MD07G1257300.v1.1

Protein of unknown function (DUF1442)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0015385
Physical Location & Seq
Reverse (-)
32363235 .. 32364097
863 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1257300.v1.1.491

Sequence Viewer

Length: 657 bp
ATGGCTTGCTGGTCTGCTGAGAATGCCACAAAAGCCTACCTCAAAGCCCTAAAAATGGGCCAGAAGGGAAACGAGCCAGATGTGATCGAGTTCATTTCAGCCCTAGCAGCAGGCAACAATGCGCAGCTTATGGTTGTTGCATGCGCTGGAGCTGCCGACTCCAAAACCCTAGCCCTAGCTGCCGCCGCTCAACAAACTGGCAGCAGAGTTGTATGCATTCTTCGCGGTAACGAAGAATTACACTTGTCTGAGAAAATCCTCGGCGTCAACGTTTGTCACGTTGAGTTTGTTATTGGAGAAGCTCAAAATCTTCTTTTAAATTACTACAAGGAGGCGGATTTCGTGCTCATCGATTGCAACCTCAAGAACCACGAGGCAGTTCTTAGAGCGGTGCAGATGGGGAGGAAGCAAAACGGAGCTATTGTTGTGGGGCATAACGCTTTCGGAAGAGGGTCGTGGAGATCCGGCGGATCAAGGACTCAGTTGTTGCCCATCGGAGGTGGGTTGCTGGTGACAAGAATTGCAGCAGAAAGTAATAATGCAAAAACGACTGGAAAAAAGAGTCATTGGGTTGTGAAGGTCGATAAATGCACAGGTGAAGAGCACGTGTTTAGGGTTAGATCATCGTTTCCATATGGGAAAGGGATTGCAACTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1442

Protein Analysis

219

Amino Acids

23.31

Weight (kDa)

9.17

Isoelectric Point (pI)

31.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1442 PF07279 3 - 205 1.6e-29 Protein of unknown function (DUF1442)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015385)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G62280
fragaria_vesca FvH4_7g28130
malus_domestica MD01G1185000.v1.1 MD07G1257300.v1.1
prunus_persica Prupe.2G281500_v2.0.a1
pyrus_communis pycom01g19670
rosa_chinensis RchiOBHm_Chr1g0375051
rosa_laevigata RLG00000026692 RLG00000026693
rosa_roxburghii Rroxscaffold_4G00283060
rosa_rugosa Rorug01G0387800
rosa_samantha Rh1AG398400 Rh1BG362300 Rh1CG376000 Rh1DG393300
rosa_wichuraiana Rw1G035370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 123
AccBSI CCGCTC 2 cut(s) 188, 389
AccII CGCG 1 cut(s) 225
AciI CCGC 6 cut(s) 183, 186, 225, 335, 389, 468
AclI AACGTT 1 cut(s) 270
AclWI GGATC 2 cut(s) 456, 478
AcvI CACGTG 1 cut(s) 607
AcyI GRCGYC 1 cut(s) 264
AfiI CCNNNNNNNGG 2 cut(s) 55, 497
AflIII ACRYGT 1 cut(s) 606
AluBI AGCT 5 cut(s) 127, 152, 179, 302, 419
AluI AGCT 5 cut(s) 127, 152, 179, 302, 419
Alw21I GWGCWC 2 cut(s) 348, 606
AlwI GGATC 2 cut(s) 456, 478
AoxI GGCC 1 cut(s) 58
ApeKI GCWGC 6 cut(s) 107, 124, 152, 179, 201, 524
AspLEI GCGC 2 cut(s) 124, 146
AspS9I GGNCC 1 cut(s) 58
AsuHPI GGTGA 2 cut(s) 523, 608
BarI GAAGNNNNNNTAC 2 cut(s) 204, 236
BauI CACGAG 1 cut(s) 371
BbrPI CACGTG 1 cut(s) 607
Bbv12I GWGCWC 2 cut(s) 348, 606
BbvI GCAGC 6 cut(s) 119, 136, 139, 166, 213, 536
BccI CCATC 2 cut(s) 391, 500
BfaI CTAG 3 cut(s) 104, 170, 176
BisI GCNGC 8 cut(s) 108, 125, 153, 180, 183, 186, 202, 525
BlsI GCNGC 8 cut(s) 109, 126, 154, 181, 184, 187, 203, 526
BmgT120I GGNCC 1 cut(s) 58
BpmI CTGGAG 1 cut(s) 168
BpuEI CTTGAG 1 cut(s) 347
Bsa29I ATCGAT 1 cut(s) 351
BsaAI YACGTR 1 cut(s) 607
BsaHI GRCGYC 1 cut(s) 264
BsaJI CCNNGG 1 cut(s) 259
BsaXI ACNNNNNCTCC 2 cut(s) 408, 438
Bsc4I CCNNNNNNNGG 2 cut(s) 55, 497
Bse1I ACTGG 2 cut(s) 202, 556
BseCI ATCGAT 1 cut(s) 351
BseDI CCNNGG 1 cut(s) 259
BseLI CCNNNNNNNGG 2 cut(s) 55, 497
BseMII CTCAG 3 cut(s) 9, 240, 494
BseNI ACTGG 2 cut(s) 202, 556
BseXI GCAGC 6 cut(s) 119, 136, 139, 166, 213, 536
BsgI GTGCAG 1 cut(s) 413
Bsh1236I CGCG 1 cut(s) 225
BshFI GGCC 1 cut(s) 60
BshVI ATCGAT 1 cut(s) 351
BsiHKAI GWGCWC 2 cut(s) 348, 606
BsiSI CCGG 1 cut(s) 465
BslI CCNNNNNNNGG 2 cut(s) 55, 497
BsmI GAATGC 2 cut(s) 28, 216
BsnI GGCC 1 cut(s) 60
Bsp1286I GDGCHC 2 cut(s) 348, 606
Bsp143I GATC 4 cut(s) 84, 461, 470, 620
BspACI CCGC 6 cut(s) 183, 186, 225, 335, 389, 468
BspANI GGCC 1 cut(s) 60
BspCNI CTCAG 3 cut(s) 10, 241, 493
BspDI ATCGAT 1 cut(s) 351
BspFNI CGCG 1 cut(s) 225
BspPI GGATC 2 cut(s) 456, 478
BspQI GCTCTTC 1 cut(s) 594
BsrBI CCGCTC 2 cut(s) 188, 389
BsrI ACTGG 2 cut(s) 202, 556
BssECI CCNNGG 1 cut(s) 259
BssMI GATC 4 cut(s) 84, 461, 470, 620
BssNI GRCGYC 1 cut(s) 264
BssSI CACGAG 1 cut(s) 371
Bst2BI CACGAG 1 cut(s) 371
Bst6I CTCTTC 2 cut(s) 442, 594
BstACI GRCGYC 1 cut(s) 264
BstBAI YACGTR 1 cut(s) 607
BstC8I GCNNGC 3 cut(s) 7, 112, 142
BstDEI CTNAG 5 cut(s) 18, 249, 383, 480, 654
BstFNI CGCG 1 cut(s) 225
BstHHI GCGC 2 cut(s) 124, 146
BstKTI GATC 4 cut(s) 87, 464, 473, 623
BstMBI GATC 4 cut(s) 84, 461, 470, 620
BstMWI GCNNNNNNNGC 7 cut(s) 23, 32, 107, 152, 179, 185, 222
BstNSI RCATGY 1 cut(s) 144
BstUI CGCG 1 cut(s) 225
BstV1I GCAGC 6 cut(s) 119, 136, 139, 166, 213, 536
BstX2I RGATCY 1 cut(s) 461
BstYI RGATCY 1 cut(s) 461
Bsu15I ATCGAT 1 cut(s) 351
BsuRI GGCC 1 cut(s) 60
BsuTUI ATCGAT 1 cut(s) 351
Cac8I GCNNGC 3 cut(s) 7, 112, 142
CfoI GCGC 2 cut(s) 124, 146
Cfr13I GGNCC 1 cut(s) 58
ClaI ATCGAT 1 cut(s) 351
CseI GACGC 1 cut(s) 253
CviAII CATG 1 cut(s) 141
DdeI CTNAG 5 cut(s) 18, 249, 383, 480, 654
DpnI GATC 4 cut(s) 86, 463, 472, 622
DpnII GATC 4 cut(s) 84, 461, 470, 620
DraI TTTAAA 1 cut(s) 318
Eam1104I CTCTTC 2 cut(s) 442, 594
EarI CTCTTC 2 cut(s) 442, 594
EciI GGCGGA 2 cut(s) 350, 483
Eco72I CACGTG 1 cut(s) 607
EcoT22I ATGCAT 1 cut(s) 218
FaeI CATG 1 cut(s) 144
FaiI YATR 6 cut(s) 131, 142, 214, 435, 634, 636
FatI CATG 1 cut(s) 140
FauNDI CATATG 1 cut(s) 634
Fnu4HI GCNGC 8 cut(s) 108, 125, 153, 180, 183, 186, 202, 525
Fsp4HI GCNGC 8 cut(s) 108, 125, 153, 180, 183, 186, 202, 525
FspBI CTAG 3 cut(s) 104, 170, 176
FspI TGCGCA 1 cut(s) 123
GlaI GCGC 2 cut(s) 123, 145
GluI GCNGC 8 cut(s) 108, 125, 153, 180, 183, 186, 202, 525
GsuI CTGGAG 1 cut(s) 168
HaeIII GGCC 1 cut(s) 60
HapII CCGG 1 cut(s) 465
HgaI GACGC 1 cut(s) 253
HhaI GCGC 2 cut(s) 124, 146
Hin1I GRCGYC 1 cut(s) 264
Hin1II CATG 1 cut(s) 144
Hin6I GCGC 2 cut(s) 122, 144
HinP1I GCGC 2 cut(s) 122, 144
HincII GTYRAC 1 cut(s) 268
HindII GTYRAC 1 cut(s) 268
HinfI GANTC 3 cut(s) 158, 478, 562
HpaII CCGG 1 cut(s) 465
HphI GGTGA 2 cut(s) 523, 608
Hpy166II GTNNAC 1 cut(s) 268
Hpy188I TCNGA 3 cut(s) 250, 446, 497
Hpy188III TCNNGA 1 cut(s) 364
Hpy8I GTNNAC 1 cut(s) 268
HpyAV CCTTC 2 cut(s) 58, 571
HpyCH4IV ACGT 3 cut(s) 270, 279, 606
HpyCH4V TGCA 8 cut(s) 140, 216, 357, 394, 524, 542, 591, 650
HpyF10VI GCNNNNNNNGC 7 cut(s) 23, 32, 107, 152, 179, 185, 222
HpyF3I CTNAG 5 cut(s) 18, 249, 383, 480, 654
HpySE526I ACGT 3 cut(s) 270, 279, 606
Hsp92I GRCGYC 1 cut(s) 264
Hsp92II CATG 1 cut(s) 144
HspAI GCGC 2 cut(s) 122, 144
Kzo9I GATC 4 cut(s) 84, 461, 470, 620
LguI GCTCTTC 1 cut(s) 594
LmnI GCTCC 2 cut(s) 149, 416
LpnPI CCDG 9 cut(s) 74, 90, 96, 132, 183, 478, 494, 537, 579
Lsp1109I GCAGC 6 cut(s) 119, 136, 139, 166, 213, 536
MaeI CTAG 3 cut(s) 104, 170, 176
MaeII ACGT 3 cut(s) 270, 279, 606
MaeIII GTNAC 3 cut(s) 227, 275, 511
MalI GATC 4 cut(s) 86, 463, 472, 622
MbiI CCGCTC 2 cut(s) 188, 389
MboI GATC 4 cut(s) 84, 461, 470, 620
MboII GAAGA 5 cut(s) 212, 245, 302, 459, 611
MflI RGATCY 1 cut(s) 461
MhlI GDGCHC 2 cut(s) 348, 606
MluCI AATT 3 cut(s) 236, 319, 519
MlyI GAGTC 3 cut(s) 152, 472, 571
MnlI CCTC 8 cut(s) 50, 269, 325, 367, 371, 396, 443, 491
Mph1103I ATGCAT 1 cut(s) 218
MseI TTAA 1 cut(s) 317
MspI CCGG 1 cut(s) 465
Mva1269I GAATGC 2 cut(s) 28, 216
MvnI CGCG 1 cut(s) 225
MwoI GCNNNNNNNGC 7 cut(s) 23, 32, 107, 152, 179, 185, 222
NdeI CATATG 1 cut(s) 634
NdeII GATC 4 cut(s) 84, 461, 470, 620
NlaIII CATG 1 cut(s) 144
NmeAIII GCCGAG 1 cut(s) 240
NmuCI GTSAC 2 cut(s) 275, 511
NsbI TGCGCA 1 cut(s) 123
NsiI ATGCAT 1 cut(s) 218
NspI RCATGY 1 cut(s) 144
PaeI GCATGC 1 cut(s) 144
PciSI GCTCTTC 1 cut(s) 594
PcsI WCGNNNNNNNCGW 3 cut(s) 267, 276, 348
PctI GAATGC 2 cut(s) 28, 216
PkrI GCNGC 8 cut(s) 109, 126, 154, 181, 184, 187, 203, 526
PleI GAGTC 3 cut(s) 152, 472, 570
PmaCI CACGTG 1 cut(s) 607
PmlI CACGTG 1 cut(s) 607
PpsI GAGTC 3 cut(s) 152, 472, 570
Ppu21I YACGTR 1 cut(s) 607
Psp1406I AACGTT 1 cut(s) 270
PspCI CACGTG 1 cut(s) 607
PspPI GGNCC 1 cut(s) 58
PsuI RGATCY 1 cut(s) 461
SapI GCTCTTC 1 cut(s) 594
SaqAI TTAA 1 cut(s) 317
SatI GCNGC 8 cut(s) 108, 125, 153, 180, 183, 186, 202, 525
Sau3AI GATC 4 cut(s) 84, 461, 470, 620
Sau96I GGNCC 1 cut(s) 58
SchI GAGTC 3 cut(s) 152, 472, 571
SduI GDGCHC 2 cut(s) 348, 606
SmlI CTYRAG 1 cut(s) 362
SmoI CTYRAG 1 cut(s) 362
SphI GCATGC 1 cut(s) 144
Sse9I AATT 3 cut(s) 236, 319, 519
SsiI CCGC 6 cut(s) 183, 186, 225, 335, 389, 468
SspMI CTAG 3 cut(s) 104, 170, 176
TaiI ACGT 3 cut(s) 273, 282, 609
TaqI TCGA 3 cut(s) 87, 351, 582
TasI AATT 3 cut(s) 236, 319, 519
TauI GCSGC 2 cut(s) 185, 188
Tru1I TTAA 1 cut(s) 317
Tru9I TTAA 1 cut(s) 317
TseFI GTSAC 2 cut(s) 275, 511
TseI GCWGC 6 cut(s) 107, 124, 152, 179, 201, 524
Tsp45I GTSAC 2 cut(s) 275, 511
TspDTI ATGAA 1 cut(s) 82
TspGWI ACGGA 1 cut(s) 429
XceI RCATGY 1 cut(s) 144
XspI CTAG 3 cut(s) 104, 170, 176
Zsp2I ATGCAT 1 cut(s) 218
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.