MD15G1317100.v1.1

Protein of unknown function, DUF617

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0015260
Physical Location & Seq
Forward (+)
32700193 .. 32700981
789 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1317100.v1.1.491

Sequence Viewer

Length: 729 bp
ATGGCCTACCCTAGAGTTGGTGCCACCACCATAACCACCGGCGTCACCACCGTTGACTGCCAAAAACAAGTCCGGTCATGGCGGCTTCTTCGCTCTCTCATCGAAGTTCTAATCCCAACTTGCAATTGCACCTTCGTAGAAGATGATCATCATCAAGAAAAACTAAACACCAAACAACAAAGCCACGTCAACAGATACTACTATCCCCAGCCAAGCTTTGCTTGCTCCAACAACATCATAATCGGCACCATATTCGGATACCGAAGAGGGAAAGTTAGTTTCTGTATCCAAACAACCTCCCAGTCCAACCCAATTCTCCTTCTCGAGCTAGCCTTGCCCACAACCGTTCTAGCCAAAGAAATGCAAGGTGGGTTTCTTAGAATAGCGCTCGAGGCAGCTACTGATCCAAATAGTTCTTCTAACTCTCTTTTGGCCACGCCTGTATGGACCATGTATTGCAATAGTAAAAAAGTTGGGTATGCAATCAAGCGCAAGCCTTCAAAGCATGACATGGAAGCACTGAGGGTGATGGGATCAGTTCTGGTGGGTGCTGGGATTATTAATGGGAATAAAGAGCTGAATAATTATAGGGAGGGTGATGAGATTATGTACCTGAGAGCCAATTTTCAGCGAGTTTGTGGTTCAGCTAATTCTGAGTCCTTTCATTTGATTGATCCAGAGGAGAGTATCGGCCAGGAGCTTAGTATTTTCCTTTATCGGTCAAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF617

Protein Analysis

243

Amino Acids

27.08

Weight (kDa)

8.64

Isoelectric Point (pI)

45.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF617 PF04759 80 - 240 8.7e-64 Protein of unknown function, DUF617
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015260)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G22460 AT5G65340
fragaria_vesca FvH4_1g20130
malus_domestica MD15G1317100.v1.1
prunus_persica Prupe.6G194800_v2.0.a1
pyrus_communis pycom15g27970
rosa_chinensis RchiOBHm_Chr2g0112181
rosa_laevigata RLG00000017956
rosa_multiflora Rmu_sc0000360.1_g000039
rosa_roxburghii Rroxscaffold_2G00131710
rosa_rugosa Rorug02G0178900
rosa_samantha Rh2AG231100 Rh2BG244100 Rh2CG235500 Rh2DG238900
rosa_wichuraiana Rw2G017910

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 20, 245
AciI CCGC 1 cut(s) 82
AclWI GGATC 3 cut(s) 398, 541, 668
AcoI YGGCCR 2 cut(s) 432, 691
AcyI GRCGYC 1 cut(s) 42
AfaI GTAC 1 cut(s) 611
AfeI AGCGCT 1 cut(s) 387
AfiI CCNNNNNNNGG 1 cut(s) 17
AgsI TTSAA 1 cut(s) 501
AjiI CACGTC 1 cut(s) 187
AjnI CCWGG 1 cut(s) 693
AjuI GAANNNNNNNTTGG 2 cut(s) 400, 432
AluBI AGCT 7 cut(s) 216, 328, 398, 577, 647, 700, 726
AluI AGCT 7 cut(s) 216, 328, 398, 577, 647, 700, 726
AlwI GGATC 3 cut(s) 398, 541, 668
AlwNI CAGNNNCTG 1 cut(s) 401
Ama87I CYCGRG 2 cut(s) 323, 389
Aor51HI AGCGCT 1 cut(s) 387
AoxI GGCC 3 cut(s) 3, 432, 691
ApeKI GCWGC 1 cut(s) 395
AseI ATTAAT 1 cut(s) 561
AspLEI GCGC 2 cut(s) 388, 492
AspS9I GGNCC 1 cut(s) 447
AsuHPI GGTGA 3 cut(s) 37, 538, 608
AsuNHI GCTAGC 1 cut(s) 328
AvaI CYCGRG 2 cut(s) 323, 389
AvaII GGWCC 1 cut(s) 447
BalI TGGCCA 1 cut(s) 434
BanI GGYRCC 2 cut(s) 20, 245
BbvI GCAGC 1 cut(s) 407
BccI CCATC 1 cut(s) 523
BcgI CGANNNNNNTGC 2 cut(s) 235, 269
BciT130I CCWGG 1 cut(s) 695
BciVI GTATCC 2 cut(s) 251, 296
BclI TGATCA 1 cut(s) 145
BfaI CTAG 4 cut(s) 12, 329, 350, 727
BfoI RGCGCY 1 cut(s) 389
BfuI GTATCC 2 cut(s) 251, 296
BisI GCNGC 2 cut(s) 83, 396
BlsI GCNGC 2 cut(s) 84, 397
Bme1390I CCNGG 1 cut(s) 695
Bme18I GGWCC 1 cut(s) 447
BmeT110I CYCGRG 2 cut(s) 323, 389
BmgBI CACGTC 1 cut(s) 187
BmgT120I GGNCC 1 cut(s) 447
BmiI GGNNCC 2 cut(s) 22, 247
BmrFI CCNGG 1 cut(s) 695
BmrI ACTGGG 1 cut(s) 295
BmtI GCTAGC 1 cut(s) 332
BmuI ACTGGG 1 cut(s) 295
BsaBI GATNNNNATC 2 cut(s) 147, 150
BsaHI GRCGYC 1 cut(s) 42
BsaWI WCCGGW 1 cut(s) 72
Bsc4I CCNNNNNNNGG 1 cut(s) 17
Bse118I RCCGGY 1 cut(s) 38
Bse1I ACTGG 1 cut(s) 301
Bse8I GATNNNNATC 2 cut(s) 147, 150
BseBI CCWGG 1 cut(s) 695
BseJI GATNNNNATC 2 cut(s) 147, 150
BseLI CCNNNNNNNGG 1 cut(s) 17
BseMII CTCAG 3 cut(s) 512, 605, 645
BseNI ACTGG 1 cut(s) 301
BseRI GAGGAG 1 cut(s) 695
BseXI GCAGC 1 cut(s) 407
BseYI CCCAGC 2 cut(s) 207, 551
BshFI GGCC 3 cut(s) 5, 434, 693
BshNI GGYRCC 2 cut(s) 20, 245
BsiHKCI CYCGRG 2 cut(s) 323, 389
BsiSI CCGG 2 cut(s) 39, 73
BslI CCNNNNNNNGG 1 cut(s) 17
BsnI GGCC 3 cut(s) 5, 434, 693
BsoBI CYCGRG 2 cut(s) 323, 389
Bsp143I GATC 4 cut(s) 145, 403, 533, 673
BspACI CCGC 1 cut(s) 82
BspANI GGCC 3 cut(s) 5, 434, 693
BspCNI CTCAG 3 cut(s) 513, 606, 646
BspLI GGNNCC 2 cut(s) 22, 247
BspOI GCTAGC 1 cut(s) 332
BspPI GGATC 3 cut(s) 398, 541, 668
BspT107I GGYRCC 2 cut(s) 20, 245
BsrFI RCCGGY 1 cut(s) 38
BsrI ACTGG 1 cut(s) 301
BssAI RCCGGY 1 cut(s) 38
BssMI GATC 4 cut(s) 145, 403, 533, 673
BssNI GRCGYC 1 cut(s) 42
Bst2UI CCWGG 1 cut(s) 695
Bst4CI ACNGT 2 cut(s) 52, 346
Bst6I CTCTTC 1 cut(s) 259
BstACI GRCGYC 1 cut(s) 42
BstC8I GCNNGC 3 cut(s) 223, 330, 494
BstDEI CTNAG 5 cut(s) 377, 521, 614, 654, 701
BstH2I RGCGCY 1 cut(s) 389
BstHHI GCGC 2 cut(s) 388, 492
BstKTI GATC 4 cut(s) 148, 406, 536, 676
BstMBI GATC 4 cut(s) 145, 403, 533, 673
BstMWI GCNNNNNNNGC 4 cut(s) 222, 334, 392, 502
BstNI CCWGG 1 cut(s) 695
BstSCI CCNGG 1 cut(s) 693
BstV1I GCAGC 1 cut(s) 407
BsuI GTATCC 2 cut(s) 251, 296
BsuRI GGCC 3 cut(s) 5, 434, 693
BtrI CACGTC 1 cut(s) 187
BtsIMutI CAGTG 1 cut(s) 518
Cac8I GCNNGC 3 cut(s) 223, 330, 494
CaiI CAGNNNCTG 1 cut(s) 401
CfoI GCGC 2 cut(s) 388, 492
Cfr10I RCCGGY 1 cut(s) 38
Cfr13I GGNCC 1 cut(s) 447
CseI GACGC 1 cut(s) 31
Csp6I GTAC 1 cut(s) 610
CviAII CATG 4 cut(s) 78, 451, 506, 511
CviQI GTAC 1 cut(s) 610
DdeI CTNAG 5 cut(s) 377, 521, 614, 654, 701
DpnI GATC 4 cut(s) 147, 405, 535, 675
DpnII GATC 4 cut(s) 145, 403, 533, 673
EaeI YGGCCR 2 cut(s) 432, 691
Eam1104I CTCTTC 1 cut(s) 259
EarI CTCTTC 1 cut(s) 259
Eco47I GGWCC 1 cut(s) 447
Eco47III AGCGCT 1 cut(s) 387
Eco88I CYCGRG 2 cut(s) 323, 389
EcoRII CCWGG 1 cut(s) 693
FaeI CATG 4 cut(s) 81, 454, 509, 514
FalI AAGNNNNNCTT 2 cut(s) 205, 237
FatI CATG 4 cut(s) 77, 450, 505, 510
FbaI TGATCA 1 cut(s) 145
Fnu4HI GCNGC 2 cut(s) 83, 396
Fsp4HI GCNGC 2 cut(s) 83, 396
FspBI CTAG 4 cut(s) 12, 329, 350, 727
GlaI GCGC 2 cut(s) 387, 491
GluI GCNGC 2 cut(s) 83, 396
GsaI CCCAGC 2 cut(s) 211, 555
HaeII RGCGCY 1 cut(s) 389
HaeIII GGCC 3 cut(s) 5, 434, 693
HapII CCGG 2 cut(s) 39, 73
HgaI GACGC 1 cut(s) 31
HhaI GCGC 2 cut(s) 388, 492
Hin1I GRCGYC 1 cut(s) 42
Hin1II CATG 4 cut(s) 81, 454, 509, 514
Hin6I GCGC 2 cut(s) 386, 490
HinP1I GCGC 2 cut(s) 386, 490
HincII GTYRAC 2 cut(s) 55, 190
HindII GTYRAC 2 cut(s) 55, 190
HindIII AAGCTT 1 cut(s) 214
HinfI GANTC 1 cut(s) 656
HpaII CCGG 2 cut(s) 39, 73
HphI GGTGA 3 cut(s) 37, 538, 608
Hpy166II GTNNAC 2 cut(s) 55, 190
Hpy188I TCNGA 2 cut(s) 257, 655
Hpy188III TCNNGA 3 cut(s) 155, 323, 677
Hpy8I GTNNAC 2 cut(s) 55, 190
HpyAV CCTTC 3 cut(s) 142, 329, 507
HpyCH4III ACNGT 2 cut(s) 52, 346
HpyCH4IV ACGT 1 cut(s) 186
HpyCH4V TGCA 5 cut(s) 123, 129, 364, 459, 482
HpyF10VI GCNNNNNNNGC 4 cut(s) 222, 334, 392, 502
HpyF3I CTNAG 5 cut(s) 377, 521, 614, 654, 701
HpySE526I ACGT 1 cut(s) 186
Hsp92I GRCGYC 1 cut(s) 42
Hsp92II CATG 4 cut(s) 81, 454, 509, 514
HspAI GCGC 2 cut(s) 386, 490
Ksp22I TGATCA 1 cut(s) 145
Kzo9I GATC 4 cut(s) 145, 403, 533, 673
LmnI GCTCC 2 cut(s) 230, 697
Lsp1109I GCAGC 1 cut(s) 407
MaeI CTAG 4 cut(s) 12, 329, 350, 727
MaeII ACGT 1 cut(s) 186
MaeIII GTNAC 1 cut(s) 43
MalI GATC 4 cut(s) 147, 405, 535, 675
MboI GATC 4 cut(s) 145, 403, 533, 673
MboII GAAGA 4 cut(s) 80, 152, 276, 408
MfeI CAATTG 1 cut(s) 124
MlsI TGGCCA 1 cut(s) 434
MluCI AATT 5 cut(s) 124, 312, 583, 622, 649
MluNI TGGCCA 1 cut(s) 434
MlyI GAGTC 1 cut(s) 665
MmeI TCCRAC 2 cut(s) 252, 330
MnlI CCTC 6 cut(s) 260, 307, 385, 516, 586, 673
Mox20I TGGCCA 1 cut(s) 434
MscI TGGCCA 1 cut(s) 434
MseI TTAA 1 cut(s) 561
Msp20I TGGCCA 1 cut(s) 434
MspI CCGG 2 cut(s) 39, 73
MspR9I CCNGG 1 cut(s) 695
MunI CAATTG 1 cut(s) 124
MvaI CCWGG 1 cut(s) 695
MwoI GCNNNNNNNGC 4 cut(s) 222, 334, 392, 502
NdeII GATC 4 cut(s) 145, 403, 533, 673
NheI GCTAGC 1 cut(s) 328
NlaIII CATG 4 cut(s) 81, 454, 509, 514
NlaIV GGNNCC 2 cut(s) 22, 247
NmuCI GTSAC 1 cut(s) 43
PaeR7I CTCGAG 2 cut(s) 323, 389
PkrI GCNGC 2 cut(s) 84, 397
PleI GAGTC 1 cut(s) 664
PpsI GAGTC 1 cut(s) 664
PshBI ATTAAT 1 cut(s) 561
Psp6I CCWGG 1 cut(s) 693
PspFI CCCAGC 2 cut(s) 207, 551
PspGI CCWGG 1 cut(s) 693
PspN4I GGNNCC 2 cut(s) 22, 247
PspPI GGNCC 1 cut(s) 447
PspXI VCTCGAGB 1 cut(s) 389
PstNI CAGNNNCTG 1 cut(s) 401
RsaI GTAC 1 cut(s) 611
RsaNI GTAC 1 cut(s) 610
SaqAI TTAA 1 cut(s) 561
SatI GCNGC 2 cut(s) 83, 396
Sau3AI GATC 4 cut(s) 145, 403, 533, 673
Sau96I GGNCC 1 cut(s) 447
SchI GAGTC 1 cut(s) 665
ScrFI CCNGG 1 cut(s) 695
Sfr274I CTCGAG 2 cut(s) 323, 389
SgrAI CRCCGGYG 1 cut(s) 38
SinI GGWCC 1 cut(s) 447
SlaI CTCGAG 2 cut(s) 323, 389
SmlI CTYRAG 2 cut(s) 323, 389
SmoI CTYRAG 2 cut(s) 323, 389
Sse9I AATT 5 cut(s) 124, 312, 583, 622, 649
SsiI CCGC 1 cut(s) 82
SspMI CTAG 4 cut(s) 12, 329, 350, 727
StyD4I CCNGG 1 cut(s) 693
TaaI ACNGT 2 cut(s) 52, 346
TaiI ACGT 1 cut(s) 189
TaqI TCGA 3 cut(s) 102, 324, 390
TaqII GACCGA 1 cut(s) 708
TasI AATT 5 cut(s) 124, 312, 583, 622, 649
TauI GCSGC 1 cut(s) 85
Tru1I TTAA 1 cut(s) 561
Tru9I TTAA 1 cut(s) 561
TscAI CASTG 1 cut(s) 525
TseFI GTSAC 1 cut(s) 43
TseI GCWGC 1 cut(s) 395
Tsp45I GTSAC 1 cut(s) 43
TspDTI ATGAA 1 cut(s) 653
TspRI CASTG 1 cut(s) 525
VpaK11BI GGWCC 1 cut(s) 447
VspI ATTAAT 1 cut(s) 561
XhoI CTCGAG 2 cut(s) 323, 389
XspI CTAG 4 cut(s) 12, 329, 350, 727
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.