Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0005375

Physical Location & Seq

Genomic Coordinates Forward (+)

25765712 .. 25770618

Gene Structure 4907 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT5G64440.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1824 bp

ATGGGTAAGTATCAGGTCATGAAACGTGCAAGTGAGGTTGATCTTTCTACTGTCAAATATAAAGCTGAAACCATGAAAGCTCCTCATTTGACTGGCCTTTCCTTCAAGTTGTTCGTTAATTTGCTTGAAGCACCACTTATAGGCTCTTTGATTGTTGATTATTTGAAGAAAGACAATGGCATGACAAAGATTTTTCGCAACACAGTTATACCAGAAGAGCCCATGTTTAGACCGGAGTTCCCATCTCAAGAACCGGAGCATGATGTTGTCATTGTTGGCGAAGATGAAAGTCCTATAGACAGATTGGAAACAGCCTTGAAATGTCTTCCTCAGTATGATCCTTCTCGTAGCTTGCATGCAGATCCAGTGTCATCTTTCCGGTACTGGAAGATTCGTGATTATGCATATGCCTATAGATCTAAGCTGACAACTCCATTGCAGGTAGCAAAAAGAATAATCTCAATCATAGAGGAGTTTGGCTATGACAAGCCTCCAACACCATTTTTGATTAGATTTGATGCCAATGAAGTCATAAAGCAAGCTGAAGCTTCTACACGGAGGTTTGAACAAGGAAATCCAATATCTGTTTTGGATGGAATATTTGTGACAATCAAGGACGATATTGATTGTTTACCCCATCCGACAAATGGTGGAACAACATGGCTGCATGAGGATCGTTCTGTGGAGAAGGATTCAGCTGTTGTTTCAAAACTGCGTTCTTGTGGTGCAATCTTACTTGGCAAGGCAAATATGCATGAGTTAGGCATGGGGACCACCGGGAACAATTCAAATTACGGAACCACAAGAAACCCGCATGATCCTAAAAGGTACACGGGCGGATCTTCCTCAGGTTCAGCAGCTATTGTAGCCGCTGGACTATGTTCAGCTGCTCTAGGAACAGATGGTGGAGGTTCCGTTCGCATTCCTTCAGCACTTTGTGGTATAACGGGACTGAAGACAACATATGGTCGGACAGATATGACAGGGTCATTATGTGAAGGTGGAACAGTGGAAATAATTGGTCCACTTGCTTCATCTCTGGAAGATGCCTTCTTGGTGTATGCTGCAATCTTGGGTTCTTCATCTGCTGATAGATATAATTTGAAACCGAGCCCACCGTGTTTTCCAAAGTTATTGTCTCACAACGGAAGCAATGCAATAGGATCTCTACGACTAGGGAAATATACAAAGTGGTTTAATGATGTCAGTTCAAGTGACATCTCTGACAAATGCGAAGACATCCTTAAGCTCCTATCAAACAATCACGGTTGCAAAGTGGTGGAGATAGTGGTTCCTGAACTGGAAGAGATGCGTGCAGCCCATGTTATTTCGATTGGGTCTCCAACACTGTCTTCTCTTACTCCCTACTGTGAAGCTGGGAAAAATTCAAAACTAAGTTATGACACTCGTACCAGCTTTGCAATTTTCCGTTCATTCTCTGCTTCAGACTATATCGCTGCTCAATGTCTTAGGCGAAGATTGATGGAGTATCACTTGAATATCTTCAAAGACGTTGATGTCATTGTGACCCCTACAACTGGAATGACAGCTCCAGTGATACCTCCTGATGCTCTCAAAAATGGAGAAACCAATATTCAAGTGACAACTGATTTAATGCGCTTCGTTCTAGCTGCAAATCTCCTCGGCTTCCCTGCCATATCAGTCCCGGTTGGTTATGATAAAGAGGGGCTTCCTATAGGATTACAAATAATGGGAAGACCTTGGGCCGAAGCTACCGTCCTTGGTTTAGCTGCCGCAGTCGAGGAACTGGCTCCAGTTACCAAGAAACCTGCAATCTTTTATGATATTCTCAATACAAACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005783 GO:0005794 GO:0005886 GO:0006066 GO:0006576 GO:0006807 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0009308 GO:0009605 GO:0009607 GO:0009617 GO:0009987 GO:0012505 GO:0016020 GO:0016787 GO:0016810 GO:0016811 GO:0031090 GO:0034308 GO:0034641 GO:0042439 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044106 GO:0044237 GO:0044281 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0047412 GO:0050896 GO:0051704 GO:0051707 GO:0070291 GO:0071704 GO:0071944 GO:0098542 GO:0098588 GO:0098805 GO:1901564 GO:1901615

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Amidase

Protein Analysis

607

Amino Acids

66.08

Weight (kDa)

6.18

Isoelectric Point (pI)

38.31

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Amidase	PF01425	170 - 581	3.8e-87	Amidase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005375)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G64440
fragaria_vesca	FvH4_2g11120 FvH4_2g11120 FvH4_2g11120 FvH4_2g11120 FvH4_2g11120 FvH4_2g11120 FvH4_2g11120 FvH4_2g11120 FvH4_2g11120
malus_domestica	MD05G1050200.v1.1
prunus_persica	Prupe.8G076300_v2.0.a1 Prupe.8G076300_v2.0.a1 Prupe.8G076300_v2.0.a1 Prupe.8G076300_v2.0.a1
pyrus_communis	pycom05g04210
rosa_chinensis	RchiOBHm_Chr6g0269381
rosa_laevigata	RLG00000013854
rosa_multiflora	Rmu_sc0003735.1_g000029 Rmu_sc0015769.1_g000001 Rmu_ssc0000291.1_g000010
rosa_roxburghii	Rroxscaffold_7G00198770
rosa_rugosa	Rorug06G0047400
rosa_samantha	Rh6AG167800 Rh6AG331500 Rh6BG173100 Rh6CG167200 Rh6DG159000
rosa_wichuraiana	Rw6G028780

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1517
Acc36I	ACCTGC	2 cut(s)	430, 1798
AciI	CCGC	4 cut(s)	812, 837, 870, 1755
AclWI	GGATC	6 cut(s)	332, 356, 681, 812, 847, 1171
AcsI	RAATTY	1 cut(s)	1384
AcuI	CTGAAG	4 cut(s)	564, 912, 974, 1428
AdeI	CACNNNGTG	1 cut(s)	938
AfaI	GTAC	3 cut(s)	383, 830, 1411
AfiI	CCNNNNNNNGG	3 cut(s)	140, 647, 1538
AflII	CTTAAG	1 cut(s)	1244
AjuI	GAANNNNNNNTTGG	2 cut(s)	1336, 1368
AloI	GAACNNNNNNTCC	2 cut(s)	900, 932
Alw26I	GTCTC	2 cut(s)	1143, 1344
AlwI	GGATC	6 cut(s)	332, 356, 681, 812, 847, 1171
AoxI	GGCC	2 cut(s)	94, 1725
ApeKI	GCWGC	8 cut(s)	664, 857, 887, 1064, 1316, 1457, 1631, 1751
ApoI	RAATTY	1 cut(s)	1384
ArsI	GACNNNNNNTTYG	4 cut(s)	1121, 1153, 1722, 1754
Asp700I	GAANNNNTTC	1 cut(s)	1502
AspLEI	GCGC	1 cut(s)	1620
AspS9I	GGNCC	3 cut(s)	771, 1022, 1725
AsuC2I	CCSGG	2 cut(s)	778, 1667
AvaII	GGWCC	2 cut(s)	771, 1022
AxyI	CCTNAGG	1 cut(s)	847
BaeI	ACNNNNGTAYC	2 cut(s)	1393, 1426
BanII	GRGCYC	2 cut(s)	222, 1115
BbsI	GAAGAC	5 cut(s)	317, 962, 1242, 1344, 1723
BbvI	GCAGC	8 cut(s)	651, 869, 874, 1051, 1328, 1444, 1618, 1738
BccI	CCATC	5 cut(s)	250, 587, 645, 896, 1477
BcgI	CGANNNNNNTGC	2 cut(s)	656, 690
BcnI	CCSGG	2 cut(s)	778, 1667
BcoDI	GTCTC	2 cut(s)	1143, 1344
BfaI	CTAG	3 cut(s)	893, 1175, 1628
BfmI	CTRYAG	3 cut(s)	294, 412, 1695
BfrI	CTTAAG	1 cut(s)	1244
BfuAI	ACCTGC	2 cut(s)	430, 1798
BglII	AGATCT	1 cut(s)	416
Bme1390I	CCNGG	2 cut(s)	778, 1667
Bme18I	GGWCC	2 cut(s)	771, 1022
BmgT120I	GGNCC	3 cut(s)	771, 1022, 1725
BmiI	GGNNCC	5 cut(s)	772, 799, 913, 1293, 1773
BmrFI	CCNGG	2 cut(s)	778, 1667
BmsI	GCATC	4 cut(s)	508, 1036, 1299, 1558
BpiI	GAAGAC	5 cut(s)	317, 962, 1242, 1344, 1723
BpmI	CTGGAG	2 cut(s)	1536, 1758
BpuEI	CTTGAG	1 cut(s)	231
BpuMI	CCSGG	2 cut(s)	778, 1667
BsaI	GGTCTC	1 cut(s)	1344
BsaJI	CCNNGG	3 cut(s)	1642, 1721, 1741
BsaWI	WCCGGW	3 cut(s)	232, 253, 378
BsaXI	ACNNNNNCTCC	2 cut(s)	900, 930
Bsc4I	CCNNNNNNNGG	3 cut(s)	140, 647, 1538
Bse1I	ACTGG	8 cut(s)	97, 365, 389, 1305, 1543, 1553, 1773, 1775
Bse21I	CCTNAGG	1 cut(s)	847
Bse3DI	GCAATG	2 cut(s)	434, 1159
BseDI	CCNNGG	3 cut(s)	1642, 1721, 1741
BseGI	GGATG	3 cut(s)	598, 637, 1239
BseLI	CCNNNNNNNGG	3 cut(s)	140, 647, 1538
BseMI	GCAATG	2 cut(s)	434, 1159
BseMII	CTCAG	2 cut(s)	344, 861
BseNI	ACTGG	8 cut(s)	97, 365, 389, 1305, 1543, 1553, 1773, 1775
BseRI	GAGGAG	3 cut(s)	72, 485, 1631
BseXI	GCAGC	8 cut(s)	651, 869, 874, 1051, 1328, 1444, 1618, 1738
BseYI	CCCAGC	1 cut(s)	1376
BsgI	GTGCAG	1 cut(s)	1335
BshFI	GGCC	2 cut(s)	96, 1727
BsiSI	CCGG	5 cut(s)	233, 254, 379, 777, 1667
BslFI	GGGAC	3 cut(s)	784, 963, 1649
BslI	CCNNNNNNNGG	3 cut(s)	140, 647, 1538
BsmAI	GTCTC	2 cut(s)	1143, 1344
BsmFI	GGGAC	3 cut(s)	784, 963, 1649
BsmI	GAATGC	1 cut(s)	921
BsnI	GGCC	2 cut(s)	96, 1727
Bso31I	GGTCTC	1 cut(s)	1344
Bsp1286I	GDGCHC	2 cut(s)	222, 1115
Bsp143I	GATC	8 cut(s)	40, 337, 361, 416, 673, 817, 839, 1163
BspACI	CCGC	4 cut(s)	812, 837, 870, 1755
BspANI	GGCC	2 cut(s)	96, 1727
BspCNI	CTCAG	2 cut(s)	343, 860
BspHI	TCATGA	1 cut(s)	18
BspLI	GGNNCC	5 cut(s)	772, 799, 913, 1293, 1773
BspMI	ACCTGC	2 cut(s)	430, 1798
BspPI	GGATC	6 cut(s)	332, 356, 681, 812, 847, 1171
BspQI	GCTCTTC	1 cut(s)	210
BspTI	CTTAAG	1 cut(s)	1244
BspTNI	GGTCTC	1 cut(s)	1344
BsrDI	GCAATG	2 cut(s)	434, 1159
BsrI	ACTGG	8 cut(s)	97, 365, 389, 1305, 1543, 1553, 1773, 1775
BssECI	CCNNGG	3 cut(s)	1642, 1721, 1741
BssMI	GATC	8 cut(s)	40, 337, 361, 416, 673, 817, 839, 1163
BssT1I	CCWWGG	2 cut(s)	1721, 1741
Bst4CI	ACNGT	8 cut(s)	52, 205, 1009, 1119, 1268, 1350, 1370, 1738
Bst6I	CTCTTC	2 cut(s)	210, 1299
BstAFI	CTTAAG	1 cut(s)	1244
BstC8I	GCNNGC	4 cut(s)	353, 357, 540, 1314
BstDEI	CTNAG	5 cut(s)	330, 420, 847, 1394, 1469
BstF5I	GGATG	3 cut(s)	598, 637, 1239
BstHHI	GCGC	1 cut(s)	1620
BstKTI	GATC	8 cut(s)	43, 340, 364, 419, 676, 820, 842, 1166
BstMAI	GTCTC	2 cut(s)	1143, 1344
BstMBI	GATC	8 cut(s)	40, 337, 361, 416, 673, 817, 839, 1163
BstMWI	GCNNNNNNNGC	1 cut(s)	866
BstNSI	RCATGY	1 cut(s)	359
BstSCI	CCNGG	2 cut(s)	776, 1665
BstSFI	CTRYAG	3 cut(s)	294, 412, 1695
BstV1I	GCAGC	8 cut(s)	651, 869, 874, 1051, 1328, 1444, 1618, 1738
BstV2I	GAAGAC	5 cut(s)	317, 962, 1242, 1344, 1723
BstX2I	RGATCY	4 cut(s)	361, 416, 839, 1163
BstYI	RGATCY	4 cut(s)	361, 416, 839, 1163
Bsu36I	CCTNAGG	1 cut(s)	847
BsuRI	GGCC	2 cut(s)	96, 1727
BtsCI	GGATG	3 cut(s)	598, 637, 1239
BtsIMutI	CAGTG	4 cut(s)	372, 1014, 1346, 1560
BveI	ACCTGC	2 cut(s)	430, 1798
Cac8I	GCNNGC	4 cut(s)	353, 357, 540, 1314
CciI	TCATGA	1 cut(s)	18
CfoI	GCGC	1 cut(s)	1620
Cfr13I	GGNCC	3 cut(s)	771, 1022, 1725
Csp6I	GTAC	3 cut(s)	382, 829, 1410
CviQI	GTAC	3 cut(s)	382, 829, 1410
DdeI	CTNAG	5 cut(s)	330, 420, 847, 1394, 1469
DpnI	GATC	8 cut(s)	42, 339, 363, 418, 675, 819, 841, 1165
DpnII	GATC	8 cut(s)	40, 337, 361, 416, 673, 817, 839, 1163
DraIII	CACNNNGTG	1 cut(s)	938
DrdI	GACNNNNNNGTC	1 cut(s)	1517
DseDI	GACNNNNNNGTC	1 cut(s)	1517
Eam1104I	CTCTTC	2 cut(s)	210, 1299
EarI	CTCTTC	2 cut(s)	210, 1299
EciI	GGCGGA	1 cut(s)	852
Eco130I	CCWWGG	2 cut(s)	1721, 1741
Eco24I	GRGCYC	2 cut(s)	222, 1115
Eco31I	GGTCTC	1 cut(s)	1344
Eco47I	GGWCC	2 cut(s)	771, 1022
Eco57I	CTGAAG	4 cut(s)	564, 912, 974, 1428
Eco81I	CCTNAGG	1 cut(s)	847
EcoT14I	CCWWGG	2 cut(s)	1721, 1741
EcoT22I	ATGCAT	2 cut(s)	406, 756
EcoT38I	GRGCYC	2 cut(s)	222, 1115
ErhI	CCWWGG	2 cut(s)	1721, 1741
FalI	AAGNNNNNCTT	6 cut(s)	120, 152, 1227, 1259, 1674, 1706
FaqI	GGGAC	3 cut(s)	784, 963, 1649
FauI	CCCGC	1 cut(s)	819
FauNDI	CATATG	2 cut(s)	406, 964
FokI	GGATG	3 cut(s)	605, 624, 1226
FriOI	GRGCYC	2 cut(s)	222, 1115
FspBI	CTAG	3 cut(s)	893, 1175, 1628
GlaI	GCGC	1 cut(s)	1619
GsaI	CCCAGC	1 cut(s)	1380
GsuI	CTGGAG	2 cut(s)	1536, 1758
HaeIII	GGCC	2 cut(s)	96, 1727
HapII	CCGG	5 cut(s)	233, 254, 379, 777, 1667
HhaI	GCGC	1 cut(s)	1620
Hin6I	GCGC	1 cut(s)	1618
HinP1I	GCGC	1 cut(s)	1618
HindIII	AAGCTT	1 cut(s)	546
HinfI	GANTC	2 cut(s)	391, 692
HpaII	CCGG	5 cut(s)	233, 254, 379, 777, 1667
Hpy166II	GTNNAC	3 cut(s)	632, 831, 1025
Hpy188I	TCNGA	4 cut(s)	642, 972, 1225, 1447
Hpy188III	TCNNGA	6 cut(s)	19, 248, 395, 1040, 1295, 1565
Hpy8I	GTNNAC	3 cut(s)	632, 831, 1025
HpyAV	CCTTC	6 cut(s)	112, 351, 682, 936, 992, 1060
HpyCH4III	ACNGT	8 cut(s)	52, 205, 1009, 1119, 1268, 1350, 1370, 1738
HpyCH4IV	ACGT	2 cut(s)	25, 1512
HpyF10VI	GCNNNNNNNGC	1 cut(s)	866
HpyF3I	CTNAG	5 cut(s)	330, 420, 847, 1394, 1469
HpySE526I	ACGT	2 cut(s)	25, 1512
HspAI	GCGC	1 cut(s)	1618
Kzo9I	GATC	8 cut(s)	40, 337, 361, 416, 673, 817, 839, 1163
LguI	GCTCTTC	1 cut(s)	210
LmnI	GCTCC	5 cut(s)	85, 256, 1254, 1555, 1777
Lsp1109I	GCAGC	8 cut(s)	651, 869, 874, 1051, 1328, 1444, 1618, 1738
LweI	GCATC	4 cut(s)	508, 1036, 1299, 1558
MaeI	CTAG	3 cut(s)	893, 1175, 1628
MaeII	ACGT	2 cut(s)	25, 1512
MaeIII	GTNAC	5 cut(s)	604, 1214, 1525, 1600, 1777
MalI	GATC	8 cut(s)	42, 339, 363, 418, 675, 819, 841, 1165
MboI	GATC	8 cut(s)	40, 337, 361, 416, 673, 817, 839, 1163
MflI	RGATCY	4 cut(s)	361, 416, 839, 1163
MhlI	GDGCHC	2 cut(s)	222, 1115
MluCI	AATT	7 cut(s)	118, 784, 790, 1017, 1099, 1384, 1422
MmeI	TCCRAC	4 cut(s)	518, 665, 950, 1367
Mph1103I	ATGCAT	2 cut(s)	406, 756
MroXI	GAANNNNTTC	1 cut(s)	1502
MseI	TTAA	4 cut(s)	117, 1197, 1245, 1613
MspA1I	CMGCKG	3 cut(s)	698, 872, 887
MspCI	CTTAAG	1 cut(s)	1244
MspI	CCGG	5 cut(s)	233, 254, 379, 777, 1667
MspR9I	CCNGG	2 cut(s)	778, 1667
Mva1269I	GAATGC	1 cut(s)	921
MwoI	GCNNNNNNNGC	1 cut(s)	866
NciI	CCSGG	2 cut(s)	778, 1667
NdeI	CATATG	2 cut(s)	406, 964
NdeII	GATC	8 cut(s)	40, 337, 361, 416, 673, 817, 839, 1163
NlaIV	GGNNCC	5 cut(s)	772, 799, 913, 1293, 1773
NmeAIII	GCCGAG	1 cut(s)	1623
NmuCI	GTSAC	4 cut(s)	604, 1214, 1525, 1600
NsiI	ATGCAT	2 cut(s)	406, 756
NspI	RCATGY	1 cut(s)	359
PaeI	GCATGC	1 cut(s)	359
PagI	TCATGA	1 cut(s)	18
PciSI	GCTCTTC	1 cut(s)	210
PctI	GAATGC	1 cut(s)	921
PdmI	GAANNNNTTC	1 cut(s)	1502
PfeI	GAWTC	2 cut(s)	391, 692
PflFI	GACNNNGTC	1 cut(s)	985
PspFI	CCCAGC	1 cut(s)	1376
PspN4I	GGNNCC	5 cut(s)	772, 799, 913, 1293, 1773
PspPI	GGNCC	3 cut(s)	771, 1022, 1725
PsuI	RGATCY	4 cut(s)	361, 416, 839, 1163
PsyI	GACNNNGTC	1 cut(s)	985
PvuII	CAGCTG	2 cut(s)	698, 887
RsaI	GTAC	3 cut(s)	383, 830, 1411
RsaNI	GTAC	3 cut(s)	382, 829, 1410
SapI	GCTCTTC	1 cut(s)	210
SaqAI	TTAA	4 cut(s)	117, 1197, 1245, 1613
Sau3AI	GATC	8 cut(s)	40, 337, 361, 416, 673, 817, 839, 1163
Sau96I	GGNCC	3 cut(s)	771, 1022, 1725
ScrFI	CCNGG	2 cut(s)	778, 1667
SduI	GDGCHC	2 cut(s)	222, 1115
SfaNI	GCATC	4 cut(s)	508, 1036, 1299, 1558
SfcI	CTRYAG	3 cut(s)	294, 412, 1695
SinI	GGWCC	2 cut(s)	771, 1022
SmlI	CTYRAG	2 cut(s)	246, 1244
SmoI	CTYRAG	2 cut(s)	246, 1244
SphI	GCATGC	1 cut(s)	359
Sse9I	AATT	7 cut(s)	118, 784, 790, 1017, 1099, 1384, 1422
SsiI	CCGC	4 cut(s)	812, 837, 870, 1755
SspI	AATATT	2 cut(s)	600, 1594
SspMI	CTAG	3 cut(s)	893, 1175, 1628
StyD4I	CCNGG	2 cut(s)	776, 1665
StyI	CCWWGG	2 cut(s)	1721, 1741
TaaI	ACNGT	8 cut(s)	52, 205, 1009, 1119, 1268, 1350, 1370, 1738
TaiI	ACGT	2 cut(s)	28, 1515
TaqI	TCGA	2 cut(s)	1331, 1761
TasI	AATT	7 cut(s)	118, 784, 790, 1017, 1099, 1384, 1422
TauI	GCSGC	2 cut(s)	872, 1757
TfiI	GAWTC	2 cut(s)	391, 692
Tru1I	TTAA	4 cut(s)	117, 1197, 1245, 1613
Tru9I	TTAA	4 cut(s)	117, 1197, 1245, 1613
TscAI	CASTG	4 cut(s)	372, 1014, 1353, 1560
TseFI	GTSAC	4 cut(s)	604, 1214, 1525, 1600
TseI	GCWGC	8 cut(s)	664, 857, 887, 1064, 1316, 1457, 1631, 1751
Tsp45I	GTSAC	4 cut(s)	604, 1214, 1525, 1600
TspDTI	ATGAA	7 cut(s)	35, 89, 300, 540, 1023, 1071, 1422
TspGWI	ACGGA	5 cut(s)	571, 810, 904, 1161, 1418
TspRI	CASTG	4 cut(s)	372, 1014, 1353, 1560
Tth111I	GACNNNGTC	1 cut(s)	985
Vha464I	CTTAAG	1 cut(s)	1244
VpaK11BI	GGWCC	2 cut(s)	771, 1022
XapI	RAATTY	1 cut(s)	1384
XceI	RCATGY	1 cut(s)	359
XcmI	CCANNNNNNNNNTGG	1 cut(s)	644
XmnI	GAANNNNTTC	1 cut(s)	1502
XspI	CTAG	3 cut(s)	893, 1175, 1628
Zsp2I	ATGCAT	2 cut(s)	406, 756

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.