FvH4_1g12761

Heavy-metal-associated domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0001393
Physical Location & Seq
Reverse (-)
7041342 .. 7042527
1186 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g12761.t1

Sequence Viewer

Length: 375 bp
ATGGCACAAAGGACTGTCCTCAAGGTTGATATAACATGCCTAAAATGCAAAAAGAAGCTTCTCAAGGCAGTAACTGGCCTAGAAGGTGTGGATAAAATTGAAGTTGATGCAGCCAAGGGAACTTTGACAGTAACAGGAAATGCAGACCCTTATGACATAATAGTCCGGTGCAGAAGAGCCGGCAAGTTTGCTGAGGTAGTGACCGTCGGGCCTCCTCCGGCTCCAACTAAACCGAAAGAAGATGAGAAAAAGAAGCAGGAGGAGAAGAAACCAGTAGTCCAAAAGGATCAACTTCCCTATTATATGTATCAACCCCATATTATCATGATGCAGCCCCCTTGTTACGAGCCGAACCCGTCCTGCTCTATCATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HMA

Protein Analysis

125

Amino Acids

13.86

Weight (kDa)

9.07

Isoelectric Point (pI)

39.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 7 - 53 1e-11 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 161
AclWI GGATC 1 cut(s) 294
AgsI TTSAA 1 cut(s) 101
AluBI AGCT 1 cut(s) 58
AluI AGCT 1 cut(s) 58
AlwI GGATC 1 cut(s) 294
AlwNI CAGNNNCTG 1 cut(s) 74
AoxI GGCC 2 cut(s) 76, 209
ApeKI GCWGC 2 cut(s) 110, 331
AspS9I GGNCC 1 cut(s) 209
BbvCI CCTCAGC 1 cut(s) 192
BbvI GCAGC 2 cut(s) 122, 343
BfaI CTAG 1 cut(s) 80
BisI GCNGC 2 cut(s) 111, 332
BlsI GCNGC 2 cut(s) 112, 333
BmgT120I GGNCC 1 cut(s) 209
BmiI GGNNCC 1 cut(s) 222
BmsI GCATC 2 cut(s) 97, 318
Bpu10I CCTNAGC 1 cut(s) 192
BpuEI CTTGAG 2 cut(s) 5, 47
BsaJI CCNNGG 1 cut(s) 114
BsaWI WCCGGW 1 cut(s) 165
Bse118I RCCGGY 1 cut(s) 179
Bse1I ACTGG 2 cut(s) 79, 272
BseDI CCNNGG 1 cut(s) 114
BseMII CTCAG 1 cut(s) 183
BseNI ACTGG 2 cut(s) 79, 272
BseRI GAGGAG 2 cut(s) 204, 275
BseXI GCAGC 2 cut(s) 122, 343
BsgI GTGCAG 1 cut(s) 190
BshFI GGCC 2 cut(s) 78, 211
BsiSI CCGG 3 cut(s) 166, 180, 218
BsnI GGCC 2 cut(s) 78, 211
Bsp143I GATC 1 cut(s) 286
BspANI GGCC 2 cut(s) 78, 211
BspCNI CTCAG 1 cut(s) 184
BspHI TCATGA 1 cut(s) 324
BspLI GGNNCC 1 cut(s) 222
BspPI GGATC 1 cut(s) 294
BspQI GCTCTTC 1 cut(s) 169
BsrFI RCCGGY 1 cut(s) 179
BsrI ACTGG 2 cut(s) 79, 272
BssAI RCCGGY 1 cut(s) 179
BssECI CCNNGG 1 cut(s) 114
BssMI GATC 1 cut(s) 286
BssT1I CCWWGG 1 cut(s) 114
Bst4CI ACNGT 3 cut(s) 16, 130, 205
Bst6I CTCTTC 1 cut(s) 169
BstC8I GCNNGC 1 cut(s) 181
BstDEI CTNAG 1 cut(s) 192
BstKTI GATC 1 cut(s) 289
BstMBI GATC 1 cut(s) 286
BstMWI GCNNNNNNNGC 1 cut(s) 45
BstNSI RCATGY 1 cut(s) 39
BstV1I GCAGC 2 cut(s) 122, 343
BsuRI GGCC 2 cut(s) 78, 211
Cac8I GCNNGC 1 cut(s) 181
CaiI CAGNNNCTG 1 cut(s) 74
CciI TCATGA 1 cut(s) 324
Cfr10I RCCGGY 1 cut(s) 179
Cfr13I GGNCC 1 cut(s) 209
CviAII CATG 3 cut(s) 36, 325, 370
CviJI RGCY 8 cut(s) 58, 78, 113, 179, 211, 221, 334, 349
CviKI_1 RGCY 8 cut(s) 58, 78, 113, 179, 211, 221, 334, 349
DdeI CTNAG 1 cut(s) 192
DpnI GATC 1 cut(s) 288
DpnII GATC 1 cut(s) 286
DrdI GACNNNNNNGTC 1 cut(s) 161
DseDI GACNNNNNNGTC 1 cut(s) 161
Eam1104I CTCTTC 1 cut(s) 169
EarI CTCTTC 1 cut(s) 169
Eco130I CCWWGG 1 cut(s) 114
EcoT14I CCWWGG 1 cut(s) 114
ErhI CCWWGG 1 cut(s) 114
FaeI CATG 3 cut(s) 39, 328, 373
FaiI YATR 9 cut(s) 32, 37, 153, 158, 303, 305, 318, 326, 371
FatI CATG 3 cut(s) 35, 324, 369
Fnu4HI GCNGC 2 cut(s) 111, 332
Fsp4HI GCNGC 2 cut(s) 111, 332
FspBI CTAG 1 cut(s) 80
GluI GCNGC 2 cut(s) 111, 332
HaeIII GGCC 2 cut(s) 78, 211
HapII CCGG 3 cut(s) 166, 180, 218
Hin1II CATG 3 cut(s) 39, 328, 373
HindIII AAGCTT 1 cut(s) 56
HpaII CCGG 3 cut(s) 166, 180, 218
Hpy188III TCNNGA 1 cut(s) 325
Hpy99I CGWCG 1 cut(s) 209
HpyAV CCTTC 1 cut(s) 77
HpyCH4III ACNGT 3 cut(s) 16, 130, 205
HpyCH4V TGCA 5 cut(s) 48, 110, 143, 171, 331
HpyF10VI GCNNNNNNNGC 1 cut(s) 45
HpyF3I CTNAG 1 cut(s) 192
Hsp92II CATG 3 cut(s) 39, 328, 373
KroI GCCGGC 1 cut(s) 179
KroNI GCCGGC 1 cut(s) 181
Kzo9I GATC 1 cut(s) 286
LguI GCTCTTC 1 cut(s) 169
LmnI GCTCC 1 cut(s) 226
LpnPI CCDG 7 cut(s) 60, 120, 179, 193, 231, 242, 285
Lsp1109I GCAGC 2 cut(s) 122, 343
LweI GCATC 2 cut(s) 97, 318
MaeI CTAG 1 cut(s) 80
MaeIII GTNAC 4 cut(s) 70, 130, 199, 341
MalI GATC 1 cut(s) 288
MboI GATC 1 cut(s) 286
MboII GAAGA 3 cut(s) 186, 251, 277
MluCI AATT 1 cut(s) 96
MmeI TCCRAC 1 cut(s) 248
MnlI CCTC 5 cut(s) 29, 187, 222, 225, 253
MroNI GCCGGC 1 cut(s) 179
MspI CCGG 3 cut(s) 166, 180, 218
MwoI GCNNNNNNNGC 1 cut(s) 45
NaeI GCCGGC 1 cut(s) 181
NdeII GATC 1 cut(s) 286
NgoMIV GCCGGC 1 cut(s) 179
NlaIII CATG 3 cut(s) 39, 328, 373
NlaIV GGNNCC 1 cut(s) 222
NmuCI GTSAC 1 cut(s) 199
NspI RCATGY 1 cut(s) 39
PagI TCATGA 1 cut(s) 324
PciSI GCTCTTC 1 cut(s) 169
PdiI GCCGGC 1 cut(s) 181
PkrI GCNGC 2 cut(s) 112, 333
PspN4I GGNNCC 1 cut(s) 222
PspPI GGNCC 1 cut(s) 209
PstNI CAGNNNCTG 1 cut(s) 74
SapI GCTCTTC 1 cut(s) 169
SatI GCNGC 2 cut(s) 111, 332
Sau3AI GATC 1 cut(s) 286
Sau96I GGNCC 1 cut(s) 209
SetI ASST 4 cut(s) 27, 60, 88, 198
SfaNI GCATC 2 cut(s) 97, 318
SmlI CTYRAG 2 cut(s) 20, 62
SmoI CTYRAG 2 cut(s) 20, 62
Sse9I AATT 1 cut(s) 96
SspMI CTAG 1 cut(s) 80
StyI CCWWGG 1 cut(s) 114
TaaI ACNGT 3 cut(s) 16, 130, 205
TasI AATT 1 cut(s) 96
TseFI GTSAC 1 cut(s) 199
TseI GCWGC 2 cut(s) 110, 331
Tsp45I GTSAC 1 cut(s) 199
XceI RCATGY 1 cut(s) 39
XspI CTAG 1 cut(s) 80
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.