Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0008344

Physical Location & Seq

Genomic Coordinates Reverse (-)

11880941 .. 11882734

Gene Structure 1794 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1142700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 912 bp

ATGAAGATCGGAAGGTACAATTATTTCCCATTGAATGAAACCTTGCAAGGAACTAGAGTGGTATTGCAGCAACTCAAAGATCAAATAAACAGAGGAATTCCAATTATAGGGGCAGGTGCAGGGACAGGCATATCTGCAAAGTTTGAAGAAGCTGGTGGTTTTGATCTGATGGTTGTTTACAATTCAGGGCGCTTTTGCGTGGCAGGAAGAGGTTCGTTGGCAGGTTTGTTACCTTCTGCTGATGCAAATGCTGTAGTACTTGACATGGCCAATGAAGTTTTGCCAGTAGGTTTCAACCCTTTCGTAATTTGTATGCTGGAAGATTGTTTTAGTATAATCCAAAATATTCAAGTAATCAAATTCTTAGTGGTGAAGAAGGTACCTGTTCTTGCTGGGGTATGTGGCACCGATCCTTTTTGCCTTATGGATTTCTTCCTAAGGCAGGTGGAGTCGATTGGATTCTCTGGTGTCCAAAAATTTCCAACAGTTGGACTCTATGATGGTAAATTTAGACAAAATCTTGAAGAAACTGGAATGGGATACCTATTGGAGGTTGAGATGATTGAAAAAGCACATAAGATGGGGCTCTTGACAACCCCATATGCTTTCAATCAAGATGAAGCTATGCAAATGGCGAAAGCTGGTGCTGATATTATCGTGGCTCATATGGGAATGGGACTCACTACATCCGGCTCTATAGGGGCAAGGACGGCTGTTTCACCGGAGGAAAGTGTAGTTCATGTACAAAATATTGCAGATGCTGCAAGTAGGATCAATCCCAACGCCATTGTGCTTTGCCATGGTGGTATGCGCTCGGGAGCTTTTCATTTCTCGTTTCCAAGTTTCCTTTTTATCACATTAATGAACATGAAGATTGTTTGTACCATACTTGACCAATCCCGAAACTACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PEP_hydrolase UPF0261

Protein Analysis

304

Amino Acids

32.83

Weight (kDa)

5.97

Isoelectric Point (pI)

36.98

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PEP_hydrolase	PF09370	21 - 102	3.9e-29	Phosphoenolpyruvate hydrolase-like
PEP_hydrolase	PF09370	123 - 269	2.4e-63	Phosphoenolpyruvate hydrolase-like

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008344)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G66420 AT5G66420
fragaria_vesca	FvH4_1g12760 FvH4_1g28681
malus_domestica	MD02G1142700.v1.1 MD02G1142900.v1.1 MD15G1256300.v1.1
prunus_persica	Prupe.7G158600_v2.0.a1 Prupe.7G158600_v2.0.a1
pyrus_communis	pycom02g11270 pycom15g22420
rosa_chinensis	RchiOBHm_Chr2g0100861
rosa_laevigata	RLG00000017019
rosa_multiflora	Rmu_sc0001659.1_g000032 Rmu_sc0004947.1_g000028
rosa_roxburghii	Rroxscaffold_2G00141790
rosa_rugosa	Rorug02G0093300
rosa_samantha	Rh2AG140800 Rh2BG145900 Rh2CG147100 Rh2DG146500 Rh3DG359200
rosa_wichuraiana	Rw2G011040

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	104, 433
Acc36I	ACCTGC	3 cut(s)	104, 212, 433
Acc65I	GGTACC	1 cut(s)	379
AccB1I	GGYRCC	2 cut(s)	379, 404
AccB7I	CCANNNNNTGG	1 cut(s)	488
AclWI	GGATC	2 cut(s)	404, 779
AcoI	YGGCCR	1 cut(s)	267
AcsI	RAATTY	4 cut(s)	96, 359, 476, 506
AfaI	GTAC	5 cut(s)	17, 258, 381, 744, 883
AfiI	CCNNNNNNNGG	4 cut(s)	107, 442, 488, 550
AgsI	TTSAA	7 cut(s)	34, 146, 295, 350, 524, 566, 610
AluBI	AGCT	4 cut(s)	152, 623, 641, 821
AluI	AGCT	4 cut(s)	152, 623, 641, 821
AlwI	GGATC	2 cut(s)	404, 779
AlwNI	CAGNNNCTG	1 cut(s)	761
Ama87I	CYCGRG	1 cut(s)	814
AoxI	GGCC	1 cut(s)	267
ApeKI	GCWGC	2 cut(s)	67, 761
ApoI	RAATTY	4 cut(s)	96, 359, 476, 506
AseI	ATTAAT	1 cut(s)	860
Asp700I	GAANNNNTTC	1 cut(s)	211
Asp718I	GGTACC	1 cut(s)	379
AspLEI	GCGC	2 cut(s)	192, 813
AsuHPI	GGTGA	2 cut(s)	382, 711
AvaI	CYCGRG	1 cut(s)	814
AxyI	CCTNAGG	1 cut(s)	437
BalI	TGGCCA	1 cut(s)	269
BanI	GGYRCC	2 cut(s)	379, 404
BanII	GRGCYC	1 cut(s)	588
BbvI	GCAGC	2 cut(s)	79, 748
BccI	CCATC	3 cut(s)	163, 494, 574
BceAI	ACGGC	1 cut(s)	726
BciVI	GTATCC	1 cut(s)	533
BfaI	CTAG	1 cut(s)	54
BfmI	CTRYAG	2 cut(s)	252, 696
BfoI	RGCGCY	1 cut(s)	193
BfuAI	ACCTGC	3 cut(s)	104, 212, 433
BfuI	GTATCC	1 cut(s)	533
BisI	GCNGC	2 cut(s)	68, 762
BlsI	GCNGC	2 cut(s)	69, 763
BmcAI	AGTACT	1 cut(s)	258
BmeT110I	CYCGRG	1 cut(s)	814
BmiI	GGNNCC	2 cut(s)	381, 406
BmsI	GCATC	2 cut(s)	232, 748
BsaJI	CCNNGG	1 cut(s)	799
BsaWI	WCCGGW	1 cut(s)	721
Bsc4I	CCNNNNNNNGG	4 cut(s)	107, 442, 488, 550
Bse1I	ACTGG	2 cut(s)	284, 535
Bse21I	CCTNAGG	1 cut(s)	437
BseDI	CCNNGG	1 cut(s)	799
BseGI	GGATG	1 cut(s)	686
BseLI	CCNNNNNNNGG	4 cut(s)	107, 442, 488, 550
BseNI	ACTGG	2 cut(s)	284, 535
BseXI	GCAGC	2 cut(s)	79, 748
BseYI	CCCAGC	1 cut(s)	392
BsgI	GTGCAG	1 cut(s)	138
BshFI	GGCC	1 cut(s)	269
BshNI	GGYRCC	2 cut(s)	379, 404
BsiHKCI	CYCGRG	1 cut(s)	814
BsiSI	CCGG	2 cut(s)	690, 722
BslFI	GGGAC	2 cut(s)	136, 690
BslI	CCNNNNNNNGG	4 cut(s)	107, 442, 488, 550
BsmFI	GGGAC	2 cut(s)	136, 690
BsnI	GGCC	1 cut(s)	269
BsoBI	CYCGRG	1 cut(s)	814
Bsp1286I	GDGCHC	1 cut(s)	588
Bsp1407I	TGTACA	1 cut(s)	742
Bsp143I	GATC	5 cut(s)	6, 79, 163, 409, 771
Bsp19I	CCATGG	1 cut(s)	799
BspANI	GGCC	1 cut(s)	269
BspLI	GGNNCC	2 cut(s)	381, 406
BspMI	ACCTGC	3 cut(s)	104, 212, 433
BspPI	GGATC	2 cut(s)	404, 779
BspT107I	GGYRCC	2 cut(s)	379, 404
BsrGI	TGTACA	1 cut(s)	742
BsrI	ACTGG	2 cut(s)	284, 535
BssECI	CCNNGG	1 cut(s)	799
BssMI	GATC	5 cut(s)	6, 79, 163, 409, 771
BssT1I	CCWWGG	1 cut(s)	799
Bst4CI	ACNGT	1 cut(s)	487
Bst6I	CTCTTC	1 cut(s)	202
BstAPI	GCANNNNNTGC	1 cut(s)	761
BstAUI	TGTACA	1 cut(s)	742
BstDEI	CTNAG	2 cut(s)	364, 437
BstDSI	CCRYGG	1 cut(s)	799
BstENI	CCTNNNNNAGG	2 cut(s)	440, 548
BstF5I	GGATG	1 cut(s)	686
BstH2I	RGCGCY	1 cut(s)	193
BstHHI	GCGC	2 cut(s)	192, 813
BstKTI	GATC	5 cut(s)	9, 82, 166, 412, 774
BstMBI	GATC	5 cut(s)	6, 79, 163, 409, 771
BstMWI	GCNNNNNNNGC	2 cut(s)	710, 761
BstSFI	CTRYAG	2 cut(s)	252, 696
BstV1I	GCAGC	2 cut(s)	79, 748
Bsu36I	CCTNAGG	1 cut(s)	437
BsuI	GTATCC	1 cut(s)	533
BsuRI	GGCC	1 cut(s)	269
BtgI	CCRYGG	1 cut(s)	799
BtsCI	GGATG	1 cut(s)	686
BveI	ACCTGC	3 cut(s)	104, 212, 433
CaiI	CAGNNNCTG	1 cut(s)	761
CfoI	GCGC	2 cut(s)	192, 813
Csp6I	GTAC	5 cut(s)	16, 257, 380, 743, 882
CviAII	CATG	4 cut(s)	265, 740, 800, 868
CviJI	RGCY	9 cut(s)	152, 269, 586, 623, 641, 662, 693, 713, 821
CviKI_1	RGCY	9 cut(s)	152, 269, 586, 623, 641, 662, 693, 713, 821
CviQI	GTAC	5 cut(s)	16, 257, 380, 743, 882
DdeI	CTNAG	2 cut(s)	364, 437
DpnI	GATC	5 cut(s)	8, 81, 165, 411, 773
DpnII	GATC	5 cut(s)	6, 79, 163, 409, 771
EaeI	YGGCCR	1 cut(s)	267
Eam1104I	CTCTTC	1 cut(s)	202
EarI	CTCTTC	1 cut(s)	202
Eco130I	CCWWGG	1 cut(s)	799
Eco24I	GRGCYC	1 cut(s)	588
Eco81I	CCTNAGG	1 cut(s)	437
Eco88I	CYCGRG	1 cut(s)	814
EcoNI	CCTNNNNNAGG	2 cut(s)	440, 548
EcoRI	GAATTC	1 cut(s)	96
EcoT14I	CCWWGG	1 cut(s)	799
EcoT38I	GRGCYC	1 cut(s)	588
ErhI	CCWWGG	1 cut(s)	799
FaeI	CATG	4 cut(s)	268, 743, 803, 871
FaqI	GGGAC	2 cut(s)	136, 690
FatI	CATG	4 cut(s)	264, 739, 799, 867
FauNDI	CATATG	2 cut(s)	601, 666
Fnu4HI	GCNGC	2 cut(s)	68, 762
FokI	GGATG	1 cut(s)	673
FriOI	GRGCYC	1 cut(s)	588
Fsp4HI	GCNGC	2 cut(s)	68, 762
FspBI	CTAG	1 cut(s)	54
GlaI	GCGC	2 cut(s)	191, 812
GluI	GCNGC	2 cut(s)	68, 762
GsaI	CCCAGC	1 cut(s)	396
HaeII	RGCGCY	1 cut(s)	193
HaeIII	GGCC	1 cut(s)	269
HapII	CCGG	2 cut(s)	690, 722
HhaI	GCGC	2 cut(s)	192, 813
Hin1II	CATG	4 cut(s)	268, 743, 803, 871
Hin6I	GCGC	2 cut(s)	190, 811
HinP1I	GCGC	2 cut(s)	190, 811
HinfI	GANTC	4 cut(s)	449, 459, 492, 678
HpaII	CCGG	2 cut(s)	690, 722
HphI	GGTGA	2 cut(s)	382, 711
Hpy166II	GTNNAC	1 cut(s)	178
Hpy188I	TCNGA	2 cut(s)	11, 168
Hpy188III	TCNNGA	5 cut(s)	521, 589, 614, 816, 900
Hpy8I	GTNNAC	1 cut(s)	178
HpyAV	CCTTC	3 cut(s)	6, 243, 370
HpyCH4III	ACNGT	1 cut(s)	487
HpyCH4V	TGCA	8 cut(s)	46, 67, 119, 137, 245, 628, 755, 764
HpyF10VI	GCNNNNNNNGC	2 cut(s)	710, 761
HpyF3I	CTNAG	2 cut(s)	364, 437
Hsp92II	CATG	4 cut(s)	268, 743, 803, 871
HspAI	GCGC	2 cut(s)	190, 811
KpnI	GGTACC	1 cut(s)	383
Kzo9I	GATC	5 cut(s)	6, 79, 163, 409, 771
LmnI	GCTCC	1 cut(s)	818
Lsp1109I	GCAGC	2 cut(s)	79, 748
LweI	GCATC	2 cut(s)	232, 748
MaeI	CTAG	1 cut(s)	54
MaeIII	GTNAC	1 cut(s)	228
MalI	GATC	5 cut(s)	8, 81, 165, 411, 773
MboI	GATC	5 cut(s)	6, 79, 163, 409, 771
MboII	GAAGA	8 cut(s)	16, 158, 219, 332, 385, 424, 536, 883
MhlI	GDGCHC	1 cut(s)	588
MlsI	TGGCCA	1 cut(s)	269
MluCI	AATT	8 cut(s)	19, 96, 102, 181, 306, 359, 476, 506
MluNI	TGGCCA	1 cut(s)	269
MlyI	GAGTC	3 cut(s)	458, 486, 672
MmeI	TCCRAC	2 cut(s)	469, 506
MnlI	CCTC	4 cut(s)	86, 203, 544, 718
Mox20I	TGGCCA	1 cut(s)	269
MroXI	GAANNNNTTC	1 cut(s)	211
MscI	TGGCCA	1 cut(s)	269
MseI	TTAA	1 cut(s)	860
MslI	CAYNNNNRTG	1 cut(s)	860
Msp20I	TGGCCA	1 cut(s)	269
MspI	CCGG	2 cut(s)	690, 722
MwoI	GCNNNNNNNGC	2 cut(s)	710, 761
NcoI	CCATGG	1 cut(s)	799
NdeI	CATATG	2 cut(s)	601, 666
NdeII	GATC	5 cut(s)	6, 79, 163, 409, 771
NlaIII	CATG	4 cut(s)	268, 743, 803, 871
NlaIV	GGNNCC	2 cut(s)	381, 406
PaqCI	CACCTGC	2 cut(s)	104, 433
PdmI	GAANNNNTTC	1 cut(s)	211
PfeI	GAWTC	1 cut(s)	459
PflMI	CCANNNNNTGG	1 cut(s)	488
PkrI	GCNGC	2 cut(s)	69, 763
PleI	GAGTC	3 cut(s)	457, 486, 672
PpsI	GAGTC	3 cut(s)	457, 486, 672
PshBI	ATTAAT	1 cut(s)	860
PspFI	CCCAGC	1 cut(s)	392
PspN4I	GGNNCC	2 cut(s)	381, 406
PstNI	CAGNNNCTG	1 cut(s)	761
RsaI	GTAC	5 cut(s)	17, 258, 381, 744, 883
RsaNI	GTAC	5 cut(s)	16, 257, 380, 743, 882
RseI	CAYNNNNRTG	1 cut(s)	860
SaqAI	TTAA	1 cut(s)	860
SatI	GCNGC	2 cut(s)	68, 762
Sau3AI	GATC	5 cut(s)	6, 79, 163, 409, 771
ScaI	AGTACT	1 cut(s)	258
SchI	GAGTC	3 cut(s)	458, 486, 672
SduI	GDGCHC	1 cut(s)	588
SfaNI	GCATC	2 cut(s)	232, 748
SfcI	CTRYAG	2 cut(s)	252, 696
SmiMI	CAYNNNNRTG	1 cut(s)	860
Sse9I	AATT	8 cut(s)	19, 96, 102, 181, 306, 359, 476, 506
SspI	AATATT	2 cut(s)	346, 751
SspMI	CTAG	1 cut(s)	54
StyI	CCWWGG	1 cut(s)	799
TaaI	ACNGT	1 cut(s)	487
TaqI	TCGA	1 cut(s)	452
TasI	AATT	8 cut(s)	19, 96, 102, 181, 306, 359, 476, 506
TatI	WGTACW	2 cut(s)	256, 742
TfiI	GAWTC	1 cut(s)	459
Tru1I	TTAA	1 cut(s)	860
Tru9I	TTAA	1 cut(s)	860
TseI	GCWGC	2 cut(s)	67, 761
TspDTI	ATGAA	8 cut(s)	17, 51, 288, 633, 728, 815, 878, 884
Van91I	CCANNNNNTGG	1 cut(s)	488
VspI	ATTAAT	1 cut(s)	860
XagI	CCTNNNNNAGG	2 cut(s)	440, 548
XapI	RAATTY	4 cut(s)	96, 359, 476, 506
XmnI	GAANNNNTTC	1 cut(s)	211
XspI	CTAG	1 cut(s)	54
ZrmI	AGTACT	1 cut(s)	258

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.