pycom02g13450

50S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0012519
Physical Location & Seq
Forward (+)
9973046 .. 9975025
1980 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g13450.3

Sequence Viewer

Length: 1005 bp
ATGGTGTACACCTTTCTATTAGTTCATATTCACTTGTCAAATTTTGTTTGCATTCTTAAAATTAACGTTCAACTGTTAAATTCAGCAATCGCCCACATCCCCCAAATTTGGAGACCCAATAAGTCCATTATTCAACGAGCCCACCGCTCTGATAAGGCAAAGGTCATCTCTACCCAAAGGCCCAGACCCTTAAACCCCAAAACCCTCTGCGCAGAGACCAACACTCCCCTGCCCCCGCCCCACCCCAAAGCAAAAATGGCCAATCGGTGGCGCACAGCCGGCGGCAACTTCCGCGCGGTTCTGCAAAATTCTAGCTTCGGACCGCAACCTCCATTCTCATCATTCGCGGCTCTCTACCACACGATCCAAGCCATCCCTCGGGAGGTCACTGGCAACAGAGTCTCCGTCAAAGACAGAGATCAAGGTCGAATCCCAGCCGTCGTGTTCTCCCAGCAGCTGAACCCTAGCAGTGCCGCCGCTCGACCGGTCGCCAGGAAGCAGTTGCTGACTACCGAGAGGAAGCAGATTCAGGCCATTCTCAAGTCGGTAGAATTCCCATTTTTCTGCTCCACTCGGTTCCCCCTCCAGATCCGAGCGGGGTCCGGGTCGTCGGATTTGCTCGAATCCGGAAATGTCATACCCATCAAGATTCACAGGGACCAGGAGAGTGGGAAGATTCTGAATTTGGTGTTTGTTTGGGCGGATGAGGGCTCCGAGCTGAAAGTTGATGTCCCTGTTGTTTTCAAAGGTGAAGATGTTTGTCCTGGTCTCAAAAAAGGTGGCCACCTGAACAAAATTAGAACAAGTCTAAGGTACCTCGGCCCAGCTGAGAACATTCCTACTAAGATTGAGGTGGATGTCAGCAATCTAGATATAGGAGATAGGGTGTTTATTCCTGATGTCAAGGTTCATCCTTCCATGAAGCTTCTAAGTAAGAACGAAACCATGCCTATATGTAAGATAGTTGCAACAATGTTGGAGAACCCAGAATCTGCAGGAGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L25p Ribosomal_TL5_C

Protein Analysis

335

Amino Acids

37.03

Weight (kDa)

9.95

Isoelectric Point (pI)

37.36

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0012519)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G66860
fragaria_vesca FvH4_1g15520
malus_domestica MD02G1167000.v1.1 MD15G1279600.v1.1
prunus_persica Prupe.7G137800_v2.0.a1 Prupe.7G137800_v2.0.a1 Prupe.7G137800_v2.0.a1
pyrus_communis pycom02g13450
rosa_chinensis RchiOBHm_Chr2g0104801
rosa_laevigata RLG00000017358
rosa_multiflora Rmu_ssc0000030.1_g000001
rosa_rugosa Rorug02G0123000
rosa_samantha Rh2AG172900 Rh2BG180500 Rh2CG178100 Rh2DG179100
rosa_wichuraiana Rw0G008670 Rw2G013510 Rw2G013590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 211
Acc65I GGTACC 1 cut(s) 813
AccB1I GGYRCC 1 cut(s) 813
AccB7I CCANNNNNTGG 1 cut(s) 267
AccBSI CCGCTC 3 cut(s) 147, 479, 596
AccII CGCG 3 cut(s) 294, 296, 347
AccIII TCCGGA 1 cut(s) 626
AclI AACGTT 1 cut(s) 66
AclWI GGATC 2 cut(s) 358, 583
AcoI YGGCCR 2 cut(s) 258, 781
AcsI RAATTY 6 cut(s) 40, 79, 105, 307, 551, 682
AfaI GTAC 2 cut(s) 8, 815
AfiI CCNNNNNNNGG 4 cut(s) 108, 267, 378, 382
AgeI ACCGGT 1 cut(s) 484
AgsI TTSAA 3 cut(s) 71, 134, 745
AjnI CCWGG 3 cut(s) 491, 660, 763
AluBI AGCT 5 cut(s) 315, 457, 718, 827, 925
AluI AGCT 5 cut(s) 315, 457, 718, 827, 925
Alw26I GTCTC 4 cut(s) 106, 209, 406, 773
AlwI GGATC 2 cut(s) 358, 583
AlwNI CAGNNNCTG 3 cut(s) 457, 505, 992
Ama87I CYCGRG 1 cut(s) 378
Aor13HI TCCGGA 1 cut(s) 626
AoxI GGCC 5 cut(s) 179, 258, 531, 781, 820
ApeKI GCWGC 1 cut(s) 454
ApoI RAATTY 6 cut(s) 40, 79, 105, 307, 551, 682
AsiGI ACCGGT 1 cut(s) 484
Asp718I GGTACC 1 cut(s) 813
AspLEI GCGC 3 cut(s) 212, 273, 296
AspS9I GGNCC 5 cut(s) 180, 320, 600, 658, 821
AsuC2I CCSGG 1 cut(s) 604
AsuHPI GGTGA 1 cut(s) 761
AvaI CYCGRG 1 cut(s) 378
AvaII GGWCC 3 cut(s) 320, 600, 658
BalI TGGCCA 2 cut(s) 260, 783
BanI GGYRCC 1 cut(s) 813
BanII GRGCYC 2 cut(s) 142, 713
BbvI GCAGC 1 cut(s) 466
BccI CCATC 2 cut(s) 380, 650
BceAI ACGGC 1 cut(s) 422
BciT130I CCWGG 3 cut(s) 493, 662, 765
BcnI CCSGG 1 cut(s) 604
BcoDI GTCTC 4 cut(s) 106, 209, 406, 773
BfaI CTAG 3 cut(s) 312, 465, 869
BfmI CTRYAG 1 cut(s) 993
BisI GCNGC 5 cut(s) 283, 348, 455, 474, 477
BlsI GCNGC 5 cut(s) 284, 349, 456, 475, 478
Bme1390I CCNGG 4 cut(s) 493, 604, 662, 765
Bme18I GGWCC 3 cut(s) 320, 600, 658
BmeT110I CYCGRG 1 cut(s) 378
BmgT120I GGNCC 5 cut(s) 180, 320, 600, 658, 821
BmiI GGNNCC 5 cut(s) 578, 601, 659, 712, 815
BmrFI CCNGG 4 cut(s) 493, 604, 662, 765
BpmI CTGGAG 1 cut(s) 569
BpuEI CTTGAG 1 cut(s) 524
BpuMI CCSGG 1 cut(s) 604
BsaI GGTCTC 3 cut(s) 106, 209, 773
BsaJI CCNNGG 2 cut(s) 377, 817
BsaWI WCCGGW 2 cut(s) 484, 626
BsaXI ACNNNNNCTCC 6 cut(s) 208, 238, 386, 416, 870, 900
Bsc4I CCNNNNNNNGG 4 cut(s) 108, 267, 378, 382
Bse118I RCCGGY 2 cut(s) 278, 484
Bse1I ACTGG 1 cut(s) 394
BseAI TCCGGA 1 cut(s) 626
BseBI CCWGG 3 cut(s) 493, 662, 765
BseDI CCNNGG 2 cut(s) 377, 817
BseGI GGATG 5 cut(s) 96, 372, 709, 862, 910
BseLI CCNNNNNNNGG 4 cut(s) 108, 267, 378, 382
BseMII CTCAG 1 cut(s) 819
BseNI ACTGG 1 cut(s) 394
BseXI GCAGC 1 cut(s) 466
BseYI CCCAGC 3 cut(s) 433, 450, 823
Bsh1236I CGCG 3 cut(s) 294, 296, 347
Bsh1285I CGRYCG 2 cut(s) 485, 489
BshFI GGCC 5 cut(s) 181, 260, 533, 783, 822
BshNI GGYRCC 1 cut(s) 813
BshTI ACCGGT 1 cut(s) 484
BsiEI CGRYCG 2 cut(s) 485, 489
BsiHKCI CYCGRG 1 cut(s) 378
BsiSI CCGG 4 cut(s) 279, 485, 603, 627
BslFI GGGAC 2 cut(s) 671, 716
BslI CCNNNNNNNGG 4 cut(s) 108, 267, 378, 382
BsmAI GTCTC 4 cut(s) 106, 209, 406, 773
BsmFI GGGAC 2 cut(s) 671, 716
BsmI GAATGC 1 cut(s) 51
BsnI GGCC 5 cut(s) 181, 260, 533, 783, 822
Bso31I GGTCTC 3 cut(s) 106, 209, 773
BsoBI CYCGRG 1 cut(s) 378
Bsp1286I GDGCHC 2 cut(s) 142, 713
Bsp13I TCCGGA 1 cut(s) 626
Bsp1407I TGTACA 1 cut(s) 6
Bsp143I GATC 3 cut(s) 363, 418, 588
BspANI GGCC 5 cut(s) 181, 260, 533, 783, 822
BspCNI CTCAG 1 cut(s) 820
BspEI TCCGGA 1 cut(s) 626
BspFNI CGCG 3 cut(s) 294, 296, 347
BspLI GGNNCC 5 cut(s) 578, 601, 659, 712, 815
BspMAI CTGCAG 1 cut(s) 997
BspPI GGATC 2 cut(s) 358, 583
BspT107I GGYRCC 1 cut(s) 813
BspTNI GGTCTC 3 cut(s) 106, 209, 773
BsrBI CCGCTC 3 cut(s) 147, 479, 596
BsrFI RCCGGY 2 cut(s) 278, 484
BsrGI TGTACA 1 cut(s) 6
BsrI ACTGG 1 cut(s) 394
BssAI RCCGGY 2 cut(s) 278, 484
BssECI CCNNGG 2 cut(s) 377, 817
BssMI GATC 3 cut(s) 363, 418, 588
Bst2UI CCWGG 3 cut(s) 493, 662, 765
Bst4CI ACNGT 1 cut(s) 75
BstAUI TGTACA 1 cut(s) 6
BstC8I GCNNGC 1 cut(s) 280
BstDEI CTNAG 4 cut(s) 809, 828, 843, 929
BstF5I GGATG 5 cut(s) 96, 372, 709, 862, 910
BstFNI CGCG 3 cut(s) 294, 296, 347
BstHHI GCGC 3 cut(s) 212, 273, 296
BstKTI GATC 3 cut(s) 366, 421, 591
BstMAI GTCTC 4 cut(s) 106, 209, 406, 773
BstMBI GATC 3 cut(s) 363, 418, 588
BstMCI CGRYCG 2 cut(s) 485, 489
BstMWI GCNNNNNNNGC 3 cut(s) 257, 279, 291
BstNI CCWGG 3 cut(s) 493, 662, 765
BstSCI CCNGG 4 cut(s) 491, 602, 660, 763
BstSFI CTRYAG 1 cut(s) 993
BstUI CGCG 3 cut(s) 294, 296, 347
BstV1I GCAGC 1 cut(s) 466
BstX2I RGATCY 1 cut(s) 588
BstXI CCANNNNNNTGG 1 cut(s) 668
BstYI RGATCY 1 cut(s) 588
BsuRI GGCC 5 cut(s) 181, 260, 533, 783, 822
BtsCI GGATG 5 cut(s) 96, 372, 709, 862, 910
BtsI GCAGTG 1 cut(s) 475
BtsIMutI CAGTG 2 cut(s) 387, 475
Cac8I GCNNGC 1 cut(s) 280
CaiI CAGNNNCTG 3 cut(s) 457, 505, 992
CfoI GCGC 3 cut(s) 212, 273, 296
Cfr10I RCCGGY 2 cut(s) 278, 484
Cfr13I GGNCC 5 cut(s) 180, 320, 600, 658, 821
CpoI CGGWCCG 1 cut(s) 320
Csp6I GTAC 2 cut(s) 7, 814
CspAI ACCGGT 1 cut(s) 484
CspCI CAANNNNNGTGG 2 cut(s) 760, 795
CspI CGGWCCG 1 cut(s) 320
CviAII CATG 2 cut(s) 919, 946
CviQI GTAC 2 cut(s) 7, 814
DdeI CTNAG 4 cut(s) 809, 828, 843, 929
DpnI GATC 3 cut(s) 365, 420, 590
DpnII GATC 3 cut(s) 363, 418, 588
EaeI YGGCCR 2 cut(s) 258, 781
EciI GGCGGA 1 cut(s) 716
Eco24I GRGCYC 2 cut(s) 142, 713
Eco31I GGTCTC 3 cut(s) 106, 209, 773
Eco47I GGWCC 3 cut(s) 320, 600, 658
Eco88I CYCGRG 1 cut(s) 378
EcoRI GAATTC 1 cut(s) 551
EcoRII CCWGG 3 cut(s) 491, 660, 763
EcoT38I GRGCYC 2 cut(s) 142, 713
FaeI CATG 2 cut(s) 922, 949
FaiI YATR 7 cut(s) 27, 638, 875, 920, 947, 953, 955
FaqI GGGAC 2 cut(s) 671, 716
FatI CATG 2 cut(s) 918, 945
FauI CCCGC 2 cut(s) 243, 589
Fnu4HI GCNGC 5 cut(s) 283, 348, 455, 474, 477
FokI GGATG 5 cut(s) 83, 359, 716, 869, 897
FriOI GRGCYC 2 cut(s) 142, 713
Fsp4HI GCNGC 5 cut(s) 283, 348, 455, 474, 477
FspBI CTAG 3 cut(s) 312, 465, 869
FspI TGCGCA 1 cut(s) 211
GlaI GCGC 3 cut(s) 211, 272, 295
GluI GCNGC 5 cut(s) 283, 348, 455, 474, 477
GsaI CCCAGC 3 cut(s) 437, 454, 827
GsuI CTGGAG 1 cut(s) 569
HaeIII GGCC 5 cut(s) 181, 260, 533, 783, 822
HapII CCGG 4 cut(s) 279, 485, 603, 627
HhaI GCGC 3 cut(s) 212, 273, 296
Hin1II CATG 2 cut(s) 922, 949
Hin6I GCGC 3 cut(s) 210, 271, 294
HinP1I GCGC 3 cut(s) 210, 271, 294
HindIII AAGCTT 1 cut(s) 923
HinfI GANTC 7 cut(s) 399, 429, 526, 623, 649, 676, 989
HpaII CCGG 4 cut(s) 279, 485, 603, 627
HphI GGTGA 1 cut(s) 761
Hpy166II GTNNAC 2 cut(s) 7, 9
Hpy188I TCNGA 6 cut(s) 151, 320, 593, 613, 681, 715
Hpy188III TCNNGA 6 cut(s) 380, 586, 627, 646, 869, 896
Hpy8I GTNNAC 2 cut(s) 7, 9
Hpy99I CGWCG 2 cut(s) 443, 613
HpyAV CCTTC 1 cut(s) 924
HpyCH4III ACNGT 1 cut(s) 75
HpyCH4IV ACGT 1 cut(s) 66
HpyCH4V TGCA 4 cut(s) 51, 304, 968, 995
HpyF10VI GCNNNNNNNGC 3 cut(s) 257, 279, 291
HpyF3I CTNAG 4 cut(s) 809, 828, 843, 929
HpySE526I ACGT 1 cut(s) 66
Hsp92II CATG 2 cut(s) 922, 949
HspAI GCGC 3 cut(s) 210, 271, 294
Kpn2I TCCGGA 1 cut(s) 626
KpnI GGTACC 1 cut(s) 817
KroI GCCGGC 1 cut(s) 278
KroNI GCCGGC 1 cut(s) 280
Kzo9I GATC 3 cut(s) 363, 418, 588
LmnI GCTCC 2 cut(s) 572, 716
Lsp1109I GCAGC 1 cut(s) 466
MaeI CTAG 3 cut(s) 312, 465, 869
MaeII ACGT 1 cut(s) 66
MaeIII GTNAC 1 cut(s) 385
MalI GATC 3 cut(s) 365, 420, 590
MbiI CCGCTC 3 cut(s) 147, 479, 596
MboI GATC 3 cut(s) 363, 418, 588
MboII GAAGA 2 cut(s) 685, 764
MflI RGATCY 1 cut(s) 588
MhlI GDGCHC 2 cut(s) 142, 713
MlsI TGGCCA 2 cut(s) 260, 783
MluCI AATT 8 cut(s) 40, 60, 79, 105, 307, 551, 682, 795
MluNI TGGCCA 2 cut(s) 260, 783
MlyI GAGTC 1 cut(s) 408
MmeI TCCRAC 2 cut(s) 591, 957
MnlI CCTC 9 cut(s) 215, 339, 376, 387, 510, 593, 700, 827, 844
Mox20I TGGCCA 2 cut(s) 260, 783
MroI TCCGGA 1 cut(s) 626
MroNI GCCGGC 1 cut(s) 278
MscI TGGCCA 2 cut(s) 260, 783
MseI TTAA 5 cut(s) 57, 63, 77, 191, 1003
Msp20I TGGCCA 2 cut(s) 260, 783
MspA1I CMGCKG 2 cut(s) 457, 827
MspI CCGG 4 cut(s) 279, 485, 603, 627
MspR9I CCNGG 4 cut(s) 493, 604, 662, 765
Mva1269I GAATGC 1 cut(s) 51
MvaI CCWGG 3 cut(s) 493, 662, 765
MvnI CGCG 3 cut(s) 294, 296, 347
MwoI GCNNNNNNNGC 3 cut(s) 257, 279, 291
NaeI GCCGGC 1 cut(s) 280
NciI CCSGG 1 cut(s) 604
NdeII GATC 3 cut(s) 363, 418, 588
NgoMIV GCCGGC 1 cut(s) 278
NlaIII CATG 2 cut(s) 922, 949
NlaIV GGNNCC 5 cut(s) 578, 601, 659, 712, 815
NmeAIII GCCGAG 1 cut(s) 798
NmuCI GTSAC 1 cut(s) 385
NsbI TGCGCA 1 cut(s) 211
PctI GAATGC 1 cut(s) 51
PdiI GCCGGC 1 cut(s) 280
PfeI GAWTC 6 cut(s) 429, 526, 623, 649, 676, 989
PflMI CCANNNNNTGG 1 cut(s) 267
PinAI ACCGGT 1 cut(s) 484
PkrI GCNGC 5 cut(s) 284, 349, 456, 475, 478
PleI GAGTC 1 cut(s) 407
PpsI GAGTC 1 cut(s) 407
Psp1406I AACGTT 1 cut(s) 66
Psp6I CCWGG 3 cut(s) 491, 660, 763
PspFI CCCAGC 3 cut(s) 433, 450, 823
PspGI CCWGG 3 cut(s) 491, 660, 763
PspN4I GGNNCC 5 cut(s) 578, 601, 659, 712, 815
PspPI GGNCC 5 cut(s) 180, 320, 600, 658, 821
PstI CTGCAG 1 cut(s) 997
PstNI CAGNNNCTG 3 cut(s) 457, 505, 992
PsuI RGATCY 1 cut(s) 588
PvuII CAGCTG 2 cut(s) 457, 827
RsaI GTAC 2 cut(s) 8, 815
RsaNI GTAC 2 cut(s) 7, 814
Rsr2I CGGWCCG 1 cut(s) 320
RsrII CGGWCCG 1 cut(s) 320
SaqAI TTAA 5 cut(s) 57, 63, 77, 191, 1003
SatI GCNGC 5 cut(s) 283, 348, 455, 474, 477
Sau3AI GATC 3 cut(s) 363, 418, 588
Sau96I GGNCC 5 cut(s) 180, 320, 600, 658, 821
SchI GAGTC 1 cut(s) 408
ScrFI CCNGG 4 cut(s) 493, 604, 662, 765
SduI GDGCHC 2 cut(s) 142, 713
SfcI CTRYAG 1 cut(s) 993
SinI GGWCC 3 cut(s) 320, 600, 658
SmlI CTYRAG 1 cut(s) 539
SmoI CTYRAG 1 cut(s) 539
Sse9I AATT 8 cut(s) 40, 60, 79, 105, 307, 551, 682, 795
SspMI CTAG 3 cut(s) 312, 465, 869
StyD4I CCNGG 4 cut(s) 491, 602, 660, 763
TaaI ACNGT 1 cut(s) 75
TaiI ACGT 1 cut(s) 69
TaqI TCGA 3 cut(s) 427, 481, 621
TasI AATT 8 cut(s) 40, 60, 79, 105, 307, 551, 682, 795
TatI WGTACW 1 cut(s) 6
TauI GCSGC 4 cut(s) 285, 350, 476, 479
TfiI GAWTC 6 cut(s) 429, 526, 623, 649, 676, 989
Tru1I TTAA 5 cut(s) 57, 63, 77, 191, 1003
Tru9I TTAA 5 cut(s) 57, 63, 77, 191, 1003
TscAI CASTG 2 cut(s) 394, 475
TseFI GTSAC 1 cut(s) 385
TseI GCWGC 1 cut(s) 454
Tsp45I GTSAC 1 cut(s) 385
TspDTI ATGAA 3 cut(s) 14, 899, 935
TspGWI ACGGA 1 cut(s) 394
TspRI CASTG 2 cut(s) 394, 475
Van91I CCANNNNNTGG 1 cut(s) 267
VpaK11BI GGWCC 3 cut(s) 320, 600, 658
XapI RAATTY 6 cut(s) 40, 79, 105, 307, 551, 682
XbaI TCTAGA 1 cut(s) 868
XcmI CCANNNNNNNNNTGG 1 cut(s) 253
XspI CTAG 3 cut(s) 312, 465, 869
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.