AT5G62280

Protein of unknown function (DUF1442)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0015385
Physical Location & Seq
Forward (+)
25016860 .. 25018265
1406 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G62280.1

Sequence Viewer

Length: 711 bp
ATGGCTTTTTGGTCTGCTGAGAATGCTACTAAAGCCTATCTTAGTACATTGAAAACGGATCAAAGGACAAAAGAACCAAACGTCGCCGAATTCATATCAGCCCTAGCCGCCGGAAATAGCGCTAGAAAGATAGCCGTGGCTTGCGCCGGAGCAGCAAACGCCGACATACTCGTTGCACTAATCGCAGCGGCTAACCAAACGCGTGGTCAAGTTGTATGCGTTTTACGTGGTATCGAAGAACTAATCATATCCCAAAAAATGTTGGAACCATCAGAGATTCATCAGATACAATTCGTAGTCGGAGAATCTAACGACGACACTCTAATCAATAATCATTTCGGAGAAGCAGATTTCGTCCTCGTGGATTGTAACCTCGAAAACCACCAAGAGATCGTTGGAAAAATCCTTAATCATCACGAAGAAAACGCAAGAACCGGGGGTGGAAGCGGTGTGGCGGTTGTGGTGGGTTATAACGCGTTTTCGAGAGGGTCATGGAGGTTTAGCGATGGAAGGAAAACGCAGTTTTTACCTATAGGAGAAGGGTTACTTGTGACGAGGGTCAATGATAATCAGAAGATGATGATGAAAAATCACCATCGTGACCAAGTGATGAGGAAGAGTCGTTGGGTGGTGAAGGTTGATAAGTGCACTGGAGAGGAACATGTGTTTAGGGTTAGGGTTCCCCGAGGAGAAGCCATTATTGAAGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1442

Protein Analysis

236

Amino Acids

26.01

Weight (kDa)

6.71

Isoelectric Point (pI)

24.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1442 PF07279 3 - 224 6.5e-24 Protein of unknown function (DUF1442)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015385)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G62280
fragaria_vesca FvH4_7g28130
malus_domestica MD01G1185000.v1.1 MD07G1257300.v1.1
prunus_persica Prupe.2G281500_v2.0.a1
pyrus_communis pycom01g19670
rosa_chinensis RchiOBHm_Chr1g0375051
rosa_laevigata RLG00000026692 RLG00000026693
rosa_roxburghii Rroxscaffold_4G00283060
rosa_rugosa Rorug01G0387800
rosa_samantha Rh1AG398400 Rh1BG362300 Rh1CG376000 Rh1DG393300
rosa_wichuraiana Rw1G035370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 471
AccII CGCG 2 cut(s) 202, 476
AciI CCGC 4 cut(s) 108, 188, 447, 455
AclWI GGATC 1 cut(s) 66
AcsI RAATTY 1 cut(s) 89
AfaI GTAC 1 cut(s) 46
AfeI AGCGCT 1 cut(s) 121
AflIII ACRYGT 3 cut(s) 200, 474, 661
AgsI TTSAA 2 cut(s) 52, 704
AleI CACNNNNGTG 1 cut(s) 597
AluBI AGCT 1 cut(s) 707
AluI AGCT 1 cut(s) 707
Alw21I GWGCWC 1 cut(s) 650
Alw44I GTGCAC 1 cut(s) 646
AlwI GGATC 1 cut(s) 66
Ama87I CYCGRG 1 cut(s) 684
Aor51HI AGCGCT 1 cut(s) 121
ApaLI GTGCAC 1 cut(s) 646
ApeKI GCWGC 2 cut(s) 152, 185
ApoI RAATTY 1 cut(s) 89
ArsI GACNNNNNNTTYG 2 cut(s) 190, 222
AspLEI GCGC 2 cut(s) 122, 146
AsuC2I CCSGG 1 cut(s) 436
AsuHPI GGTGA 2 cut(s) 584, 643
AvaI CYCGRG 1 cut(s) 684
BaeGI GKGCMC 1 cut(s) 650
BauI CACGAG 1 cut(s) 359
Bbv12I GWGCWC 1 cut(s) 650
BbvI GCAGC 2 cut(s) 164, 197
BccI CCATC 3 cut(s) 277, 500, 603
BceAI ACGGC 1 cut(s) 119
BcnI CCSGG 1 cut(s) 436
BfaI CTAG 2 cut(s) 104, 123
BfmI CTRYAG 1 cut(s) 531
BfoI RGCGCY 1 cut(s) 123
BisI GCNGC 4 cut(s) 108, 153, 186, 189
BlsI GCNGC 4 cut(s) 109, 154, 187, 190
Bme1390I CCNGG 1 cut(s) 436
BmeT110I CYCGRG 1 cut(s) 684
BmiI GGNNCC 2 cut(s) 267, 681
BmrFI CCNGG 1 cut(s) 436
BoxI GACNNNNGTC 1 cut(s) 557
BpmI CTGGAG 1 cut(s) 672
BpuMI CCSGG 1 cut(s) 436
BsaAI YACGTR 1 cut(s) 227
BsaJI CCNNGG 3 cut(s) 135, 435, 685
Bse1I ACTGG 1 cut(s) 655
BseDI CCNNGG 3 cut(s) 135, 435, 685
BseMII CTCAG 1 cut(s) 9
BseNI ACTGG 1 cut(s) 655
BseRI GAGGAG 1 cut(s) 702
BseSI GKGCMC 1 cut(s) 650
BseXI GCAGC 2 cut(s) 164, 197
Bsh1236I CGCG 2 cut(s) 202, 476
BsiHKAI GWGCWC 1 cut(s) 650
BsiHKCI CYCGRG 1 cut(s) 684
BsiSI CCGG 3 cut(s) 111, 147, 435
BsmI GAATGC 1 cut(s) 28
BsoBI CYCGRG 1 cut(s) 684
Bsp1286I GDGCHC 1 cut(s) 650
Bsp143I GATC 2 cut(s) 58, 390
BspACI CCGC 4 cut(s) 108, 188, 447, 455
BspCNI CTCAG 1 cut(s) 10
BspFNI CGCG 2 cut(s) 202, 476
BspLI GGNNCC 2 cut(s) 267, 681
BspPI GGATC 1 cut(s) 66
BsrI ACTGG 1 cut(s) 655
BssECI CCNNGG 3 cut(s) 135, 435, 685
BssMI GATC 2 cut(s) 58, 390
BssSI CACGAG 1 cut(s) 359
Bst2BI CACGAG 1 cut(s) 359
Bst6I CTCTTC 1 cut(s) 611
BstBAI YACGTR 1 cut(s) 227
BstC8I GCNNGC 1 cut(s) 142
BstDEI CTNAG 2 cut(s) 18, 41
BstDSI CCRYGG 1 cut(s) 135
BstFNI CGCG 2 cut(s) 202, 476
BstH2I RGCGCY 1 cut(s) 123
BstHHI GCGC 2 cut(s) 122, 146
BstKTI GATC 2 cut(s) 61, 393
BstMBI GATC 2 cut(s) 58, 390
BstMWI GCNNNNNNNGC 6 cut(s) 23, 32, 107, 152, 158, 182
BstNSI RCATGY 1 cut(s) 665
BstPAI GACNNNNGTC 1 cut(s) 557
BstSCI CCNGG 1 cut(s) 434
BstSFI CTRYAG 1 cut(s) 531
BstSLI GKGCMC 1 cut(s) 650
BstUI CGCG 2 cut(s) 202, 476
BstV1I GCAGC 2 cut(s) 164, 197
BstXI CCANNNNNNTGG 1 cut(s) 203
BtgI CCRYGG 1 cut(s) 135
BtgZI GCGATG 1 cut(s) 519
BtsIMutI CAGTG 1 cut(s) 648
Cac8I GCNNGC 1 cut(s) 142
CfoI GCGC 2 cut(s) 122, 146
Csp6I GTAC 1 cut(s) 45
CviAII CATG 2 cut(s) 492, 662
CviJI RGCY 9 cut(s) 5, 35, 101, 107, 134, 140, 191, 695, 707
CviKI_1 RGCY 9 cut(s) 5, 35, 101, 107, 134, 140, 191, 695, 707
CviQI GTAC 1 cut(s) 45
DdeI CTNAG 2 cut(s) 18, 41
DpnI GATC 2 cut(s) 60, 392
DpnII GATC 2 cut(s) 58, 390
Eam1104I CTCTTC 1 cut(s) 611
EarI CTCTTC 1 cut(s) 611
Eco47III AGCGCT 1 cut(s) 121
Eco88I CYCGRG 1 cut(s) 684
EcoRI GAATTC 1 cut(s) 89
FaeI CATG 2 cut(s) 495, 665
FaiI YATR 8 cut(s) 95, 167, 217, 248, 471, 493, 533, 663
FalI AAGNNNNNCTT 4 cut(s) 24, 56, 531, 563
FatI CATG 2 cut(s) 491, 661
Fnu4HI GCNGC 4 cut(s) 108, 153, 186, 189
Fsp4HI GCNGC 4 cut(s) 108, 153, 186, 189
FspBI CTAG 2 cut(s) 104, 123
GlaI GCGC 2 cut(s) 121, 145
GluI GCNGC 4 cut(s) 108, 153, 186, 189
GsuI CTGGAG 1 cut(s) 672
HaeII RGCGCY 1 cut(s) 123
HapII CCGG 3 cut(s) 111, 147, 435
HhaI GCGC 2 cut(s) 122, 146
Hin1II CATG 2 cut(s) 495, 665
Hin6I GCGC 2 cut(s) 120, 144
HinP1I GCGC 2 cut(s) 120, 144
HindIII AAGCTT 1 cut(s) 705
HinfI GANTC 3 cut(s) 277, 305, 619
HpaII CCGG 3 cut(s) 111, 147, 435
HphI GGTGA 2 cut(s) 584, 643
Hpy166II GTNNAC 1 cut(s) 648
Hpy188I TCNGA 5 cut(s) 274, 285, 302, 341, 573
Hpy188III TCNNGA 3 cut(s) 416, 483, 599
Hpy8I GTNNAC 1 cut(s) 648
Hpy99I CGWCG 2 cut(s) 86, 317
HpyAV CCTTC 3 cut(s) 504, 533, 628
HpyCH4IV ACGT 2 cut(s) 81, 226
HpyCH4V TGCA 2 cut(s) 176, 648
HpyF10VI GCNNNNNNNGC 6 cut(s) 23, 32, 107, 152, 158, 182
HpyF3I CTNAG 2 cut(s) 18, 41
HpySE526I ACGT 2 cut(s) 81, 226
Hsp92II CATG 2 cut(s) 495, 665
HspAI GCGC 2 cut(s) 120, 144
Kzo9I GATC 2 cut(s) 58, 390
LmnI GCTCC 1 cut(s) 149
LpnPI CCDG 4 cut(s) 124, 160, 448, 636
Lsp1109I GCAGC 2 cut(s) 164, 197
MaeI CTAG 2 cut(s) 104, 123
MaeII ACGT 2 cut(s) 81, 226
MaeIII GTNAC 4 cut(s) 368, 543, 550, 599
MalI GATC 2 cut(s) 60, 392
MboI GATC 2 cut(s) 58, 390
MboII GAAGA 4 cut(s) 248, 431, 586, 628
MhlI GDGCHC 1 cut(s) 650
MluCI AATT 2 cut(s) 89, 290
MluI ACGCGT 2 cut(s) 200, 474
MlyI GAGTC 1 cut(s) 628
MmeI TCCRAC 3 cut(s) 243, 280, 376
MnlI CCTC 8 cut(s) 368, 383, 479, 489, 549, 606, 649, 680
MseI TTAA 2 cut(s) 408, 709
MslI CAYNNNNRTG 1 cut(s) 597
MspA1I CMGCKG 1 cut(s) 188
MspI CCGG 3 cut(s) 111, 147, 435
MspR9I CCNGG 1 cut(s) 436
Mva1269I GAATGC 1 cut(s) 28
MvnI CGCG 2 cut(s) 202, 476
MwoI GCNNNNNNNGC 6 cut(s) 23, 32, 107, 152, 158, 182
NciI CCSGG 1 cut(s) 436
NdeII GATC 2 cut(s) 58, 390
NlaIII CATG 2 cut(s) 495, 665
NlaIV GGNNCC 2 cut(s) 267, 681
NmuCI GTSAC 2 cut(s) 550, 599
NspI RCATGY 1 cut(s) 665
OliI CACNNNNGTG 1 cut(s) 597
PciI ACATGT 1 cut(s) 661
PctI GAATGC 1 cut(s) 28
PfeI GAWTC 2 cut(s) 277, 305
PkrI GCNGC 4 cut(s) 109, 154, 187, 190
PleI GAGTC 1 cut(s) 627
PpsI GAGTC 1 cut(s) 627
Ppu21I YACGTR 1 cut(s) 227
PscI ACATGT 1 cut(s) 661
PshAI GACNNNNGTC 1 cut(s) 557
PsiI TTATAA 1 cut(s) 471
PspN4I GGNNCC 2 cut(s) 267, 681
RsaI GTAC 1 cut(s) 46
RsaNI GTAC 1 cut(s) 45
RseI CAYNNNNRTG 1 cut(s) 597
SaqAI TTAA 2 cut(s) 408, 709
SatI GCNGC 4 cut(s) 108, 153, 186, 189
Sau3AI GATC 2 cut(s) 58, 390
SchI GAGTC 1 cut(s) 628
ScrFI CCNGG 1 cut(s) 436
SduI GDGCHC 1 cut(s) 650
SetI ASST 7 cut(s) 84, 229, 375, 500, 532, 639, 709
SfcI CTRYAG 1 cut(s) 531
SmiMI CAYNNNNRTG 1 cut(s) 597
Sse9I AATT 2 cut(s) 89, 290
SsiI CCGC 4 cut(s) 108, 188, 447, 455
SspMI CTAG 2 cut(s) 104, 123
StyD4I CCNGG 1 cut(s) 434
TaiI ACGT 2 cut(s) 84, 229
TaqI TCGA 3 cut(s) 234, 375, 482
TasI AATT 2 cut(s) 89, 290
TatI WGTACW 1 cut(s) 44
TauI GCSGC 2 cut(s) 110, 191
TfiI GAWTC 2 cut(s) 277, 305
Tru1I TTAA 2 cut(s) 408, 709
Tru9I TTAA 2 cut(s) 408, 709
TscAI CASTG 1 cut(s) 655
TseFI GTSAC 2 cut(s) 550, 599
TseI GCWGC 2 cut(s) 152, 185
Tsp45I GTSAC 2 cut(s) 550, 599
TspDTI ATGAA 3 cut(s) 82, 269, 599
TspGWI ACGGA 1 cut(s) 71
TspRI CASTG 1 cut(s) 655
VneI GTGCAC 1 cut(s) 646
XapI RAATTY 1 cut(s) 89
XceI RCATGY 1 cut(s) 665
XcmI CCANNNNNNNNNTGG 1 cut(s) 392
XspI CTAG 2 cut(s) 104, 123
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.