MD02G1129500.v1.1

Thiosulfate sulfurtransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0012191
Physical Location & Seq
Reverse (-)
10467477 .. 10469133
1657 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1129500.v1.1.491

Sequence Viewer

Length: 471 bp
ATGGGTTCTATTGGAAGCTCGGAATCGGAAGTTGTCACCGTTGATGTTCAAGAAGCAAAACATCTGCTCGAATCTGGCTATGGTTATTTGGAAGTTAGGACGGAGGAGGAGTACAAGAAAGGGCATGTGGATACAAAGAAGATTCTGAACATTCCTTACATGTTCAATACACCTAAAGGTATGGTGAAAAATCCCCAGTTTTTGGAAGAGGTTTCATCTGCCTGCAACAAAGATGACCTTATTGTTGTGGGTTGTCAAAGTGGTGTTAGATCCCTTTATGCAACTGCTGATCTTCTGGCTGCTGGTTTCAAGAATGTGAGCAACATGGGAGGAGGCTATCTCGCTTGGATCGAGCATGATTTTCCGGTTACTAAGCCGGAAGACGCTGACCGGAAAAAGCCAGAAGATGTTGTGCAACCGAAAAAGGCAGAAGATGTAGACCAGCAGAAGAAAGCAAAAGAAGAGCTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004792 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0016740 GO:0016782 GO:0016783 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rhodanese

Protein Analysis

157

Amino Acids

17.3

Weight (kDa)

5.04

Isoelectric Point (pI)

46.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rhodanese PF00581 16 - 118 7.1e-14 Rhodanese-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012191)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G66170 AT5G66170 AT5G66170
fragaria_vesca FvH4_1g11640 FvH4_1g11640
malus_domestica MD02G1129500.v1.1 MD15G1243800.v1.1
prunus_persica Prupe.7G170100_v2.0.a1 Prupe.7G170100_v2.0.a1
pyrus_communis pycom02g10140 pycom15g21470
rosa_chinensis RchiOBHm_Chr2g0099231
rosa_laevigata RLG00000016863
rosa_roxburghii Rroxscaffold_2G00143300
rosa_samantha Rh2AG127200 Rh2BG130200 Rh2CG131400 Rh2DG131800
rosa_wichuraiana Rw2G009810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 202
AccI GTMKAC 1 cut(s) 438
AclWI GGATC 2 cut(s) 264, 356
AfaI GTAC 1 cut(s) 113
AfiI CCNNNNNNNGG 1 cut(s) 202
AflIII ACRYGT 1 cut(s) 159
AgsI TTSAA 3 cut(s) 50, 166, 310
AluBI AGCT 2 cut(s) 18, 466
AluI AGCT 2 cut(s) 18, 466
Alw21I GWGCWC 1 cut(s) 468
AlwI GGATC 2 cut(s) 264, 356
ApeKI GCWGC 1 cut(s) 299
AsuHPI GGTGA 2 cut(s) 28, 196
BanII GRGCYC 1 cut(s) 468
BbsI GAAGAC 1 cut(s) 387
Bbv12I GWGCWC 1 cut(s) 468
BbvI GCAGC 1 cut(s) 286
BciVI GTATCC 1 cut(s) 124
BfaI CTAG 1 cut(s) 469
BfuI GTATCC 1 cut(s) 124
BisI GCNGC 1 cut(s) 300
BlsI GCNGC 1 cut(s) 301
BmrI ACTGGG 1 cut(s) 190
BmuI ACTGGG 1 cut(s) 190
BpiI GAAGAC 1 cut(s) 387
BplI GAGNNNNNCTC 2 cut(s) 324, 356
BsaWI WCCGGW 2 cut(s) 364, 390
BsaXI ACNNNNNCTCC 2 cut(s) 95, 125
Bsc4I CCNNNNNNNGG 1 cut(s) 202
Bse1I ACTGG 1 cut(s) 196
BseLI CCNNNNNNNGG 1 cut(s) 202
BseNI ACTGG 1 cut(s) 196
BseRI GAGGAG 3 cut(s) 119, 122, 345
BseXI GCAGC 1 cut(s) 286
BsiHKAI GWGCWC 1 cut(s) 468
BsiSI CCGG 3 cut(s) 365, 377, 391
BslI CCNNNNNNNGG 1 cut(s) 202
Bsp1286I GDGCHC 1 cut(s) 468
Bsp143I GATC 3 cut(s) 269, 289, 348
BspPI GGATC 2 cut(s) 264, 356
BspQI GCTCTTC 1 cut(s) 456
BsrI ACTGG 1 cut(s) 196
BssMI GATC 3 cut(s) 269, 289, 348
Bst4CI ACNGT 1 cut(s) 40
Bst6I CTCTTC 2 cut(s) 201, 456
BstC8I GCNNGC 1 cut(s) 223
BstDEI CTNAG 1 cut(s) 372
BstKTI GATC 3 cut(s) 272, 292, 351
BstMBI GATC 3 cut(s) 269, 289, 348
BstNSI RCATGY 2 cut(s) 128, 163
BstV1I GCAGC 1 cut(s) 286
BstV2I GAAGAC 1 cut(s) 387
BstX2I RGATCY 1 cut(s) 269
BstYI RGATCY 1 cut(s) 269
BsuI GTATCC 1 cut(s) 124
Cac8I GCNNGC 1 cut(s) 223
CseI GACGC 1 cut(s) 392
Csp6I GTAC 1 cut(s) 112
CviAII CATG 4 cut(s) 125, 160, 325, 356
CviJI RGCY 7 cut(s) 18, 78, 299, 336, 376, 400, 466
CviKI_1 RGCY 7 cut(s) 18, 78, 299, 336, 376, 400, 466
CviQI GTAC 1 cut(s) 112
DdeI CTNAG 1 cut(s) 372
DpnI GATC 3 cut(s) 271, 291, 350
DpnII GATC 3 cut(s) 269, 289, 348
Eam1104I CTCTTC 2 cut(s) 201, 456
EarI CTCTTC 2 cut(s) 201, 456
Ecl136II GAGCTC 1 cut(s) 466
Eco24I GRGCYC 1 cut(s) 468
Eco53kI GAGCTC 1 cut(s) 466
EcoICRI GAGCTC 1 cut(s) 466
EcoT38I GRGCYC 1 cut(s) 468
FaeI CATG 4 cut(s) 128, 163, 328, 359
FaiI YATR 7 cut(s) 81, 126, 161, 182, 279, 326, 357
FalI AAGNNNNNCTT 2 cut(s) 222, 254
FatI CATG 4 cut(s) 124, 159, 324, 355
FblI GTMKAC 1 cut(s) 438
Fnu4HI GCNGC 1 cut(s) 300
FriOI GRGCYC 1 cut(s) 468
Fsp4HI GCNGC 1 cut(s) 300
FspBI CTAG 1 cut(s) 469
GluI GCNGC 1 cut(s) 300
HapII CCGG 3 cut(s) 365, 377, 391
HgaI GACGC 1 cut(s) 392
Hin1II CATG 4 cut(s) 128, 163, 328, 359
HinfI GANTC 3 cut(s) 23, 71, 142
HpaII CCGG 3 cut(s) 365, 377, 391
HphI GGTGA 2 cut(s) 28, 196
Hpy166II GTNNAC 1 cut(s) 439
Hpy188I TCNGA 3 cut(s) 22, 28, 147
Hpy188III TCNNGA 2 cut(s) 50, 310
Hpy8I GTNNAC 1 cut(s) 439
HpyCH4III ACNGT 1 cut(s) 40
HpyCH4V TGCA 3 cut(s) 225, 281, 415
HpyF3I CTNAG 1 cut(s) 372
Hsp92II CATG 4 cut(s) 128, 163, 328, 359
Kzo9I GATC 3 cut(s) 269, 289, 348
LguI GCTCTTC 1 cut(s) 456
Lsp1109I GCAGC 1 cut(s) 286
MaeI CTAG 1 cut(s) 469
MaeIII GTNAC 2 cut(s) 34, 367
MalI GATC 3 cut(s) 271, 291, 350
MboI GATC 3 cut(s) 269, 289, 348
MboII GAAGA 7 cut(s) 151, 218, 284, 392, 416, 443, 460
MflI RGATCY 1 cut(s) 269
MhlI GDGCHC 1 cut(s) 468
MnlI CCTC 5 cut(s) 97, 100, 202, 323, 326
MspI CCGG 3 cut(s) 365, 377, 391
NdeII GATC 3 cut(s) 269, 289, 348
NlaIII CATG 4 cut(s) 128, 163, 328, 359
NmuCI GTSAC 1 cut(s) 34
NspI RCATGY 2 cut(s) 128, 163
PciI ACATGT 1 cut(s) 159
PciSI GCTCTTC 1 cut(s) 456
PcsI WCGNNNNNNNCGW 1 cut(s) 348
PfeI GAWTC 3 cut(s) 23, 71, 142
PflMI CCANNNNNTGG 1 cut(s) 202
PkrI GCNGC 1 cut(s) 301
PscI ACATGT 1 cut(s) 159
Psp124BI GAGCTC 1 cut(s) 468
PsrI GAACNNNNNNTAC 2 cut(s) 140, 172
PsuI RGATCY 1 cut(s) 269
RsaI GTAC 1 cut(s) 113
RsaNI GTAC 1 cut(s) 112
SacI GAGCTC 1 cut(s) 468
SapI GCTCTTC 1 cut(s) 456
SatI GCNGC 1 cut(s) 300
Sau3AI GATC 3 cut(s) 269, 289, 348
SduI GDGCHC 1 cut(s) 468
SetI ASST 6 cut(s) 20, 175, 181, 213, 240, 468
SspMI CTAG 1 cut(s) 469
SstI GAGCTC 1 cut(s) 468
TaaI ACNGT 1 cut(s) 40
TaqI TCGA 2 cut(s) 69, 351
TatI WGTACW 1 cut(s) 111
TfiI GAWTC 3 cut(s) 23, 71, 142
TseFI GTSAC 1 cut(s) 34
TseI GCWGC 1 cut(s) 299
Tsp45I GTSAC 1 cut(s) 34
TspDTI ATGAA 1 cut(s) 204
TspGWI ACGGA 1 cut(s) 116
Van91I CCANNNNNTGG 1 cut(s) 202
XceI RCATGY 2 cut(s) 128, 163
XmiI GTMKAC 1 cut(s) 438
XspI CTAG 1 cut(s) 469
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.