Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0008581

Physical Location & Seq

Genomic Coordinates Reverse (-)

25925173 .. 25928879

Gene Structure 3707 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT5G64860.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1731 bp

ATGTCGATTCTACTTAGGCCGTCGTCCTCTCCTTCACTCTGTTCTTCTCTCAAGCTTTTCCGATTATCCTCTCCGGATTCTTTAATCGACGCAGCTGTCCTCAGGAACAGGACAAAGCCGTCGCAGTCGTTTCGAATGGAGGTCGTTTCGAGTAATTCCACGTGTCTTTCTAGTATTAGCGTCGGTGAAGATTTTCCATCAGAATATGAGCAGTGGCTACCGGTTCCGGATCCAGAGAGCAGGAGAAGAGCTGGCGTTTTGCTACACCCGACGTCGTTTCGTGGTCCTCATGGCATTGGTGATCTCGGAGAAGAAGCCTTCCGGTTCATCGATTGGCTTCATTCTACTGGTTGCTCCGTTTGGCAGGTTCTTCCTCTTGTTCCTCCAGACGAAGGAGGATCTCCTTATGCAGGACAGGATGCAAATTGTGGGAACACATTGTTGATTTCTCTAGATGAGCTTGTGAAAGACGGCTTGTTAATCAAGGATGAGCTCCCACAACCAATTGATGCTGACTCTGTGAACTATCAGACTGCCAACAAGTTAAAGAGTCCCTTGATTACGAAGGCAGCAAAGAGGCTTATTGATGGCAATGGTGAACTGAAGAGCAAACTGCTAGATTTCCGTAACGACCCCTCTATATCATGTTGGCTTGAAGATGCTGCTTATTTTGCAGCTATTGACAATACTTTAAATGCATACAGTTGGTTTGAGTGGCCTGAACCACTTAAAAACCGTCATCTTTCTGCCTTGGAAGCTATATATGAAAGCCAAAAGGAGTTTATAGACTTGTTCATTGCTAAGCAATTTTTGTTCCAAAGGCAGTGGCAGAAAGTTCGTGAGTATGCACGGCGGCAAGGAGTTGATATAATGGGAGATATGCCCATTTATGTAGGATATCACAGTGCAGACGTTTGGGCAAATAAGAAACATTTCTTACTGAACAAGAAAGGCTTTCCTCTTCTTGTTAGCGGTGTTCCTCCTGACTTGTTCAGTGAAACTGGTCAACTGTGGGGCAGCCCTCTTTATGACTGGAAAGCAATGGAGAGTGACCAATATTCTTGGTGGGTTAATCGAATAAGACGCGCACAGGACTTGTATGACGAATGCAGGATTGATCACTTCAGAGGATTTGCAGGGTTTTGGGCGGTCCCTTCTGAAGCGAAAGTTGCCATGGTTGGACGATGGAAGGTAGGACCTGGAAAGTCATTATTTGATGCCATTTCCAAAGGCGTTGGGAAGATCAAAATCATAGCTGAAGATTTGGGAGTTATTACTAAAGATGTAGTTGAGCTGAGGAAATCTATCGGAGCACCTGGAATGGCCGTCCTCCAATTTGCTTTTGGAGGAGGCGCCGATAACCCACATTTACCTCACAATCATGAAGTAAACCAAGTTGTATACTCTGGAACTCATGACAACGACACTATTCGAGGCTGGTGGGACACTCTGGACCAAGAAGAAAAGTCTAAGGCAATGAAATACCTGTCGATAGCTGGAGAAGACGATATATCATGGTCAGTCATCCAAGCTGCATTCTCTTCAACCGCTCAAACCGCAATCATACCGATGCAAGACATTCTAGGACTTGGAAGTTCTGCCAGGATGAACACTCCAGCCACTGAGGTGGGGAATTGGGGTTGGAGGATTCCAAGTTCAACGAGCTTTGATAATCTTGAAACTGAATCTGACAGACTCAGAGATCTTTTGTCATTGTACGGACGGCTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000023 GO:0000025 GO:0000272 GO:0003674 GO:0003824 GO:0004133 GO:0004134 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0005976 GO:0005977 GO:0005982 GO:0005983 GO:0005984 GO:0005996 GO:0006006 GO:0006073 GO:0006091 GO:0006112 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009251 GO:0009311 GO:0009313 GO:0009501 GO:0009507 GO:0009536 GO:0009987 GO:0015980 GO:0016052 GO:0016740 GO:0016757 GO:0016758 GO:0019318 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044237 GO:0044238 GO:0044247 GO:0044248 GO:0044260 GO:0044262 GO:0044264 GO:0044275 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046352 GO:0055114 GO:0071704 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 map00500 map01100

Pfam Domains

Protein Families

Glyco_hydro_77

Protein Analysis

576

Amino Acids

64.41

Weight (kDa)

5.44

Isoelectric Point (pI)

42.66

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_77	PF02446	88 - 551	1.2e-149	4-alpha-glucanotransferase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0008581)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G64860
fragaria_vesca	FvH4_1g22230 FvH4_1g22230 FvH4_1g22230
malus_domestica	MD15G1333300.v1.1
prunus_persica	Prupe.6G173200_v2.0.a1 Prupe.6G173200_v2.0.a1 Prupe.6G173200_v2.0.a1 Prupe.6G173200_v2.0.a1 Prupe.6G173200_v2.0.a1
pyrus_communis	pycom15g29230
rosa_chinensis	RchiOBHm_Chr2g0116371
rosa_laevigata	RLG00000018263
rosa_multiflora	Rmu_sc0004191.1_g000073 Rmu_sc0037950.1_g000001 Rmu_ssc0000108.1_g000003
rosa_roxburghii	Rroxscaffold_2G00127280
rosa_rugosa	Rorug02G0206000
rosa_samantha	Rh2AG261500 Rh2BG272900 Rh2CG267900 Rh2DG269700
rosa_wichuraiana	Rw2G020520

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	95, 118
AatII	GACGTC	1 cut(s)	275
Acc36I	ACCTGC	1 cut(s)	355
AccB1I	GGYRCC	1 cut(s)	1352
AccBSI	CCGCTC	1 cut(s)	1550
AccI	GTMKAC	1 cut(s)	1401
AccII	CGCG	1 cut(s)	1086
AccIII	TCCGGA	2 cut(s)	73, 226
AciI	CCGC	5 cut(s)	853, 972, 1148, 1548, 1557
AclWI	GGATC	3 cut(s)	224, 237, 406
AcoI	YGGCCR	1 cut(s)	1323
AcuI	CTGAAG	4 cut(s)	623, 1108, 1179, 1278
AcvI	CACGTG	1 cut(s)	162
AcyI	GRCGYC	2 cut(s)	272, 1353
AfaI	GTAC	1 cut(s)	1718
AfiI	CCNNNNNNNGG	2 cut(s)	392, 410
AflIII	ACRYGT	1 cut(s)	161
AgeI	ACCGGT	1 cut(s)	220
AgsI	TTSAA	4 cut(s)	656, 1545, 1659, 1679
AjnI	CCWGG	3 cut(s)	1198, 1315, 1601
AleI	CACNNNNGTG	1 cut(s)	1625
AloI	GAACNNNNNNTCC	2 cut(s)	1639, 1671
Alw21I	GWGCWC	2 cut(s)	495, 1315
AlwI	GGATC	3 cut(s)	224, 237, 406
AlwNI	CAGNNNCTG	1 cut(s)	1622
Aor13HI	TCCGGA	2 cut(s)	73, 226
AoxI	GGCC	3 cut(s)	17, 716, 1323
ApeKI	GCWGC	6 cut(s)	92, 569, 662, 674, 1017, 1532
AsiGI	ACCGGT	1 cut(s)	220
Asp700I	GAANNNNTTC	2 cut(s)	192, 932
AspLEI	GCGC	2 cut(s)	1088, 1355
AspS9I	GGNCC	4 cut(s)	284, 1150, 1196, 1453
AsuHPI	GGTGA	3 cut(s)	197, 311, 608
AsuII	TTCGAA	1 cut(s)	133
AvaII	GGWCC	4 cut(s)	284, 1150, 1196, 1453
AxyI	CCTNAGG	1 cut(s)	101
BamHI	GGATCC	1 cut(s)	229
BanI	GGYRCC	1 cut(s)	1352
BanII	GRGCYC	1 cut(s)	495
BbrPI	CACGTG	1 cut(s)	162
BbsI	GAAGAC	1 cut(s)	1509
Bbv12I	GWGCWC	2 cut(s)	495, 1315
BbvCI	CCTCAGC	1 cut(s)	1295
BbvI	GCAGC	6 cut(s)	104, 581, 649, 686, 1029, 1519
BccI	CCATC	3 cut(s)	205, 581, 1179
BceAI	ACGGC	5 cut(s)	4, 103, 487, 866, 1310
BcgI	CGANNNNNNTGC	4 cut(s)	113, 147, 818, 852
BciT130I	CCWGG	3 cut(s)	1200, 1317, 1603
BclI	TGATCA	1 cut(s)	1117
BfaI	CTAG	4 cut(s)	171, 452, 617, 1583
BfoI	RGCGCY	1 cut(s)	1356
BfuAI	ACCTGC	1 cut(s)	355
BglII	AGATCT	1 cut(s)	1702
BisI	GCNGC	7 cut(s)	93, 570, 663, 675, 854, 1018, 1533
BlpI	GCTNAGC	1 cut(s)	801
BlsI	GCNGC	7 cut(s)	94, 571, 664, 676, 855, 1019, 1534
Bme1390I	CCNGG	3 cut(s)	1200, 1317, 1603
Bme18I	GGWCC	4 cut(s)	284, 1150, 1196, 1453
BmgT120I	GGNCC	4 cut(s)	284, 1150, 1196, 1453
BmiI	GGNNCC	4 cut(s)	225, 231, 1152, 1354
BmrFI	CCNGG	3 cut(s)	1200, 1317, 1603
BmsI	GCATC	5 cut(s)	409, 499, 649, 1207, 1560
BpiI	GAAGAC	1 cut(s)	1509
BpmI	CTGGAG	3 cut(s)	369, 1518, 1599
Bpu10I	CCTNAGC	1 cut(s)	1295
Bpu1102I	GCTNAGC	1 cut(s)	801
Bpu14I	TTCGAA	1 cut(s)	133
BpuEI	CTTGAG	1 cut(s)	35
Bsa29I	ATCGAT	1 cut(s)	330
BsaAI	YACGTR	1 cut(s)	162
BsaBI	GATNNNNATC	1 cut(s)	1247
BsaHI	GRCGYC	2 cut(s)	272, 1353
BsaJI	CCNNGG	2 cut(s)	750, 1173
BsaWI	WCCGGW	4 cut(s)	73, 220, 226, 321
Bsc4I	CCNNNNNNNGG	2 cut(s)	392, 410
Bse118I	RCCGGY	1 cut(s)	220
Bse1I	ACTGG	3 cut(s)	352, 1006, 1037
Bse21I	CCTNAGG	1 cut(s)	101
Bse3DI	GCAATG	4 cut(s)	598, 795, 1047, 1482
Bse8I	GATNNNNATC	1 cut(s)	1247
BseAI	TCCGGA	2 cut(s)	73, 226
BseBI	CCWGG	3 cut(s)	1200, 1317, 1603
BseCI	ATCGAT	1 cut(s)	330
BseDI	CCNNGG	2 cut(s)	750, 1173
BseGI	GGATG	4 cut(s)	424, 493, 1524, 1611
BseJI	GATNNNNATC	1 cut(s)	1247
BseLI	CCNNNNNNNGG	2 cut(s)	392, 410
BseMI	GCAATG	4 cut(s)	598, 795, 1047, 1482
BseMII	CTCAG	4 cut(s)	115, 1286, 1614, 1711
BseNI	ACTGG	3 cut(s)	352, 1006, 1037
BseRI	GAGGAG	1 cut(s)	1362
BseXI	GCAGC	6 cut(s)	104, 581, 649, 686, 1029, 1519
BsgI	GTGCAG	1 cut(s)	927
Bsh1236I	CGCG	1 cut(s)	1086
BshFI	GGCC	3 cut(s)	19, 718, 1325
BshNI	GGYRCC	1 cut(s)	1352
BshTI	ACCGGT	1 cut(s)	220
BshVI	ATCGAT	1 cut(s)	330
BsiHKAI	GWGCWC	2 cut(s)	495, 1315
BsiSI	CCGG	4 cut(s)	74, 221, 227, 322
BslFI	GGGAC	3 cut(s)	537, 1136, 1457
BslI	CCNNNNNNNGG	2 cut(s)	392, 410
BsmFI	GGGAC	3 cut(s)	537, 1136, 1457
BsmI	GAATGC	2 cut(s)	1112, 1535
BsnI	GGCC	3 cut(s)	19, 718, 1325
Bsp119I	TTCGAA	1 cut(s)	133
Bsp1286I	GDGCHC	2 cut(s)	495, 1315
Bsp13I	TCCGGA	2 cut(s)	73, 226
Bsp143I	GATC	6 cut(s)	229, 301, 398, 1117, 1242, 1702
Bsp1720I	GCTNAGC	1 cut(s)	801
Bsp19I	CCATGG	1 cut(s)	1173
BspACI	CCGC	5 cut(s)	853, 972, 1148, 1548, 1557
BspANI	GGCC	3 cut(s)	19, 718, 1325
BspCNI	CTCAG	4 cut(s)	114, 1287, 1615, 1710
BspDI	ATCGAT	1 cut(s)	330
BspEI	TCCGGA	2 cut(s)	73, 226
BspFNI	CGCG	1 cut(s)	1086
BspHI	TCATGA	2 cut(s)	1381, 1414
BspLI	GGNNCC	4 cut(s)	225, 231, 1152, 1354
BspMI	ACCTGC	1 cut(s)	355
BspPI	GGATC	3 cut(s)	224, 237, 406
BspQI	GCTCTTC	2 cut(s)	241, 599
BspT104I	TTCGAA	1 cut(s)	133
BspT107I	GGYRCC	1 cut(s)	1352
BsrBI	CCGCTC	1 cut(s)	1550
BsrDI	GCAATG	4 cut(s)	598, 795, 1047, 1482
BsrFI	RCCGGY	1 cut(s)	220
BsrI	ACTGG	3 cut(s)	352, 1006, 1037
BssAI	RCCGGY	1 cut(s)	220
BssECI	CCNNGG	2 cut(s)	750, 1173
BssMI	GATC	6 cut(s)	229, 301, 398, 1117, 1242, 1702
BssNAI	GTATAC	1 cut(s)	1402
BssNI	GRCGYC	2 cut(s)	272, 1353
BssT1I	CCWWGG	2 cut(s)	750, 1173
Bst1107I	GTATAC	1 cut(s)	1402
Bst2UI	CCWGG	3 cut(s)	1200, 1317, 1603
Bst4CI	ACNGT	4 cut(s)	704, 737, 905, 1011
Bst6I	CTCTTC	4 cut(s)	241, 599, 966, 1546
BstACI	GRCGYC	2 cut(s)	272, 1353
BstBAI	YACGTR	1 cut(s)	162
BstBI	TTCGAA	1 cut(s)	133
BstC8I	GCNNGC	1 cut(s)	253
BstDEI	CTNAG	7 cut(s)	14, 101, 801, 1295, 1470, 1623, 1697
BstDSI	CCRYGG	1 cut(s)	1173
BstENI	CCTNNNNNAGG	1 cut(s)	408
BstF5I	GGATG	4 cut(s)	424, 493, 1524, 1611
BstFNI	CGCG	1 cut(s)	1086
BstH2I	RGCGCY	1 cut(s)	1356
BstHHI	GCGC	2 cut(s)	1088, 1355
BstKTI	GATC	6 cut(s)	232, 304, 401, 1120, 1245, 1705
BstMBI	GATC	6 cut(s)	229, 301, 398, 1117, 1242, 1702
BstMWI	GCNNNNNNNGC	4 cut(s)	671, 755, 1169, 1556
BstNI	CCWGG	3 cut(s)	1200, 1317, 1603
BstSCI	CCNGG	3 cut(s)	1198, 1315, 1601
BstUI	CGCG	1 cut(s)	1086
BstV1I	GCAGC	6 cut(s)	104, 581, 649, 686, 1029, 1519
BstV2I	GAAGAC	1 cut(s)	1509
BstX2I	RGATCY	3 cut(s)	229, 398, 1702
BstXI	CCANNNNNNTGG	1 cut(s)	1627
BstYI	RGATCY	3 cut(s)	229, 398, 1702
BstZ17I	GTATAC	1 cut(s)	1402
Bsu15I	ATCGAT	1 cut(s)	330
Bsu36I	CCTNAGG	1 cut(s)	101
BsuRI	GGCC	3 cut(s)	19, 718, 1325
BsuTUI	ATCGAT	1 cut(s)	330
BtgI	CCRYGG	1 cut(s)	1173
BtsCI	GGATG	4 cut(s)	424, 493, 1524, 1611
BtsI	GCAGTG	2 cut(s)	218, 830
BtsIMutI	CAGTG	5 cut(s)	218, 830, 910, 1000, 1620
BveI	ACCTGC	1 cut(s)	355
Cac8I	GCNNGC	1 cut(s)	253
CaiI	CAGNNNCTG	1 cut(s)	1622
CciI	TCATGA	2 cut(s)	1381, 1414
CfoI	GCGC	2 cut(s)	1088, 1355
Cfr10I	RCCGGY	1 cut(s)	220
Cfr13I	GGNCC	4 cut(s)	284, 1150, 1196, 1453
ClaI	ATCGAT	1 cut(s)	330
CseI	GACGC	3 cut(s)	98, 169, 1092
Csp6I	GTAC	1 cut(s)	1717
CspAI	ACCGGT	1 cut(s)	220
CspCI	CAANNNNNGTGG	2 cut(s)	806, 841
CviAII	CATG	6 cut(s)	290, 645, 1174, 1382, 1415, 1515
CviQI	GTAC	1 cut(s)	1717
DdeI	CTNAG	7 cut(s)	14, 101, 801, 1295, 1470, 1623, 1697
DinI	GGCGCC	1 cut(s)	1354
DpnI	GATC	6 cut(s)	231, 303, 400, 1119, 1244, 1704
DpnII	GATC	6 cut(s)	229, 301, 398, 1117, 1242, 1702
DraI	TTTAAA	1 cut(s)	693
DrdI	GACNNNNNNGTC	2 cut(s)	95, 118
DseDI	GACNNNNNNGTC	2 cut(s)	95, 118
EaeI	YGGCCR	1 cut(s)	1323
Eam1104I	CTCTTC	4 cut(s)	241, 599, 966, 1546
EarI	CTCTTC	4 cut(s)	241, 599, 966, 1546
Ecl136II	GAGCTC	1 cut(s)	493
Eco130I	CCWWGG	2 cut(s)	750, 1173
Eco24I	GRGCYC	1 cut(s)	495
Eco32I	GATATC	1 cut(s)	899
Eco47I	GGWCC	4 cut(s)	284, 1150, 1196, 1453
Eco53kI	GAGCTC	1 cut(s)	493
Eco57I	CTGAAG	4 cut(s)	623, 1108, 1179, 1278
Eco72I	CACGTG	1 cut(s)	162
Eco81I	CCTNAGG	1 cut(s)	101
EcoICRI	GAGCTC	1 cut(s)	493
EcoNI	CCTNNNNNAGG	1 cut(s)	408
EcoO109I	RGGNCCY	1 cut(s)	1196
EcoRII	CCWGG	3 cut(s)	1198, 1315, 1601
EcoRV	GATATC	1 cut(s)	899
EcoT14I	CCWWGG	2 cut(s)	750, 1173
EcoT22I	ATGCAT	1 cut(s)	700
EcoT38I	GRGCYC	1 cut(s)	495
EgeI	GGCGCC	1 cut(s)	1354
EheI	GGCGCC	1 cut(s)	1354
ErhI	CCWWGG	2 cut(s)	750, 1173
FaeI	CATG	6 cut(s)	293, 648, 1177, 1385, 1418, 1518
FalI	AAGNNNNNCTT	4 cut(s)	539, 571, 938, 970
FaqI	GGGAC	3 cut(s)	537, 1136, 1457
FatI	CATG	6 cut(s)	289, 644, 1173, 1381, 1414, 1514
FbaI	TGATCA	1 cut(s)	1117
FblI	GTMKAC	1 cut(s)	1401
Fnu4HI	GCNGC	7 cut(s)	93, 570, 663, 675, 854, 1018, 1533
FokI	GGATG	4 cut(s)	431, 500, 1511, 1618
FriOI	GRGCYC	1 cut(s)	495
Fsp4HI	GCNGC	7 cut(s)	93, 570, 663, 675, 854, 1018, 1533
FspBI	CTAG	4 cut(s)	171, 452, 617, 1583
GlaI	GCGC	2 cut(s)	1087, 1354
GluI	GCNGC	7 cut(s)	93, 570, 663, 675, 854, 1018, 1533
GsuI	CTGGAG	3 cut(s)	369, 1518, 1599
HaeII	RGCGCY	1 cut(s)	1356
HaeIII	GGCC	3 cut(s)	19, 718, 1325
HapII	CCGG	4 cut(s)	74, 221, 227, 322
HgaI	GACGC	3 cut(s)	98, 169, 1092
HhaI	GCGC	2 cut(s)	1088, 1355
Hin1I	GRCGYC	2 cut(s)	272, 1353
Hin1II	CATG	6 cut(s)	293, 648, 1177, 1385, 1418, 1518
Hin6I	GCGC	2 cut(s)	1086, 1353
HinP1I	GCGC	2 cut(s)	1086, 1353
HincII	GTYRAC	1 cut(s)	1007
HindII	GTYRAC	1 cut(s)	1007
HindIII	AAGCTT	1 cut(s)	53
HinfI	GANTC	7 cut(s)	7, 77, 515, 550, 1648, 1685, 1695
HpaII	CCGG	4 cut(s)	74, 221, 227, 322
HphI	GGTGA	3 cut(s)	197, 311, 608
Hpy166II	GTNNAC	5 cut(s)	523, 599, 1007, 1390, 1402
Hpy188I	TCNGA	9 cut(s)	62, 202, 308, 531, 1127, 1159, 1310, 1690, 1700
Hpy8I	GTNNAC	5 cut(s)	523, 599, 1007, 1390, 1402
Hpy99I	CGWCG	6 cut(s)	25, 92, 124, 185, 274, 277
HpyAV	CCTTC	6 cut(s)	42, 328, 386, 559, 1164, 1183
HpyCH4III	ACNGT	4 cut(s)	704, 737, 905, 1011
HpyCH4IV	ACGT	3 cut(s)	161, 272, 912
HpyF10VI	GCNNNNNNNGC	4 cut(s)	671, 755, 1169, 1556
HpyF3I	CTNAG	7 cut(s)	14, 101, 801, 1295, 1470, 1623, 1697
HpySE526I	ACGT	3 cut(s)	161, 272, 912
Hsp92I	GRCGYC	2 cut(s)	272, 1353
Hsp92II	CATG	6 cut(s)	293, 648, 1177, 1385, 1418, 1518
HspAI	GCGC	2 cut(s)	1086, 1353
KasI	GGCGCC	1 cut(s)	1352
Kpn2I	TCCGGA	2 cut(s)	73, 226
Ksp22I	TGATCA	1 cut(s)	1117
Kzo9I	GATC	6 cut(s)	229, 301, 398, 1117, 1242, 1702
LguI	GCTCTTC	2 cut(s)	241, 599
LmnI	GCTCC	3 cut(s)	359, 498, 1310
Lsp1109I	GCAGC	6 cut(s)	104, 581, 649, 686, 1029, 1519
LweI	GCATC	5 cut(s)	409, 499, 649, 1207, 1560
MaeI	CTAG	4 cut(s)	171, 452, 617, 1583
MaeII	ACGT	3 cut(s)	161, 272, 912
MaeIII	GTNAC	2 cut(s)	626, 1049
MalI	GATC	6 cut(s)	231, 303, 400, 1119, 1244, 1704
MbiI	CCGCTC	1 cut(s)	1550
MboI	GATC	6 cut(s)	229, 301, 398, 1117, 1242, 1702
MfeI	CAATTG	1 cut(s)	504
MflI	RGATCY	3 cut(s)	229, 398, 1702
MhlI	GDGCHC	2 cut(s)	495, 1315
MluCI	AATT	6 cut(s)	154, 424, 504, 806, 1334, 1633
Mly113I	GGCGCC	1 cut(s)	1353
MlyI	GAGTC	3 cut(s)	509, 559, 1689
MmeI	TCCRAC	2 cut(s)	1159, 1622
Mph1103I	ATGCAT	1 cut(s)	700
MroI	TCCGGA	2 cut(s)	73, 226
MroXI	GAANNNNTTC	2 cut(s)	192, 932
MseI	TTAA	6 cut(s)	83, 479, 545, 692, 729, 1071
MslI	CAYNNNNRTG	3 cut(s)	1380, 1568, 1625
MspA1I	CMGCKG	1 cut(s)	95
MspI	CCGG	4 cut(s)	74, 221, 227, 322
MspR9I	CCNGG	3 cut(s)	1200, 1317, 1603
MunI	CAATTG	1 cut(s)	504
Mva1269I	GAATGC	2 cut(s)	1112, 1535
MvaI	CCWGG	3 cut(s)	1200, 1317, 1603
MvnI	CGCG	1 cut(s)	1086
MwoI	GCNNNNNNNGC	4 cut(s)	671, 755, 1169, 1556
NarI	GGCGCC	1 cut(s)	1353
NcoI	CCATGG	1 cut(s)	1173
NdeII	GATC	6 cut(s)	229, 301, 398, 1117, 1242, 1702
NlaIII	CATG	6 cut(s)	293, 648, 1177, 1385, 1418, 1518
NlaIV	GGNNCC	4 cut(s)	225, 231, 1152, 1354
NmuCI	GTSAC	1 cut(s)	1049
NsiI	ATGCAT	1 cut(s)	700
NspV	TTCGAA	1 cut(s)	133
OliI	CACNNNNGTG	1 cut(s)	1625
PagI	TCATGA	2 cut(s)	1381, 1414
PciSI	GCTCTTC	2 cut(s)	241, 599
PctI	GAATGC	2 cut(s)	1112, 1535
PdmI	GAANNNNTTC	2 cut(s)	192, 932
PfeI	GAWTC	4 cut(s)	7, 77, 1648, 1685
PinAI	ACCGGT	1 cut(s)	220
PkrI	GCNGC	7 cut(s)	94, 571, 664, 676, 855, 1019, 1534
PleI	GAGTC	3 cut(s)	509, 558, 1689
PluTI	GGCGCC	1 cut(s)	1356
PmaCI	CACGTG	1 cut(s)	162
PmlI	CACGTG	1 cut(s)	162
PpsI	GAGTC	3 cut(s)	509, 558, 1689
Ppu21I	YACGTR	1 cut(s)	162
PpuMI	RGGWCCY	1 cut(s)	1196
Psp124BI	GAGCTC	1 cut(s)	495
Psp5II	RGGWCCY	1 cut(s)	1196
Psp6I	CCWGG	3 cut(s)	1198, 1315, 1601
PspCI	CACGTG	1 cut(s)	162
PspGI	CCWGG	3 cut(s)	1198, 1315, 1601
PspN4I	GGNNCC	4 cut(s)	225, 231, 1152, 1354
PspPI	GGNCC	4 cut(s)	284, 1150, 1196, 1453
PspPPI	RGGWCCY	1 cut(s)	1196
PstNI	CAGNNNCTG	1 cut(s)	1622
PsuI	RGATCY	3 cut(s)	229, 398, 1702
PvuII	CAGCTG	1 cut(s)	95
RsaI	GTAC	1 cut(s)	1718
RsaNI	GTAC	1 cut(s)	1717
RseI	CAYNNNNRTG	3 cut(s)	1380, 1568, 1625
SacI	GAGCTC	1 cut(s)	495
SapI	GCTCTTC	2 cut(s)	241, 599
SaqAI	TTAA	6 cut(s)	83, 479, 545, 692, 729, 1071
SatI	GCNGC	7 cut(s)	93, 570, 663, 675, 854, 1018, 1533
Sau3AI	GATC	6 cut(s)	229, 301, 398, 1117, 1242, 1702
Sau96I	GGNCC	4 cut(s)	284, 1150, 1196, 1453
SchI	GAGTC	3 cut(s)	509, 559, 1689
ScrFI	CCNGG	3 cut(s)	1200, 1317, 1603
SduI	GDGCHC	2 cut(s)	495, 1315
SfaNI	GCATC	5 cut(s)	409, 499, 649, 1207, 1560
SfoI	GGCGCC	1 cut(s)	1354
SfuI	TTCGAA	1 cut(s)	133
SinI	GGWCC	4 cut(s)	284, 1150, 1196, 1453
SmiMI	CAYNNNNRTG	3 cut(s)	1380, 1568, 1625
SmlI	CTYRAG	1 cut(s)	50
SmoI	CTYRAG	1 cut(s)	50
Sse9I	AATT	6 cut(s)	154, 424, 504, 806, 1334, 1633
SsiI	CCGC	5 cut(s)	853, 972, 1148, 1548, 1557
SspDI	GGCGCC	1 cut(s)	1352
SspI	AATATT	1 cut(s)	1058
SspMI	CTAG	4 cut(s)	171, 452, 617, 1583
SstI	GAGCTC	1 cut(s)	495
StyD4I	CCNGG	3 cut(s)	1198, 1315, 1601
StyI	CCWWGG	2 cut(s)	750, 1173
TaaI	ACNGT	4 cut(s)	704, 737, 905, 1011
TaiI	ACGT	3 cut(s)	164, 275, 915
TaqI	TCGA	8 cut(s)	5, 87, 133, 149, 330, 1075, 1432, 1490
TasI	AATT	6 cut(s)	154, 424, 504, 806, 1334, 1633
TauI	GCSGC	1 cut(s)	856
TfiI	GAWTC	4 cut(s)	7, 77, 1648, 1685
Tru1I	TTAA	6 cut(s)	83, 479, 545, 692, 729, 1071
Tru9I	TTAA	6 cut(s)	83, 479, 545, 692, 729, 1071
TscAI	CASTG	5 cut(s)	218, 830, 910, 1000, 1627
TseFI	GTSAC	1 cut(s)	1049
TseI	GCWGC	6 cut(s)	92, 569, 662, 674, 1017, 1532
Tsp45I	GTSAC	1 cut(s)	1049
TspDTI	ATGAA	7 cut(s)	316, 329, 780, 784, 1398, 1493, 1622
TspGWI	ACGGA	2 cut(s)	346, 614
TspRI	CASTG	5 cut(s)	218, 830, 910, 1000, 1627
VpaK11BI	GGWCC	4 cut(s)	284, 1150, 1196, 1453
XagI	CCTNNNNNAGG	1 cut(s)	408
XbaI	TCTAGA	1 cut(s)	451
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1340
XmiI	GTMKAC	1 cut(s)	1401
XmnI	GAANNNNTTC	2 cut(s)	192, 932
XspI	CTAG	4 cut(s)	171, 452, 617, 1583
ZraI	GACGTC	1 cut(s)	273
Zsp2I	ATGCAT	1 cut(s)	700

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.