pycom02g11240

Heavy-metal-associated domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0001393
Physical Location & Seq
Reverse (-)
7962566 .. 7963306
741 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g11240.1

Sequence Viewer

Length: 375 bp
ATGGTACAAAGGACTGTTCTCAAGGTTGATATACATTGCCAAAAATGCCAGAGGAAGCTTCTCAAGGCAGTGTCTGTCCTAGAAGGTGTGGATAAACTTGAAGTTGATGCAGCCAAGGGAACTTTGACGGTAACAGGCGACTGTGATCCCTATGAAATAATTGTTCGAACTAGAAAAGCATGCAAATATGCCGAAGTAGTCAGTATTGGGCCTCCTCCTAAACCTCCAGAAAAGAAGCCCGAACAGACGAAACCTAAAGAGAAAGACCCGAAGCCCTATCCCATGCCCTATTATTGCCCTGAGCCATGTCAAAGGTGGGGCATTGTGAGGGTGGATCCCTGTTATGACCACAACCCTTCCTGCTCTATCATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HMA

Protein Analysis

125

Amino Acids

14.04

Weight (kDa)

8.75

Isoelectric Point (pI)

29.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 7 - 55 1.5e-11 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 3 cut(s) 140, 329, 342
AfaI GTAC 1 cut(s) 6
AgsI TTSAA 1 cut(s) 101
AluBI AGCT 1 cut(s) 58
AluI AGCT 1 cut(s) 58
AlwI GGATC 3 cut(s) 140, 329, 342
AlwNI CAGNNNCTG 1 cut(s) 74
AoxI GGCC 1 cut(s) 209
ApeKI GCWGC 1 cut(s) 110
AspS9I GGNCC 1 cut(s) 209
AsuII TTCGAA 1 cut(s) 166
BamHI GGATCC 1 cut(s) 334
BbvI GCAGC 1 cut(s) 122
BfaI CTAG 2 cut(s) 80, 171
BisI GCNGC 1 cut(s) 111
BlsI GCNGC 1 cut(s) 112
BmgT120I GGNCC 1 cut(s) 209
BmiI GGNNCC 1 cut(s) 336
BmsI GCATC 1 cut(s) 97
BpmI CTGGAG 1 cut(s) 210
Bpu10I CCTNAGC 1 cut(s) 300
Bpu14I TTCGAA 1 cut(s) 166
BpuEI CTTGAG 2 cut(s) 5, 47
BsaJI CCNNGG 1 cut(s) 114
Bse3DI GCAATG 1 cut(s) 34
BseDI CCNNGG 1 cut(s) 114
BseMI GCAATG 1 cut(s) 34
BseMII CTCAG 1 cut(s) 291
BseRI GAGGAG 1 cut(s) 204
BseXI GCAGC 1 cut(s) 122
BshFI GGCC 1 cut(s) 211
BsnI GGCC 1 cut(s) 211
Bsp119I TTCGAA 1 cut(s) 166
Bsp143I GATC 2 cut(s) 145, 334
BspANI GGCC 1 cut(s) 211
BspCNI CTCAG 1 cut(s) 292
BspLI GGNNCC 1 cut(s) 336
BspPI GGATC 3 cut(s) 140, 329, 342
BspT104I TTCGAA 1 cut(s) 166
BsrDI GCAATG 1 cut(s) 34
BssECI CCNNGG 1 cut(s) 114
BssMI GATC 2 cut(s) 145, 334
BssT1I CCWWGG 1 cut(s) 114
Bst4CI ACNGT 3 cut(s) 16, 130, 143
BstBI TTCGAA 1 cut(s) 166
BstC8I GCNNGC 1 cut(s) 181
BstDEI CTNAG 1 cut(s) 300
BstKTI GATC 2 cut(s) 148, 337
BstMBI GATC 2 cut(s) 145, 334
BstMWI GCNNNNNNNGC 1 cut(s) 45
BstNSI RCATGY 1 cut(s) 183
BstV1I GCAGC 1 cut(s) 122
BstX2I RGATCY 1 cut(s) 334
BstYI RGATCY 1 cut(s) 334
BsuRI GGCC 1 cut(s) 211
BtsI GCAGTG 1 cut(s) 75
BtsIMutI CAGTG 1 cut(s) 75
Cac8I GCNNGC 1 cut(s) 181
CaiI CAGNNNCTG 1 cut(s) 74
Cfr13I GGNCC 1 cut(s) 209
Csp6I GTAC 1 cut(s) 5
CviAII CATG 4 cut(s) 180, 283, 306, 370
CviJI RGCY 6 cut(s) 58, 113, 211, 238, 274, 304
CviKI_1 RGCY 6 cut(s) 58, 113, 211, 238, 274, 304
CviQI GTAC 1 cut(s) 5
DdeI CTNAG 1 cut(s) 300
DpnI GATC 2 cut(s) 147, 336
DpnII GATC 2 cut(s) 145, 334
Eco130I CCWWGG 1 cut(s) 114
EcoT14I CCWWGG 1 cut(s) 114
ErhI CCWWGG 1 cut(s) 114
FaeI CATG 4 cut(s) 183, 286, 309, 373
FaiI YATR 8 cut(s) 32, 153, 181, 189, 284, 307, 345, 371
FatI CATG 4 cut(s) 179, 282, 305, 369
Fnu4HI GCNGC 1 cut(s) 111
Fsp4HI GCNGC 1 cut(s) 111
FspBI CTAG 2 cut(s) 80, 171
GluI GCNGC 1 cut(s) 111
GsuI CTGGAG 1 cut(s) 210
HaeIII GGCC 1 cut(s) 211
Hin1II CATG 4 cut(s) 183, 286, 309, 373
HindIII AAGCTT 1 cut(s) 56
Hpy188III TCNNGA 1 cut(s) 227
HpyAV CCTTC 2 cut(s) 77, 366
HpyCH4III ACNGT 3 cut(s) 16, 130, 143
HpyCH4V TGCA 2 cut(s) 110, 183
HpyF10VI GCNNNNNNNGC 1 cut(s) 45
HpyF3I CTNAG 1 cut(s) 300
Hsp92II CATG 4 cut(s) 183, 286, 309, 373
Kzo9I GATC 2 cut(s) 145, 334
LpnPI CCDG 5 cut(s) 62, 120, 240, 312, 352
Lsp1109I GCAGC 1 cut(s) 122
LweI GCATC 1 cut(s) 97
MaeI CTAG 2 cut(s) 80, 171
MaeIII GTNAC 1 cut(s) 130
MalI GATC 2 cut(s) 147, 336
MboI GATC 2 cut(s) 145, 334
MflI RGATCY 1 cut(s) 334
MluCI AATT 1 cut(s) 159
MnlI CCTC 5 cut(s) 45, 222, 225, 234, 321
MwoI GCNNNNNNNGC 1 cut(s) 45
NdeII GATC 2 cut(s) 145, 334
NlaIII CATG 4 cut(s) 183, 286, 309, 373
NlaIV GGNNCC 1 cut(s) 336
NspI RCATGY 1 cut(s) 183
NspV TTCGAA 1 cut(s) 166
PaeI GCATGC 1 cut(s) 183
PkrI GCNGC 1 cut(s) 112
PspN4I GGNNCC 1 cut(s) 336
PspPI GGNCC 1 cut(s) 209
PstNI CAGNNNCTG 1 cut(s) 74
PsuI RGATCY 1 cut(s) 334
RsaI GTAC 1 cut(s) 6
RsaNI GTAC 1 cut(s) 5
SatI GCNGC 1 cut(s) 111
Sau3AI GATC 2 cut(s) 145, 334
Sau96I GGNCC 1 cut(s) 209
SetI ASST 6 cut(s) 27, 60, 88, 226, 256, 317
SfaNI GCATC 1 cut(s) 97
SfuI TTCGAA 1 cut(s) 166
SmlI CTYRAG 2 cut(s) 20, 62
SmoI CTYRAG 2 cut(s) 20, 62
SphI GCATGC 1 cut(s) 183
Sse9I AATT 1 cut(s) 159
SspMI CTAG 2 cut(s) 80, 171
StyI CCWWGG 1 cut(s) 114
TaaI ACNGT 3 cut(s) 16, 130, 143
TaqI TCGA 1 cut(s) 166
TasI AATT 1 cut(s) 159
TscAI CASTG 1 cut(s) 75
TseI GCWGC 1 cut(s) 110
TspDTI ATGAA 1 cut(s) 168
TspRI CASTG 1 cut(s) 75
XceI RCATGY 1 cut(s) 183
XcmI CCANNNNNNNNNTGG 1 cut(s) 312
XspI CTAG 2 cut(s) 80, 171
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.