Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0005375

Physical Location & Seq

Genomic Coordinates Forward (+)

8713327 .. 8721284

Gene Structure 7958 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1050200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1824 bp

ATGGGGAAGAAGCGGGTGATGGTGGCCGCCGAGGATGTGGACTTGTCCGCCGTCAAATACGAGCAAGAAGAAATCAAGGCTCCACATTTGACTGGCTTGATTTTCAAGCTGTTTGTGAGGATTGTTGAGGCCCCCATCGTAGGTTCTTTCATACTCTCAGCTATGAAGAAGCAGAACAAGATGACTCAGTTGTTGAGGAATACTCTGATACCAGAGGGACCCATGTTCAAACCTGAATTTCCCCCTCAAGAACTAGAAGCCGGGGTGGTTGTTCTTGATGAAGATGGAAAACCAGAAGACAGAGTTGAAGTGGCTTTGAAATGCCTTCCACAGTATGATCCTGCTAGCTGCTGGAGTGGGAATTCAATTCAATCTTTTCGGTACTGGAGGATACGTGATTATGCTTATGCTTATCGTTCCAAGCTTGCAACCCCGTCACTGGTCGCGGAGCATGTAATTTCAGTTATAGATGAATTCAGTAATAAGAAACCACCACAACCTCTGTTGATTACTTTCAACCCTGAAGAAGTCAGGAGCCAAGCTGCAGCTTCGACAAAGAGGTTTGAGGAAGGGAATCCCTTATCCATCTTAGACGGGATTTTTGTGGCAATCAAGGATGATATAGACTGCTTTCCCCATCCATCTAAGGGTGGAACAACATGGATGCATGAGGTTCGCACTGTCAAGAAGGATGCAGTTTGTGTTTCGAGATTACGTAGTTGCGGCGTGATTTTCATTGGGAAGGCAAATATGCATGAGTTGGGCATGGGTACAACCGGAAATAATTCGAACTATGGAACAACAAGAAATCCCCATGCACCAGAAAGGTATACAGGTGGATCTTCATCGGGCCCTGCGGCACTTGTAGCTTCTGGACTGTGTTCTGCAGCGCTTGGAACTGATGGTGGAGGTTCAGTCCGTATTCCTTCTTCTCTGTGTGGTGTAGTGGGCTTGAAGACAACATATGGACGGACAGATATTGGAGGATCATTATGTGAGGATGGGACTGTGGAAATTATTGGACCAATTGCATCTACAGTGGAGGATGTCATGTTAGTGTACTCGGCAATTTTGGGCACCTCTCTTGCAGATAGACTTAGTTTGAAACCGTCCCCACCTTCTGTGCCAAATTTGTCATTGTATGACAGTTTGAATACTCTGGGATCCTTGCGACTGGGGAAGTATACAGCGTGGTTTAATGACGTACATTCGACTGATATCTCTGATACGTGTGAGGATGTTCTTAGTCTTCTATTGAAAACCCATGGTTGTGAAATGGTGGAGATTGTTGTACCTGAGCTCCATGAAATGCGTACTGCTCATCTCATTTCAATTGGATCTGAGTTTCTCAATGCACTGAATCCATATTGTGAGGAAGGATATGGTGCAAAATTGTCATATGACTCACGCACTAATGTGGCTCTTTTCCGATCATTTTCTGCGTCAGATTATATTGCTGCCCAATGTCTTAGGAGAAGGATTATGTACCATCATATGGAGATCTTCAAGAAGGTTGATGTCATAGTGACCCCCACAACAGGCATGACAGCACCTATAATTCCACCAGCTGCTCTTAAAGATGGGGAGACAGACATGCGGGTCACAGGTGATCTCATGCGGTTTGTCATTGCGGGAAATCTTCTTGGACTTCCTGCTATTTCTGTACCAGTTGGCTACGACAAGCAAGGGCTTCCAATCGGTTTGCAACTGGTTGGCCGCCCATGGGGTGAATCTTCAATTTTGCGTTTGGCTTCTGCAATAGAGGAACTCTGTGCCAAGTCTAAGAAGAGGCCTGTACAATATTTTGATGTTCTAAAGTCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005783 GO:0005794 GO:0005886 GO:0006066 GO:0006576 GO:0006807 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0009308 GO:0009605 GO:0009607 GO:0009617 GO:0009987 GO:0012505 GO:0016020 GO:0016787 GO:0016810 GO:0016811 GO:0031090 GO:0034308 GO:0034641 GO:0042439 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044106 GO:0044237 GO:0044281 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0047412 GO:0050896 GO:0051704 GO:0051707 GO:0070291 GO:0071704 GO:0071944 GO:0098542 GO:0098588 GO:0098805 GO:1901564 GO:1901615

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Amidase

Protein Analysis

608

Amino Acids

66.11

Weight (kDa)

6.22

Isoelectric Point (pI)

41.71

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Amidase	PF01425	169 - 581	1.6e-82	Amidase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005375)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G64440
fragaria_vesca	FvH4_2g11120 FvH4_2g11120 FvH4_2g11120 FvH4_2g11120 FvH4_2g11120 FvH4_2g11120 FvH4_2g11120 FvH4_2g11120 FvH4_2g11120
malus_domestica	MD05G1050200.v1.1
prunus_persica	Prupe.8G076300_v2.0.a1 Prupe.8G076300_v2.0.a1 Prupe.8G076300_v2.0.a1 Prupe.8G076300_v2.0.a1
pyrus_communis	pycom05g04210
rosa_chinensis	RchiOBHm_Chr6g0269381
rosa_laevigata	RLG00000013854
rosa_multiflora	Rmu_sc0003735.1_g000029 Rmu_sc0015769.1_g000001 Rmu_ssc0000291.1_g000010
rosa_roxburghii	Rroxscaffold_7G00198770
rosa_rugosa	Rorug06G0047400
rosa_samantha	Rh6AG167800 Rh6AG331500 Rh6BG173100 Rh6CG167200 Rh6DG159000
rosa_wichuraiana	Rw6G028780

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1598
AccB1I	GGYRCC	1 cut(s)	1076
AccB7I	CCANNNNNTGG	1 cut(s)	1495
AccI	GTMKAC	2 cut(s)	830, 1184
AccII	CGCG	1 cut(s)	446
AclWI	GGATC	6 cut(s)	332, 847, 994, 1158, 1171, 1345
AcoI	YGGCCR	2 cut(s)	24, 1714
AcsI	RAATTY	4 cut(s)	236, 361, 473, 1129
AcuI	CTGAAG	1 cut(s)	543
AfaI	GTAC	9 cut(s)	383, 772, 1061, 1206, 1293, 1315, 1487, 1665, 1797
AfeI	AGCGCT	1 cut(s)	891
AfiI	CCNNNNNNNGG	6 cut(s)	140, 439, 647, 1495, 1538, 1723
AflIII	ACRYGT	1 cut(s)	1229
AleI	CACNNNNGTG	1 cut(s)	1415
AluBI	AGCT	9 cut(s)	109, 161, 348, 424, 542, 548, 869, 1300, 1568
AluI	AGCT	9 cut(s)	109, 161, 348, 424, 542, 548, 869, 1300, 1568
Alw21I	GWGCWC	1 cut(s)	1302
Alw26I	GTCTC	1 cut(s)	1580
AlwI	GGATC	6 cut(s)	332, 847, 994, 1158, 1171, 1345
Aor51HI	AGCGCT	1 cut(s)	891
AoxI	GGCC	5 cut(s)	24, 129, 850, 1714, 1790
ApaI	GGGCCC	1 cut(s)	854
ApeKI	GCWGC	6 cut(s)	348, 542, 545, 887, 1457, 1568
ApoI	RAATTY	4 cut(s)	236, 361, 473, 1129
ArsI	GACNNNNNNTTYG	2 cut(s)	584, 616
Asp700I	GAANNNNTTC	1 cut(s)	784
AspLEI	GCGC	1 cut(s)	892
AspS9I	GGNCC	5 cut(s)	130, 218, 850, 851, 1022
AsuC2I	CCSGG	1 cut(s)	262
AsuHPI	GGTGA	3 cut(s)	28, 1619, 1739
AsuII	TTCGAA	1 cut(s)	788
AsuNHI	GCTAGC	1 cut(s)	344
AvaII	GGWCC	2 cut(s)	218, 1022
BaeGI	GKGCMC	2 cut(s)	854, 1079
BamHI	GGATCC	1 cut(s)	1163
BanI	GGYRCC	1 cut(s)	1076
BanII	GRGCYC	2 cut(s)	854, 1302
BarI	GAAGNNNNNNTAC	2 cut(s)	913, 945
BbsI	GAAGAC	3 cut(s)	303, 962, 1241
Bbv12I	GWGCWC	1 cut(s)	1302
BbvI	GCAGC	6 cut(s)	335, 529, 557, 899, 1444, 1555
BceAI	ACGGC	1 cut(s)	35
BcgI	CGANNNNNNTGC	2 cut(s)	656, 690
BciVI	GTATCC	1 cut(s)	384
BcnI	CCSGG	1 cut(s)	262
BcoDI	GTCTC	1 cut(s)	1580
BfaI	CTAG	2 cut(s)	254, 345
BfmI	CTRYAG	3 cut(s)	543, 885, 1035
BfoI	RGCGCY	1 cut(s)	893
BfuI	GTATCC	1 cut(s)	384
BglII	AGATCT	1 cut(s)	1500
Bme1390I	CCNGG	1 cut(s)	262
Bme18I	GGWCC	2 cut(s)	218, 1022
BmgT120I	GGNCC	5 cut(s)	130, 218, 850, 851, 1022
BmiI	GGNNCC	8 cut(s)	81, 132, 219, 220, 536, 852, 1078, 1165
BmrFI	CCNGG	1 cut(s)	262
BmrI	ACTGGG	1 cut(s)	1184
BmsI	GCATC	3 cut(s)	654, 682, 1040
BmtI	GCTAGC	1 cut(s)	348
BmuI	ACTGGG	1 cut(s)	1184
BpiI	GAAGAC	3 cut(s)	303, 962, 1241
BplI	GAGNNNNNCTC	2 cut(s)	187, 219
BpmI	CTGGAG	2 cut(s)	373, 406
Bpu10I	CCTNAGC	1 cut(s)	1296
Bpu14I	TTCGAA	1 cut(s)	788
BpuEI	CTTGAG	1 cut(s)	231
BpuMI	CCSGG	1 cut(s)	262
BsaAI	YACGTR	3 cut(s)	395, 716, 1230
BsaBI	GATNNNNATC	1 cut(s)	844
BsaJI	CCNNGG	4 cut(s)	30, 261, 1264, 1721
BsaWI	WCCGGW	1 cut(s)	776
BsaXI	ACNNNNNCTCC	6 cut(s)	379, 409, 900, 930, 1284, 1314
Bsc4I	CCNNNNNNNGG	6 cut(s)	140, 439, 647, 1495, 1538, 1723
Bse1I	ACTGG	6 cut(s)	97, 389, 444, 1179, 1667, 1713
Bse3DI	GCAATG	1 cut(s)	1626
Bse8I	GATNNNNATC	1 cut(s)	844
BseDI	CCNNGG	4 cut(s)	30, 261, 1264, 1721
BseGI	GGATG	8 cut(s)	40, 622, 637, 669, 697, 1006, 1051, 1243
BseJI	GATNNNNATC	1 cut(s)	844
BseLI	CCNNNNNNNGG	6 cut(s)	140, 439, 647, 1495, 1538, 1723
BseMI	GCAATG	1 cut(s)	1626
BseMII	CTCAG	4 cut(s)	171, 200, 1287, 1332
BseNI	ACTGG	6 cut(s)	97, 389, 444, 1179, 1667, 1713
BseSI	GKGCMC	2 cut(s)	854, 1079
BseXI	GCAGC	6 cut(s)	335, 529, 557, 899, 1444, 1555
Bsh1236I	CGCG	1 cut(s)	446
BshFI	GGCC	5 cut(s)	26, 131, 852, 1716, 1792
BshNI	GGYRCC	1 cut(s)	1076
BsiHKAI	GWGCWC	1 cut(s)	1302
BsiSI	CCGG	2 cut(s)	261, 777
BslFI	GGGAC	3 cut(s)	231, 1018, 1096
BslI	CCNNNNNNNGG	6 cut(s)	140, 439, 647, 1495, 1538, 1723
BsmAI	GTCTC	1 cut(s)	1580
BsmFI	GGGAC	3 cut(s)	231, 1018, 1096
BsnI	GGCC	5 cut(s)	26, 131, 852, 1716, 1792
Bsp119I	TTCGAA	1 cut(s)	788
Bsp120I	GGGCCC	1 cut(s)	850
Bsp1286I	GDGCHC	3 cut(s)	854, 1079, 1302
Bsp1407I	TGTACA	1 cut(s)	1795
Bsp143I	GATC	8 cut(s)	337, 839, 986, 1163, 1337, 1430, 1500, 1609
Bsp19I	CCATGG	2 cut(s)	1264, 1721
BspANI	GGCC	5 cut(s)	26, 131, 852, 1716, 1792
BspCNI	CTCAG	4 cut(s)	170, 199, 1288, 1333
BspFNI	CGCG	1 cut(s)	446
BspLI	GGNNCC	8 cut(s)	81, 132, 219, 220, 536, 852, 1078, 1165
BspMAI	CTGCAG	2 cut(s)	547, 889
BspOI	GCTAGC	1 cut(s)	348
BspPI	GGATC	6 cut(s)	332, 847, 994, 1158, 1171, 1345
BspT104I	TTCGAA	1 cut(s)	788
BspT107I	GGYRCC	1 cut(s)	1076
BsrDI	GCAATG	1 cut(s)	1626
BsrGI	TGTACA	1 cut(s)	1795
BsrI	ACTGG	6 cut(s)	97, 389, 444, 1179, 1667, 1713
BssECI	CCNNGG	4 cut(s)	30, 261, 1264, 1721
BssMI	GATC	8 cut(s)	337, 839, 986, 1163, 1337, 1430, 1500, 1609
BssNAI	GTATAC	2 cut(s)	831, 1185
BssT1I	CCWWGG	2 cut(s)	1264, 1721
Bst1107I	GTATAC	2 cut(s)	831, 1185
Bst4CI	ACNGT	7 cut(s)	333, 682, 879, 1009, 1039, 1110, 1148
Bst6I	CTCTTC	1 cut(s)	1781
BstAUI	TGTACA	1 cut(s)	1795
BstBAI	YACGTR	3 cut(s)	395, 716, 1230
BstBI	TTCGAA	1 cut(s)	788
BstC8I	GCNNGC	2 cut(s)	346, 426
BstDSI	CCRYGG	2 cut(s)	1264, 1721
BstF5I	GGATG	8 cut(s)	40, 622, 637, 669, 697, 1006, 1051, 1243
BstFNI	CGCG	1 cut(s)	446
BstH2I	RGCGCY	1 cut(s)	893
BstHHI	GCGC	1 cut(s)	892
BstKTI	GATC	8 cut(s)	340, 842, 989, 1166, 1340, 1433, 1503, 1612
BstMAI	GTCTC	1 cut(s)	1580
BstMBI	GATC	8 cut(s)	337, 839, 986, 1163, 1337, 1430, 1500, 1609
BstMWI	GCNNNNNNNGC	1 cut(s)	866
BstNSI	RCATGY	2 cut(s)	455, 1597
BstSCI	CCNGG	1 cut(s)	260
BstSFI	CTRYAG	3 cut(s)	543, 885, 1035
BstSLI	GKGCMC	2 cut(s)	854, 1079
BstSNI	TACGTA	1 cut(s)	716
BstUI	CGCG	1 cut(s)	446
BstV1I	GCAGC	6 cut(s)	335, 529, 557, 899, 1444, 1555
BstV2I	GAAGAC	3 cut(s)	303, 962, 1241
BstX2I	RGATCY	4 cut(s)	839, 1163, 1337, 1500
BstYI	RGATCY	4 cut(s)	839, 1163, 1337, 1500
BstZ17I	GTATAC	2 cut(s)	831, 1185
BsuI	GTATCC	1 cut(s)	384
BsuRI	GGCC	5 cut(s)	26, 131, 852, 1716, 1792
BtgI	CCRYGG	2 cut(s)	1264, 1721
BtsCI	GGATG	8 cut(s)	40, 622, 637, 669, 697, 1006, 1051, 1243
BtsIMutI	CAGTG	4 cut(s)	437, 678, 1044, 1355
Cac8I	GCNNGC	2 cut(s)	346, 426
CfoI	GCGC	1 cut(s)	892
Cfr13I	GGNCC	5 cut(s)	130, 218, 850, 851, 1022
CseI	GACGC	1 cut(s)	1431
Csp6I	GTAC	9 cut(s)	382, 771, 1060, 1205, 1292, 1314, 1486, 1664, 1796
CviQI	GTAC	9 cut(s)	382, 771, 1060, 1205, 1292, 1314, 1486, 1664, 1796
DpnI	GATC	8 cut(s)	339, 841, 988, 1165, 1339, 1432, 1502, 1611
DpnII	GATC	8 cut(s)	337, 839, 986, 1163, 1337, 1430, 1500, 1609
DrdI	GACNNNNNNGTC	1 cut(s)	1598
DseDI	GACNNNNNNGTC	1 cut(s)	1598
EaeI	YGGCCR	2 cut(s)	24, 1714
Eam1104I	CTCTTC	1 cut(s)	1781
EarI	CTCTTC	1 cut(s)	1781
EciI	GGCGGA	1 cut(s)	37
Ecl136II	GAGCTC	1 cut(s)	1300
Eco105I	TACGTA	1 cut(s)	716
Eco130I	CCWWGG	2 cut(s)	1264, 1721
Eco147I	AGGCCT	1 cut(s)	1792
Eco24I	GRGCYC	2 cut(s)	854, 1302
Eco32I	GATATC	1 cut(s)	1219
Eco47I	GGWCC	2 cut(s)	218, 1022
Eco47III	AGCGCT	1 cut(s)	891
Eco53kI	GAGCTC	1 cut(s)	1300
Eco57I	CTGAAG	1 cut(s)	543
EcoICRI	GAGCTC	1 cut(s)	1300
EcoO109I	RGGNCCY	3 cut(s)	130, 218, 851
EcoRI	GAATTC	2 cut(s)	361, 473
EcoRV	GATATC	1 cut(s)	1219
EcoT14I	CCWWGG	2 cut(s)	1264, 1721
EcoT22I	ATGCAT	2 cut(s)	669, 756
EcoT38I	GRGCYC	2 cut(s)	854, 1302
ErhI	CCWWGG	2 cut(s)	1264, 1721
FaqI	GGGAC	3 cut(s)	231, 1018, 1096
FauI	CCCGC	3 cut(s)	6, 1590, 1624
FauNDI	CATATG	3 cut(s)	964, 1399, 1494
FblI	GTMKAC	2 cut(s)	830, 1184
FokI	GGATG	8 cut(s)	47, 624, 629, 676, 704, 1013, 1058, 1250
FriOI	GRGCYC	2 cut(s)	854, 1302
FspBI	CTAG	2 cut(s)	254, 345
GlaI	GCGC	1 cut(s)	891
GsuI	CTGGAG	2 cut(s)	373, 406
HaeII	RGCGCY	1 cut(s)	893
HaeIII	GGCC	5 cut(s)	26, 131, 852, 1716, 1792
HapII	CCGG	2 cut(s)	261, 777
HgaI	GACGC	1 cut(s)	1431
HhaI	GCGC	1 cut(s)	892
Hin6I	GCGC	1 cut(s)	890
HinP1I	GCGC	1 cut(s)	890
HindIII	AAGCTT	1 cut(s)	422
HinfI	GANTC	5 cut(s)	184, 574, 1360, 1403, 1730
HpaII	CCGG	2 cut(s)	261, 777
HphI	GGTGA	3 cut(s)	28, 1619, 1739
Hpy166II	GTNNAC	4 cut(s)	40, 831, 1060, 1185
Hpy188I	TCNGA	5 cut(s)	207, 1225, 1342, 1430, 1447
Hpy188III	TCNNGA	7 cut(s)	248, 275, 532, 685, 708, 873, 1507
Hpy8I	GTNNAC	4 cut(s)	40, 831, 1060, 1185
HpyAV	CCTTC	9 cut(s)	335, 563, 682, 736, 936, 1128, 1370, 1470, 1504
HpyCH4III	ACNGT	7 cut(s)	333, 682, 879, 1009, 1039, 1110, 1148
HpyCH4IV	ACGT	4 cut(s)	394, 715, 1203, 1229
HpyF10VI	GCNNNNNNNGC	1 cut(s)	866
HpySE526I	ACGT	4 cut(s)	394, 715, 1203, 1229
HspAI	GCGC	1 cut(s)	890
KflI	GGGWCCC	1 cut(s)	218
Kzo9I	GATC	8 cut(s)	337, 839, 986, 1163, 1337, 1430, 1500, 1609
LmnI	GCTCC	4 cut(s)	85, 448, 534, 1305
Lsp1109I	GCAGC	6 cut(s)	335, 529, 557, 899, 1444, 1555
LweI	GCATC	3 cut(s)	654, 682, 1040
MaeI	CTAG	2 cut(s)	254, 345
MaeII	ACGT	4 cut(s)	394, 715, 1203, 1229
MaeIII	GTNAC	3 cut(s)	435, 1525, 1600
MalI	GATC	8 cut(s)	339, 841, 988, 1165, 1339, 1432, 1502, 1611
MboI	GATC	8 cut(s)	337, 839, 986, 1163, 1337, 1430, 1500, 1609
MfeI	CAATTG	2 cut(s)	1026, 1332
MflI	RGATCY	4 cut(s)	839, 1163, 1337, 1500
MhlI	GDGCHC	3 cut(s)	854, 1079, 1302
MlyI	GAGTC	2 cut(s)	178, 1397
Mph1103I	ATGCAT	2 cut(s)	669, 756
MroXI	GAANNNNTTC	1 cut(s)	784
MseI	TTAA	3 cut(s)	1197, 1575, 1822
MslI	CAYNNNNRTG	3 cut(s)	1055, 1269, 1415
MspA1I	CMGCKG	1 cut(s)	1568
MspI	CCGG	2 cut(s)	261, 777
MspR9I	CCNGG	1 cut(s)	262
MunI	CAATTG	2 cut(s)	1026, 1332
MvnI	CGCG	1 cut(s)	446
MwoI	GCNNNNNNNGC	1 cut(s)	866
NciI	CCSGG	1 cut(s)	262
NcoI	CCATGG	2 cut(s)	1264, 1721
NdeI	CATATG	3 cut(s)	964, 1399, 1494
NdeII	GATC	8 cut(s)	337, 839, 986, 1163, 1337, 1430, 1500, 1609
NheI	GCTAGC	1 cut(s)	344
NlaIV	GGNNCC	8 cut(s)	81, 132, 219, 220, 536, 852, 1078, 1165
NmeAIII	GCCGAG	2 cut(s)	55, 1043
NmuCI	GTSAC	3 cut(s)	435, 1525, 1600
NsiI	ATGCAT	2 cut(s)	669, 756
NspI	RCATGY	2 cut(s)	455, 1597
NspV	TTCGAA	1 cut(s)	788
OliI	CACNNNNGTG	1 cut(s)	1415
PceI	AGGCCT	1 cut(s)	1792
PdmI	GAANNNNTTC	1 cut(s)	784
PfeI	GAWTC	3 cut(s)	574, 1360, 1730
PflMI	CCANNNNNTGG	1 cut(s)	1495
PleI	GAGTC	2 cut(s)	178, 1397
PpsI	GAGTC	2 cut(s)	178, 1397
Ppu21I	YACGTR	3 cut(s)	395, 716, 1230
PpuMI	RGGWCCY	1 cut(s)	218
Psp124BI	GAGCTC	1 cut(s)	1302
Psp5II	RGGWCCY	1 cut(s)	218
PspN4I	GGNNCC	8 cut(s)	81, 132, 219, 220, 536, 852, 1078, 1165
PspOMI	GGGCCC	1 cut(s)	850
PspPI	GGNCC	5 cut(s)	130, 218, 850, 851, 1022
PspPPI	RGGWCCY	1 cut(s)	218
PstI	CTGCAG	2 cut(s)	547, 889
PsuI	RGATCY	4 cut(s)	839, 1163, 1337, 1500
PvuII	CAGCTG	1 cut(s)	1568
RsaI	GTAC	9 cut(s)	383, 772, 1061, 1206, 1293, 1315, 1487, 1665, 1797
RsaNI	GTAC	9 cut(s)	382, 771, 1060, 1205, 1292, 1314, 1486, 1664, 1796
RseI	CAYNNNNRTG	3 cut(s)	1055, 1269, 1415
SacI	GAGCTC	1 cut(s)	1302
SaqAI	TTAA	3 cut(s)	1197, 1575, 1822
Sau3AI	GATC	8 cut(s)	337, 839, 986, 1163, 1337, 1430, 1500, 1609
Sau96I	GGNCC	5 cut(s)	130, 218, 850, 851, 1022
SchI	GAGTC	2 cut(s)	178, 1397
ScrFI	CCNGG	1 cut(s)	262
SduI	GDGCHC	3 cut(s)	854, 1079, 1302
SfaNI	GCATC	3 cut(s)	654, 682, 1040
SfcI	CTRYAG	3 cut(s)	543, 885, 1035
SfuI	TTCGAA	1 cut(s)	788
SinI	GGWCC	2 cut(s)	218, 1022
SmiMI	CAYNNNNRTG	3 cut(s)	1055, 1269, 1415
SmlI	CTYRAG	1 cut(s)	246
SmoI	CTYRAG	1 cut(s)	246
SnaBI	TACGTA	1 cut(s)	716
SseBI	AGGCCT	1 cut(s)	1792
SspI	AATATT	1 cut(s)	1802
SspMI	CTAG	2 cut(s)	254, 345
SstI	GAGCTC	1 cut(s)	1302
StuI	AGGCCT	1 cut(s)	1792
StyD4I	CCNGG	1 cut(s)	260
StyI	CCWWGG	2 cut(s)	1264, 1721
TaaI	ACNGT	7 cut(s)	333, 682, 879, 1009, 1039, 1110, 1148
TaiI	ACGT	4 cut(s)	397, 718, 1206, 1232
TaqI	TCGA	4 cut(s)	551, 707, 788, 1211
TatI	WGTACW	2 cut(s)	1059, 1795
TauI	GCSGC	4 cut(s)	29, 726, 860, 1719
TfiI	GAWTC	3 cut(s)	574, 1360, 1730
Tru1I	TTAA	3 cut(s)	1197, 1575, 1822
Tru9I	TTAA	3 cut(s)	1197, 1575, 1822
TscAI	CASTG	4 cut(s)	444, 685, 1044, 1362
TseFI	GTSAC	3 cut(s)	435, 1525, 1600
TseI	GCWGC	6 cut(s)	348, 542, 545, 887, 1457, 1568
Tsp45I	GTSAC	3 cut(s)	435, 1525, 1600
TspDTI	ATGAA	7 cut(s)	139, 179, 294, 486, 724, 834, 1320
TspGWI	ACGGA	2 cut(s)	908, 985
TspRI	CASTG	4 cut(s)	444, 685, 1044, 1362
Van91I	CCANNNNNTGG	1 cut(s)	1495
VpaK11BI	GGWCC	2 cut(s)	218, 1022
XapI	RAATTY	4 cut(s)	236, 361, 473, 1129
XceI	RCATGY	2 cut(s)	455, 1597
XmiI	GTMKAC	2 cut(s)	830, 1184
XmnI	GAANNNNTTC	1 cut(s)	784
XspI	CTAG	2 cut(s)	254, 345
Zsp2I	ATGCAT	2 cut(s)	669, 756

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.