AT5G66860

Ribosomal protein TL5, C-terminal domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0012519
Physical Location & Seq
Reverse (-)
26700986 .. 26702702
1717 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G66860.1

Sequence Viewer

Length: 750 bp
ATGGCGAAATGGTGGCGAGGCCTGAGATCCGTGACGGCGGAGGTGGCTGATCTTCCGGCTTCTTCATTCGGTCGATCGATTCGATGTATTCATCAGTATCAGACGATCCAGGCGATTCCACGTGAAGCTACGGGTCGAGGAGTATCGGCTCGGGATCGGACGATTGGTCGTATTCCCGCCGTCGTTTTCCCGCAATCGCTTCTAGATACTGATGCTTCGAAGCGAGGCGTGTCGAGGAAGCAGCTATTGACGGCGGATAAGAAGCAGATCAAGTCTATTATTGACTCTGTTGGTCTCCCATTCTTCTGTTCTACCACTTTCCAGCTTCAGATCCGAGCTGGTCAGGGTTCGTCGACGCTTGTTGAATCGGGTCGAGTACTTCCCCTCAAGGTTCATAGAGACGAAGAAACTGGAAAGATACTTAATTTAGTATTCGTATGGGCTGATGATGGGGAGAAGTTGAAGGTTGATGTTCCTGTTGTTTTCAAAGGGTTGGATCATTGTCCTGGTCTCCAGAAAGGGGGAAACCTGCGGACAATCCGAAGCACTCTGAAACTACTAGGGCCAGCTGAGCACATTCCATCTAAAATCGAAGTAGATGTGAGCAATCTGGACATTGAAGACAAAGTGTTATTGCAAGACGTTGTATTTCATCCATCATTGAAGCTACTGAGCAAGAATGAAACCATGCCCGTGTGTAAGATTGTCGCCACAAGCCCGGTGAAAGAACCAGAAGCTGTTCAAGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L25p Ribosomal_TL5_C

Protein Analysis

249

Amino Acids

27.34

Weight (kDa)

9.41

Isoelectric Point (pI)

42.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_TL5_C PF14693 153 - 238 2.6e-20 Ribosomal protein TL5, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012519)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G66860
fragaria_vesca FvH4_1g15520
malus_domestica MD02G1167000.v1.1 MD15G1279600.v1.1
prunus_persica Prupe.7G137800_v2.0.a1 Prupe.7G137800_v2.0.a1 Prupe.7G137800_v2.0.a1
pyrus_communis pycom02g13450
rosa_chinensis RchiOBHm_Chr2g0104801
rosa_laevigata RLG00000017358
rosa_multiflora Rmu_ssc0000030.1_g000001
rosa_rugosa Rorug02G0123000
rosa_samantha Rh2AG172900 Rh2BG180500 Rh2CG178100 Rh2DG179100
rosa_wichuraiana Rw0G008670 Rw2G013510 Rw2G013590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 537
AccI GTMKAC 1 cut(s) 353
AciI CCGC 5 cut(s) 38, 177, 191, 254, 532
AclWI GGATC 5 cut(s) 21, 100, 162, 325, 504
AcuI CTGAAG 1 cut(s) 311
AcvI CACGTG 1 cut(s) 122
AfaI GTAC 1 cut(s) 378
AfiI CCNNNNNNNGG 1 cut(s) 520
AgsI TTSAA 6 cut(s) 365, 463, 487, 620, 664, 743
AhdI GACNNNNNGTC 1 cut(s) 165
AjnI CCWGG 2 cut(s) 108, 505
AluBI AGCT 7 cut(s) 128, 244, 325, 338, 569, 667, 737
AluI AGCT 7 cut(s) 128, 244, 325, 338, 569, 667, 737
Alw21I GWGCWC 1 cut(s) 576
Alw26I GTCTC 3 cut(s) 299, 393, 515
AlwI GGATC 5 cut(s) 21, 100, 162, 325, 504
AlwNI CAGNNNCTG 1 cut(s) 737
Ama87I CYCGRG 1 cut(s) 150
AoxI GGCC 2 cut(s) 19, 563
ApeKI GCWGC 1 cut(s) 241
Asp700I GAANNNNTTC 1 cut(s) 738
AspS9I GGNCC 1 cut(s) 563
AsuC2I CCSGG 1 cut(s) 719
AsuHPI GGTGA 1 cut(s) 733
AsuII TTCGAA 1 cut(s) 218
AvaI CYCGRG 1 cut(s) 150
BbrPI CACGTG 1 cut(s) 122
BbsI GAAGAC 1 cut(s) 627
Bbv12I GWGCWC 1 cut(s) 576
BbvI GCAGC 1 cut(s) 253
BccI CCATC 3 cut(s) 443, 589, 664
BceAI ACGGC 3 cut(s) 51, 164, 267
BciT130I CCWGG 2 cut(s) 110, 507
BcnI CCSGG 1 cut(s) 719
BcoDI GTCTC 3 cut(s) 299, 393, 515
BfaI CTAG 2 cut(s) 203, 560
BfuAI ACCTGC 1 cut(s) 537
BisI GCNGC 1 cut(s) 242
BlpI GCTNAGC 1 cut(s) 570
BlsI GCNGC 1 cut(s) 243
BmcAI AGTACT 1 cut(s) 378
Bme1390I CCNGG 3 cut(s) 110, 507, 719
BmeRI GACNNNNNGTC 1 cut(s) 165
BmeT110I CYCGRG 1 cut(s) 150
BmgT120I GGNCC 1 cut(s) 563
BmrFI CCNGG 3 cut(s) 110, 507, 719
BmsI GCATC 1 cut(s) 202
BpiI GAAGAC 1 cut(s) 627
BpmI CTGGAG 1 cut(s) 497
Bpu1102I GCTNAGC 1 cut(s) 570
Bpu14I TTCGAA 1 cut(s) 218
BpuEI CTTGAG 1 cut(s) 371
BpuMI CCSGG 1 cut(s) 719
Bsa29I ATCGAT 1 cut(s) 77
BsaAI YACGTR 1 cut(s) 122
BsaI GGTCTC 2 cut(s) 299, 515
Bsc4I CCNNNNNNNGG 1 cut(s) 520
Bse1I ACTGG 1 cut(s) 415
BseBI CCWGG 2 cut(s) 110, 507
BseCI ATCGAT 1 cut(s) 77
BseGI GGATG 1 cut(s) 652
BseLI CCNNNNNNNGG 1 cut(s) 520
BseMII CTCAG 3 cut(s) 14, 561, 662
BseNI ACTGG 1 cut(s) 415
BseRI GAGGAG 1 cut(s) 153
BseXI GCAGC 1 cut(s) 253
Bsh1285I CGRYCG 2 cut(s) 73, 77
BshFI GGCC 2 cut(s) 21, 565
BshVI ATCGAT 1 cut(s) 77
BsiEI CGRYCG 2 cut(s) 73, 77
BsiHKAI GWGCWC 1 cut(s) 576
BsiHKCI CYCGRG 1 cut(s) 150
BsiSI CCGG 2 cut(s) 56, 719
BslI CCNNNNNNNGG 1 cut(s) 520
BsmAI GTCTC 3 cut(s) 299, 393, 515
BsmBI CGTCTC 1 cut(s) 393
BsnI GGCC 2 cut(s) 21, 565
Bso31I GGTCTC 2 cut(s) 299, 515
BsoBI CYCGRG 1 cut(s) 150
Bsp119I TTCGAA 1 cut(s) 218
Bsp1286I GDGCHC 1 cut(s) 576
Bsp143I GATC 8 cut(s) 26, 49, 74, 105, 154, 267, 330, 496
Bsp1720I GCTNAGC 1 cut(s) 570
BspACI CCGC 5 cut(s) 38, 177, 191, 254, 532
BspANI GGCC 2 cut(s) 21, 565
BspCNI CTCAG 3 cut(s) 15, 562, 663
BspDI ATCGAT 1 cut(s) 77
BspMI ACCTGC 1 cut(s) 537
BspPI GGATC 5 cut(s) 21, 100, 162, 325, 504
BspT104I TTCGAA 1 cut(s) 218
BspTNI GGTCTC 2 cut(s) 299, 515
BsrI ACTGG 1 cut(s) 415
BssMI GATC 8 cut(s) 26, 49, 74, 105, 154, 267, 330, 496
Bst2UI CCWGG 2 cut(s) 110, 507
BstBAI YACGTR 1 cut(s) 122
BstBI TTCGAA 1 cut(s) 218
BstC8I GCNNGC 1 cut(s) 567
BstDEI CTNAG 3 cut(s) 23, 570, 671
BstF5I GGATG 1 cut(s) 652
BstKTI GATC 8 cut(s) 29, 52, 77, 108, 157, 270, 333, 499
BstMAI GTCTC 3 cut(s) 299, 393, 515
BstMBI GATC 8 cut(s) 26, 49, 74, 105, 154, 267, 330, 496
BstMCI CGRYCG 2 cut(s) 73, 77
BstMWI GCNNNNNNNGC 3 cut(s) 44, 571, 743
BstNI CCWGG 2 cut(s) 110, 507
BstSCI CCNGG 3 cut(s) 108, 505, 717
BstV1I GCAGC 1 cut(s) 253
BstV2I GAAGAC 1 cut(s) 627
BstX2I RGATCY 2 cut(s) 26, 330
BstYI RGATCY 2 cut(s) 26, 330
Bsu15I ATCGAT 1 cut(s) 77
BsuRI GGCC 2 cut(s) 21, 565
BsuTUI ATCGAT 1 cut(s) 77
BtsCI GGATG 1 cut(s) 652
BveI ACCTGC 1 cut(s) 537
Cac8I GCNNGC 1 cut(s) 567
CaiI CAGNNNCTG 1 cut(s) 737
Cfr13I GGNCC 1 cut(s) 563
ClaI ATCGAT 1 cut(s) 77
CseI GACGC 1 cut(s) 364
Csp6I GTAC 1 cut(s) 377
CviAII CATG 1 cut(s) 688
CviQI GTAC 1 cut(s) 377
DdeI CTNAG 3 cut(s) 23, 570, 671
DpnI GATC 8 cut(s) 28, 51, 76, 107, 156, 269, 332, 498
DpnII GATC 8 cut(s) 26, 49, 74, 105, 154, 267, 330, 496
DriI GACNNNNNGTC 1 cut(s) 165
Eam1105I GACNNNNNGTC 1 cut(s) 165
EciI GGCGGA 2 cut(s) 53, 269
Eco147I AGGCCT 1 cut(s) 21
Eco31I GGTCTC 2 cut(s) 299, 515
Eco57I CTGAAG 1 cut(s) 311
Eco72I CACGTG 1 cut(s) 122
Eco88I CYCGRG 1 cut(s) 150
EcoRII CCWGG 2 cut(s) 108, 505
Esp3I CGTCTC 1 cut(s) 393
FaeI CATG 1 cut(s) 691
FaiI YATR 4 cut(s) 396, 439, 689, 748
FatI CATG 1 cut(s) 687
FauI CCCGC 2 cut(s) 184, 198
FblI GTMKAC 1 cut(s) 353
Fnu4HI GCNGC 1 cut(s) 242
FokI GGATG 1 cut(s) 639
Fsp4HI GCNGC 1 cut(s) 242
FspBI CTAG 2 cut(s) 203, 560
GluI GCNGC 1 cut(s) 242
GsuI CTGGAG 1 cut(s) 497
HaeIII GGCC 2 cut(s) 21, 565
HapII CCGG 2 cut(s) 56, 719
HgaI GACGC 1 cut(s) 364
Hin1II CATG 1 cut(s) 691
HincII GTYRAC 1 cut(s) 354
HindII GTYRAC 1 cut(s) 354
HinfI GANTC 4 cut(s) 79, 115, 284, 365
HpaII CCGG 2 cut(s) 56, 719
HphI GGTGA 1 cut(s) 733
Hpy166II GTNNAC 1 cut(s) 354
Hpy188I TCNGA 6 cut(s) 102, 159, 330, 335, 542, 552
Hpy188III TCNNGA 4 cut(s) 152, 203, 514, 611
Hpy8I GTNNAC 1 cut(s) 354
Hpy99I CGWCG 3 cut(s) 185, 355, 358
HpyAV CCTTC 1 cut(s) 457
HpyCH4IV ACGT 2 cut(s) 121, 642
HpyCH4V TGCA 1 cut(s) 637
HpyF10VI GCNNNNNNNGC 3 cut(s) 44, 571, 743
HpyF3I CTNAG 3 cut(s) 23, 570, 671
HpySE526I ACGT 2 cut(s) 121, 642
Hsp92II CATG 1 cut(s) 691
Kzo9I GATC 8 cut(s) 26, 49, 74, 105, 154, 267, 330, 496
Lsp1109I GCAGC 1 cut(s) 253
LweI GCATC 1 cut(s) 202
MaeI CTAG 2 cut(s) 203, 560
MaeII ACGT 2 cut(s) 121, 642
MaeIII GTNAC 1 cut(s) 31
MalI GATC 8 cut(s) 28, 51, 76, 107, 156, 269, 332, 498
MboI GATC 8 cut(s) 26, 49, 74, 105, 154, 267, 330, 496
MboII GAAGA 5 cut(s) 44, 54, 295, 416, 632
MflI RGATCY 2 cut(s) 26, 330
MhlI GDGCHC 1 cut(s) 576
MluCI AATT 1 cut(s) 424
MlyI GAGTC 1 cut(s) 278
MmeI TCCRAC 1 cut(s) 474
MnlI CCTC 6 cut(s) 11, 34, 131, 218, 228, 395
MroXI GAANNNNTTC 1 cut(s) 738
MseI TTAA 1 cut(s) 423
MslI CAYNNNNRTG 1 cut(s) 692
MspA1I CMGCKG 1 cut(s) 569
MspI CCGG 2 cut(s) 56, 719
MspR9I CCNGG 3 cut(s) 110, 507, 719
MvaI CCWGG 2 cut(s) 110, 507
MwoI GCNNNNNNNGC 3 cut(s) 44, 571, 743
NciI CCSGG 1 cut(s) 719
NdeII GATC 8 cut(s) 26, 49, 74, 105, 154, 267, 330, 496
NlaIII CATG 1 cut(s) 691
NmuCI GTSAC 1 cut(s) 31
NspV TTCGAA 1 cut(s) 218
PceI AGGCCT 1 cut(s) 21
PcsI WCGNNNNNNNCGW 2 cut(s) 79, 110
PdmI GAANNNNTTC 1 cut(s) 738
PfeI GAWTC 3 cut(s) 79, 115, 365
PkrI GCNGC 1 cut(s) 243
Ple19I CGATCG 1 cut(s) 77
PleI GAGTC 1 cut(s) 278
PmaCI CACGTG 1 cut(s) 122
PmlI CACGTG 1 cut(s) 122
PpsI GAGTC 1 cut(s) 278
Ppu21I YACGTR 1 cut(s) 122
Psp6I CCWGG 2 cut(s) 108, 505
PspCI CACGTG 1 cut(s) 122
PspGI CCWGG 2 cut(s) 108, 505
PspPI GGNCC 1 cut(s) 563
PstNI CAGNNNCTG 1 cut(s) 737
PsuI RGATCY 2 cut(s) 26, 330
PvuI CGATCG 1 cut(s) 77
PvuII CAGCTG 1 cut(s) 569
RsaI GTAC 1 cut(s) 378
RsaNI GTAC 1 cut(s) 377
RseI CAYNNNNRTG 1 cut(s) 692
SalI GTCGAC 1 cut(s) 352
SaqAI TTAA 1 cut(s) 423
SatI GCNGC 1 cut(s) 242
Sau3AI GATC 8 cut(s) 26, 49, 74, 105, 154, 267, 330, 496
Sau96I GGNCC 1 cut(s) 563
ScaI AGTACT 1 cut(s) 378
SchI GAGTC 1 cut(s) 278
ScrFI CCNGG 3 cut(s) 110, 507, 719
SduI GDGCHC 1 cut(s) 576
SfaNI GCATC 1 cut(s) 202
SfuI TTCGAA 1 cut(s) 218
SgrDI CGTCGACG 1 cut(s) 352
SmiMI CAYNNNNRTG 1 cut(s) 692
SmlI CTYRAG 1 cut(s) 386
SmoI CTYRAG 1 cut(s) 386
Sse9I AATT 1 cut(s) 424
SseBI AGGCCT 1 cut(s) 21
SsiI CCGC 5 cut(s) 38, 177, 191, 254, 532
SspMI CTAG 2 cut(s) 203, 560
StuI AGGCCT 1 cut(s) 21
StyD4I CCNGG 3 cut(s) 108, 505, 717
TaiI ACGT 2 cut(s) 124, 645
TaqI TCGA 9 cut(s) 73, 77, 82, 136, 218, 233, 353, 373, 591
TaqII GACCGA 1 cut(s) 59
TasI AATT 1 cut(s) 424
TatI WGTACW 1 cut(s) 376
TfiI GAWTC 3 cut(s) 79, 115, 365
Tru1I TTAA 1 cut(s) 423
Tru9I TTAA 1 cut(s) 423
TseFI GTSAC 1 cut(s) 31
TseI GCWGC 1 cut(s) 241
Tsp45I GTSAC 1 cut(s) 31
TspDTI ATGAA 5 cut(s) 54, 80, 383, 641, 696
TspGWI ACGGA 1 cut(s) 19
XbaI TCTAGA 1 cut(s) 202
XmiI GTMKAC 1 cut(s) 353
XmnI GAANNNNTTC 1 cut(s) 738
XspI CTAG 2 cut(s) 203, 560
ZrmI AGTACT 1 cut(s) 378
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.