Phosphorylates Ins(1,3,4,5,6)P5 at position 2 to form Ins(1,2,3,4,5,6)P6 (InsP6 or phytate)
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 2 cut(s) | 194, 215 |
| AclWI | GGATC | 1 cut(s) | 140 |
| AfaI | GTAC | 1 cut(s) | 329 |
| AlwI | GGATC | 1 cut(s) | 140 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 357 |
| ApeKI | GCWGC | 1 cut(s) | 191 |
| AspS9I | GGNCC | 1 cut(s) | 209 |
| AsuHPI | GGTGA | 3 cut(s) | 70, 136, 181 |
| AvaII | GGWCC | 1 cut(s) | 209 |
| BbvI | GCAGC | 1 cut(s) | 178 |
| BccI | CCATC | 1 cut(s) | 239 |
| BisI | GCNGC | 1 cut(s) | 192 |
| BlsI | GCNGC | 1 cut(s) | 193 |
| Bme18I | GGWCC | 1 cut(s) | 209 |
| BmgT120I | GGNCC | 1 cut(s) | 209 |
| BmrI | ACTGGG | 1 cut(s) | 144 |
| BmsI | GCATC | 1 cut(s) | 130 |
| BmuI | ACTGGG | 1 cut(s) | 144 |
| BsaAI | YACGTR | 1 cut(s) | 331 |
| BsaBI | GATNNNNATC | 1 cut(s) | 144 |
| Bse1I | ACTGG | 2 cut(s) | 139, 149 |
| Bse8I | GATNNNNATC | 1 cut(s) | 144 |
| BseGI | GGATG | 3 cut(s) | 145, 238, 250 |
| BseJI | GATNNNNATC | 1 cut(s) | 144 |
| BseMII | CTCAG | 1 cut(s) | 213 |
| BseNI | ACTGG | 2 cut(s) | 139, 149 |
| BseXI | GCAGC | 1 cut(s) | 178 |
| BsgI | GTGCAG | 1 cut(s) | 67 |
| Bsp143I | GATC | 1 cut(s) | 145 |
| BspACI | CCGC | 2 cut(s) | 194, 215 |
| BspCNI | CTCAG | 1 cut(s) | 214 |
| BspPI | GGATC | 1 cut(s) | 140 |
| BsrI | ACTGG | 2 cut(s) | 139, 149 |
| BssMI | GATC | 1 cut(s) | 145 |
| Bst4CI | ACNGT | 6 cut(s) | 16, 79, 124, 130, 353, 359 |
| BstBAI | YACGTR | 1 cut(s) | 331 |
| BstC8I | GCNNGC | 1 cut(s) | 219 |
| BstDEI | CTNAG | 1 cut(s) | 222 |
| BstF5I | GGATG | 3 cut(s) | 145, 238, 250 |
| BstKTI | GATC | 1 cut(s) | 148 |
| BstMBI | GATC | 1 cut(s) | 145 |
| BstV1I | GCAGC | 1 cut(s) | 178 |
| BtsCI | GGATG | 3 cut(s) | 145, 238, 250 |
| BtsIMutI | CAGTG | 1 cut(s) | 30 |
| Cac8I | GCNNGC | 1 cut(s) | 219 |
| CaiI | CAGNNNCTG | 1 cut(s) | 357 |
| Cfr13I | GGNCC | 1 cut(s) | 209 |
| Csp6I | GTAC | 1 cut(s) | 328 |
| CviAII | CATG | 2 cut(s) | 306, 344 |
| CviJI | RGCY | 2 cut(s) | 221, 304 |
| CviKI_1 | RGCY | 2 cut(s) | 221, 304 |
| CviQI | GTAC | 1 cut(s) | 328 |
| DdeI | CTNAG | 1 cut(s) | 222 |
| DpnI | GATC | 1 cut(s) | 147 |
| DpnII | GATC | 1 cut(s) | 145 |
| Eco47I | GGWCC | 1 cut(s) | 209 |
| FaeI | CATG | 2 cut(s) | 309, 347 |
| FaiI | YATR | 4 cut(s) | 120, 292, 307, 345 |
| FatI | CATG | 2 cut(s) | 305, 343 |
| Fnu4HI | GCNGC | 1 cut(s) | 192 |
| FokI | GGATG | 3 cut(s) | 152, 245, 257 |
| Fsp4HI | GCNGC | 1 cut(s) | 192 |
| GluI | GCNGC | 1 cut(s) | 192 |
| Hin1II | CATG | 2 cut(s) | 309, 347 |
| HinfI | GANTC | 1 cut(s) | 334 |
| HphI | GGTGA | 3 cut(s) | 70, 136, 181 |
| Hpy188III | TCNNGA | 2 cut(s) | 163, 338 |
| HpyAV | CCTTC | 7 cut(s) | 77, 160, 223, 235, 247, 259, 271 |
| HpyCH4III | ACNGT | 6 cut(s) | 16, 79, 124, 130, 353, 359 |
| HpyCH4IV | ACGT | 2 cut(s) | 282, 330 |
| HpyCH4V | TGCA | 2 cut(s) | 48, 74 |
| HpyF3I | CTNAG | 1 cut(s) | 222 |
| HpySE526I | ACGT | 2 cut(s) | 282, 330 |
| Hsp92II | CATG | 2 cut(s) | 309, 347 |
| Kzo9I | GATC | 1 cut(s) | 145 |
| LpnPI | CCDG | 5 cut(s) | 34, 120, 162, 203, 351 |
| Lsp1109I | GCAGC | 1 cut(s) | 178 |
| LweI | GCATC | 1 cut(s) | 130 |
| MaeII | ACGT | 2 cut(s) | 282, 330 |
| MaeIII | GTNAC | 4 cut(s) | 88, 124, 130, 353 |
| MalI | GATC | 1 cut(s) | 147 |
| MboI | GATC | 1 cut(s) | 145 |
| MboII | GAAGA | 6 cut(s) | 19, 238, 250, 262, 274, 286 |
| MluCI | AATT | 1 cut(s) | 176 |
| MnlI | CCTC | 3 cut(s) | 113, 165, 222 |
| MseI | TTAA | 1 cut(s) | 179 |
| NdeII | GATC | 1 cut(s) | 145 |
| NlaIII | CATG | 2 cut(s) | 309, 347 |
| NmuCI | GTSAC | 1 cut(s) | 124 |
| PfeI | GAWTC | 1 cut(s) | 334 |
| PkrI | GCNGC | 1 cut(s) | 193 |
| Ppu21I | YACGTR | 1 cut(s) | 331 |
| PspPI | GGNCC | 1 cut(s) | 209 |
| PstNI | CAGNNNCTG | 1 cut(s) | 357 |
| RsaI | GTAC | 1 cut(s) | 329 |
| RsaNI | GTAC | 1 cut(s) | 328 |
| SaqAI | TTAA | 1 cut(s) | 179 |
| SatI | GCNGC | 1 cut(s) | 192 |
| Sau3AI | GATC | 1 cut(s) | 145 |
| Sau96I | GGNCC | 1 cut(s) | 209 |
| SetI | ASST | 4 cut(s) | 60, 171, 285, 333 |
| SfaNI | GCATC | 1 cut(s) | 130 |
| SinI | GGWCC | 1 cut(s) | 209 |
| Sse9I | AATT | 1 cut(s) | 176 |
| SsiI | CCGC | 2 cut(s) | 194, 215 |
| TaaI | ACNGT | 6 cut(s) | 16, 79, 124, 130, 353, 359 |
| TaiI | ACGT | 2 cut(s) | 285, 333 |
| TaqI | TCGA | 2 cut(s) | 5, 105 |
| TaqII | GACCGA | 1 cut(s) | 197 |
| TasI | AATT | 1 cut(s) | 176 |
| TfiI | GAWTC | 1 cut(s) | 334 |
| Tru1I | TTAA | 1 cut(s) | 179 |
| Tru9I | TTAA | 1 cut(s) | 179 |
| TscAI | CASTG | 1 cut(s) | 30 |
| TseFI | GTSAC | 1 cut(s) | 124 |
| TseI | GCWGC | 1 cut(s) | 191 |
| Tsp45I | GTSAC | 1 cut(s) | 124 |
| TspDTI | ATGAA | 1 cut(s) | 77 |
| TspGWI | ACGGA | 1 cut(s) | 352 |
| TspRI | CASTG | 1 cut(s) | 30 |
| VpaK11BI | GGWCC | 1 cut(s) | 209 |