AT5G66170

Rhodanese-like domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0012191
Physical Location & Seq
Forward (+)
26447642 .. 26448776
1135 bp
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UTR
Exon/CDS
Intron
AT5G66170.3

Sequence Viewer

Length: 411 bp
ATGTCTCAATCAATCTCCTCCAGCACAAAGGCAGAAGAAGTTGTTAGTGTAGATGTGAGCCAAGCCAAGACTCTCCTCCAGTCTGGGCATCAATATCTTGACGTTAGGACTCAGGACGAGTTTAGGAGAGGCCATTGTGAGGCAGCTAAGATCGTCAACATTCCCTACATGCTCAACACACCTCAAGGTAGAGTGAAGAATCAAGAGTTCTTGGAGCAAGTATCTTCTCTTCTAAACCCAGCTGATGATATCCTTGTGGGTTGTCAGAGTGGAGCCAGATCCTTAAAAGCCACAACTGAACTTGTTGCTGCAGTAAGGTTACAAGAAAGTGAGAAACGTGGGAGGTGGCTACTTGGCTTGGGTGGATCACAGCTTTCCCATCAACACGGAGGAGGAGGAGCCATCTGCTAA

Protein Analysis

136

Amino Acids

14.71

Weight (kDa)

6.9

Isoelectric Point (pI)

66.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rhodanese PF00581 20 - 121 6.7e-15 Rhodanese-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012191)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G66170 AT5G66170 AT5G66170
fragaria_vesca FvH4_1g11640 FvH4_1g11640
malus_domestica MD02G1129500.v1.1 MD15G1243800.v1.1
prunus_persica Prupe.7G170100_v2.0.a1 Prupe.7G170100_v2.0.a1
pyrus_communis pycom02g10140 pycom15g21470
rosa_chinensis RchiOBHm_Chr2g0099231
rosa_laevigata RLG00000016863
rosa_roxburghii Rroxscaffold_2G00143300
rosa_samantha Rh2AG127200 Rh2BG130200 Rh2CG131400 Rh2DG131800
rosa_wichuraiana Rw2G009810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 273, 373
AfiI CCNNNNNNNGG 1 cut(s) 139
AluBI AGCT 3 cut(s) 146, 242, 373
AluI AGCT 3 cut(s) 146, 242, 373
Alw26I GTCTC 1 cut(s) 9
AlwI GGATC 2 cut(s) 273, 373
AoxI GGCC 1 cut(s) 130
ApeKI GCWGC 2 cut(s) 143, 308
BarI GAAGNNNNNNTAC 2 cut(s) 213, 245
BbvI GCAGC 2 cut(s) 155, 295
BccI CCATC 2 cut(s) 387, 410
BcoDI GTCTC 1 cut(s) 9
BfmI CTRYAG 1 cut(s) 309
BisI GCNGC 2 cut(s) 144, 309
BlsI GCNGC 2 cut(s) 145, 310
BmiI GGNNCC 2 cut(s) 274, 400
BmsI GCATC 1 cut(s) 97
BpmI CTGGAG 2 cut(s) 4, 62
BpuEI CTTGAG 1 cut(s) 168
Bsc4I CCNNNNNNNGG 1 cut(s) 139
Bse1I ACTGG 1 cut(s) 79
BseLI CCNNNNNNNGG 1 cut(s) 139
BseMII CTCAG 1 cut(s) 125
BseNI ACTGG 1 cut(s) 79
BseRI GAGGAG 5 cut(s) 7, 65, 405, 408, 411
BseXI GCAGC 2 cut(s) 155, 295
BseYI CCCAGC 1 cut(s) 238
BshFI GGCC 1 cut(s) 132
BslI CCNNNNNNNGG 1 cut(s) 139
BsmAI GTCTC 1 cut(s) 9
BsnI GGCC 1 cut(s) 132
Bsp143I GATC 3 cut(s) 150, 278, 365
BspANI GGCC 1 cut(s) 132
BspCNI CTCAG 1 cut(s) 124
BspLI GGNNCC 2 cut(s) 274, 400
BspMAI CTGCAG 1 cut(s) 313
BspPI GGATC 2 cut(s) 273, 373
BsrI ACTGG 1 cut(s) 79
BssMI GATC 3 cut(s) 150, 278, 365
Bst6I CTCTTC 1 cut(s) 234
BstDEI CTNAG 2 cut(s) 111, 147
BstKTI GATC 3 cut(s) 153, 281, 368
BstMAI GTCTC 1 cut(s) 9
BstMBI GATC 3 cut(s) 150, 278, 365
BstNSI RCATGY 1 cut(s) 172
BstSFI CTRYAG 1 cut(s) 309
BstV1I GCAGC 2 cut(s) 155, 295
BstX2I RGATCY 1 cut(s) 278
BstYI RGATCY 1 cut(s) 278
BsuRI GGCC 1 cut(s) 132
CviAII CATG 1 cut(s) 169
DdeI CTNAG 2 cut(s) 111, 147
DpnI GATC 3 cut(s) 152, 280, 367
DpnII GATC 3 cut(s) 150, 278, 365
Eam1104I CTCTTC 1 cut(s) 234
EarI CTCTTC 1 cut(s) 234
Eco32I GATATC 1 cut(s) 250
EcoRV GATATC 1 cut(s) 250
FaeI CATG 1 cut(s) 172
FaiI YATR 1 cut(s) 170
FatI CATG 1 cut(s) 168
Fnu4HI GCNGC 2 cut(s) 144, 309
Fsp4HI GCNGC 2 cut(s) 144, 309
GluI GCNGC 2 cut(s) 144, 309
GsaI CCCAGC 1 cut(s) 242
GsuI CTGGAG 2 cut(s) 4, 62
HaeIII GGCC 1 cut(s) 132
Hin1II CATG 1 cut(s) 172
HincII GTYRAC 1 cut(s) 157
HindII GTYRAC 1 cut(s) 157
HinfI GANTC 3 cut(s) 70, 109, 199
Hpy166II GTNNAC 1 cut(s) 157
Hpy188I TCNGA 1 cut(s) 267
Hpy188III TCNNGA 3 cut(s) 98, 113, 203
Hpy8I GTNNAC 1 cut(s) 157
HpyCH4IV ACGT 2 cut(s) 102, 337
HpyCH4V TGCA 1 cut(s) 311
HpyF3I CTNAG 2 cut(s) 111, 147
HpySE526I ACGT 2 cut(s) 102, 337
Hsp92II CATG 1 cut(s) 172
Kzo9I GATC 3 cut(s) 150, 278, 365
LmnI GCTCC 3 cut(s) 214, 272, 398
LpnPI CCDG 6 cut(s) 34, 69, 92, 98, 252, 289
Lsp1109I GCAGC 2 cut(s) 155, 295
LweI GCATC 1 cut(s) 97
MaeII ACGT 2 cut(s) 102, 337
MaeIII GTNAC 1 cut(s) 318
MalI GATC 3 cut(s) 152, 280, 367
MboI GATC 3 cut(s) 150, 278, 365
MboII GAAGA 4 cut(s) 47, 208, 216, 221
MflI RGATCY 1 cut(s) 278
MlyI GAGTC 2 cut(s) 64, 103
MnlI CCTC 9 cut(s) 28, 86, 122, 133, 192, 336, 383, 386, 389
MseI TTAA 1 cut(s) 284
MspA1I CMGCKG 1 cut(s) 242
NdeII GATC 3 cut(s) 150, 278, 365
NlaIII CATG 1 cut(s) 172
NlaIV GGNNCC 2 cut(s) 274, 400
NspI RCATGY 1 cut(s) 172
PfeI GAWTC 1 cut(s) 199
PkrI GCNGC 2 cut(s) 145, 310
PleI GAGTC 2 cut(s) 64, 103
PpsI GAGTC 2 cut(s) 64, 103
PspFI CCCAGC 1 cut(s) 238
PspN4I GGNNCC 2 cut(s) 274, 400
PstI CTGCAG 1 cut(s) 313
PsuI RGATCY 1 cut(s) 278
PvuII CAGCTG 1 cut(s) 242
SaqAI TTAA 1 cut(s) 284
SatI GCNGC 2 cut(s) 144, 309
Sau3AI GATC 3 cut(s) 150, 278, 365
SchI GAGTC 2 cut(s) 64, 103
SetI ASST 9 cut(s) 105, 148, 184, 190, 244, 320, 340, 347, 375
SfaNI GCATC 1 cut(s) 97
SfcI CTRYAG 1 cut(s) 309
SmlI CTYRAG 1 cut(s) 183
SmoI CTYRAG 1 cut(s) 183
TaiI ACGT 2 cut(s) 105, 340
TfiI GAWTC 1 cut(s) 199
Tru1I TTAA 1 cut(s) 284
Tru9I TTAA 1 cut(s) 284
TseI GCWGC 2 cut(s) 143, 308
TspGWI ACGGA 1 cut(s) 402
XceI RCATGY 1 cut(s) 172
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.