MD15G1279600.v1.1

50S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0012519
Physical Location & Seq
Forward (+)
24870428 .. 24871951
1524 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1279600.v1.1.491

Sequence Viewer

Length: 750 bp
ATGGCCAATCGGTGGCGCGCAGCCGGCAGCAACATCAGCGCGGTTCTGCAAAACCCCAGCTCCGGACCCCAACCTCCATTCTCATCCTTCGCGGCTATGTACCACACGATCCAAGCTATCCCTCGAGAAGTCACTGGAAACAGAGTCTCCGTCAAAGACAGAGCTCAAGGTCGAATCCCAGCCGTCGTGTTCTCTCAGCAGCTAAATCCTACCAGCGCCGCCGCTCGTCCGGTGTCCAGGAAGCACCTGCTGACCACCGAAAGGAAGCAGATTCAGGCCATTCTCAAGTCCGTAGAACTCCCATTTTTCTGCTCCACTCGGTTCCCCCTCCAGATCCGAGCCGGGTCCGGGTCATCGGATTTGCTCGAATCCGGAAATGTCATACCCATCAAGATTCATAGGGACGAAGAGAGTGGGAAGATTCTGAATTTGGTGTTTGTTTGGGCGGATGAGGGCTCGGAGCTGAAAGTTGACGTCCCTGTTGTTTTCAAAGGTGAAGATGTTTGTCCTGGTCTCAAGAAAGGTGGCCATCTGAACAAAATTAGAACTAGCCTAACGTACCTGGGACCAGCTGAGCACATTCCTACAAAGATTGAGGTGGATGTCAGCGATCTAGATATAGGAGATAGGGTGTTCGTTCCGGATGTTGAGGTTCATCCTTCCATGAAGCTTCTAAGTAAGAACGAAACCATGCCCATATGTAAGATAGTCGCAACAAATTTGGACAACCCAGAATCTGCACGAGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L25p Ribosomal_TL5_C

Protein Analysis

250

Amino Acids

27.22

Weight (kDa)

8.99

Isoelectric Point (pI)

39.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_TL5_C PF14693 155 - 239 1.4e-21 Ribosomal protein TL5, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012519)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G66860
fragaria_vesca FvH4_1g15520
malus_domestica MD02G1167000.v1.1 MD15G1279600.v1.1
prunus_persica Prupe.7G137800_v2.0.a1 Prupe.7G137800_v2.0.a1 Prupe.7G137800_v2.0.a1
pyrus_communis pycom02g13450
rosa_chinensis RchiOBHm_Chr2g0104801
rosa_laevigata RLG00000017358
rosa_multiflora Rmu_ssc0000030.1_g000001
rosa_rugosa Rorug02G0123000
rosa_samantha Rh2AG172900 Rh2BG180500 Rh2CG178100 Rh2DG179100
rosa_wichuraiana Rw0G008670 Rw2G013510 Rw2G013590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 255
AatII GACGTC 1 cut(s) 477
Acc36I ACCTGC 1 cut(s) 255
AccB7I CCANNNNNTGG 1 cut(s) 12
AccBSI CCGCTC 1 cut(s) 224
AccII CGCG 3 cut(s) 18, 41, 92
AccIII TCCGGA 3 cut(s) 62, 371, 640
AciI CCGC 5 cut(s) 41, 92, 219, 222, 446
AclWI GGATC 2 cut(s) 103, 328
AcoI YGGCCR 2 cut(s) 3, 526
AcsI RAATTY 2 cut(s) 427, 718
AcyI GRCGYC 1 cut(s) 474
AfaI GTAC 2 cut(s) 101, 560
AfiI CCNNNNNNNGG 4 cut(s) 12, 62, 261, 348
AgsI TTSAA 1 cut(s) 490
AjnI CCWGG 3 cut(s) 236, 508, 561
AluBI AGCT 7 cut(s) 60, 116, 164, 202, 463, 572, 670
AluI AGCT 7 cut(s) 60, 116, 164, 202, 463, 572, 670
Alw21I GWGCWC 2 cut(s) 166, 579
Alw26I GTCTC 2 cut(s) 151, 518
AlwI GGATC 2 cut(s) 103, 328
AlwNI CAGNNNCTG 1 cut(s) 737
Ama87I CYCGRG 1 cut(s) 123
Aor13HI TCCGGA 3 cut(s) 62, 371, 640
AoxI GGCC 3 cut(s) 3, 276, 526
ApeKI GCWGC 3 cut(s) 20, 27, 199
ApoI RAATTY 2 cut(s) 427, 718
AspLEI GCGC 4 cut(s) 18, 20, 41, 218
AspS9I GGNCC 3 cut(s) 65, 345, 566
AsuC2I CCSGG 2 cut(s) 343, 349
AsuHPI GGTGA 1 cut(s) 506
AvaI CYCGRG 1 cut(s) 123
AvaII GGWCC 3 cut(s) 65, 345, 566
BalI TGGCCA 2 cut(s) 5, 528
BanII GRGCYC 2 cut(s) 166, 458
BauI CACGAG 1 cut(s) 741
Bbv12I GWGCWC 2 cut(s) 166, 579
BbvI GCAGC 3 cut(s) 32, 39, 211
BccI CCATC 2 cut(s) 395, 537
BceAI ACGGC 1 cut(s) 167
BciT130I CCWGG 3 cut(s) 238, 510, 563
BcnI CCSGG 2 cut(s) 343, 349
BcoDI GTCTC 2 cut(s) 151, 518
BfaI CTAG 2 cut(s) 549, 614
BfoI RGCGCY 1 cut(s) 219
BfuAI ACCTGC 1 cut(s) 255
BisI GCNGC 6 cut(s) 21, 28, 93, 200, 219, 222
BlpI GCTNAGC 1 cut(s) 573
BlsI GCNGC 6 cut(s) 22, 29, 94, 201, 220, 223
Bme1390I CCNGG 5 cut(s) 238, 343, 349, 510, 563
Bme18I GGWCC 3 cut(s) 65, 345, 566
BmeT110I CYCGRG 1 cut(s) 123
BmgT120I GGNCC 3 cut(s) 65, 345, 566
BmiI GGNNCC 4 cut(s) 67, 323, 346, 567
BmrFI CCNGG 5 cut(s) 238, 343, 349, 510, 563
BpmI CTGGAG 1 cut(s) 314
Bpu1102I GCTNAGC 1 cut(s) 573
BpuEI CTTGAG 3 cut(s) 150, 269, 500
BpuMI CCSGG 2 cut(s) 343, 349
BsaHI GRCGYC 1 cut(s) 474
BsaI GGTCTC 1 cut(s) 518
BsaJI CCNNGG 1 cut(s) 562
BsaWI WCCGGW 4 cut(s) 62, 229, 371, 640
BsaXI ACNNNNNCTCC 6 cut(s) 44, 74, 131, 161, 615, 645
Bsc4I CCNNNNNNNGG 4 cut(s) 12, 62, 261, 348
Bse118I RCCGGY 1 cut(s) 23
Bse1I ACTGG 1 cut(s) 139
BseAI TCCGGA 3 cut(s) 62, 371, 640
BseBI CCWGG 3 cut(s) 238, 510, 563
BseDI CCNNGG 1 cut(s) 562
BseGI GGATG 5 cut(s) 83, 454, 607, 649, 655
BseLI CCNNNNNNNGG 4 cut(s) 12, 62, 261, 348
BseMII CTCAG 2 cut(s) 209, 564
BseNI ACTGG 1 cut(s) 139
BsePI GCGCGC 1 cut(s) 16
BseXI GCAGC 3 cut(s) 32, 39, 211
BseYI CCCAGC 2 cut(s) 56, 178
BsgI GTGCAG 1 cut(s) 723
Bsh1236I CGCG 3 cut(s) 18, 41, 92
BshFI GGCC 3 cut(s) 5, 278, 528
BsiHKAI GWGCWC 2 cut(s) 166, 579
BsiHKCI CYCGRG 1 cut(s) 123
BsiSI CCGG 7 cut(s) 24, 63, 230, 342, 348, 372, 641
BslFI GGGAC 3 cut(s) 416, 461, 579
BslI CCNNNNNNNGG 4 cut(s) 12, 62, 261, 348
BsmAI GTCTC 2 cut(s) 151, 518
BsmFI GGGAC 3 cut(s) 416, 461, 579
BsnI GGCC 3 cut(s) 5, 278, 528
Bso31I GGTCTC 1 cut(s) 518
BsoBI CYCGRG 1 cut(s) 123
Bsp1286I GDGCHC 3 cut(s) 166, 458, 579
Bsp13I TCCGGA 3 cut(s) 62, 371, 640
Bsp143I GATC 3 cut(s) 108, 333, 610
Bsp1720I GCTNAGC 1 cut(s) 573
BspACI CCGC 5 cut(s) 41, 92, 219, 222, 446
BspANI GGCC 3 cut(s) 5, 278, 528
BspCNI CTCAG 2 cut(s) 208, 565
BspEI TCCGGA 3 cut(s) 62, 371, 640
BspFNI CGCG 3 cut(s) 18, 41, 92
BspLI GGNNCC 4 cut(s) 67, 323, 346, 567
BspMI ACCTGC 1 cut(s) 255
BspPI GGATC 2 cut(s) 103, 328
BspTNI GGTCTC 1 cut(s) 518
BsrBI CCGCTC 1 cut(s) 224
BsrFI RCCGGY 1 cut(s) 23
BsrI ACTGG 1 cut(s) 139
BssAI RCCGGY 1 cut(s) 23
BssECI CCNNGG 1 cut(s) 562
BssHII GCGCGC 1 cut(s) 16
BssMI GATC 3 cut(s) 108, 333, 610
BssNI GRCGYC 1 cut(s) 474
BssSI CACGAG 1 cut(s) 741
Bst2BI CACGAG 1 cut(s) 741
Bst2UI CCWGG 3 cut(s) 238, 510, 563
Bst6I CTCTTC 1 cut(s) 402
BstACI GRCGYC 1 cut(s) 474
BstC8I GCNNGC 2 cut(s) 18, 25
BstDEI CTNAG 3 cut(s) 195, 573, 674
BstF5I GGATG 5 cut(s) 83, 454, 607, 649, 655
BstFNI CGCG 3 cut(s) 18, 41, 92
BstH2I RGCGCY 1 cut(s) 219
BstHHI GCGC 4 cut(s) 18, 20, 41, 218
BstKTI GATC 3 cut(s) 111, 336, 613
BstMAI GTCTC 2 cut(s) 151, 518
BstMBI GATC 3 cut(s) 108, 333, 610
BstMWI GCNNNNNNNGC 2 cut(s) 24, 36
BstNI CCWGG 3 cut(s) 238, 510, 563
BstSCI CCNGG 5 cut(s) 236, 341, 347, 508, 561
BstUI CGCG 3 cut(s) 18, 41, 92
BstV1I GCAGC 3 cut(s) 32, 39, 211
BstX2I RGATCY 1 cut(s) 333
BstYI RGATCY 1 cut(s) 333
BsuRI GGCC 3 cut(s) 5, 278, 528
BtsCI GGATG 5 cut(s) 83, 454, 607, 649, 655
BtsIMutI CAGTG 1 cut(s) 132
BveI ACCTGC 1 cut(s) 255
Cac8I GCNNGC 2 cut(s) 18, 25
CaiI CAGNNNCTG 1 cut(s) 737
CfoI GCGC 4 cut(s) 18, 20, 41, 218
Cfr10I RCCGGY 1 cut(s) 23
Cfr13I GGNCC 3 cut(s) 65, 345, 566
Csp6I GTAC 2 cut(s) 100, 559
CspCI CAANNNNNGTGG 2 cut(s) 505, 540
CviAII CATG 2 cut(s) 664, 691
CviQI GTAC 2 cut(s) 100, 559
DdeI CTNAG 3 cut(s) 195, 573, 674
DpnI GATC 3 cut(s) 110, 335, 612
DpnII GATC 3 cut(s) 108, 333, 610
EaeI YGGCCR 2 cut(s) 3, 526
Eam1104I CTCTTC 1 cut(s) 402
EarI CTCTTC 1 cut(s) 402
EciI GGCGGA 1 cut(s) 461
Ecl136II GAGCTC 1 cut(s) 164
Eco24I GRGCYC 2 cut(s) 166, 458
Eco31I GGTCTC 1 cut(s) 518
Eco47I GGWCC 3 cut(s) 65, 345, 566
Eco53kI GAGCTC 1 cut(s) 164
Eco88I CYCGRG 1 cut(s) 123
EcoICRI GAGCTC 1 cut(s) 164
EcoRII CCWGG 3 cut(s) 236, 508, 561
EcoT38I GRGCYC 2 cut(s) 166, 458
FaeI CATG 2 cut(s) 667, 694
FaiI YATR 8 cut(s) 98, 383, 399, 620, 665, 692, 698, 700
FaqI GGGAC 3 cut(s) 416, 461, 579
FatI CATG 2 cut(s) 663, 690
FauNDI CATATG 1 cut(s) 698
Fnu4HI GCNGC 6 cut(s) 21, 28, 93, 200, 219, 222
FokI GGATG 5 cut(s) 70, 461, 614, 642, 656
FriOI GRGCYC 2 cut(s) 166, 458
Fsp4HI GCNGC 6 cut(s) 21, 28, 93, 200, 219, 222
FspBI CTAG 2 cut(s) 549, 614
GlaI GCGC 4 cut(s) 17, 19, 40, 217
GluI GCNGC 6 cut(s) 21, 28, 93, 200, 219, 222
GsaI CCCAGC 2 cut(s) 60, 182
GsuI CTGGAG 1 cut(s) 314
HaeII RGCGCY 1 cut(s) 219
HaeIII GGCC 3 cut(s) 5, 278, 528
HapII CCGG 7 cut(s) 24, 63, 230, 342, 348, 372, 641
HhaI GCGC 4 cut(s) 18, 20, 41, 218
Hin1I GRCGYC 1 cut(s) 474
Hin1II CATG 2 cut(s) 667, 694
Hin6I GCGC 4 cut(s) 16, 18, 39, 216
HinP1I GCGC 4 cut(s) 16, 18, 39, 216
HincII GTYRAC 1 cut(s) 472
HindII GTYRAC 1 cut(s) 472
HindIII AAGCTT 1 cut(s) 668
HinfI GANTC 7 cut(s) 144, 174, 271, 368, 394, 421, 734
HpaII CCGG 7 cut(s) 24, 63, 230, 342, 348, 372, 641
HphI GGTGA 1 cut(s) 506
Hpy166II GTNNAC 1 cut(s) 472
Hpy188I TCNGA 5 cut(s) 338, 358, 426, 460, 534
Hpy188III TCNNGA 8 cut(s) 63, 125, 331, 372, 391, 517, 614, 641
Hpy8I GTNNAC 1 cut(s) 472
Hpy99I CGWCG 1 cut(s) 188
HpyAV CCTTC 2 cut(s) 97, 669
HpyCH4IV ACGT 2 cut(s) 474, 557
HpyCH4V TGCA 2 cut(s) 49, 740
HpyF10VI GCNNNNNNNGC 2 cut(s) 24, 36
HpyF3I CTNAG 3 cut(s) 195, 573, 674
HpySE526I ACGT 2 cut(s) 474, 557
Hsp92I GRCGYC 1 cut(s) 474
Hsp92II CATG 2 cut(s) 667, 694
HspAI GCGC 4 cut(s) 16, 18, 39, 216
Kpn2I TCCGGA 3 cut(s) 62, 371, 640
KroI GCCGGC 1 cut(s) 23
KroNI GCCGGC 1 cut(s) 25
Kzo9I GATC 3 cut(s) 108, 333, 610
LmnI GCTCC 3 cut(s) 65, 317, 460
Lsp1109I GCAGC 3 cut(s) 32, 39, 211
MaeI CTAG 2 cut(s) 549, 614
MaeII ACGT 2 cut(s) 474, 557
MaeIII GTNAC 1 cut(s) 130
MalI GATC 3 cut(s) 110, 335, 612
MbiI CCGCTC 1 cut(s) 224
MboI GATC 3 cut(s) 108, 333, 610
MboII GAAGA 3 cut(s) 419, 430, 509
MflI RGATCY 1 cut(s) 333
MhlI GDGCHC 3 cut(s) 166, 458, 579
MlsI TGGCCA 2 cut(s) 5, 528
MluCI AATT 3 cut(s) 427, 540, 718
MluNI TGGCCA 2 cut(s) 5, 528
MlyI GAGTC 1 cut(s) 153
MnlI CCTC 6 cut(s) 84, 132, 338, 445, 589, 643
Mox20I TGGCCA 2 cut(s) 5, 528
MroI TCCGGA 3 cut(s) 62, 371, 640
MroNI GCCGGC 1 cut(s) 23
MscI TGGCCA 2 cut(s) 5, 528
Msp20I TGGCCA 2 cut(s) 5, 528
MspA1I CMGCKG 1 cut(s) 572
MspI CCGG 7 cut(s) 24, 63, 230, 342, 348, 372, 641
MspR9I CCNGG 5 cut(s) 238, 343, 349, 510, 563
MvaI CCWGG 3 cut(s) 238, 510, 563
MvnI CGCG 3 cut(s) 18, 41, 92
MwoI GCNNNNNNNGC 2 cut(s) 24, 36
NaeI GCCGGC 1 cut(s) 25
NciI CCSGG 2 cut(s) 343, 349
NdeI CATATG 1 cut(s) 698
NdeII GATC 3 cut(s) 108, 333, 610
NgoMIV GCCGGC 1 cut(s) 23
NlaIII CATG 2 cut(s) 667, 694
NlaIV GGNNCC 4 cut(s) 67, 323, 346, 567
NmuCI GTSAC 1 cut(s) 130
PaeR7I CTCGAG 1 cut(s) 123
PaqCI CACCTGC 1 cut(s) 255
PauI GCGCGC 1 cut(s) 16
PdiI GCCGGC 1 cut(s) 25
PfeI GAWTC 6 cut(s) 174, 271, 368, 394, 421, 734
PflMI CCANNNNNTGG 1 cut(s) 12
PfoI TCCNGGA 1 cut(s) 236
PkrI GCNGC 6 cut(s) 22, 29, 94, 201, 220, 223
PleI GAGTC 1 cut(s) 152
PpsI GAGTC 1 cut(s) 152
Psp124BI GAGCTC 1 cut(s) 166
Psp6I CCWGG 3 cut(s) 236, 508, 561
PspFI CCCAGC 2 cut(s) 56, 178
PspGI CCWGG 3 cut(s) 236, 508, 561
PspN4I GGNNCC 4 cut(s) 67, 323, 346, 567
PspPI GGNCC 3 cut(s) 65, 345, 566
PstNI CAGNNNCTG 1 cut(s) 737
PsuI RGATCY 1 cut(s) 333
PteI GCGCGC 1 cut(s) 16
PvuII CAGCTG 1 cut(s) 572
RsaI GTAC 2 cut(s) 101, 560
RsaNI GTAC 2 cut(s) 100, 559
SacI GAGCTC 1 cut(s) 166
SatI GCNGC 6 cut(s) 21, 28, 93, 200, 219, 222
Sau3AI GATC 3 cut(s) 108, 333, 610
Sau96I GGNCC 3 cut(s) 65, 345, 566
SchI GAGTC 1 cut(s) 153
ScrFI CCNGG 5 cut(s) 238, 343, 349, 510, 563
SduI GDGCHC 3 cut(s) 166, 458, 579
Sfr274I CTCGAG 1 cut(s) 123
SinI GGWCC 3 cut(s) 65, 345, 566
SlaI CTCGAG 1 cut(s) 123
SmlI CTYRAG 4 cut(s) 123, 165, 284, 515
SmoI CTYRAG 4 cut(s) 123, 165, 284, 515
Sse9I AATT 3 cut(s) 427, 540, 718
SsiI CCGC 5 cut(s) 41, 92, 219, 222, 446
SspMI CTAG 2 cut(s) 549, 614
SstI GAGCTC 1 cut(s) 166
StyD4I CCNGG 5 cut(s) 236, 341, 347, 508, 561
TaiI ACGT 2 cut(s) 477, 560
TaqI TCGA 3 cut(s) 124, 172, 366
TasI AATT 3 cut(s) 427, 540, 718
TauI GCSGC 3 cut(s) 95, 221, 224
TfiI GAWTC 6 cut(s) 174, 271, 368, 394, 421, 734
TscAI CASTG 1 cut(s) 139
TseFI GTSAC 1 cut(s) 130
TseI GCWGC 3 cut(s) 20, 27, 199
Tsp45I GTSAC 1 cut(s) 130
TspDTI ATGAA 3 cut(s) 386, 644, 680
TspGWI ACGGA 2 cut(s) 139, 280
TspRI CASTG 1 cut(s) 139
Van91I CCANNNNNTGG 1 cut(s) 12
VpaK11BI GGWCC 3 cut(s) 65, 345, 566
XapI RAATTY 2 cut(s) 427, 718
XbaI TCTAGA 1 cut(s) 613
XhoI CTCGAG 1 cut(s) 123
XspI CTAG 2 cut(s) 549, 614
ZraI GACGTC 1 cut(s) 475
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.