Phosphorylates Ins(1,3,4,5,6)P5 at position 2 to form Ins(1,2,3,4,5,6)P6 (InsP6 or phytate)
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 324 |
| AclWI | GGATC | 1 cut(s) | 131 |
| AcoI | YGGCCR | 1 cut(s) | 247 |
| AcuI | CTGAAG | 1 cut(s) | 333 |
| AflIII | ACRYGT | 1 cut(s) | 278 |
| AgsI | TTSAA | 1 cut(s) | 33 |
| AjnI | CCWGG | 1 cut(s) | 93 |
| AluBI | AGCT | 1 cut(s) | 22 |
| AluI | AGCT | 1 cut(s) | 22 |
| AlwI | GGATC | 1 cut(s) | 131 |
| AoxI | GGCC | 2 cut(s) | 247, 253 |
| ApeKI | GCWGC | 1 cut(s) | 22 |
| AspS9I | GGNCC | 2 cut(s) | 97, 200 |
| AsuC2I | CCSGG | 1 cut(s) | 127 |
| AsuHPI | GGTGA | 1 cut(s) | 61 |
| AvaII | GGWCC | 2 cut(s) | 97, 200 |
| BbvI | GCAGC | 1 cut(s) | 9 |
| BccI | CCATC | 1 cut(s) | 311 |
| BceAI | ACGGC | 1 cut(s) | 262 |
| BciT130I | CCWGG | 1 cut(s) | 95 |
| BcnI | CCSGG | 1 cut(s) | 127 |
| BfmI | CTRYAG | 1 cut(s) | 6 |
| BisI | GCNGC | 1 cut(s) | 23 |
| BlsI | GCNGC | 1 cut(s) | 24 |
| Bme1390I | CCNGG | 2 cut(s) | 95, 127 |
| Bme18I | GGWCC | 2 cut(s) | 97, 200 |
| BmgT120I | GGNCC | 2 cut(s) | 97, 200 |
| BmiI | GGNNCC | 1 cut(s) | 99 |
| BmrFI | CCNGG | 2 cut(s) | 95, 127 |
| BmsI | GCATC | 1 cut(s) | 204 |
| BpuMI | CCSGG | 1 cut(s) | 127 |
| BsaJI | CCNNGG | 3 cut(s) | 126, 250, 272 |
| BsaWI | WCCGGW | 1 cut(s) | 139 |
| BseBI | CCWGG | 1 cut(s) | 95 |
| BseDI | CCNNGG | 3 cut(s) | 126, 250, 272 |
| BseGI | GGATG | 1 cut(s) | 232 |
| BseXI | GCAGC | 1 cut(s) | 9 |
| BsgI | GTGCAG | 1 cut(s) | 58 |
| BshFI | GGCC | 2 cut(s) | 249, 255 |
| BsiSI | CCGG | 3 cut(s) | 126, 140, 212 |
| BsnI | GGCC | 2 cut(s) | 249, 255 |
| Bsp143I | GATC | 1 cut(s) | 136 |
| Bsp19I | CCATGG | 1 cut(s) | 250 |
| BspANI | GGCC | 2 cut(s) | 249, 255 |
| BspLI | GGNNCC | 1 cut(s) | 99 |
| BspPI | GGATC | 1 cut(s) | 131 |
| BssECI | CCNNGG | 3 cut(s) | 126, 250, 272 |
| BssMI | GATC | 1 cut(s) | 136 |
| BssT1I | CCWWGG | 2 cut(s) | 250, 272 |
| Bst2UI | CCWGG | 1 cut(s) | 95 |
| Bst4CI | ACNGT | 5 cut(s) | 7, 70, 115, 121, 335 |
| Bst6I | CTCTTC | 1 cut(s) | 210 |
| BstDSI | CCRYGG | 1 cut(s) | 250 |
| BstF5I | GGATG | 1 cut(s) | 232 |
| BstKTI | GATC | 1 cut(s) | 139 |
| BstMBI | GATC | 1 cut(s) | 136 |
| BstNI | CCWGG | 1 cut(s) | 95 |
| BstNSI | RCATGY | 1 cut(s) | 282 |
| BstSCI | CCNGG | 2 cut(s) | 93, 125 |
| BstSFI | CTRYAG | 1 cut(s) | 6 |
| BstV1I | GCAGC | 1 cut(s) | 9 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 94 |
| BsuRI | GGCC | 2 cut(s) | 249, 255 |
| BtgI | CCRYGG | 1 cut(s) | 250 |
| BtsCI | GGATG | 1 cut(s) | 232 |
| BtsIMutI | CAGTG | 1 cut(s) | 120 |
| Cfr13I | GGNCC | 2 cut(s) | 97, 200 |
| CviAII | CATG | 2 cut(s) | 251, 279 |
| CviJI | RGCY | 5 cut(s) | 22, 134, 211, 249, 255 |
| CviKI_1 | RGCY | 5 cut(s) | 22, 134, 211, 249, 255 |
| DpnI | GATC | 1 cut(s) | 138 |
| DpnII | GATC | 1 cut(s) | 136 |
| EaeI | YGGCCR | 1 cut(s) | 247 |
| Eam1104I | CTCTTC | 1 cut(s) | 210 |
| EarI | CTCTTC | 1 cut(s) | 210 |
| Eco130I | CCWWGG | 2 cut(s) | 250, 272 |
| Eco47I | GGWCC | 2 cut(s) | 97, 200 |
| Eco57I | CTGAAG | 1 cut(s) | 333 |
| EcoRII | CCWGG | 1 cut(s) | 93 |
| EcoT14I | CCWWGG | 2 cut(s) | 250, 272 |
| ErhI | CCWWGG | 2 cut(s) | 250, 272 |
| FaeI | CATG | 2 cut(s) | 254, 282 |
| FaiI | YATR | 5 cut(s) | 252, 280, 303, 324, 344 |
| FatI | CATG | 2 cut(s) | 250, 278 |
| Fnu4HI | GCNGC | 1 cut(s) | 23 |
| FokI | GGATG | 1 cut(s) | 239 |
| Fsp4HI | GCNGC | 1 cut(s) | 23 |
| GluI | GCNGC | 1 cut(s) | 23 |
| HaeIII | GGCC | 2 cut(s) | 249, 255 |
| HapII | CCGG | 3 cut(s) | 126, 140, 212 |
| Hin1II | CATG | 2 cut(s) | 254, 282 |
| HpaII | CCGG | 3 cut(s) | 126, 140, 212 |
| HphI | GGTGA | 1 cut(s) | 61 |
| Hpy188I | TCNGA | 1 cut(s) | 173 |
| HpyAV | CCTTC | 2 cut(s) | 68, 217 |
| HpyCH4III | ACNGT | 5 cut(s) | 7, 70, 115, 121, 335 |
| HpyCH4V | TGCA | 1 cut(s) | 39 |
| Hsp92II | CATG | 2 cut(s) | 254, 282 |
| Kzo9I | GATC | 1 cut(s) | 136 |
| LpnPI | CCDG | 7 cut(s) | 25, 80, 107, 139, 153, 225, 283 |
| Lsp1109I | GCAGC | 1 cut(s) | 9 |
| LweI | GCATC | 1 cut(s) | 204 |
| MaeIII | GTNAC | 2 cut(s) | 115, 121 |
| MalI | GATC | 1 cut(s) | 138 |
| MboI | GATC | 1 cut(s) | 136 |
| MboII | GAAGA | 4 cut(s) | 227, 232, 244, 326 |
| MluCI | AATT | 1 cut(s) | 328 |
| MnlI | CCTC | 1 cut(s) | 213 |
| MspI | CCGG | 3 cut(s) | 126, 140, 212 |
| MspR9I | CCNGG | 2 cut(s) | 95, 127 |
| MvaI | CCWGG | 1 cut(s) | 95 |
| NciI | CCSGG | 1 cut(s) | 127 |
| NcoI | CCATGG | 1 cut(s) | 250 |
| NdeII | GATC | 1 cut(s) | 136 |
| NlaIII | CATG | 2 cut(s) | 254, 282 |
| NlaIV | GGNNCC | 1 cut(s) | 99 |
| NmuCI | GTSAC | 1 cut(s) | 115 |
| NspI | RCATGY | 1 cut(s) | 282 |
| PciI | ACATGT | 1 cut(s) | 278 |
| PfoI | TCCNGGA | 1 cut(s) | 93 |
| PkrI | GCNGC | 1 cut(s) | 24 |
| PscI | ACATGT | 1 cut(s) | 278 |
| PsiI | TTATAA | 1 cut(s) | 324 |
| Psp6I | CCWGG | 1 cut(s) | 93 |
| PspGI | CCWGG | 1 cut(s) | 93 |
| PspN4I | GGNNCC | 1 cut(s) | 99 |
| PspPI | GGNCC | 2 cut(s) | 97, 200 |
| SatI | GCNGC | 1 cut(s) | 23 |
| Sau3AI | GATC | 1 cut(s) | 136 |
| Sau96I | GGNCC | 2 cut(s) | 97, 200 |
| ScrFI | CCNGG | 2 cut(s) | 95, 127 |
| SetI | ASST | 3 cut(s) | 24, 51, 150 |
| SfaNI | GCATC | 1 cut(s) | 204 |
| SfcI | CTRYAG | 1 cut(s) | 6 |
| SinI | GGWCC | 2 cut(s) | 97, 200 |
| Sse9I | AATT | 1 cut(s) | 328 |
| StyD4I | CCNGG | 2 cut(s) | 93, 125 |
| StyI | CCWWGG | 2 cut(s) | 250, 272 |
| TaaI | ACNGT | 5 cut(s) | 7, 70, 115, 121, 335 |
| TaqII | GACCGA | 1 cut(s) | 188 |
| TasI | AATT | 1 cut(s) | 328 |
| TscAI | CASTG | 1 cut(s) | 120 |
| TseFI | GTSAC | 1 cut(s) | 115 |
| TseI | GCWGC | 1 cut(s) | 22 |
| Tsp45I | GTSAC | 1 cut(s) | 115 |
| TspDTI | ATGAA | 1 cut(s) | 68 |
| TspGWI | ACGGA | 1 cut(s) | 328 |
| TspRI | CASTG | 1 cut(s) | 120 |
| VpaK11BI | GGWCC | 2 cut(s) | 97, 200 |
| XceI | RCATGY | 1 cut(s) | 282 |