FvH4_1g15520

Ribosomal protein TL5, C-terminal domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0012519
Physical Location & Seq
Forward (+)
8816641 .. 8818963
2323 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g15520.t1

Sequence Viewer

Length: 744 bp
ATGGCCAGGTGGTGGCGCTCAGCCGGCGGAGGCCTCCGCTCGCTTCTCTCCTCCCCAAACCCCTCTCTCCAATCCCAATCCTCTCTCTACCACACCATCCAAGCCATCCCCAGAGAGTTCACCGGAAACCGAATCTCCGCCAAAGACAGAGCCCAGGGTCGAATCCCCGCCGTCGTCTTCGCCCAGCCGCCGCCGGAAAACAGCCCCAAGCCCACCGGCCGATCCATCGCCAGAAAACACTTGCTGACGACTGAAAGAAAGCAGATTCAGGCCATTCTCAACTCCGTCGAGCTCCCCTATTTCTGCTCCACCACATTTCCGCTCCAGATCCGGGCCGGGTCCGGGTCCTCGGTTCTGCTTGAATCCGGGAAAGTCCTACCCGTCAAGATTCATAGAGATGAGGAGAGTGGGAAGATACTGAACTTGGTGTTTGTTTGGGCCGATGAGGGCTCCGAGTTGAAGGTGGATGTTCCGGTTACTTTCAAAGGTGAAGATGTTTGCCCTGGTCTCAAAAAAGGCGGTCATCTGAACAAAATTAGAACAAGTCTAAAGTACCTTTGCCCTGCCGAACACATTCCTCCCAAAATTGAGGTGGATATCAGCAATCTAGATATAGGAGATAGGGTGTGCCTTCCTGATGTTAAGGTTCATCCATCCCTGAAGCTTCTAAGCAAGAACGATGTTATACCCATATGTAAGATAGTTGCAACGAAGTTGGAAACCCCAGAACCGGCAGGAGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L25p Ribosomal_TL5_C

Protein Analysis

248

Amino Acids

27.02

Weight (kDa)

9.4

Isoelectric Point (pI)

50.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_TL5_C PF14693 153 - 237 6.7e-22 Ribosomal protein TL5, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012519)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G66860
fragaria_vesca FvH4_1g15520
malus_domestica MD02G1167000.v1.1 MD15G1279600.v1.1
prunus_persica Prupe.7G137800_v2.0.a1 Prupe.7G137800_v2.0.a1 Prupe.7G137800_v2.0.a1
pyrus_communis pycom02g13450
rosa_chinensis RchiOBHm_Chr2g0104801
rosa_laevigata RLG00000017358
rosa_multiflora Rmu_ssc0000030.1_g000001
rosa_rugosa Rorug02G0123000
rosa_samantha Rh2AG172900 Rh2BG180500 Rh2CG178100 Rh2DG179100
rosa_wichuraiana Rw0G008670 Rw2G013510 Rw2G013590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 12
AccBSI CCGCTC 2 cut(s) 39, 322
AciI CCGC 8 cut(s) 27, 37, 138, 168, 188, 191, 320, 519
AclWI GGATC 2 cut(s) 216, 322
AcoI YGGCCR 2 cut(s) 3, 217
AcuI CTGAAG 1 cut(s) 680
AfaI GTAC 1 cut(s) 554
AfiI CCNNNNNNNGG 4 cut(s) 12, 331, 342, 730
AgsI TTSAA 3 cut(s) 362, 460, 484
AjnI CCWGG 3 cut(s) 5, 153, 502
AluBI AGCT 2 cut(s) 292, 664
AluI AGCT 2 cut(s) 292, 664
Alw21I GWGCWC 1 cut(s) 294
Alw26I GTCTC 1 cut(s) 512
AlwI GGATC 2 cut(s) 216, 322
AoxI GGCC 6 cut(s) 3, 31, 217, 270, 333, 438
Asp700I GAANNNNTTC 1 cut(s) 573
AspLEI GCGC 1 cut(s) 18
AspS9I GGNCC 4 cut(s) 333, 339, 345, 438
AsuC2I CCSGG 4 cut(s) 332, 337, 343, 367
AsuHPI GGTGA 2 cut(s) 112, 500
AvaII GGWCC 2 cut(s) 339, 345
BalI TGGCCA 1 cut(s) 5
BanII GRGCYC 3 cut(s) 154, 294, 452
BbsI GAAGAC 1 cut(s) 169
Bbv12I GWGCWC 1 cut(s) 294
BccI CCATC 4 cut(s) 104, 113, 233, 661
BceAI ACGGC 1 cut(s) 155
BciT130I CCWGG 3 cut(s) 7, 155, 504
BcnI CCSGG 4 cut(s) 332, 337, 343, 367
BcoDI GTCTC 1 cut(s) 512
BfaI CTAG 1 cut(s) 608
BfoI RGCGCY 1 cut(s) 19
BisI GCNGC 2 cut(s) 188, 191
BlpI GCTNAGC 1 cut(s) 19
BlsI GCNGC 2 cut(s) 189, 192
Bme1390I CCNGG 7 cut(s) 7, 155, 332, 337, 343, 367, 504
Bme18I GGWCC 2 cut(s) 339, 345
BmgT120I GGNCC 4 cut(s) 333, 339, 345, 438
BmiI GGNNCC 3 cut(s) 340, 346, 451
BmrFI CCNGG 7 cut(s) 7, 155, 332, 337, 343, 367, 504
BpiI GAAGAC 1 cut(s) 169
BpmI CTGGAG 1 cut(s) 308
Bpu1102I GCTNAGC 1 cut(s) 19
BpuMI CCSGG 4 cut(s) 332, 337, 343, 367
BsaI GGTCTC 1 cut(s) 512
BsaJI CCNNGG 4 cut(s) 153, 154, 348, 502
BsaWI WCCGGW 2 cut(s) 122, 472
BsaXI ACNNNNNCTCC 4 cut(s) 119, 149, 609, 639
Bsc4I CCNNNNNNNGG 4 cut(s) 12, 331, 342, 730
Bse118I RCCGGY 3 cut(s) 23, 215, 730
BseBI CCWGG 3 cut(s) 7, 155, 504
BseDI CCNNGG 4 cut(s) 153, 154, 348, 502
BseGI GGATG 5 cut(s) 96, 105, 472, 649, 653
BseLI CCNNNNNNNGG 4 cut(s) 12, 331, 342, 730
BseMII CTCAG 1 cut(s) 33
BseRI GAGGAG 2 cut(s) 40, 416
BseX3I CGGCCG 1 cut(s) 217
BseYI CCCAGC 1 cut(s) 183
Bsh1285I CGRYCG 1 cut(s) 220
BshFI GGCC 6 cut(s) 5, 33, 219, 272, 335, 440
BsiEI CGRYCG 1 cut(s) 220
BsiHKAI GWGCWC 1 cut(s) 294
BslI CCNNNNNNNGG 4 cut(s) 12, 331, 342, 730
BsmAI GTCTC 1 cut(s) 512
BsnI GGCC 6 cut(s) 5, 33, 219, 272, 335, 440
Bso31I GGTCTC 1 cut(s) 512
Bsp1286I GDGCHC 3 cut(s) 154, 294, 452
Bsp143I GATC 2 cut(s) 221, 327
Bsp1720I GCTNAGC 1 cut(s) 19
BspACI CCGC 8 cut(s) 27, 37, 138, 168, 188, 191, 320, 519
BspANI GGCC 6 cut(s) 5, 33, 219, 272, 335, 440
BspCNI CTCAG 1 cut(s) 32
BspLI GGNNCC 3 cut(s) 340, 346, 451
BspPI GGATC 2 cut(s) 216, 322
BspTNI GGTCTC 1 cut(s) 512
BsrBI CCGCTC 2 cut(s) 39, 322
BsrFI RCCGGY 3 cut(s) 23, 215, 730
BssAI RCCGGY 3 cut(s) 23, 215, 730
BssECI CCNNGG 4 cut(s) 153, 154, 348, 502
BssMI GATC 2 cut(s) 221, 327
Bst2UI CCWGG 3 cut(s) 7, 155, 504
BstC8I GCNNGC 2 cut(s) 25, 41
BstDEI CTNAG 2 cut(s) 19, 668
BstF5I GGATG 5 cut(s) 96, 105, 472, 649, 653
BstH2I RGCGCY 1 cut(s) 19
BstHHI GCGC 1 cut(s) 18
BstKTI GATC 2 cut(s) 224, 330
BstMAI GTCTC 1 cut(s) 512
BstMBI GATC 2 cut(s) 221, 327
BstMCI CGRYCG 1 cut(s) 220
BstMWI GCNNNNNNNGC 1 cut(s) 24
BstNI CCWGG 3 cut(s) 7, 155, 504
BstSCI CCNGG 7 cut(s) 5, 153, 330, 335, 341, 365, 502
BstV2I GAAGAC 1 cut(s) 169
BstX2I RGATCY 1 cut(s) 327
BstYI RGATCY 1 cut(s) 327
BstZI CGGCCG 1 cut(s) 217
BsuRI GGCC 6 cut(s) 5, 33, 219, 272, 335, 440
BtgZI GCGATG 1 cut(s) 211
BtsCI GGATG 5 cut(s) 96, 105, 472, 649, 653
Cac8I GCNNGC 2 cut(s) 25, 41
CfoI GCGC 1 cut(s) 18
Cfr10I RCCGGY 3 cut(s) 23, 215, 730
Cfr13I GGNCC 4 cut(s) 333, 339, 345, 438
Csp6I GTAC 1 cut(s) 553
CviQI GTAC 1 cut(s) 553
DdeI CTNAG 2 cut(s) 19, 668
DpnI GATC 2 cut(s) 223, 329
DpnII GATC 2 cut(s) 221, 327
EaeI YGGCCR 2 cut(s) 3, 217
EagI CGGCCG 1 cut(s) 217
EciI GGCGGA 2 cut(s) 42, 127
Ecl136II GAGCTC 1 cut(s) 292
EclXI CGGCCG 1 cut(s) 217
Eco147I AGGCCT 1 cut(s) 33
Eco24I GRGCYC 3 cut(s) 154, 294, 452
Eco31I GGTCTC 1 cut(s) 512
Eco32I GATATC 1 cut(s) 598
Eco47I GGWCC 2 cut(s) 339, 345
Eco52I CGGCCG 1 cut(s) 217
Eco53kI GAGCTC 1 cut(s) 292
Eco57I CTGAAG 1 cut(s) 680
EcoICRI GAGCTC 1 cut(s) 292
EcoO109I RGGNCCY 1 cut(s) 345
EcoRII CCWGG 3 cut(s) 5, 153, 502
EcoRV GATATC 1 cut(s) 598
EcoT38I GRGCYC 3 cut(s) 154, 294, 452
FaiI YATR 5 cut(s) 393, 614, 686, 692, 694
FauI CCCGC 1 cut(s) 175
FauNDI CATATG 1 cut(s) 692
Fnu4HI GCNGC 2 cut(s) 188, 191
FokI GGATG 5 cut(s) 83, 92, 479, 636, 640
FriOI GRGCYC 3 cut(s) 154, 294, 452
Fsp4HI GCNGC 2 cut(s) 188, 191
FspBI CTAG 1 cut(s) 608
GlaI GCGC 1 cut(s) 17
GluI GCNGC 2 cut(s) 188, 191
GsaI CCCAGC 1 cut(s) 187
GsuI CTGGAG 1 cut(s) 308
HaeII RGCGCY 1 cut(s) 19
HaeIII GGCC 6 cut(s) 5, 33, 219, 272, 335, 440
HhaI GCGC 1 cut(s) 18
Hin6I GCGC 1 cut(s) 16
HinP1I GCGC 1 cut(s) 16
HindIII AAGCTT 1 cut(s) 662
HinfI GANTC 5 cut(s) 132, 162, 265, 362, 388
HphI GGTGA 2 cut(s) 112, 500
Hpy166II GTNNAC 1 cut(s) 120
Hpy188I TCNGA 2 cut(s) 454, 528
Hpy188III TCNNGA 4 cut(s) 325, 385, 608, 635
Hpy8I GTNNAC 1 cut(s) 120
Hpy99I CGWCG 2 cut(s) 176, 290
HpyAV CCTTC 2 cut(s) 454, 641
HpyCH4V TGCA 1 cut(s) 707
HpyF10VI GCNNNNNNNGC 1 cut(s) 24
HpyF3I CTNAG 2 cut(s) 19, 668
HspAI GCGC 1 cut(s) 16
KroI GCCGGC 1 cut(s) 23
KroNI GCCGGC 1 cut(s) 25
Kzo9I GATC 2 cut(s) 221, 327
LmnI GCTCC 4 cut(s) 297, 311, 327, 455
MaeI CTAG 1 cut(s) 608
MaeIII GTNAC 1 cut(s) 475
MalI GATC 2 cut(s) 223, 329
MbiI CCGCTC 2 cut(s) 39, 322
MboI GATC 2 cut(s) 221, 327
MboII GAAGA 3 cut(s) 169, 424, 503
MflI RGATCY 1 cut(s) 327
MhlI GDGCHC 3 cut(s) 154, 294, 452
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 2 cut(s) 534, 585
MluNI TGGCCA 1 cut(s) 5
MmeI TCCRAC 1 cut(s) 696
Mox20I TGGCCA 1 cut(s) 5
MroNI GCCGGC 1 cut(s) 23
MroXI GAANNNNTTC 1 cut(s) 573
MscI TGGCCA 1 cut(s) 5
MseI TTAA 1 cut(s) 642
MslI CAYNNNNRTG 1 cut(s) 396
Msp20I TGGCCA 1 cut(s) 5
MspR9I CCNGG 7 cut(s) 7, 155, 332, 337, 343, 367, 504
MvaI CCWGG 3 cut(s) 7, 155, 504
MwoI GCNNNNNNNGC 1 cut(s) 24
NaeI GCCGGC 1 cut(s) 25
NciI CCSGG 4 cut(s) 332, 337, 343, 367
NdeI CATATG 1 cut(s) 692
NdeII GATC 2 cut(s) 221, 327
NgoMIV GCCGGC 1 cut(s) 23
NlaIV GGNNCC 3 cut(s) 340, 346, 451
PasI CCCWGGG 1 cut(s) 154
PceI AGGCCT 1 cut(s) 33
PdiI GCCGGC 1 cut(s) 25
PdmI GAANNNNTTC 1 cut(s) 573
PfeI GAWTC 5 cut(s) 132, 162, 265, 362, 388
PflMI CCANNNNNTGG 1 cut(s) 12
PfoI TCCNGGA 1 cut(s) 365
PkrI GCNGC 2 cut(s) 189, 192
PpuMI RGGWCCY 1 cut(s) 345
Psp124BI GAGCTC 1 cut(s) 294
Psp5II RGGWCCY 1 cut(s) 345
Psp6I CCWGG 3 cut(s) 5, 153, 502
PspFI CCCAGC 1 cut(s) 183
PspGI CCWGG 3 cut(s) 5, 153, 502
PspN4I GGNNCC 3 cut(s) 340, 346, 451
PspPI GGNCC 4 cut(s) 333, 339, 345, 438
PspPPI RGGWCCY 1 cut(s) 345
PsuI RGATCY 1 cut(s) 327
RsaI GTAC 1 cut(s) 554
RsaNI GTAC 1 cut(s) 553
RseI CAYNNNNRTG 1 cut(s) 396
SacI GAGCTC 1 cut(s) 294
SaqAI TTAA 1 cut(s) 642
SatI GCNGC 2 cut(s) 188, 191
Sau3AI GATC 2 cut(s) 221, 327
Sau96I GGNCC 4 cut(s) 333, 339, 345, 438
ScrFI CCNGG 7 cut(s) 7, 155, 332, 337, 343, 367, 504
SduI GDGCHC 3 cut(s) 154, 294, 452
SetI ASST 8 cut(s) 11, 294, 465, 490, 558, 594, 648, 666
SinI GGWCC 2 cut(s) 339, 345
SmiMI CAYNNNNRTG 1 cut(s) 396
Sse9I AATT 2 cut(s) 534, 585
SseBI AGGCCT 1 cut(s) 33
SsiI CCGC 8 cut(s) 27, 37, 138, 168, 188, 191, 320, 519
SspMI CTAG 1 cut(s) 608
SstI GAGCTC 1 cut(s) 294
StuI AGGCCT 1 cut(s) 33
StyD4I CCNGG 7 cut(s) 5, 153, 330, 335, 341, 365, 502
TaqI TCGA 2 cut(s) 160, 288
TasI AATT 2 cut(s) 534, 585
TauI GCSGC 2 cut(s) 190, 193
TfiI GAWTC 5 cut(s) 132, 162, 265, 362, 388
Tru1I TTAA 1 cut(s) 642
Tru9I TTAA 1 cut(s) 642
TspDTI ATGAA 2 cut(s) 380, 638
TspGWI ACGGA 1 cut(s) 274
Van91I CCANNNNNTGG 1 cut(s) 12
VpaK11BI GGWCC 2 cut(s) 339, 345
XbaI TCTAGA 1 cut(s) 607
XcmI CCANNNNNNNNNTGG 1 cut(s) 589
XmnI GAANNNNTTC 1 cut(s) 573
XspI CTAG 1 cut(s) 608
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.