MD01G1185700.v1.1

AT-hook motif nuclear-localized protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0012862
Physical Location & Seq
Reverse (-)
28465009 .. 28468921
3913 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1185700.v1.1.491

Sequence Viewer

Length: 1029 bp
ATGCAAATGGAGGAGAAAGACAACAAGTTGGTTTCTGGTGTAGCAGTGAGCGGTGAACAAGCTCCAGAGAATTACAGAATTGCCCCCAGAACCGAAAACCCTAGCCCATCTGGGGGCCCCACAACGGTGGTAGCTCCGATGACGTCGCCGATGAGCTTGACTGGGACCGGTACGGATGGGAAGAAGAAGAGGGGCAGGCCTAGGAAGTACGGACCCGACAGCAAGCCCGTCCGGTCGTCGGCATTGTCCCCGATGCCGATTTCGTCGTCGATTCTGCTCCCTGGAGAGTTCTCCGCCTGGAAGAGGGGCAGAGGCAAGCCCGTTGACTCGATCAAGAAGTCTCACAAGCATGACGTGTTTGAGAACTCAGGTGACAAAATTGCATACTCAGTCGGTGCGAACTTTACACCTCACGTCCTCAATGTTCATTCGGGGGAGGATGTTACAATGAAGATCATGTCATTCTCTCAACAAGGAGCTCGAGCTATATGCATACTTTCTGCAAATGGTACCATTTCAAATGTTACGCTTAGGCAGGCCAGTTCTTCCGGGGGTACTTTAACATATGAGGGCCGCTTTGAGATACTCTCCTTGTCGGGATCTTATATACCAACTGAGAATGCAGGAACAAAGAGCAGATCCGGTGGCATGAGTGTTGCTTTGGCAGGCCCAGACGGTAGAGTAGTTGGGGGTGGACTTGCTGGTATGCTGATCGCTGCTGGCCCCGTGCAGGTTGTGGTGGGCAGTTTTCTGCAAGGTCACCAGCAGGAACAGAAGCCAAAGAAGCAGAGAGTCGAGCCTGTATCATCGTCAATTGTCCCTATCATTGTCAATGCTATGTCTGGTGAAGAAATGAAGGTCTATGGCGGAGTAAAGCCCATTCTGACGGCTCCTTCCTTCCACGGAGACCAATCAAATTCTCCGAATCCCGTGCACGGCTTCAAGAACTCAGCTTCTGAGAGTAAATCATTGTTGTCTGAAGATGATTCTAAAGGCTCTGGTCAAGCAAACTGTGAAGTTTCTTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF296

Protein Analysis

343

Amino Acids

35.81

Weight (kDa)

9.36

Isoelectric Point (pI)

46.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PCC PF03479 136 - 250 5.7e-27 Plants and Prokaryotes Conserved (PCC) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012862)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G62260 AT5G62260 AT5G62260
fragaria_vesca FvH4_7g28180
malus_domestica MD01G1185700.v1.1 MD07G1257800.v1.1
prunus_persica Prupe.2G282300_v2.0.a1
pyrus_communis pycom07g23090
rosa_chinensis RchiOBHm_Chr1g0375131
rosa_laevigata RLG00000026684
rosa_multiflora Rmu_sc0000554.1_g000021
rosa_roxburghii Rroxscaffold_4G00282990
rosa_rugosa Rorug01G0388700
rosa_samantha Rh1AG399200 Rh1BG365300 Rh1CG378200 Rh1DG396000
rosa_wichuraiana Rw1G035440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 146
Acc36I ACCTGC 1 cut(s) 721
Acc65I GGTACC 1 cut(s) 509
AccB1I GGYRCC 1 cut(s) 509
AccBSI CCGCTC 1 cut(s) 51
AciI CCGC 4 cut(s) 51, 294, 574, 867
AclWI GGATC 2 cut(s) 607, 633
AcsI RAATTY 1 cut(s) 916
AcuI CTGAAG 1 cut(s) 999
AcyI GRCGYC 1 cut(s) 143
AfaI GTAC 4 cut(s) 172, 209, 511, 556
AfiI CCNNNNNNNGG 7 cut(s) 112, 113, 124, 238, 303, 730, 935
AflIII ACRYGT 1 cut(s) 354
AgeI ACCGGT 1 cut(s) 167
AgsI TTSAA 2 cut(s) 519, 943
AjiI CACGTC 2 cut(s) 355, 415
AjnI CCWGG 2 cut(s) 280, 296
AleI CACNNNNGTG 1 cut(s) 125
AluBI AGCT 6 cut(s) 62, 134, 156, 479, 485, 953
AluI AGCT 6 cut(s) 62, 134, 156, 479, 485, 953
Alw21I GWGCWC 2 cut(s) 481, 936
Alw26I GTCTC 2 cut(s) 345, 900
Alw44I GTGCAC 1 cut(s) 932
AlwI GGATC 2 cut(s) 607, 633
AlwNI CAGNNNCTG 1 cut(s) 956
Ama87I CYCGRG 1 cut(s) 480
AoxI GGCC 6 cut(s) 115, 197, 537, 571, 667, 721
ApaI GGGCCC 1 cut(s) 119
ApaLI GTGCAC 1 cut(s) 932
ApeKI GCWGC 1 cut(s) 716
ApoI RAATTY 1 cut(s) 916
AsiGI ACCGGT 1 cut(s) 167
Asp718I GGTACC 1 cut(s) 509
AspA2I CCTAGG 1 cut(s) 200
AspS9I GGNCC 7 cut(s) 115, 116, 165, 212, 571, 668, 722
AsuC2I CCSGG 1 cut(s) 550
AsuHPI GGTGA 4 cut(s) 65, 383, 752, 857
AvaI CYCGRG 1 cut(s) 480
AvaII GGWCC 2 cut(s) 165, 212
AvrII CCTAGG 1 cut(s) 200
BaeGI GKGCMC 2 cut(s) 119, 936
BanI GGYRCC 1 cut(s) 509
BanII GRGCYC 2 cut(s) 119, 481
Bbv12I GWGCWC 2 cut(s) 481, 936
BbvI GCAGC 1 cut(s) 703
BccI CCATC 2 cut(s) 115, 170
BceAI ACGGC 2 cut(s) 903, 952
BcgI CGANNNNNNTGC 4 cut(s) 471, 505, 913, 947
BciT130I CCWGG 2 cut(s) 282, 298
BcnI CCSGG 1 cut(s) 550
BcoDI GTCTC 2 cut(s) 345, 900
BfaI CTAG 2 cut(s) 102, 201
BfuAI ACCTGC 1 cut(s) 721
BisI GCNGC 2 cut(s) 574, 717
BlnI CCTAGG 1 cut(s) 200
BlsI GCNGC 2 cut(s) 575, 718
Bme1390I CCNGG 3 cut(s) 282, 298, 550
Bme18I GGWCC 2 cut(s) 165, 212
BmeT110I CYCGRG 1 cut(s) 480
BmgBI CACGTC 2 cut(s) 355, 415
BmgT120I GGNCC 7 cut(s) 115, 116, 165, 212, 571, 668, 722
BmiI GGNNCC 8 cut(s) 116, 117, 118, 166, 214, 511, 724, 891
BmrFI CCNGG 3 cut(s) 282, 298, 550
BmrI ACTGGG 1 cut(s) 171
BmsI GCATC 1 cut(s) 243
BmuI ACTGGG 1 cut(s) 171
BplI GAGNNNNNCTC 2 cut(s) 572, 604
BpmI CTGGAG 2 cut(s) 48, 303
Bpu10I CCTNAGC 1 cut(s) 530
BpuMI CCSGG 1 cut(s) 550
BsaHI GRCGYC 1 cut(s) 143
BsaI GGTCTC 1 cut(s) 900
BsaJI CCNNGG 4 cut(s) 200, 280, 549, 901
BsaWI WCCGGW 3 cut(s) 167, 231, 641
Bsc4I CCNNNNNNNGG 7 cut(s) 112, 113, 124, 238, 303, 730, 935
Bse118I RCCGGY 1 cut(s) 167
Bse1I ACTGG 2 cut(s) 166, 540
BseBI CCWGG 2 cut(s) 282, 298
BseDI CCNNGG 4 cut(s) 200, 280, 549, 901
BseGI GGATG 2 cut(s) 181, 445
BseLI CCNNNNNNNGG 7 cut(s) 112, 113, 124, 238, 303, 730, 935
BseMII CTCAG 5 cut(s) 381, 402, 606, 948, 963
BseNI ACTGG 2 cut(s) 166, 540
BseRI GAGGAG 1 cut(s) 26
BseSI GKGCMC 2 cut(s) 119, 936
BseXI GCAGC 1 cut(s) 703
BsgI GTGCAG 1 cut(s) 749
Bsh1285I CGRYCG 1 cut(s) 236
BshFI GGCC 6 cut(s) 117, 199, 539, 573, 669, 723
BshNI GGYRCC 1 cut(s) 509
BshTI ACCGGT 1 cut(s) 167
BsiEI CGRYCG 1 cut(s) 236
BsiHKAI GWGCWC 2 cut(s) 481, 936
BsiHKCI CYCGRG 1 cut(s) 480
BsiSI CCGG 4 cut(s) 168, 232, 549, 642
BslFI GGGAC 3 cut(s) 178, 232, 803
BslI CCNNNNNNNGG 7 cut(s) 112, 113, 124, 238, 303, 730, 935
BsmAI GTCTC 2 cut(s) 345, 900
BsmFI GGGAC 3 cut(s) 178, 232, 803
BsmI GAATGC 1 cut(s) 625
BsnI GGCC 6 cut(s) 117, 199, 539, 573, 669, 723
Bso31I GGTCTC 1 cut(s) 900
BsoBI CYCGRG 1 cut(s) 480
Bsp120I GGGCCC 1 cut(s) 115
Bsp1286I GDGCHC 3 cut(s) 119, 481, 936
Bsp143I GATC 5 cut(s) 330, 453, 599, 638, 711
BspACI CCGC 4 cut(s) 51, 294, 574, 867
BspANI GGCC 6 cut(s) 117, 199, 539, 573, 669, 723
BspCNI CTCAG 5 cut(s) 380, 401, 607, 949, 962
BspLI GGNNCC 8 cut(s) 116, 117, 118, 166, 214, 511, 724, 891
BspMI ACCTGC 1 cut(s) 721
BspPI GGATC 2 cut(s) 607, 633
BspT107I GGYRCC 1 cut(s) 509
BspTNI GGTCTC 1 cut(s) 900
BsrBI CCGCTC 1 cut(s) 51
BsrFI RCCGGY 1 cut(s) 167
BsrI ACTGG 2 cut(s) 166, 540
BssAI RCCGGY 1 cut(s) 167
BssECI CCNNGG 4 cut(s) 200, 280, 549, 901
BssMI GATC 5 cut(s) 330, 453, 599, 638, 711
BssNI GRCGYC 1 cut(s) 143
BssT1I CCWWGG 1 cut(s) 200
Bst2UI CCWGG 2 cut(s) 282, 298
Bst4CI ACNGT 3 cut(s) 127, 677, 1013
Bst6I CTCTTC 2 cut(s) 182, 296
BstACI GRCGYC 1 cut(s) 143
BstC8I GCNNGC 6 cut(s) 197, 224, 317, 537, 667, 721
BstDEI CTNAG 6 cut(s) 367, 388, 530, 615, 949, 957
BstDSI CCRYGG 1 cut(s) 901
BstEII GGTNACC 1 cut(s) 758
BstENI CCTNNNNNAGG 1 cut(s) 301
BstF5I GGATG 2 cut(s) 181, 445
BstKTI GATC 5 cut(s) 333, 456, 602, 641, 714
BstMAI GTCTC 2 cut(s) 345, 900
BstMBI GATC 5 cut(s) 330, 453, 599, 638, 711
BstMCI CGRYCG 1 cut(s) 236
BstMWI GCNNNNNNNGC 1 cut(s) 784
BstNI CCWGG 2 cut(s) 282, 298
BstPI GGTNACC 1 cut(s) 758
BstSCI CCNGG 3 cut(s) 280, 296, 548
BstSLI GKGCMC 2 cut(s) 119, 936
BstV1I GCAGC 1 cut(s) 703
BstX2I RGATCY 2 cut(s) 599, 638
BstXI CCANNNNNNTGG 1 cut(s) 127
BstYI RGATCY 2 cut(s) 599, 638
BsuRI GGCC 6 cut(s) 117, 199, 539, 573, 669, 723
BtgI CCRYGG 1 cut(s) 901
BtrI CACGTC 2 cut(s) 355, 415
BtsCI GGATG 2 cut(s) 181, 445
BtsI GCAGTG 1 cut(s) 51
BtsIMutI CAGTG 1 cut(s) 51
BveI ACCTGC 1 cut(s) 721
Cac8I GCNNGC 6 cut(s) 197, 224, 317, 537, 667, 721
CaiI CAGNNNCTG 1 cut(s) 956
Cfr10I RCCGGY 1 cut(s) 167
Cfr13I GGNCC 7 cut(s) 115, 116, 165, 212, 571, 668, 722
Csp6I GTAC 4 cut(s) 171, 208, 510, 555
CspAI ACCGGT 1 cut(s) 167
CviAII CATG 3 cut(s) 350, 457, 649
CviQI GTAC 4 cut(s) 171, 208, 510, 555
DdeI CTNAG 6 cut(s) 367, 388, 530, 615, 949, 957
DpnI GATC 5 cut(s) 332, 455, 601, 640, 713
DpnII GATC 5 cut(s) 330, 453, 599, 638, 711
Eam1104I CTCTTC 2 cut(s) 182, 296
EarI CTCTTC 2 cut(s) 182, 296
EciI GGCGGA 2 cut(s) 283, 882
Ecl136II GAGCTC 1 cut(s) 479
Eco130I CCWWGG 1 cut(s) 200
Eco147I AGGCCT 1 cut(s) 199
Eco24I GRGCYC 2 cut(s) 119, 481
Eco31I GGTCTC 1 cut(s) 900
Eco47I GGWCC 2 cut(s) 165, 212
Eco53kI GAGCTC 1 cut(s) 479
Eco57I CTGAAG 1 cut(s) 999
Eco88I CYCGRG 1 cut(s) 480
Eco91I GGTNACC 1 cut(s) 758
EcoICRI GAGCTC 1 cut(s) 479
EcoNI CCTNNNNNAGG 1 cut(s) 301
EcoO109I RGGNCCY 2 cut(s) 115, 116
EcoO65I GGTNACC 1 cut(s) 758
EcoRII CCWGG 2 cut(s) 280, 296
EcoT14I CCWWGG 1 cut(s) 200
EcoT22I ATGCAT 1 cut(s) 494
EcoT38I GRGCYC 2 cut(s) 119, 481
ErhI CCWWGG 1 cut(s) 200
FaeI CATG 3 cut(s) 353, 460, 652
FaqI GGGAC 3 cut(s) 178, 232, 803
FatI CATG 3 cut(s) 349, 456, 648
FauNDI CATATG 1 cut(s) 565
Fnu4HI GCNGC 2 cut(s) 574, 717
FokI GGATG 2 cut(s) 188, 452
FriOI GRGCYC 2 cut(s) 119, 481
Fsp4HI GCNGC 2 cut(s) 574, 717
FspBI CTAG 2 cut(s) 102, 201
GluI GCNGC 2 cut(s) 574, 717
GsuI CTGGAG 2 cut(s) 48, 303
HaeIII GGCC 6 cut(s) 117, 199, 539, 573, 669, 723
HapII CCGG 4 cut(s) 168, 232, 549, 642
Hin1I GRCGYC 1 cut(s) 143
Hin1II CATG 3 cut(s) 353, 460, 652
HincII GTYRAC 1 cut(s) 325
HindII GTYRAC 1 cut(s) 325
HinfI GANTC 5 cut(s) 271, 326, 792, 925, 986
HpaII CCGG 4 cut(s) 168, 232, 549, 642
HphI GGTGA 4 cut(s) 65, 383, 752, 857
Hpy166II GTNNAC 4 cut(s) 56, 325, 695, 934
Hpy188I TCNGA 5 cut(s) 138, 885, 924, 958, 979
Hpy188III TCNNGA 4 cut(s) 65, 334, 597, 943
Hpy8I GTNNAC 4 cut(s) 56, 325, 695, 934
Hpy99I CGWCG 4 cut(s) 148, 241, 268, 271
HpyAV CCTTC 3 cut(s) 850, 903, 907
HpyCH4III ACNGT 3 cut(s) 127, 677, 1013
HpyCH4IV ACGT 3 cut(s) 143, 354, 414
HpyCH4V TGCA 8 cut(s) 4, 383, 492, 503, 623, 730, 754, 934
HpyF10VI GCNNNNNNNGC 1 cut(s) 784
HpyF3I CTNAG 6 cut(s) 367, 388, 530, 615, 949, 957
HpySE526I ACGT 3 cut(s) 143, 354, 414
Hsp92I GRCGYC 1 cut(s) 143
Hsp92II CATG 3 cut(s) 353, 460, 652
KpnI GGTACC 1 cut(s) 513
Kzo9I GATC 5 cut(s) 330, 453, 599, 638, 711
LmnI GCTCC 5 cut(s) 67, 139, 282, 476, 895
Lsp1109I GCAGC 1 cut(s) 703
LweI GCATC 1 cut(s) 243
MaeI CTAG 2 cut(s) 102, 201
MaeII ACGT 3 cut(s) 143, 354, 414
MaeIII GTNAC 4 cut(s) 371, 442, 523, 758
MalI GATC 5 cut(s) 332, 455, 601, 640, 713
MbiI CCGCTC 1 cut(s) 51
MboI GATC 5 cut(s) 330, 453, 599, 638, 711
MboII GAAGA 8 cut(s) 193, 196, 199, 313, 463, 537, 860, 992
MfeI CAATTG 1 cut(s) 813
MflI RGATCY 2 cut(s) 599, 638
MhlI GDGCHC 3 cut(s) 119, 481, 936
MluCI AATT 5 cut(s) 70, 78, 378, 813, 916
MlyI GAGTC 2 cut(s) 320, 801
MnlI CCTC 8 cut(s) 4, 183, 297, 305, 420, 428, 430, 562
Mph1103I ATGCAT 1 cut(s) 494
MseI TTAA 1 cut(s) 560
MslI CAYNNNNRTG 2 cut(s) 125, 348
MspI CCGG 4 cut(s) 168, 232, 549, 642
MspR9I CCNGG 3 cut(s) 282, 298, 550
MunI CAATTG 1 cut(s) 813
Mva1269I GAATGC 1 cut(s) 625
MvaI CCWGG 2 cut(s) 282, 298
MwoI GCNNNNNNNGC 1 cut(s) 784
NciI CCSGG 1 cut(s) 550
NdeI CATATG 1 cut(s) 565
NdeII GATC 5 cut(s) 330, 453, 599, 638, 711
NlaIII CATG 3 cut(s) 353, 460, 652
NlaIV GGNNCC 8 cut(s) 116, 117, 118, 166, 214, 511, 724, 891
NmuCI GTSAC 2 cut(s) 371, 758
NsiI ATGCAT 1 cut(s) 494
OliI CACNNNNGTG 1 cut(s) 125
PaeR7I CTCGAG 1 cut(s) 480
PceI AGGCCT 1 cut(s) 199
PctI GAATGC 1 cut(s) 625
PfeI GAWTC 3 cut(s) 271, 925, 986
PinAI ACCGGT 1 cut(s) 167
PkrI GCNGC 2 cut(s) 575, 718
PleI GAGTC 2 cut(s) 320, 800
PpsI GAGTC 2 cut(s) 320, 800
Psp124BI GAGCTC 1 cut(s) 481
Psp6I CCWGG 2 cut(s) 280, 296
PspEI GGTNACC 1 cut(s) 758
PspGI CCWGG 2 cut(s) 280, 296
PspN4I GGNNCC 8 cut(s) 116, 117, 118, 166, 214, 511, 724, 891
PspOMI GGGCCC 1 cut(s) 115
PspPI GGNCC 7 cut(s) 115, 116, 165, 212, 571, 668, 722
PspXI VCTCGAGB 1 cut(s) 480
PstNI CAGNNNCTG 1 cut(s) 956
PsuI RGATCY 2 cut(s) 599, 638
RsaI GTAC 4 cut(s) 172, 209, 511, 556
RsaNI GTAC 4 cut(s) 171, 208, 510, 555
RseI CAYNNNNRTG 2 cut(s) 125, 348
SacI GAGCTC 1 cut(s) 481
SaqAI TTAA 1 cut(s) 560
SatI GCNGC 2 cut(s) 574, 717
Sau3AI GATC 5 cut(s) 330, 453, 599, 638, 711
Sau96I GGNCC 7 cut(s) 115, 116, 165, 212, 571, 668, 722
SchI GAGTC 2 cut(s) 320, 801
ScrFI CCNGG 3 cut(s) 282, 298, 550
SduI GDGCHC 3 cut(s) 119, 481, 936
SfaNI GCATC 1 cut(s) 243
Sfr274I CTCGAG 1 cut(s) 480
SinI GGWCC 2 cut(s) 165, 212
SlaI CTCGAG 1 cut(s) 480
SmiMI CAYNNNNRTG 2 cut(s) 125, 348
SmlI CTYRAG 1 cut(s) 480
SmoI CTYRAG 1 cut(s) 480
Sse9I AATT 5 cut(s) 70, 78, 378, 813, 916
SseBI AGGCCT 1 cut(s) 199
SsiI CCGC 4 cut(s) 51, 294, 574, 867
SspMI CTAG 2 cut(s) 102, 201
SstI GAGCTC 1 cut(s) 481
StuI AGGCCT 1 cut(s) 199
StyD4I CCNGG 3 cut(s) 280, 296, 548
StyI CCWWGG 1 cut(s) 200
TaaI ACNGT 3 cut(s) 127, 677, 1013
TaiI ACGT 3 cut(s) 146, 357, 417
TaqI TCGA 4 cut(s) 269, 329, 481, 795
TasI AATT 5 cut(s) 70, 78, 378, 813, 916
TauI GCSGC 1 cut(s) 576
TfiI GAWTC 3 cut(s) 271, 925, 986
Tru1I TTAA 1 cut(s) 560
Tru9I TTAA 1 cut(s) 560
TscAI CASTG 1 cut(s) 51
TseFI GTSAC 2 cut(s) 371, 758
TseI GCWGC 1 cut(s) 716
Tsp45I GTSAC 2 cut(s) 371, 758
TspDTI ATGAA 3 cut(s) 416, 464, 869
TspGWI ACGGA 3 cut(s) 188, 225, 918
TspRI CASTG 1 cut(s) 51
VneI GTGCAC 1 cut(s) 932
VpaK11BI GGWCC 2 cut(s) 165, 212
XagI CCTNNNNNAGG 1 cut(s) 301
XapI RAATTY 1 cut(s) 916
XhoI CTCGAG 1 cut(s) 480
XmaJI CCTAGG 1 cut(s) 200
XspI CTAG 2 cut(s) 102, 201
ZraI GACGTC 1 cut(s) 144
Zsp2I ATGCAT 1 cut(s) 494
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.