Rh6AG433000

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0014025
Physical Location & Seq
Forward (+)
62309748 .. 62311606
1859 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG433000.1

Sequence Viewer

Length: 1104 bp
ATGGCCGAAACTCCATCTCAGTCGCCGCCGCCGCGATCCGTCTCCGACACGGAGACCGAGACGGAGGCGCAGCCGCAGGAGTTCTGGTGCTACCACTGCAACAAGCGCGTCTCCGTCGAGACGGTCGCCAACCTCCCGGACATCGTCTGCCACGACTGCAAGAACGGGTTCGTGGAATCGATTCCACCGTCGGATCATCCTCTTTCCCGGCCGTCCGATCAGGCGGACGACCCGACCTTCGGCTCCCCGTTCCTCCAGGTGCTCCGTCTGATCGCGCAGGCGGCGCGTGAGGAGGACACGCCCCCGCCGCTGCCTCAGAACCCCACCCCCGAGGAAGATTTCCTCAGGATTGAGCTCGACGGCTGGGACAACGACGAGGACGACGAGGACGCGCACAGCGTCGAGTTTCACAATGAAGGCGGCGAGGAAAACGAGGAGGCGGAAGAGGAGGAGGAGGAGGAGGAGGAAGAAGAAGAGGATAGATCTGATCGCGAGGAGGAAGAGAATCAAGGAGATCGGCAAGAGCGAGACGAAGAAGACCTGCGACGACGTCGTCGCGATGTGCTGCGTCTCCGCATCAGAGACATTGCTACCCGGGCCCGGAGCGGGAGGAACCGGATTCTGGATTGGGCCGAGATCTTGATGGGCCTGGAGGACAACTCCATCGAATTACGGTTCGAGATGCCCGAATCGGATCGTTACGTAGGCAATCCTGAGGATTACGTTGACGCAGCTGGGTATGAAGCTCTGCTTCAGACCTTGGCGGAGACCGATAGCAGTGGAAGGAGAGGAGCTCCACCGGCTTCGAAAGCAGCCGTATCGGAATTGCCAGCGGTGGAGATTAAGTCTGTGGATGAGGCTGTGGTTTGTGCCATATGCAAGGATATGGTTAATGTTGGTGAAATGTCAAAGAAGCTGCCATGCGGGCATGGATATCATGGTGACTGCATTGTGCCATGGCTGAGCTCTAGGAATTCCTGCCCGGTTTGCCGGTTTGAGCTGCCCACCGATGATCCTGAATACGAGGAGGAGAGGAAGAAGCAAGCTGTCAATGTGAGTGCCGGAGCTTCGGGTTCTGGTGGAGATAATTCAGTCTCCGGCTGA

Protein Analysis

367

Amino Acids

41.15

Weight (kDa)

4.3

Isoelectric Point (pI)

80.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zn_ribbon_19 PF14369 27 - 61 1.4e-09 zinc-ribbon
zf-RING_2 PF13639 290 - 331 3.6e-12 Ring finger domain
zf-C3HC4_2 PF13923 290 - 330 9e-11 Zinc finger, C3HC4 type (RING finger)
zf-RING_11 PF17123 290 - 317 1e-07 RING-like zinc finger
zf-C3HC4 PF00097 290 - 330 1.6e-07 Zinc finger, C3HC4 type (RING finger)
zf-rbx1 PF12678 307 - 331 1.1e-06 RING-H2 zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014025)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G64920
fragaria_vesca FvH4_2g38280
malus_domestica MD08G1011000.v1.1 MD15G1011000.v1.1
prunus_persica Prupe.1G363700_v2.0.a1
pyrus_communis pycom15g00940
rosa_chinensis RchiOBHm_Chr6g0303101
rosa_laevigata RLG00000011108
rosa_multiflora Rmu_sc0003721.1_g000001 Rmu_sc0008911.1_g000004
rosa_roxburghii Rroxscaffold_7G00165020
rosa_rugosa Rorug06G0319200
rosa_samantha Rh6AG433000 Rh6BG469700 Rh6CG445300 Rh6DG432100
rosa_wichuraiana Rw6G037530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 552
AatII GACGTC 1 cut(s) 553
Acc36I ACCTGC 1 cut(s) 549
AccBSI CCGCTC 1 cut(s) 606
AccII CGCG 7 cut(s) 34, 108, 275, 286, 392, 492, 558
AclWI GGATC 4 cut(s) 30, 201, 702, 1007
AcoI YGGCCR 2 cut(s) 3, 209
AcsI RAATTY 1 cut(s) 973
AcuI CTGAAG 1 cut(s) 737
AcyI GRCGYC 1 cut(s) 550
AfiI CCNNNNNNNGG 4 cut(s) 239, 600, 606, 622
AjnI CCWGG 2 cut(s) 255, 648
AluBI AGCT 9 cut(s) 355, 734, 746, 794, 916, 966, 1000, 1046, 1067
AluI AGCT 9 cut(s) 355, 734, 746, 794, 916, 966, 1000, 1046, 1067
Alw21I GWGCWC 4 cut(s) 264, 357, 796, 968
AlwI GGATC 4 cut(s) 30, 201, 702, 1007
Ama87I CYCGRG 2 cut(s) 329, 594
AoxI GGCC 5 cut(s) 3, 209, 597, 630, 646
ApaI GGGCCC 1 cut(s) 601
ApeKI GCWGC 7 cut(s) 70, 310, 565, 731, 812, 916, 1000
ApoI RAATTY 1 cut(s) 973
ArsI GACNNNNNNTTYG 2 cut(s) 221, 253
Asp700I GAANNNNTTC 2 cut(s) 167, 180
AspLEI GCGC 5 cut(s) 70, 108, 277, 286, 394
AspS9I GGNCC 4 cut(s) 597, 598, 630, 646
AsuC2I CCSGG 6 cut(s) 137, 208, 595, 596, 601, 983
AsuHPI GGTGA 2 cut(s) 911, 953
AsuII TTCGAA 1 cut(s) 806
AvaI CYCGRG 2 cut(s) 329, 594
AxyI CCTNAGG 2 cut(s) 344, 714
BaeGI GKGCMC 1 cut(s) 601
BanII GRGCYC 4 cut(s) 357, 601, 796, 968
BbsI GAAGAC 1 cut(s) 543
Bbv12I GWGCWC 4 cut(s) 264, 357, 796, 968
BbvI GCAGC 7 cut(s) 82, 297, 552, 743, 824, 903, 987
BccI CCATC 3 cut(s) 22, 637, 671
BceAI ACGGC 3 cut(s) 196, 376, 800
BcgI CGANNNNNNTGC 2 cut(s) 801, 835
BciT130I CCWGG 2 cut(s) 257, 650
BcnI CCSGG 6 cut(s) 137, 208, 595, 596, 601, 983
BfaI CTAG 1 cut(s) 969
BfuAI ACCTGC 1 cut(s) 549
BglI GCCNNNNNGGC 1 cut(s) 925
BglII AGATCT 2 cut(s) 482, 636
BlpI GCTNAGC 1 cut(s) 962
Bme1390I CCNGG 8 cut(s) 137, 208, 257, 595, 596, 601, 650, 983
BmeT110I CYCGRG 2 cut(s) 329, 594
BmgT120I GGNCC 4 cut(s) 597, 598, 630, 646
BmiI GGNNCC 3 cut(s) 244, 599, 614
BmrFI CCNGG 8 cut(s) 137, 208, 257, 595, 596, 601, 650, 983
BmsI GCATC 2 cut(s) 585, 672
BpiI GAAGAC 1 cut(s) 543
BplI GAGNNNNNCTC 4 cut(s) 644, 676, 778, 810
BpmI CTGGAG 2 cut(s) 239, 671
Bpu1102I GCTNAGC 1 cut(s) 962
Bpu14I TTCGAA 1 cut(s) 806
BpuMI CCSGG 6 cut(s) 137, 208, 595, 596, 601, 983
Bsa29I ATCGAT 1 cut(s) 179
BsaAI YACGTR 1 cut(s) 703
BsaHI GRCGYC 1 cut(s) 550
BsaI GGTCTC 2 cut(s) 47, 761
BsaJI CCNNGG 4 cut(s) 330, 594, 759, 956
BsaWI WCCGGW 1 cut(s) 615
BsaXI ACNNNNNCTCC 4 cut(s) 71, 101, 830, 860
Bsc4I CCNNNNNNNGG 4 cut(s) 239, 600, 606, 622
Bse118I RCCGGY 2 cut(s) 799, 990
Bse21I CCTNAGG 2 cut(s) 344, 714
Bse3DI GCAATG 1 cut(s) 585
BseBI CCWGG 2 cut(s) 257, 650
BseCI ATCGAT 1 cut(s) 179
BseDI CCNNGG 4 cut(s) 330, 594, 759, 956
BseGI GGATG 2 cut(s) 196, 859
BseLI CCNNNNNNNGG 4 cut(s) 239, 600, 606, 622
BseMI GCAATG 1 cut(s) 585
BseMII CTCAG 5 cut(s) 32, 329, 358, 705, 953
BseSI GKGCMC 1 cut(s) 601
BseX3I CGGCCG 1 cut(s) 209
BseXI GCAGC 7 cut(s) 82, 297, 552, 743, 824, 903, 987
BseYI CCCAGC 2 cut(s) 363, 734
Bsh1236I CGCG 7 cut(s) 34, 108, 275, 286, 392, 492, 558
Bsh1285I CGRYCG 2 cut(s) 126, 212
BshFI GGCC 5 cut(s) 5, 211, 599, 632, 648
BshVI ATCGAT 1 cut(s) 179
BsiEI CGRYCG 2 cut(s) 126, 212
BsiHKAI GWGCWC 4 cut(s) 264, 357, 796, 968
BsiHKCI CYCGRG 2 cut(s) 329, 594
BslFI GGGAC 1 cut(s) 380
BslI CCNNNNNNNGG 4 cut(s) 239, 600, 606, 622
BsmBI CGTCTC 6 cut(s) 46, 53, 113, 115, 522, 575
BsmFI GGGAC 1 cut(s) 380
BsnI GGCC 5 cut(s) 5, 211, 599, 632, 648
Bso31I GGTCTC 2 cut(s) 47, 761
BsoBI CYCGRG 2 cut(s) 329, 594
Bsp119I TTCGAA 1 cut(s) 806
Bsp120I GGGCCC 1 cut(s) 597
Bsp1286I GDGCHC 5 cut(s) 264, 357, 601, 796, 968
Bsp1720I GCTNAGC 1 cut(s) 962
Bsp19I CCATGG 1 cut(s) 956
Bsp68I TCGCGA 2 cut(s) 492, 558
BspANI GGCC 5 cut(s) 5, 211, 599, 632, 648
BspCNI CTCAG 5 cut(s) 31, 328, 357, 706, 954
BspDI ATCGAT 1 cut(s) 179
BspFNI CGCG 7 cut(s) 34, 108, 275, 286, 392, 492, 558
BspLI GGNNCC 3 cut(s) 244, 599, 614
BspMI ACCTGC 1 cut(s) 549
BspPI GGATC 4 cut(s) 30, 201, 702, 1007
BspT104I TTCGAA 1 cut(s) 806
BspTNI GGTCTC 2 cut(s) 47, 761
BsrBI CCGCTC 1 cut(s) 606
BsrDI GCAATG 1 cut(s) 585
BsrFI RCCGGY 2 cut(s) 799, 990
BssAI RCCGGY 2 cut(s) 799, 990
BssECI CCNNGG 4 cut(s) 330, 594, 759, 956
BssNI GRCGYC 1 cut(s) 550
BssT1I CCWWGG 2 cut(s) 759, 956
Bst2UI CCWGG 2 cut(s) 257, 650
Bst4CI ACNGT 3 cut(s) 124, 189, 675
Bst6I CTCTTC 3 cut(s) 438, 468, 495
BstACI GRCGYC 1 cut(s) 550
BstBAI YACGTR 1 cut(s) 703
BstBI TTCGAA 1 cut(s) 806
BstC8I GCNNGC 4 cut(s) 279, 831, 926, 1044
BstDEI CTNAG 5 cut(s) 18, 315, 344, 714, 962
BstDSI CCRYGG 1 cut(s) 956
BstF5I GGATG 2 cut(s) 196, 859
BstFNI CGCG 7 cut(s) 34, 108, 275, 286, 392, 492, 558
BstHHI GCGC 5 cut(s) 70, 108, 277, 286, 394
BstMCI CGRYCG 2 cut(s) 126, 212
BstNI CCWGG 2 cut(s) 257, 650
BstSCI CCNGG 8 cut(s) 135, 206, 255, 593, 594, 599, 648, 981
BstSLI GKGCMC 1 cut(s) 601
BstSNI TACGTA 1 cut(s) 703
BstUI CGCG 7 cut(s) 34, 108, 275, 286, 392, 492, 558
BstV1I GCAGC 7 cut(s) 82, 297, 552, 743, 824, 903, 987
BstV2I GAAGAC 1 cut(s) 543
BstX2I RGATCY 2 cut(s) 482, 636
BstYI RGATCY 2 cut(s) 482, 636
BstZI CGGCCG 1 cut(s) 209
Bsu15I ATCGAT 1 cut(s) 179
Bsu36I CCTNAGG 2 cut(s) 344, 714
BsuRI GGCC 5 cut(s) 5, 211, 599, 632, 648
BsuTUI ATCGAT 1 cut(s) 179
BtgI CCRYGG 1 cut(s) 956
BtgZI GCGATG 1 cut(s) 573
BtsCI GGATG 2 cut(s) 196, 859
BtsI GCAGTG 2 cut(s) 94, 784
BtsIMutI CAGTG 2 cut(s) 94, 784
BtuMI TCGCGA 2 cut(s) 492, 558
BveI ACCTGC 1 cut(s) 549
Cac8I GCNNGC 4 cut(s) 279, 831, 926, 1044
CfoI GCGC 5 cut(s) 70, 108, 277, 286, 394
Cfr10I RCCGGY 2 cut(s) 799, 990
Cfr13I GGNCC 4 cut(s) 597, 598, 630, 646
Cfr9I CCCGGG 1 cut(s) 594
ClaI ATCGAT 1 cut(s) 179
CseI GACGC 5 cut(s) 97, 388, 398, 557, 737
CviAII CATG 4 cut(s) 921, 929, 938, 957
DdeI CTNAG 5 cut(s) 18, 315, 344, 714, 962
DrdI GACNNNNNNGTC 1 cut(s) 552
DseDI GACNNNNNNGTC 1 cut(s) 552
EaeI YGGCCR 2 cut(s) 3, 209
EagI CGGCCG 1 cut(s) 209
Eam1104I CTCTTC 3 cut(s) 438, 468, 495
EarI CTCTTC 3 cut(s) 438, 468, 495
EciI GGCGGA 3 cut(s) 239, 455, 779
Ecl136II GAGCTC 3 cut(s) 355, 794, 966
EclXI CGGCCG 1 cut(s) 209
Eco105I TACGTA 1 cut(s) 703
Eco130I CCWWGG 2 cut(s) 759, 956
Eco24I GRGCYC 4 cut(s) 357, 601, 796, 968
Eco31I GGTCTC 2 cut(s) 47, 761
Eco32I GATATC 1 cut(s) 935
Eco52I CGGCCG 1 cut(s) 209
Eco53kI GAGCTC 3 cut(s) 355, 794, 966
Eco57I CTGAAG 1 cut(s) 737
Eco81I CCTNAGG 2 cut(s) 344, 714
Eco88I CYCGRG 2 cut(s) 329, 594
EcoICRI GAGCTC 3 cut(s) 355, 794, 966
EcoRI GAATTC 1 cut(s) 973
EcoRII CCWGG 2 cut(s) 255, 648
EcoRV GATATC 1 cut(s) 935
EcoT14I CCWWGG 2 cut(s) 759, 956
EcoT38I GRGCYC 4 cut(s) 357, 601, 796, 968
ErhI CCWWGG 2 cut(s) 759, 956
Esp3I CGTCTC 6 cut(s) 46, 53, 113, 115, 522, 575
FaeI CATG 4 cut(s) 924, 932, 941, 960
FaiI YATR 8 cut(s) 741, 875, 877, 887, 922, 930, 939, 958
FalI AAGNNNNNCTT 2 cut(s) 735, 767
FaqI GGGAC 1 cut(s) 380
FatI CATG 4 cut(s) 920, 928, 937, 956
FauI CCCGC 3 cut(s) 312, 599, 917
FauNDI CATATG 1 cut(s) 875
FokI GGATG 2 cut(s) 183, 866
FriOI GRGCYC 4 cut(s) 357, 601, 796, 968
FspBI CTAG 1 cut(s) 969
GlaI GCGC 5 cut(s) 69, 107, 276, 285, 393
GsaI CCCAGC 2 cut(s) 367, 738
GsuI CTGGAG 2 cut(s) 239, 671
HaeIII GGCC 5 cut(s) 5, 211, 599, 632, 648
HgaI GACGC 5 cut(s) 97, 388, 398, 557, 737
HhaI GCGC 5 cut(s) 70, 108, 277, 286, 394
Hin1I GRCGYC 1 cut(s) 550
Hin1II CATG 4 cut(s) 924, 932, 941, 960
Hin6I GCGC 5 cut(s) 68, 106, 275, 284, 392
HinP1I GCGC 5 cut(s) 68, 106, 275, 284, 392
HincII GTYRAC 1 cut(s) 727
HindII GTYRAC 1 cut(s) 727
HinfI GANTC 5 cut(s) 176, 181, 505, 619, 689
HphI GGTGA 2 cut(s) 911, 953
Hpy166II GTNNAC 1 cut(s) 727
Hpy188III TCNNGA 9 cut(s) 118, 346, 491, 557, 623, 640, 679, 713, 1016
Hpy8I GTNNAC 1 cut(s) 727
HpyAV CCTTC 3 cut(s) 247, 410, 777
HpyCH4III ACNGT 3 cut(s) 124, 189, 675
HpyCH4IV ACGT 3 cut(s) 550, 702, 723
HpyCH4V TGCA 4 cut(s) 99, 159, 879, 948
HpyF3I CTNAG 5 cut(s) 18, 315, 344, 714, 962
HpySE526I ACGT 3 cut(s) 550, 702, 723
Hsp92I GRCGYC 1 cut(s) 550
Hsp92II CATG 4 cut(s) 924, 932, 941, 960
HspAI GCGC 5 cut(s) 68, 106, 275, 284, 392
LmnI GCTCC 6 cut(s) 248, 267, 603, 791, 799, 1064
Lsp1109I GCAGC 7 cut(s) 82, 297, 552, 743, 824, 903, 987
LweI GCATC 2 cut(s) 585, 672
MaeI CTAG 1 cut(s) 969
MaeII ACGT 3 cut(s) 550, 702, 723
MaeIII GTNAC 2 cut(s) 698, 941
MbiI CCGCTC 1 cut(s) 606
MboII GAAGA 9 cut(s) 347, 455, 479, 482, 485, 512, 545, 548, 1048
MflI RGATCY 2 cut(s) 482, 636
MhlI GDGCHC 5 cut(s) 264, 357, 601, 796, 968
MluCI AATT 4 cut(s) 668, 824, 973, 1087
MmeI TCCRAC 2 cut(s) 69, 171
MroXI GAANNNNTTC 2 cut(s) 167, 180
MseI TTAA 2 cut(s) 843, 891
MspA1I CMGCKG 3 cut(s) 310, 734, 833
MspR9I CCNGG 8 cut(s) 137, 208, 257, 595, 596, 601, 650, 983
MvaI CCWGG 2 cut(s) 257, 650
MvnI CGCG 7 cut(s) 34, 108, 275, 286, 392, 492, 558
NciI CCSGG 6 cut(s) 137, 208, 595, 596, 601, 983
NcoI CCATGG 1 cut(s) 956
NdeI CATATG 1 cut(s) 875
NlaIII CATG 4 cut(s) 924, 932, 941, 960
NlaIV GGNNCC 3 cut(s) 244, 599, 614
NmeAIII GCCGAG 1 cut(s) 658
NmuCI GTSAC 1 cut(s) 941
NruI TCGCGA 2 cut(s) 492, 558
NspV TTCGAA 1 cut(s) 806
PcsI WCGNNNNNNNCGW 7 cut(s) 150, 185, 378, 381, 396, 523, 684
PdmI GAANNNNTTC 2 cut(s) 167, 180
PfeI GAWTC 5 cut(s) 176, 181, 505, 619, 689
PflFI GACNNNGTC 3 cut(s) 143, 549, 552
PfoI TCCNGGA 1 cut(s) 135
Ppu21I YACGTR 1 cut(s) 703
Psp124BI GAGCTC 3 cut(s) 357, 796, 968
Psp6I CCWGG 2 cut(s) 255, 648
PspFI CCCAGC 2 cut(s) 363, 734
PspGI CCWGG 2 cut(s) 255, 648
PspN4I GGNNCC 3 cut(s) 244, 599, 614
PspOMI GGGCCC 1 cut(s) 597
PspPI GGNCC 4 cut(s) 597, 598, 630, 646
PsuI RGATCY 2 cut(s) 482, 636
PsyI GACNNNGTC 3 cut(s) 143, 549, 552
PvuII CAGCTG 1 cut(s) 734
RruI TCGCGA 2 cut(s) 492, 558
SacI GAGCTC 3 cut(s) 357, 796, 968
SaqAI TTAA 2 cut(s) 843, 891
Sau96I GGNCC 4 cut(s) 597, 598, 630, 646
ScrFI CCNGG 8 cut(s) 137, 208, 257, 595, 596, 601, 650, 983
SduI GDGCHC 5 cut(s) 264, 357, 601, 796, 968
SfaNI GCATC 2 cut(s) 585, 672
SfuI TTCGAA 1 cut(s) 806
SmaI CCCGGG 1 cut(s) 596
SnaBI TACGTA 1 cut(s) 703
Sse9I AATT 4 cut(s) 668, 824, 973, 1087
SspMI CTAG 1 cut(s) 969
SstI GAGCTC 3 cut(s) 357, 796, 968
StyD4I CCNGG 8 cut(s) 135, 206, 255, 593, 594, 599, 648, 981
StyI CCWWGG 2 cut(s) 759, 956
TaaI ACNGT 3 cut(s) 124, 189, 675
TaiI ACGT 3 cut(s) 553, 705, 726
TaqI TCGA 7 cut(s) 117, 179, 357, 402, 666, 678, 806
TaqII GACCGA 2 cut(s) 71, 785
TasI AATT 4 cut(s) 668, 824, 973, 1087
TauI GCSGC 7 cut(s) 28, 31, 34, 76, 284, 310, 423
TfiI GAWTC 5 cut(s) 176, 181, 505, 619, 689
Tru1I TTAA 2 cut(s) 843, 891
Tru9I TTAA 2 cut(s) 843, 891
TscAI CASTG 2 cut(s) 101, 784
TseFI GTSAC 1 cut(s) 941
TseI GCWGC 7 cut(s) 70, 310, 565, 731, 812, 916, 1000
Tsp45I GTSAC 1 cut(s) 941
TspDTI ATGAA 2 cut(s) 429, 756
TspGWI ACGGA 5 cut(s) 28, 65, 77, 103, 254
TspMI CCCGGG 1 cut(s) 594
TspRI CASTG 2 cut(s) 101, 784
Tth111I GACNNNGTC 3 cut(s) 143, 549, 552
XapI RAATTY 1 cut(s) 973
XmaI CCCGGG 1 cut(s) 594
XmnI GAANNNNTTC 2 cut(s) 167, 180
XspI CTAG 1 cut(s) 969
ZraI GACGTC 1 cut(s) 551
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.