Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0014596

Physical Location & Seq

Genomic Coordinates Forward (+)

26510146 .. 26511844

Gene Structure 1699 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT5G66360.2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1059 bp

ATGATTCTTCGATTGAAAGACCAAACGCTCATCAAAATCAATTCAACTCGTTCGTACTTATCTTCCTTAGTTTTCCGACGAGATTCTCACTCGCAGGCTCGGACAAAACCCGATCACGACCGCCGGAGAAGAGGATACGAGAGGGATGTGAGAATCGAGGAGAAGAAGGAACACGATGGATTGTTTCTGTGCAAGAGCAAAGGCCAACACCTTCTTACCAACACTAGAATCCTCGACTCCATTGTTCGAAGCTCCGATATCAGGCCGACTGATACAGTCTTGGAGATCGGACCTGGCACTGGAAATCTCACGATGAAACTTCTTGAAGCTGCTCAGAATGTCGTAGCCGTCGAGCTAGATAAGCGTATGGTGGAGATTCTACGCAAAAGGGTTTCTGACCATGGTTTTGCAGATAAACTTACGATTATTCAAAAAGATGTTCTGAAGACAGATTTTCCTCACTTTGATCTCGTGGTTGCAAATATTCCTTACAACATATCTTCCCCTCTTGTGGCCAAGCTTGTATACGGCTCCAATACTTTTCGAAGCGCCACACTCTTGCTTCAAAAGGAATTCTCCCGCCGGCTCCTGGCAAATCCAGGAGATTCCGATTTCAATAGATTGGCGGTGAACGTTAAGATTACACCAAAGGAGATAATACCGGATGTTAATGTTCAAGAATGGTTGGCATTCACTCGCACGTGTTTCGGGAAGAAGAATAAGACGCTTGGTTCCATGTTTAGGCAGAAGAAAAAGGTCATGGAATTGCAGAGTTTGTCTGCAGGGAGGCATGGCTCGAATGTTGAGGTTATGAATCAGACAGGTGGTGATTCTGACAGTGATGTTGAAGAAGATGGGAAAGATGATTTGTTGTGTTTGGATACAGATGCAAGTATGTTCAAGGAAAGGGTAATTGAAATTCTGAGAACAAATGGGTTCGAAGAGAAGAGACCATCCAAGCTTTCACATCGTGAGTTGTTACATTTGCTTTCTTTGTTTAACCAAGCTGGAATTTTTTTCCATGATATTACATCATTACAAATGGATCTACACGAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000154 GO:0000179 GO:0001510 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005759 GO:0006139 GO:0006364 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008168 GO:0008170 GO:0008173 GO:0008649 GO:0008757 GO:0009116 GO:0009119 GO:0009451 GO:0009987 GO:0010467 GO:0016070 GO:0016072 GO:0016433 GO:0016740 GO:0016741 GO:0017144 GO:0022613 GO:0031167 GO:0031974 GO:0032259 GO:0034470 GO:0034641 GO:0034660 GO:0042254 GO:0042278 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043414 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044281 GO:0044422 GO:0044424 GO:0044429 GO:0044444 GO:0044446 GO:0044464 GO:0046085 GO:0046128 GO:0046483 GO:0055086 GO:0070013 GO:0071704 GO:0071840 GO:0072521 GO:0090304 GO:0140098 GO:0140102 GO:1901135 GO:1901360 GO:1901564 GO:1901657

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

RrnaAD

Protein Analysis

352

Amino Acids

40.17

Weight (kDa)

9.08

Isoelectric Point (pI)

43.1

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RrnaAD	PF00398	66 - 289	4.7e-63	Ribosomal RNA adenine dimethylase
MTS	PF05175	89 - 169	5.2e-07	Methyltransferase small domain
Methyltransf_31	PF13847	90 - 161	2.9e-06	Methyltransferase domain
Methyltransf_25	PF13649	93 - 161	3e-11	Methyltransferase domain
Methyltransf_12	PF08242	94 - 161	2.8e-09	Methyltransferase domain
Methyltransf_11	PF08241	94 - 161	4.1e-08	Methyltransferase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0014596)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G66360 AT5G66360
fragaria_vesca	FvH4_1g12340
malus_domestica	MD02G1137600.v1.1 MD15G1251400.v1.1
prunus_persica	Prupe.7G163000_v2.0.a1
pyrus_communis	pycom02g10890
rosa_chinensis	RchiOBHm_Chr2g0100161
rosa_laevigata	RLG00000016942
rosa_multiflora	Rmu_sc0018554.1_g000006
rosa_roxburghii	Rroxscaffold_2G00142510
rosa_rugosa	Rorug02G0086100
rosa_samantha	Rh2AG135200 Rh2BG138600 Rh2CG140400 Rh2DG140600
rosa_wichuraiana	Rw2G010550

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	1 cut(s)	525
AciI	CCGC	3 cut(s)	121, 580, 626
AclI	AACGTT	1 cut(s)	633
AclWI	GGATC	1 cut(s)	1053
AcoI	YGGCCR	1 cut(s)	513
AcsI	RAATTY	3 cut(s)	572, 918, 1011
AcuI	CTGAAG	1 cut(s)	464
AcvI	CACGTG	1 cut(s)	702
AdeI	CACNNNGTG	1 cut(s)	971
AfaI	GTAC	1 cut(s)	56
AfiI	CCNNNNNNNGG	5 cut(s)	261, 299, 511, 589, 741
AflIII	ACRYGT	1 cut(s)	701
AjnI	CCWGG	3 cut(s)	292, 588, 598
AluBI	AGCT	6 cut(s)	252, 329, 355, 520, 961, 1007
AluI	AGCT	6 cut(s)	252, 329, 355, 520, 961, 1007
Alw26I	GTCTC	1 cut(s)	943
AlwI	GGATC	1 cut(s)	1053
AoxI	GGCC	3 cut(s)	202, 263, 513
ApeKI	GCWGC	1 cut(s)	329
ApoI	RAATTY	3 cut(s)	572, 918, 1011
ArsI	GACNNNNNNTTYG	2 cut(s)	389, 421
AspLEI	GCGC	1 cut(s)	551
AspS9I	GGNCC	1 cut(s)	290
AsuHPI	GGTGA	2 cut(s)	640, 839
AsuII	TTCGAA	3 cut(s)	247, 544, 939
AvaII	GGWCC	1 cut(s)	290
BalI	TGGCCA	1 cut(s)	515
BauI	CACGAG	1 cut(s)	470
BbrPI	CACGTG	1 cut(s)	702
BbsI	GAAGAC	1 cut(s)	452
BbvI	GCAGC	1 cut(s)	316
BccI	CCATC	3 cut(s)	170, 848, 961
BceAI	ACGGC	2 cut(s)	332, 544
BcgI	CGANNNNNNTGC	2 cut(s)	688, 722
BciT130I	CCWGG	3 cut(s)	294, 590, 600
BciVI	GTATCC	2 cut(s)	128, 874
BcoDI	GTCTC	1 cut(s)	943
BfaI	CTAG	2 cut(s)	225, 356
BfmI	CTRYAG	1 cut(s)	780
BfoI	RGCGCY	1 cut(s)	552
BfuI	GTATCC	2 cut(s)	128, 874
BisI	GCNGC	1 cut(s)	330
BlsI	GCNGC	1 cut(s)	331
Bme1390I	CCNGG	3 cut(s)	294, 590, 600
Bme18I	GGWCC	1 cut(s)	290
BmgT120I	GGNCC	1 cut(s)	290
BmiI	GGNNCC	3 cut(s)	532, 587, 733
BmrFI	CCNGG	3 cut(s)	294, 590, 600
BmsI	GCATC	1 cut(s)	877
BpiI	GAAGAC	1 cut(s)	452
Bpu14I	TTCGAA	3 cut(s)	247, 544, 939
BsaAI	YACGTR	1 cut(s)	702
BsaI	GGTCTC	1 cut(s)	943
BsaJI	CCNNGG	1 cut(s)	400
BsaWI	WCCGGW	1 cut(s)	661
Bsc4I	CCNNNNNNNGG	5 cut(s)	261, 299, 511, 589, 741
Bse118I	RCCGGY	1 cut(s)	582
Bse1I	ACTGG	1 cut(s)	304
BseBI	CCWGG	3 cut(s)	294, 590, 600
BseDI	CCNNGG	1 cut(s)	400
BseGI	GGATG	3 cut(s)	151, 670, 953
BseLI	CCNNNNNNNGG	5 cut(s)	261, 299, 511, 589, 741
BseMII	CTCAG	2 cut(s)	347, 914
BseNI	ACTGG	1 cut(s)	304
BseRI	GAGGAG	1 cut(s)	173
BseXI	GCAGC	1 cut(s)	316
Bsh1285I	CGRYCG	1 cut(s)	121
BshFI	GGCC	3 cut(s)	204, 265, 515
BsiEI	CGRYCG	1 cut(s)	121
BsiSI	CCGG	3 cut(s)	124, 583, 662
BslI	CCNNNNNNNGG	5 cut(s)	261, 299, 511, 589, 741
BsmAI	GTCTC	1 cut(s)	943
BsmI	GAATGC	1 cut(s)	689
BsnI	GGCC	3 cut(s)	204, 265, 515
Bso31I	GGTCTC	1 cut(s)	943
Bsp119I	TTCGAA	3 cut(s)	247, 544, 939
Bsp143I	GATC	4 cut(s)	112, 285, 466, 1045
Bsp19I	CCATGG	1 cut(s)	400
BspACI	CCGC	3 cut(s)	121, 580, 626
BspANI	GGCC	3 cut(s)	204, 265, 515
BspCNI	CTCAG	2 cut(s)	346, 915
BspLI	GGNNCC	3 cut(s)	532, 587, 733
BspMAI	CTGCAG	1 cut(s)	784
BspPI	GGATC	1 cut(s)	1053
BspT104I	TTCGAA	3 cut(s)	247, 544, 939
BspTNI	GGTCTC	1 cut(s)	943
BsrFI	RCCGGY	1 cut(s)	582
BsrI	ACTGG	1 cut(s)	304
BssAI	RCCGGY	1 cut(s)	582
BssECI	CCNNGG	1 cut(s)	400
BssMI	GATC	4 cut(s)	112, 285, 466, 1045
BssNAI	GTATAC	1 cut(s)	526
BssSI	CACGAG	1 cut(s)	470
BssT1I	CCWWGG	1 cut(s)	400
Bst1107I	GTATAC	1 cut(s)	526
Bst2BI	CACGAG	1 cut(s)	470
Bst2UI	CCWGG	3 cut(s)	294, 590, 600
Bst4CI	ACNGT	2 cut(s)	277, 839
Bst6I	CTCTTC	3 cut(s)	124, 936, 941
BstBAI	YACGTR	1 cut(s)	702
BstBI	TTCGAA	3 cut(s)	247, 544, 939
BstC8I	GCNNGC	2 cut(s)	96, 584
BstDEI	CTNAG	3 cut(s)	67, 333, 923
BstDSI	CCRYGG	1 cut(s)	400
BstF5I	GGATG	3 cut(s)	151, 670, 953
BstH2I	RGCGCY	1 cut(s)	552
BstHHI	GCGC	1 cut(s)	551
BstKTI	GATC	4 cut(s)	115, 288, 469, 1048
BstMAI	GTCTC	1 cut(s)	943
BstMBI	GATC	4 cut(s)	112, 285, 466, 1045
BstMCI	CGRYCG	1 cut(s)	121
BstMWI	GCNNNNNNNGC	1 cut(s)	361
BstNI	CCWGG	3 cut(s)	294, 590, 600
BstSCI	CCNGG	3 cut(s)	292, 588, 598
BstSFI	CTRYAG	1 cut(s)	780
BstV1I	GCAGC	1 cut(s)	316
BstV2I	GAAGAC	1 cut(s)	452
BstX2I	RGATCY	1 cut(s)	1045
BstYI	RGATCY	1 cut(s)	1045
BstZ17I	GTATAC	1 cut(s)	526
BsuI	GTATCC	2 cut(s)	128, 874
BsuRI	GGCC	3 cut(s)	204, 265, 515
BtgI	CCRYGG	1 cut(s)	400
BtsCI	GGATG	3 cut(s)	151, 670, 953
BtsIMutI	CAGTG	2 cut(s)	297, 844
Cac8I	GCNNGC	2 cut(s)	96, 584
CfoI	GCGC	1 cut(s)	551
Cfr10I	RCCGGY	1 cut(s)	582
Cfr13I	GGNCC	1 cut(s)	290
CseI	GACGC	1 cut(s)	733
Csp6I	GTAC	1 cut(s)	55
CviAII	CATG	5 cut(s)	401, 736, 760, 791, 1022
CviQI	GTAC	1 cut(s)	55
DdeI	CTNAG	3 cut(s)	67, 333, 923
DpnI	GATC	4 cut(s)	114, 287, 468, 1047
DpnII	GATC	4 cut(s)	112, 285, 466, 1045
DraIII	CACNNNGTG	1 cut(s)	971
EaeI	YGGCCR	1 cut(s)	513
Eam1104I	CTCTTC	3 cut(s)	124, 936, 941
EarI	CTCTTC	3 cut(s)	124, 936, 941
Eco130I	CCWWGG	1 cut(s)	400
Eco31I	GGTCTC	1 cut(s)	943
Eco32I	GATATC	1 cut(s)	259
Eco47I	GGWCC	1 cut(s)	290
Eco57I	CTGAAG	1 cut(s)	464
Eco72I	CACGTG	1 cut(s)	702
EcoRI	GAATTC	1 cut(s)	572
EcoRII	CCWGG	3 cut(s)	292, 588, 598
EcoRV	GATATC	1 cut(s)	259
EcoT14I	CCWWGG	1 cut(s)	400
ErhI	CCWWGG	1 cut(s)	400
FaeI	CATG	5 cut(s)	404, 739, 763, 794, 1025
FatI	CATG	5 cut(s)	400, 735, 759, 790, 1021
FauI	CCCGC	1 cut(s)	587
FblI	GTMKAC	1 cut(s)	525
Fnu4HI	GCNGC	1 cut(s)	330
FokI	GGATG	3 cut(s)	158, 677, 940
Fsp4HI	GCNGC	1 cut(s)	330
FspBI	CTAG	2 cut(s)	225, 356
GlaI	GCGC	1 cut(s)	550
GluI	GCNGC	1 cut(s)	330
HaeII	RGCGCY	1 cut(s)	552
HaeIII	GGCC	3 cut(s)	204, 265, 515
HapII	CCGG	3 cut(s)	124, 583, 662
HgaI	GACGC	1 cut(s)	733
HhaI	GCGC	1 cut(s)	551
Hin1II	CATG	5 cut(s)	404, 739, 763, 794, 1025
Hin6I	GCGC	1 cut(s)	549
HinP1I	GCGC	1 cut(s)	549
HindIII	AAGCTT	2 cut(s)	518, 959
HinfI	GANTC	9 cut(s)	4, 83, 153, 228, 236, 376, 605, 814, 830
HpaII	CCGG	3 cut(s)	124, 583, 662
HphI	GGTGA	2 cut(s)	640, 839
Hpy166II	GTNNAC	2 cut(s)	526, 631
Hpy188III	TCNNGA	6 cut(s)	116, 310, 323, 677, 709, 971
Hpy8I	GTNNAC	2 cut(s)	526, 631
Hpy99I	CGWCG	2 cut(s)	81, 353
HpyAV	CCTTC	2 cut(s)	160, 221
HpyCH4III	ACNGT	2 cut(s)	277, 839
HpyCH4IV	ACGT	2 cut(s)	633, 701
HpyCH4V	TGCA	6 cut(s)	192, 410, 479, 769, 782, 890
HpyF10VI	GCNNNNNNNGC	1 cut(s)	361
HpyF3I	CTNAG	3 cut(s)	67, 333, 923
HpySE526I	ACGT	2 cut(s)	633, 701
Hsp92II	CATG	5 cut(s)	404, 739, 763, 794, 1025
HspAI	GCGC	1 cut(s)	549
KroI	GCCGGC	1 cut(s)	582
KroNI	GCCGGC	1 cut(s)	584
Kzo9I	GATC	4 cut(s)	112, 285, 466, 1045
LmnI	GCTCC	3 cut(s)	257, 536, 591
Lsp1109I	GCAGC	1 cut(s)	316
LweI	GCATC	1 cut(s)	877
MaeI	CTAG	2 cut(s)	225, 356
MaeII	ACGT	2 cut(s)	633, 701
MaeIII	GTNAC	1 cut(s)	978
MalI	GATC	4 cut(s)	114, 287, 468, 1047
MboI	GATC	4 cut(s)	112, 285, 466, 1045
MflI	RGATCY	1 cut(s)	1045
MlsI	TGGCCA	1 cut(s)	515
MluCI	AATT	6 cut(s)	40, 572, 764, 912, 918, 1011
MluNI	TGGCCA	1 cut(s)	515
MlyI	GAGTC	1 cut(s)	230
MmeI	TCCRAC	1 cut(s)	100
MnlI	CCTC	8 cut(s)	125, 135, 151, 242, 468, 516, 780, 799
Mox20I	TGGCCA	1 cut(s)	515
MroNI	GCCGGC	1 cut(s)	582
MscI	TGGCCA	1 cut(s)	515
MseI	TTAA	3 cut(s)	636, 669, 999
Msp20I	TGGCCA	1 cut(s)	515
MspI	CCGG	3 cut(s)	124, 583, 662
MspR9I	CCNGG	3 cut(s)	294, 590, 600
Mva1269I	GAATGC	1 cut(s)	689
MvaI	CCWGG	3 cut(s)	294, 590, 600
MwoI	GCNNNNNNNGC	1 cut(s)	361
NaeI	GCCGGC	1 cut(s)	584
NcoI	CCATGG	1 cut(s)	400
NdeII	GATC	4 cut(s)	112, 285, 466, 1045
NgoMIV	GCCGGC	1 cut(s)	582
NlaIII	CATG	5 cut(s)	404, 739, 763, 794, 1025
NlaIV	GGNNCC	3 cut(s)	532, 587, 733
NspV	TTCGAA	3 cut(s)	247, 544, 939
PcsI	WCGNNNNNNNCGW	1 cut(s)	348
PctI	GAATGC	1 cut(s)	689
PdiI	GCCGGC	1 cut(s)	584
PfeI	GAWTC	8 cut(s)	4, 83, 153, 228, 376, 605, 814, 830
PfoI	TCCNGGA	1 cut(s)	598
PkrI	GCNGC	1 cut(s)	331
PleI	GAGTC	1 cut(s)	230
PmaCI	CACGTG	1 cut(s)	702
PmlI	CACGTG	1 cut(s)	702
PpsI	GAGTC	1 cut(s)	230
Ppu21I	YACGTR	1 cut(s)	702
Psp1406I	AACGTT	1 cut(s)	633
Psp6I	CCWGG	3 cut(s)	292, 588, 598
PspCI	CACGTG	1 cut(s)	702
PspGI	CCWGG	3 cut(s)	292, 588, 598
PspN4I	GGNNCC	3 cut(s)	532, 587, 733
PspPI	GGNCC	1 cut(s)	290
PstI	CTGCAG	1 cut(s)	784
PsuI	RGATCY	1 cut(s)	1045
RsaI	GTAC	1 cut(s)	56
RsaNI	GTAC	1 cut(s)	55
SaqAI	TTAA	3 cut(s)	636, 669, 999
SatI	GCNGC	1 cut(s)	330
Sau3AI	GATC	4 cut(s)	112, 285, 466, 1045
Sau96I	GGNCC	1 cut(s)	290
SchI	GAGTC	1 cut(s)	230
ScrFI	CCNGG	3 cut(s)	294, 590, 600
SfaNI	GCATC	1 cut(s)	877
SfcI	CTRYAG	1 cut(s)	780
SfuI	TTCGAA	3 cut(s)	247, 544, 939
SinI	GGWCC	1 cut(s)	290
Sse9I	AATT	6 cut(s)	40, 572, 764, 912, 918, 1011
SsiI	CCGC	3 cut(s)	121, 580, 626
SspI	AATATT	1 cut(s)	484
SspMI	CTAG	2 cut(s)	225, 356
StyD4I	CCNGG	3 cut(s)	292, 588, 598
StyI	CCWWGG	1 cut(s)	400
TaaI	ACNGT	2 cut(s)	277, 839
TaiI	ACGT	2 cut(s)	636, 704
TaqI	TCGA	8 cut(s)	10, 156, 234, 247, 351, 544, 797, 939
TasI	AATT	6 cut(s)	40, 572, 764, 912, 918, 1011
TfiI	GAWTC	8 cut(s)	4, 83, 153, 228, 376, 605, 814, 830
Tru1I	TTAA	3 cut(s)	636, 669, 999
Tru9I	TTAA	3 cut(s)	636, 669, 999
TscAI	CASTG	2 cut(s)	304, 844
TseI	GCWGC	1 cut(s)	329
TspDTI	ATGAA	2 cut(s)	329, 827
TspRI	CASTG	2 cut(s)	304, 844
VpaK11BI	GGWCC	1 cut(s)	290
XapI	RAATTY	3 cut(s)	572, 918, 1011
XmiI	GTMKAC	1 cut(s)	525
XspI	CTAG	2 cut(s)	225, 356

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.