Rroxscaffold_7G00165020

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0014025
Physical Location & Seq
Reverse (-)
6917252 .. 6918755
1504 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00165020.1

Sequence Viewer

Length: 1113 bp
ATGGCCGAAACTCCATCTCAGTCGCCGCCGCCGCGATCAGTCTCCGACACGGAGACCGAGACGGAGGCGCAGCCGCAGGAGTACTGGTGCTACCACTGCAACAAGCGCGTCTCTGTCGAGACCGTCGCCAACCTCCCGGACATCGTCTGCCACGACTGCAAGAACGGGTTCGTGGAATCGATTCTACCGTCGGATCATCCTCTTTCCCGGCCGTCCGATCAGGCCGACGACCCGACCTTCGGCTCCCCGTTCCTCCAGGTGCTCCGGCTGATCGCGCAGGCGGCGCGTGAGGAGGACTCGCCACCGCCGCTGCCTCAGAACCCCACCCCCGAGGAGGATTTCCTCAGGATTGAGCTCGACGGCTGGGACAACGACGAGGACGAGGACGACGCGCACAGCGTCGAGTTTCACAATGAAGGCGGCGAGGAAAACGAGGAGGCGGAAGAGGAGGAGGAGGAGGAGGAGGAGGAGGAAGAAGAAGAGGAGGATAGATCTGATCGCGAGGAGGAAGAGAATCAAGGAGATCGGCAAGAGCGAGACGAAGAAGACCTGCGACGACGTCGTCGCGATGTGCTGCGTCTCCGCATCAGAGACATTGCTACCCGGGCCCGGAGCGGGAGGAACCGGATTCTCGATTGGGCCGAGATCTTGATGGGCCTGGAGGACAACTCCATCGAATTGCGGTTCGAGATGCCCGAATCGGATCGTTACGTAGGCAATCCTGAGGATTACGTGGACGCAGCTGGGTATGAAGCTCTGCTTCAGACCTTGGCGGAGAGCGATAGCAGTGGAAGGAGAGGAGCCCCACCGGCTTCGAAAGCAGCTGTATTGGAATTGCCAGCGGTGGAGATTAAGTCTGTGGATGAGGCTGTGGTTTGTGCCATATGTAAGGATATGGTTAATGTTGGTGAAATGTCAAAGAAGCTGCCATGCGGGCATGGATATCATGGTGACTGCATTGTGCCATGGCTGAGCTCTAGGAATTCCTGCCCGGTTTGCCGGTTTGAGCTGCCCACCGATGATCCTGAATACGAGGAGGAGAGGAAGAAGCAAGCTGTCAATGTGAGTGCCGGAGCTTCGGGTTCTGGTGGAGATAATTCAGTCTCCAGCTGA

Protein Analysis

370

Amino Acids

41.59

Weight (kDa)

4.28

Isoelectric Point (pI)

85.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zn_ribbon_19 PF14369 27 - 61 3.7e-10 zinc-ribbon
zf-RING_2 PF13639 293 - 334 3.7e-12 Ring finger domain
zf-C3HC4_2 PF13923 293 - 333 9.1e-11 Zinc finger, C3HC4 type (RING finger)
zf-RING_11 PF17123 293 - 320 1.1e-07 RING-like zinc finger
zf-C3HC4 PF00097 293 - 333 1.6e-07 Zinc finger, C3HC4 type (RING finger)
zf-rbx1 PF12678 310 - 334 1.1e-06 RING-H2 zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014025)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G64920
fragaria_vesca FvH4_2g38280
malus_domestica MD08G1011000.v1.1 MD15G1011000.v1.1
prunus_persica Prupe.1G363700_v2.0.a1
pyrus_communis pycom15g00940
rosa_chinensis RchiOBHm_Chr6g0303101
rosa_laevigata RLG00000011108
rosa_multiflora Rmu_sc0003721.1_g000001 Rmu_sc0008911.1_g000004
rosa_roxburghii Rroxscaffold_7G00165020
rosa_rugosa Rorug06G0319200
rosa_samantha Rh6AG433000 Rh6BG469700 Rh6CG445300 Rh6DG432100
rosa_wichuraiana Rw6G037530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 561
AatII GACGTC 1 cut(s) 562
Acc36I ACCTGC 1 cut(s) 558
AccBSI CCGCTC 1 cut(s) 615
AccII CGCG 7 cut(s) 34, 108, 275, 286, 392, 501, 567
AclWI GGATC 3 cut(s) 201, 711, 1016
AcoI YGGCCR 2 cut(s) 3, 209
AcsI RAATTY 1 cut(s) 982
AcuI CTGAAG 1 cut(s) 746
AcyI GRCGYC 1 cut(s) 559
AfaI GTAC 1 cut(s) 83
AfiI CCNNNNNNNGG 4 cut(s) 239, 334, 609, 615
AjnI CCWGG 2 cut(s) 255, 657
Alw21I GWGCWC 3 cut(s) 264, 357, 977
Alw26I GTCTC 9 cut(s) 46, 47, 53, 113, 115, 531, 584, 585, 1108
AlwI GGATC 3 cut(s) 201, 711, 1016
Ama87I CYCGRG 2 cut(s) 329, 603
AoxI GGCC 6 cut(s) 3, 209, 222, 606, 639, 655
ApaI GGGCCC 1 cut(s) 610
ApeKI GCWGC 7 cut(s) 70, 310, 574, 740, 821, 925, 1009
ApoI RAATTY 1 cut(s) 982
ArsI GACNNNNNNTTYG 2 cut(s) 221, 253
Asp700I GAANNNNTTC 2 cut(s) 167, 180
AspLEI GCGC 5 cut(s) 70, 108, 277, 286, 394
AspS9I GGNCC 4 cut(s) 606, 607, 639, 655
AsuC2I CCSGG 6 cut(s) 137, 208, 604, 605, 610, 992
AsuHPI GGTGA 2 cut(s) 920, 962
AsuII TTCGAA 1 cut(s) 815
AvaI CYCGRG 2 cut(s) 329, 603
AxyI CCTNAGG 2 cut(s) 344, 723
BaeGI GKGCMC 1 cut(s) 610
BanII GRGCYC 4 cut(s) 357, 610, 805, 977
BbsI GAAGAC 1 cut(s) 552
Bbv12I GWGCWC 3 cut(s) 264, 357, 977
BbvI GCAGC 7 cut(s) 82, 297, 561, 752, 833, 912, 996
BccI CCATC 3 cut(s) 22, 646, 680
BceAI ACGGC 2 cut(s) 196, 376
BciT130I CCWGG 2 cut(s) 257, 659
BcnI CCSGG 6 cut(s) 137, 208, 604, 605, 610, 992
BcoDI GTCTC 9 cut(s) 46, 47, 53, 113, 115, 531, 584, 585, 1108
BfaI CTAG 1 cut(s) 978
BfuAI ACCTGC 1 cut(s) 558
BglI GCCNNNNNGGC 2 cut(s) 809, 934
BglII AGATCT 2 cut(s) 491, 645
BlpI GCTNAGC 1 cut(s) 971
BmcAI AGTACT 1 cut(s) 83
Bme1390I CCNGG 8 cut(s) 137, 208, 257, 604, 605, 610, 659, 992
BmeT110I CYCGRG 2 cut(s) 329, 603
BmgT120I GGNCC 4 cut(s) 606, 607, 639, 655
BmiI GGNNCC 4 cut(s) 244, 608, 623, 802
BmrFI CCNGG 8 cut(s) 137, 208, 257, 604, 605, 610, 659, 992
BmsI GCATC 2 cut(s) 594, 681
BpiI GAAGAC 1 cut(s) 552
BplI GAGNNNNNCTC 4 cut(s) 281, 313, 653, 685
BpmI CTGGAG 3 cut(s) 239, 680, 1090
Bpu1102I GCTNAGC 1 cut(s) 971
Bpu14I TTCGAA 1 cut(s) 815
BpuMI CCSGG 6 cut(s) 137, 208, 604, 605, 610, 992
Bsa29I ATCGAT 1 cut(s) 179
BsaAI YACGTR 2 cut(s) 712, 733
BsaHI GRCGYC 1 cut(s) 559
BsaI GGTCTC 2 cut(s) 47, 113
BsaJI CCNNGG 4 cut(s) 330, 603, 768, 965
BsaWI WCCGGW 1 cut(s) 624
BsaXI ACNNNNNCTCC 4 cut(s) 71, 101, 839, 869
Bsc4I CCNNNNNNNGG 4 cut(s) 239, 334, 609, 615
Bse118I RCCGGY 2 cut(s) 808, 999
Bse1I ACTGG 1 cut(s) 89
Bse21I CCTNAGG 2 cut(s) 344, 723
Bse3DI GCAATG 1 cut(s) 594
BseBI CCWGG 2 cut(s) 257, 659
BseCI ATCGAT 1 cut(s) 179
BseDI CCNNGG 4 cut(s) 330, 603, 768, 965
BseGI GGATG 2 cut(s) 196, 868
BseLI CCNNNNNNNGG 4 cut(s) 239, 334, 609, 615
BseMI GCAATG 1 cut(s) 594
BseMII CTCAG 5 cut(s) 32, 329, 358, 714, 962
BseNI ACTGG 1 cut(s) 89
BseSI GKGCMC 1 cut(s) 610
BseX3I CGGCCG 1 cut(s) 209
BseXI GCAGC 7 cut(s) 82, 297, 561, 752, 833, 912, 996
BseYI CCCAGC 2 cut(s) 363, 743
Bsh1236I CGCG 7 cut(s) 34, 108, 275, 286, 392, 501, 567
Bsh1285I CGRYCG 1 cut(s) 212
BshFI GGCC 6 cut(s) 5, 211, 224, 608, 641, 657
BshVI ATCGAT 1 cut(s) 179
BsiEI CGRYCG 1 cut(s) 212
BsiHKAI GWGCWC 3 cut(s) 264, 357, 977
BsiHKCI CYCGRG 2 cut(s) 329, 603
BslFI GGGAC 1 cut(s) 380
BslI CCNNNNNNNGG 4 cut(s) 239, 334, 609, 615
BsmAI GTCTC 9 cut(s) 46, 47, 53, 113, 115, 531, 584, 585, 1108
BsmBI CGTCTC 4 cut(s) 53, 115, 531, 584
BsmFI GGGAC 1 cut(s) 380
BsnI GGCC 6 cut(s) 5, 211, 224, 608, 641, 657
Bso31I GGTCTC 2 cut(s) 47, 113
BsoBI CYCGRG 2 cut(s) 329, 603
Bsp119I TTCGAA 1 cut(s) 815
Bsp120I GGGCCC 1 cut(s) 606
Bsp1286I GDGCHC 5 cut(s) 264, 357, 610, 805, 977
Bsp1720I GCTNAGC 1 cut(s) 971
Bsp19I CCATGG 1 cut(s) 965
Bsp68I TCGCGA 2 cut(s) 501, 567
BspANI GGCC 6 cut(s) 5, 211, 224, 608, 641, 657
BspCNI CTCAG 5 cut(s) 31, 328, 357, 715, 963
BspDI ATCGAT 1 cut(s) 179
BspFNI CGCG 7 cut(s) 34, 108, 275, 286, 392, 501, 567
BspLI GGNNCC 4 cut(s) 244, 608, 623, 802
BspMI ACCTGC 1 cut(s) 558
BspPI GGATC 3 cut(s) 201, 711, 1016
BspT104I TTCGAA 1 cut(s) 815
BspTNI GGTCTC 2 cut(s) 47, 113
BsrBI CCGCTC 1 cut(s) 615
BsrDI GCAATG 1 cut(s) 594
BsrFI RCCGGY 2 cut(s) 808, 999
BsrI ACTGG 1 cut(s) 89
BssAI RCCGGY 2 cut(s) 808, 999
BssECI CCNNGG 4 cut(s) 330, 603, 768, 965
BssNI GRCGYC 1 cut(s) 559
BssT1I CCWWGG 2 cut(s) 768, 965
Bst2UI CCWGG 2 cut(s) 257, 659
Bst4CI ACNGT 2 cut(s) 124, 189
Bst6I CTCTTC 3 cut(s) 438, 474, 504
BstACI GRCGYC 1 cut(s) 559
BstBAI YACGTR 2 cut(s) 712, 733
BstBI TTCGAA 1 cut(s) 815
BstC8I GCNNGC 4 cut(s) 279, 840, 935, 1053
BstDEI CTNAG 5 cut(s) 18, 315, 344, 723, 971
BstDSI CCRYGG 1 cut(s) 965
BstF5I GGATG 2 cut(s) 196, 868
BstFNI CGCG 7 cut(s) 34, 108, 275, 286, 392, 501, 567
BstHHI GCGC 5 cut(s) 70, 108, 277, 286, 394
BstMAI GTCTC 9 cut(s) 46, 47, 53, 113, 115, 531, 584, 585, 1108
BstMCI CGRYCG 1 cut(s) 212
BstNI CCWGG 2 cut(s) 257, 659
BstSCI CCNGG 8 cut(s) 135, 206, 255, 602, 603, 608, 657, 990
BstSLI GKGCMC 1 cut(s) 610
BstSNI TACGTA 1 cut(s) 712
BstUI CGCG 7 cut(s) 34, 108, 275, 286, 392, 501, 567
BstV1I GCAGC 7 cut(s) 82, 297, 561, 752, 833, 912, 996
BstV2I GAAGAC 1 cut(s) 552
BstX2I RGATCY 2 cut(s) 491, 645
BstYI RGATCY 2 cut(s) 491, 645
BstZI CGGCCG 1 cut(s) 209
Bsu15I ATCGAT 1 cut(s) 179
Bsu36I CCTNAGG 2 cut(s) 344, 723
BsuRI GGCC 6 cut(s) 5, 211, 224, 608, 641, 657
BsuTUI ATCGAT 1 cut(s) 179
BtgI CCRYGG 1 cut(s) 965
BtgZI GCGATG 1 cut(s) 582
BtsCI GGATG 2 cut(s) 196, 868
BtsI GCAGTG 2 cut(s) 94, 793
BtsIMutI CAGTG 2 cut(s) 94, 793
BtuMI TCGCGA 2 cut(s) 501, 567
BveI ACCTGC 1 cut(s) 558
Cac8I GCNNGC 4 cut(s) 279, 840, 935, 1053
CfoI GCGC 5 cut(s) 70, 108, 277, 286, 394
Cfr10I RCCGGY 2 cut(s) 808, 999
Cfr13I GGNCC 4 cut(s) 606, 607, 639, 655
Cfr9I CCCGGG 1 cut(s) 603
ClaI ATCGAT 1 cut(s) 179
CseI GACGC 5 cut(s) 97, 388, 398, 566, 746
Csp6I GTAC 1 cut(s) 82
CviAII CATG 4 cut(s) 930, 938, 947, 966
CviQI GTAC 1 cut(s) 82
DdeI CTNAG 5 cut(s) 18, 315, 344, 723, 971
DrdI GACNNNNNNGTC 1 cut(s) 561
DseDI GACNNNNNNGTC 1 cut(s) 561
EaeI YGGCCR 2 cut(s) 3, 209
EagI CGGCCG 1 cut(s) 209
Eam1104I CTCTTC 3 cut(s) 438, 474, 504
EarI CTCTTC 3 cut(s) 438, 474, 504
EciI GGCGGA 2 cut(s) 455, 788
Ecl136II GAGCTC 2 cut(s) 355, 975
EclXI CGGCCG 1 cut(s) 209
Eco105I TACGTA 1 cut(s) 712
Eco130I CCWWGG 2 cut(s) 768, 965
Eco24I GRGCYC 4 cut(s) 357, 610, 805, 977
Eco31I GGTCTC 2 cut(s) 47, 113
Eco32I GATATC 1 cut(s) 944
Eco52I CGGCCG 1 cut(s) 209
Eco53kI GAGCTC 2 cut(s) 355, 975
Eco57I CTGAAG 1 cut(s) 746
Eco81I CCTNAGG 2 cut(s) 344, 723
Eco88I CYCGRG 2 cut(s) 329, 603
EcoICRI GAGCTC 2 cut(s) 355, 975
EcoRI GAATTC 1 cut(s) 982
EcoRII CCWGG 2 cut(s) 255, 657
EcoRV GATATC 1 cut(s) 944
EcoT14I CCWWGG 2 cut(s) 768, 965
EcoT38I GRGCYC 4 cut(s) 357, 610, 805, 977
ErhI CCWWGG 2 cut(s) 768, 965
Esp3I CGTCTC 4 cut(s) 53, 115, 531, 584
FaeI CATG 4 cut(s) 933, 941, 950, 969
FaiI YATR 8 cut(s) 750, 884, 886, 896, 931, 939, 948, 967
FalI AAGNNNNNCTT 2 cut(s) 744, 776
FaqI GGGAC 1 cut(s) 380
FatI CATG 4 cut(s) 929, 937, 946, 965
FauI CCCGC 2 cut(s) 608, 926
FauNDI CATATG 1 cut(s) 884
FokI GGATG 2 cut(s) 183, 875
FriOI GRGCYC 4 cut(s) 357, 610, 805, 977
FspBI CTAG 1 cut(s) 978
GlaI GCGC 5 cut(s) 69, 107, 276, 285, 393
GsaI CCCAGC 2 cut(s) 367, 747
GsuI CTGGAG 3 cut(s) 239, 680, 1090
HaeIII GGCC 6 cut(s) 5, 211, 224, 608, 641, 657
HgaI GACGC 5 cut(s) 97, 388, 398, 566, 746
HhaI GCGC 5 cut(s) 70, 108, 277, 286, 394
Hin1I GRCGYC 1 cut(s) 559
Hin1II CATG 4 cut(s) 933, 941, 950, 969
Hin6I GCGC 5 cut(s) 68, 106, 275, 284, 392
HinP1I GCGC 5 cut(s) 68, 106, 275, 284, 392
HinfI GANTC 6 cut(s) 176, 181, 296, 514, 628, 698
HphI GGTGA 2 cut(s) 920, 962
Hpy166II GTNNAC 1 cut(s) 736
Hpy188I TCNGA 8 cut(s) 46, 193, 217, 318, 496, 590, 703, 765
Hpy188III TCNNGA 9 cut(s) 118, 346, 500, 566, 632, 649, 688, 722, 1025
Hpy8I GTNNAC 1 cut(s) 736
HpyAV CCTTC 3 cut(s) 247, 410, 786
HpyCH4III ACNGT 2 cut(s) 124, 189
HpyCH4IV ACGT 3 cut(s) 559, 711, 732
HpyCH4V TGCA 3 cut(s) 99, 159, 957
HpyF3I CTNAG 5 cut(s) 18, 315, 344, 723, 971
HpySE526I ACGT 3 cut(s) 559, 711, 732
Hsp92I GRCGYC 1 cut(s) 559
Hsp92II CATG 4 cut(s) 933, 941, 950, 969
HspAI GCGC 5 cut(s) 68, 106, 275, 284, 392
LmnI GCTCC 5 cut(s) 248, 267, 612, 800, 1073
Lsp1109I GCAGC 7 cut(s) 82, 297, 561, 752, 833, 912, 996
LweI GCATC 2 cut(s) 594, 681
MaeI CTAG 1 cut(s) 978
MaeII ACGT 3 cut(s) 559, 711, 732
MaeIII GTNAC 2 cut(s) 707, 950
MbiI CCGCTC 1 cut(s) 615
MboII GAAGA 8 cut(s) 455, 485, 488, 491, 521, 554, 557, 1057
MflI RGATCY 2 cut(s) 491, 645
MhlI GDGCHC 5 cut(s) 264, 357, 610, 805, 977
MluCI AATT 4 cut(s) 677, 833, 982, 1096
MlyI GAGTC 1 cut(s) 290
MmeI TCCRAC 2 cut(s) 69, 171
MroXI GAANNNNTTC 2 cut(s) 167, 180
MseI TTAA 2 cut(s) 852, 900
MspA1I CMGCKG 5 cut(s) 310, 743, 824, 842, 1110
MspR9I CCNGG 8 cut(s) 137, 208, 257, 604, 605, 610, 659, 992
MvaI CCWGG 2 cut(s) 257, 659
MvnI CGCG 7 cut(s) 34, 108, 275, 286, 392, 501, 567
NciI CCSGG 6 cut(s) 137, 208, 604, 605, 610, 992
NcoI CCATGG 1 cut(s) 965
NdeI CATATG 1 cut(s) 884
NlaIII CATG 4 cut(s) 933, 941, 950, 969
NlaIV GGNNCC 4 cut(s) 244, 608, 623, 802
NmeAIII GCCGAG 1 cut(s) 667
NmuCI GTSAC 1 cut(s) 950
NruI TCGCGA 2 cut(s) 501, 567
NspV TTCGAA 1 cut(s) 815
PcsI WCGNNNNNNNCGW 7 cut(s) 150, 185, 378, 396, 532, 639, 693
PdmI GAANNNNTTC 2 cut(s) 167, 180
PfeI GAWTC 5 cut(s) 176, 181, 514, 628, 698
PflFI GACNNNGTC 3 cut(s) 143, 558, 561
PfoI TCCNGGA 1 cut(s) 135
PleI GAGTC 1 cut(s) 290
PpsI GAGTC 1 cut(s) 290
Ppu21I YACGTR 2 cut(s) 712, 733
Psp124BI GAGCTC 2 cut(s) 357, 977
Psp6I CCWGG 2 cut(s) 255, 657
PspFI CCCAGC 2 cut(s) 363, 743
PspGI CCWGG 2 cut(s) 255, 657
PspN4I GGNNCC 4 cut(s) 244, 608, 623, 802
PspOMI GGGCCC 1 cut(s) 606
PspPI GGNCC 4 cut(s) 606, 607, 639, 655
PsuI RGATCY 2 cut(s) 491, 645
PsyI GACNNNGTC 3 cut(s) 143, 558, 561
PvuII CAGCTG 3 cut(s) 743, 824, 1110
RruI TCGCGA 2 cut(s) 501, 567
RsaI GTAC 1 cut(s) 83
RsaNI GTAC 1 cut(s) 82
SacI GAGCTC 2 cut(s) 357, 977
SaqAI TTAA 2 cut(s) 852, 900
Sau96I GGNCC 4 cut(s) 606, 607, 639, 655
ScaI AGTACT 1 cut(s) 83
SchI GAGTC 1 cut(s) 290
ScrFI CCNGG 8 cut(s) 137, 208, 257, 604, 605, 610, 659, 992
SduI GDGCHC 5 cut(s) 264, 357, 610, 805, 977
SfaNI GCATC 2 cut(s) 594, 681
SfuI TTCGAA 1 cut(s) 815
SmaI CCCGGG 1 cut(s) 605
SnaBI TACGTA 1 cut(s) 712
Sse9I AATT 4 cut(s) 677, 833, 982, 1096
SspMI CTAG 1 cut(s) 978
SstI GAGCTC 2 cut(s) 357, 977
StyD4I CCNGG 8 cut(s) 135, 206, 255, 602, 603, 608, 657, 990
StyI CCWWGG 2 cut(s) 768, 965
TaaI ACNGT 2 cut(s) 124, 189
TaiI ACGT 3 cut(s) 562, 714, 735
TaqI TCGA 8 cut(s) 117, 179, 357, 402, 633, 675, 687, 815
TaqII GACCGA 1 cut(s) 71
TasI AATT 4 cut(s) 677, 833, 982, 1096
TatI WGTACW 1 cut(s) 81
TauI GCSGC 7 cut(s) 28, 31, 34, 76, 284, 310, 423
TfiI GAWTC 5 cut(s) 176, 181, 514, 628, 698
Tru1I TTAA 2 cut(s) 852, 900
Tru9I TTAA 2 cut(s) 852, 900
TscAI CASTG 2 cut(s) 101, 793
TseFI GTSAC 1 cut(s) 950
TseI GCWGC 7 cut(s) 70, 310, 574, 740, 821, 925, 1009
Tsp45I GTSAC 1 cut(s) 950
TspDTI ATGAA 2 cut(s) 429, 765
TspGWI ACGGA 2 cut(s) 65, 77
TspMI CCCGGG 1 cut(s) 603
TspRI CASTG 2 cut(s) 101, 793
Tth111I GACNNNGTC 3 cut(s) 143, 558, 561
XapI RAATTY 1 cut(s) 982
XmaI CCCGGG 1 cut(s) 603
XmnI GAANNNNTTC 2 cut(s) 167, 180
XspI CTAG 1 cut(s) 978
ZraI GACGTC 1 cut(s) 560
ZrmI AGTACT 1 cut(s) 83
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.