MD02G1142900.v1.1

Uncharacterised protein family (UPF0261)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0008344
Physical Location & Seq
Reverse (-)
11883827 .. 11884489
663 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1142900.v1.1.491

Sequence Viewer

Length: 453 bp
ATGCCGGCCCATAAAATCCCCCGGGTTTTCTGTATCGGAACCTCCGATACGAAGCTTACGATCTCCGATTCCTCGCCGAGTCGGTCCGATCCCATCTCGATTCCTTCTCCGCAGCTTCCTCACTCAAGATTCTCAATGTCGATACCGAAACTACAGGTCAAAGTCTTGGTGGTGGACGTATCGGCGAGTCGGACCGAGACCGATACAAATCCAACCGATTTCGAATTCGTTTCGAGAAAGGAGATTCTCCGTCACTATTCTGAAGCAGCAGCCGATCAGCTTCCGGTGGACAGAGGCGAAGCCGTCGCTGTAATGAGCAAAGAGCTCGAGAACTTCCTAACCCAAGCCCAAAAGGACGGCGTTTTAGCCGGAGCTGTTGGTCTCGGAGGCAGCGAAGGCACGGCACTGATATCCTCCGCCCTAAGATCTCTCCAATCGGAGTGCCGAAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PEP_hydrolase UPF0261

Protein Analysis

151

Amino Acids

16.1

Weight (kDa)

5.9

Isoelectric Point (pI)

62.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UPF0261 PF06792 48 - 145 9.9e-16 Uncharacterised protein family (UPF0261) N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 110, 417
AclWI GGATC 1 cut(s) 83
AcsI RAATTY 1 cut(s) 224
AcuI CTGAAG 1 cut(s) 282
AluBI AGCT 6 cut(s) 55, 115, 280, 325, 374, 450
AluI AGCT 6 cut(s) 55, 115, 280, 325, 374, 450
Alw21I GWGCWC 1 cut(s) 327
Alw26I GTCTC 2 cut(s) 191, 386
AlwI GGATC 1 cut(s) 83
Ama87I CYCGRG 2 cut(s) 21, 326
AoxI GGCC 1 cut(s) 6
ApeKI GCWGC 4 cut(s) 112, 266, 269, 390
ApoI RAATTY 1 cut(s) 224
AspS9I GGNCC 3 cut(s) 7, 84, 192
AsuC2I CCSGG 2 cut(s) 22, 23
AsuII TTCGAA 1 cut(s) 222
AvaI CYCGRG 2 cut(s) 21, 326
AvaII GGWCC 2 cut(s) 84, 192
BanII GRGCYC 1 cut(s) 327
Bbv12I GWGCWC 1 cut(s) 327
BbvI GCAGC 4 cut(s) 124, 278, 281, 402
BccI CCATC 1 cut(s) 101
BceAI ACGGC 3 cut(s) 287, 373, 417
BcgI CGANNNNNNTGC 2 cut(s) 307, 341
BcnI CCSGG 2 cut(s) 22, 23
BcoDI GTCTC 2 cut(s) 191, 386
BfmI CTRYAG 1 cut(s) 152
BglII AGATCT 1 cut(s) 425
BisI GCNGC 4 cut(s) 113, 267, 270, 391
BlsI GCNGC 4 cut(s) 114, 268, 271, 392
Bme1390I CCNGG 2 cut(s) 22, 23
Bme18I GGWCC 2 cut(s) 84, 192
BmeT110I CYCGRG 2 cut(s) 21, 326
BmgT120I GGNCC 3 cut(s) 7, 84, 192
BmiI GGNNCC 1 cut(s) 40
BmrFI CCNGG 2 cut(s) 22, 23
Bpu14I TTCGAA 1 cut(s) 222
BpuEI CTTGAG 1 cut(s) 109
BpuMI CCSGG 2 cut(s) 22, 23
BsaBI GATNNNNATC 1 cut(s) 207
BsaI GGTCTC 2 cut(s) 191, 386
BsaJI CCNNGG 2 cut(s) 20, 21
BsaWI WCCGGW 1 cut(s) 283
Bse118I RCCGGY 1 cut(s) 4
Bse8I GATNNNNATC 1 cut(s) 207
BseDI CCNNGG 2 cut(s) 20, 21
BseJI GATNNNNATC 1 cut(s) 207
BseXI GCAGC 4 cut(s) 124, 278, 281, 402
BshFI GGCC 1 cut(s) 8
BsiHKAI GWGCWC 1 cut(s) 327
BsiHKCI CYCGRG 2 cut(s) 21, 326
BsiSI CCGG 4 cut(s) 5, 22, 284, 369
BsmAI GTCTC 2 cut(s) 191, 386
BsnI GGCC 1 cut(s) 8
Bso31I GGTCTC 2 cut(s) 191, 386
BsoBI CYCGRG 2 cut(s) 21, 326
Bsp119I TTCGAA 1 cut(s) 222
Bsp1286I GDGCHC 1 cut(s) 327
Bsp143I GATC 4 cut(s) 60, 88, 274, 425
BspACI CCGC 2 cut(s) 110, 417
BspANI GGCC 1 cut(s) 8
BspLI GGNNCC 1 cut(s) 40
BspPI GGATC 1 cut(s) 83
BspT104I TTCGAA 1 cut(s) 222
BspTNI GGTCTC 2 cut(s) 191, 386
BsrFI RCCGGY 1 cut(s) 4
BssAI RCCGGY 1 cut(s) 4
BssECI CCNNGG 2 cut(s) 20, 21
BssMI GATC 4 cut(s) 60, 88, 274, 425
BstBI TTCGAA 1 cut(s) 222
BstC8I GCNNGC 1 cut(s) 6
BstDEI CTNAG 1 cut(s) 422
BstKTI GATC 4 cut(s) 63, 91, 277, 428
BstMAI GTCTC 2 cut(s) 191, 386
BstMBI GATC 4 cut(s) 60, 88, 274, 425
BstMWI GCNNNNNNNGC 1 cut(s) 396
BstSCI CCNGG 2 cut(s) 20, 21
BstSFI CTRYAG 1 cut(s) 152
BstV1I GCAGC 4 cut(s) 124, 278, 281, 402
BstX2I RGATCY 1 cut(s) 425
BstYI RGATCY 1 cut(s) 425
BsuRI GGCC 1 cut(s) 8
BtsIMutI CAGTG 1 cut(s) 404
Cac8I GCNNGC 1 cut(s) 6
Cfr10I RCCGGY 1 cut(s) 4
Cfr13I GGNCC 3 cut(s) 7, 84, 192
Cfr9I CCCGGG 1 cut(s) 21
CpoI CGGWCCG 2 cut(s) 84, 192
CspI CGGWCCG 2 cut(s) 84, 192
DdeI CTNAG 1 cut(s) 422
DpnI GATC 4 cut(s) 62, 90, 276, 427
DpnII GATC 4 cut(s) 60, 88, 274, 425
EciI GGCGGA 1 cut(s) 406
Ecl136II GAGCTC 1 cut(s) 325
Eco24I GRGCYC 1 cut(s) 327
Eco31I GGTCTC 2 cut(s) 191, 386
Eco32I GATATC 1 cut(s) 411
Eco47I GGWCC 2 cut(s) 84, 192
Eco53kI GAGCTC 1 cut(s) 325
Eco57I CTGAAG 1 cut(s) 282
Eco88I CYCGRG 2 cut(s) 21, 326
EcoICRI GAGCTC 1 cut(s) 325
EcoRI GAATTC 1 cut(s) 224
EcoRV GATATC 1 cut(s) 411
EcoT38I GRGCYC 1 cut(s) 327
FaiI YATR 1 cut(s) 12
Fnu4HI GCNGC 4 cut(s) 113, 267, 270, 391
FriOI GRGCYC 1 cut(s) 327
Fsp4HI GCNGC 4 cut(s) 113, 267, 270, 391
GluI GCNGC 4 cut(s) 113, 267, 270, 391
HaeIII GGCC 1 cut(s) 8
HapII CCGG 4 cut(s) 5, 22, 284, 369
HindIII AAGCTT 1 cut(s) 53
HinfI GANTC 6 cut(s) 68, 79, 100, 129, 187, 244
HpaII CCGG 4 cut(s) 5, 22, 284, 369
Hpy166II GTNNAC 2 cut(s) 175, 289
Hpy188I TCNGA 8 cut(s) 38, 46, 67, 88, 192, 262, 386, 439
Hpy188III TCNNGA 4 cut(s) 97, 126, 234, 328
Hpy8I GTNNAC 2 cut(s) 175, 289
Hpy99I CGWCG 1 cut(s) 308
HpyAV CCTTC 2 cut(s) 114, 389
HpyCH4IV ACGT 1 cut(s) 177
HpyF10VI GCNNNNNNNGC 1 cut(s) 396
HpyF3I CTNAG 1 cut(s) 422
HpySE526I ACGT 1 cut(s) 177
KroI GCCGGC 1 cut(s) 4
KroNI GCCGGC 1 cut(s) 6
Kzo9I GATC 4 cut(s) 60, 88, 274, 425
LmnI GCTCC 1 cut(s) 371
LpnPI CCDG 5 cut(s) 18, 35, 140, 297, 382
Lsp1109I GCAGC 4 cut(s) 124, 278, 281, 402
MaeII ACGT 1 cut(s) 177
MaeIII GTNAC 1 cut(s) 251
MalI GATC 4 cut(s) 62, 90, 276, 427
MboI GATC 4 cut(s) 60, 88, 274, 425
MflI RGATCY 1 cut(s) 425
MhlI GDGCHC 1 cut(s) 327
MluCI AATT 1 cut(s) 224
MlyI GAGTC 2 cut(s) 88, 196
MmeI TCCRAC 2 cut(s) 170, 236
MnlI CCTC 6 cut(s) 52, 82, 129, 287, 380, 424
MroNI GCCGGC 1 cut(s) 4
MspI CCGG 4 cut(s) 5, 22, 284, 369
MspR9I CCNGG 2 cut(s) 22, 23
MwoI GCNNNNNNNGC 1 cut(s) 396
NaeI GCCGGC 1 cut(s) 6
NciI CCSGG 2 cut(s) 22, 23
NdeII GATC 4 cut(s) 60, 88, 274, 425
NgoMIV GCCGGC 1 cut(s) 4
NlaIV GGNNCC 1 cut(s) 40
NmeAIII GCCGAG 1 cut(s) 102
NmuCI GTSAC 1 cut(s) 251
NspV TTCGAA 1 cut(s) 222
PaeR7I CTCGAG 1 cut(s) 326
PcsI WCGNNNNNNNCGW 3 cut(s) 42, 56, 390
PdiI GCCGGC 1 cut(s) 6
PfeI GAWTC 4 cut(s) 68, 100, 129, 244
PkrI GCNGC 4 cut(s) 114, 268, 271, 392
PleI GAGTC 2 cut(s) 87, 195
PpsI GAGTC 2 cut(s) 87, 195
Psp124BI GAGCTC 1 cut(s) 327
PspN4I GGNNCC 1 cut(s) 40
PspPI GGNCC 3 cut(s) 7, 84, 192
PsrI GAACNNNNNNTAC 2 cut(s) 31, 63
PsuI RGATCY 1 cut(s) 425
Rsr2I CGGWCCG 2 cut(s) 84, 192
RsrII CGGWCCG 2 cut(s) 84, 192
SacI GAGCTC 1 cut(s) 327
SatI GCNGC 4 cut(s) 113, 267, 270, 391
Sau3AI GATC 4 cut(s) 60, 88, 274, 425
Sau96I GGNCC 3 cut(s) 7, 84, 192
SchI GAGTC 2 cut(s) 88, 196
ScrFI CCNGG 2 cut(s) 22, 23
SduI GDGCHC 1 cut(s) 327
SetI ASST 9 cut(s) 44, 57, 117, 159, 180, 282, 327, 376, 452
SfcI CTRYAG 1 cut(s) 152
Sfr274I CTCGAG 1 cut(s) 326
SfuI TTCGAA 1 cut(s) 222
SinI GGWCC 2 cut(s) 84, 192
SlaI CTCGAG 1 cut(s) 326
SmaI CCCGGG 1 cut(s) 23
SmlI CTYRAG 2 cut(s) 124, 326
SmoI CTYRAG 2 cut(s) 124, 326
Sse9I AATT 1 cut(s) 224
SsiI CCGC 2 cut(s) 110, 417
SstI GAGCTC 1 cut(s) 327
StyD4I CCNGG 2 cut(s) 20, 21
TaiI ACGT 1 cut(s) 180
TaqI TCGA 5 cut(s) 98, 140, 222, 233, 327
TaqII GACCGA 3 cut(s) 72, 209, 215
TasI AATT 1 cut(s) 224
TfiI GAWTC 4 cut(s) 68, 100, 129, 244
TscAI CASTG 1 cut(s) 411
TseFI GTSAC 1 cut(s) 251
TseI GCWGC 4 cut(s) 112, 266, 269, 390
Tsp45I GTSAC 1 cut(s) 251
TspGWI ACGGA 1 cut(s) 239
TspMI CCCGGG 1 cut(s) 21
TspRI CASTG 1 cut(s) 411
VpaK11BI GGWCC 2 cut(s) 84, 192
XapI RAATTY 1 cut(s) 224
XhoI CTCGAG 1 cut(s) 326
XmaI CCCGGG 1 cut(s) 21
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.