AT5G64920

zinc-ribbon

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0014025
Physical Location & Seq
Reverse (-)
25943888 .. 25945442
1555 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G64920.1

Sequence Viewer

Length: 1005 bp
ATGTCCGATGCTCCGTCGTCTTCCCCGGATGCCACGGCGTCGCACTGGTGCTATCACTGCAACAAACGCGTCGTCGTTGAAACCTTAGATGACTTTGTCGTGTGCTGCGAATGTAACAAAGGTTTCGTCGAGTCAATTCAACCGACTCCCGCCGCTTATTCATCGCCGGCGCCACCGCAGCCACTTTCCCCAGATCTGAATGTAGAAGACTCCAGTATTGGCTCGCATTTCCTCCAGATGCTCCGCTTGTTAGCCCACGCGCCTTCTCAGCGTTCACCACCACGACACCTTGATGTTTTATCTTACGAAGATGATTTCTTCAGGTTGGAGCTCAATAGTAGAAACGAAATCGACGATGACGAAGACGAAGATGAAGATGATGGAGATGAAGAAGAAGAGGATGAGGAAGAGAATTTAACCGTCAACGACGAAGAAGACGAAGAAGATGATCTGAGGAGGAGAAATCGTTTTCCTCTCACGACGACGCAGTCGAGAACCGGAAGAAACAGAATTCTCGATTGGGCTGAGATTTTGATGGGAATCGAAGACAATTCGATTGAGTTCCGTATGGAATCAGATCGATACGCAGGAAATCCGGCTGATTACATAGACGATGCAGCCGGATACGAAGCTTTGCTACAGAATTTAGCAGAAGGAGATGGTGGTGGTGGCGGAGGAAGGAGAGGCGCACCACCGGCTGCGAAATCGGCAATAGAGGCATTGGAGACTTTCGAGGTTAGTTCTTCGGAGGGAGAGATGGTTATGGTTTGTGCTGTGTGTAAAGATGGAATGGTGATGGGAGAAACTGGTAAGAAGTTACCGTGTGGACATTGTTACCACGGAGATTGTATTGTGCCATGGTTAGGAACAAGGAACTCTTGTCCTGTCTGTAGATTCCAGCTTGAGACTGATGATGCTGAATATGAGGAAGAGAGGAAAAAAAGAACTTCTACCGTGTCAGATTCTGCTGCTGCTTCTTCTTCTTCTTCAACTTCTCGTTACTGA

Protein Analysis

334

Amino Acids

36.96

Weight (kDa)

4.29

Isoelectric Point (pI)

74.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zn_ribbon_19 PF14369 14 - 46 2.9e-10 zinc-ribbon
zf-RING_2 PF13639 257 - 298 2.8e-11 Ring finger domain
zf-C3HC4_2 PF13923 257 - 297 3.4e-08 Zinc finger, C3HC4 type (RING finger)
zf-RING_11 PF17123 257 - 284 2.6e-06 RING-like zinc finger
zf-rbx1 PF12678 274 - 298 5.6e-06 RING-H2 zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014025)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G64920
fragaria_vesca FvH4_2g38280
malus_domestica MD08G1011000.v1.1 MD15G1011000.v1.1
prunus_persica Prupe.1G363700_v2.0.a1
pyrus_communis pycom15g00940
rosa_chinensis RchiOBHm_Chr6g0303101
rosa_laevigata RLG00000011108
rosa_multiflora Rmu_sc0003721.1_g000001 Rmu_sc0008911.1_g000004
rosa_roxburghii Rroxscaffold_7G00165020
rosa_rugosa Rorug06G0319200
rosa_samantha Rh6AG433000 Rh6BG469700 Rh6CG445300 Rh6DG432100
rosa_wichuraiana Rw6G037530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 487
AccB1I GGYRCC 1 cut(s) 169
AccII CGCG 2 cut(s) 69, 260
AciI CCGC 5 cut(s) 150, 153, 176, 244, 672
AcsI RAATTY 3 cut(s) 412, 510, 643
AcuI CTGAAG 1 cut(s) 304
AcyI GRCGYC 2 cut(s) 38, 170
AfiI CCNNNNNNNGG 1 cut(s) 863
AflIII ACRYGT 1 cut(s) 67
AgsI TTSAA 3 cut(s) 80, 140, 990
AjuI GAANNNNNNNTTGG 2 cut(s) 502, 534
AluBI AGCT 3 cut(s) 331, 632, 901
AluI AGCT 3 cut(s) 331, 632, 901
Alw21I GWGCWC 1 cut(s) 333
Alw26I GTCTC 2 cut(s) 719, 899
AlwNI CAGNNNCTG 1 cut(s) 965
ApeKI GCWGC 6 cut(s) 105, 178, 617, 698, 968, 971
ApoI RAATTY 3 cut(s) 412, 510, 643
ArsI GACNNNNNNTTYG 4 cut(s) 57, 89, 111, 143
AspLEI GCGC 3 cut(s) 172, 262, 689
AsuC2I CCSGG 1 cut(s) 26
AsuHPI GGTGA 2 cut(s) 267, 805
BanI GGYRCC 1 cut(s) 169
BanII GRGCYC 1 cut(s) 333
BarI GAAGNNNNNNTAC 2 cut(s) 621, 653
BbsI GAAGAC 5 cut(s) 12, 213, 369, 441, 552
Bbv12I GWGCWC 1 cut(s) 333
BbvI GCAGC 6 cut(s) 92, 190, 629, 685, 955, 958
BccI CCATC 6 cut(s) 374, 529, 653, 751, 779, 790
BceAI ACGGC 1 cut(s) 51
BciVI GTATCC 1 cut(s) 617
BcnI CCSGG 1 cut(s) 26
BcoDI GTCTC 2 cut(s) 719, 899
BfmI CTRYAG 2 cut(s) 638, 889
BfoI RGCGCY 1 cut(s) 173
BfuI GTATCC 1 cut(s) 617
BglII AGATCT 1 cut(s) 193
BisI GCNGC 7 cut(s) 106, 153, 179, 618, 699, 969, 972
BlsI GCNGC 7 cut(s) 107, 154, 180, 619, 700, 970, 973
Bme1390I CCNGG 1 cut(s) 26
BmiI GGNNCC 1 cut(s) 171
BmrFI CCNGG 1 cut(s) 26
BmsI GCATC 4 cut(s) 19, 228, 604, 904
BpiI GAAGAC 5 cut(s) 12, 213, 369, 441, 552
BpmI CTGGAG 2 cut(s) 196, 218
BpuEI CTTGAG 1 cut(s) 923
BpuMI CCSGG 1 cut(s) 26
Bsa29I ATCGAT 1 cut(s) 580
BsaBI GATNNNNATC 1 cut(s) 539
BsaHI GRCGYC 2 cut(s) 38, 170
BsaJI CCNNGG 4 cut(s) 24, 33, 838, 857
BsaWI WCCGGW 1 cut(s) 497
BsaXI ACNNNNNCTCC 4 cut(s) 451, 481, 744, 774
Bsc4I CCNNNNNNNGG 1 cut(s) 863
Bse118I RCCGGY 2 cut(s) 166, 694
Bse1I ACTGG 3 cut(s) 50, 213, 811
Bse8I GATNNNNATC 1 cut(s) 539
BseCI ATCGAT 1 cut(s) 580
BseDI CCNNGG 4 cut(s) 24, 33, 838, 857
BseGI GGATG 2 cut(s) 34, 406
BseJI GATNNNNATC 1 cut(s) 539
BseLI CCNNNNNNNGG 1 cut(s) 863
BseMII CTCAG 3 cut(s) 281, 443, 516
BseNI ACTGG 3 cut(s) 50, 213, 811
BseRI GAGGAG 2 cut(s) 469, 472
BseXI GCAGC 6 cut(s) 92, 190, 629, 685, 955, 958
Bsh1236I CGCG 2 cut(s) 69, 260
BshNI GGYRCC 1 cut(s) 169
BshVI ATCGAT 1 cut(s) 580
BsiHKAI GWGCWC 1 cut(s) 333
BsiSI CCGG 6 cut(s) 26, 167, 498, 596, 621, 695
BslI CCNNNNNNNGG 1 cut(s) 863
BsmAI GTCTC 2 cut(s) 719, 899
Bsp1286I GDGCHC 1 cut(s) 333
Bsp143I GATC 3 cut(s) 193, 448, 577
Bsp19I CCATGG 1 cut(s) 857
BspACI CCGC 5 cut(s) 150, 153, 176, 244, 672
BspCNI CTCAG 3 cut(s) 280, 444, 517
BspDI ATCGAT 1 cut(s) 580
BspFNI CGCG 2 cut(s) 69, 260
BspLI GGNNCC 1 cut(s) 171
BspT107I GGYRCC 1 cut(s) 169
BsrFI RCCGGY 2 cut(s) 166, 694
BsrI ACTGG 3 cut(s) 50, 213, 811
BssAI RCCGGY 2 cut(s) 166, 694
BssECI CCNNGG 4 cut(s) 24, 33, 838, 857
BssMI GATC 3 cut(s) 193, 448, 577
BssNI GRCGYC 2 cut(s) 38, 170
BssT1I CCWWGG 1 cut(s) 857
Bst4CI ACNGT 3 cut(s) 421, 822, 955
Bst6I CTCTTC 3 cut(s) 390, 402, 924
BstACI GRCGYC 2 cut(s) 38, 170
BstC8I GCNNGC 2 cut(s) 168, 224
BstDEI CTNAG 4 cut(s) 85, 267, 452, 525
BstDSI CCRYGG 3 cut(s) 33, 838, 857
BstF5I GGATG 2 cut(s) 34, 406
BstFNI CGCG 2 cut(s) 69, 260
BstH2I RGCGCY 1 cut(s) 173
BstHHI GCGC 3 cut(s) 172, 262, 689
BstKTI GATC 3 cut(s) 196, 451, 580
BstMAI GTCTC 2 cut(s) 719, 899
BstMBI GATC 3 cut(s) 193, 448, 577
BstMWI GCNNNNNNNGC 7 cut(s) 57, 66, 178, 268, 695, 707, 716
BstSCI CCNGG 1 cut(s) 24
BstSFI CTRYAG 2 cut(s) 638, 889
BstUI CGCG 2 cut(s) 69, 260
BstV1I GCAGC 6 cut(s) 92, 190, 629, 685, 955, 958
BstV2I GAAGAC 5 cut(s) 12, 213, 369, 441, 552
BstX2I RGATCY 1 cut(s) 193
BstYI RGATCY 1 cut(s) 193
Bsu15I ATCGAT 1 cut(s) 580
BsuI GTATCC 1 cut(s) 617
BsuTUI ATCGAT 1 cut(s) 580
BtgI CCRYGG 3 cut(s) 33, 838, 857
BtgZI GCGATG 1 cut(s) 147
BtsCI GGATG 2 cut(s) 34, 406
BtsI GCAGTG 1 cut(s) 55
BtsIMutI CAGTG 2 cut(s) 43, 55
Cac8I GCNNGC 2 cut(s) 168, 224
CaiI CAGNNNCTG 1 cut(s) 965
CfoI GCGC 3 cut(s) 172, 262, 689
Cfr10I RCCGGY 2 cut(s) 166, 694
ClaI ATCGAT 1 cut(s) 580
CseI GACGC 3 cut(s) 27, 58, 493
CspCI CAANNNNNGTGG 2 cut(s) 827, 862
CviAII CATG 1 cut(s) 858
DdeI CTNAG 4 cut(s) 85, 267, 452, 525
DinI GGCGCC 1 cut(s) 171
DpnI GATC 3 cut(s) 195, 450, 579
DpnII GATC 3 cut(s) 193, 448, 577
DrdI GACNNNNNNGTC 1 cut(s) 487
DseDI GACNNNNNNGTC 1 cut(s) 487
Eam1104I CTCTTC 3 cut(s) 390, 402, 924
EarI CTCTTC 3 cut(s) 390, 402, 924
EciI GGCGGA 1 cut(s) 687
Ecl136II GAGCTC 1 cut(s) 331
Eco130I CCWWGG 1 cut(s) 857
Eco24I GRGCYC 1 cut(s) 333
Eco53kI GAGCTC 1 cut(s) 331
Eco57I CTGAAG 1 cut(s) 304
EcoICRI GAGCTC 1 cut(s) 331
EcoRI GAATTC 1 cut(s) 510
EcoT14I CCWWGG 1 cut(s) 857
EcoT38I GRGCYC 1 cut(s) 333
EgeI GGCGCC 1 cut(s) 171
EheI GGCGCC 1 cut(s) 171
ErhI CCWWGG 1 cut(s) 857
FaeI CATG 1 cut(s) 861
FaiI YATR 5 cut(s) 569, 608, 764, 859, 924
FalI AAGNNNNNCTT 2 cut(s) 862, 894
FatI CATG 1 cut(s) 857
FauI CCCGC 1 cut(s) 157
Fnu4HI GCNGC 7 cut(s) 106, 153, 179, 618, 699, 969, 972
FokI GGATG 2 cut(s) 41, 413
FriOI GRGCYC 1 cut(s) 333
Fsp4HI GCNGC 7 cut(s) 106, 153, 179, 618, 699, 969, 972
GlaI GCGC 3 cut(s) 171, 261, 688
GluI GCNGC 7 cut(s) 106, 153, 179, 618, 699, 969, 972
GsuI CTGGAG 2 cut(s) 196, 218
HaeII RGCGCY 1 cut(s) 173
HapII CCGG 6 cut(s) 26, 167, 498, 596, 621, 695
HgaI GACGC 3 cut(s) 27, 58, 493
HhaI GCGC 3 cut(s) 172, 262, 689
Hin1I GRCGYC 2 cut(s) 38, 170
Hin1II CATG 1 cut(s) 861
Hin6I GCGC 3 cut(s) 170, 260, 687
HinP1I GCGC 3 cut(s) 170, 260, 687
HincII GTYRAC 1 cut(s) 424
HindII GTYRAC 1 cut(s) 424
HindIII AAGCTT 1 cut(s) 630
HinfI GANTC 7 cut(s) 131, 145, 209, 540, 572, 894, 962
HpaII CCGG 6 cut(s) 26, 167, 498, 596, 621, 695
HphI GGTGA 2 cut(s) 267, 805
Hpy166II GTNNAC 3 cut(s) 275, 424, 827
Hpy188I TCNGA 6 cut(s) 7, 198, 453, 577, 748, 961
Hpy188III TCNNGA 4 cut(s) 235, 478, 492, 515
Hpy8I GTNNAC 3 cut(s) 275, 424, 827
Hpy99I CGWCG 9 cut(s) 19, 43, 74, 77, 131, 356, 431, 484, 487
HpyAV CCTTC 3 cut(s) 273, 647, 672
HpyCH4III ACNGT 3 cut(s) 421, 822, 955
HpyCH4V TGCA 2 cut(s) 60, 617
HpyF10VI GCNNNNNNNGC 7 cut(s) 57, 66, 178, 268, 695, 707, 716
HpyF3I CTNAG 4 cut(s) 85, 267, 452, 525
Hsp92I GRCGYC 2 cut(s) 38, 170
Hsp92II CATG 1 cut(s) 861
HspAI GCGC 3 cut(s) 170, 260, 687
KasI GGCGCC 1 cut(s) 169
KroI GCCGGC 1 cut(s) 166
KroNI GCCGGC 1 cut(s) 168
Kzo9I GATC 3 cut(s) 193, 448, 577
LmnI GCTCC 3 cut(s) 16, 246, 328
Lsp1109I GCAGC 6 cut(s) 92, 190, 629, 685, 955, 958
LweI GCATC 4 cut(s) 19, 228, 604, 904
MaeIII GTNAC 4 cut(s) 113, 816, 833, 998
MalI GATC 3 cut(s) 195, 450, 579
MboI GATC 3 cut(s) 193, 448, 577
MflI RGATCY 1 cut(s) 193
MhlI GDGCHC 1 cut(s) 333
MluCI AATT 5 cut(s) 135, 412, 510, 550, 643
MluI ACGCGT 1 cut(s) 67
Mly113I GGCGCC 1 cut(s) 170
MlyI GAGTC 3 cut(s) 139, 140, 203
MmeI TCCRAC 1 cut(s) 306
MreI CGCCGGCG 1 cut(s) 166
MroNI GCCGGC 1 cut(s) 166
MseI TTAA 1 cut(s) 416
MslI CAYNNNNRTG 1 cut(s) 291
MspI CCGG 6 cut(s) 26, 167, 498, 596, 621, 695
MspR9I CCNGG 1 cut(s) 26
MvnI CGCG 2 cut(s) 69, 260
MwoI GCNNNNNNNGC 7 cut(s) 57, 66, 178, 268, 695, 707, 716
NaeI GCCGGC 1 cut(s) 168
NarI GGCGCC 1 cut(s) 170
NciI CCSGG 1 cut(s) 26
NcoI CCATGG 1 cut(s) 857
NdeII GATC 3 cut(s) 193, 448, 577
NgoMIV GCCGGC 1 cut(s) 166
NlaIII CATG 1 cut(s) 861
NlaIV GGNNCC 1 cut(s) 171
PcsI WCGNNNNNNNCGW 5 cut(s) 105, 351, 357, 435, 488
PdiI GCCGGC 1 cut(s) 168
PfeI GAWTC 4 cut(s) 540, 572, 894, 962
PflFI GACNNNGTC 2 cut(s) 95, 487
PkrI GCNGC 7 cut(s) 107, 154, 180, 619, 700, 970, 973
PleI GAGTC 3 cut(s) 139, 139, 203
PluTI GGCGCC 1 cut(s) 173
PpsI GAGTC 3 cut(s) 139, 139, 203
Psp124BI GAGCTC 1 cut(s) 333
PspN4I GGNNCC 1 cut(s) 171
PstNI CAGNNNCTG 1 cut(s) 965
PsuI RGATCY 1 cut(s) 193
PsyI GACNNNGTC 2 cut(s) 95, 487
RseI CAYNNNNRTG 1 cut(s) 291
SacI GAGCTC 1 cut(s) 333
SaqAI TTAA 1 cut(s) 416
SatI GCNGC 7 cut(s) 106, 153, 179, 618, 699, 969, 972
Sau3AI GATC 3 cut(s) 193, 448, 577
SchI GAGTC 3 cut(s) 139, 140, 203
ScrFI CCNGG 1 cut(s) 26
SduI GDGCHC 1 cut(s) 333
SetI ASST 8 cut(s) 86, 124, 291, 326, 333, 634, 738, 903
SfaNI GCATC 4 cut(s) 19, 228, 604, 904
SfcI CTRYAG 2 cut(s) 638, 889
SfoI GGCGCC 1 cut(s) 171
SgrAI CRCCGGYG 1 cut(s) 166
SmiMI CAYNNNNRTG 1 cut(s) 291
SmlI CTYRAG 1 cut(s) 902
SmoI CTYRAG 1 cut(s) 902
Sse9I AATT 5 cut(s) 135, 412, 510, 550, 643
SsiI CCGC 5 cut(s) 150, 153, 176, 244, 672
SspDI GGCGCC 1 cut(s) 169
SstI GAGCTC 1 cut(s) 333
StyD4I CCNGG 1 cut(s) 24
StyI CCWWGG 1 cut(s) 857
TaaI ACNGT 3 cut(s) 421, 822, 955
TaqI TCGA 8 cut(s) 129, 351, 491, 516, 543, 554, 580, 732
TasI AATT 5 cut(s) 135, 412, 510, 550, 643
TauI GCSGC 1 cut(s) 155
TfiI GAWTC 4 cut(s) 540, 572, 894, 962
Tru1I TTAA 1 cut(s) 416
Tru9I TTAA 1 cut(s) 416
TscAI CASTG 2 cut(s) 50, 62
TseI GCWGC 6 cut(s) 105, 178, 617, 698, 968, 971
TspDTI ATGAA 3 cut(s) 150, 387, 402
TspGWI ACGGA 2 cut(s) 554, 855
TspRI CASTG 2 cut(s) 50, 62
Tth111I GACNNNGTC 2 cut(s) 95, 487
XapI RAATTY 3 cut(s) 412, 510, 643
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.