000001 Rmu_sc0000952.1_g000005
rosa_roxburghii Rroxscaffold_3G00266440
rosa_rugosa Rorug06G0490300
rosa_samantha Rh7AG095000 Rh7BG096500 Rh7CG097000 Rh7CG097100
rosa_wichuraiana Rw7G008190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 325
Acc16I TGCGCA 1 cut(s) 675
AccBSI CCGCTC 1 cut(s) 1278
AccII CGCG 2 cut(s) 200, 1322
AciI CCGC 6 cut(s) 1178, 1217, 1278, 1290, 1322, 1332
AclI AACGTT 2 cut(s) 236, 301
AcuI CTGAAG 1 cut(s) 189
AcvI CACGTG 1 cut(s) 590
AcyI GRCGYC 2 cut(s) 176, 322
AfaI GTAC 1 cut(s) 268
AfiI CCNNNNNNNGG 3 cut(s) 1021, 1244, 1364
AgsI TTSAA 8 cut(s) 34, 112, 234, 247, 367, 466, 624, 762
AjnI CCWGG 1 cut(s) 1106
AjuI GAANNNNNNNTTGG 2 cut(s) 125, 157
AluBI AGCT 7 cut(s) 37, 195, 428, 773, 809, 860, 997
AluI AGCT 7 cut(s) 37, 195, 428, 773, 809, 860, 997
Alw21I GWGCWC 1 cut(s) 781
Alw26I GTCTC 4 cut(s) 291, 662, 829, 1446
Alw44I GTGCAC 1 cut(s) 777
ApaLI GTGCAC 1 cut(s) 777
ApeKI GCWGC 3 cut(s) 377, 1080, 1214
AspLEI GCGC 1 cut(s) 676
AsuHPI GGTGA 4 cut(s) 142, 153, 404, 1004
AsuII TTCGAA 1 cut(s) 921
BaeGI GKGCMC 1 cut(s) 781
BbrPI CACGTG 1 cut(s) 590
BbsI GAAGAC 1 cut(s) 285
Bbv12I GWGCWC 1 cut(s) 781
BbvI GCAGC 3 cut(s) 389, 1092, 1226
BccI CCATC 7 cut(s) 155, 712, 904, 1015, 1238, 1358, 1407
BceAI ACGGC 1 cut(s) 265
BcgI CGANNNNNNTGC 2 cut(s) 704, 738
BciT130I CCWGG 1 cut(s) 1108
BclI TGATCA 1 cut(s) 963
BcoDI GTCTC 4 cut(s) 291, 662, 829, 1446
BfaI CTAG 1 cut(s) 1094
BglII AGATCT 2 cut(s) 915, 1130
BisI GCNGC 4 cut(s) 378, 1081, 1215, 1332
BlsI GCNGC 4 cut(s) 379, 1082, 1216, 1333
Bme1390I CCNGG 1 cut(s) 1108
BmiI GGNNCC 1 cut(s) 1112
BmrFI CCNGG 1 cut(s) 1108
BmsI GCATC 3 cut(s) 70, 306, 1283
BpiI GAAGAC 1 cut(s) 285
BplI GAGNNNNNCTC 2 cut(s) 1248, 1280
BpmI CTGGAG 1 cut(s) 321
Bpu14I TTCGAA 1 cut(s) 921
Bsa29I ATCGAT 2 cut(s) 504, 900
BsaAI YACGTR 2 cut(s) 590, 635
BsaBI GATNNNNATC 2 cut(s) 445, 914
BsaHI GRCGYC 2 cut(s) 176, 322
BsaJI CCNNGG 1 cut(s) 1303
Bsc4I CCNNNNNNNGG 3 cut(s) 1021, 1244, 1364
Bse1I ACTGG 2 cut(s) 868, 973
Bse3DI GCAATG 3 cut(s) 685, 966, 984
Bse8I GATNNNNATC 2 cut(s) 445, 914
BseBI CCWGG 1 cut(s) 1108
BseCI ATCGAT 2 cut(s) 504, 900
BseDI CCNNGG 1 cut(s) 1303
BseGI GGATG 1 cut(s) 1298
BseJI GATNNNNATC 2 cut(s) 445, 914
BseLI CCNNNNNNNGG 3 cut(s) 1021, 1244, 1364
BseMI GCAATG 3 cut(s) 685, 966, 984
BseNI ACTGG 2 cut(s) 868, 973
BseRI GAGGAG 1 cut(s) 1383
BseSI GKGCMC 1 cut(s) 781
BseXI GCAGC 3 cut(s) 389, 1092, 1226
BsgI GTGCAG 1 cut(s) 396
Bsh1236I CGCG 2 cut(s) 200, 1322
BshVI ATCGAT 2 cut(s) 504, 900
BsiHKAI GWGCWC 1 cut(s) 781
BsiSI CCGG 1 cut(s) 90
BslFI GGGAC 1 cut(s) 1341
BslI CCNNNNNNNGG 3 cut(s) 1021, 1244, 1364
BsmAI GTCTC 4 cut(s) 291, 662, 829, 1446
BsmBI CGTCTC 1 cut(s) 291
BsmFI GGGAC 1 cut(s) 1341
BsmI GAATGC 1 cut(s) 961
Bsp119I TTCGAA 1 cut(s) 921
Bsp1286I GDGCHC 1 cut(s) 781
Bsp143I GATC 9 cut(s) 252, 394, 567, 915, 963, 1130, 1203, 1224, 1317
BspACI CCGC 6 cut(s) 1178, 1217, 1278, 1290, 1322, 1332
BspDI ATCGAT 2 cut(s) 504, 900
BspFNI CGCG 2 cut(s) 200, 1322
BspHI TCATGA 2 cut(s) 274, 814
BspLI GGNNCC 1 cut(s) 1112
BspQI GCTCTTC 1 cut(s) 1186
BspT104I TTCGAA 1 cut(s) 921
BsrBI CCGCTC 1 cut(s) 1278
BsrDI GCAATG 3 cut(s) 685, 966, 984
BsrI ACTGG 2 cut(s) 868, 973
BssECI CCNNGG 1 cut(s) 1303
BssMI GATC 9 cut(s) 252, 394, 567, 915, 963, 1130, 1203, 1224, 1317
BssNI GRCGYC 2 cut(s) 176, 322
Bst2UI CCWGG 1 cut(s) 1108
Bst4CI ACNGT 5 cut(s) 165, 522, 986, 992, 1003
Bst6I CTCTTC 4 cut(s) 146, 713, 827, 1186
BstACI GRCGYC 2 cut(s) 176, 322
BstBAI YACGTR 2 cut(s) 590, 635
BstBI TTCGAA 1 cut(s) 921
BstDEI CTNAG 2 cut(s) 856, 1267
BstDSI CCRYGG 1 cut(s) 1303
BstF5I GGATG 1 cut(s) 1298
BstFNI CGCG 2 cut(s) 200, 1322
BstHHI GCGC 1 cut(s) 676
BstKTI GATC 9 cut(s) 255, 397, 570, 918, 966, 1133, 1206, 1227, 1320
BstMAI GTCTC 4 cut(s) 291, 662, 829, 1446
BstMBI GATC 9 cut(s) 252, 394, 567, 915, 963, 1130, 1203, 1224, 1317
BstMWI GCNNNNNNNGC 3 cut(s) 277, 377, 1328
BstNI CCWGG 1 cut(s) 1108
BstSCI CCNGG 1 cut(s) 1106
BstSLI GKGCMC 1 cut(s) 781
BstUI CGCG 2 cut(s) 200, 1322
BstV1I GCAGC 3 cut(s) 389, 1092, 1226
BstV2I GAAGAC 1 cut(s) 285
BstX2I RGATCY 2 cut(s) 915, 1130
BstYI RGATCY 2 cut(s) 915, 1130
Bsu15I ATCGAT 2 cut(s) 504, 900
BsuTUI ATCGAT 2 cut(s) 504, 900
BtgI CCRYGG 1 cut(s) 1303
BtgZI GCGATG 1 cut(s) 923
BtsCI GGATG 1 cut(s) 1298
BtsIMutI CAGTG 1 cut(s) 966
CciI TCATGA 2 cut(s) 274, 814
CfoI GCGC 1 cut(s) 676
ClaI ATCGAT 2 cut(s) 504, 900
CseI GACGC 2 cut(s) 159, 165
Csp6I GTAC 1 cut(s) 267
CviAII CATG 5 cut(s) 71, 275, 815, 951, 1272
CviQI GTAC 1 cut(s) 267
DdeI CTNAG 2 cut(s) 856, 1267
DpnI GATC 9 cut(s) 254, 396, 569, 917, 965, 1132, 1205, 1226, 1319
DpnII GATC 9 cut(s) 252, 394, 567, 915, 963, 1130, 1203, 1224, 1317
Eam1104I CTCTTC 4 cut(s) 146, 713, 827, 1186
EarI CTCTTC 4 cut(s) 146, 713, 827, 1186
EciI GGCGGA 2 cut(s) 1193, 1305
Eco57I CTGAAG 1 cut(s) 189
Eco72I CACGTG 1 cut(s) 590
EcoRII CCWGG 1 cut(s) 1106
Esp3I CGTCTC 1 cut(s) 291
FaeI CATG 5 cut(s) 74, 278, 818, 954, 1275
FaqI GGGAC 1 cut(s) 1341
FatI CATG 5 cut(s) 70, 274, 814, 950, 1271
FbaI TGATCA 1 cut(s) 963
Fnu4HI GCNGC 4 cut(s) 378, 1081, 1215, 1332
FokI GGATG 1 cut(s) 1305
Fsp4HI GCNGC 4 cut(s) 378, 1081, 1215, 1332
FspBI CTAG 1 cut(s) 1094
FspI TGCGCA 1 cut(s) 675
GlaI GCGC 1 cut(s) 675
GluI GCNGC 4 cut(s) 378, 1081, 1215, 1332
GsuI CTGGAG 1 cut(s) 321
HapII CCGG 1 cut(s) 90
HgaI GACGC 2 cut(s) 159, 165
HhaI GCGC 1 cut(s) 676
Hin1I GRCGYC 2 cut(s) 176, 322
Hin1II CATG 5 cut(s) 74, 278, 818, 954, 1275
Hin6I GCGC 1 cut(s) 674
HinP1I GCGC 1 cut(s) 674
HindIII AAGCTT 1 cut(s) 426
HpaII CCGG 1 cut(s) 90
HphI GGTGA 4 cut(s) 142, 153, 404, 1004
Hpy166II GTNNAC 3 cut(s) 459, 484, 779
Hpy188I TCNGA 5 cut(s) 215, 440, 451, 474, 934
Hpy188III TCNNGA 9 cut(s) 31, 190, 275, 313, 338, 409, 815, 1032, 1358
Hpy8I GTNNAC 3 cut(s) 459, 484, 779
Hpy99I CGWCG 9 cut(s) 175, 181, 286, 314, 324, 327, 530, 575, 716
HpyAV CCTTC 5 cut(s) 175, 240, 577, 862, 1066
HpyCH4III ACNGT 5 cut(s) 165, 522, 986, 992, 1003
HpyCH4IV ACGT 5 cut(s) 236, 301, 322, 589, 634
HpyF10VI GCNNNNNNNGC 3 cut(s) 277, 377, 1328
HpyF3I CTNAG 2 cut(s) 856, 1267
HpySE526I ACGT 5 cut(s) 236, 301, 322, 589, 634
Hsp92I GRCGYC 2 cut(s) 176, 322
Hsp92II CATG 5 cut(s) 74, 278, 818, 954, 1275
HspAI GCGC 1 cut(s) 674
Ksp22I TGATCA 1 cut(s) 963
Kzo9I GATC 9 cut(s) 252, 394, 567, 915, 963, 1130, 1203, 1224, 1317
LguI GCTCTTC 1 cut(s) 1186
LmnI GCTCC 2 cut(s) 502, 1275
Lsp1109I GCAGC 3 cut(s) 389, 1092, 1226
LweI GCATC 3 cut(s) 70, 306, 1283
MaeI CTAG 1 cut(s) 1094
MaeII ACGT 5 cut(s) 236, 301, 322, 589, 634
MaeIII GTNAC 3 cut(s) 550, 986, 1051
MalI GATC 9 cut(s) 254, 396, 569, 917, 965, 1132, 1205, 1226, 1319
MbiI CCGCTC 1 cut(s) 1278
MboI GATC 9 cut(s) 252, 394, 567, 915, 963, 1130, 1203, 1224, 1317
MflI RGATCY 2 cut(s) 915, 1130
MhlI GDGCHC 1 cut(s) 781
MluCI AATT 3 cut(s) 7, 77, 923
MlyI GAGTC 3 cut(s) 406, 1350, 1363
MmeI TCCRAC 1 cut(s) 1456
MnlI CCTC 8 cut(s) 149, 163, 178, 345, 546, 1241, 1361, 1411
MseI TTAA 3 cut(s) 51, 1146, 1428
MspA1I CMGCKG 1 cut(s) 1217
MspI CCGG 1 cut(s) 90
MspR9I CCNGG 1 cut(s) 1108
Mva1269I GAATGC 1 cut(s) 961
MvaI CCWGG 1 cut(s) 1108
MvnI CGCG 2 cut(s) 200, 1322
MwoI GCNNNNNNNGC 3 cut(s) 277, 377, 1328
NdeII GATC 9 cut(s) 252, 394, 567, 915, 963, 1130, 1203, 1224, 1317
NlaIII CATG 5 cut(s) 74, 278, 818, 954, 1275
NlaIV GGNNCC 1 cut(s) 1112
NsbI TGCGCA 1 cut(s) 675
NspV TTCGAA 1 cut(s) 921
PagI TCATGA 2 cut(s) 274, 814
PciSI GCTCTTC 1 cut(s) 1186
PctI GAATGC 1 cut(s) 961
PfeI GAWTC 9 cut(s) 96, 216, 435, 475, 620, 682, 848, 1035, 1299
PkrI GCNGC 4 cut(s) 379, 1082, 1216, 1333
PleI GAGTC 3 cut(s) 406, 1349, 1362
PmaCI CACGTG 1 cut(s) 590
PmlI CACGTG 1 cut(s) 590
PpsI GAGTC 3 cut(s) 406, 1349, 1362
Ppu21I YACGTR 2 cut(s) 590, 635
Psp1406I AACGTT 2 cut(s) 236, 301
Psp6I CCWGG 1 cut(s) 1106
PspCI CACGTG 1 cut(s) 590
PspGI CCWGG 1 cut(s) 1106
PspN4I GGNNCC 1 cut(s) 1112
PsuI RGATCY 2 cut(s) 915, 1130
RsaI GTAC 1 cut(s) 268
RsaNI GTAC 1 cut(s) 267
SapI GCTCTTC 1 cut(s) 1186
SaqAI TTAA 3 cut(s) 51, 1146, 1428
SatI GCNGC 4 cut(s) 378, 1081, 1215, 1332
Sau3AI GATC 9 cut(s) 252, 394, 567, 915, 963, 1130, 1203, 1224, 1317
SchI GAGTC 3 cut(s) 406, 1350, 1363
ScrFI CCNGG 1 cut(s) 1108
SduI GDGCHC 1 cut(s) 781
SfaNI GCATC 3 cut(s) 70, 306, 1283
SfuI TTCGAA 1 cut(s) 921
Sse9I AATT 3 cut(s) 7, 77, 923
SsiI CCGC 6 cut(s) 1178, 1217, 1278, 1290, 1322, 1332
SspMI CTAG 1 cut(s) 1094
StyD4I CCNGG 1 cut(s) 1106
TaaI ACNGT 5 cut(s) 165, 522, 986, 992, 1003
TaiI ACGT 5 cut(s) 239, 304, 325, 592, 637
TasI AATT 3 cut(s) 7, 77, 923
TauI GCSGC 1 cut(s) 1334
TfiI GAWTC 9 cut(s) 96, 216, 435, 475, 620, 682, 848, 1035, 1299
Tru1I TTAA 3 cut(s) 51, 1146, 1428
Tru9I TTAA 3 cut(s) 51, 1146, 1428
TscAI CASTG 1 cut(s) 973
TseI GCWGC 3 cut(s) 377, 1080, 1214
TspGWI ACGGA 2 cut(s) 1004, 1062
TspRI CASTG 1 cut(s) 973
VneI GTGCAC 1 cut(s) 777
XspI CTAG 1 cut(s) 1094
ZraI GACGTC 1 cut(s) 323
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.