MD08G1011000.v1.1

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0014025
Physical Location & Seq
Reverse (-)
848971 .. 851368
2398 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1011000.v1.1.491

Sequence Viewer

Length: 1125 bp
ATGGCTGAAACTCCATCCCAGACGCCGCCGCCGCCGCCGCGATCTGCGTCTGAGGCCGAGACGGAGACGATGGAGTACTGGTGCTACCACTGCAACAAACGCGTCTCCATCGAAACCGTGGCGAATCTCCCTGACATCGTTTGCCACGAGTGCAAGAATGGTTTCGTCGAATCCATCCCGGCCGCGTCATATCCGCAGTGGGATCCTCCTTCCCGATCGTCCGATCAGGTCGATGACCCGACTTTCGGCTCTCCATTTCTCCAGGTGCTGAGGCTAATCGCTCAGGCGGCGCGTGAGGAGGATGCGCCCCCGCCGCAGCCTGAAAACCCCTCGCTTGATGACGATTTCTTTAGGATTGAGATGGAGGGGTGGGACAACGACGAGGACGAGGACGACGAAGATGCGCACAGTGTCGAGTTTCACAACCACGACGGCGAGGATAACGAAGAAGCTGGAGAGGAAGTAGAAGAGGAGGAGGAGGAGGAAGAAGAAGAAGATAGATCGGATAACGAGGATGAAGAGAATCAGGAAGATCAGCAAGAGAGAGATGAAGAAGACATGCGACGACATCGTCGAGACGTGCTTCGTCTCCACATCCGAGAGATTGCAACCCGAGCGAGCAGCGGGAGGAACCGGATTCTGGATTGGGCCGAGATCTTGATGGGGCTGGAGGACAATTCGATCGAATTCCGGCTCGAAATGCCCGAATCGGACCGGTACATCGGCAATCCCGAGGATTACGTGGACGCAGCCGGCTATGAGGCTTTGCTGCAGACCTTGGCTGAGAGCGATAATGCTGGAAGGAGAGGGGCTCCACCGGCGGGGAAGAATGCTGTGTCGGAGTTACCATCGGTCAAGATTGCGGCAGAGGATGAGGCCGTGGTGTGCGCCATATGCAAGGACATGGTGAATGTTGGTGACATGTCGAAGAAGCTGCCGTGTGGGCATGGTTACCACGGCGATTGCATCGTTCCTTGGCTGAGTTCCAGAAATACTTGCCCGGTTTGCCGGTTCGAGCTGCCAACCGATGATCCGGAATACGAGGAGGAGAGGAAGAAGCGAGCTGTGTCTGTTAGCGCGGCTGGAGCTTCCAGTTCTGGCGGAGATAATTCAATCTCTCATTGA

Protein Analysis

375

Amino Acids

42.0

Weight (kDa)

4.24

Isoelectric Point (pI)

75.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zn_ribbon_19 PF14369 26 - 59 4.6e-10 zinc-ribbon
zf-RING_2 PF13639 296 - 337 1.6e-12 Ring finger domain
zf-C3HC4_2 PF13923 296 - 336 2.2e-10 Zinc finger, C3HC4 type (RING finger)
zf-RING_11 PF17123 296 - 323 1e-07 RING-like zinc finger
zf-C3HC4 PF00097 296 - 336 6.8e-08 Zinc finger, C3HC4 type (RING finger)
zf-rbx1 PF12678 313 - 337 7.3e-07 RING-H2 zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014025)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G64920
fragaria_vesca FvH4_2g38280
malus_domestica MD08G1011000.v1.1 MD15G1011000.v1.1
prunus_persica Prupe.1G363700_v2.0.a1
pyrus_communis pycom15g00940
rosa_chinensis RchiOBHm_Chr6g0303101
rosa_laevigata RLG00000011108
rosa_multiflora Rmu_sc0003721.1_g000001 Rmu_sc0008911.1_g000004
rosa_roxburghii Rroxscaffold_7G00165020
rosa_rugosa Rorug06G0319200
rosa_samantha Rh6AG433000 Rh6BG469700 Rh6CG445300 Rh6DG432100
rosa_wichuraiana Rw6G037530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 570
Acc16I TGCGCA 1 cut(s) 405
AccII CGCG 5 cut(s) 40, 102, 185, 292, 1079
AccIII TCCGGA 1 cut(s) 1033
AclWI GGATC 3 cut(s) 197, 210, 1025
AcoI YGGCCR 1 cut(s) 180
AcsI RAATTY 1 cut(s) 686
AcyI GRCGYC 1 cut(s) 23
AfaI GTAC 2 cut(s) 77, 719
AfiI CCNNNNNNNGG 3 cut(s) 245, 640, 821
AflIII ACRYGT 2 cut(s) 100, 921
AgeI ACCGGT 1 cut(s) 714
AgsI TTSAA 1 cut(s) 1113
AjiI CACGTC 1 cut(s) 580
AjnI CCWGG 1 cut(s) 261
AluBI AGCT 5 cut(s) 452, 934, 1018, 1064, 1088
AluI AGCT 5 cut(s) 452, 934, 1018, 1064, 1088
Alw26I GTCTC 5 cut(s) 53, 59, 109, 570, 593
AlwI GGATC 3 cut(s) 197, 210, 1025
AlwNI CAGNNNCTG 1 cut(s) 268
Ama87I CYCGRG 2 cut(s) 612, 731
Aor13HI TCCGGA 1 cut(s) 1033
AoxI GGCC 4 cut(s) 54, 180, 648, 876
ApeKI GCWGC 6 cut(s) 316, 621, 749, 769, 934, 1018
ApoI RAATTY 1 cut(s) 686
ArsI GACNNNNNNTTYG 2 cut(s) 227, 259
AsiGI ACCGGT 1 cut(s) 714
Asp700I GAANNNNTTC 1 cut(s) 161
AspLEI GCGC 5 cut(s) 292, 307, 406, 890, 1079
AspS9I GGNCC 2 cut(s) 648, 712
AsuC2I CCSGG 2 cut(s) 179, 1001
AsuHPI GGTGA 2 cut(s) 919, 929
AvaI CYCGRG 2 cut(s) 612, 731
AvaII GGWCC 1 cut(s) 712
BamHI GGATCC 1 cut(s) 202
BanII GRGCYC 1 cut(s) 814
BauI CACGAG 1 cut(s) 146
BbsI GAAGAC 1 cut(s) 561
BbvCI CCTCAGC 1 cut(s) 269
BbvI GCAGC 6 cut(s) 328, 633, 756, 761, 921, 1005
BccI CCATC 7 cut(s) 22, 64, 116, 182, 355, 655, 856
BceAI ACGGC 4 cut(s) 448, 863, 922, 973
BcgI CGANNNNNNTGC 4 cut(s) 383, 417, 916, 950
BciT130I CCWGG 1 cut(s) 263
BcnI CCSGG 2 cut(s) 179, 1001
BcoDI GTCTC 5 cut(s) 53, 59, 109, 570, 593
BfmI CTRYAG 1 cut(s) 770
BglI GCCNNNNNGGC 1 cut(s) 943
BglII AGATCT 1 cut(s) 654
BmcAI AGTACT 1 cut(s) 77
Bme1390I CCNGG 3 cut(s) 179, 263, 1001
Bme18I GGWCC 1 cut(s) 712
BmeT110I CYCGRG 2 cut(s) 612, 731
BmgBI CACGTC 1 cut(s) 580
BmgT120I GGNCC 2 cut(s) 648, 712
BmiI GGNNCC 3 cut(s) 204, 632, 813
BmrFI CCNGG 3 cut(s) 179, 263, 1001
BmsI GCATC 3 cut(s) 292, 391, 975
BpiI GAAGAC 1 cut(s) 561
BplI GAGNNNNNCTC 2 cut(s) 796, 828
BpmI CTGGAG 4 cut(s) 245, 474, 689, 1104
Bpu10I CCTNAGC 2 cut(s) 269, 282
BpuMI CCSGG 2 cut(s) 179, 1001
BsaAI YACGTR 1 cut(s) 742
BsaHI GRCGYC 1 cut(s) 23
BsaJI CCNNGG 6 cut(s) 117, 732, 777, 879, 955, 974
BsaWI WCCGGW 3 cut(s) 633, 714, 1033
BsaXI ACNNNNNCTCC 4 cut(s) 65, 95, 447, 477
Bsc4I CCNNNNNNNGG 3 cut(s) 245, 640, 821
Bse118I RCCGGY 4 cut(s) 714, 752, 817, 1008
Bse1I ACTGG 2 cut(s) 83, 1092
BseAI TCCGGA 1 cut(s) 1033
BseBI CCWGG 1 cut(s) 263
BseDI CCNNGG 6 cut(s) 117, 732, 777, 879, 955, 974
BseGI GGATG 6 cut(s) 14, 174, 307, 520, 594, 877
BseLI CCNNNNNNNGG 3 cut(s) 245, 640, 821
BseMII CTCAG 5 cut(s) 42, 260, 296, 774, 971
BseNI ACTGG 2 cut(s) 83, 1092
BseRI GAGGAG 7 cut(s) 311, 485, 488, 491, 494, 1058, 1061
BseX3I CGGCCG 1 cut(s) 180
BseXI GCAGC 6 cut(s) 328, 633, 756, 761, 921, 1005
Bsh1236I CGCG 5 cut(s) 40, 102, 185, 292, 1079
Bsh1285I CGRYCG 3 cut(s) 183, 218, 684
BshFI GGCC 4 cut(s) 56, 182, 650, 878
BshTI ACCGGT 1 cut(s) 714
BsiEI CGRYCG 3 cut(s) 183, 218, 684
BsiHKCI CYCGRG 2 cut(s) 612, 731
BsiSI CCGG 9 cut(s) 179, 634, 691, 715, 753, 818, 1001, 1009, 1034
BslFI GGGAC 1 cut(s) 386
BslI CCNNNNNNNGG 3 cut(s) 245, 640, 821
BsmAI GTCTC 5 cut(s) 53, 59, 109, 570, 593
BsmBI CGTCTC 5 cut(s) 53, 59, 109, 570, 593
BsmFI GGGAC 1 cut(s) 386
BsmI GAATGC 1 cut(s) 835
BsnI GGCC 4 cut(s) 56, 182, 650, 878
BsoBI CYCGRG 2 cut(s) 612, 731
Bsp1286I GDGCHC 1 cut(s) 814
Bsp13I TCCGGA 1 cut(s) 1033
Bsp143I GATC 9 cut(s) 41, 202, 215, 223, 500, 532, 654, 681, 1030
BspANI GGCC 4 cut(s) 56, 182, 650, 878
BspCNI CTCAG 5 cut(s) 43, 261, 295, 775, 972
BspEI TCCGGA 1 cut(s) 1033
BspFNI CGCG 5 cut(s) 40, 102, 185, 292, 1079
BspLI GGNNCC 3 cut(s) 204, 632, 813
BspMAI CTGCAG 1 cut(s) 774
BspPI GGATC 3 cut(s) 197, 210, 1025
BsrFI RCCGGY 4 cut(s) 714, 752, 817, 1008
BsrI ACTGG 2 cut(s) 83, 1092
BssAI RCCGGY 4 cut(s) 714, 752, 817, 1008
BssECI CCNNGG 6 cut(s) 117, 732, 777, 879, 955, 974
BssMI GATC 9 cut(s) 41, 202, 215, 223, 500, 532, 654, 681, 1030
BssNI GRCGYC 1 cut(s) 23
BssSI CACGAG 1 cut(s) 146
BssT1I CCWWGG 2 cut(s) 777, 974
Bst2BI CACGAG 1 cut(s) 146
Bst2UI CCWGG 1 cut(s) 263
Bst4CI ACNGT 2 cut(s) 118, 410
Bst6I CTCTTC 2 cut(s) 462, 513
BstACI GRCGYC 1 cut(s) 23
BstBAI YACGTR 1 cut(s) 742
BstC8I GCNNGC 3 cut(s) 619, 754, 1062
BstDEI CTNAG 5 cut(s) 51, 269, 282, 783, 980
BstDSI CCRYGG 3 cut(s) 117, 879, 955
BstEII GGTNACC 1 cut(s) 950
BstF5I GGATG 6 cut(s) 14, 174, 307, 520, 594, 877
BstFNI CGCG 5 cut(s) 40, 102, 185, 292, 1079
BstHHI GCGC 5 cut(s) 292, 307, 406, 890, 1079
BstKTI GATC 9 cut(s) 44, 205, 218, 226, 503, 535, 657, 684, 1033
BstMAI GTCTC 5 cut(s) 53, 59, 109, 570, 593
BstMBI GATC 9 cut(s) 41, 202, 215, 223, 500, 532, 654, 681, 1030
BstMCI CGRYCG 3 cut(s) 183, 218, 684
BstNI CCWGG 1 cut(s) 263
BstNSI RCATGY 2 cut(s) 562, 925
BstPI GGTNACC 1 cut(s) 950
BstSCI CCNGG 3 cut(s) 177, 261, 999
BstSFI CTRYAG 1 cut(s) 770
BstUI CGCG 5 cut(s) 40, 102, 185, 292, 1079
BstV1I GCAGC 6 cut(s) 328, 633, 756, 761, 921, 1005
BstV2I GAAGAC 1 cut(s) 561
BstX2I RGATCY 2 cut(s) 202, 654
BstYI RGATCY 2 cut(s) 202, 654
BstZI CGGCCG 1 cut(s) 180
BsuRI GGCC 4 cut(s) 56, 182, 650, 878
BtgI CCRYGG 3 cut(s) 117, 879, 955
BtrI CACGTC 1 cut(s) 580
BtsCI GGATG 6 cut(s) 14, 174, 307, 520, 594, 877
BtsI GCAGTG 2 cut(s) 88, 203
BtsIMutI CAGTG 3 cut(s) 88, 203, 415
Cac8I GCNNGC 3 cut(s) 619, 754, 1062
CaiI CAGNNNCTG 1 cut(s) 268
CfoI GCGC 5 cut(s) 292, 307, 406, 890, 1079
Cfr10I RCCGGY 4 cut(s) 714, 752, 817, 1008
Cfr13I GGNCC 2 cut(s) 648, 712
CpoI CGGWCCG 1 cut(s) 712
CseI GACGC 5 cut(s) 31, 36, 91, 174, 755
Csp6I GTAC 2 cut(s) 76, 718
CspAI ACCGGT 1 cut(s) 714
CspCI CAANNNNNGTGG 2 cut(s) 944, 979
CspI CGGWCCG 1 cut(s) 712
CviAII CATG 4 cut(s) 559, 904, 922, 947
CviQI GTAC 2 cut(s) 76, 718
DdeI CTNAG 5 cut(s) 51, 269, 282, 783, 980
DpnI GATC 9 cut(s) 43, 204, 217, 225, 502, 534, 656, 683, 1032
DpnII GATC 9 cut(s) 41, 202, 215, 223, 500, 532, 654, 681, 1030
DrdI GACNNNNNNGTC 1 cut(s) 570
DseDI GACNNNNNNGTC 1 cut(s) 570
EaeI YGGCCR 1 cut(s) 180
EagI CGGCCG 1 cut(s) 180
Eam1104I CTCTTC 2 cut(s) 462, 513
EarI CTCTTC 2 cut(s) 462, 513
EciI GGCGGA 1 cut(s) 1116
EclXI CGGCCG 1 cut(s) 180
Eco130I CCWWGG 2 cut(s) 777, 974
Eco24I GRGCYC 1 cut(s) 814
Eco47I GGWCC 1 cut(s) 712
Eco52I CGGCCG 1 cut(s) 180
Eco88I CYCGRG 2 cut(s) 612, 731
Eco91I GGTNACC 1 cut(s) 950
EcoO65I GGTNACC 1 cut(s) 950
EcoRI GAATTC 1 cut(s) 686
EcoRII CCWGG 1 cut(s) 261
EcoT14I CCWWGG 2 cut(s) 777, 974
EcoT38I GRGCYC 1 cut(s) 814
ErhI CCWWGG 2 cut(s) 777, 974
Esp3I CGTCTC 5 cut(s) 53, 59, 109, 570, 593
FaeI CATG 4 cut(s) 562, 907, 925, 950
FaiI YATR 8 cut(s) 190, 560, 759, 893, 895, 905, 923, 948
FaqI GGGAC 1 cut(s) 386
FatI CATG 4 cut(s) 558, 903, 921, 946
FauI CCCGC 3 cut(s) 318, 617, 814
FauNDI CATATG 1 cut(s) 893
FokI GGATG 5 cut(s) 161, 314, 527, 581, 884
FriOI GRGCYC 1 cut(s) 814
FspAI RTGCGCAY 1 cut(s) 405
FspI TGCGCA 1 cut(s) 405
GlaI GCGC 5 cut(s) 291, 306, 405, 889, 1078
GsuI CTGGAG 4 cut(s) 245, 474, 689, 1104
HaeIII GGCC 4 cut(s) 56, 182, 650, 878
HapII CCGG 9 cut(s) 179, 634, 691, 715, 753, 818, 1001, 1009, 1034
HgaI GACGC 5 cut(s) 31, 36, 91, 174, 755
HhaI GCGC 5 cut(s) 292, 307, 406, 890, 1079
Hin1I GRCGYC 1 cut(s) 23
Hin1II CATG 4 cut(s) 562, 907, 925, 950
Hin6I GCGC 5 cut(s) 290, 305, 404, 888, 1077
HinP1I GCGC 5 cut(s) 290, 305, 404, 888, 1077
HinfI GANTC 5 cut(s) 124, 170, 523, 637, 707
HpaII CCGG 9 cut(s) 179, 634, 691, 715, 753, 818, 1001, 1009, 1034
HphI GGTGA 2 cut(s) 919, 929
Hpy166II GTNNAC 1 cut(s) 745
Hpy188I TCNGA 6 cut(s) 52, 223, 505, 599, 712, 841
Hpy188III TCNNGA 9 cut(s) 213, 527, 575, 641, 658, 731, 856, 987, 1034
Hpy8I GTNNAC 1 cut(s) 745
Hpy99I CGWCG 6 cut(s) 170, 383, 398, 434, 567, 576
HpyAV CCTTC 2 cut(s) 219, 795
HpyCH4III ACNGT 2 cut(s) 118, 410
HpyCH4IV ACGT 2 cut(s) 579, 741
HpyCH4V TGCA 6 cut(s) 93, 153, 608, 772, 897, 966
HpyF3I CTNAG 5 cut(s) 51, 269, 282, 783, 980
HpySE526I ACGT 2 cut(s) 579, 741
Hsp92I GRCGYC 1 cut(s) 23
Hsp92II CATG 4 cut(s) 562, 907, 925, 950
HspAI GCGC 5 cut(s) 290, 305, 404, 888, 1077
Kpn2I TCCGGA 1 cut(s) 1033
KroI GCCGGC 1 cut(s) 752
KroNI GCCGGC 1 cut(s) 754
Kzo9I GATC 9 cut(s) 41, 202, 215, 223, 500, 532, 654, 681, 1030
LmnI GCTCC 2 cut(s) 817, 1085
Lsp1109I GCAGC 6 cut(s) 328, 633, 756, 761, 921, 1005
LweI GCATC 3 cut(s) 292, 391, 975
MaeII ACGT 2 cut(s) 579, 741
MaeIII GTNAC 3 cut(s) 843, 917, 950
MalI GATC 9 cut(s) 43, 204, 217, 225, 502, 534, 656, 683, 1032
MboI GATC 9 cut(s) 41, 202, 215, 223, 500, 532, 654, 681, 1030
MflI RGATCY 2 cut(s) 202, 654
MhlI GDGCHC 1 cut(s) 814
MluCI AATT 3 cut(s) 676, 686, 1108
MluI ACGCGT 1 cut(s) 100
MmeI TCCRAC 1 cut(s) 819
MroI TCCGGA 1 cut(s) 1033
MroNI GCCGGC 1 cut(s) 752
MroXI GAANNNNTTC 1 cut(s) 161
MspA1I CMGCKG 1 cut(s) 624
MspI CCGG 9 cut(s) 179, 634, 691, 715, 753, 818, 1001, 1009, 1034
MspR9I CCNGG 3 cut(s) 179, 263, 1001
Mva1269I GAATGC 1 cut(s) 835
MvaI CCWGG 1 cut(s) 263
MvnI CGCG 5 cut(s) 40, 102, 185, 292, 1079
NaeI GCCGGC 1 cut(s) 754
NciI CCSGG 2 cut(s) 179, 1001
NdeI CATATG 1 cut(s) 893
NdeII GATC 9 cut(s) 41, 202, 215, 223, 500, 532, 654, 681, 1030
NgoMIV GCCGGC 1 cut(s) 752
NlaIII CATG 4 cut(s) 562, 907, 925, 950
NlaIV GGNNCC 3 cut(s) 204, 632, 813
NmeAIII GCCGAG 2 cut(s) 82, 676
NmuCI GTSAC 1 cut(s) 917
NsbI TGCGCA 1 cut(s) 405
NspI RCATGY 2 cut(s) 562, 925
PciI ACATGT 1 cut(s) 921
PcsI WCGNNNNNNNCGW 6 cut(s) 144, 384, 393, 571, 702, 729
PctI GAATGC 1 cut(s) 835
PdiI GCCGGC 1 cut(s) 754
PdmI GAANNNNTTC 1 cut(s) 161
PfeI GAWTC 5 cut(s) 124, 170, 523, 637, 707
PflFI GACNNNGTC 1 cut(s) 570
PinAI ACCGGT 1 cut(s) 714
Ple19I CGATCG 2 cut(s) 218, 684
Ppu21I YACGTR 1 cut(s) 742
PscI ACATGT 1 cut(s) 921
Psp6I CCWGG 1 cut(s) 261
PspEI GGTNACC 1 cut(s) 950
PspGI CCWGG 1 cut(s) 261
PspN4I GGNNCC 3 cut(s) 204, 632, 813
PspPI GGNCC 2 cut(s) 648, 712
PstI CTGCAG 1 cut(s) 774
PstNI CAGNNNCTG 1 cut(s) 268
PsuI RGATCY 2 cut(s) 202, 654
PsyI GACNNNGTC 1 cut(s) 570
PvuI CGATCG 2 cut(s) 218, 684
RsaI GTAC 2 cut(s) 77, 719
RsaNI GTAC 2 cut(s) 76, 718
Rsr2I CGGWCCG 1 cut(s) 712
RsrII CGGWCCG 1 cut(s) 712
Sau3AI GATC 9 cut(s) 41, 202, 215, 223, 500, 532, 654, 681, 1030
Sau96I GGNCC 2 cut(s) 648, 712
ScaI AGTACT 1 cut(s) 77
ScrFI CCNGG 3 cut(s) 179, 263, 1001
SduI GDGCHC 1 cut(s) 814
SfaNI GCATC 3 cut(s) 292, 391, 975
SfcI CTRYAG 1 cut(s) 770
SgrAI CRCCGGYG 1 cut(s) 817
SinI GGWCC 1 cut(s) 712
Sse9I AATT 3 cut(s) 676, 686, 1108
StyD4I CCNGG 3 cut(s) 177, 261, 999
StyI CCWWGG 2 cut(s) 777, 974
TaaI ACNGT 2 cut(s) 118, 410
TaiI ACGT 2 cut(s) 582, 744
TaqII GACCGA 1 cut(s) 841
TasI AATT 3 cut(s) 676, 686, 1108
TatI WGTACW 1 cut(s) 75
TfiI GAWTC 5 cut(s) 124, 170, 523, 637, 707
TscAI CASTG 3 cut(s) 95, 203, 415
TseFI GTSAC 1 cut(s) 917
TseI GCWGC 6 cut(s) 316, 621, 749, 769, 934, 1018
Tsp45I GTSAC 1 cut(s) 917
TspDTI ATGAA 2 cut(s) 531, 564
TspGWI ACGGA 1 cut(s) 77
TspRI CASTG 3 cut(s) 95, 203, 415
Tth111I GACNNNGTC 1 cut(s) 570
VpaK11BI GGWCC 1 cut(s) 712
XapI RAATTY 1 cut(s) 686
XceI RCATGY 2 cut(s) 562, 925
XcmI CCANNNNNNNNNTGG 1 cut(s) 115
XmnI GAANNNNTTC 1 cut(s) 161
ZrmI AGTACT 1 cut(s) 77
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.