AT5G66420

Uncharacterised conserved protein UCP033271 (InterPro IPR008322), TIM-barrel signal transduction protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0008344
Physical Location & Seq
Reverse (-)
26521674 .. 26525177
3504 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G66420.3

Sequence Viewer

Length: 1968 bp
ATGAGCAAATGTCTTGAGAATTTTCTTAGGCAAGCCTTTGAGGATAATTCTCTTGCTGGGGCTATAGGCCTTGGCGGAAGCGGAGGAACATCGCTCATATCATCTGCTTTTAGGTCTCTCCCTATTGGGATTCCTAAGGTCATTGTATCCACTGTGGCCAGTGGTCAAACTGAACCTTATGTTGGGACCTCAGATTTGGTTTTGATCCCTTCTTTAGTAGATGTTTGTGGTATCAACAGTGTTAGCAGGGTGGTCTTTTCAAACGCTGGGGCTTCTTTTGCTGGTATGGTAGTCGGGAGGCTTGAGTTGTTTAGAAGCTCCCCAAGCGATAACGGCAAATGTACTGTGGGCATAACTATGTTTGGAGTGACAACTCCATGTGTAAATGCAGTCCAGGAGATATTAACAAGAGAAGGTTATGAGACACTAGTGTTTCATGCCACTGGTGTTGGTGGCAGAGCAATGGAATCTTTGGTTAAAGAAGGATTTATACAGGGAGTAATGGATATTACAACAACCGAGGTTGCCGACCATCTCGTTGGAGGGGTGATGGCATGCGATAGTTCCCGTTTTGATATCACCATAGAAAAAGGAATACCGTTGGTTTTAAGTGTAGGTGCCTTAGATATGGTGAACTTTGGTGGTAAAGATACAATACCTTCTCATTTCCAAACGAGAAAGATTCACGTCCACAACGAACAGGTTTCTCTCATCCGAACGACAGCAGAAGAGAATAAGAAATTTGCAAGGTTTATAGCAGATAAACTGAACAAATCAACATCAAAAGTAAGGGTTTGCATTCCAGAGAAAGGTTTATCTGCACTGGATGCTCCAGGGAAGCCTTTCTGTGATCCAGAAGCTACTGGTGCTCTCATAAATGAATTACAGGGGCTCATTCAAACTAATGATGACAGACAAGTCAATATTTATTCTCACCACATCAATGACCCTGAGTTTGCAGAGGCATTGGTTGCTTCATTTCTTGAGATTTGTCCAAAAACTTACGCACAAATCAAACCCTCTGAAACGACTTCGACTAAACCTAGTACTGGAGAGCATGATGATGGACATGTGAGATCGAGGCTTGAAAGGATTCCATACAGTCCTAAAGAGTTTCCCAATGCAAAGCCAGAAACCTTGGAACGAACTCAGGCCATTTTGGGACGGTTGAGAGATCAAATAGAGAAGGGAATACCAATAATTGGGGGAGGTGCTGGTACTGGAATATCTGCAAAGTTTGAGGAAGCTGGTGGGATTGATTTGATAGTGATATACAACTCTGGACGTTTTCGTATGGCTGGAAGAGGATCCTTAGCAGGCTTACTTCCATTTGCTGATGCCAATGCAGTCGTGCTTGAAATGGCAAATGAAGTTTTACCTGTAGTGAAGGCGGTGCCTGTTCTGGCTGGGGTGTGCGCAACAGATCCATTTCGTCGTATGGACTATTTCCTGAAGCAGTTGGAGTCCATTGGGTTCGTTGGTGTCCAGAACTTTCCAACTGTTGGTCTCTTTGATGGTAATTTTAGACAAAATCTTGAGGAGACAGGAATGGGATATGGTCTTGAAGTTAAAATGATCTCAGAAGCGCACAAAATGGGGCTGTTGACCACTCCATATGCTTTCAACCCAAAAGAAGGAGAAGAAATGGCAAAAGCGGGAGCTGATATCATAGTAGCCCACATGGGTCTAACGACATCCGGAAATATTGGGGCGAAAACCGCAGTTTCAGTGGAAGAAAGCGTTGTTCGTGTACAAGCTATTGCAGATGCTGCTCGTAGATTCAACCCAGACATTATCGTCCTCTGCCACGGAGGTCCGATATCGGGTCCAGAAGAGGCAGAGTTTGTGTTGAAGAGAACACAGGGTTGTGTCCATGGCTTCTACGGAGCATCAAGCATGGAAAGGCTACCTGTAGAACAAGCAATAACAAACACTGTTCAAAAATACAAGTCCATATCGATCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PEP_hydrolase UPF0261

Protein Analysis

655

Amino Acids

70.03

Weight (kDa)

5.74

Isoelectric Point (pI)

34.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UPF0261 PF06792 7 - 198 2.3e-54 Uncharacterised protein family (UPF0261) N-terminal domain
UPF0261_C PF23189 212 - 429 2.7e-94 Uncharacterised protein family (UPF0261) C-terminal domain
PEP_hydrolase PF09370 485 - 618 7e-67 Phosphoenolpyruvate hydrolase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 917, 1796
Acc16I TGCGCA 1 cut(s) 1417
AccB1I GGYRCC 2 cut(s) 617, 1393
AccB7I CCANNNNNTGG 2 cut(s) 1202, 1502
AccIII TCCGGA 1 cut(s) 1697
AciI CCGC 5 cut(s) 75, 81, 1391, 1655, 1719
AclWI GGATC 5 cut(s) 199, 845, 1302, 1315, 1418
AcoI YGGCCR 1 cut(s) 156
AcsI RAATTY 2 cut(s) 19, 740
AcuI CTGAAG 1 cut(s) 1472
AfaI GTAC 4 cut(s) 343, 1048, 1219, 1752
AfiI CCNNNNNNNGG 7 cut(s) 182, 809, 1049, 1202, 1502, 1634, 1823
AflIII ACRYGT 1 cut(s) 1069
AgsI TTSAA 9 cut(s) 261, 899, 1088, 1358, 1565, 1624, 1783, 1852, 1940
AhlI ACTAGT 1 cut(s) 427
AjiI CACGTC 1 cut(s) 688
AjnI CCWGG 2 cut(s) 393, 832
AjuI GAANNNNNNNTTGG 2 cut(s) 165, 197
AluBI AGCT 5 cut(s) 318, 860, 1247, 1661, 1757
AluI AGCT 5 cut(s) 318, 860, 1247, 1661, 1757
Alw21I GWGCWC 1 cut(s) 871
Alw26I GTCTC 4 cut(s) 120, 416, 1511, 1535
AlwI GGATC 5 cut(s) 199, 845, 1302, 1315, 1418
AlwNI CAGNNNCTG 1 cut(s) 1769
Aor13HI TCCGGA 1 cut(s) 1697
AoxI GGCC 3 cut(s) 67, 156, 1152
ApeKI GCWGC 1 cut(s) 1769
ApoI RAATTY 2 cut(s) 19, 740
ArsI GACNNNNNNTTYG 4 cut(s) 178, 210, 938, 970
Asp700I GAANNNNTTC 2 cut(s) 842, 1092
AspLEI GCGC 2 cut(s) 1418, 1588
AspS9I GGNCC 3 cut(s) 186, 1814, 1826
AsuHPI GGTGA 4 cut(s) 559, 571, 643, 926
AvaII GGWCC 3 cut(s) 186, 1814, 1826
AxyI CCTNAGG 1 cut(s) 135
BalI TGGCCA 1 cut(s) 158
BamHI GGATCC 1 cut(s) 1307
BanI GGYRCC 2 cut(s) 617, 1393
BanII GRGCYC 1 cut(s) 894
BarI GAAGNNNNNNTAC 2 cut(s) 474, 506
Bbv12I GWGCWC 1 cut(s) 871
BbvI GCAGC 1 cut(s) 1756
BccI CCATC 4 cut(s) 540, 544, 1058, 1508
BceAI ACGGC 1 cut(s) 349
BciT130I CCWGG 2 cut(s) 395, 834
BciVI GTATCC 1 cut(s) 157
BcoDI GTCTC 4 cut(s) 120, 416, 1511, 1535
BcuI ACTAGT 1 cut(s) 427
BfaI CTAG 2 cut(s) 428, 1044
BfmI CTRYAG 3 cut(s) 63, 1380, 1911
BfuI GTATCC 1 cut(s) 157
BisI GCNGC 1 cut(s) 1770
BlsI GCNGC 1 cut(s) 1771
BmcAI AGTACT 1 cut(s) 1048
Bme1390I CCNGG 2 cut(s) 395, 834
Bme18I GGWCC 3 cut(s) 186, 1814, 1826
BmgBI CACGTC 1 cut(s) 688
BmgT120I GGNCC 3 cut(s) 186, 1814, 1826
BmiI GGNNCC 5 cut(s) 187, 619, 1309, 1395, 1827
BmrFI CCNGG 2 cut(s) 395, 834
BmsI GCATC 4 cut(s) 817, 1327, 1756, 1898
BpmI CTGGAG 2 cut(s) 816, 1071
Bpu10I CCTNAGC 1 cut(s) 1312
BpuEI CTTGAG 4 cut(s) 35, 323, 1004, 1556
Bsa29I ATCGAT 1 cut(s) 1958
BsaI GGTCTC 2 cut(s) 120, 1511
BsaJI CCNNGG 6 cut(s) 70, 519, 833, 1137, 1807, 1873
BsaWI WCCGGW 1 cut(s) 1697
Bsc4I CCNNNNNNNGG 7 cut(s) 182, 809, 1049, 1202, 1502, 1634, 1823
Bse1I ACTGG 6 cut(s) 159, 448, 828, 868, 1054, 1225
Bse21I CCTNAGG 1 cut(s) 135
Bse3DI GCAATG 1 cut(s) 468
BseAI TCCGGA 1 cut(s) 1697
BseBI CCWGG 2 cut(s) 395, 834
BseCI ATCGAT 1 cut(s) 1958
BseDI CCNNGG 6 cut(s) 70, 519, 833, 1137, 1807, 1873
BseGI GGATG 3 cut(s) 711, 832, 1694
BseLI CCNNNNNNNGG 7 cut(s) 182, 809, 1049, 1202, 1502, 1634, 1823
BseMI GCAATG 1 cut(s) 468
BseMII CTCAG 4 cut(s) 204, 942, 1163, 1593
BseNI ACTGG 6 cut(s) 159, 448, 828, 868, 1054, 1225
BseRI GAGGAG 1 cut(s) 1553
BseXI GCAGC 1 cut(s) 1756
BseYI CCCAGC 3 cut(s) 56, 266, 1406
BsgI GTGCAG 1 cut(s) 804
BshFI GGCC 3 cut(s) 69, 158, 1154
BshNI GGYRCC 2 cut(s) 617, 1393
BshVI ATCGAT 1 cut(s) 1958
BsiHKAI GWGCWC 1 cut(s) 871
BsiSI CCGG 1 cut(s) 1698
BslFI GGGAC 2 cut(s) 199, 1176
BslI CCNNNNNNNGG 7 cut(s) 182, 809, 1049, 1202, 1502, 1634, 1823
BsmAI GTCTC 4 cut(s) 120, 416, 1511, 1535
BsmFI GGGAC 2 cut(s) 199, 1176
BsmI GAATGC 1 cut(s) 798
BsnI GGCC 3 cut(s) 69, 158, 1154
Bso31I GGTCTC 2 cut(s) 120, 1511
Bsp1286I GDGCHC 2 cut(s) 871, 894
Bsp13I TCCGGA 1 cut(s) 1697
Bsp1407I TGTACA 1 cut(s) 1750
Bsp143I GATC 8 cut(s) 204, 850, 1076, 1174, 1307, 1423, 1575, 1959
Bsp19I CCATGG 1 cut(s) 1873
BspACI CCGC 5 cut(s) 75, 81, 1391, 1655, 1719
BspANI GGCC 3 cut(s) 69, 158, 1154
BspCNI CTCAG 4 cut(s) 203, 943, 1162, 1592
BspDI ATCGAT 1 cut(s) 1958
BspEI TCCGGA 1 cut(s) 1697
BspLI GGNNCC 5 cut(s) 187, 619, 1309, 1395, 1827
BspPI GGATC 5 cut(s) 199, 845, 1302, 1315, 1418
BspT107I GGYRCC 2 cut(s) 617, 1393
BspTNI GGTCTC 2 cut(s) 120, 1511
BsrDI GCAATG 1 cut(s) 468
BsrGI TGTACA 1 cut(s) 1750
BsrI ACTGG 6 cut(s) 159, 448, 828, 868, 1054, 1225
BssECI CCNNGG 6 cut(s) 70, 519, 833, 1137, 1807, 1873
BssMI GATC 8 cut(s) 204, 850, 1076, 1174, 1307, 1423, 1575, 1959
BssT1I CCWWGG 3 cut(s) 70, 1137, 1873
Bst2UI CCWGG 2 cut(s) 395, 834
Bst4CI ACNGT 8 cut(s) 154, 239, 346, 600, 1103, 1167, 1501, 1936
Bst6I CTCTTC 4 cut(s) 723, 1297, 1827, 1847
BstAPI GCANNNNNTGC 3 cut(s) 827, 971, 1769
BstAUI TGTACA 1 cut(s) 1750
BstC8I GCNNGC 3 cut(s) 33, 556, 1318
BstDEI CTNAG 8 cut(s) 26, 135, 190, 622, 951, 1149, 1312, 1579
BstDSI CCRYGG 2 cut(s) 1807, 1873
BstF5I GGATG 3 cut(s) 711, 832, 1694
BstHHI GCGC 2 cut(s) 1418, 1588
BstKTI GATC 8 cut(s) 207, 853, 1079, 1177, 1310, 1426, 1578, 1962
BstMAI GTCTC 4 cut(s) 120, 416, 1511, 1535
BstMBI GATC 8 cut(s) 204, 850, 1076, 1174, 1307, 1423, 1575, 1959
BstMWI GCNNNNNNNGC 8 cut(s) 278, 324, 333, 827, 866, 971, 1718, 1769
BstNI CCWGG 2 cut(s) 395, 834
BstNSI RCATGY 2 cut(s) 558, 1073
BstSCI CCNGG 2 cut(s) 393, 832
BstSFI CTRYAG 3 cut(s) 63, 1380, 1911
BstV1I GCAGC 1 cut(s) 1756
BstX2I RGATCY 2 cut(s) 1307, 1423
BstXI CCANNNNNNTGG 1 cut(s) 539
BstYI RGATCY 2 cut(s) 1307, 1423
Bsu15I ATCGAT 1 cut(s) 1958
Bsu36I CCTNAGG 1 cut(s) 135
BsuI GTATCC 1 cut(s) 157
BsuRI GGCC 3 cut(s) 69, 158, 1154
BsuTUI ATCGAT 1 cut(s) 1958
BtgI CCRYGG 2 cut(s) 1807, 1873
BtgZI GCGATG 1 cut(s) 75
BtrI CACGTC 1 cut(s) 688
BtsCI GGATG 3 cut(s) 711, 832, 1694
BtsIMutI CAGTG 7 cut(s) 150, 166, 244, 441, 821, 1734, 1932
Cac8I GCNNGC 3 cut(s) 33, 556, 1318
CaiI CAGNNNCTG 1 cut(s) 1769
CfoI GCGC 2 cut(s) 1418, 1588
Cfr13I GGNCC 3 cut(s) 186, 1814, 1826
ClaI ATCGAT 1 cut(s) 1958
Csp6I GTAC 4 cut(s) 342, 1047, 1218, 1751
CspCI CAANNNNNGTGG 2 cut(s) 430, 465
CviAII CATG 8 cut(s) 378, 437, 555, 1058, 1070, 1681, 1874, 1897
CviQI GTAC 4 cut(s) 342, 1047, 1218, 1751
DdeI CTNAG 8 cut(s) 26, 135, 190, 622, 951, 1149, 1312, 1579
DpnI GATC 8 cut(s) 206, 852, 1078, 1176, 1309, 1425, 1577, 1961
DpnII GATC 8 cut(s) 204, 850, 1076, 1174, 1307, 1423, 1575, 1959
DrdI GACNNNNNNGTC 2 cut(s) 917, 1796
DseDI GACNNNNNNGTC 2 cut(s) 917, 1796
EaeI YGGCCR 1 cut(s) 156
Eam1104I CTCTTC 4 cut(s) 723, 1297, 1827, 1847
EarI CTCTTC 4 cut(s) 723, 1297, 1827, 1847
EciI GGCGGA 1 cut(s) 90
Eco130I CCWWGG 3 cut(s) 70, 1137, 1873
Eco147I AGGCCT 1 cut(s) 69
Eco24I GRGCYC 1 cut(s) 894
Eco31I GGTCTC 2 cut(s) 120, 1511
Eco32I GATATC 3 cut(s) 577, 1666, 1821
Eco47I GGWCC 3 cut(s) 186, 1814, 1826
Eco57I CTGAAG 1 cut(s) 1472
Eco81I CCTNAGG 1 cut(s) 135
EcoO109I RGGNCCY 1 cut(s) 186
EcoRII CCWGG 2 cut(s) 393, 832
EcoRV GATATC 3 cut(s) 577, 1666, 1821
EcoT14I CCWWGG 3 cut(s) 70, 1137, 1873
EcoT38I GRGCYC 1 cut(s) 894
ErhI CCWWGG 3 cut(s) 70, 1137, 1873
FaeI CATG 8 cut(s) 381, 440, 558, 1061, 1073, 1684, 1877, 1900
FaqI GGGAC 2 cut(s) 199, 1176
FatI CATG 8 cut(s) 377, 436, 554, 1057, 1069, 1680, 1873, 1896
FauI CCCGC 1 cut(s) 1648
FauNDI CATATG 1 cut(s) 1615
Fnu4HI GCNGC 1 cut(s) 1770
FokI GGATG 3 cut(s) 698, 839, 1681
FriOI GRGCYC 1 cut(s) 894
Fsp4HI GCNGC 1 cut(s) 1770
FspBI CTAG 2 cut(s) 428, 1044
FspI TGCGCA 1 cut(s) 1417
GlaI GCGC 2 cut(s) 1417, 1587
GluI GCNGC 1 cut(s) 1770
GsaI CCCAGC 3 cut(s) 60, 270, 1410
GsuI CTGGAG 2 cut(s) 816, 1071
HaeIII GGCC 3 cut(s) 69, 158, 1154
HapII CCGG 1 cut(s) 1698
HhaI GCGC 2 cut(s) 1418, 1588
Hin1II CATG 8 cut(s) 381, 440, 558, 1061, 1073, 1684, 1877, 1900
Hin6I GCGC 2 cut(s) 1416, 1586
HinP1I GCGC 2 cut(s) 1416, 1586
HincII GTYRAC 1 cut(s) 1605
HindII GTYRAC 1 cut(s) 1605
HinfI GANTC 6 cut(s) 130, 467, 682, 1093, 1463, 1779
HpaII CCGG 1 cut(s) 1698
HphI GGTGA 4 cut(s) 559, 571, 643, 926
Hpy166II GTNNAC 4 cut(s) 634, 691, 1605, 1751
Hpy188I TCNGA 5 cut(s) 193, 716, 1024, 1582, 1818
Hpy8I GTNNAC 4 cut(s) 634, 691, 1605, 1751
Hpy99I CGWCG 1 cut(s) 1437
HpyAV CCTTC 7 cut(s) 219, 407, 476, 669, 1180, 1381, 1628
HpyCH4III ACNGT 8 cut(s) 154, 239, 346, 600, 1103, 1167, 1501, 1936
HpyCH4IV ACGT 2 cut(s) 687, 1285
HpyCH4V TGCA 9 cut(s) 389, 746, 798, 821, 959, 1124, 1232, 1346, 1763
HpyF10VI GCNNNNNNNGC 8 cut(s) 278, 324, 333, 827, 866, 971, 1718, 1769
HpyF3I CTNAG 8 cut(s) 26, 135, 190, 622, 951, 1149, 1312, 1579
HpySE526I ACGT 2 cut(s) 687, 1285
Hsp92II CATG 8 cut(s) 381, 440, 558, 1061, 1073, 1684, 1877, 1900
HspAI GCGC 2 cut(s) 1416, 1586
Kpn2I TCCGGA 1 cut(s) 1697
Kzo9I GATC 8 cut(s) 204, 850, 1076, 1174, 1307, 1423, 1575, 1959
LmnI GCTCC 4 cut(s) 323, 835, 1658, 1886
Lsp1109I GCAGC 1 cut(s) 1756
LweI GCATC 4 cut(s) 817, 1327, 1756, 1898
MaeI CTAG 2 cut(s) 428, 1044
MaeII ACGT 2 cut(s) 687, 1285
MaeIII GTNAC 1 cut(s) 367
MalI GATC 8 cut(s) 206, 852, 1078, 1176, 1309, 1425, 1577, 1961
MboI GATC 8 cut(s) 204, 850, 1076, 1174, 1307, 1423, 1575, 1959
MboII GAAGA 6 cut(s) 740, 1314, 1652, 1745, 1844, 1864
MflI RGATCY 2 cut(s) 1307, 1423
MhlI GDGCHC 2 cut(s) 871, 894
MlsI TGGCCA 1 cut(s) 158
MluCI AATT 6 cut(s) 19, 46, 740, 881, 1200, 1519
MluNI TGGCCA 1 cut(s) 158
MlyI GAGTC 1 cut(s) 1472
MmeI TCCRAC 3 cut(s) 520, 1440, 1520
Mox20I TGGCCA 1 cut(s) 158
MroI TCCGGA 1 cut(s) 1697
MroXI GAANNNNTTC 2 cut(s) 842, 1092
MscI TGGCCA 1 cut(s) 158
MseI TTAA 4 cut(s) 404, 477, 608, 1569
MslI CAYNNNNRTG 3 cut(s) 356, 942, 1062
Msp20I TGGCCA 1 cut(s) 158
MspI CCGG 1 cut(s) 1698
MspR9I CCNGG 2 cut(s) 395, 834
Mva1269I GAATGC 1 cut(s) 798
MvaI CCWGG 2 cut(s) 395, 834
MwoI GCNNNNNNNGC 8 cut(s) 278, 324, 333, 827, 866, 971, 1718, 1769
NcoI CCATGG 1 cut(s) 1873
NdeI CATATG 1 cut(s) 1615
NdeII GATC 8 cut(s) 204, 850, 1076, 1174, 1307, 1423, 1575, 1959
NlaIII CATG 8 cut(s) 381, 440, 558, 1061, 1073, 1684, 1877, 1900
NlaIV GGNNCC 5 cut(s) 187, 619, 1309, 1395, 1827
NmuCI GTSAC 1 cut(s) 367
NsbI TGCGCA 1 cut(s) 1417
NspI RCATGY 2 cut(s) 558, 1073
PaeI GCATGC 1 cut(s) 558
PceI AGGCCT 1 cut(s) 69
PciI ACATGT 1 cut(s) 1069
PcsI WCGNNNNNNNCGW 1 cut(s) 693
PctI GAATGC 1 cut(s) 798
PdmI GAANNNNTTC 2 cut(s) 842, 1092
PfeI GAWTC 5 cut(s) 130, 467, 682, 1093, 1779
PflMI CCANNNNNTGG 2 cut(s) 1202, 1502
PfoI TCCNGGA 1 cut(s) 393
PkrI GCNGC 1 cut(s) 1771
PleI GAGTC 1 cut(s) 1471
PpsI GAGTC 1 cut(s) 1471
PpuMI RGGWCCY 1 cut(s) 186
PscI ACATGT 1 cut(s) 1069
Psp5II RGGWCCY 1 cut(s) 186
Psp6I CCWGG 2 cut(s) 393, 832
PspFI CCCAGC 3 cut(s) 56, 266, 1406
PspGI CCWGG 2 cut(s) 393, 832
PspN4I GGNNCC 5 cut(s) 187, 619, 1309, 1395, 1827
PspPI GGNCC 3 cut(s) 186, 1814, 1826
PspPPI RGGWCCY 1 cut(s) 186
PstNI CAGNNNCTG 1 cut(s) 1769
PsuI RGATCY 2 cut(s) 1307, 1423
RsaI GTAC 4 cut(s) 343, 1048, 1219, 1752
RsaNI GTAC 4 cut(s) 342, 1047, 1218, 1751
RseI CAYNNNNRTG 3 cut(s) 356, 942, 1062
SaqAI TTAA 4 cut(s) 404, 477, 608, 1569
SatI GCNGC 1 cut(s) 1770
Sau3AI GATC 8 cut(s) 204, 850, 1076, 1174, 1307, 1423, 1575, 1959
Sau96I GGNCC 3 cut(s) 186, 1814, 1826
ScaI AGTACT 1 cut(s) 1048
SchI GAGTC 1 cut(s) 1472
ScrFI CCNGG 2 cut(s) 395, 834
SduI GDGCHC 2 cut(s) 871, 894
SfaNI GCATC 4 cut(s) 817, 1327, 1756, 1898
SfcI CTRYAG 3 cut(s) 63, 1380, 1911
SinI GGWCC 3 cut(s) 186, 1814, 1826
SmiMI CAYNNNNRTG 3 cut(s) 356, 942, 1062
SmlI CTYRAG 4 cut(s) 14, 302, 983, 1535
SmoI CTYRAG 4 cut(s) 14, 302, 983, 1535
SpeI ACTAGT 1 cut(s) 427
SphI GCATGC 1 cut(s) 558
Sse9I AATT 6 cut(s) 19, 46, 740, 881, 1200, 1519
SseBI AGGCCT 1 cut(s) 69
SsiI CCGC 5 cut(s) 75, 81, 1391, 1655, 1719
SspI AATATT 2 cut(s) 925, 1705
SspMI CTAG 2 cut(s) 428, 1044
StuI AGGCCT 1 cut(s) 69
StyD4I CCNGG 2 cut(s) 393, 832
StyI CCWWGG 3 cut(s) 70, 1137, 1873
TaaI ACNGT 8 cut(s) 154, 239, 346, 600, 1103, 1167, 1501, 1936
TaiI ACGT 2 cut(s) 690, 1288
TaqI TCGA 3 cut(s) 1034, 1079, 1958
TasI AATT 6 cut(s) 19, 46, 740, 881, 1200, 1519
TatI WGTACW 3 cut(s) 341, 1046, 1750
TfiI GAWTC 5 cut(s) 130, 467, 682, 1093, 1779
Tru1I TTAA 4 cut(s) 404, 477, 608, 1569
Tru9I TTAA 4 cut(s) 404, 477, 608, 1569
TscAI CASTG 7 cut(s) 157, 166, 244, 448, 828, 1734, 1939
TseFI GTSAC 1 cut(s) 367
TseI GCWGC 1 cut(s) 1769
Tsp45I GTSAC 1 cut(s) 367
TspDTI ATGAA 4 cut(s) 425, 894, 966, 1383
TspGWI ACGGA 2 cut(s) 1824, 1899
TspRI CASTG 7 cut(s) 157, 166, 244, 448, 828, 1734, 1939
Van91I CCANNNNNTGG 2 cut(s) 1202, 1502
VpaK11BI GGWCC 3 cut(s) 186, 1814, 1826
XapI RAATTY 2 cut(s) 19, 740
XceI RCATGY 2 cut(s) 558, 1073
XmnI GAANNNNTTC 2 cut(s) 842, 1092
XspI CTAG 2 cut(s) 428, 1044
ZrmI AGTACT 1 cut(s) 1048
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.