MD02G1143000.v1.1

Heavy-metal-associated domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0001393
Physical Location & Seq
Reverse (-)
11885636 .. 11886393
758 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1143000.v1.1.491

Sequence Viewer

Length: 372 bp
ATGGTACAAAGGACTGTCCTCAAGGTTGACATACATTGCCAAAAATGCCAGAGGAAGCTTCTCAAGGCAGTGTCTGTCCTACAAGGTGTGGATAAACTTGAAGTTGATGCAGCCAAAGGAACTTTGACGGTAACAGGCGACTGTGATCCCTATGAAATAATTGTTCGAACTAGACAAGCGTGCAAATATGCCGAAGTAGTGAGTATTGGGCCTCCTCCTGCAGAAAAGAAGCCCGAACAGAAGAAGCGTGAAACAAAAGACCCCAAGGTCGAGCCCTATCACATGCCCTATTACCCTGAGCCATGTCAAAGGTGGGGCATTGTGAGGGTGGATTCCTGTTATGACCACAACCCTTACTGCTCAATCATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HMA

Protein Analysis

124

Amino Acids

14.08

Weight (kDa)

8.65

Isoelectric Point (pI)

38.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 7 - 63 1.6e-11 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 266
AclWI GGATC 1 cut(s) 140
AfaI GTAC 1 cut(s) 6
AgsI TTSAA 1 cut(s) 101
AluBI AGCT 1 cut(s) 58
AluI AGCT 1 cut(s) 58
AlwI GGATC 1 cut(s) 140
AlwNI CAGNNNCTG 1 cut(s) 74
AoxI GGCC 1 cut(s) 209
ApeKI GCWGC 1 cut(s) 110
AspS9I GGNCC 1 cut(s) 209
AsuII TTCGAA 1 cut(s) 166
BanII GRGCYC 1 cut(s) 276
BbvI GCAGC 1 cut(s) 122
BfaI CTAG 1 cut(s) 171
BfmI CTRYAG 1 cut(s) 219
BisI GCNGC 1 cut(s) 111
BlsI GCNGC 1 cut(s) 112
BmgT120I GGNCC 1 cut(s) 209
BmsI GCATC 1 cut(s) 97
Bpu10I CCTNAGC 1 cut(s) 297
Bpu14I TTCGAA 1 cut(s) 166
BpuEI CTTGAG 2 cut(s) 5, 47
BsaJI CCNNGG 1 cut(s) 264
Bse3DI GCAATG 1 cut(s) 34
BseDI CCNNGG 1 cut(s) 264
BseMI GCAATG 1 cut(s) 34
BseMII CTCAG 1 cut(s) 288
BseRI GAGGAG 1 cut(s) 204
BseXI GCAGC 1 cut(s) 122
BshFI GGCC 1 cut(s) 211
BsnI GGCC 1 cut(s) 211
Bsp119I TTCGAA 1 cut(s) 166
Bsp1286I GDGCHC 1 cut(s) 276
Bsp143I GATC 1 cut(s) 145
BspANI GGCC 1 cut(s) 211
BspCNI CTCAG 1 cut(s) 289
BspMAI CTGCAG 1 cut(s) 223
BspPI GGATC 1 cut(s) 140
BspT104I TTCGAA 1 cut(s) 166
BsrDI GCAATG 1 cut(s) 34
BssECI CCNNGG 1 cut(s) 264
BssMI GATC 1 cut(s) 145
BssT1I CCWWGG 1 cut(s) 264
Bst4CI ACNGT 3 cut(s) 16, 130, 143
BstBI TTCGAA 1 cut(s) 166
BstC8I GCNNGC 1 cut(s) 181
BstDEI CTNAG 1 cut(s) 297
BstKTI GATC 1 cut(s) 148
BstMBI GATC 1 cut(s) 145
BstMWI GCNNNNNNNGC 1 cut(s) 45
BstNSI RCATGY 1 cut(s) 286
BstSFI CTRYAG 1 cut(s) 219
BstV1I GCAGC 1 cut(s) 122
BsuRI GGCC 1 cut(s) 211
BtsI GCAGTG 1 cut(s) 75
BtsIMutI CAGTG 1 cut(s) 75
Cac8I GCNNGC 1 cut(s) 181
CaiI CAGNNNCTG 1 cut(s) 74
Cfr13I GGNCC 1 cut(s) 209
Csp6I GTAC 1 cut(s) 5
CviAII CATG 3 cut(s) 283, 303, 367
CviJI RGCY 6 cut(s) 58, 113, 211, 232, 274, 301
CviKI_1 RGCY 6 cut(s) 58, 113, 211, 232, 274, 301
CviQI GTAC 1 cut(s) 5
DdeI CTNAG 1 cut(s) 297
DpnI GATC 1 cut(s) 147
DpnII GATC 1 cut(s) 145
DrdI GACNNNNNNGTC 1 cut(s) 266
DseDI GACNNNNNNGTC 1 cut(s) 266
Eco130I CCWWGG 1 cut(s) 264
Eco24I GRGCYC 1 cut(s) 276
EcoT14I CCWWGG 1 cut(s) 264
EcoT38I GRGCYC 1 cut(s) 276
ErhI CCWWGG 1 cut(s) 264
FaeI CATG 3 cut(s) 286, 306, 370
FaiI YATR 7 cut(s) 32, 153, 189, 284, 304, 342, 368
FatI CATG 3 cut(s) 282, 302, 366
Fnu4HI GCNGC 1 cut(s) 111
FriOI GRGCYC 1 cut(s) 276
Fsp4HI GCNGC 1 cut(s) 111
FspBI CTAG 1 cut(s) 171
GluI GCNGC 1 cut(s) 111
HaeIII GGCC 1 cut(s) 211
Hin1II CATG 3 cut(s) 286, 306, 370
HincII GTYRAC 1 cut(s) 28
HindII GTYRAC 1 cut(s) 28
HindIII AAGCTT 1 cut(s) 56
HinfI GANTC 1 cut(s) 332
Hpy166II GTNNAC 1 cut(s) 28
Hpy8I GTNNAC 1 cut(s) 28
HpyCH4III ACNGT 3 cut(s) 16, 130, 143
HpyCH4V TGCA 3 cut(s) 110, 183, 221
HpyF10VI GCNNNNNNNGC 1 cut(s) 45
HpyF3I CTNAG 1 cut(s) 297
Hsp92II CATG 3 cut(s) 286, 306, 370
Kzo9I GATC 1 cut(s) 145
LpnPI CCDG 5 cut(s) 62, 120, 231, 309, 349
Lsp1109I GCAGC 1 cut(s) 122
LweI GCATC 1 cut(s) 97
MaeI CTAG 1 cut(s) 171
MaeIII GTNAC 1 cut(s) 130
MalI GATC 1 cut(s) 147
MboI GATC 1 cut(s) 145
MboII GAAGA 1 cut(s) 253
MhlI GDGCHC 1 cut(s) 276
MluCI AATT 1 cut(s) 159
MnlI CCTC 5 cut(s) 29, 45, 222, 225, 318
MwoI GCNNNNNNNGC 1 cut(s) 45
NdeII GATC 1 cut(s) 145
NlaIII CATG 3 cut(s) 286, 306, 370
NspI RCATGY 1 cut(s) 286
NspV TTCGAA 1 cut(s) 166
PfeI GAWTC 1 cut(s) 332
PkrI GCNGC 1 cut(s) 112
PspPI GGNCC 1 cut(s) 209
PstI CTGCAG 1 cut(s) 223
PstNI CAGNNNCTG 1 cut(s) 74
RsaI GTAC 1 cut(s) 6
RsaNI GTAC 1 cut(s) 5
SatI GCNGC 1 cut(s) 111
Sau3AI GATC 1 cut(s) 145
Sau96I GGNCC 1 cut(s) 209
SduI GDGCHC 1 cut(s) 276
SetI ASST 5 cut(s) 27, 60, 88, 270, 314
SfaNI GCATC 1 cut(s) 97
SfcI CTRYAG 1 cut(s) 219
SfuI TTCGAA 1 cut(s) 166
SmlI CTYRAG 2 cut(s) 20, 62
SmoI CTYRAG 2 cut(s) 20, 62
Sse9I AATT 1 cut(s) 159
SspMI CTAG 1 cut(s) 171
StyI CCWWGG 1 cut(s) 264
TaaI ACNGT 3 cut(s) 16, 130, 143
TaqI TCGA 2 cut(s) 166, 270
TasI AATT 1 cut(s) 159
TfiI GAWTC 1 cut(s) 332
TscAI CASTG 1 cut(s) 75
TseI GCWGC 1 cut(s) 110
TspDTI ATGAA 1 cut(s) 168
TspRI CASTG 1 cut(s) 75
XceI RCATGY 1 cut(s) 286
XcmI CCANNNNNNNNNTGG 1 cut(s) 309
XspI CTAG 1 cut(s) 171
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.