Rroxscaffold_2G00141790

Uncharacterised protein family (UPF0261)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0008344
Physical Location & Seq
Forward (+)
80034023 .. 80038573
4551 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00141790.1

Sequence Viewer

Length: 2259 bp
ATGCCGGCGGACGATAAAATTCGCCGGGTTTTCTGTATCGGAACCGCCGATACGAAGCTCGAGGAGCTCCGATTCCTCGCCGACTCAGTCCGATCCAACCTCGCCGCATTCGCCACATCTTCCTCATCCAAGGTTCAAGTCACCGTTGTGGATGTCTCCGTCAGCCGGAACCACTCCGATTCCGATCATAACATAACCGATTTCGCCTTTGTTTCGAGAAACGAGGTGCTTCGTAGCAACTCCGATGCCCCGGACCAGCTTCCGGAGGATAGAGGCGAAGCGGTGGATGTGATGAGCAAGGCGCTGGAGATTTTCGTAACGAAATCGCAAAACGACGGCGTTTTAGTTGGAGCGATAGGCGTCGGAGGCAGCGGAGGCACGGCTCTGGTATCCCCGGCGCTTAGATCGCTTCCGATCGGAGTGCCGAAGCTTATTGTGTCCACCGTGGCGAGCGGCCGGACGGACCATTACGTAGGGACCTCGGATTTGGTGCTGTACCCGTCGATTGTGGACGTGTGTGGGATTAATAGCGTGAGCAGGGTCGTATTGAACAATGCTGCGGCTGCATTTGGAGGGATGGTGATTGGAAGACTTGAGAGGAAAGAGAGTGGTGGTGAGGGTGAGAAGTCAACGGTGGGGTTGACCATGTTTGGGGTTACCACTACTTGTGTGAATGCTGTGAAAGAGAGACTGGAGGAGGAGGGGTATGAGACTCTGGTTTTTCATGCTACTGGGGTTGGTGGTAGGGCCATGGAATCTCTGGTAAAGGAGGGGTTTATAAAGGGTGTTTTGGATATCACAACAACAGAAGTTGCGGACTATGTGGTTGGAGGTGTCATGGCTTGTGACCCTTCTCGCTTTGATGCTACTGTAGAGAAAGGGGTTCCTTTAGTCCTGAGCGTTGGAGCTTTGGATATGGTAAACTTTGGAGCTAAAGATACCATACCATCCAATTTTCAAAAAAGAAAGATTCATGAACATAATAAGCAGGTTTCACTGATGAGAACCACAGTAGATGAAATCAAAATATTTGCTAGCTTCGTAGCAGATAAACTGAACAAGTCATCATCAAAGGTCGTTGTTTGCCTGCCGCAAAAGGGTGTCTCTGCTTTGGATGCACCAGGGATGGCCTTTTATGATCCTGAGGCTACCGCTACTCTGATAAATGAACTTAAGAGGCTCATTAAGACAAATGAAGATCGGCAGGTAAAGGTGTACCCTCATCATATAAATGACCCTGAGTTTGCAAATGCATTGGTTGACTCATTTTTAGAGATTAGTATGAAGAGTCCTCTTCAGTCTACTCTGCAAGTTGCTTCTCCCGAGCCCAGTCAAGAAGTTCATGAAAGTTCTGTTGCCCAGATAAACTTCTCAAGCTGTGAGACCACTCCACGCAGCCTAAGTGACTTCCCAGATGCAAAACCAGAAACTCTGCAAAGAACTTGGGCAATACTACAGCAACTAAGAGATCAAATAAATAAAGGAATACCAATTATAGGGGCTGGGGCAGGGACGGGTATATCCGCAAAGTTTGAGGAAGCTGGTGGAGTTGACCTGATAGTGTTGTACAATTCAGGGCGCTTTCGCATGGCAGGAAGAGGTTCCTTGGCAGGCTTATTGCCTTTTGCTGATGCAAATGCTGTAGTACTTGACATGGCTAATGAAGTTTTACCAGTGGTGAAGAAGGTACCGGTTCTTGCCGGAGTATGTGGGACTGATCCTTTTCGCCGAATGGATTTCTTCCTGAGGCAGGTGGAGTCAATTGGATTCTTTGGAGTTCAAAATTTTCCAACTGTTGGATTATTTGATGGTAATTTTAGACAAAATCTTGAAGAAACTGGAATGGGATATCGATTGGAGGTTGATATGATTGAAAAAGCACATAAGATGGGTCTCTTGACAACCCCATATGCTTTCAATCAAGATGAAGCTGTGGAAATGGCAAAAGGTGGTGCTGATATTATAGTGGCTCATATGGGACTCACTACAGCTGGCTCTATAGGGGCAAAGACAGCTGTTTCGCTGGAGGAAAGTATAGTTCGTGTACAAACTATTGCAGATGCTGCACATAGGATCAATCCCAATGCTATTGTGCTCTGCCATGGTGGTCCTATCTCTAGCCCAAAAGAGGCAGAATTTGTACTTAAGAACACCAAGGGAGTTCATGGCTTTTATGGTGCATCGAGCATGGAGAGGCTACCCGTTGAACAAGCTATAACCTCCACAATGCAACAGTACAAATCAATCTCTTTCCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PEP_hydrolase UPF0261

Protein Analysis

752

Amino Acids

80.63

Weight (kDa)

5.47

Isoelectric Point (pI)

38.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UPF0261 PF06792 9 - 195 3.9e-54 Uncharacterised protein family (UPF0261) N-terminal domain
UPF0261_C PF23189 209 - 426 1.7e-94 Uncharacterised protein family (UPF0261) C-terminal domain
PEP_hydrolase PF09370 484 - 749 2.7e-130 Phosphoenolpyruvate hydrolase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 779
AarI CACCTGC 1 cut(s) 1741
Acc36I ACCTGC 3 cut(s) 979, 1195, 1741
Acc65I GGTACC 1 cut(s) 1687
AccB1I GGYRCC 1 cut(s) 1687
AccB7I CCANNNNNTGG 1 cut(s) 1796
AccBSI CCGCTC 1 cut(s) 453
AccI GTMKAC 1 cut(s) 1301
AccIII TCCGGA 1 cut(s) 262
AclWI GGATC 4 cut(s) 87, 1133, 1712, 2081
AcoI YGGCCR 1 cut(s) 454
AcsI RAATTY 3 cut(s) 18, 1783, 2135
AcuI CTGAAG 1 cut(s) 1280
AcyI GRCGYC 1 cut(s) 360
AfaI GTAC 8 cut(s) 497, 1217, 1568, 1647, 1689, 2046, 2142, 2237
AfiI CCNNNNNNNGG 8 cut(s) 165, 262, 651, 1097, 1496, 1750, 1796, 2128
AflII CTTAAG 2 cut(s) 1172, 2144
AflIII ACRYGT 1 cut(s) 513
AgeI ACCGGT 1 cut(s) 1690
AgsI TTSAA 8 cut(s) 137, 550, 959, 1781, 1832, 1874, 1918, 2207
AjiI CACGTC 1 cut(s) 514
AjnI CCWGG 1 cut(s) 1120
AleI CACNNNNGTG 1 cut(s) 146
Alw21I GWGCWC 2 cut(s) 69, 2097
Alw26I GTCTC 6 cut(s) 160, 682, 704, 1108, 1376, 1898
AlwI GGATC 4 cut(s) 87, 1133, 1712, 2081
AlwNI CAGNNNCTG 1 cut(s) 2063
Ama87I CYCGRG 2 cut(s) 59, 1322
Aor13HI TCCGGA 1 cut(s) 262
AoxI GGCC 3 cut(s) 454, 747, 1128
ApeKI GCWGC 5 cut(s) 369, 557, 563, 1395, 2063
ApoI RAATTY 3 cut(s) 18, 1783, 2135
ArsI GACNNNNNNTTYG 6 cut(s) 469, 501, 1226, 1258, 2002, 2034
AseI ATTAAT 1 cut(s) 525
AsiGI ACCGGT 1 cut(s) 1690
Asp700I GAANNNNTTC 1 cut(s) 1600
Asp718I GGTACC 1 cut(s) 1687
AspLEI GCGC 3 cut(s) 304, 400, 1581
AspS9I GGNCC 5 cut(s) 253, 463, 477, 747, 2108
AsuC2I CCSGG 3 cut(s) 26, 251, 395
AsuHPI GGTGA 5 cut(s) 133, 592, 626, 632, 1690
AsuNHI GCTAGC 1 cut(s) 1034
AvaI CYCGRG 2 cut(s) 59, 1322
AvaII GGWCC 4 cut(s) 253, 463, 477, 2108
AxyI CCTNAGG 2 cut(s) 1143, 1745
BanI GGYRCC 1 cut(s) 1687
BanII GRGCYC 2 cut(s) 69, 1329
BbsI GAAGAC 1 cut(s) 595
Bbv12I GWGCWC 2 cut(s) 69, 2097
BbvI GCAGC 5 cut(s) 381, 544, 550, 1407, 2050
BccI CCATC 5 cut(s) 571, 955, 1120, 1802, 1882
BceAI ACGGC 2 cut(s) 352, 396
BcgI CGANNNNNNTGC 2 cut(s) 403, 437
BciT130I CCWGG 1 cut(s) 1122
BciVI GTATCC 1 cut(s) 400
BcnI CCSGG 3 cut(s) 26, 251, 395
BcoDI GTCTC 6 cut(s) 160, 682, 704, 1108, 1376, 1898
BfaI CTAG 2 cut(s) 1035, 2118
BfmI CTRYAG 5 cut(s) 870, 1454, 1641, 1986, 1998
BfoI RGCGCY 3 cut(s) 305, 401, 1582
BfrI CTTAAG 2 cut(s) 1172, 2144
BfuAI ACCTGC 3 cut(s) 979, 1195, 1741
BfuI GTATCC 1 cut(s) 400
BisI GCNGC 9 cut(s) 105, 370, 454, 558, 561, 564, 1091, 1396, 2064
BlsI GCNGC 9 cut(s) 106, 371, 455, 559, 562, 565, 1092, 1397, 2065
BmcAI AGTACT 1 cut(s) 1647
Bme1390I CCNGG 4 cut(s) 26, 251, 395, 1122
Bme18I GGWCC 4 cut(s) 253, 463, 477, 2108
BmeT110I CYCGRG 2 cut(s) 59, 1322
BmgBI CACGTC 1 cut(s) 514
BmgT120I GGNCC 5 cut(s) 253, 463, 477, 747, 2108
BmiI GGNNCC 6 cut(s) 43, 170, 478, 885, 1603, 1689
BmrFI CCNGG 4 cut(s) 26, 251, 395, 1122
BmrI ACTGGG 2 cut(s) 741, 1323
BmsI GCATC 7 cut(s) 235, 853, 1105, 1405, 1621, 2050, 2189
BmtI GCTAGC 1 cut(s) 1038
BmuI ACTGGG 2 cut(s) 741, 1323
BpiI GAAGAC 1 cut(s) 595
BpmI CTGGAG 3 cut(s) 326, 713, 2045
Bpu10I CCTNAGC 1 cut(s) 896
BpuEI CTTGAG 2 cut(s) 614, 1357
BpuMI CCSGG 3 cut(s) 26, 251, 395
Bsa29I ATCGAT 1 cut(s) 1852
BsaAI YACGTR 1 cut(s) 472
BsaBI GATNNNNATC 1 cut(s) 183
BsaHI GRCGYC 1 cut(s) 360
BsaI GGTCTC 2 cut(s) 1376, 1898
BsaWI WCCGGW 2 cut(s) 262, 1690
BsaXI ACNNNNNCTCC 4 cut(s) 564, 594, 689, 719
Bsc4I CCNNNNNNNGG 8 cut(s) 165, 262, 651, 1097, 1496, 1750, 1796, 2128
Bse118I RCCGGY 2 cut(s) 4, 1690
Bse1I ACTGG 5 cut(s) 696, 736, 1329, 1673, 1843
Bse21I CCTNAGG 2 cut(s) 1143, 1745
Bse8I GATNNNNATC 1 cut(s) 183
BseAI TCCGGA 1 cut(s) 262
BseBI CCWGG 1 cut(s) 1122
BseCI ATCGAT 1 cut(s) 1852
BseGI GGATG 7 cut(s) 125, 157, 292, 582, 947, 1120, 1131
BseJI GATNNNNATC 1 cut(s) 183
BseLI CCNNNNNNNGG 8 cut(s) 165, 262, 651, 1097, 1496, 1750, 1796, 2128
BseMII CTCAG 5 cut(s) 99, 887, 1134, 1230, 1736
BseNI ACTGG 5 cut(s) 696, 736, 1329, 1673, 1843
BseRI GAGGAG 3 cut(s) 77, 710, 713
BseX3I CGGCCG 1 cut(s) 454
BseXI GCAGC 5 cut(s) 381, 544, 550, 1407, 2050
BseYI CCCAGC 1 cut(s) 1502
BsgI GTGCAG 1 cut(s) 2049
Bsh1285I CGRYCG 2 cut(s) 417, 457
BshFI GGCC 3 cut(s) 456, 749, 1130
BshNI GGYRCC 1 cut(s) 1687
BshTI ACCGGT 1 cut(s) 1690
BshVI ATCGAT 1 cut(s) 1852
BsiEI CGRYCG 2 cut(s) 417, 457
BsiHKAI GWGCWC 2 cut(s) 69, 2097
BsiHKCI CYCGRG 2 cut(s) 59, 1322
BsiSI CCGG 9 cut(s) 5, 25, 166, 251, 263, 395, 457, 1691, 1701
BslFI GGGAC 4 cut(s) 490, 1525, 1726, 1992
BslI CCNNNNNNNGG 8 cut(s) 165, 262, 651, 1097, 1496, 1750, 1796, 2128
BsmAI GTCTC 6 cut(s) 160, 682, 704, 1108, 1376, 1898
BsmFI GGGAC 4 cut(s) 490, 1525, 1726, 1992
BsmI GAATGC 2 cut(s) 107, 679
BsnI GGCC 3 cut(s) 456, 749, 1130
Bso31I GGTCTC 2 cut(s) 1376, 1898
BsoBI CYCGRG 2 cut(s) 59, 1322
Bsp1286I GDGCHC 3 cut(s) 69, 1329, 2097
Bsp13I TCCGGA 1 cut(s) 262
Bsp1407I TGTACA 2 cut(s) 1566, 2044
Bsp143I GATC 9 cut(s) 92, 184, 404, 414, 1138, 1198, 1468, 1717, 2073
Bsp19I CCATGG 2 cut(s) 750, 2101
BspANI GGCC 3 cut(s) 456, 749, 1130
BspCNI CTCAG 5 cut(s) 98, 888, 1135, 1231, 1737
BspDI ATCGAT 1 cut(s) 1852
BspEI TCCGGA 1 cut(s) 262
BspHI TCATGA 2 cut(s) 973, 1342
BspLI GGNNCC 6 cut(s) 43, 170, 478, 885, 1603, 1689
BspMI ACCTGC 3 cut(s) 979, 1195, 1741
BspOI GCTAGC 1 cut(s) 1038
BspPI GGATC 4 cut(s) 87, 1133, 1712, 2081
BspT107I GGYRCC 1 cut(s) 1687
BspTI CTTAAG 2 cut(s) 1172, 2144
BspTNI GGTCTC 2 cut(s) 1376, 1898
BsrBI CCGCTC 1 cut(s) 453
BsrFI RCCGGY 2 cut(s) 4, 1690
BsrGI TGTACA 2 cut(s) 1566, 2044
BsrI ACTGG 5 cut(s) 696, 736, 1329, 1673, 1843
BssAI RCCGGY 2 cut(s) 4, 1690
BssMI GATC 9 cut(s) 92, 184, 404, 414, 1138, 1198, 1468, 1717, 2073
BssNI GRCGYC 1 cut(s) 360
BssT1I CCWWGG 5 cut(s) 129, 750, 1605, 2101, 2154
Bst2UI CCWGG 1 cut(s) 1122
Bst4CI ACNGT 7 cut(s) 145, 445, 634, 871, 1012, 1795, 2235
Bst6I CTCTTC 3 cut(s) 1280, 1299, 1591
BstACI GRCGYC 1 cut(s) 360
BstAFI CTTAAG 2 cut(s) 1172, 2144
BstAPI GCANNNNNTGC 1 cut(s) 2063
BstAUI TGTACA 2 cut(s) 1566, 2044
BstBAI YACGTR 1 cut(s) 472
BstC8I GCNNGC 6 cut(s) 6, 451, 1036, 1088, 1612, 1993
BstDEI CTNAG 8 cut(s) 85, 401, 896, 1143, 1239, 1400, 1463, 1745
BstDSI CCRYGG 3 cut(s) 444, 750, 2101
BstEII GGTNACC 1 cut(s) 655
BstENI CCTNNNNNAGG 1 cut(s) 1748
BstF5I GGATG 7 cut(s) 125, 157, 292, 582, 947, 1120, 1131
BstH2I RGCGCY 3 cut(s) 305, 401, 1582
BstHHI GCGC 3 cut(s) 304, 400, 1581
BstKTI GATC 9 cut(s) 95, 187, 407, 417, 1141, 1201, 1471, 1720, 2076
BstMAI GTCTC 6 cut(s) 160, 682, 704, 1108, 1376, 1898
BstMBI GATC 9 cut(s) 92, 184, 404, 414, 1138, 1198, 1468, 1717, 2073
BstMCI CGRYCG 2 cut(s) 417, 457
BstMWI GCNNNNNNNGC 9 cut(s) 64, 110, 366, 375, 406, 563, 1115, 2012, 2063
BstNI CCWGG 1 cut(s) 1122
BstPI GGTNACC 1 cut(s) 655
BstSCI CCNGG 4 cut(s) 24, 249, 393, 1120
BstSFI CTRYAG 5 cut(s) 870, 1454, 1641, 1986, 1998
BstSNI TACGTA 1 cut(s) 472
BstV1I GCAGC 5 cut(s) 381, 544, 550, 1407, 2050
BstV2I GAAGAC 1 cut(s) 595
BstZI CGGCCG 1 cut(s) 454
Bsu15I ATCGAT 1 cut(s) 1852
Bsu36I CCTNAGG 2 cut(s) 1143, 1745
BsuI GTATCC 1 cut(s) 400
BsuRI GGCC 3 cut(s) 456, 749, 1130
BsuTUI ATCGAT 1 cut(s) 1852
BtgI CCRYGG 3 cut(s) 444, 750, 2101
BtrI CACGTC 1 cut(s) 514
BtsCI GGATG 7 cut(s) 125, 157, 292, 582, 947, 1120, 1131
BtsIMutI CAGTG 3 cut(s) 995, 1680, 2254
BveI ACCTGC 3 cut(s) 979, 1195, 1741
Cac8I GCNNGC 6 cut(s) 6, 451, 1036, 1088, 1612, 1993
CaiI CAGNNNCTG 1 cut(s) 2063
CciI TCATGA 2 cut(s) 973, 1342
CfoI GCGC 3 cut(s) 304, 400, 1581
Cfr10I RCCGGY 2 cut(s) 4, 1690
Cfr13I GGNCC 5 cut(s) 253, 463, 477, 747, 2108
ClaI ATCGAT 1 cut(s) 1852
CseI GACGC 1 cut(s) 349
Csp6I GTAC 8 cut(s) 496, 1216, 1567, 1646, 1688, 2045, 2141, 2236
CspAI ACCGGT 1 cut(s) 1690
CviQI GTAC 8 cut(s) 496, 1216, 1567, 1646, 1688, 2045, 2141, 2236
DdeI CTNAG 8 cut(s) 85, 401, 896, 1143, 1239, 1400, 1463, 1745
DpnI GATC 9 cut(s) 94, 186, 406, 416, 1140, 1200, 1470, 1719, 2075
DpnII GATC 9 cut(s) 92, 184, 404, 414, 1138, 1198, 1468, 1717, 2073
EaeI YGGCCR 1 cut(s) 454
EagI CGGCCG 1 cut(s) 454
Eam1104I CTCTTC 3 cut(s) 1280, 1299, 1591
EarI CTCTTC 3 cut(s) 1280, 1299, 1591
EciI GGCGGA 1 cut(s) 23
Ecl136II GAGCTC 1 cut(s) 67
EclXI CGGCCG 1 cut(s) 454
Eco105I TACGTA 1 cut(s) 472
Eco130I CCWWGG 5 cut(s) 129, 750, 1605, 2101, 2154
Eco24I GRGCYC 2 cut(s) 69, 1329
Eco31I GGTCTC 2 cut(s) 1376, 1898
Eco32I GATATC 2 cut(s) 796, 1850
Eco47I GGWCC 4 cut(s) 253, 463, 477, 2108
Eco52I CGGCCG 1 cut(s) 454
Eco53kI GAGCTC 1 cut(s) 67
Eco57I CTGAAG 1 cut(s) 1280
Eco81I CCTNAGG 2 cut(s) 1143, 1745
Eco88I CYCGRG 2 cut(s) 59, 1322
Eco91I GGTNACC 1 cut(s) 655
EcoICRI GAGCTC 1 cut(s) 67
EcoNI CCTNNNNNAGG 1 cut(s) 1748
EcoO109I RGGNCCY 1 cut(s) 477
EcoO65I GGTNACC 1 cut(s) 655
EcoRII CCWGG 1 cut(s) 1120
EcoRV GATATC 2 cut(s) 796, 1850
EcoT14I CCWWGG 5 cut(s) 129, 750, 1605, 2101, 2154
EcoT22I ATGCAT 1 cut(s) 1255
EcoT38I GRGCYC 2 cut(s) 69, 1329
ErhI CCWWGG 5 cut(s) 129, 750, 1605, 2101, 2154
FaqI GGGAC 4 cut(s) 490, 1525, 1726, 1992
FauNDI CATATG 2 cut(s) 1909, 1974
FblI GTMKAC 1 cut(s) 1301
Fnu4HI GCNGC 9 cut(s) 105, 370, 454, 558, 561, 564, 1091, 1396, 2064
FokI GGATG 7 cut(s) 112, 164, 299, 589, 934, 1127, 1138
FriOI GRGCYC 2 cut(s) 69, 1329
Fsp4HI GCNGC 9 cut(s) 105, 370, 454, 558, 561, 564, 1091, 1396, 2064
FspBI CTAG 2 cut(s) 1035, 2118
GlaI GCGC 3 cut(s) 303, 399, 1580
GluI GCNGC 9 cut(s) 105, 370, 454, 558, 561, 564, 1091, 1396, 2064
GsaI CCCAGC 1 cut(s) 1506
GsuI CTGGAG 3 cut(s) 326, 713, 2045
HaeII RGCGCY 3 cut(s) 305, 401, 1582
HaeIII GGCC 3 cut(s) 456, 749, 1130
HapII CCGG 9 cut(s) 5, 25, 166, 251, 263, 395, 457, 1691, 1701
HgaI GACGC 1 cut(s) 349
HhaI GCGC 3 cut(s) 304, 400, 1581
Hin1I GRCGYC 1 cut(s) 360
Hin6I GCGC 3 cut(s) 302, 398, 1579
HinP1I GCGC 3 cut(s) 302, 398, 1579
HincII GTYRAC 4 cut(s) 630, 642, 1261, 1552
HindII GTYRAC 4 cut(s) 630, 642, 1261, 1552
HindIII AAGCTT 1 cut(s) 428
HpaII CCGG 9 cut(s) 5, 25, 166, 251, 263, 395, 457, 1691, 1701
HphI GGTGA 5 cut(s) 133, 592, 626, 632, 1690
Hpy99I CGWCG 3 cut(s) 338, 365, 505
HpyAV CCTTC 2 cut(s) 861, 1678
HpyCH4III ACNGT 7 cut(s) 145, 445, 634, 871, 1012, 1795, 2235
HpyCH4IV ACGT 2 cut(s) 471, 513
HpyF10VI GCNNNNNNNGC 9 cut(s) 64, 110, 366, 375, 406, 563, 1115, 2012, 2063
HpyF3I CTNAG 8 cut(s) 85, 401, 896, 1143, 1239, 1400, 1463, 1745
HpySE526I ACGT 2 cut(s) 471, 513
Hsp92I GRCGYC 1 cut(s) 360
HspAI GCGC 3 cut(s) 302, 398, 1579
Kpn2I TCCGGA 1 cut(s) 262
KpnI GGTACC 1 cut(s) 1691
KroI GCCGGC 1 cut(s) 4
KroNI GCCGGC 1 cut(s) 6
Kzo9I GATC 9 cut(s) 92, 184, 404, 414, 1138, 1198, 1468, 1717, 2073
LmnI GCTCC 5 cut(s) 64, 72, 350, 905, 929
Lsp1109I GCAGC 5 cut(s) 381, 544, 550, 1407, 2050
LweI GCATC 7 cut(s) 235, 853, 1105, 1405, 1621, 2050, 2189
MaeI CTAG 2 cut(s) 1035, 2118
MaeII ACGT 2 cut(s) 471, 513
MaeIII GTNAC 5 cut(s) 139, 316, 655, 845, 1403
MalI GATC 9 cut(s) 94, 186, 406, 416, 1140, 1200, 1470, 1719, 2075
MbiI CCGCTC 1 cut(s) 453
MboI GATC 9 cut(s) 92, 184, 404, 414, 1138, 1198, 1468, 1717, 2073
MboII GAAGA 9 cut(s) 111, 600, 1208, 1286, 1297, 1608, 1693, 1732, 1844
MfeI CAATTG 1 cut(s) 1761
MhlI GDGCHC 3 cut(s) 69, 1329, 2097
MluCI AATT 8 cut(s) 18, 952, 1491, 1570, 1761, 1783, 1813, 2135
MlyI GAGTC 6 cut(s) 77, 706, 1256, 1297, 1766, 1974
MmeI TCCRAC 7 cut(s) 120, 328, 343, 808, 883, 1777, 1814
Mph1103I ATGCAT 1 cut(s) 1255
MroI TCCGGA 1 cut(s) 262
MroNI GCCGGC 1 cut(s) 4
MroXI GAANNNNTTC 1 cut(s) 1600
MseI TTAA 4 cut(s) 525, 1173, 1185, 2145
MslI CAYNNNNRTG 2 cut(s) 146, 1230
MspA1I CMGCKG 3 cut(s) 372, 1991, 2015
MspCI CTTAAG 2 cut(s) 1172, 2144
MspI CCGG 9 cut(s) 5, 25, 166, 251, 263, 395, 457, 1691, 1701
MspR9I CCNGG 4 cut(s) 26, 251, 395, 1122
MunI CAATTG 1 cut(s) 1761
Mva1269I GAATGC 2 cut(s) 107, 679
MvaI CCWGG 1 cut(s) 1122
MwoI GCNNNNNNNGC 9 cut(s) 64, 110, 366, 375, 406, 563, 1115, 2012, 2063
NaeI GCCGGC 1 cut(s) 6
NciI CCSGG 3 cut(s) 26, 251, 395
NcoI CCATGG 2 cut(s) 750, 2101
NdeI CATATG 2 cut(s) 1909, 1974
NdeII GATC 9 cut(s) 92, 184, 404, 414, 1138, 1198, 1468, 1717, 2073
NgoMIV GCCGGC 1 cut(s) 4
NheI GCTAGC 1 cut(s) 1034
NlaIV GGNNCC 6 cut(s) 43, 170, 478, 885, 1603, 1689
NmuCI GTSAC 3 cut(s) 139, 845, 1403
NsiI ATGCAT 1 cut(s) 1255
OliI CACNNNNGTG 1 cut(s) 146
PaeR7I CTCGAG 1 cut(s) 59
PagI TCATGA 2 cut(s) 973, 1342
PaqCI CACCTGC 1 cut(s) 1741
PcsI WCGNNNNNNNCGW 1 cut(s) 45
PctI GAATGC 2 cut(s) 107, 679
PdiI GCCGGC 1 cut(s) 6
PdmI GAANNNNTTC 1 cut(s) 1600
PfeI GAWTC 5 cut(s) 72, 179, 755, 970, 1767
PflFI GACNNNGTC 1 cut(s) 86
PflMI CCANNNNNTGG 1 cut(s) 1796
PinAI ACCGGT 1 cut(s) 1690
PkrI GCNGC 9 cut(s) 106, 371, 455, 559, 562, 565, 1092, 1397, 2065
Ple19I CGATCG 1 cut(s) 417
PleI GAGTC 6 cut(s) 77, 706, 1256, 1296, 1765, 1974
PpsI GAGTC 6 cut(s) 77, 706, 1256, 1296, 1765, 1974
Ppu21I YACGTR 1 cut(s) 472
PpuMI RGGWCCY 1 cut(s) 477
PshBI ATTAAT 1 cut(s) 525
PsiI TTATAA 1 cut(s) 779
Psp124BI GAGCTC 1 cut(s) 69
Psp5II RGGWCCY 1 cut(s) 477
Psp6I CCWGG 1 cut(s) 1120
PspEI GGTNACC 1 cut(s) 655
PspFI CCCAGC 1 cut(s) 1502
PspGI CCWGG 1 cut(s) 1120
PspN4I GGNNCC 6 cut(s) 43, 170, 478, 885, 1603, 1689
PspPI GGNCC 5 cut(s) 253, 463, 477, 747, 2108
PspPPI RGGWCCY 1 cut(s) 477
PspXI VCTCGAGB 1 cut(s) 59
PsrI GAACNNNNNNTAC 2 cut(s) 34, 66
PstNI CAGNNNCTG 1 cut(s) 2063
PsyI GACNNNGTC 1 cut(s) 86
PvuI CGATCG 1 cut(s) 417
PvuII CAGCTG 2 cut(s) 1991, 2015
RsaI GTAC 8 cut(s) 497, 1217, 1568, 1647, 1689, 2046, 2142, 2237
RsaNI GTAC 8 cut(s) 496, 1216, 1567, 1646, 1688, 2045, 2141, 2236
RseI CAYNNNNRTG 2 cut(s) 146, 1230
SacI GAGCTC 1 cut(s) 69
SaqAI TTAA 4 cut(s) 525, 1173, 1185, 2145
SatI GCNGC 9 cut(s) 105, 370, 454, 558, 561, 564, 1091, 1396, 2064
Sau3AI GATC 9 cut(s) 92, 184, 404, 414, 1138, 1198, 1468, 1717, 2073
Sau96I GGNCC 5 cut(s) 253, 463, 477, 747, 2108
ScaI AGTACT 1 cut(s) 1647
SchI GAGTC 6 cut(s) 77, 706, 1256, 1297, 1766, 1974
ScrFI CCNGG 4 cut(s) 26, 251, 395, 1122
SduI GDGCHC 3 cut(s) 69, 1329, 2097
SfaNI GCATC 7 cut(s) 235, 853, 1105, 1405, 1621, 2050, 2189
SfcI CTRYAG 5 cut(s) 870, 1454, 1641, 1986, 1998
Sfr274I CTCGAG 1 cut(s) 59
SinI GGWCC 4 cut(s) 253, 463, 477, 2108
SlaI CTCGAG 1 cut(s) 59
SmiMI CAYNNNNRTG 2 cut(s) 146, 1230
SmlI CTYRAG 5 cut(s) 59, 593, 1172, 1372, 2144
SmoI CTYRAG 5 cut(s) 59, 593, 1172, 1372, 2144
SnaBI TACGTA 1 cut(s) 472
Sse9I AATT 8 cut(s) 18, 952, 1491, 1570, 1761, 1783, 1813, 2135
SspI AATATT 1 cut(s) 1029
SspMI CTAG 2 cut(s) 1035, 2118
SstI GAGCTC 1 cut(s) 69
StyD4I CCNGG 4 cut(s) 24, 249, 393, 1120
StyI CCWWGG 5 cut(s) 129, 750, 1605, 2101, 2154
TaaI ACNGT 7 cut(s) 145, 445, 634, 871, 1012, 1795, 2235
TaiI ACGT 2 cut(s) 474, 516
TaqI TCGA 5 cut(s) 60, 215, 503, 1852, 2183
TasI AATT 8 cut(s) 18, 952, 1491, 1570, 1761, 1783, 1813, 2135
TatI WGTACW 5 cut(s) 1566, 1645, 2044, 2140, 2235
TauI GCSGC 4 cut(s) 107, 456, 563, 1093
TfiI GAWTC 5 cut(s) 72, 179, 755, 970, 1767
Tru1I TTAA 4 cut(s) 525, 1173, 1185, 2145
Tru9I TTAA 4 cut(s) 525, 1173, 1185, 2145
TscAI CASTG 2 cut(s) 1002, 1680
TseFI GTSAC 3 cut(s) 139, 845, 1403
TseI GCWGC 5 cut(s) 369, 557, 563, 1395, 2063
Tsp45I GTSAC 3 cut(s) 139, 845, 1403
TspGWI ACGGA 2 cut(s) 148, 476
TspRI CASTG 2 cut(s) 1002, 1680
Tth111I GACNNNGTC 1 cut(s) 86
Van91I CCANNNNNTGG 1 cut(s) 1796
Vha464I CTTAAG 2 cut(s) 1172, 2144
VpaK11BI GGWCC 4 cut(s) 253, 463, 477, 2108
VspI ATTAAT 1 cut(s) 525
XagI CCTNNNNNAGG 1 cut(s) 1748
XapI RAATTY 3 cut(s) 18, 1783, 2135
XcmI CCANNNNNNNNNTGG 1 cut(s) 757
XhoI CTCGAG 1 cut(s) 59
XmiI GTMKAC 1 cut(s) 1301
XmnI GAANNNNTTC 1 cut(s) 1600
XspI CTAG 2 cut(s) 1035, 2118
ZrmI AGTACT 1 cut(s) 1647
Zsp2I ATGCAT 1 cut(s) 1255
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.