MD02G1167000.v1.1

50S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0012519
Physical Location & Seq
Forward (+)
14346379 .. 14348238
1860 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1167000.v1.1.491

Sequence Viewer

Length: 750 bp
ATGGCCAATCGGTGGCGCGCAGCCGGCGGCAACCTCCGCGCGGTTCTGCAAAATTCTAGCTTCGGACCGCAACCTCCATTCTCATCCTTCGCGGCTCTCTACCACACGATCCAAGCCATCCCTCGGGAGGTCACTGGCAACAGAGTCTCCGTCAAAGACAGAGCTCAAGGTCGAATTCCAGCCGTCGTGTTCTCCCAGCAGCTGAACCCTAGCAGTGCCGCCGCTCGACCGGTCGCCAGGAAGCACCTGCTGACCACCGAGAGGAAGCAGATTCAGGCCATTCTCAAGTCGGTAGAACTCCCGTTTTTCTGCTCCACTCGGTTCCCCCTCCAGATCCGAGCCGGGTCCGGGTCGTCGGATTTGCTCGAATCCGGAAATGTCATACCCATCAAGATTCACAGGGACCAGGAGAGTGGGAAGATTCTGAATTTGGTGTTTGTTTGGGCGGATGAGGGCTCCGAGCTGAAAGTTGATGTCCCTGTTGTTTTCAAAGGTGAAGATGTTTGTCCTGGTCTCAAAAAAGGTGGCCACCTGAACAAAATTAGAACAAGTCTAAGGTACCTTGGCCCAGCTGAGAACATTCCTTCTAAGATTGAGGTGGATGTAAGCAATCTAGATATAGGAGATAGGGTGTTCATTCCTGACATTGAGGTTCATCCTTCCATGAAGCTTCTAAGTAAGAACGAAACCATGCCCATTTGTAAGATAGTCGCAACAATGTTGGAGAACCCAGAATCTGCAGGAGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L25p Ribosomal_TL5_C

Protein Analysis

250

Amino Acids

27.24

Weight (kDa)

9.45

Isoelectric Point (pI)

40.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_TL5_C PF14693 155 - 238 8.1e-22 Ribosomal protein TL5, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012519)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G66860
fragaria_vesca FvH4_1g15520
malus_domestica MD02G1167000.v1.1 MD15G1279600.v1.1
prunus_persica Prupe.7G137800_v2.0.a1 Prupe.7G137800_v2.0.a1 Prupe.7G137800_v2.0.a1
pyrus_communis pycom02g13450
rosa_chinensis RchiOBHm_Chr2g0104801
rosa_laevigata RLG00000017358
rosa_multiflora Rmu_ssc0000030.1_g000001
rosa_rugosa Rorug02G0123000
rosa_samantha Rh2AG172900 Rh2BG180500 Rh2CG178100 Rh2DG179100
rosa_wichuraiana Rw0G008670 Rw2G013510 Rw2G013590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 255
Acc36I ACCTGC 1 cut(s) 255
Acc65I GGTACC 1 cut(s) 558
AccB1I GGYRCC 1 cut(s) 558
AccB7I CCANNNNNTGG 1 cut(s) 12
AccBSI CCGCTC 1 cut(s) 224
AccII CGCG 4 cut(s) 18, 39, 41, 92
AccIII TCCGGA 1 cut(s) 371
AciI CCGC 8 cut(s) 27, 37, 41, 68, 92, 219, 222, 446
AclWI GGATC 2 cut(s) 103, 328
AcoI YGGCCR 2 cut(s) 3, 526
AcsI RAATTY 3 cut(s) 52, 174, 427
AfaI GTAC 1 cut(s) 560
AfiI CCNNNNNNNGG 6 cut(s) 12, 40, 123, 127, 261, 348
AgeI ACCGGT 1 cut(s) 229
AgsI TTSAA 1 cut(s) 490
AjnI CCWGG 3 cut(s) 236, 405, 508
AluBI AGCT 6 cut(s) 60, 164, 202, 463, 572, 670
AluI AGCT 6 cut(s) 60, 164, 202, 463, 572, 670
Alw21I GWGCWC 1 cut(s) 166
Alw26I GTCTC 2 cut(s) 151, 518
AlwI GGATC 2 cut(s) 103, 328
AlwNI CAGNNNCTG 2 cut(s) 202, 737
Ama87I CYCGRG 1 cut(s) 123
Aor13HI TCCGGA 1 cut(s) 371
AoxI GGCC 4 cut(s) 3, 276, 526, 565
ApeKI GCWGC 2 cut(s) 20, 199
ApoI RAATTY 3 cut(s) 52, 174, 427
AsiGI ACCGGT 1 cut(s) 229
Asp718I GGTACC 1 cut(s) 558
AspLEI GCGC 3 cut(s) 18, 20, 41
AspS9I GGNCC 4 cut(s) 65, 345, 403, 566
AsuC2I CCSGG 2 cut(s) 343, 349
AsuHPI GGTGA 1 cut(s) 506
AvaI CYCGRG 1 cut(s) 123
AvaII GGWCC 3 cut(s) 65, 345, 403
BalI TGGCCA 2 cut(s) 5, 528
BanI GGYRCC 1 cut(s) 558
BanII GRGCYC 2 cut(s) 166, 458
Bbv12I GWGCWC 1 cut(s) 166
BbvI GCAGC 2 cut(s) 32, 211
BccI CCATC 2 cut(s) 125, 395
BceAI ACGGC 1 cut(s) 167
BciT130I CCWGG 3 cut(s) 238, 407, 510
BcnI CCSGG 2 cut(s) 343, 349
BcoDI GTCTC 2 cut(s) 151, 518
BfaI CTAG 3 cut(s) 57, 210, 614
BfmI CTRYAG 1 cut(s) 738
BfuAI ACCTGC 1 cut(s) 255
BisI GCNGC 6 cut(s) 21, 28, 93, 200, 219, 222
BlsI GCNGC 6 cut(s) 22, 29, 94, 201, 220, 223
Bme1390I CCNGG 5 cut(s) 238, 343, 349, 407, 510
Bme18I GGWCC 3 cut(s) 65, 345, 403
BmeT110I CYCGRG 1 cut(s) 123
BmgT120I GGNCC 4 cut(s) 65, 345, 403, 566
BmiI GGNNCC 5 cut(s) 323, 346, 404, 457, 560
BmrFI CCNGG 5 cut(s) 238, 343, 349, 407, 510
BpmI CTGGAG 1 cut(s) 314
BpuEI CTTGAG 2 cut(s) 150, 269
BpuMI CCSGG 2 cut(s) 343, 349
BsaI GGTCTC 1 cut(s) 518
BsaJI CCNNGG 2 cut(s) 122, 562
BsaWI WCCGGW 2 cut(s) 229, 371
BsaXI ACNNNNNCTCC 4 cut(s) 131, 161, 615, 645
Bsc4I CCNNNNNNNGG 6 cut(s) 12, 40, 123, 127, 261, 348
Bse118I RCCGGY 2 cut(s) 23, 229
Bse1I ACTGG 1 cut(s) 139
BseAI TCCGGA 1 cut(s) 371
BseBI CCWGG 3 cut(s) 238, 407, 510
BseDI CCNNGG 2 cut(s) 122, 562
BseGI GGATG 5 cut(s) 83, 117, 454, 607, 655
BseLI CCNNNNNNNGG 6 cut(s) 12, 40, 123, 127, 261, 348
BseMII CTCAG 1 cut(s) 564
BseNI ACTGG 1 cut(s) 139
BsePI GCGCGC 1 cut(s) 16
BseXI GCAGC 2 cut(s) 32, 211
BseYI CCCAGC 2 cut(s) 195, 568
Bsh1236I CGCG 4 cut(s) 18, 39, 41, 92
Bsh1285I CGRYCG 2 cut(s) 230, 234
BshFI GGCC 4 cut(s) 5, 278, 528, 567
BshNI GGYRCC 1 cut(s) 558
BshTI ACCGGT 1 cut(s) 229
BsiEI CGRYCG 2 cut(s) 230, 234
BsiHKAI GWGCWC 1 cut(s) 166
BsiHKCI CYCGRG 1 cut(s) 123
BsiSI CCGG 5 cut(s) 24, 230, 342, 348, 372
BslFI GGGAC 2 cut(s) 416, 461
BslI CCNNNNNNNGG 6 cut(s) 12, 40, 123, 127, 261, 348
BsmAI GTCTC 2 cut(s) 151, 518
BsmFI GGGAC 2 cut(s) 416, 461
BsnI GGCC 4 cut(s) 5, 278, 528, 567
Bso31I GGTCTC 1 cut(s) 518
BsoBI CYCGRG 1 cut(s) 123
Bsp1286I GDGCHC 2 cut(s) 166, 458
Bsp13I TCCGGA 1 cut(s) 371
Bsp143I GATC 2 cut(s) 108, 333
BspACI CCGC 8 cut(s) 27, 37, 41, 68, 92, 219, 222, 446
BspANI GGCC 4 cut(s) 5, 278, 528, 567
BspCNI CTCAG 1 cut(s) 565
BspEI TCCGGA 1 cut(s) 371
BspFNI CGCG 4 cut(s) 18, 39, 41, 92
BspLI GGNNCC 5 cut(s) 323, 346, 404, 457, 560
BspMAI CTGCAG 1 cut(s) 742
BspMI ACCTGC 1 cut(s) 255
BspPI GGATC 2 cut(s) 103, 328
BspT107I GGYRCC 1 cut(s) 558
BspTNI GGTCTC 1 cut(s) 518
BsrBI CCGCTC 1 cut(s) 224
BsrFI RCCGGY 2 cut(s) 23, 229
BsrI ACTGG 1 cut(s) 139
BssAI RCCGGY 2 cut(s) 23, 229
BssECI CCNNGG 2 cut(s) 122, 562
BssHII GCGCGC 1 cut(s) 16
BssMI GATC 2 cut(s) 108, 333
BssT1I CCWWGG 1 cut(s) 562
Bst2UI CCWGG 3 cut(s) 238, 407, 510
BstC8I GCNNGC 2 cut(s) 18, 25
BstDEI CTNAG 4 cut(s) 554, 573, 588, 674
BstF5I GGATG 5 cut(s) 83, 117, 454, 607, 655
BstFNI CGCG 4 cut(s) 18, 39, 41, 92
BstHHI GCGC 3 cut(s) 18, 20, 41
BstKTI GATC 2 cut(s) 111, 336
BstMAI GTCTC 2 cut(s) 151, 518
BstMBI GATC 2 cut(s) 108, 333
BstMCI CGRYCG 2 cut(s) 230, 234
BstMWI GCNNNNNNNGC 2 cut(s) 24, 36
BstNI CCWGG 3 cut(s) 238, 407, 510
BstSCI CCNGG 5 cut(s) 236, 341, 347, 405, 508
BstSFI CTRYAG 1 cut(s) 738
BstUI CGCG 4 cut(s) 18, 39, 41, 92
BstV1I GCAGC 2 cut(s) 32, 211
BstX2I RGATCY 1 cut(s) 333
BstXI CCANNNNNNTGG 1 cut(s) 413
BstYI RGATCY 1 cut(s) 333
BsuRI GGCC 4 cut(s) 5, 278, 528, 567
BtsCI GGATG 5 cut(s) 83, 117, 454, 607, 655
BtsI GCAGTG 1 cut(s) 220
BtsIMutI CAGTG 2 cut(s) 132, 220
BveI ACCTGC 1 cut(s) 255
Cac8I GCNNGC 2 cut(s) 18, 25
CaiI CAGNNNCTG 2 cut(s) 202, 737
CfoI GCGC 3 cut(s) 18, 20, 41
Cfr10I RCCGGY 2 cut(s) 23, 229
Cfr13I GGNCC 4 cut(s) 65, 345, 403, 566
CpoI CGGWCCG 1 cut(s) 65
Csp6I GTAC 1 cut(s) 559
CspAI ACCGGT 1 cut(s) 229
CspCI CAANNNNNGTGG 2 cut(s) 505, 540
CspI CGGWCCG 1 cut(s) 65
CviAII CATG 2 cut(s) 664, 691
CviQI GTAC 1 cut(s) 559
DdeI CTNAG 4 cut(s) 554, 573, 588, 674
DpnI GATC 2 cut(s) 110, 335
DpnII GATC 2 cut(s) 108, 333
EaeI YGGCCR 2 cut(s) 3, 526
EciI GGCGGA 1 cut(s) 461
Ecl136II GAGCTC 1 cut(s) 164
Eco130I CCWWGG 1 cut(s) 562
Eco24I GRGCYC 2 cut(s) 166, 458
Eco31I GGTCTC 1 cut(s) 518
Eco47I GGWCC 3 cut(s) 65, 345, 403
Eco53kI GAGCTC 1 cut(s) 164
Eco88I CYCGRG 1 cut(s) 123
EcoICRI GAGCTC 1 cut(s) 164
EcoRI GAATTC 1 cut(s) 174
EcoRII CCWGG 3 cut(s) 236, 405, 508
EcoT14I CCWWGG 1 cut(s) 562
EcoT38I GRGCYC 2 cut(s) 166, 458
ErhI CCWWGG 1 cut(s) 562
FaeI CATG 2 cut(s) 667, 694
FaiI YATR 4 cut(s) 383, 620, 665, 692
FaqI GGGAC 2 cut(s) 416, 461
FatI CATG 2 cut(s) 663, 690
Fnu4HI GCNGC 6 cut(s) 21, 28, 93, 200, 219, 222
FokI GGATG 5 cut(s) 70, 104, 461, 614, 642
FriOI GRGCYC 2 cut(s) 166, 458
Fsp4HI GCNGC 6 cut(s) 21, 28, 93, 200, 219, 222
FspBI CTAG 3 cut(s) 57, 210, 614
GlaI GCGC 3 cut(s) 17, 19, 40
GluI GCNGC 6 cut(s) 21, 28, 93, 200, 219, 222
GsaI CCCAGC 2 cut(s) 199, 572
GsuI CTGGAG 1 cut(s) 314
HaeIII GGCC 4 cut(s) 5, 278, 528, 567
HapII CCGG 5 cut(s) 24, 230, 342, 348, 372
HhaI GCGC 3 cut(s) 18, 20, 41
Hin1II CATG 2 cut(s) 667, 694
Hin6I GCGC 3 cut(s) 16, 18, 39
HinP1I GCGC 3 cut(s) 16, 18, 39
HindIII AAGCTT 1 cut(s) 668
HinfI GANTC 6 cut(s) 144, 271, 368, 394, 421, 734
HpaII CCGG 5 cut(s) 24, 230, 342, 348, 372
HphI GGTGA 1 cut(s) 506
Hpy188I TCNGA 5 cut(s) 65, 338, 358, 426, 460
Hpy188III TCNNGA 6 cut(s) 125, 331, 372, 391, 614, 641
Hpy99I CGWCG 2 cut(s) 188, 358
HpyAV CCTTC 3 cut(s) 97, 594, 669
HpyCH4V TGCA 2 cut(s) 49, 740
HpyF10VI GCNNNNNNNGC 2 cut(s) 24, 36
HpyF3I CTNAG 4 cut(s) 554, 573, 588, 674
Hsp92II CATG 2 cut(s) 667, 694
HspAI GCGC 3 cut(s) 16, 18, 39
Kpn2I TCCGGA 1 cut(s) 371
KpnI GGTACC 1 cut(s) 562
KroI GCCGGC 1 cut(s) 23
KroNI GCCGGC 1 cut(s) 25
Kzo9I GATC 2 cut(s) 108, 333
LmnI GCTCC 2 cut(s) 317, 461
Lsp1109I GCAGC 2 cut(s) 32, 211
MaeI CTAG 3 cut(s) 57, 210, 614
MaeIII GTNAC 1 cut(s) 130
MalI GATC 2 cut(s) 110, 335
MbiI CCGCTC 1 cut(s) 224
MboI GATC 2 cut(s) 108, 333
MboII GAAGA 2 cut(s) 430, 509
MflI RGATCY 1 cut(s) 333
MhlI GDGCHC 2 cut(s) 166, 458
MlsI TGGCCA 2 cut(s) 5, 528
MluCI AATT 4 cut(s) 52, 174, 427, 540
MluNI TGGCCA 2 cut(s) 5, 528
MlyI GAGTC 1 cut(s) 153
MmeI TCCRAC 2 cut(s) 336, 702
MnlI CCTC 9 cut(s) 44, 84, 121, 132, 255, 338, 445, 589, 643
Mox20I TGGCCA 2 cut(s) 5, 528
MroI TCCGGA 1 cut(s) 371
MroNI GCCGGC 1 cut(s) 23
MscI TGGCCA 2 cut(s) 5, 528
Msp20I TGGCCA 2 cut(s) 5, 528
MspA1I CMGCKG 2 cut(s) 202, 572
MspI CCGG 5 cut(s) 24, 230, 342, 348, 372
MspR9I CCNGG 5 cut(s) 238, 343, 349, 407, 510
MvaI CCWGG 3 cut(s) 238, 407, 510
MvnI CGCG 4 cut(s) 18, 39, 41, 92
MwoI GCNNNNNNNGC 2 cut(s) 24, 36
NaeI GCCGGC 1 cut(s) 25
NciI CCSGG 2 cut(s) 343, 349
NdeII GATC 2 cut(s) 108, 333
NgoMIV GCCGGC 1 cut(s) 23
NlaIII CATG 2 cut(s) 667, 694
NlaIV GGNNCC 5 cut(s) 323, 346, 404, 457, 560
NmuCI GTSAC 1 cut(s) 130
PaqCI CACCTGC 1 cut(s) 255
PauI GCGCGC 1 cut(s) 16
PdiI GCCGGC 1 cut(s) 25
PfeI GAWTC 5 cut(s) 271, 368, 394, 421, 734
PflMI CCANNNNNTGG 1 cut(s) 12
PinAI ACCGGT 1 cut(s) 229
PkrI GCNGC 6 cut(s) 22, 29, 94, 201, 220, 223
PleI GAGTC 1 cut(s) 152
PpsI GAGTC 1 cut(s) 152
Psp124BI GAGCTC 1 cut(s) 166
Psp6I CCWGG 3 cut(s) 236, 405, 508
PspFI CCCAGC 2 cut(s) 195, 568
PspGI CCWGG 3 cut(s) 236, 405, 508
PspN4I GGNNCC 5 cut(s) 323, 346, 404, 457, 560
PspPI GGNCC 4 cut(s) 65, 345, 403, 566
PstI CTGCAG 1 cut(s) 742
PstNI CAGNNNCTG 2 cut(s) 202, 737
PsuI RGATCY 1 cut(s) 333
PteI GCGCGC 1 cut(s) 16
PvuII CAGCTG 2 cut(s) 202, 572
RsaI GTAC 1 cut(s) 560
RsaNI GTAC 1 cut(s) 559
Rsr2I CGGWCCG 1 cut(s) 65
RsrII CGGWCCG 1 cut(s) 65
SacI GAGCTC 1 cut(s) 166
SatI GCNGC 6 cut(s) 21, 28, 93, 200, 219, 222
Sau3AI GATC 2 cut(s) 108, 333
Sau96I GGNCC 4 cut(s) 65, 345, 403, 566
SchI GAGTC 1 cut(s) 153
ScrFI CCNGG 5 cut(s) 238, 343, 349, 407, 510
SduI GDGCHC 2 cut(s) 166, 458
SfcI CTRYAG 1 cut(s) 738
SinI GGWCC 3 cut(s) 65, 345, 403
SmlI CTYRAG 2 cut(s) 165, 284
SmoI CTYRAG 2 cut(s) 165, 284
Sse9I AATT 4 cut(s) 52, 174, 427, 540
SsiI CCGC 8 cut(s) 27, 37, 41, 68, 92, 219, 222, 446
SspMI CTAG 3 cut(s) 57, 210, 614
SstI GAGCTC 1 cut(s) 166
StyD4I CCNGG 5 cut(s) 236, 341, 347, 405, 508
StyI CCWWGG 1 cut(s) 562
TaqI TCGA 3 cut(s) 172, 226, 366
TasI AATT 4 cut(s) 52, 174, 427, 540
TauI GCSGC 4 cut(s) 30, 95, 221, 224
TfiI GAWTC 5 cut(s) 271, 368, 394, 421, 734
TscAI CASTG 2 cut(s) 139, 220
TseFI GTSAC 1 cut(s) 130
TseI GCWGC 2 cut(s) 20, 199
Tsp45I GTSAC 1 cut(s) 130
TspDTI ATGAA 3 cut(s) 625, 644, 680
TspGWI ACGGA 1 cut(s) 139
TspRI CASTG 2 cut(s) 139, 220
Van91I CCANNNNNTGG 1 cut(s) 12
VpaK11BI GGWCC 3 cut(s) 65, 345, 403
XapI RAATTY 3 cut(s) 52, 174, 427
XbaI TCTAGA 1 cut(s) 613
XspI CTAG 3 cut(s) 57, 210, 614
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.