Rorug02G0093300

Uncharacterised protein family (UPF0261)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0001393
Physical Location & Seq
Forward (+)
7659187 .. 7660577
1391 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0093300.1

Sequence Viewer

Length: 510 bp
ATGGCAGCGAAAACCAACATGTTGCCTATAATCAGCAAGATGTATTGTTCTTCTGCACAGGGTGTGTTTGTAGTTAGGAGGAGGCCACATGCGATAAATGGAGGTGGTTTTGTAGTCACAGATTGTAGTCAGAAGGTTGCTTTCAGGGTTGATGGATGTGGAATTCTGGGAAAAAAGGATGAGCTGATTCTGAGAGATGGAGATGGAGACGCCTTGCTTCTCATTCGTCGAAAGGGAGCGATGGTTGAGGCGCTAAGCATTTACAGGAAATGGAAGGGCTATGCTTTAGACTATGAAGGATCACAGGAGTTGGTTTTCAGTTTGAAAGAGCCAAACTCATGCCTGGCAAGGACTCAAGCAATCAGAATCTCGACAAAGAGAACACAAAACAAAGACTGGGACTTCGAGATAAAGGGCCATTTCCCGGATAAGGCTTGTAGCATTGTTGACTGTAAAGGCAACGTAATGGCACAGTTGGATTTCTTACTTCTTACAATGGAGCACAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PEP_hydrolase UPF0261

Protein Analysis

169

Amino Acids

18.92

Weight (kDa)

9.18

Isoelectric Point (pI)

34.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LOR PF04525 23 - 158 2.9e-20 LURP-one-related
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 307
AcsI RAATTY 1 cut(s) 162
AcyI GRCGYC 1 cut(s) 210
AdeI CACNNNGTG 1 cut(s) 62
AfiI CCNNNNNNNGG 2 cut(s) 424, 430
AflIII ACRYGT 1 cut(s) 18
AgsI TTSAA 1 cut(s) 325
AjnI CCWGG 1 cut(s) 342
AluBI AGCT 1 cut(s) 184
AluI AGCT 1 cut(s) 184
Alw21I GWGCWC 1 cut(s) 504
Alw26I GTCTC 1 cut(s) 201
AlwI GGATC 1 cut(s) 307
AoxI GGCC 2 cut(s) 83, 415
ApeKI GCWGC 1 cut(s) 5
ApoI RAATTY 1 cut(s) 162
ArsI GACNNNNNNTTYG 2 cut(s) 386, 418
AspLEI GCGC 1 cut(s) 253
AspS9I GGNCC 1 cut(s) 415
AsuC2I CCSGG 1 cut(s) 425
Bbv12I GWGCWC 1 cut(s) 504
BbvI GCAGC 1 cut(s) 17
BccI CCATC 4 cut(s) 146, 191, 197, 235
BciT130I CCWGG 1 cut(s) 344
BcnI CCSGG 1 cut(s) 425
BcoDI GTCTC 1 cut(s) 201
BfoI RGCGCY 1 cut(s) 254
BisI GCNGC 1 cut(s) 6
BlpI GCTNAGC 1 cut(s) 254
BlsI GCNGC 1 cut(s) 7
Bme1390I CCNGG 2 cut(s) 344, 425
BmgT120I GGNCC 1 cut(s) 415
BmrFI CCNGG 2 cut(s) 344, 425
BmrI ACTGGG 1 cut(s) 406
BmuI ACTGGG 1 cut(s) 406
BplI GAGNNNNNCTC 2 cut(s) 320, 352
Bpu1102I GCTNAGC 1 cut(s) 254
BpuEI CTTGAG 1 cut(s) 339
BpuMI CCSGG 1 cut(s) 425
BsaHI GRCGYC 1 cut(s) 210
BsaXI ACNNNNNCTCC 2 cut(s) 228, 258
Bsc4I CCNNNNNNNGG 2 cut(s) 424, 430
Bse1I ACTGG 1 cut(s) 401
BseBI CCWGG 1 cut(s) 344
BseGI GGATG 2 cut(s) 161, 184
BseLI CCNNNNNNNGG 2 cut(s) 424, 430
BseMII CTCAG 1 cut(s) 182
BseNI ACTGG 1 cut(s) 401
BseRI GAGGAG 1 cut(s) 94
BseXI GCAGC 1 cut(s) 17
BsgI GTGCAG 1 cut(s) 39
BshFI GGCC 2 cut(s) 85, 417
BsiHKAI GWGCWC 1 cut(s) 504
BsiSI CCGG 1 cut(s) 425
BslFI GGGAC 1 cut(s) 413
BslI CCNNNNNNNGG 2 cut(s) 424, 430
BsmAI GTCTC 1 cut(s) 201
BsmBI CGTCTC 1 cut(s) 201
BsmFI GGGAC 1 cut(s) 413
BsnI GGCC 2 cut(s) 85, 417
Bsp1286I GDGCHC 1 cut(s) 504
Bsp143I GATC 1 cut(s) 299
Bsp1720I GCTNAGC 1 cut(s) 254
BspANI GGCC 2 cut(s) 85, 417
BspCNI CTCAG 1 cut(s) 183
BspPI GGATC 1 cut(s) 307
BsrI ACTGG 1 cut(s) 401
BssMI GATC 1 cut(s) 299
BssNI GRCGYC 1 cut(s) 210
Bst2UI CCWGG 1 cut(s) 344
Bst4CI ACNGT 2 cut(s) 452, 474
BstACI GRCGYC 1 cut(s) 210
BstDEI CTNAG 2 cut(s) 191, 254
BstF5I GGATG 2 cut(s) 161, 184
BstH2I RGCGCY 1 cut(s) 254
BstHHI GCGC 1 cut(s) 253
BstKTI GATC 1 cut(s) 302
BstMAI GTCTC 1 cut(s) 201
BstMBI GATC 1 cut(s) 299
BstNI CCWGG 1 cut(s) 344
BstNSI RCATGY 2 cut(s) 22, 92
BstSCI CCNGG 2 cut(s) 342, 423
BstV1I GCAGC 1 cut(s) 17
BsuRI GGCC 2 cut(s) 85, 417
BtgZI GCGATG 1 cut(s) 254
BtsCI GGATG 2 cut(s) 161, 184
CfoI GCGC 1 cut(s) 253
Cfr13I GGNCC 1 cut(s) 415
CseI GACGC 1 cut(s) 218
CviAII CATG 3 cut(s) 19, 89, 339
CviJI RGCY 6 cut(s) 85, 184, 279, 331, 417, 434
CviKI_1 RGCY 6 cut(s) 85, 184, 279, 331, 417, 434
DdeI CTNAG 2 cut(s) 191, 254
DpnI GATC 1 cut(s) 301
DpnII GATC 1 cut(s) 299
DraIII CACNNNGTG 1 cut(s) 62
EcoRI GAATTC 1 cut(s) 162
EcoRII CCWGG 1 cut(s) 342
Esp3I CGTCTC 1 cut(s) 201
FaeI CATG 3 cut(s) 22, 92, 342
FaiI YATR 6 cut(s) 20, 29, 90, 282, 294, 340
FaqI GGGAC 1 cut(s) 413
FatI CATG 3 cut(s) 18, 88, 338
Fnu4HI GCNGC 1 cut(s) 6
FokI GGATG 2 cut(s) 168, 191
Fsp4HI GCNGC 1 cut(s) 6
GlaI GCGC 1 cut(s) 252
GluI GCNGC 1 cut(s) 6
HaeII RGCGCY 1 cut(s) 254
HaeIII GGCC 2 cut(s) 85, 417
HapII CCGG 1 cut(s) 425
HgaI GACGC 1 cut(s) 218
HhaI GCGC 1 cut(s) 253
Hin1I GRCGYC 1 cut(s) 210
Hin1II CATG 3 cut(s) 22, 92, 342
Hin6I GCGC 1 cut(s) 251
HinP1I GCGC 1 cut(s) 251
HincII GTYRAC 1 cut(s) 448
HindII GTYRAC 1 cut(s) 448
HinfI GANTC 3 cut(s) 187, 352, 366
HpaII CCGG 1 cut(s) 425
Hpy166II GTNNAC 1 cut(s) 448
Hpy188I TCNGA 3 cut(s) 132, 192, 365
Hpy188III TCNNGA 2 cut(s) 370, 406
Hpy8I GTNNAC 1 cut(s) 448
Hpy99I CGWCG 1 cut(s) 231
HpyAV CCTTC 3 cut(s) 127, 268, 290
HpyCH4III ACNGT 2 cut(s) 452, 474
HpyCH4IV ACGT 1 cut(s) 462
HpyCH4V TGCA 1 cut(s) 56
HpyF3I CTNAG 2 cut(s) 191, 254
HpySE526I ACGT 1 cut(s) 462
Hsp92I GRCGYC 1 cut(s) 210
Hsp92II CATG 3 cut(s) 22, 92, 342
HspAI GCGC 1 cut(s) 251
Kzo9I GATC 1 cut(s) 299
LmnI GCTCC 2 cut(s) 236, 499
LpnPI CCDG 9 cut(s) 44, 130, 152, 250, 290, 329, 356, 382, 438
Lsp1109I GCAGC 1 cut(s) 17
MaeII ACGT 1 cut(s) 462
MaeIII GTNAC 1 cut(s) 115
MalI GATC 1 cut(s) 301
MboI GATC 1 cut(s) 299
MboII GAAGA 1 cut(s) 42
MhlI GDGCHC 1 cut(s) 504
MluCI AATT 1 cut(s) 162
MlyI GAGTC 1 cut(s) 346
MmeI TCCRAC 1 cut(s) 456
MnlI CCTC 4 cut(s) 72, 75, 95, 241
MspI CCGG 1 cut(s) 425
MspR9I CCNGG 2 cut(s) 344, 425
MvaI CCWGG 1 cut(s) 344
NciI CCSGG 1 cut(s) 425
NdeII GATC 1 cut(s) 299
NlaIII CATG 3 cut(s) 22, 92, 342
NmuCI GTSAC 1 cut(s) 115
NspI RCATGY 2 cut(s) 22, 92
PciI ACATGT 1 cut(s) 18
PfeI GAWTC 2 cut(s) 187, 366
PfoI TCCNGGA 1 cut(s) 423
PkrI GCNGC 1 cut(s) 7
PleI GAGTC 1 cut(s) 346
PpsI GAGTC 1 cut(s) 346
PscI ACATGT 1 cut(s) 18
Psp6I CCWGG 1 cut(s) 342
PspGI CCWGG 1 cut(s) 342
PspPI GGNCC 1 cut(s) 415
SatI GCNGC 1 cut(s) 6
Sau3AI GATC 1 cut(s) 299
Sau96I GGNCC 1 cut(s) 415
SchI GAGTC 1 cut(s) 346
ScrFI CCNGG 2 cut(s) 344, 425
SduI GDGCHC 1 cut(s) 504
SetI ASST 4 cut(s) 106, 138, 186, 465
SmlI CTYRAG 1 cut(s) 354
SmoI CTYRAG 1 cut(s) 354
Sse9I AATT 1 cut(s) 162
StyD4I CCNGG 2 cut(s) 342, 423
TaaI ACNGT 2 cut(s) 452, 474
TaiI ACGT 1 cut(s) 465
TaqI TCGA 3 cut(s) 229, 371, 405
TasI AATT 1 cut(s) 162
TfiI GAWTC 2 cut(s) 187, 366
TseFI GTSAC 1 cut(s) 115
TseI GCWGC 1 cut(s) 5
Tsp45I GTSAC 1 cut(s) 115
TspDTI ATGAA 1 cut(s) 309
XapI RAATTY 1 cut(s) 162
XceI RCATGY 2 cut(s) 22, 92
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.