AT5G62260

Domain of unknown function (DUF296)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0012862
Physical Location & Seq
Forward (+)
25008843 .. 25011666
2824 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G62260.2

Sequence Viewer

Length: 1215 bp
ATGGAGGAGAAAGGTGAAATAAGTCCAAGTGGGGTCGTCACAGTTAAGGGAGATGAAGCTTTGGTTCCAAGAACAGAGTTTCAACAAAACCCTAGTTTTCTCCAATTCGTGAGTCCCACAACGGTAGTGACTCCTCTCCCTCCTCCACCAGCTCCGTCTTCGGCTCCGGTACCAACCACCGTGACTCCTGGCTCAGCCACAGCATCAACCGGATCAGATCCGACGAAAAAGAAGAGAGGAAGACCGAGGAAGTACGCACCAGACGGAAGCCTGAACCCTAGGTTTTTGCGACCAACTCTGTCTCCAACTCCAATCTCATCTTCGATTCCATTGTCTGGAGATTATCAATGGAAACGAGGCAAAGCTCAGCAGCAGCATCAGCCTCTTGAGTTCGTCAAGAAATCTCACAAATTCGAGTATGGAAGCCCAGCTCCTACTCCTCCTCTTCCTGGGCTCTCATGCTATGTGGGTGCAAATTTTACGACCCATCAATTTACTGTCAATGGCGGTGAGGATGTGACAATGAAGGTTATGCCGTATTCGCAACAAGGATCTCGAGCTATCTGCATTCTTTCTGCAACTGGTTCGATCTCTAATGTCACTCTTGGTCAACCTACCAATGCAGGTGGCACTCTTACATATGAGGGTCGGTTTGAGATACTATCGCTATCTGGTTCCTTTATGCCTACTGAAAATGGAGGAACAAAAGGTCGGGCTGGTGGGATGAGCATTTCTTTAGCCGGACCAAATGGCAATATATTTGGTGGTGGCCTTGCCGGTATGCTTATAGCAGCTGGTCCTGTTCAGGTGGTAATGGGAAGTTTCATTGTGATGCATCAAGCAGAACAAAATCAGAAGAAGAAACCTAGAGTTATGGAGGCTTTTGCTCCACCACAACCACAAGCACCACCACAACTGCAACAACAACAACCTCCTACTTTTACCATAACAACCGTGAATTCAACTTCTCCTTCGGTGAACACCGTAGAGGAGCAGAAACCACAAGCTTATGGTGGTGGTATAGTGAGACCGATGGCTCAAATGCCTTCTTCTTTCCAAAACGACAATTCAACTATGAACAATTTCACACCGGCCTATCATGGATACGGGAATATGAACACTGGTACTACTCACAAAGAAGAACATGAAGACGAAGATGGTGGTGATGATGATGATGATTCAGGCGATACTAGGAGCCAATCTCATAGTGGTTGA

Protein Analysis

404

Amino Acids

42.8

Weight (kDa)

6.46

Isoelectric Point (pI)

54.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PCC PF03479 162 - 270 1.3e-21 Plants and Prokaryotes Conserved (PCC) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0012862)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G62260 AT5G62260 AT5G62260
fragaria_vesca FvH4_7g28180
malus_domestica MD01G1185700.v1.1 MD07G1257800.v1.1
prunus_persica Prupe.2G282300_v2.0.a1
pyrus_communis pycom07g23090
rosa_chinensis RchiOBHm_Chr1g0375131
rosa_laevigata RLG00000026684
rosa_multiflora Rmu_sc0000554.1_g000021
rosa_roxburghii Rroxscaffold_4G00282990
rosa_rugosa Rorug01G0388700
rosa_samantha Rh1AG399200 Rh1BG365300 Rh1CG378200 Rh1DG396000
rosa_wichuraiana Rw1G035440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 614
Acc36I ACCTGC 1 cut(s) 614
Acc65I GGTACC 1 cut(s) 169
AccB1I GGYRCC 1 cut(s) 169
AccB7I CCANNNNNTGG 1 cut(s) 335
AciI CCGC 1 cut(s) 507
AclWI GGATC 3 cut(s) 212, 220, 559
AcsI RAATTY 3 cut(s) 410, 475, 958
AfaI GTAC 3 cut(s) 171, 254, 1126
AfiI CCNNNNNNNGG 2 cut(s) 335, 449
AgsI TTSAA 3 cut(s) 83, 963, 1071
AjnI CCWGG 2 cut(s) 187, 448
AjuI GAANNNNNNNTTGG 2 cut(s) 304, 336
AluBI AGCT 7 cut(s) 59, 152, 365, 431, 560, 794, 1007
AluI AGCT 7 cut(s) 59, 152, 365, 431, 560, 794, 1007
Alw26I GTCTC 2 cut(s) 306, 1021
AlwI GGATC 3 cut(s) 212, 220, 559
Ama87I CYCGRG 1 cut(s) 555
AoxI GGCC 2 cut(s) 769, 1092
ApeKI GCWGC 3 cut(s) 370, 373, 791
ApoI RAATTY 3 cut(s) 410, 475, 958
Asp700I GAANNNNTTC 1 cut(s) 1082
Asp718I GGTACC 1 cut(s) 169
AspA2I CCTAGG 1 cut(s) 278
AspS9I GGNCC 2 cut(s) 743, 797
AsuHPI GGTGA 4 cut(s) 26, 521, 988, 1175
AvaI CYCGRG 1 cut(s) 555
AvaII GGWCC 2 cut(s) 743, 797
AvrII CCTAGG 1 cut(s) 278
BanI GGYRCC 1 cut(s) 169
BanII GRGCYC 1 cut(s) 456
BbsI GAAGAC 3 cut(s) 150, 247, 1155
BbvI GCAGC 3 cut(s) 382, 385, 803
BccI CCATC 3 cut(s) 495, 1027, 1151
BceAI ACGGC 1 cut(s) 520
BcgI CGANNNNNNTGC 4 cut(s) 546, 567, 580, 601
BciT130I CCWGG 2 cut(s) 189, 450
BciVI GTATCC 1 cut(s) 1097
BcoDI GTCTC 2 cut(s) 306, 1021
BfaI CTAG 4 cut(s) 93, 279, 867, 1191
BfuAI ACCTGC 1 cut(s) 614
BfuI GTATCC 1 cut(s) 1097
BisI GCNGC 3 cut(s) 371, 374, 792
BlnI CCTAGG 1 cut(s) 278
BlpI GCTNAGC 2 cut(s) 193, 366
BlsI GCNGC 3 cut(s) 372, 375, 793
Bme1390I CCNGG 2 cut(s) 189, 450
Bme18I GGWCC 2 cut(s) 743, 797
BmeT110I CYCGRG 1 cut(s) 555
BmgT120I GGNCC 2 cut(s) 743, 797
BmiI GGNNCC 5 cut(s) 66, 165, 171, 676, 1196
BmrFI CCNGG 2 cut(s) 189, 450
BmsI GCATC 4 cut(s) 212, 385, 822, 844
BpiI GAAGAC 3 cut(s) 150, 247, 1155
BplI GAGNNNNNCTC 1 cut(s) 1186
BpmI CTGGAG 1 cut(s) 357
Bpu1102I GCTNAGC 2 cut(s) 193, 366
BpuEI CTTGAG 1 cut(s) 407
BsaI GGTCTC 1 cut(s) 1021
BsaJI CCNNGG 3 cut(s) 245, 278, 449
BsaWI WCCGGW 2 cut(s) 166, 209
BsaXI ACNNNNNCTCC 4 cut(s) 169, 199, 286, 316
Bsc4I CCNNNNNNNGG 2 cut(s) 335, 449
Bse118I RCCGGY 2 cut(s) 776, 1090
Bse1I ACTGG 2 cut(s) 586, 1126
BseBI CCWGG 2 cut(s) 189, 450
BseDI CCNNGG 3 cut(s) 245, 278, 449
BseGI GGATG 2 cut(s) 520, 729
BseLI CCNNNNNNNGG 2 cut(s) 335, 449
BseMII CTCAG 2 cut(s) 207, 380
BseNI ACTGG 2 cut(s) 586, 1126
BseRI GAGGAG 6 cut(s) 20, 123, 132, 429, 432, 1004
BseXI GCAGC 3 cut(s) 382, 385, 803
BseYI CCCAGC 1 cut(s) 427
BshFI GGCC 2 cut(s) 771, 1094
BshNI GGYRCC 1 cut(s) 169
BsiHKCI CYCGRG 1 cut(s) 555
BsiSI CCGG 5 cut(s) 167, 210, 741, 777, 1091
BslFI GGGAC 1 cut(s) 99
BslI CCNNNNNNNGG 2 cut(s) 335, 449
BsmAI GTCTC 2 cut(s) 306, 1021
BsmFI GGGAC 1 cut(s) 99
BsmI GAATGC 1 cut(s) 567
BsnI GGCC 2 cut(s) 771, 1094
Bso31I GGTCTC 1 cut(s) 1021
BsoBI CYCGRG 1 cut(s) 555
Bsp1286I GDGCHC 1 cut(s) 456
Bsp143I GATC 4 cut(s) 212, 217, 551, 588
Bsp1720I GCTNAGC 2 cut(s) 193, 366
BspACI CCGC 1 cut(s) 507
BspANI GGCC 2 cut(s) 771, 1094
BspCNI CTCAG 2 cut(s) 206, 379
BspLI GGNNCC 5 cut(s) 66, 165, 171, 676, 1196
BspMI ACCTGC 1 cut(s) 614
BspPI GGATC 3 cut(s) 212, 220, 559
BspT107I GGYRCC 1 cut(s) 169
BspTNI GGTCTC 1 cut(s) 1021
BsrFI RCCGGY 2 cut(s) 776, 1090
BsrI ACTGG 2 cut(s) 586, 1126
BssAI RCCGGY 2 cut(s) 776, 1090
BssECI CCNNGG 3 cut(s) 245, 278, 449
BssMI GATC 4 cut(s) 212, 217, 551, 588
BssT1I CCWWGG 1 cut(s) 278
Bst2UI CCWGG 2 cut(s) 189, 450
Bst4CI ACNGT 6 cut(s) 43, 124, 181, 499, 955, 985
Bst6I CTCTTC 2 cut(s) 227, 450
BstDEI CTNAG 2 cut(s) 193, 366
BstF5I GGATG 2 cut(s) 520, 729
BstKTI GATC 4 cut(s) 215, 220, 554, 591
BstMAI GTCTC 2 cut(s) 306, 1021
BstMBI GATC 4 cut(s) 212, 217, 551, 588
BstMWI GCNNNNNNNGC 2 cut(s) 379, 541
BstNI CCWGG 2 cut(s) 189, 450
BstSCI CCNGG 2 cut(s) 187, 448
BstV1I GCAGC 3 cut(s) 382, 385, 803
BstV2I GAAGAC 3 cut(s) 150, 247, 1155
BstX2I RGATCY 2 cut(s) 217, 551
BstYI RGATCY 2 cut(s) 217, 551
BsuI GTATCC 1 cut(s) 1097
BsuRI GGCC 2 cut(s) 771, 1094
BtsCI GGATG 2 cut(s) 520, 729
BtsIMutI CAGTG 1 cut(s) 1119
BveI ACCTGC 1 cut(s) 614
Cfr10I RCCGGY 2 cut(s) 776, 1090
Cfr13I GGNCC 2 cut(s) 743, 797
Csp6I GTAC 3 cut(s) 170, 253, 1125
CspCI CAANNNNNGTGG 2 cut(s) 607, 642
CviAII CATG 3 cut(s) 459, 1100, 1145
CviQI GTAC 3 cut(s) 170, 253, 1125
DdeI CTNAG 2 cut(s) 193, 366
DpnI GATC 4 cut(s) 214, 219, 553, 590
DpnII GATC 4 cut(s) 212, 217, 551, 588
Eam1104I CTCTTC 2 cut(s) 227, 450
EarI CTCTTC 2 cut(s) 227, 450
Eco130I CCWWGG 1 cut(s) 278
Eco24I GRGCYC 1 cut(s) 456
Eco31I GGTCTC 1 cut(s) 1021
Eco47I GGWCC 2 cut(s) 743, 797
Eco88I CYCGRG 1 cut(s) 555
EcoRI GAATTC 1 cut(s) 958
EcoRII CCWGG 2 cut(s) 187, 448
EcoT14I CCWWGG 1 cut(s) 278
EcoT22I ATGCAT 1 cut(s) 837
EcoT38I GRGCYC 1 cut(s) 456
ErhI CCWWGG 1 cut(s) 278
FaeI CATG 3 cut(s) 462, 1103, 1148
FaqI GGGAC 1 cut(s) 99
FatI CATG 3 cut(s) 458, 1099, 1144
FauNDI CATATG 1 cut(s) 640
Fnu4HI GCNGC 3 cut(s) 371, 374, 792
FokI GGATG 2 cut(s) 527, 736
FriOI GRGCYC 1 cut(s) 456
Fsp4HI GCNGC 3 cut(s) 371, 374, 792
FspBI CTAG 4 cut(s) 93, 279, 867, 1191
GluI GCNGC 3 cut(s) 371, 374, 792
GsaI CCCAGC 1 cut(s) 431
GsuI CTGGAG 1 cut(s) 357
HaeIII GGCC 2 cut(s) 771, 1094
HapII CCGG 5 cut(s) 167, 210, 741, 777, 1091
Hin1II CATG 3 cut(s) 462, 1103, 1148
HincII GTYRAC 1 cut(s) 611
HindII GTYRAC 1 cut(s) 611
HindIII AAGCTT 2 cut(s) 57, 1005
HinfI GANTC 5 cut(s) 112, 130, 184, 325, 1178
HpaII CCGG 5 cut(s) 167, 210, 741, 777, 1091
HphI GGTGA 4 cut(s) 26, 521, 988, 1175
Hpy166II GTNNAC 2 cut(s) 611, 979
Hpy188I TCNGA 3 cut(s) 217, 222, 855
Hpy188III TCNNGA 5 cut(s) 109, 336, 386, 397, 555
Hpy8I GTNNAC 2 cut(s) 611, 979
Hpy99I CGWCG 1 cut(s) 226
HpyAV CCTTC 3 cut(s) 520, 981, 1056
HpyCH4III ACNGT 6 cut(s) 43, 124, 181, 499, 955, 985
HpyCH4V TGCA 6 cut(s) 473, 567, 578, 623, 835, 919
HpyF10VI GCNNNNNNNGC 2 cut(s) 379, 541
HpyF3I CTNAG 2 cut(s) 193, 366
Hsp92II CATG 3 cut(s) 462, 1103, 1148
KpnI GGTACC 1 cut(s) 173
Kzo9I GATC 4 cut(s) 212, 217, 551, 588
LmnI GCTCC 6 cut(s) 157, 169, 436, 892, 991, 1194
Lsp1109I GCAGC 3 cut(s) 382, 385, 803
LweI GCATC 4 cut(s) 212, 385, 822, 844
MaeI CTAG 4 cut(s) 93, 279, 867, 1191
MaeIII GTNAC 5 cut(s) 37, 127, 181, 517, 598
MalI GATC 4 cut(s) 214, 219, 553, 590
MboI GATC 4 cut(s) 212, 217, 551, 588
MflI RGATCY 2 cut(s) 217, 551
MhlI GDGCHC 1 cut(s) 456
MluCI AATT 7 cut(s) 104, 410, 475, 491, 958, 1066, 1081
MlyI GAGTC 3 cut(s) 121, 124, 178
MmeI TCCRAC 2 cut(s) 245, 329
Mph1103I ATGCAT 1 cut(s) 837
MroXI GAANNNNTTC 1 cut(s) 1082
MseI TTAA 1 cut(s) 45
MslI CAYNNNNRTG 1 cut(s) 830
MspA1I CMGCKG 1 cut(s) 794
MspI CCGG 5 cut(s) 167, 210, 741, 777, 1091
MspR9I CCNGG 2 cut(s) 189, 450
Mva1269I GAATGC 1 cut(s) 567
MvaI CCWGG 2 cut(s) 189, 450
MwoI GCNNNNNNNGC 2 cut(s) 379, 541
NdeI CATATG 1 cut(s) 640
NdeII GATC 4 cut(s) 212, 217, 551, 588
NlaIII CATG 3 cut(s) 462, 1103, 1148
NlaIV GGNNCC 5 cut(s) 66, 165, 171, 676, 1196
NmuCI GTSAC 5 cut(s) 37, 127, 181, 517, 598
NsiI ATGCAT 1 cut(s) 837
PaeR7I CTCGAG 1 cut(s) 555
PaqCI CACCTGC 1 cut(s) 614
PctI GAATGC 1 cut(s) 567
PdmI GAANNNNTTC 1 cut(s) 1082
PfeI GAWTC 2 cut(s) 325, 1178
PflMI CCANNNNNTGG 1 cut(s) 335
PkrI GCNGC 3 cut(s) 372, 375, 793
PleI GAGTC 3 cut(s) 120, 124, 178
PpsI GAGTC 3 cut(s) 120, 124, 178
Psp6I CCWGG 2 cut(s) 187, 448
PspFI CCCAGC 1 cut(s) 427
PspGI CCWGG 2 cut(s) 187, 448
PspN4I GGNNCC 5 cut(s) 66, 165, 171, 676, 1196
PspPI GGNCC 2 cut(s) 743, 797
PsuI RGATCY 2 cut(s) 217, 551
PvuII CAGCTG 1 cut(s) 794
RsaI GTAC 3 cut(s) 171, 254, 1126
RsaNI GTAC 3 cut(s) 170, 253, 1125
RseI CAYNNNNRTG 1 cut(s) 830
SaqAI TTAA 1 cut(s) 45
SatI GCNGC 3 cut(s) 371, 374, 792
Sau3AI GATC 4 cut(s) 212, 217, 551, 588
Sau96I GGNCC 2 cut(s) 743, 797
SchI GAGTC 3 cut(s) 121, 124, 178
ScrFI CCNGG 2 cut(s) 189, 450
SduI GDGCHC 1 cut(s) 456
SfaNI GCATC 4 cut(s) 212, 385, 822, 844
Sfr274I CTCGAG 1 cut(s) 555
SinI GGWCC 2 cut(s) 743, 797
SlaI CTCGAG 1 cut(s) 555
SmiMI CAYNNNNRTG 1 cut(s) 830
SmlI CTYRAG 2 cut(s) 386, 555
SmoI CTYRAG 2 cut(s) 386, 555
Sse9I AATT 7 cut(s) 104, 410, 475, 491, 958, 1066, 1081
SsiI CCGC 1 cut(s) 507
SspMI CTAG 4 cut(s) 93, 279, 867, 1191
StyD4I CCNGG 2 cut(s) 187, 448
StyI CCWWGG 1 cut(s) 278
TaaI ACNGT 6 cut(s) 43, 124, 181, 499, 955, 985
TaqI TCGA 4 cut(s) 323, 414, 556, 587
TaqII GACCGA 2 cut(s) 259, 1045
TasI AATT 7 cut(s) 104, 410, 475, 491, 958, 1066, 1081
TfiI GAWTC 2 cut(s) 325, 1178
Tru1I TTAA 1 cut(s) 45
Tru9I TTAA 1 cut(s) 45
TscAI CASTG 1 cut(s) 1126
TseFI GTSAC 5 cut(s) 37, 127, 181, 517, 598
TseI GCWGC 3 cut(s) 370, 373, 791
Tsp45I GTSAC 5 cut(s) 37, 127, 181, 517, 598
TspDTI ATGAA 6 cut(s) 69, 539, 814, 1091, 1130, 1161
TspGWI ACGGA 2 cut(s) 144, 279
TspRI CASTG 1 cut(s) 1126
Van91I CCANNNNNTGG 1 cut(s) 335
VpaK11BI GGWCC 2 cut(s) 743, 797
XapI RAATTY 3 cut(s) 410, 475, 958
XcmI CCANNNNNNNNNTGG 1 cut(s) 1205
XhoI CTCGAG 1 cut(s) 555
XmaJI CCTAGG 1 cut(s) 278
XmnI GAANNNNTTC 1 cut(s) 1082
XspI CTAG 4 cut(s) 93, 279, 867, 1191
Zsp2I ATGCAT 1 cut(s) 837
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.