AT5G65274

Functions as component of the Arp2 3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0015980
Physical Location & Seq
Forward (+)
26085300 .. 26085960
661 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G65274.1

Sequence Viewer

Length: 408 bp
ATGGCTGGTGCAGCAGCATTTGTTGAAGCTGATAATGCAGAGGAAATAATGGCAAGGATCGAAACCAAATCGCAGAAGATTGAAAGCTTACTCAAACACTACAAACATGTCGAAGCTCTTAAAACGGCTCTTGATGGTTCGCCACCTAAAACTCTCGACGAACGTTGCAAGTCAGCTAATTGGATAGTTGTGCACAGAGCTATCATGGCGATAAAGGATATCGAAGGAATGTTAAATTCTCTTACTGCCGAGTATTACGACATTCTCATGAAGTACTTGTACAGAGGTCTTTCGACTGGTGACGAACCCACATGCGAACAGTGTTTGATGATTCACGAGAAACTAACAGAGAGAGCTGGTCTCGGTTGCATTCTCCGTTGTCTGAGTGATACTACCAATACCGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000902 GO:0000904 GO:0003674 GO:0003779 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005856 GO:0005885 GO:0006928 GO:0006996 GO:0007010 GO:0007015 GO:0007163 GO:0007275 GO:0007399 GO:0007417 GO:0007420 GO:0008064 GO:0008092 GO:0008150 GO:0008154 GO:0009653 GO:0009825 GO:0009888 GO:0009987 GO:0010026 GO:0010090 GO:0010638 GO:0012505 GO:0014812 GO:0014909 GO:0015629 GO:0016043 GO:0016049 GO:0016477 GO:0021761 GO:0021769 GO:0022607 GO:0030011 GO:0030029 GO:0030030 GO:0030036 GO:0030041 GO:0030154 GO:0030424 GO:0030426 GO:0030427 GO:0030832 GO:0030833 GO:0030838 GO:0030900 GO:0031334 GO:0031410 GO:0031982 GO:0032271 GO:0032273 GO:0032501 GO:0032502 GO:0032535 GO:0032956 GO:0032970 GO:0032989 GO:0032991 GO:0033043 GO:0033267 GO:0034314 GO:0034622 GO:0040007 GO:0040011 GO:0042995 GO:0043005 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043232 GO:0043254 GO:0043933 GO:0044085 GO:0044087 GO:0044089 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044463 GO:0044464 GO:0044877 GO:0045010 GO:0048468 GO:0048513 GO:0048518 GO:0048522 GO:0048731 GO:0048856 GO:0048869 GO:0048870 GO:0050789 GO:0050794 GO:0051015 GO:0051128 GO:0051130 GO:0051179 GO:0051258 GO:0051493 GO:0051495 GO:0051639 GO:0051674 GO:0060322 GO:0061842 GO:0065003 GO:0065007 GO:0065008 GO:0071840 GO:0090066 GO:0090558 GO:0090626 GO:0097435 GO:0097458 GO:0097581 GO:0097708 GO:0110053 GO:0120025 GO:0120036 GO:0120038 GO:0150034 GO:1902903 GO:1902905
KEGG Pathways
Metabolic & Signaling
ko04144 ko04666 ko04810 ko05100 ko05130 ko05131 ko05132 map04144 map04666 map04810 map05100 map05130 map05131 map05132
Pfam Domains
Protein Families
P16-Arc

Protein Analysis

135

Amino Acids

15.07

Weight (kDa)

5.56

Isoelectric Point (pI)

40.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
P16-Arc PF04699 10 - 131 1.4e-23 ARP2/3 complex 16 kDa subunit (p16-Arc)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015980)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G01710 AT5G65274
fragaria_vesca FvH4_7g09830
malus_domestica MD02G1216700.v1.1 MD15G1416400.v1.1
prunus_persica Prupe.2G127400_v2.0.a1
pyrus_communis pycom02g17840
rosa_chinensis RchiOBHm_Chr1g0346991
rosa_laevigata RLG00000028765
rosa_multiflora Rmu_sc0002705.1_g000019
rosa_roxburghii Rroxscaffold_4G00307990
rosa_samantha Rh1AG202900 Rh1CG187600 Rh1DG199600
rosa_wichuraiana Rw1G017000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclI AACGTT 1 cut(s) 163
AclWI GGATC 1 cut(s) 65
AcsI RAATTY 1 cut(s) 235
AfaI GTAC 2 cut(s) 275, 281
AflIII ACRYGT 1 cut(s) 106
AgsI TTSAA 2 cut(s) 26, 83
AluBI AGCT 6 cut(s) 29, 87, 116, 176, 200, 356
AluI AGCT 6 cut(s) 29, 87, 116, 176, 200, 356
Alw21I GWGCWC 1 cut(s) 195
Alw26I GTCTC 1 cut(s) 365
Alw44I GTGCAC 1 cut(s) 191
AlwI GGATC 1 cut(s) 65
ApaLI GTGCAC 1 cut(s) 191
ApeKI GCWGC 2 cut(s) 11, 14
ApoI RAATTY 1 cut(s) 235
AsuHPI GGTGA 1 cut(s) 311
BaeGI GKGCMC 1 cut(s) 195
BarI GAAGNNNNNNTAC 2 cut(s) 263, 295
BauI CACGAG 1 cut(s) 335
Bbv12I GWGCWC 1 cut(s) 195
BbvI GCAGC 2 cut(s) 23, 26
BccI CCATC 1 cut(s) 128
BceAI ACGGC 1 cut(s) 141
BcoDI GTCTC 1 cut(s) 365
BisI GCNGC 2 cut(s) 12, 15
BlsI GCNGC 2 cut(s) 13, 16
BmcAI AGTACT 1 cut(s) 275
BplI GAGNNNNNCTC 2 cut(s) 345, 377
BsaI GGTCTC 1 cut(s) 365
Bse1I ACTGG 1 cut(s) 301
BseMII CTCAG 1 cut(s) 374
BseNI ACTGG 1 cut(s) 301
BseSI GKGCMC 1 cut(s) 195
BseXI GCAGC 2 cut(s) 23, 26
BsgI GTGCAG 1 cut(s) 30
BsiHKAI GWGCWC 1 cut(s) 195
BsmAI GTCTC 1 cut(s) 365
BsmI GAATGC 1 cut(s) 369
Bso31I GGTCTC 1 cut(s) 365
Bsp1286I GDGCHC 1 cut(s) 195
Bsp1407I TGTACA 1 cut(s) 279
Bsp143I GATC 1 cut(s) 57
BspCNI CTCAG 1 cut(s) 375
BspHI TCATGA 1 cut(s) 267
BspPI GGATC 1 cut(s) 65
BspTNI GGTCTC 1 cut(s) 365
BsrGI TGTACA 1 cut(s) 279
BsrI ACTGG 1 cut(s) 301
BssMI GATC 1 cut(s) 57
BssSI CACGAG 1 cut(s) 335
Bst2BI CACGAG 1 cut(s) 335
Bst4CI ACNGT 2 cut(s) 321, 403
BstAUI TGTACA 1 cut(s) 279
BstDEI CTNAG 1 cut(s) 383
BstKTI GATC 1 cut(s) 60
BstMAI GTCTC 1 cut(s) 365
BstMBI GATC 1 cut(s) 57
BstMWI GCNNNNNNNGC 3 cut(s) 11, 35, 206
BstNSI RCATGY 2 cut(s) 110, 315
BstSLI GKGCMC 1 cut(s) 195
BstV1I GCAGC 2 cut(s) 23, 26
BtsIMutI CAGTG 1 cut(s) 326
CciI TCATGA 1 cut(s) 267
Csp6I GTAC 2 cut(s) 274, 280
CviAII CATG 4 cut(s) 107, 205, 268, 312
CviJI RGCY 8 cut(s) 5, 29, 87, 116, 128, 176, 200, 356
CviKI_1 RGCY 8 cut(s) 5, 29, 87, 116, 128, 176, 200, 356
CviQI GTAC 2 cut(s) 274, 280
DdeI CTNAG 1 cut(s) 383
DpnI GATC 1 cut(s) 59
DpnII GATC 1 cut(s) 57
Eco31I GGTCTC 1 cut(s) 365
Eco32I GATATC 1 cut(s) 220
EcoRV GATATC 1 cut(s) 220
FaeI CATG 4 cut(s) 110, 208, 271, 315
FaiI YATR 4 cut(s) 108, 206, 269, 313
FatI CATG 4 cut(s) 106, 204, 267, 311
Fnu4HI GCNGC 2 cut(s) 12, 15
Fsp4HI GCNGC 2 cut(s) 12, 15
GluI GCNGC 2 cut(s) 12, 15
Hin1II CATG 4 cut(s) 110, 208, 271, 315
HindIII AAGCTT 1 cut(s) 85
HinfI GANTC 1 cut(s) 331
HphI GGTGA 1 cut(s) 311
Hpy166II GTNNAC 1 cut(s) 193
Hpy188I TCNGA 1 cut(s) 384
Hpy188III TCNNGA 4 cut(s) 131, 155, 268, 335
Hpy8I GTNNAC 1 cut(s) 193
Hpy99I CGWCG 1 cut(s) 161
HpyAV CCTTC 1 cut(s) 218
HpyCH4III ACNGT 2 cut(s) 321, 403
HpyCH4IV ACGT 1 cut(s) 163
HpyCH4V TGCA 5 cut(s) 11, 38, 168, 193, 369
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 35, 206
HpyF3I CTNAG 1 cut(s) 383
HpySE526I ACGT 1 cut(s) 163
Hsp92II CATG 4 cut(s) 110, 208, 271, 315
Kzo9I GATC 1 cut(s) 57
LpnPI CCDG 2 cut(s) 282, 342
Lsp1109I GCAGC 2 cut(s) 23, 26
MaeII ACGT 1 cut(s) 163
MaeIII GTNAC 1 cut(s) 299
MalI GATC 1 cut(s) 59
MboI GATC 1 cut(s) 57
MboII GAAGA 1 cut(s) 88
MhlI GDGCHC 1 cut(s) 195
MluCI AATT 2 cut(s) 178, 235
MnlI CCTC 2 cut(s) 34, 278
MseI TTAA 3 cut(s) 120, 233, 406
MslI CAYNNNNRTG 1 cut(s) 266
Mva1269I GAATGC 1 cut(s) 369
MwoI GCNNNNNNNGC 3 cut(s) 11, 35, 206
NdeII GATC 1 cut(s) 57
NlaIII CATG 4 cut(s) 110, 208, 271, 315
NmeAIII GCCGAG 1 cut(s) 274
NmuCI GTSAC 1 cut(s) 299
NspI RCATGY 2 cut(s) 110, 315
PagI TCATGA 1 cut(s) 267
PciI ACATGT 1 cut(s) 106
PctI GAATGC 1 cut(s) 369
PfeI GAWTC 1 cut(s) 331
PkrI GCNGC 2 cut(s) 13, 16
PscI ACATGT 1 cut(s) 106
Psp1406I AACGTT 1 cut(s) 163
RsaI GTAC 2 cut(s) 275, 281
RsaNI GTAC 2 cut(s) 274, 280
RseI CAYNNNNRTG 1 cut(s) 266
SaqAI TTAA 3 cut(s) 120, 233, 406
SatI GCNGC 2 cut(s) 12, 15
Sau3AI GATC 1 cut(s) 57
ScaI AGTACT 1 cut(s) 275
SduI GDGCHC 1 cut(s) 195
SetI ASST 9 cut(s) 31, 89, 118, 148, 166, 178, 202, 289, 358
SmiMI CAYNNNNRTG 1 cut(s) 266
Sse9I AATT 2 cut(s) 178, 235
TaaI ACNGT 2 cut(s) 321, 403
TaiI ACGT 1 cut(s) 166
TaqI TCGA 5 cut(s) 60, 111, 156, 222, 293
TasI AATT 2 cut(s) 178, 235
TatI WGTACW 2 cut(s) 273, 279
TfiI GAWTC 1 cut(s) 331
Tru1I TTAA 3 cut(s) 120, 233, 406
Tru9I TTAA 3 cut(s) 120, 233, 406
TscAI CASTG 1 cut(s) 326
TseFI GTSAC 1 cut(s) 299
TseI GCWGC 2 cut(s) 11, 14
Tsp45I GTSAC 1 cut(s) 299
TspDTI ATGAA 1 cut(s) 284
TspGWI ACGGA 1 cut(s) 365
TspRI CASTG 1 cut(s) 326
VneI GTGCAC 1 cut(s) 191
XapI RAATTY 1 cut(s) 235
XceI RCATGY 2 cut(s) 110, 315
ZrmI AGTACT 1 cut(s) 275
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.