AT5G65340

Protein of unknown function, DUF617

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0015260
Physical Location & Seq
Reverse (-)
26113349 .. 26114545
1197 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G65340.1

Sequence Viewer

Length: 762 bp
ATGTCCGCCTTCCCCACAAACCCTTACCGTCGCCGCCTAGCTTCAACCACCACAAGCGCAACGGTAGCCGTAGACTGCCATAAACAAGTTCGTTCATGGCGGCTCCTTCGCACCATCGCCCAACTTCTCATCCCATCTTGTTACTGCACAATCGTCGATCCAAATGATAGTCAAGAAGACAAATTATCTCACCGCCAAATTAAACCAAGAACCTCTTCCGCCTCCTCAACCGCCACAAACTCCACTTTTACCGGAACCATATTCGGTTTCCGTCGTGGCAAAGTAAACTTCTGCATCCAAGCAACAAACTCCAAAACCCTAAACCCAATCATTGTCCTCTTAGAGCTCACTGTCCCTACCGAGGTATTAGCCCGCGAAATGCAAGGAGGCGTGCTCAGGATCGCTCTCGAATCAAACAATAACGACGGATATGATTCACATGAAGATTCTTCCTCTTCACTTCTTACAACACCTTTATGGAACATGTATTGCAACGGTCGGAAAGTTGGATTCGCCATCAAACGAGAGCCTTCGAAGTCTGAGCTAGCTGCGTTGAAGGTGCTTACTCCGGTGGCTGAAGGAGCCGGAGTTGTTAACGGAGAAGAGATAAACCGAGAGAAAAGTGATCATATGATGTACTTAAGAGCGAGTTTCAAACGAGTTTTTGGTTCGTTTGATTCGGAATCTTTCCATCTCGTTGACCCACGTGGGATTATCGGTCAAGAACTAAGTATCTTCTTTTCTAGATCGTCAAAAAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF617

Protein Analysis

253

Amino Acids

28.13

Weight (kDa)

9.4

Isoelectric Point (pI)

47.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF617 PF04759 84 - 249 2.6e-60 Protein of unknown function, DUF617
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015260)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G22460 AT5G65340
fragaria_vesca FvH4_1g20130
malus_domestica MD15G1317100.v1.1
prunus_persica Prupe.6G194800_v2.0.a1
pyrus_communis pycom15g27970
rosa_chinensis RchiOBHm_Chr2g0112181
rosa_laevigata RLG00000017956
rosa_multiflora Rmu_sc0000360.1_g000039
rosa_roxburghii Rroxscaffold_2G00131710
rosa_rugosa Rorug02G0178900
rosa_samantha Rh2AG231100 Rh2BG244100 Rh2CG235500 Rh2DG238900
rosa_wichuraiana Rw2G017910

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 72
AccII CGCG 1 cut(s) 375
AciI CCGC 7 cut(s) 6, 34, 100, 193, 219, 231, 373
AclWI GGATC 2 cut(s) 152, 407
AcuI CTGAAG 1 cut(s) 597
AcvI CACGTG 1 cut(s) 707
AfaI GTAC 1 cut(s) 638
AfiI CCNNNNNNNGG 1 cut(s) 361
AflII CTTAAG 1 cut(s) 640
AflIII ACRYGT 1 cut(s) 483
AgsI TTSAA 3 cut(s) 45, 556, 655
AjuI GAANNNNNNNTTGG 2 cut(s) 199, 231
AluBI AGCT 4 cut(s) 41, 346, 544, 548
AluI AGCT 4 cut(s) 41, 346, 544, 548
Alw21I GWGCWC 2 cut(s) 348, 396
AlwI GGATC 2 cut(s) 152, 407
ApeKI GCWGC 1 cut(s) 548
Asp700I GAANNNNTTC 1 cut(s) 214
AspLEI GCGC 1 cut(s) 59
AsuHPI GGTGA 1 cut(s) 182
AsuII TTCGAA 1 cut(s) 533
AsuNHI GCTAGC 1 cut(s) 544
BanII GRGCYC 1 cut(s) 348
BbrPI CACGTG 1 cut(s) 707
BbsI GAAGAC 1 cut(s) 183
Bbv12I GWGCWC 2 cut(s) 348, 396
BbvI GCAGC 1 cut(s) 535
BccI CCATC 4 cut(s) 122, 142, 524, 699
BceAI ACGGC 1 cut(s) 53
BcgI CGANNNNNNTGC 2 cut(s) 136, 170
BclI TGATCA 1 cut(s) 625
BfaI CTAG 3 cut(s) 38, 545, 744
BfrI CTTAAG 1 cut(s) 640
BisI GCNGC 3 cut(s) 34, 101, 549
BlsI GCNGC 3 cut(s) 35, 102, 550
BmiI GGNNCC 3 cut(s) 104, 256, 583
BmsI GCATC 1 cut(s) 303
BmtI GCTAGC 1 cut(s) 548
BpiI GAAGAC 1 cut(s) 183
BplI GAGNNNNNCTC 2 cut(s) 378, 410
Bpu10I CCTNAGC 1 cut(s) 395
Bpu14I TTCGAA 1 cut(s) 533
BsaAI YACGTR 1 cut(s) 707
BsaJI CCNNGG 1 cut(s) 360
BsaWI WCCGGW 2 cut(s) 251, 568
BsaXI ACNNNNNCTCC 2 cut(s) 573, 603
Bsc4I CCNNNNNNNGG 1 cut(s) 361
BseDI CCNNGG 1 cut(s) 360
BseGI GGATG 2 cut(s) 129, 294
BseLI CCNNNNNNNGG 1 cut(s) 361
BseMII CTCAG 2 cut(s) 409, 531
BseRI GAGGAG 1 cut(s) 214
BseXI GCAGC 1 cut(s) 535
BsgI GTGCAG 1 cut(s) 130
Bsh1236I CGCG 1 cut(s) 375
Bsh1285I CGRYCG 1 cut(s) 499
BsiEI CGRYCG 1 cut(s) 499
BsiHKAI GWGCWC 2 cut(s) 348, 396
BsiSI CCGG 3 cut(s) 252, 569, 585
BslFI GGGAC 1 cut(s) 338
BslI CCNNNNNNNGG 1 cut(s) 361
BsmFI GGGAC 1 cut(s) 338
Bsp119I TTCGAA 1 cut(s) 533
Bsp1286I GDGCHC 2 cut(s) 348, 396
Bsp143I GATC 4 cut(s) 157, 399, 625, 746
BspACI CCGC 7 cut(s) 6, 34, 100, 193, 219, 231, 373
BspCNI CTCAG 2 cut(s) 408, 532
BspFNI CGCG 1 cut(s) 375
BspLI GGNNCC 3 cut(s) 104, 256, 583
BspOI GCTAGC 1 cut(s) 548
BspPI GGATC 2 cut(s) 152, 407
BspT104I TTCGAA 1 cut(s) 533
BspTI CTTAAG 1 cut(s) 640
BssECI CCNNGG 1 cut(s) 360
BssMI GATC 4 cut(s) 157, 399, 625, 746
Bst4CI ACNGT 4 cut(s) 29, 64, 352, 497
Bst6I CTCTTC 3 cut(s) 220, 460, 597
BstAFI CTTAAG 1 cut(s) 640
BstBAI YACGTR 1 cut(s) 707
BstBI TTCGAA 1 cut(s) 533
BstC8I GCNNGC 3 cut(s) 373, 392, 546
BstDEI CTNAG 4 cut(s) 340, 395, 540, 728
BstF5I GGATG 2 cut(s) 129, 294
BstFNI CGCG 1 cut(s) 375
BstHHI GCGC 1 cut(s) 59
BstKTI GATC 4 cut(s) 160, 402, 628, 749
BstMBI GATC 4 cut(s) 157, 399, 625, 746
BstMCI CGRYCG 1 cut(s) 499
BstMWI GCNNNNNNNGC 2 cut(s) 65, 581
BstNSI RCATGY 1 cut(s) 487
BstUI CGCG 1 cut(s) 375
BstV1I GCAGC 1 cut(s) 535
BstV2I GAAGAC 1 cut(s) 183
BtgZI GCGATG 1 cut(s) 100
BtsCI GGATG 2 cut(s) 129, 294
BtsIMutI CAGTG 1 cut(s) 348
Cac8I GCNNGC 3 cut(s) 373, 392, 546
CfoI GCGC 1 cut(s) 59
Csp6I GTAC 1 cut(s) 637
CviAII CATG 3 cut(s) 96, 440, 484
CviQI GTAC 1 cut(s) 637
DdeI CTNAG 4 cut(s) 340, 395, 540, 728
DpnI GATC 4 cut(s) 159, 401, 627, 748
DpnII GATC 4 cut(s) 157, 399, 625, 746
Eam1104I CTCTTC 3 cut(s) 220, 460, 597
EarI CTCTTC 3 cut(s) 220, 460, 597
EciI GGCGGA 1 cut(s) 208
Ecl136II GAGCTC 1 cut(s) 346
Eco24I GRGCYC 1 cut(s) 348
Eco53kI GAGCTC 1 cut(s) 346
Eco57I CTGAAG 1 cut(s) 597
Eco72I CACGTG 1 cut(s) 707
EcoICRI GAGCTC 1 cut(s) 346
EcoT38I GRGCYC 1 cut(s) 348
FaeI CATG 3 cut(s) 99, 443, 487
FaiI YATR 9 cut(s) 81, 97, 260, 432, 441, 478, 485, 630, 632
FalI AAGNNNNNCTT 2 cut(s) 199, 231
FaqI GGGAC 1 cut(s) 338
FatI CATG 3 cut(s) 95, 439, 483
FauI CCCGC 1 cut(s) 380
FauNDI CATATG 1 cut(s) 630
FbaI TGATCA 1 cut(s) 625
FblI GTMKAC 1 cut(s) 72
Fnu4HI GCNGC 3 cut(s) 34, 101, 549
FokI GGATG 2 cut(s) 116, 281
FriOI GRGCYC 1 cut(s) 348
Fsp4HI GCNGC 3 cut(s) 34, 101, 549
FspBI CTAG 3 cut(s) 38, 545, 744
GlaI GCGC 1 cut(s) 58
GluI GCNGC 3 cut(s) 34, 101, 549
HapII CCGG 3 cut(s) 252, 569, 585
HhaI GCGC 1 cut(s) 59
Hin1II CATG 3 cut(s) 99, 443, 487
Hin6I GCGC 1 cut(s) 57
HinP1I GCGC 1 cut(s) 57
HincII GTYRAC 2 cut(s) 595, 700
HindII GTYRAC 2 cut(s) 595, 700
HinfI GANTC 6 cut(s) 410, 434, 446, 510, 677, 683
HpaI GTTAAC 1 cut(s) 595
HpaII CCGG 3 cut(s) 252, 569, 585
HphI GGTGA 1 cut(s) 182
Hpy166II GTNNAC 4 cut(s) 73, 286, 595, 700
Hpy188I TCNGA 3 cut(s) 501, 541, 682
Hpy188III TCNNGA 5 cut(s) 173, 397, 407, 722, 744
Hpy8I GTNNAC 4 cut(s) 73, 286, 595, 700
Hpy99I CGWCG 4 cut(s) 33, 158, 276, 428
HpyAV CCTTC 5 cut(s) 19, 116, 540, 550, 572
HpyCH4III ACNGT 4 cut(s) 29, 64, 352, 497
HpyCH4IV ACGT 1 cut(s) 706
HpyCH4V TGCA 4 cut(s) 147, 294, 382, 492
HpyF10VI GCNNNNNNNGC 2 cut(s) 65, 581
HpyF3I CTNAG 4 cut(s) 340, 395, 540, 728
HpySE526I ACGT 1 cut(s) 706
Hsp92II CATG 3 cut(s) 99, 443, 487
HspAI GCGC 1 cut(s) 57
Ksp22I TGATCA 1 cut(s) 625
KspAI GTTAAC 1 cut(s) 595
Kzo9I GATC 4 cut(s) 157, 399, 625, 746
LmnI GCTCC 2 cut(s) 108, 581
LpnPI CCDG 4 cut(s) 265, 382, 582, 598
Lsp1109I GCAGC 1 cut(s) 535
LweI GCATC 1 cut(s) 303
MaeI CTAG 3 cut(s) 38, 545, 744
MaeII ACGT 1 cut(s) 706
MaeIII GTNAC 1 cut(s) 140
MalI GATC 4 cut(s) 159, 401, 627, 748
MboI GATC 4 cut(s) 157, 399, 625, 746
MboII GAAGA 7 cut(s) 188, 207, 441, 447, 455, 614, 727
MhlI GDGCHC 2 cut(s) 348, 396
MluCI AATT 2 cut(s) 182, 198
MmeI TCCRAC 2 cut(s) 479, 487
MnlI CCTC 7 cut(s) 223, 232, 235, 347, 355, 380, 463
MroXI GAANNNNTTC 1 cut(s) 214
MseI TTAA 3 cut(s) 201, 594, 641
MslI CAYNNNNRTG 1 cut(s) 475
MspCI CTTAAG 1 cut(s) 640
MspI CCGG 3 cut(s) 252, 569, 585
MvnI CGCG 1 cut(s) 375
MwoI GCNNNNNNNGC 2 cut(s) 65, 581
NdeI CATATG 1 cut(s) 630
NdeII GATC 4 cut(s) 157, 399, 625, 746
NheI GCTAGC 1 cut(s) 544
NlaIII CATG 3 cut(s) 99, 443, 487
NlaIV GGNNCC 3 cut(s) 104, 256, 583
NspI RCATGY 1 cut(s) 487
NspV TTCGAA 1 cut(s) 533
PciI ACATGT 1 cut(s) 483
PdmI GAANNNNTTC 1 cut(s) 214
PfeI GAWTC 6 cut(s) 410, 434, 446, 510, 677, 683
PkrI GCNGC 3 cut(s) 35, 102, 550
PmaCI CACGTG 1 cut(s) 707
PmlI CACGTG 1 cut(s) 707
Ppu21I YACGTR 1 cut(s) 707
PscI ACATGT 1 cut(s) 483
Psp124BI GAGCTC 1 cut(s) 348
PspCI CACGTG 1 cut(s) 707
PspN4I GGNNCC 3 cut(s) 104, 256, 583
RsaI GTAC 1 cut(s) 638
RsaNI GTAC 1 cut(s) 637
RseI CAYNNNNRTG 1 cut(s) 475
SacI GAGCTC 1 cut(s) 348
SaqAI TTAA 3 cut(s) 201, 594, 641
SatI GCNGC 3 cut(s) 34, 101, 549
Sau3AI GATC 4 cut(s) 157, 399, 625, 746
SduI GDGCHC 2 cut(s) 348, 396
SetI ASST 9 cut(s) 43, 215, 348, 366, 475, 546, 550, 561, 709
SfaNI GCATC 1 cut(s) 303
SfuI TTCGAA 1 cut(s) 533
SmiMI CAYNNNNRTG 1 cut(s) 475
SmlI CTYRAG 1 cut(s) 640
SmoI CTYRAG 1 cut(s) 640
Sse9I AATT 2 cut(s) 182, 198
SsiI CCGC 7 cut(s) 6, 34, 100, 193, 219, 231, 373
SspMI CTAG 3 cut(s) 38, 545, 744
SstI GAGCTC 1 cut(s) 348
TaaI ACNGT 4 cut(s) 29, 64, 352, 497
TaiI ACGT 1 cut(s) 709
TaqI TCGA 3 cut(s) 156, 408, 533
TaqII GACCGA 1 cut(s) 707
TasI AATT 2 cut(s) 182, 198
TatI WGTACW 1 cut(s) 636
TauI GCSGC 2 cut(s) 36, 103
TfiI GAWTC 6 cut(s) 410, 434, 446, 510, 677, 683
Tru1I TTAA 3 cut(s) 201, 594, 641
Tru9I TTAA 3 cut(s) 201, 594, 641
TscAI CASTG 1 cut(s) 355
TseI GCWGC 1 cut(s) 548
TspDTI ATGAA 2 cut(s) 84, 456
TspGWI ACGGA 3 cut(s) 260, 441, 612
TspRI CASTG 1 cut(s) 355
Vha464I CTTAAG 1 cut(s) 640
XbaI TCTAGA 1 cut(s) 743
XceI RCATGY 1 cut(s) 487
XmiI GTMKAC 1 cut(s) 72
XmnI GAANNNNTTC 1 cut(s) 214
XspI CTAG 3 cut(s) 38, 545, 744
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.