pycom15g27970

Protein of unknown function, DUF617

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0015260
Physical Location & Seq
Forward (+)
23617847 .. 23618585
739 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g27970.1

Sequence Viewer

Length: 651 bp
ATGGCCTACCCTAGAGTTGGTGCCACCACCATAACCACCGGCGTCACCACCGTTGACTGCCAAAAACAAGTCCGGTCATGGCGGCTTCTTCGCTCTCTCATCGAAGTTCTAATCCCAACTTGCAATTGCACCTTCGTAGAAGATGATCATCATCAAGAAAATCTAGACACCAAACAACAAAGCCACGTCAACAGATACTACTGTCCCCAGCCAAGCTTTGCTTGTTCCAACAACATCATAATCGGCACCATATTCGGATACCGAAGAGGGAAAGTCAGTTTCTGTATCCAAACAACCTCCCAGTCCAACCCAATTCTCCTTCTCGAGCTAGCCTTGCCCACAACCGTTCTAGCCAAAGAAATGCAAGGTGGGTTTCTTAGAATAGCGCTCGAGGCAGCTACTGATCCAAATAGTTCTTCTAACTCTCTTTTGGCCACGCCTGTATGGACCATGTATTGCAATAGTAAAAAAGTTGGGTATGCAATCAAGCGCAAGCCTTCAAAGCATGACATGGAAGGTGATGAGATTATGTACCTGAGAGCCAATTTTCAGCGAGTTTGTGGTTCAGCTAATTCTGAGTCCTTTCATTTGATTGATCCAGAGGAGAGTATCGGCCAGGAGCTCAGAATTTTCCTTTATCGGTCAAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF617

Protein Analysis

217

Amino Acids

24.38

Weight (kDa)

8.41

Isoelectric Point (pI)

51.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF617 PF04759 80 - 171 1.6e-34 Protein of unknown function, DUF617
DUF617 PF04759 171 - 214 5.9e-15 Protein of unknown function, DUF617
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015260)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G22460 AT5G65340
fragaria_vesca FvH4_1g20130
malus_domestica MD15G1317100.v1.1
prunus_persica Prupe.6G194800_v2.0.a1
pyrus_communis pycom15g27970
rosa_chinensis RchiOBHm_Chr2g0112181
rosa_laevigata RLG00000017956
rosa_multiflora Rmu_sc0000360.1_g000039
rosa_roxburghii Rroxscaffold_2G00131710
rosa_rugosa Rorug02G0178900
rosa_samantha Rh2AG231100 Rh2BG244100 Rh2CG235500 Rh2DG238900
rosa_wichuraiana Rw2G017910

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 20, 245
AciI CCGC 1 cut(s) 82
AclWI GGATC 2 cut(s) 398, 590
AcoI YGGCCR 2 cut(s) 432, 613
AcsI RAATTY 1 cut(s) 627
AcyI GRCGYC 1 cut(s) 42
AfaI GTAC 1 cut(s) 533
AfeI AGCGCT 1 cut(s) 387
AfiI CCNNNNNNNGG 1 cut(s) 17
AgsI TTSAA 1 cut(s) 501
AjiI CACGTC 1 cut(s) 187
AjnI CCWGG 1 cut(s) 615
AjuI GAANNNNNNNTTGG 2 cut(s) 400, 432
AluBI AGCT 5 cut(s) 216, 328, 398, 569, 622
AluI AGCT 5 cut(s) 216, 328, 398, 569, 622
Alw21I GWGCWC 1 cut(s) 624
AlwI GGATC 2 cut(s) 398, 590
AlwNI CAGNNNCTG 2 cut(s) 282, 401
Ama87I CYCGRG 2 cut(s) 323, 389
Aor51HI AGCGCT 1 cut(s) 387
AoxI GGCC 3 cut(s) 3, 432, 613
ApeKI GCWGC 1 cut(s) 395
ApoI RAATTY 1 cut(s) 627
AspLEI GCGC 2 cut(s) 388, 492
AspS9I GGNCC 1 cut(s) 447
AsuHPI GGTGA 2 cut(s) 37, 530
AsuNHI GCTAGC 1 cut(s) 328
AvaI CYCGRG 2 cut(s) 323, 389
AvaII GGWCC 1 cut(s) 447
BaeI ACNNNNGTAYC 2 cut(s) 187, 220
BalI TGGCCA 1 cut(s) 434
BanI GGYRCC 2 cut(s) 20, 245
BanII GRGCYC 1 cut(s) 624
Bbv12I GWGCWC 1 cut(s) 624
BbvI GCAGC 1 cut(s) 407
BcgI CGANNNNNNTGC 2 cut(s) 235, 269
BciT130I CCWGG 1 cut(s) 617
BciVI GTATCC 2 cut(s) 251, 296
BclI TGATCA 1 cut(s) 145
BfaI CTAG 4 cut(s) 12, 164, 329, 350
BfoI RGCGCY 1 cut(s) 389
BfuI GTATCC 2 cut(s) 251, 296
BisI GCNGC 2 cut(s) 83, 396
BlsI GCNGC 2 cut(s) 84, 397
Bme1390I CCNGG 1 cut(s) 617
Bme18I GGWCC 1 cut(s) 447
BmeT110I CYCGRG 2 cut(s) 323, 389
BmgBI CACGTC 1 cut(s) 187
BmgT120I GGNCC 1 cut(s) 447
BmiI GGNNCC 2 cut(s) 22, 247
BmrFI CCNGG 1 cut(s) 617
BmrI ACTGGG 1 cut(s) 295
BmtI GCTAGC 1 cut(s) 332
BmuI ACTGGG 1 cut(s) 295
BsaBI GATNNNNATC 2 cut(s) 147, 150
BsaHI GRCGYC 1 cut(s) 42
BsaWI WCCGGW 1 cut(s) 72
Bsc4I CCNNNNNNNGG 1 cut(s) 17
Bse118I RCCGGY 1 cut(s) 38
Bse1I ACTGG 1 cut(s) 301
Bse8I GATNNNNATC 2 cut(s) 147, 150
BseBI CCWGG 1 cut(s) 617
BseJI GATNNNNATC 2 cut(s) 147, 150
BseLI CCNNNNNNNGG 1 cut(s) 17
BseMII CTCAG 3 cut(s) 527, 567, 637
BseNI ACTGG 1 cut(s) 301
BseRI GAGGAG 1 cut(s) 617
BseXI GCAGC 1 cut(s) 407
BseYI CCCAGC 1 cut(s) 207
BshFI GGCC 3 cut(s) 5, 434, 615
BshNI GGYRCC 2 cut(s) 20, 245
BsiHKAI GWGCWC 1 cut(s) 624
BsiHKCI CYCGRG 2 cut(s) 323, 389
BsiSI CCGG 2 cut(s) 39, 73
BslFI GGGAC 1 cut(s) 189
BslI CCNNNNNNNGG 1 cut(s) 17
BsmFI GGGAC 1 cut(s) 189
BsnI GGCC 3 cut(s) 5, 434, 615
BsoBI CYCGRG 2 cut(s) 323, 389
Bsp1286I GDGCHC 1 cut(s) 624
Bsp143I GATC 3 cut(s) 145, 403, 595
BspACI CCGC 1 cut(s) 82
BspANI GGCC 3 cut(s) 5, 434, 615
BspCNI CTCAG 3 cut(s) 528, 568, 636
BspLI GGNNCC 2 cut(s) 22, 247
BspOI GCTAGC 1 cut(s) 332
BspPI GGATC 2 cut(s) 398, 590
BspT107I GGYRCC 2 cut(s) 20, 245
BsrFI RCCGGY 1 cut(s) 38
BsrI ACTGG 1 cut(s) 301
BssAI RCCGGY 1 cut(s) 38
BssMI GATC 3 cut(s) 145, 403, 595
BssNI GRCGYC 1 cut(s) 42
Bst2UI CCWGG 1 cut(s) 617
Bst4CI ACNGT 3 cut(s) 52, 203, 346
Bst6I CTCTTC 1 cut(s) 259
BstACI GRCGYC 1 cut(s) 42
BstC8I GCNNGC 2 cut(s) 330, 494
BstDEI CTNAG 4 cut(s) 377, 536, 576, 623
BstH2I RGCGCY 1 cut(s) 389
BstHHI GCGC 2 cut(s) 388, 492
BstKTI GATC 3 cut(s) 148, 406, 598
BstMBI GATC 3 cut(s) 145, 403, 595
BstMWI GCNNNNNNNGC 3 cut(s) 334, 392, 502
BstNI CCWGG 1 cut(s) 617
BstSCI CCNGG 1 cut(s) 615
BstV1I GCAGC 1 cut(s) 407
BsuI GTATCC 2 cut(s) 251, 296
BsuRI GGCC 3 cut(s) 5, 434, 615
BtrI CACGTC 1 cut(s) 187
Cac8I GCNNGC 2 cut(s) 330, 494
CaiI CAGNNNCTG 2 cut(s) 282, 401
CfoI GCGC 2 cut(s) 388, 492
Cfr10I RCCGGY 1 cut(s) 38
Cfr13I GGNCC 1 cut(s) 447
CseI GACGC 1 cut(s) 31
Csp6I GTAC 1 cut(s) 532
CviAII CATG 4 cut(s) 78, 451, 506, 511
CviQI GTAC 1 cut(s) 532
DdeI CTNAG 4 cut(s) 377, 536, 576, 623
DpnI GATC 3 cut(s) 147, 405, 597
DpnII GATC 3 cut(s) 145, 403, 595
EaeI YGGCCR 2 cut(s) 432, 613
Eam1104I CTCTTC 1 cut(s) 259
EarI CTCTTC 1 cut(s) 259
Ecl136II GAGCTC 1 cut(s) 622
Eco24I GRGCYC 1 cut(s) 624
Eco47I GGWCC 1 cut(s) 447
Eco47III AGCGCT 1 cut(s) 387
Eco53kI GAGCTC 1 cut(s) 622
Eco88I CYCGRG 2 cut(s) 323, 389
EcoICRI GAGCTC 1 cut(s) 622
EcoRII CCWGG 1 cut(s) 615
EcoT38I GRGCYC 1 cut(s) 624
FaeI CATG 4 cut(s) 81, 454, 509, 514
FalI AAGNNNNNCTT 2 cut(s) 205, 237
FaqI GGGAC 1 cut(s) 189
FatI CATG 4 cut(s) 77, 450, 505, 510
FbaI TGATCA 1 cut(s) 145
Fnu4HI GCNGC 2 cut(s) 83, 396
FriOI GRGCYC 1 cut(s) 624
Fsp4HI GCNGC 2 cut(s) 83, 396
FspBI CTAG 4 cut(s) 12, 164, 329, 350
GlaI GCGC 2 cut(s) 387, 491
GluI GCNGC 2 cut(s) 83, 396
GsaI CCCAGC 1 cut(s) 211
HaeII RGCGCY 1 cut(s) 389
HaeIII GGCC 3 cut(s) 5, 434, 615
HapII CCGG 2 cut(s) 39, 73
HgaI GACGC 1 cut(s) 31
HhaI GCGC 2 cut(s) 388, 492
Hin1I GRCGYC 1 cut(s) 42
Hin1II CATG 4 cut(s) 81, 454, 509, 514
Hin6I GCGC 2 cut(s) 386, 490
HinP1I GCGC 2 cut(s) 386, 490
HincII GTYRAC 2 cut(s) 55, 190
HindII GTYRAC 2 cut(s) 55, 190
HindIII AAGCTT 1 cut(s) 214
HinfI GANTC 1 cut(s) 578
HpaII CCGG 2 cut(s) 39, 73
HphI GGTGA 2 cut(s) 37, 530
Hpy166II GTNNAC 2 cut(s) 55, 190
Hpy188I TCNGA 3 cut(s) 257, 577, 626
Hpy188III TCNNGA 4 cut(s) 155, 164, 323, 599
Hpy8I GTNNAC 2 cut(s) 55, 190
HpyAV CCTTC 4 cut(s) 142, 329, 507, 509
HpyCH4III ACNGT 3 cut(s) 52, 203, 346
HpyCH4IV ACGT 1 cut(s) 186
HpyCH4V TGCA 5 cut(s) 123, 129, 364, 459, 482
HpyF10VI GCNNNNNNNGC 3 cut(s) 334, 392, 502
HpyF3I CTNAG 4 cut(s) 377, 536, 576, 623
HpySE526I ACGT 1 cut(s) 186
Hsp92I GRCGYC 1 cut(s) 42
Hsp92II CATG 4 cut(s) 81, 454, 509, 514
HspAI GCGC 2 cut(s) 386, 490
Ksp22I TGATCA 1 cut(s) 145
Kzo9I GATC 3 cut(s) 145, 403, 595
LmnI GCTCC 1 cut(s) 619
LpnPI CCDG 9 cut(s) 52, 86, 221, 314, 453, 548, 602, 612, 629
Lsp1109I GCAGC 1 cut(s) 407
MaeI CTAG 4 cut(s) 12, 164, 329, 350
MaeII ACGT 1 cut(s) 186
MaeIII GTNAC 1 cut(s) 43
MalI GATC 3 cut(s) 147, 405, 597
MboI GATC 3 cut(s) 145, 403, 595
MboII GAAGA 4 cut(s) 80, 152, 276, 408
MfeI CAATTG 1 cut(s) 124
MhlI GDGCHC 1 cut(s) 624
MlsI TGGCCA 1 cut(s) 434
MluCI AATT 5 cut(s) 124, 312, 544, 571, 627
MluNI TGGCCA 1 cut(s) 434
MlyI GAGTC 1 cut(s) 587
MmeI TCCRAC 2 cut(s) 252, 330
MnlI CCTC 4 cut(s) 260, 307, 385, 595
Mox20I TGGCCA 1 cut(s) 434
MscI TGGCCA 1 cut(s) 434
Msp20I TGGCCA 1 cut(s) 434
MspI CCGG 2 cut(s) 39, 73
MspR9I CCNGG 1 cut(s) 617
MunI CAATTG 1 cut(s) 124
MvaI CCWGG 1 cut(s) 617
MwoI GCNNNNNNNGC 3 cut(s) 334, 392, 502
NdeII GATC 3 cut(s) 145, 403, 595
NheI GCTAGC 1 cut(s) 328
NlaIII CATG 4 cut(s) 81, 454, 509, 514
NlaIV GGNNCC 2 cut(s) 22, 247
NmuCI GTSAC 1 cut(s) 43
PaeR7I CTCGAG 2 cut(s) 323, 389
PkrI GCNGC 2 cut(s) 84, 397
PleI GAGTC 1 cut(s) 586
PpsI GAGTC 1 cut(s) 586
Psp124BI GAGCTC 1 cut(s) 624
Psp6I CCWGG 1 cut(s) 615
PspFI CCCAGC 1 cut(s) 207
PspGI CCWGG 1 cut(s) 615
PspN4I GGNNCC 2 cut(s) 22, 247
PspPI GGNCC 1 cut(s) 447
PspXI VCTCGAGB 1 cut(s) 389
PstNI CAGNNNCTG 2 cut(s) 282, 401
RsaI GTAC 1 cut(s) 533
RsaNI GTAC 1 cut(s) 532
SacI GAGCTC 1 cut(s) 624
SatI GCNGC 2 cut(s) 83, 396
Sau3AI GATC 3 cut(s) 145, 403, 595
Sau96I GGNCC 1 cut(s) 447
SchI GAGTC 1 cut(s) 587
ScrFI CCNGG 1 cut(s) 617
SduI GDGCHC 1 cut(s) 624
Sfr274I CTCGAG 2 cut(s) 323, 389
SgrAI CRCCGGYG 1 cut(s) 38
SinI GGWCC 1 cut(s) 447
SlaI CTCGAG 2 cut(s) 323, 389
SmlI CTYRAG 2 cut(s) 323, 389
SmoI CTYRAG 2 cut(s) 323, 389
Sse9I AATT 5 cut(s) 124, 312, 544, 571, 627
SsiI CCGC 1 cut(s) 82
SspMI CTAG 4 cut(s) 12, 164, 329, 350
SstI GAGCTC 1 cut(s) 624
StyD4I CCNGG 1 cut(s) 615
TaaI ACNGT 3 cut(s) 52, 203, 346
TaiI ACGT 1 cut(s) 189
TaqI TCGA 3 cut(s) 102, 324, 390
TaqII GACCGA 1 cut(s) 630
TasI AATT 5 cut(s) 124, 312, 544, 571, 627
TauI GCSGC 1 cut(s) 85
TseFI GTSAC 1 cut(s) 43
TseI GCWGC 1 cut(s) 395
Tsp45I GTSAC 1 cut(s) 43
TspDTI ATGAA 1 cut(s) 575
VpaK11BI GGWCC 1 cut(s) 447
XapI RAATTY 1 cut(s) 627
XbaI TCTAGA 1 cut(s) 163
XhoI CTCGAG 2 cut(s) 323, 389
XspI CTAG 4 cut(s) 12, 164, 329, 350
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.